LOCUS       NM_012199              13712 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens argonaute RISC component 1 (AGO1), transcript variant
            1, mRNA.
ACCESSION   NM_012199
VERSION     NM_012199.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Fang,X., Ren,L.H., Shrestha,S.M., Ji,Q., Xu,Z., Wang,D., Ding,Q.,
            Liang,X. and Shi,R.H.
  TITLE     LINC01116 modulates EMT process via binding with AGO1 mRNA in
            oesophageal squamous cell carcinoma
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1870 (5), 119447 (2023)
   PUBMED   36990227
  REMARK    GeneRIF: LINC01116 modulates EMT process via binding with AGO1 mRNA
            in oesophageal squamous cell carcinoma.
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   Kowalczyk,M., Kowalczyk,E., Galita,G., Majsterek,I., Talarowska,M.,
            Poplawski,T., Kwiatkowski,P., Lichota,A. and Sienkiewicz,M.
  TITLE     Association of Polymorphic Variants in Argonaute Genes with
            Depression Risk in a Polish Population
  JOURNAL   Int J Mol Sci 23 (18), 10586 (2022)
   PUBMED   36142498
  REMARK    GeneRIF: Association of Polymorphic Variants in Argonaute Genes
            with Depression Risk in a Polish Population.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 13712)
  AUTHORS   O'Neill,A.C., Uzbas,F., Antognolli,G., Merino,F., Draganova,K.,
            Jack,A., Zhang,S., Pedini,G., Schessner,J.P., Cramer,K.,
            Schepers,A., Metzger,F., Esgleas,M., Smialowski,P., Guerrini,R.,
            Falk,S., Feederle,R., Freytag,S., Wang,Z., Bahlo,M., Jungmann,R.,
            Bagni,C., Borner,G.H.H., Robertson,S.P., Hauck,S.M. and Gotz,M.
  TITLE     Spatial centrosome proteome of human neural cells uncovers
            disease-relevant heterogeneity
  JOURNAL   Science 376 (6599), eabf9088 (2022)
   PUBMED   35709258
REFERENCE   4  (bases 1 to 13712)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 13712)
  AUTHORS   Eswarappa,S.M., Potdar,A.A., Koch,W.J., Fan,Y., Vasu,K.,
            Lindner,D., Willard,B., Graham,L.M., DiCorleto,P.E. and Fox,P.L.
  TITLE     Programmed translational readthrough generates antiangiogenic
            VEGF-Ax
  JOURNAL   Cell 157 (7), 1605-1618 (2014)
   PUBMED   24949972
REFERENCE   6  (bases 1 to 13712)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
REFERENCE   7  (bases 1 to 13712)
  AUTHORS   Martinez,J., Patkaniowska,A., Urlaub,H., Luhrmann,R. and Tuschl,T.
  TITLE     Single-stranded antisense siRNAs guide target RNA cleavage in RNAi
  JOURNAL   Cell 110 (5), 563-574 (2002)
   PUBMED   12230974
REFERENCE   8  (bases 1 to 13712)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   9  (bases 1 to 13712)
  AUTHORS   Koesters,R., Adams,V., Betts,D., Moos,R., Schmid,M., Siermann,A.,
            Hassam,S., Weitz,S., Lichter,P., Heitz,P.U., von Knebel
            Doeberitz,M. and Briner,J.
  TITLE     Human eukaryotic initiation factor EIF2C1 gene: cDNA sequence,
            genomic organization, localization to chromosomal bands 1p34-p35,
            and expression
  JOURNAL   Genomics 61 (2), 210-218 (1999)
   PUBMED   10534406
REFERENCE   10 (bases 1 to 13712)
  AUTHORS   Reddy,P.H., Stockburger,E., Gillevet,P. and Tagle,D.A.
  TITLE     Mapping and characterization of novel (CAG)n repeat cDNAs from
            adult human brain derived by the oligo capture method
  JOURNAL   Genomics 46 (2), 174-182 (1997)
   PUBMED   9417904
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY052874.1, BC063275.1,
            AK314148.1, AF093097.1, CR936823.1 and AL139286.13.
            
            On Nov 23, 2018 this sequence version replaced NM_012199.4.
