LOCUS       NM_000642               7091 bp    mRNA    linear   PRI 20-OCT-2024
DEFINITION  Homo sapiens amylo-alpha-1,6-glucosidase and
            4-alpha-glucanotransferase (AGL), transcript variant 1, mRNA.
ACCESSION   NM_000642
VERSION     NM_000642.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Perveen,S., Gupta,N., Kumar,M., Kaur,P., Chowdhury,M.R. and
            Kabra,M.
  TITLE     Spectrum of amyloglucosidase mutations in Asian Indian patients
            with Glycogen storage disease type III
  JOURNAL   Am J Med Genet A 182 (5), 1190-1200 (2020)
   PUBMED   32222031
  REMARK    GeneRIF: Spectrum of amyloglucosidase mutations in Asian Indian
            patients with Glycogen storage disease type III.
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Abdullah,I.S., Teh,S.H., Khaidizar,F.D., Ngu,L.H., Keng,W.T.,
            Yap,S. and Mohamed,Z.
  TITLE     Intron retention is among six unreported AGL mutations identified
            in Malaysian GSD III patients
  JOURNAL   Genes Genomics 41 (8), 885-893 (2019)
   PUBMED   31028654
  REMARK    GeneRIF: Six novel mutations were identified in GSD III patients.
REFERENCE   3  (bases 1 to 7091)
  AUTHORS   Nazari,F., Sinaei,F., Nilipour,Y., Petit,F., Oveisgharan,S.,
            Nassiri-Toosi,M., Razzaghy-Azar,M., Mahmoudi,M. and Nafissi,S.
  TITLE     Distinct Clinical and Genetic Findings in Iranian Patients With
            Glycogen Storage Disease Type 3
  JOURNAL   J Clin Neuromuscul Dis 19 (4), 203-210 (2018)
   PUBMED   29794575
  REMARK    GeneRIF: This report of patients with GSD-III in Iran with 2
            uncommon clinical presentations and 5 novel mutations in the AGL
            gene.
REFERENCE   4  (bases 1 to 7091)
  AUTHORS   Lu,C., Qiu,Z., Sun,M., Wang,W., Wei,M. and Zhang,X.
  TITLE     Spectrum of AGL mutations in Chinese patients with glycogen storage
            disease type III: identification of 31 novel mutations
  JOURNAL   J Hum Genet 61 (7), 641-645 (2016)
   PUBMED   26984562
  REMARK    GeneRIF: The study identified 31 novel mutations and extended the
            mutation spectrum of AGL in Chinese patients with glycogen storage
            disease type III.
REFERENCE   5  (bases 1 to 7091)
  AUTHORS   Massari,F., Ciccarese,C., Santoni,M., Iacovelli,R.,
            Mazzucchelli,R., Piva,F., Scarpelli,M., Berardi,R., Tortora,G.,
            Lopez-Beltran,A., Cheng,L. and Montironi,R.
  TITLE     Metabolic phenotype of bladder cancer
  JOURNAL   Cancer Treat Rev 45, 46-57 (2016)
   PUBMED   26975021
  REMARK    GeneRIF: AGL loss causes high SHMT2 expression and consequently
            increases glycine-dependent nucleotide synthesis leading to bladder
            cancer growth.
            Review article
REFERENCE   6  (bases 1 to 7091)
  AUTHORS   Schreuder,A.B., Rossi,A., Grunert,S.C. and Derks,T.G.J.
  TITLE     Glycogen Storage Disease Type III
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301788
REFERENCE   7  (bases 1 to 7091)
  AUTHORS   Yang-Feng,T.L., Zheng,K., Yu,J., Yang,B.Z., Chen,Y.T. and Kao,F.T.
  TITLE     Assignment of the human glycogen debrancher gene to chromosome 1p21
  JOURNAL   Genomics 13 (4), 931-934 (1992)
   PUBMED   1505983
REFERENCE   8  (bases 1 to 7091)
  AUTHORS   Yang,B.Z., Ding,J.H., Enghild,J.J., Bao,Y. and Chen,Y.T.
