LOCUS       NM_001386135           10070 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens ALF transcription elongation factor 3 (AFF3),
            transcript variant 3, mRNA.
ACCESSION   NM_001386135
VERSION     NM_001386135.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Fan,A., Li,Y., Zhang,Y., Meng,W., Pan,W., Chen,M., Ma,Z. and
            Chen,W.
  TITLE     Loss of AR-regulated AFF3 contributes to prostate cancer
            progression and reduces ferroptosis sensitivity by downregulating
            ACSL4 based on single-cell sequencing analysis
  JOURNAL   Apoptosis 29 (9-10), 1679-1695 (2024)
   PUBMED   38478171
  REMARK    GeneRIF: Loss of AR-regulated AFF3 contributes to prostate cancer
            progression and reduces ferroptosis sensitivity by downregulating
            ACSL4 based on single-cell sequencing analysis.
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Wang,Q., Chen,S., Wang,G., Zhang,T. and Gao,Y.
  TITLE     Integrated mendelian randomization analyses highlight AFF3 as a
            novel eQTL-mediated susceptibility gene in renal cancer and its
            potential mechanisms
  JOURNAL   BMC Cancer 24 (1), 739 (2024)
   PUBMED   38886730
  REMARK    GeneRIF: Integrated mendelian randomization analyses highlight AFF3
            as a novel eQTL-mediated susceptibility gene in renal cancer and
            its potential mechanisms.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10070)
  AUTHORS   Bassani,S., Chrast,J., Ambrosini,G., Voisin,N., Schutz,F.,
            Brusco,A., Sirchia,F., Turban,L., Schubert,S., Abou Jamra,R.,
            Schlump,J.U., DeMille,D., Bayrak-Toydemir,P., Nelson,G.R.,
            Wong,K.N., Duncan,L., Mosera,M., Gilissen,C., Vissers,L.E.L.M.,
            Pfundt,R., Kersseboom,R., Yttervik,H., Hansen,G.A.M., Smeland,M.F.,
            Butler,K.M., Lyons,M.J., Carvalho,C.M.B., Zhang,C., Lupski,J.R.,
            Potocki,L., Flores-Gallegos,L., Morales-Toquero,R., Petit,F.,
            Yalcin,B., Tuttle,A., Elloumi,H.Z., McCormick,L., Kukolich,M.,
            Klaas,O., Horvath,J., Scala,M., Iacomino,M., Operto,F., Zara,F.,
            Writzl,K., Maver,A., Haanpaa,M.K., Pohjola,P., Arikka,H.,
            Kievit,A.J.A., Calandrini,C., Iseli,C., Guex,N. and Reymond,A.
  TITLE     Variant-specific pathophysiological mechanisms of AFF3 differently
            influence transcriptome profiles
  JOURNAL   Genome Med 16 (1), 72 (2024)
   PUBMED   38811945
  REMARK    GeneRIF: Variant-specific pathophysiological mechanisms of AFF3
            differently influence transcriptome profiles.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10070)
  AUTHORS   Inoue,Y., Tsuchida,N., Okamoto,N., Shuichi,S., Ohashi,K.,
            Saitoh,S., Ogawa,A., Hamada,K., Sakamoto,M., Miyake,N.,
            Hamanaka,K., Fujita,A., Koshimizu,E., Miyatake,S., Mizuguchi,T.,
            Ogata,K., Uchiyama,Y. and Matsumoto,N.
  TITLE     Three KINSSHIP syndrome patients with mosaic and germline AFF3
            variants
  JOURNAL   Clin Genet 103 (5), 590-595 (2023)
   PUBMED   36576140
  REMARK    GeneRIF: Three KINSSHIP syndrome patients with mosaic and germline
            AFF3 variants.
REFERENCE   5  (bases 1 to 10070)
  AUTHORS   Khan,H., Koh,G., Chong,A.E.Q., Zahid,M., Hussain,S., Ali,H.,
            Ahmad,W. and Xue,S.