            
            Summary: This gene encodes a member of the argonaute family of
            proteins, which associate with small RNAs and have important roles
            in RNA interference (RNAi) and RNA silencing. This protein binds to
            microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses
            translation of mRNAs that are complementary to them. It is also
            involved in transcriptional gene silencing (TGS) of promoter
            regions that are complementary to bound short antigene RNAs
            (agRNAs), as well as in the degradation of miRNA-bound mRNA
            targets. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. A recent study
            showed this gene to be an authentic stop codon readthrough target,
            and that its mRNA could give rise to an additional C-terminally
            extended isoform by use of an alternative in-frame translation
            termination codon. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes two isoforms, which result from the use of
            alternative in-frame translation termination codons. The shorter
            isoform (1) results from translation termination at the upstream
            UGA stop codon, while the longer isoform (1x) results from UGA stop
            codon readthrough to the downstream UAG termination codon. This
            RefSeq represents the shorter isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF093097.1, BC063275.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373204.6/ ENSP00000362300.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 HY052874.1         15-20
            7-936               BC063275.1         1-930
            937-2787            AK314148.1         912-2762
            2788-7478           AF093097.1         2788-7478
            7479-7479           CR936823.1         6081-6081
            7480-13712          AL139286.13        67508-73740         c
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..13712
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="argonaute RISC component 1"
                     /db_xref="GeneID:26523"
                     /db_xref="HGNC:HGNC:3262"
                     /db_xref="MIM:606228"
     exon            1..238
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..2787
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="isoform 1 is encoded by transcript variant 1; Golgi
                     Endoplasmic Reticulum protein 95 kDa; putative RNA-binding
                     protein Q99; protein argonaute-1; eIF2C 1; eIF-2C 1;
                     argonaute1; eukaryotic translation initiation factor 2C,
                     1; argonaute 1, RISC catalytic component; argonaute RISC
                     catalytic component 1"
                     /codon_start=1
                     /product="protein argonaute-1 isoform 1"
                     /protein_id="NP_036331.1"
                     /db_xref="CCDS:CCDS398.1"
                     /db_xref="GeneID:26523"
                     /db_xref="HGNC:HGNC:3262"
                     /db_xref="MIM:606228"
                     /translation="MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFE
                     VDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVT
                     ALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQAL
                     DVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVS
                     ATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRK
                     YRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKH
                     TYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYI
                     QEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWA
                     IACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKN
                     TYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKIN
                     VKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYC
                     ATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYEL
                     LAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITH
                     PFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIP
                     APAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYF
                     A"
     exon            239..422
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            423..543
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            544..725
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            726..862
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            863..997
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1085
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1233
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1353
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1476
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1610
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1795
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1955
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2046
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2241
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2376
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2478
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..2678
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..13712
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7449..7454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="hexamer: TATAAA"
     polyA_site      7479
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="major polyA site"
     regulatory      12767..12772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="hexamer: ATTAAA"
     polyA_site      12790
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="major polyA site"
     regulatory      13253..13258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="hexamer: AATAAA"
     polyA_site      13275
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
     regulatory      13689..13694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
                     /note="hexamer: ATTAAA"
     polyA_site      13712
                     /gene="AGO1"
                     /gene_synonym="EIF2C; EIF2C1; GERP95; hAgo1; NEDLBAS; Q99"
ORIGIN      
        1 actggcagct ggccgggcgc tcgcagtggg agctgctgca ggctccgcgg cggcggcaac
       61 ggaggctgcg ggggcggcgg cgcgagcggc cgggcttggt aggggagccg agcccggccc
      121 gggatcccga gcagcgagag tgtggggtac ctaggcccct cacgctggac ttcacagtct
      181 ccgggccgcc tgacctccgc acgggtatat gggatggaag cgggaccctc gggagcagct
      241 gcgggcgctt acctgccccc cctgcagcag gtgttccagg cacctcgccg gcctggcatt
      301 ggcactgtgg ggaaaccaat caagctcctg gccaattact ttgaggtgga catccctaag