  TITLE     Molecular cloning and nucleotide sequence of cDNA encoding human
            muscle glycogen debranching enzyme
  JOURNAL   J Biol Chem 267 (13), 9294-9299 (1992)
   PUBMED   1374391
REFERENCE   9  (bases 1 to 7091)
  AUTHORS   Ding,J.H., de Barsy,T., Brown,B.I., Coleman,R.A. and Chen,Y.T.
  TITLE     Immunoblot analyses of glycogen debranching enzyme in different
            subtypes of glycogen storage disease type III
  JOURNAL   J Pediatr 116 (1), 95-100 (1990)
   PUBMED   2295969
REFERENCE   10 (bases 1 to 7091)
  AUTHORS   Katsarkas,A. and Younge,B.R.
  TITLE     Electronystagmographic findings in cerebellar dysfunction
  JOURNAL   J Otolaryngol 5 (2), 143-153 (1976)
   PUBMED   933239
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U84007.1, BC078663.1,
            AC096949.2 and AK130308.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000642.2.
            
            Summary: This gene encodes the glycogen debrancher enzyme which is
            involved in glycogen degradation. This enzyme has two independent
            catalytic activities which occur at different sites on the protein:
            a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase
            activity. Mutations in this gene are associated with glycogen
            storage disease although a wide range of enzymatic and clinical
            variability occurs which may be due to tissue-specific alternative
            splicing. Alternatively spliced transcripts encoding different
            isoforms have been described. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U84007.1, SRR1660809.258405.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361915.8/ ENSP00000355106.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               U84007.1           278-390
            114-5026            BC078663.1         111-5023
            5027-5161           U84007.1           5301-5435
            5162-6296           AC096949.2         23727-24861         c
            6297-6514           AK130308.1         1000-1217
            6515-7033           U84007.1           6790-7308
            7034-7091           AK130308.1         1738-1795
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p21.2"
     gene            1..7091
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /note="amylo-alpha-1,6-glucosidase and
                     4-alpha-glucanotransferase"
                     /db_xref="GeneID:178"
                     /db_xref="HGNC:HGNC:321"
                     /db_xref="MIM:610860"
     exon            1..55
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            56..205
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..4722
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /EC_number="2.4.1.25"
                     /EC_number="3.2.1.33"
                     /note="isoform 1 is encoded by transcript variant 1;
                     amylo-1, 6-glucosidase, 4-alpha-glucanotransferase;
                     glycogen debranching enzyme; glycogen debrancher;
                     amylo-alpha-1, 6-glucosidase,
                     4-alpha-glucanotransferaseprovided; glycogen debranching
                     protein"
                     /codon_start=1
                     /product="glycogen debranching enzyme isoform 1"
                     /protein_id="NP_000633.2"
                     /db_xref="CCDS:CCDS759.1"