  TITLE     A novel variant in AFF3 underlying isolated syndactyly
  JOURNAL   Clin Genet 103 (3), 341-345 (2023)
   PUBMED   36273379
  REMARK    GeneRIF: A novel variant in AFF3 underlying isolated syndactyly.
REFERENCE   6  (bases 1 to 10070)
  AUTHORS   To,M.D., Faseruk,S.A., Gokgoz,N., Pinnaduwage,D., Done,S.J. and
            Andrulis,I.L.
  TITLE     LAF-4 is aberrantly expressed in human breast cancer
  JOURNAL   Int J Cancer 115 (4), 568-574 (2005)
   PUBMED   15704140
  REMARK    GeneRIF: LAF-4 may be a proto-oncogene that is transcriptionally
            activated in some cases of breast cancer.
REFERENCE   7  (bases 1 to 10070)
  AUTHORS   Hiwatari,M., Taki,T., Taketani,T., Taniwaki,M., Sugita,K.,
            Okuya,M., Eguchi,M., Ida,K. and Hayashi,Y.
  TITLE     Fusion of an AF4-related gene, LAF4, to MLL in childhood acute
            lymphoblastic leukemia with t(2;11)(q11;q23)
  JOURNAL   Oncogene 22 (18), 2851-2855 (2003)
   PUBMED   12743608
  REMARK    GeneRIF: This gene is fused to MLL in childhood acute lymphoblastic
            leukemia with t(2;11)(q11;q23).
REFERENCE   8  (bases 1 to 10070)
  AUTHORS   von Bergh,A.R., Beverloo,H.B., Rombout,P., van Wering,E.R., van
            Weel,M.H., Beverstock,G.C., Kluin,P.M., Slater,R.M. and
            Schuuring,E.
  TITLE     LAF4, an AF4-related gene, is fused to MLL in infant acute
            lymphoblastic leukemia
  JOURNAL   Genes Chromosomes Cancer 35 (1), 92-96 (2002)
   PUBMED   12203795
  REMARK    GeneRIF: The LAF4 gene mapped on chromosome region 2q11.2-q12 has
            been identified as a fusion partner of the MLL gene in a case of
            infant acute lymphoblastic leukemia.
REFERENCE   9  (bases 1 to 10070)
  AUTHORS   Liao,X., Ma,C., Trask,B., Massa,H., Gilbert,D.J., Staudt,L.M.,
            Jenkins,N.A. and Copeland,N.G.
  TITLE     LAF4 maps to mouse chromosome 1 and human chromosome 2q11.2-q12
  JOURNAL   Mamm Genome 7 (6), 467-468 (1996)
   PUBMED   8662235
REFERENCE   10 (bases 1 to 10070)
  AUTHORS   Ma,C. and Staudt,L.M.
  TITLE     LAF-4 encodes a lymphoid nuclear protein with transactivation
            potential that is homologous to AF-4, the gene fused to MLL in
            t(4;11) leukemias
  JOURNAL   Blood 87 (2), 734-745 (1996)
   PUBMED   8555498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092667.3, AC010736.13,
            KF456685.1, AC073118.4, AC093373.2 and AC018690.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a tissue-restricted nuclear
            transcriptional activator that is preferentially expressed in
            lymphoid tissue. Isolation of this protein initially defined a
            highly conserved LAF4/MLLT2 gene family of nuclear transcription
            factors that may function in lymphoid development and oncogenesis.
