LOCUS       NM_014423               9536 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens ALF transcription elongation factor 4 (AFF4), mRNA.
ACCESSION   NM_014423
VERSION     NM_014423.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Zhao,Y., Chen,L., Xiang,Q., Lin,J., Jiang,S. and Li,W.
  TITLE     N6-methyladenosine-modified circCDK14 promotes ossification of the
            ligamentum flavum via epigenetic modulation by targeting AFF4
  JOURNAL   Cell Mol Life Sci 81 (1), 436 (2024)
   PUBMED   39414635
  REMARK    GeneRIF: N6-methyladenosine-modified circCDK14 promotes
            ossification of the ligamentum flavum via epigenetic modulation by
            targeting AFF4.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   Zhu,L., Wang,J., Wu,Z., Chen,S., He,Y., Jiang,Y., Luo,G., Wu,Z.,
            Li,Y., Xie,J., Zou,S. and Zhou,C.
  TITLE     AFF4 regulates osteogenic potential of human periodontal ligament
            stem cells via mTOR-ULK1-autophagy axis
  JOURNAL   Cell Prolif 57 (2), e13546 (2024)
   PUBMED   37731335
  REMARK    GeneRIF: AFF4 regulates osteogenic potential of human periodontal
            ligament stem cells via mTOR-ULK1-autophagy axis.
REFERENCE   3  (bases 1 to 9536)
  AUTHORS   Che,Z., Liu,X., Dai,Q., Fang,K., Guo,C., Yue,J., Fang,H., Xie,P.,
            Luo,Z. and Lin,C.
  TITLE     Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in
            regulating RNA polymerase II transcription elongation
  JOURNAL   J Mol Cell Biol 15 (8) (2024)
   PUBMED   37528066
  REMARK    GeneRIF: Distinct roles of two SEC scaffold proteins, AFF1 and
            AFF4, in regulating RNA polymerase II transcription elongation.
REFERENCE   4  (bases 1 to 9536)
  AUTHORS   Long,Q., Xiang,M., Xiao,L., Wang,J., Guan,X., Liu,J. and Liao,C.
  TITLE     The Biological Significance of AFF4: Promoting Transcription
            Elongation, Osteogenic Differentiation and Tumor Progression
  JOURNAL   Comb Chem High Throughput Screen 27 (10), 1403-1412 (2024)
   PUBMED   37815186
  REMARK    GeneRIF: The Biological Significance of AFF4: Promoting
            Transcription Elongation, Osteogenic Differentiation and Tumor
            Progression.
            Review article
REFERENCE   5  (bases 1 to 9536)
  AUTHORS   Yang,Z.M., Zhang,G., Wei,G., Jing,L.L. and Yu,M.
  TITLE     AFF4 globally affects the release of paused RNA polymerase II in
            HEL cells
  JOURNAL   Yi Chuan 45 (8), 658-668 (2023)
   PUBMED   37609817
  REMARK    GeneRIF: AFF4 globally affects the release of paused RNA polymerase
            II in HEL cells.
REFERENCE   6  (bases 1 to 9536)
  AUTHORS   Yokoyama,A., Lin,M., Naresh,A., Kitabayashi,I. and Cleary,M.L.
  TITLE     A higher-order complex containing AF4 and ENL family proteins with
            P-TEFb facilitates oncogenic and physiologic MLL-dependent
            transcription
  JOURNAL   Cancer Cell 17 (2), 198-212 (2010)
   PUBMED   20153263
REFERENCE   7  (bases 1 to 9536)
  AUTHORS   Niedzielski,M.F., Hopewell,R., Ismail,Z. and Estable,M.C.
  TITLE     MCEF is localized to the nucleus by protein sequences encoded
            within three distinct exons, where it represses HIV-1
            Tat-transactivation of LTR-directed transcription
  JOURNAL   Int J Biol Sci 3 (4), 225-236 (2007)
   PUBMED   17389929
  REMARK    GeneRIF: MCEF has 21 exons & 7 nuclear localization sequence. 3
            protein sequences, coded by 3 exons, mediate nuclear localization.
            Ectopic expression of MCEF repressed HIV-1 LTR-directed RNA
            polymerase II transcription, at the level of Tat-transactivation.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 9536)
  AUTHORS   Estable,M.C., Naghavi,M.H., Kato,H., Xiao,H., Qin,J., Vahlne,A. and
            Roeder,R.G.