                     /db_xref="GeneID:178"
                     /db_xref="HGNC:HGNC:321"
                     /db_xref="MIM:610860"
                     /translation="MGHSKQIRILLLNEMEKLEKTLFRLEQGYELQFRLGPTLQGKAV
                     TVYTNYPFPGETFNREKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKS
                     GGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHF
                     TPLQTLGLSRSCYSLANQLELNPDFSRPNRKYTWNDVGQLVEKLKKEWNVICITDVVY
                     NHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWRFSCDVAEGKYKEKGIPALIE
                     NDHHMNSIRKIIWEDIFPKLKLWEFFQVDVNKAVEQFRRLLTQENRRVTKSDPNQHLT
                     IIQDPEYRRFGCTVDMNIALTTFIPHDKGPAAIEECCNWFHKRMEELNSEKHRLINYH
                     QEQAVNCLLGNVFYERLAGHGPKLGPVTRKHPLVTRYFTFPFEEIDFSMEESMIHLPN
                     KACFLMAHNGWVMGDDPLRNFAEPGSEVYLRRELICWGDSVKLRYGNKPEDCPYLWAH
                     MKKYTEITATYFQGVRLDNCHSTPLHVAEYMLDAARNLQPNLYVVAELFTGSEDLDNV
                     FVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDI
                     THDNECPIVHRSAYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNP
                     EALPSNTGEVNFQSGIIAARCAISKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIH
                     QSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTIERNTKPYRKDENSING
                     TPDITVEIREHIQLNESKIVKQAGVATKGPNEYIQEIEFENLSPGSVIIFRVSLDPHA
                     QVAVGILRNHLTQFSPHFKSGSLAVDNADPILKIPFASLASRLTLAELNQILYRCESE
                     EKEDGGGCYDIPNWSALKYAGLQGLMSVLAEIRPKNDLGHPFCNNLRSGDWMIDYVSN
                     RLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDTAWKQMSSFV
                     QNGSTFVKHLSLGSVQLCGVGKFPSLPILSPALMDVPYRLNEITKEKEQCCVSLAAGL
                     PHFSSGIFRCWGRDTFIALRGILLITGRYVEARNIILAFAGTLRHGLIPNLLGEGIYA
                     RYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVI
                     QEAMQKHMQGIQFRERNAGPQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDK
                     MGESDRARNRGIPATPRDGSAVEIVGLSKSAVRWLLELSKKNIFPYHEVTVKRHGKAI
                     KVSYDEWNRKIQDNFEKLFHVSEDPSDLNEKHPNLVHKRGIYKDSYGASSPWCDYQLR
                     PNFTIAMVVAPELFTTEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDN
                     YNLAKGFNYHQGPEWLWPIGYFLRAKLYFSRLMGPETTAKTIVLVKNVLSRHYVHLER
                     SPWKGLPELTNENAQYCPFSCETQAWSIATILETLYDL"
     exon            206..416
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            417..583
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            584..787
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            788..969
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1081
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1205
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1308
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1406
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1546
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1734
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1858
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..2022
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2124
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2280
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2431
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2556
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2669
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2670..2804