            In some ALL patients, this gene has been found fused to the gene
            for MLL. Multiple alternatively spliced transcript variants that
            encode different proteins have been found for this gene. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: BC136579.1, U34360.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000672756.2/ ENSP00000500419.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               AC092667.3         170155-170261       c
            108-190             AC092667.3         156895-156977       c
            191-270             AC092667.3         133175-133254       c
            271-387             AC092667.3         132073-132189       c
            388-508             AC092667.3         36483-36603         c
            509-821             AC092667.3         34819-35131         c
            822-1207            AC092667.3         34303-34688         c
            1208-1255           AC010736.13        107314-107361       c
            1256-1336           AC010736.13        22058-22138         c
            1337-1373           AC010736.13        13941-13977         c
            1374-1425           KF456685.1         176-227             c
            1426-1477           AC073118.4         47855-47906         c
            1478-1518           AC073118.4         24943-24983         c
            1519-1705           AC093373.2         124-310             c
            1706-2800           AC018690.5         223001-224095       c
            2801-2925           AC018690.5         216960-217084       c
            2926-3127           AC018690.5         212604-212805       c
            3128-3252           AC018690.5         208133-208257       c
            3253-3316           AC018690.5         198658-198721       c
            3317-3453           AC018690.5         195293-195429       c
            3454-3525           AC018690.5         190171-190242       c
            3526-3619           AC018690.5         188681-188774       c
            3620-3669           AC018690.5         184489-184538       c
            3670-3893           AC018690.5         184117-184340       c
            3894-10070          AC018690.5         175225-181401       c
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..10070
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="ALF transcription elongation factor 3"
                     /db_xref="GeneID:3899"
                     /db_xref="HGNC:HGNC:6473"
                     /db_xref="MIM:601464"
     exon            1..107
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            108..190
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            191..270
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            271..387
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     CDS             335..4015
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="isoform 1 is encoded by transcript variant 3;
                     MLLT2-related protein; lymphoid nuclear protein related to
                     AF4; lymphoid nuclear protein 4; protein LAF-4; AF4/FMR2
                     family member 3"
                     /codon_start=1
                     /product="AF4/FMR2 family member 3 isoform 1"
                     /protein_id="NP_001373064.1"
                     /db_xref="CCDS:CCDS42723.1"
                     /db_xref="GeneID:3899"
                     /db_xref="HGNC:HGNC:6473"
                     /db_xref="MIM:601464"
                     /translation="MDSFDLALLQEWDLESLCVYEPDRNALRRKERERRNQETQQDDG