  TITLE     MCEF, the newest member of the AF4 family of transcription factors
            involved in leukemia, is a positive transcription elongation
            factor-b-associated protein
  JOURNAL   J Biomed Sci 9 (3), 234-245 (2002)
   PUBMED   12065898
  REMARK    GeneRIF: Data describe the cloning and expression of MCEF, a member
            of the AF4 family of transcription factors involved in acute
            lymphoblastic leukemia. [MCEF]
REFERENCE   9  (bases 1 to 9536)
  AUTHORS   Hillman,M.A. and Gecz,J.
  TITLE     Fragile XE-associated familial mental retardation protein 2 (FMR2)
            acts as a potent transcription activator
  JOURNAL   J Hum Genet 46 (5), 251-259 (2001)
   PUBMED   11355014
REFERENCE   10 (bases 1 to 9536)
  AUTHORS   Taki,T., Kano,H., Taniwaki,M., Sako,M., Yanagisawa,M. and
            Hayashi,Y.
  TITLE     AF5q31, a newly identified AF4-related gene, is fused to MLL in
            infant acute lymphoblastic leukemia with ins(5;11)(q31;q13q23)
  JOURNAL   Proc Natl Acad Sci U S A 96 (25), 14535-14540 (1999)
   PUBMED   10588740
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA934874.1, BC100287.1,
            AF197927.1 and AC004500.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_014423.3.
            
            Summary: The protein encoded by this gene belongs to the AF4 family
            of transcription factors involved in leukemia. It is a component of
            the positive transcription elongation factor b (P-TEFb) complex. A
            chromosomal translocation involving this gene and MLL gene on
            chromosome 11 is found in infant acute lymphoblastic leukemia with
            ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC137226.1, AF213987.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265343.10/ ENSP00000265343.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                DA934874.1         29-48
            21-1467             BC100287.1         1-1447
            1468-4278           AF197927.1         1425-4235
            4279-9536           AC004500.1         23305-28562
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.1"
     gene            1..9536
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="ALF transcription elongation factor 4"
                     /db_xref="GeneID:27125"
                     /db_xref="HGNC:HGNC:17869"
                     /db_xref="MIM:604417"
     exon            1..376
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            377..503
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..3872
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="ALL1-fused gene from chromosome 5q31 protein; major
                     CDK9 elongation factor-associated protein"
                     /codon_start=1
                     /product="AF4/FMR2 family member 4"
                     /protein_id="NP_055238.1"
                     /db_xref="CCDS:CCDS4164.1"
                     /db_xref="GeneID:27125"
                     /db_xref="HGNC:HGNC:17869"
                     /db_xref="MIM:604417"
                     /translation="MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSK
                     EDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGS
                     HQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSG
                     SSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDA
                     NWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYS
                     SQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAI
                     HTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDS
                     DGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSD
                     SEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGRE
                     QGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQ
                     PKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPT
                     AEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVE
                     EEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGE
                     KKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNR
                     ESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSK
                     NSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYS
                     ADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSE
                     TVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTE
                     HLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQK
                     IHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVR
                     YTRQGLHWLRQDAKLIS"
     exon            504..1298
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1343
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1430
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1467
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1513
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1568
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1606
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1769
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..2687
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2688..2776
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..3017
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3112
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3176
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3313
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3314..3385
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..3479
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3480..3523
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3524..3744
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     exon            3745..9536
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9390..9395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="hexamer: ATTAAA"
     polyA_site      9414
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
     regulatory      9506..9511
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="hexamer: AATAAA"
     regulatory      9531..9536
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="hexamer: ATTAAA"
     polyA_site      9531
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="major polyA site"
     polyA_site      9536
                     /gene="AFF4"
                     /gene_synonym="AF5Q31; CHOPS; MCEF"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtcaca gccctggctg caggagtcca gctgccgtcg ccgccgccag cggacggggc
       61 ggggatcctg gtgccaggtc ttcgttaacc cgcccccgcc gcctcagtca gctgtcccag
      121 cccgggcccg accgtcatgg agcggtgcgg ggcctacgcc tgttccgtgt gaggcgcatc
      181 gctgccgccg ccgccgacgc cgccttcgtt tgtcgaggcc tccggaaccc ccgccgcctc
      241 ccggagcccc tcgcccttgg cagcctgtca ccgccccctg ggcgggctcg aatgcgcgtc
      301 cggtgaaggt gcaggcccgg cgccgccact gccgcagcca ggagatggtt cgggcctagc
      361 ggagccggga ctggagcaac atgaaccgtg aagaccggaa tgtgctgcgt atgaaagaac
      421 gggaaaggcg gaatcaggaa attcagcagg gcgaagacgc cttcccacct agctctcctc
      481 tctttgcaga gccatacaaa gttactagca aagaagataa gttatcaagt cgtattcaga
      541 gtatgcttgg aaactacgat gaaatgaagg atttcatagg agacagatct ataccaaagc
      601 ttgttgcaat tcccaagcct acagtaccac catcagcaga tgaaaaatct aacccaaatt
      661 tctttgaaca gagacatgga ggctctcatc agagtagcaa atggactcca gtaggacccg