      361 atcgacgtgt accactacga ggtggacatc aagccggata agtgtccccg tagagtcaac
      421 cgggaagtgg tggaatacat ggtccagcat ttcaagcctc agatctttgg tgatcgcaag
      481 cctgtgtatg atggaaagaa gaacatttac actgtcacag cactgcccat tggcaacgaa
      541 cgggtcgact ttgaggtgac aatccctggg gaagggaagg atcgaatctt taaggtctcc
      601 atcaagtggc tagccattgt gagctggcga atgctgcatg aggccctggt cagcggccag
      661 atccctgttc ccttggagtc tgtgcaagcc ctggatgtgg ccatgaggca cctggcatcc
      721 atgaggtaca cccctgtggg ccgctccttc ttctcaccgc ctgagggcta ctaccacccg
      781 ctggggggtg ggcgcgaggt ctggttcggc tttcaccagt ctgtgcgccc tgccatgtgg
      841 aagatgatgc tcaacattga tgtctcagcc actgcctttt ataaggcaca gccagtgatt
      901 gagttcatgt gtgaggtgct ggacatcagg aacatagatg agcagcccaa gcccctcacg
      961 gactctcagc gcgttcgctt caccaaggag atcaagggcc tgaaggtgga agtcacccac
     1021 tgtggacaga tgaagaggaa gtaccgcgtg tgtaatgtta cccgtcgccc tgctagccat
     1081 cagacattcc ccttacagct ggagagtgga cagactgtgg agtgcacagt ggcacagtat
     1141 ttcaagcaga aatataacct tcagctcaag tatccccatc tgccctgcct acaagttggc
     1201 caggaacaaa agcataccta ccttccccta gaggtctgta acattgtggc tgggcagcgc
     1261 tgtattaaaa agctgaccga caaccagacc tcgaccatga taaaggccac agctagatcc
     1321 gctccagaca gacaggagga gatcagtcgc ctgatgaaga atgccagcta caacttagat
     1381 ccctacatcc aggaatttgg gatcaaagtg aaggatgaca tgacggaggt gacagggcga
     1441 gtgctgccgg cgcccatctt gcagtacggc ggccggaacc gggccattgc cacacccaat
     1501 cagggtgtct gggacatgcg ggggaaacag ttctacaatg ggattgagat caaagtctgg
     1561 gccatcgcct gcttcgcacc ccaaaaacag tgtcgagaag aggtgctcaa gaacttcaca
     1621 gaccagctgc ggaagatttc caaggatgcg gggatgccta tccagggtca accttgtttc
     1681 tgcaaatatg cacagggggc agacagcgtg gagcctatgt tccggcatct caagaacacc
     1741 tactcagggc tgcagctcat tattgtcatc ctgccaggga agacgccggt gtatgctgag
     1801 gtgaaacgtg tcggagatac actcttggga atggctacgc agtgtgtgca ggtgaagaac
     1861 gtggtcaaga cctcacctca gactctgtcc aacctctgcc tcaagatcaa tgtcaaactt
     1921 ggtggcatta acaacatcct agtcccacac cagcgctctg ccgtttttca acagccagtg
     1981 atattcctgg gagcagatgt tacacacccc ccagcagggg atgggaaaaa accttctatc
     2041 acagcagtgg taggcagtat ggatgcccac cccagccgat actgtgctac tgtgcgggta
     2101 cagcgaccac ggcaagagat cattgaagac ttgtcctaca tggtgcgtga gctcctcatc
     2161 caattctaca agtccacccg tttcaagcct acccgcatca tcttctaccg agatggggtg
     2221 cctgaaggcc agctacccca gatactccac tatgagctac tggccattcg tgatgcctgc
     2281 atcaaactgg aaaaggacta ccagcctggg atcacttata ttgtggtgca gaaacgccat
     2341 cacacccgcc ttttctgtgc tgacaagaat gagcgaattg ggaagagtgg taacatccca
     2401 gctgggacca cagtggacac caacatcacc cacccatttg agtttgactt ctatctgtgc
     2461 agccacgcag gcatccaggg caccagccga ccatcccatt actatgttct ttgggatgac
     2521 aaccgtttca cagcagatga gctccagatc ctgacgtacc agctgtgcca cacttacgta
     2581 cgatgcacac gctctgtctc tatcccagca cctgcctact atgcccgcct ggtggctttc
     2641 cgggcacgat accacctggt ggacaaggag catgacagtg gagaggggag ccacatatcg
     2701 gggcagagca atgggcggga cccccaggcc ctggccaaag ccgtgcaggt tcaccaggat
     2761 actctgcgca ccatgtactt cgcttgaagg cagaacgctg ttacctcact ggatagaaga
     2821 aagctttcca agccccagga gctgtgccac ccaaatccag aggaagcaag gaggagggag
     2881 gtggggtagg gaggagtgta ggatgccttg tttccttcta tagaggtggt gtaagagtgg
     2941 ggaacagggc cagcaagaca gaccaccagc cagaaatctc tgatatcaac ctcatgtccc