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2805..2935
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3072
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3073..3206
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3382
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3383..3485
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3486..3711
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3712..3823
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..3959
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4072
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4284
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            4285..4382
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            4383..4470
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            4471..4604
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     exon            4605..7091
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7069..7074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /note="hexamer: AATAAA"
     polyA_site      7091
                     /gene="AGL"
                     /gene_synonym="GDE"
                     /note="major polyA site"
ORIGIN      
        1 accagagtcg cgggtcctgc agtgccccag aagccgcacg tataactccc tcggcgggta
       61 actcattcga ctgtggagtt cttttaattc ttatgaaaga tttcaaatcc tctagaagcc
      121 aaaatgggac acagtaaaca gattcgaatt ttacttctga acgaaatgga gaaactggaa
      181 aagaccctct tcagacttga acaagggtat gagctacagt tccgattagg cccaacttta
      241 cagggaaaag cagttaccgt gtatacaaat tacccatttc ctggagaaac atttaataga
      301 gaaaaattcc gttctctgga ttgggaaaat ccaacagaaa gagaagatga ttctgataaa
      361 tactgtaaac ttaatctgca acaatctggt tcatttcagt attatttcct tcaaggaaat
      421 gagaaaagtg gtggaggtta catagttgtg gaccccattt tacgtgttgg tgctgataat
      481 catgtgctac ccttggactg tgttactctt cagacatttt tagctaagtg tttgggacct
      541 tttgatgaat gggaaagcag acttagggtt gcaaaagaat caggctacaa catgattcat
      601 tttaccccat tgcagactct tggactatct aggtcatgct actcccttgc caatcagtta
      661 gaattaaatc ctgacttttc aagacctaat agaaagtata cctggaatga tgttggacag
      721 ctagtggaaa aattaaaaaa ggaatggaat gttatttgta ttactgatgt tgtctacaat
      781 catactgctg ctaatagtaa atggatccag gaacatccag aatgtgccta taatcttgtg
      841 aattctccac acttaaaacc tgcctgggtc ttagacagag cactttggcg tttctcctgt
      901 gatgttgcag aagggaaata caaagaaaag ggaatacctg ctttgattga aaatgatcac
      961 catatgaatt ccatccgaaa aataatttgg gaggatattt ttccaaagct taaactctgg
     1021 gaatttttcc aagtagatgt caacaaagcg gttgagcaat ttagaagact tcttacacaa
     1081 gaaaataggc gagtaaccaa gtctgatcca aaccaacacc ttacgattat tcaagatcct
     1141 gaatacagac ggtttggctg tactgtagat atgaacattg cactaacgac tttcatacca
     1201 catgacaagg ggccagcagc aattgaagaa tgctgtaatt ggtttcataa aagaatggag
     1261 gaattaaatt cagagaagca tcgactcatt aactatcatc aggaacaggc agttaattgc
     1321 cttttgggaa atgtgtttta tgaacgactg gctggccatg gtccaaaact aggacctgtc
     1381 actagaaagc atcctttagt taccaggtat tttactttcc catttgaaga gatagacttc
     1441 tccatggaag aatctatgat tcatctgcca aataaagctt gttttctgat ggcacacaat
     1501 ggatgggtaa tgggagatga tcctcttcga aactttgctg aaccgggttc agaagtttac
     1561 ctaaggagag aacttatttg ctggggagac agtgttaaat tacgctatgg gaataaacca
     1621 gaggactgtc cttatctctg ggcacacatg aaaaaataca ctgaaataac tgcaacttat