      721 cacccagcac ttctcagtct cagaaacggt cctcaggctt acagagtgga catagtagcc
      781 agcggaccag cgcaggtagc agtagtggca ctaacagtag tggtcagagg cacgaccgtg
      841 agtcatataa caatagtggg agcagtagcc ggaaaaaagg ccagcatgga tcagaacact
      901 ccaaatcacg ttcttccagc cctggaaaac cccaggctgt ttcttcatta aactctagtc
      961 attccaggtc tcatgggaat gatcaccata gcaaggaaca tcaacgctcc aaatcacctc
     1021 gggaccctga tgcaaactgg gattctcctt cccgtgtacc tttttcaagt gggcagcact
     1081 caactcaatc tttcccaccc tcattgatgt caaagtccaa ttcaatgtta cagaaaccca
     1141 ctgcctatgt gcggcccatg gacggacagg agtccatgga accaaagctg tcctctgagc
     1201 actacagcag ccaatcccat ggcaacagca tgactgagct gaagcccagc agcaaagcac
     1261 atctcaccaa gctgaaaata ccttcccaac cactggatgc atcagcttct ggtgatgtga
     1321 gctgtgtgga tgaaatccta aaagagatga cgcattcatg gcctccccct ctaacggcta
     1381 ttcatacacc atgcaaaaca gaaccttcca aatttccttt tccaactaag gagtctcagc
     1441 agtccaattt tggcactgga gaacaaaaaa gatataatcc ttctaaaact tcaaatgggc
     1501 accagtctaa atctatgtta aaagatgact taaaactaag cagcagtgaa gacagtgatg
     1561 gggaacagga ttgtgataag acaatgccga ggagtacacc aggaagtaac tctgaacctt
     1621 cacaccataa tagtgaagga gcagataact ccagggatga ttctagtagc cacagtggat
     1681 ctgaaagcag ctctggatct gactcagaga gtgaaagtag ttccagtgac agtgaggcaa
     1741 atgagccatc ccagagtgca tctcccgagc ctgaaccccc gccaacaaac aaatggcaac
     1801 ttgataattg gctgaataaa gtgaacccac ataaagtgtc acccgcctct tcagtggaca
     1861 gtaacatccc atcatctcaa ggctacaaaa aggaaggccg agagcagggc actgggaata
     1921 gctacactga tacaagtgga cctaaagaaa cgagttccgc tactccggga cgagactcca
     1981 aaaccatcca aaagggatca gaaagtgggc gtgggaggca gaaatctcct gcacagagtg
     2041 acagcacaac acagagaaga actgtaggca aaaaacaacc caaaaaggct gagaaggcag
     2101 ctgctgaaga gcctcgtgga ggcctgaaga tagaaagtga aacccctgta gacttggcta
     2161 gcagcatgcc ctccagcaga cacaaagcag ccaccaaagg ctcaaggaaa cccaatataa
     2221 agaaggagtc taagtcttcc cctcgaccta cagcagagaa aaagaaatat aagtcaacaa
     2281 gtaaatcttc ccagaaatca agggaaatca tagaaacaga tacctcatcc tcagattcag
     2341 atgaaagtga gagccttcct ccttcctcac aaactcctaa gtaccccgag agcaatagga
     2401 ctcctgttaa accctcctca gtggaggaag aagatagctt ttttcggcaa cgaatgttct
     2461 ctcctatgga agagaaggaa cttctttcac ccctcagtga gcctgatgac aggtacccac
     2521 ttattgtgaa gattgacctg aatcttttga ctagaatacc aggaaagcct tacaaagaaa
     2581 cagagccgcc caagggggaa aagaaaaatg tgccagaaaa gcacacgaga gaggctcaga
     2641 aacaagcctc agaaaaagtt tccaacaaag gcaagaggaa gcataagaat gaagatgata
     2701 accgagccag tgagagcaag aaacccaaaa cggaggacaa gaattcagca ggccataagc
     2761 catccagcaa cagagagtca tctaagcaga gtgctgcaaa agaaaaggat ttgttgcctt
     2821 ctcccgctgg gcctgttcct tcaaaagatc caaaaacaga gcatggctct cggaagagga
     2881 ctattagtca gtcttcttcc ttaaagtcaa gcagtaacag caacaaggag acgagtggca
     2941 gcagcaaaaa cagttcctcc acatcaaagc agaagaagac cgaagggaag acttccagta
     3001 gctccaagga ggttaaggaa aaggctccaa gtagctcctc taactgtcct ccatctgcac
     3061 caactcttga ttcttctaag cctcggagaa caaagcttgt ctttgatgac agaaattatt
     3121 cagcagacca ttatttacaa gaagcaaaaa agctaaagca caatgcagat gcattgtctg
     3181 ataggtttga gaaagctgta tactatcttg atgctgtggt atctttcatt gaatgtggga
     3241 atgcattaga gaagaatgct caggaatcca aatccccatt ccctatgtat tcagagacgg
     3301 tggatctcat caaatacact atgaagctaa agaattactt ggcaccagat gctacagctg
     3361 cagataaacg actcacagta ctttgcctgc gatgcgagtc tttgctgtac ctgaggctgt
     3421 tcaaactgaa gaaggaaaat gctctgaagt actcaaagac actgacagag cacctgaaga
     3481 attcttataa taattctcaa gcaccatcgc ctggcttggg aagcaaagct gtggggatgc
     3541 cttcccctgt ttctccaaag ctgtcaccag gcaattcagg aaattattca tctggggcca
     3601 gtagtgcttc tgcaagtggt tcttcagtga ccattccaca gaagatccac cagatggcag