     3001 ccacccctca ccccatcttg tcacatctgg ccctgacccc actggaccaa aaggggcagc
     3061 actggtgccc accatacaca caggtgtctc atgtgactca cagtgctaaa gactcatgct
     3121 tgacagcttg gtaaggtcaa ctctgtagcc ctgcagacaa aagctggtta ggtttgggtt
     3181 tgatacttta gatgggaaag tgaggggctt gagaaagtgg gtgggaggag ggaaggattt
     3241 tttaggagcc ttaatcagaa aaggactaga tttgtttaag aagaaaaatg aaaccagacc
     3301 cagatcaata ttttaggata ctagatgttt taatgggttc agaatccagt ttgtaggaag
     3361 attttttaat ggttttggtt gctcctcccc cagctgccac cccccacctt acccttattc
     3421 ctctctgtcc acattttctg ccccacctta cttctcctcc ctgacagaca tccagcccct
     3481 agtaatactt aaggcactat ggcacttagc tttgaagtga cacgaccctg tcttccttcc
     3541 gcccgctggt gggtaaccag tgccttccct gtaacggtaa tgctgcagaa ctgcaacctt
     3601 ttgtaccttt ctttggggaa tggggtgggg gtgggagagg aggtagatgg ggaagaaata
     3661 ccccagaccc aacaaacctc cagccagaaa gccagctatt ttgcatttga aggaattgac
     3721 ttcctcattc attgagcttt ttaaaagatc acaacctcaa gatggttaaa atccattgac
     3781 atttgcactt tcaaacatga caagtctcgg agctgctgag atgacaggcc cctggccttt
     3841 ccacttatgc ctccttttct ccttattcct cctacctccc gccccgccca ggtctggagt
     3901 tactttcata gcatttttca ctcttggctt cttttctccc ttgatggtca agtctcttat
     3961 gtttcaatat ttcttaactg gggtgtctta taacaaaaaa ctcttaggtc taaaatgaga
     4021 aaaaagagag aaaacaaaat gttattttta taccataact tgagtgtatt gccaaaattt
     4081 ggaaatcctt cccatgcctg atgagtttat atcccagaaa cattgagcca tcagaatgaa
     4141 ctgtgtacct gatttgttct ctgacctggc taggtaggga gggggtggtt atcgccccaa
     4201 gatggggtcc aggctccatc cttcctctgt gcagataata cctttttctt gctatagcct
     4261 ccctcctctg cactgtcctg cactctttct tgcaagtgca tctttttcct tcccctggac
     4321 tgtcctctga ccctttggct catcctagat tgcagtgtgt cctgtggaca ggctggggaa
     4381 ttttgctgct ccctattgct tctgtttaca aaaatgaatt tttcctggtt tcccactagg
     4441 gcatgtgggt gggtggcatg gacttttttt tttttttttt tttgtcttga gacatggggt
     4501 ttggctgtct tgcaggactg gagaaggtgg tggttctagc ttggtctctg ttggccttga
     4561 agcaagcatc ccccctgccc tttttccttg actgttcatt tttttcctgc cccactgctt
     4621 gggatgggga gttgcaactt cagtgtggaa tttcctcttt gaggagcctg ggcttggatc
     4681 tatcctgatc tggtgatgaa gccatgatta ctttagacct agcccaggct tggaggccag
     4741 ctggaggaag aagggtctaa atcctggcct gtagagttag aactaccatt tcctcccctt
     4801 agctgccctt gtatgacccg gatttgctat gcaaaacaat ctatcccagg ttctgttctg
     4861 gttggctaca ttgttcagca actcacaaaa cgtagcacaa acattcatta tggagaaagc
     4921 atcaggactg ttgagtaact cctcctttac ttttttcctg ctggctacag catggggtgc
     4981 cctataggca caagcccagc tgaagaacag aatggagggc tctgggagga ggcagctcac
     5041 tggagagcct acattcctta cacaagtgcc taaagagagt gatgctaaca ctccatctgc
     5101 cctgtccatt gccttcatat acagtctact tcgtgttctg tcaccctttg gggaggggag
     5161 ttctcctggg acagtgggct ctgcatgttc tccacttgga tacattttgg ggctaggatc
     5221 agggcactat tcctggaggg tccagtcatt caccagcatt tgcaaatgtc catagggagc
     5281 aggtggcagc ctctactccc agcaacaagt ttgtgttctc tccttttctc tctttgcctc
     5341 actctctcca gttggttttc agctggggct tgaaatgcat ttttagccct ttgacgtggc
     5401 ttatgccatt caagaaataa aaagcaagag aatcagcttt gggcaatgac aagaaatgag
     5461 ttcttactct gatttttttg taaaaagata atttttgaga cttgaaaaat accccgacct
     5521 tgagattatt cctgtttgaa aggtggtgca tgcagatgga gaagtggtgt tggcagcaag
     5581 ctttggctca tgtggatttg gtttaagtgg tgcttcttac ccaagcttca aggaagtgct
     5641 tgggggaccc ccagcctcat cctcttagtt gggtctcttg ttccctttgt accactgttt