     1681 ttccagggag tacgtcttga taactgccac tcaacacctc ttcacgtagc tgagtacatg
     1741 ttggatgctg ctaggaattt gcaacccaat ttatatgtag tagctgaact gttcacagga
     1801 agtgaagatc tggacaatgt ctttgttact agactgggca ttagttcctt aataagagag
     1861 gcaatgagtg catataatag tcatgaagag ggcagattag tttaccgata tggaggagaa
     1921 cctgttggat cctttgttca gccctgtttg aggcctttaa tgccagctat tgcacatgcc
     1981 ctgtttatgg atattacgca tgataatgag tgtcctattg tgcatagatc agcgtatgat
     2041 gctcttccaa gtactacaat tgtttctatg gcatgttgtg ctagtggaag tacaagaggc
     2101 tatgatgaat tagtgcctca tcagatttca gtggtttctg aagaacggtt ttacactaag
     2161 tggaatcctg aagcattgcc ttcaaacaca ggtgaagtta atttccaaag cggcattatt
     2221 gcagccaggt gtgctatcag taaacttcat caggagcttg gagccaaggg ttttattcag
     2281 gtgtatgtgg atcaagttga tgaagacata gtggcagtaa caagacactc acctagcatc
     2341 catcagtctg ttgtggctgt atctagaact gctttcagga atcccaagac ttcattttac
     2401 agcaaggaag tgcctcaaat gtgcatccct ggcaaaattg aagaagtagt tcttgaagct
     2461 agaactattg agagaaacac gaaaccttat aggaaggatg agaattcaat caatggaaca
     2521 ccagatatca cagtagaaat tagagaacat attcagctta atgaaagtaa aattgttaaa
     2581 caagctggag ttgccacaaa agggcccaat gaatatattc aagaaataga atttgaaaac
     2641 ttgtctccag gaagtgttat tatattcaga gttagtcttg atccacatgc acaagtcgct
     2701 gttggaattc ttcgaaatca tctgacacaa ttcagtcctc actttaaatc tggcagccta
     2761 gctgttgaca atgcagatcc tatattaaaa attccttttg cttctcttgc ctccagatta
     2821 actttggctg agctaaatca gatcctttac cgatgtgaat cagaagaaaa ggaagatggt
     2881 ggagggtgct atgacatacc aaactggtca gcccttaaat atgcaggtct tcaaggttta
     2941 atgtctgtat tggcagaaat aagaccaaag aatgacttgg ggcatccttt ttgtaataat
     3001 ttgagatctg gagattggat gattgactat gtcagtaacc ggcttatttc acgatcagga
     3061 actattgctg aagttggtaa atggttgcag gctatgttct tctacctgaa gcagatccca
     3121 cgttacctta tcccatgtta ctttgatgct atattaattg gtgcatatac cactcttctg
     3181 gatacagcat ggaagcagat gtcaagcttt gttcagaatg gttcaacctt tgtgaaacac
     3241 ctttcattgg gttcagttca actgtgtgga gtaggaaaat tcccttccct gccaattctt
     3301 tcacctgccc taatggatgt accttatagg ttaaatgaga tcacaaaaga aaaggagcaa
     3361 tgttgtgttt ctctagctgc aggcttacct catttttctt ctggtatttt ccgctgctgg
     3421 ggaagggata cttttattgc acttagaggt atactgctga ttactggacg ctatgtagaa
     3481 gccaggaata ttattttagc atttgcgggt accctgaggc atggtctcat tcctaatcta
     3541 ctgggtgaag gaatttatgc cagatacaat tgtcgggatg ctgtgtggtg gtggctgcag
     3601 tgtatccagg attactgtaa aatggttcca aatggtctag acattctcaa gtgcccagtt
     3661 tccagaatgt atcctacaga tgattctgct cctttgcctg ctggcacact ggatcagcca
     3721 ttgtttgaag tcatacagga agcaatgcaa aaacacatgc agggcataca gttccgagaa
     3781 aggaatgctg gtccccagat agatcgaaac atgaaggacg aaggttttaa tataactgca
     3841 ggagttgatg aagaaacagg atttgtttat ggaggaaatc gtttcaattg tggcacatgg
     3901 atggataaaa tgggagaaag tgacagagct agaaacagag gaatcccagc cacaccaaga
     3961 gatgggtctg ctgtggaaat tgtgggcctg agtaaatctg ctgttcgctg gttgctggaa
     4021 ttatccaaaa aaaatatttt cccttatcat gaagtcacag taaaaagaca tggaaaggct
     4081 ataaaggtct catatgatga gtggaacaga aaaatacaag acaactttga aaagctattt
     4141 catgtttccg aagacccttc agatttaaat gaaaagcatc caaatctggt tcacaaacgt
     4201 ggcatataca aagatagtta tggagcttca agtccttggt gtgactatca gctcaggcct
     4261 aattttacca tagcaatggt tgtggcccct gagctcttta ctacagaaaa agcatggaaa
     4321 gctttggaga ttgcagaaaa aaaattgctt ggtccccttg gcatgaaaac tttagatcca