                     TFNSSYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDFLTDRSNQSHLVGVPKPGVPQ
                     TPVNKIDEHFVADSRAQNQPSSICSTTTSTPAAVPVQQSKRGTMGWQKAGHPPSDGQQ
                     RATQQGSLRTLLGDGVGRQQPRAKQVCNVEVGLQTQERPPAMAAKHSSSGHCVQNFPP
                     SLASKPSLVQQKPTAYVRPMDGQDQAPDESPKLKSSSETSVHCTSYRGVPASKPEPAR
                     AKAKLSKFSIPKQGEESRSGETNSCVEEIIREMTWLPPLSAIQAPGKVEPTKFPFPNK
                     DSQLVSSGHNNPKKGDAEPESPDNGTSNTSMLEDDLKLSSDEEENEQQAAQRTALRAL
                     SDSAVVQQPNCRTSVPSSKGSSSSSSSGSSSSSSDSESSSGSDSETESSSSESEGSKP
                     PHFSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGSESNQYYNPVKEDVQDCG
                     KVPDVCQPSLREKEIKSTCKEEQRPRTANKAPGSKGVKQKSPPAAVAVAVSAAAPPPA
                     VPCAPAENAPAPARRSAGKKPTRRTERTSAGDGANCHRPEEPAAADALGTSVVVPPEP
                     TKTRPCGNNRASHRKELRSSVTCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLE
                     SEQEEYPLSKAQTVAASASSGNDQRLKEAAANGGSGPRAPVGSINARTTSDIAKELEE
                     QFYTLVPFGRNELLSPLKDSDEIRSLWVKIDLTLLSRIPEHLPQEPGVLSAPATKDSE
                     SAPPSHTSDTPAEKALPKSKRKRKCDNEDDYREIKKSQGEKDSSSRLATSTSNTLSAN
                     HCNMNINSVAIPINKNEKMLRSPISPLSDASKHKYTSEDLTSSSRPNGNSLFTSASSS
                     KKPKADSQLQPHGGDLTKAAHNNSENIPLHKSRPQTKPWSPGSNGHRDCKRQKLVFDD
                     MPRSADYFMQEAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYT
                     MYSETVELIRYAMRLKTHSGPNATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSK
                     ALIDYFKNSSKAAQAPSPWGASGKSTGTPSPMSPNPSPASSVGSQGSLSNASALSPST
                     IVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFNDLDLLMGPVTLHSS
                     MEHLVQYSQQGLHWLRNSAHLS"
     exon            388..508
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            509..821
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            822..1207
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1255
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1336
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1373
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1425
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1477
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..1518
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1705
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..2800
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2925
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3127
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3128..3252
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3253..3316
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3453
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3525
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3619
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3669
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3670..3893
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     exon            3894..10070
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8215..8220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="hexamer: ATTAAA"
     polyA_site      8239
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="major polyA site"
     regulatory      9603..9608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="hexamer: TATAAA"
     polyA_site      9625
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
     regulatory      10053..10058
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