     3661 ccagctatgt tcaggtcaca tccaacttcc tctatgccac cgaaatttgg gaccaagctg
     3721 aacagctttc caaagagcaa aaagaattct ttgctgaact ggataaagta atgggccctc
     3781 tcatctttaa tgcaagcatc atgacagatc tagttcgtta tacccggcag ggactgcact
     3841 ggcttcgcca ggatgccaag ttgatatctt gaactgaaca cattctcgtt gcctctgatt
     3901 ttctccacaa cactgtgtca catcacgaag gaaaactgcc ataacatacc acctagtcga
     3961 cactaagaat gaggaatagt tttctcctcg ttggttcatg tgttgttgtt tttgataatc
     4021 caaagcgatc atgtcagttg gccctttaat atttccaatg tgaaagatta tttaaatgct
     4081 tttaaatctg cagcacattg ataagatggt ttccgtgagc tatgataaga ttgaaattcc
     4141 agttgcaatt cataactaat gaattgaaat tttatttatt ttaatttttt taagttccca
     4201 gattggggct agtttaagac ttagttattt ctgcctataa atttactctg ttgaatattt
     4261 agcataaatt taatgtagac atttttattt atataccttg ggagctttat tacctaatat
     4321 ctttttgtgg tagctgttct aagagtaaga attggttcgt ctaaaataag gacatattct
     4381 ttctacacct gctacctgac ctgatatttg aaatcttaac tccaatttta ttattgtgga
     4441 atttacagtt agtattaagg atattcttct acacatgaaa aaatcactta aatatgagaa
     4501 aatggcattc tacatgtatg tactatgaaa tgtgtctgat ttggtatgca ttatttcaaa
     4561 aactgtgaat gtgtgttaat aacactacag caatcacaca gtgactatag cttgcattta
     4621 atgcattaca aaagagggag gacaaacttt ctagaagaag catttcatgt tgaagggttt
     4681 cataaaagga caatcctgaa cgttacggat tttattaaaa ccatatgtta ttttttgttg
     4741 ctaatcattt gacacttaat cgaaacagaa taagttactg tggaaaaaag gatgatccaa
     4801 ctcctgagga ttgccgcctg tgtgtcttta attgattatc tttgatagtt agagttattt
     4861 ttcagatttt tccccctcat actggattgt gatgctctga tatgagcatg caatgactga
     4921 tacagaaata ggctggagct tcggtgtgtg cttttgctat acagcttaca gttctaatgc
     4981 ctgatgtaga gatgatgaat ttctcctttt taaacctatc ttctgtttac tccttttgca
     5041 ttatgaaggc cctcttcaat gatgtatgtt tctgatggaa tcagaaaatt tcacactaga
     5101 aaagtgtacc taacttgatt cagtgtgagc tcttgggaca gggagtggag ccaggggtag
     5161 agcttaaaga agctggctct gtaagagttg tatatcaaag taccttttgc tacaaggtgc
     5221 atatagaagt ctaagataag aaatgtaccc tctatacttt atttttcatg cccctatcat
     5281 gtttggtgtg ccttaaatct tacctttaaa tgaagactct atggattgtt tacaaatgaa
     5341 gttttacact aggaccagat cgtccataca tgattggtct tctacaataa taatgttcaa
     5401 ttctttcatt ttaaatcttt ctttagggtc atataaagat tagttctttc agaaaagcta
     5461 ttgctttggt acatgactta gtctaaccag ttgggtaatg ctgctttgaa aaatagtttg
     5521 tccctctccc cattccaaaa atgaatccat tcagtcagaa aaaaagatta tcctactatt
     5581 tgtagatgat tgctaaggct tgaaatagga aaggggaaag aagggataga aaagtttcaa
     5641 gtccagtagt gcagtgaatc ctttgtgttt gagacttttt tacacttatt tctgaagtta
     5701 gtgaactttc attgtccctg tggcctcata tttatagagc ttcttaactg ttttgtacct
     5761 cagattgttt ttagatgtca ttgaagattg agatgtacaa attatgttga atttaacctg
     5821 tttctcttgg agaaattgcc tctgatatct ttctaactta atcttagtgt gttctaggaa
     5881 cagttttcca taagaggaca acttctcaat gatcatgata gttatttagt aagaaataat
     5941 ggttgttaat caaatacatg ttatgtcttg tccttctctg atttttctga cttggattcc
     6001 ttaatttgca gtgatgtctt agaaatggaa aaagtatcct ctgggctttc agactttcag
     6061 caacaacagc cagtgggata cctctgagtt cttgtgttct agtctcttgt atgtcctgaa
     6121 aggcagcagg agcatttttg gggtcacact gatattttgt taaagcagag tttcctgcat
     6181 ggtttatggt gctgctctaa tattttctct ttaaaactgt gtcaattttt ggagaatgaa
     6241 tttttcaacc ttttcattgt ggaggatttt tcccacaagg tagtaatggg caatggatca
     6301 aaatcctatc ataaatgatt gaattttcaa tgtcatagtg tgtggaaata ctactccctt
     6361 tcctccatgt ctatcttctt agatgttctg tctttgtcat catctgctcc agcacatact
     6421 ttggactcct ttacaggtga ccttcccccc tccctcctca ataggtgtcc tcagtgtttt
     6481 ctaagcattt aaaactgaat ttgacaagtg gctaaaaact tagtctgaga cttgtactta
     6541 gtatatggtt aattatagaa acaacaacta ttttaaacag ttactctgtt gactgctctt