     5701 tgccttcctt ttcctcttct ctctttgcct ggcttccttt cccttttctt ctattcactc
     5761 tgcttgcttg ctggccggcc tgcctgcctg cctgcctgcc tgcctgcctg tctgcctatg
     5821 tgatgatgaa atctctgcat ggctgcaatg atcccactgt tagctggcag ggtcaggctt
     5881 agctccttga ctgcagaaga ccaagaacct gttccccaag cccagagatg tccacctggg
     5941 ctggactgcc ctcaagctta tactagagaa gagcaactga cctgcccaac ttgtgtgaag
     6001 tcaggagggt ttctggcatt ttccacacct gtccactcct tggagctggt ttctctcatt
     6061 gctttttcta aatctggttc tttttctctt tacctggggc ctggcttttc tgagattgtc
     6121 ttagggttga gctatttggg tatcctgggt ttgagtgtta ggggatggac ataaaggaaa
     6181 aagagtgatg agaagagaat ggagagaatt tgaataaaag gtgggaaagg agagcactgt
     6241 tctttgattg tttatccagt ccaacctgat ccattaggga tcgaggtgct acactggcct
     6301 ccagggataa gcctggggct actgttgctg ggaacttagg cttaacataa agccgaagaa
     6361 ggtacctaga aatttgaaac ttccctaaaa agctcctaat gcccacctgc tagatagctt
     6421 ctctgtggcc tcctatttag ctaagcagca gtgtttttgg atactttttt tttctgtttg
     6481 tgaataaggc cagcactcaa gatgggcagc caagggtgca ctgactatta gctggcccat
     6541 aggatatctg taaggctggt gggacagttt tggacctgga atcatgtgta actaacaagg
     6601 ttggacgttt cttccccatc agggtagaaa aatcatctca aactagccaa aaggcagttt
     6661 tggaaactac attgggggac gttattttta tttatatatg gggcctaggc caatccagga
     6721 tggtagctgg aataccttcc ttcttaaaat ctgatcatgg cagggatatg cagggcactt
     6781 tttactattt ggccttctaa gcagattggg aaggaggtat tttctggttt tcgctttcct
     6841 ccgacttaat aggacttgcc ttctccctgg gcagggagag aggctgggtt ggtgctctcc
     6901 cttactctac tcatactgac ttagagcctc tggctgctgt ttgggcatcc aagaaaggga
     6961 ggggaaggaa tgagctaaaa acaaaacaga atgaggtggg aaagggagat tttcttcttt
     7021 acagaggaaa ataggaaacc ctccaagaat tgtgcaagta aagacatttg ttgaatgcac
     7081 tgagtccctt ggtgtagtag caataaggaa aaatgaaatt actttcctgt gcacacagtc
     7141 cagcctaatt ggtatgtgat gttgcactta gcagccatgt ggtgggcatg tgtgactact
     7201 ctggttttca ctttagtttc taaacttttt atccctctca agtccagcat ggatggggaa
     7261 atgtctctgg atccccacag ctgtgtactt gtttgcattt gtttcccttt gagatttgtg
     7321 tttgtgtcct gctttgagct gtaccttgtc cagtccattg tgaaattatc ccagcagctg
     7381 taatgtacag ttccttctga agcaagcaac atcagcagca gcagcagcag cagcacaatt
     7441 ctgtgtttta taaagacaac agtggcttct atttctaaag tgcggtcttt ctcttttttt
     7501 ttcctaccag caaaacaaac ttttgggact gattacatct ctaatagatt ttaggtgaga
     7561 ataatactgt agattgttat gcaggaatac ttcacagagc cttcatttat tcttcattca
     7621 acaaacatgc aaagcactgt gccagcagta ttgtggggag gggaggcaca attcaaaatg
     7681 aggaaaatag tgtccgtctc attaagggaa ttaagtttgg tgggggatat gattagccaa
     7741 atagtcccct ggcataggag gaagataatg agggagtgga ataaggctac aacaacgaat
     7801 atagggagga agggacagat ttgagagacg aggtagaatt aataggactc gatggctggt
     7861 gggaggagaa gacaggagta gaggttagct cccaggtttc tccttgacca taggagtgtg
     7921 ttgggacatt ctgccagtca agatgggggt gacggggaga ctgtagaagg aaggtgggga
     7981 gtttttgaag aaacagaatg ttgtatagac tgagttttga ggtgtttgtg gggcagtaag
     8041 ggtaaggtgt ccagtaaaca caggttggtg ctcaggtaag actgtaaaac tgcatttata
     8101 gatacaggag tcttatagat ggtagttaaa gccataggca tgaatgagat agcttagaaa
     8161 aagagaagag aaactagtat acagccccct aagaaactca atttaaaggt tcgggggagg
     8221 aagcagatct taaggtgaca gatcactggt agacagtttg tgggtttttt gtttctttgt
     8281 ttagccagtt tggtgaggta ggagaagaaa tcagagtaga agaaggttca tgaagggagt