                     /note="hexamer: AATAAT"
     polyA_site      10070
                     /gene="AFF3"
                     /gene_synonym="KINS; LAF4; MLLT2-like"
ORIGIN      
        1 gccattcaga aagctcgcca gcgagaagag agctccacgc tcagcctcag aagaaagacg
       61 ttcaacgact tactggggag agaaagaaaa ggaacgggag ctgagagaaa gtttcacaca
      121 tatgatttag accttacgat cagccattgt ttacatcggg aagcatcaat ccggagtaac
      181 tggcaagcag ctgatcacct gcctttccag accggagacg ggcggcaagt ttgaagtcga
      241 cacccagagg ccagtttgtc ccccttcccg cgcgcgggcg gcccgagcct cggcggcggc
      301 ggtagcggcg gcggcgacgc tgacacctcc caccatggac agcttcgact tagccctgct
      361 ccaggaatgg gacctcgagt cactgtgtgt ctatgaacca gatagaaatg cattacggag
      421 gaaagaacga gaaagaagaa atcaagaaac tcaacaggat gatggcacgt ttaattctag
      481 ttactctctc ttcagtgagc cctacaagac taacaagggg gatgaactct ccaaccggat
      541 ccagaacact ttaggcaatt atgatgaaat gaaagacttt ttaactgata gatccaatca
      601 gagtcatctc gttggagttc ccaaacctgg ggttcctcag actcctgtga acaagatcga
      661 tgaacatttt gttgcagatt caagagccca gaaccagccc tcgtctatct gtagcactac
      721 aacttccaca ccagcagctg tccccgtgca gcagagtaag agaggcacta tgggctggca
      781 gaaggctggg cacccaccct ctgacggcca acagagagca acacaacagg gctctctcag
      841 gaccttgctt ggagatggtg ttggcagaca gcagcctcgg gccaaacaag tgtgcaatgt
      901 ggaggtgggc cttcagaccc aggagaggcc acctgccatg gcggccaagc acagcagcag
      961 cggacactgt gttcagaact ttcctccatc cctagcttca aaacccagcc tggtccagca
     1021 gaaaccgacc gcgtatgtga ggccaatgga cggccaagat caggcccctg atgagtctcc
     1081 taagctgaag tcgtcttcgg aaaccagcgt gcactgcaca tcatacaggg gagtccctgc
     1141 cagcaagccg gagcctgcca gagccaaggc caagctctcc aagttcagca tccccaagca
     1201 gggggaggag agtagatctg gagaaaccaa cagctgtgtt gaagaaataa tccgggagat
     1261 gacctggctt ccaccacttt ctgctattca agcacctggc aaagtggaac caaccaaatt
     1321 tccatttcca aataaggact ctcagcttgt atcctctgga cacaataatc caaagaaagg
     1381 tgatgcagag ccagagagtc cagacaatgg cacatcgaat acatcaatgc tggaagatga
     1441 ccttaagcta agcagtgatg aagaggagaa tgaacagcag gcagctcaga gaacggctct
     1501 ccgcgctctc tctgacagcg ccgtggtcca gcagcccaac tgcagaacct cggtgccttc
     1561 cagcaagggc agcagcagca gcagcagcag cggcagcagc agctcctcca gcgactcaga
     1621 gagcagctcc ggatctgact cggagaccga gagcagctcc agcgagagtg agggcagcaa
     1681 gcccccccac ttctccagcc ccgaggctga accggcatcc tctaacaagt ggcagctgga
     1741 taaatggcta aacaaagtta atccccacaa gcctcctatt ctgatccaaa atgaaagcca
     1801 cgggtcagag agcaatcagt actacaaccc ggtgaaagag gacgtccagg actgtgggaa
     1861 agtccccgac gtttgccagc ccagcctgag agagaaggag atcaagagca cttgcaagga
     1921 ggagcaaagg ccaaggacag ccaacaaggc ccctgggagt aaaggcgtga agcagaagtc
     1981 cccgcccgcg gccgtggccg tggcggtgag cgcagccgcc ccgccacccg cagtgccctg
     2041 tgcgcccgcg gagaacgcgc ccgcgcctgc ccggaggtcc gcgggcaaga agcccaccag
     2101 gcgcaccgag aggacctcag ccggggacgg cgccaactgc caccggcccg aggagcccgc
     2161 ggccgcggac gcgctgggga cgagcgtggt ggtccccccg gagcccacca aaaccaggcc
     2221 ctgtggcaac aacagagcga gccaccgcaa ggagctgcgc tcctccgtga cctgcgagaa
     2281 gcgccgcacg cgggggctaa gcaggatcgt ccccaaatcc aaggagttca ttgagacaga
     2341 gtcgtcatct tcatcctcct cctcggactc cgacctggag tccgagcagg aggagtaccc
     2401 tctgtccaaa gcacagaccg tggctgcctc tgcctcctcc gggaatgatc agaggctgaa
     2461 ggaggccgct gccaacgggg gcagtggtcc tagggcccct gtaggctcca tcaacgccag
     2521 gaccaccagt gacatcgcca aggagctgga ggagcagttc tacacactgg tcccctttgg
     2581 ccggaacgaa cttctctccc ctctaaagga cagtgatgag atcaggtctc tctgggtcaa