     4381 gatgatatgg tttactgtgg aatttatgac aatgcattag acaatgacaa ctacaatctt
     4441 gctaaaggtt tcaattatca ccaaggacct gagtggctgt ggcctattgg gtattttctt
     4501 cgtgcaaaat tatatttttc cagattgatg ggcccggaga ctactgcaaa gactatagtt
     4561 ttggttaaaa atgttctttc ccgacattat gttcatcttg agagatcccc ttggaaagga
     4621 cttccagaac tgaccaatga gaatgcccag tactgtcctt tcagctgtga aacacaagcc
     4681 tggtcaattg ctactattct tgagacactt tatgatttat agtttattac agatattaag
     4741 tatgcaatta cttgtattat aggatgcaag gtcatcatat gtaaatgcct tatatgcaca
     4801 ggctcaagtt gttttaaaaa tctcatttat tataatattg atgctcaatt aggtaagatt
     4861 gtaaaagcat tgattttttt taatgtacag aggtagattt caatttgaat cagaaagaaa
     4921 tatcattacc aatgaaatgt gtttgagttc agtaagaatt attcaaatgc ctagaaatcc
     4981 atagtttgga aaagaaaaat catgtcatct tctatttgta cagaaatgaa aataaaatat
     5041 gaaaataatg aaagaaatga aaagatagct tttaattgtg gtatatataa tcttcagtaa
     5101 caatacatac tgaatacgct gtggttcatt aatattaaca ccacgtacta tagtattctt
     5161 aatacagtgc tcactgcatt taataaatat ttaataaatg atgaatgata gaagtttcca
     5221 tctacaatat atgttcctaa atggagcaca gatgttcaaa ctatgctttc attttttcac
     5281 tgatatatta atttttgtgt aatgaatgcc aacagtatat tttatatgat ttacttatgt
     5341 gaggaaacat gcaaagcatt aggaaattta tttcctaaaa acagttttgt aaaattagta
     5401 ttgagttcta ttgagtatta taagatagct tacattttca aaatggaaat tgtcggtcat
     5461 atttctagaa ctttaaagaa aaaagaatgt tatattagtt ttctaaaact caactatctt
     5521 tagtcatgtt caaaaatcta ttgctagatc atagtagata ctggttttct attaactcaa
     5581 aacctacatt gacaagttta acattgagaa gaatcttaac aaaaatatgg atatgaattc
     5641 agtagatatc ttaaattcaa taaaatcact ggaagttttt catgataact tattttaaga
     5701 tgccttaaaa atcttaaagt cacaaaagga aaaaggtttt taacatttac atgagttaac
     5761 attttttcat agaacttatt tcctagatag aattttttac tgttttttac tgttttctta
     5821 agaaaacagt taaatcatta tgcattcagt tggaagaaag tagtggcaag aattctttca
     5881 ttgctatata atattcagtg gctcatttat acctaataaa ataatggtat tttaaaataa
     5941 tgctactttc aaagtagcat ttttttagtt agtttacagg ttacataccc aaaaccttaa
     6001 ctatgactaa gaaattaaag aagaaaacca gcaaactaaa acttctgggc agcaaaaata
     6061 tataaatgct tcagatgtca aatacccatg cttgaaagct cgtgtaattt actttaagat
     6121 tatctgcctg ctcttcttca aagctgacct tgctttagaa atagttttaa ctagcttagt
     6181 tttctggttt ccaaaactaa aatagattaa atcctacaaa tttaaggaca gttgtgacag
     6241 taatctgacc actatctata aatacattgg acattggttt ccaaatctcc ctttcttctt
     6301 cagttccttc cttgttcaat atataccctt ctctaaactg tgcgggtaaa aggaatgact
     6361 gtccttgaga gaaccattag tttatcaaag gtttatgtag ttttgttgct gtaccctaac
     6421 tttgatattc agggaggtag gaaaggtaac agaaaaccag catatttaat caaagcaaga
     6481 agtaatcgct gacagttaaa tgtgaccaaa aaaattaaaa gttcacaatt tttttaatgt
     6541 agccatttgg ggttatctct agtaaggcag atacccacgt tggtaaattt ttaggatatt
     6601 gtgttgcact agaaaactaa gtggttcata tttctaatga ggaagattaa tgaaagaaca
     6661 ttgttatatt ctgcgtggta tattttaaag tttaagaagg catgttaaac attatttcct
     6721 ctatggtagt taaaatacag aattagattt ttaacaggtg tcatttgact aaacgtttcg
     6781 gtagaatgct tcatacttga gtgatgctgg ataaggtatt gtatttcaac aatggactat
     6841 gccttggttt ttcactaatc aaaatcaaaa ttactcttta acatgataaa tgaatttacc
     6901 agtttagtat gctgtggtat tttaataagt tttcaaagat aattgggaaa acatgagact
     6961 ggtcatattg atgaatattg taacatgtga attgtgatcc atttctgata tgtcttgaac
     7021 tactgtgtct agtgggcaaa tgtcattgtt acctctgtgt gttaagaaaa taaaaatatt
     7081 ttctaaaggt c
//