     6601 tatgactaca cttgtatgcc aggaggcagc agcctataca ccattagact ctttacaagc
     6661 aaccaacgaa ccaattgcag tggttgcagt tgtcacagta gtcttgcctg ggccagactg
     6721 ggctgggctg tgggaagcat tccccttcag gggagtttcc acagctcagc ccaggctcca
     6781 gtctggctag agagcatcca gcccattcct tgcctcagtg ccaccttttt ctgggcaagt
     6841 agccaagaag tgactttata tgcagtgagt acttcagata aacttacagc tacagctgcc
     6901 gaagagtgga gaaggttgac caccatttat ttaattctct agtgtaaaaa caattgagag
     6961 cagtgtgcgt gagcccacag acacctgtgt ttctgggagc tctggcagtg gcggattcat
     7021 aggcacttgg gctgcacttt gaatgacaca cttggcttta ttagattcac tagtttttaa
     7081 aaaattgttg ttcgtttctt ttcattaaag gtttaatcag acagatcaga cagcataatt
     7141 ttgtatttaa tgacagaaac gttggtacat ttcttcatga atgagcttgc attctgaagc
     7201 aagagcctac aaaaggcact tgttataaat gaaagttctg gctctagagg ccagtactct
     7261 ggagtttcag agcagccagt gattgttcca gtcagtgatg cctagttata tagaggagga
     7321 gtacactgtg cactcttcta ggtgtaaggg tatgcaactt tggatcttaa aattctgtac
     7381 acatacacac tttatatata tgtatgtatg tatgaaaaca tgaaattagt ttgtcaaata
     7441 tgtgtgtgtt tagtatttta gcttagtgca actatttcca cattatttat taaattgatc
     7501 taagacactt tcttgttgac accttgaata ttaatgttca agggtgcaat gtgtattcct
     7561 ttagattgtt aaagcttaat tactatgatt tgtagtaaat taacttttaa aatgtatttg
     7621 agcccttctg tagtgtcgta gggctcttac agggtgggaa agattttaat tttccagttg
     7681 ctaattgaac agtatggcct cattatatat tttgatttat aggagtttgt gtctgggtcc
     7741 caggaacatc tactggggat gactgttagg cagcttgtga tgatgttttt taaaaaacct
     7801 aagtaacttg gggagacaga gcatttcaaa cccatataga cacctatcat acctgtatat
     7861 cccctaatac atggaacata aatttcattt cagctttatg cattaatgta ttgaagaggc
     7921 aggctttttt tccagtaata atactgtaca tctaaggttg ctattcagta aaactaatag
     7981 aagagataat tattgaatac cttggaatat gtagtgagaa gaggccaaat ttttcatgtt
     8041 tgtgagcatg tctttcagat actcgctaat ggagaaattc actttagacc tctagacccc
     8101 aaaaccaagt tagcaagtga caaaaagtaa attattaata gtctgtaaag tggatgctgg
     8161 tcttacaaag ctcctattca catgaaccct atggttaatt agaaagaact tgctgtctcc
     8221 agaaaaggga atggaaataa taactatgta tcttcttttc atgtaatttg gtcacagaat
     8281 aaggtattca agttaagtct aatccaagtc ttagagatct ttaagtatcc taaaatagca
     8341 gcctaagtag tcatcatgta aagaagttga ctttcaggaa tgtcagcatt gacctctcct
     8401 tgccactgtt actcagctaa acataacttg ccattcttaa aaataaaaaa gctcccaccc
     8461 agaaactcta gtgatactca gttttgacct cttactgctt ccatgtggga cttcttttca
     8521 gcttttaacc attggaattt tctacctgct atgttcctct ctggaaaata tcaatttgga
     8581 aatgctgcct ttcaaatcaa tgccatgatc atccatattg ttgagtttgt tgttggtgtt
     8641 ccagaagtgt ccccatttta caactttatg cacacacctg gcaaatcatt aacatgagca
     8701 ttcaacagta ctgggatggg tggggtgggg gtagggggct caccaacaga actggccagt
     8761 tgacattgcc tgaaaaggca ccacctttaa gggccagttg tggggttggt aagcacagtt
     8821 atgaaaattt ctatctagcc tccgttttga atggttttaa gaaattttgt atatggagga
     8881 tggaaatgct tatatattta ttgaaaatcc ttttatttga aattaaaatt atttggtata
     8941 taattgtttg atatttgggg agggttttgg aggggagata atgtcactga taaattaaaa
     9001 gcctgttgat tttgcagaaa ggtactgtta gtgactgata aggatgcctt tgttttgtac
     9061 atgaaccaaa tctttgtttt acatttcttt tatccaaaat atttaggcat ctgacatttt
     9121 caaccagaat ttcaattata tactgtgatt tttatttgtt gcaatacatt acaatttcag
     9181 aggtactttg gggctcaaaa gctaggattt ctaagtcagt atgtgcattt cacacgataa
     9241 agctgttaat ggtgagcaaa gtattatata ctaactagat ttttccacat ctgtatagta
     9301 tattatatgt attttgtggt attgagagta tagaaagttt agagtgtcaa acatgcgttt
     9361 aatgtgtatt tttaaacaaa tttatggcaa ttaaaatatt tggagacaag ggtatcacat
     9421 ttcaatttgt aaagatcttt ttaactctac tgtgtcatta agatgctttg tacaatgcca
     9481 aaccatagtt ggagaaacta agtttaataa atatcaaata gattttctgt attaaa
//