     8341 gattaacaac atgaactgct gcagagaggg agttcttttt ttttctgtgt gttttacctt
     8401 tctactcccc atcttttggg gatcttgtaa ctctatgact tacttacgtt attctccagt
     8461 atttcttgaa aatgagcatt ggaaaaacca attctaaaat ggctaaaact aggactttca
     8521 agttcaccac aactaccacc aattaggaga taattgtaaa agaataaaca aaacctaatt
     8581 ttgttcctag ccaatattat aaaccatgtt ccagcatact agataatagt agcatactat
     8641 atttatttcc ataatttgtc ttctttagag cctatgaact cctaggagca gaagctatat
     8701 tctcttcatt tttgtcatct cagggtcatg gcatatacca acagatcagt atgtgcaaat
     8761 ataattgaaa taggctagta gtctgtggtt tatgagtatg ggctgggtgg gcagtggatc
     8821 aagaagtgat aggagtacac aggatgaact cagaattcct gaatcttcag gtaagcagat
     8881 acttaactac attatcattg tcttttccca ctcaccccaa gttggaggtt ctatggtttt
     8941 gtactttaag ctggtggtat aattgtcaag catgtaaatt ctggcattct tcctggtact
     9001 gctagagcat tgttatttct atgcatgggg gtaagaaggc tcagagaagg atcctggcgg
     9061 ataccccagt gagaaatcgt cagtggcagc cagtgtgtac atacgtggac ctagcattcc
     9121 tctggaggcc aagtgttgta aattggcctg tggctatcat ctggcagact cccttgggaa
     9181 agagccagcc agccccatgc ttcttttcag cagcaagggt tagggaggtt ggttgaactt
     9241 gaagagcaga ttggacatgg agcagatttt gaccagttga aataagacat aaatttatga
     9301 ctccgagtga ggcagaaata aagaacttag cagggggaat aggaggagga tgattatggc
     9361 aaattttgct ataaacttta gttttagaaa agattgatat tgaaaagcct tcaggatttg
     9421 cctgtggttg ctactcaagt taaaaagact gggagcctgc attgtttggt cctggagctc
     9481 aaggttttca gtgaaggctt gtcctggcac ctctgggcat tccttttcat tactggttga
     9541 gcatccttcc ttctgctctg tcaattggca aaatatgctg gagcacattc tggactagca
     9601 gttgccttgg ggtagtcagg ctgggtattt gtttggtatc tcttggtgta gcagacccca
     9661 gaatctcatg gcaattagga tttggtggct aaacgaaaga gttagtgcaa ggaaaagcct
     9721 agttggaatt tctgagtctg ggccatcctt aagctgctgt ctactgcctg aatgggaagt
     9781 aatgtcgaat tggaaaatta gctcaccatt tttgttctag ctttgcagac atttattcct
     9841 ctgatgatag gctgagaaat gcctaggccg gcttagaagg cacacagcat gacagaagta
     9901 ctccttcaaa gcagctgtgt caagaaggaa gagctggaaa agtggatttg ctggggaatt
     9961 agatgcattt agttttccac ttacgcacaa ctgccttctc cagtatatca taccaaggtt
    10021 ttttaggcct ttgtggctta ccctgcagga acatactgtg tcctgttgtt ctggactgta
    10081 gcatcttcac caccatcctc ttggcaatgg ttttttgttt ggggtttttt tttttttttt
    10141 tttggacaaa gtataataat attgagggac ctttctaggg agcctttggt cctttgtccc
    10201 cattttccaa agggagggaa ctttcctctt aaaagagaca gtgggaactt ttagccaggt
    10261 ttttggaaaa ggcctgtggg ctattaatga gagtgagctg caaaaggagg aagggtttgt
    10321 ttgggtactt tgaagtactt agctaggtgt tttctacagt atcttatata attgcatctt
    10381 acagcatctt tacatgcgaa atagtttatc agttatcttc aattgcatat tacaaaattc
    10441 tgattaaatt tttcttaaat aagaaaacta tcatttcata tgacaagaaa tcctaattta
    10501 atgttactcc aaggttggtt aattcagtgg ctcagtgata acaccaaaga ctcagtttct
    10561 tgaaattttt ttgctctgcc atcctccatg tttatcctca cgccagttcg cctcatggtc
    10621 ccaatatggc tactgtaatt ctgggaagga aatgctgaca gaaccgtgtc tggcaaaaga
    10681 gagacagatt tttctgtgtc cttctaaagc aaggaagtct tcccctggaa gctccaacag
    10741 atacggcctc atggctttgc tagaaatggg tcatctcact cctgtcattg gcaaagcaag
    10801 taagatcatc catcaggagt ggcttagatt aatgaattat cctttgagtc gtgtagacta
    10861 gaaagttcag tcatgtctac tagcaagaaa ggagataagg gctgttggat aagcagcatc
    10921 tggtctcaaa ggtacttcaa cttttacaga tgagaaacat gaatcagagt gactttccca
    10981 ggctcacagc aagtaagaga gggccttcag tgttagctgt gtctgtccct aaagcccatg
    11041 ttttatattg tatcttgcta gagtataaag taacttactt tctaccttgt agaaactgat
    11101 ggtctaggag aagagatttg aactcaggtg cccagttgac tctaatcttg gcccatgtga
    11161 gaggattgta ctcagttgct ctcctcatcc agtgatgttt cttgtctaac acaagaagcc
    11221 taggagtaga aagtcctaag cagagtagaa taatagcggc agcagggagg gaagtgtgaa
    11281 ctgtgtgtcc ctgagcctga cttactgggc agggacatga cacttaaagc ggcagctggc
    11341 cactctgtct ttcctgcatg cactgagtat ttggtagctc attagtgacc agtgatattg
    11401 atcatctttt catgtgctca cttactatcc ccatatcttt gttgtttgca tcttttaccc
    11461 attgtatttg ttgtttttga gacagtgtct cactctgtca cccaggctgg agtgcagtct
    11521 catgatcgcg gctcactgca gtttctgcct cctgggctca agcaatcctc ccacctcagc
    11581 ctcctgagta gctgggctta caggcgcaca ccaccatgcc cagctaatta aaaaaaaaat
    11641 tttttttgta gcagttcggt ctcattagta ttgcccaggc tggcctctct tagcttcaag
    11701 ctatcctccc gcctcggcct cccaaagtgc tggaattaca ggcataagcc acaagccact
    11761 gggcccagcc tcttttacct gtttcaaaat tgagttttat tgagttgtaa gagttcttta
    11821 ttcattttaa acacaagtcc tttgttggat atatctttta catctatttt tcccagtctg
    11881 tggcttgcct tttcattttg aagagcaaaa gtttaaaatt ttgtgaattt attaaagctc
    11941 cgtttgttgc ttttttctgt tctagtttat acttttgtat catattttag gaaattttgc
    12001 actgggtatt tgatggctcc tgaggaggtt caactttcag agcgcactga tttctctgca
    12061 cctgagattt tgtactattt ctgtatttct ttcttctcag aacaaccagt gtcaccaggt
    12121 atgagggcag agttttagct tgtttgctga gccccattct tgaagctcat ttatttattc
    12181 acctatctgt ccatcaatcc aacaaatata ctgaatgctg ctatgtgcca ggtactggca
    12241 ctgttctagg tactggggca atgacagtta agataatacc caatgaccct gctctgtacc
    12301 cttaagagca gactcagttg ggaatgagtt atccaaatat agggtgtaca tgtagtcagg
    12361 agagagcctc atacagcttt gcctttggca gaatccttca aacctctttg tcttcctact
    12421 tcttgatatt acaaatcatg agcctttcac atgcattgac tctaaagagg tgggttcctg
    12481 gatagcagaa gtagatagga aggcatcatt gacatttact gagatggatt gaatcagagg
    12541 gtgtaaattc tgctccatca atgttgaaaa agactagccc accccaaagg tttatcactg
    12601 tcaccatgtc taatgtctat tctggaagaa gctgaagaag tctgcctttg tttagggaac
    12661 cggttccaga atcagagggg ttgaggatat aaaagaccct tgcacaagag ttggtattta
    12721 ccctgtcaga tgctgctacc aagatttgga ttgtgtacag tcgaggatta aaaatgactt
    12781 ttaagatgca gtgtttcatc tggtgacatt taatttgata ttttaaaatt ggggagggta
    12841 ttttcagcca tgggggtagg gatgtgaaag aagaagaaag taagaattgg agtcagatgt
    12901 ggttttgagt cccatacatt tattgtttaa tattatatgg taagtacttt aaaatggtaa
    12961 atagtaaata cttgtgttat tcactaacat ttggggagga ttttctctgt gctttatgta
    13021 tattacctaa ccttacaaac tgtgtaaggt acatgctgtt ctctcaattt ttcagataag
    13081 aaaattgaag cccagagaga tcaagtgact gatccaaagt cccacagtga ggggaactga
    13141 agataggata ttctttctcc ttttcctctt taaaaaatta ttttgattaa aaaaaaccgt
    13201 attactgaag catagggaca tggggagaaa ttatgaatga ttcctcagcc tgaataaaat
    13261 agtatgtttc attaaggcaa caaatattta ttaaggacct aatatttgcc tgacattcaa
    13321 gtgaggtggg gggcacacaa gtagtaaata aataacagac tgggcaaagg gctaccaagg
    13381 caatactgta gtagactaac aggtgcccca ccatagatgg gtggtccgga aaaggcgtct
    13441 cagatgtctt tcgttgagac gcaaaaaaaa gctgtggggt agggcgtaag gggagggtgg
    13501 agaagagaga gagggttcct ggccaaggga accaaaagca gagaagctcc aagtcagaaa
    13561 aaagcagcgg ttggcccgtt gggagaattg gaagccaacc agtatagctg tagcacaggc
    13621 ggggcccatt tacgccgtgc gttttcaccc tggaaaggag tttgggtttt caagtgtgac
    13681 gaggcgctat taaagagttt taaacatgga aa
//