     2641 aatcgacctg accctcctgt ccaggatccc agaacacctg ccccaggagc caggggtatt
     2701 gagcgcccct gccaccaagg actctgagag cgcaccgccc agccacacct cggacacacc
     2761 tgcagaaaag gctttgccaa aatccaagag gaaacgcaag tgtgacaacg aagacgacta
     2821 cagggagatc aagaagtccc agggagagaa agacagctct tcaagactgg ccacctccac
     2881 cagtaatact ttgtctgcaa accactgcaa catgaacatc aacagtgtgg caataccaat
     2941 aaataaaaat gaaaaaatgc ttcggtcgcc catctcaccc ctctctgatg catctaaaca
     3001 caaatacacc agcgaggact taacttcttc cagccgacct aatggcaaca gtttgtttac
     3061 ttcagcctct tccagcaaaa agcctaaggc cgacagccag ctgcagcctc acggcggaga
     3121 cctcacgaaa gcagctcaca acaattctga aaacattccc ctccacaagt cacggccgca
     3181 gacgaagccg tggtctccag gctccaacgg ccacagggac tgcaagaggc agaaacttgt
     3241 cttcgatgat atgcctcgca gtgccgatta ttttatgcaa gaagctaaac gaatgaagca
     3301 taaagcagat gcaatggtgg aaaagtttgg aaaggctttg aactatgctg aagcagcatt
     3361 gtcgtttatc gagtgtggaa atgcaatgga acaaggcccc atggaatcca aatctcctta
     3421 tacgatgtat tcagaaacag tagagctcat caggtatgct atgagactaa aaacccactc
     3481 aggccccaat gccacaccag aagacaaaca actggctgca ttatgttacc gatgcctggc
     3541 cctcctgtac tggcggatgt ttcgactcaa aagggaccac gctgtaaagt attcaaaagc
     3601 actaatcgac tatttcaaga actcatctaa agccgcccaa gccccatctc cgtggggggc
     3661 cagtggaaag agcactggaa ccccatcccc catgtctccc aacccctctc ccgccagctc
     3721 cgtggggtct cagggcagcc tctccaacgc cagcgccctg tccccgtcga ccatcgtcag
     3781 catcccacag cgcatccacc agatggcggc caaccacgtc agcatcacca acagcatcct
     3841 gcacagctac gactactggg agatggccga caacctggcc aaggaaaacc gagaattctt
     3901 caacgacctg gatctgctca tggggccggt caccctgcac agcagcatgg agcacctggt
     3961 ccagtactcc caacagggcc tgcactggct gcggaacagc gcccacctgt catagggacc
     4021 tcaccctggg gccagagtgg gctctggtct ccacagatgg ctcaacgttt ttggacactg
     4081 tgctactgaa actcccagcc acagcattta tcagactgcg gtgaacattt cctcagcatt
     4141 gaacaatgat cttttgcagg gcatgtgtgt ttgtatgtgt gggtatataa gaagccgcct
     4201 gtgtatgtgt gtaagataca cagctcttca gaacacacct tctttgtcta gtttccataa
     4261 tcccaggcta gacaaagtga tcagaggttt ctgactagag gaagtacaca cacacacaca
     4321 cacacacaca cacaccgtac actcacacac aatcacagac acacactttc tattccattc
     4381 cagtgctaaa ccgtgacttc ttagaaaatt caaccaaaat ctcatgggtt agttaaccag
     4441 gtgcaataca gcacaccaaa agcttgactg ccagtgaaga tggacctgct cttatactgt
     4501 tgattacttt cagtattaat atactattcc ccaattattt tttgattggt atgtattaat
     4561 gggtaattga ataatgaaaa taagccaggt atttttgtgc cggaagttta ctagattgca
     4621 tgttaccaaa taccctgctc ttcctctaat tcccttcccc catctgactc tattatcaac
     4681 taaatgcatt ccaggcaaac agatggacac tcacagccct tcccagcccc acctccaagt
     4741 agtatgcaaa tggcactgag atttagcaaa aacgcggcgt ctttgaaagt taacagacac
     4801 taaggtagca gtgcctgtgt gttggagctc agttaggagt tgaagttttc tgcacctgtg
     4861 ataacaagcc caccagccac atgcatttct tcttcctgcc actgtgactc ggtggctgta
     4921 gagccacatg gcatttgtca gccaatctgt ttcaagtctt ttctttccac caatagcttt
     4981 cttacagatt ttcttggcta aatgaggcca ttacctagat tgtcccttca gtaggagcct
     5041 gaatccttcg cttaccaggc tgagagctat ttgccatgat aatgctgatt ctctcactcc
     5101 tcatgcagaa tgtgcacctg cagccctttg atgcgttttg atttgggggc gatttttcag
     5161 gctttgatta gcaggctgct tgcaatgcag aagatcacag ttcctttctg gggcagcagc
     5221 aaccagagcc tcttcctgtg tcctgtgtgt ctggtatgtt tacaccttgc tctgagtttt
     5281 tctcttgttt tcttttttta attaagttta cattttagtt tctttgacat cttgtttaca
     5341 gtttttgtct gatgtttttt cttttgtagt gtcttatgcc ctagatttag tcatcttgtt
     5401 aaatatattt ggatatgaaa tcttgacgaa aagaaaataa ctcctgaaaa agagggtcta
     5461 tcttgccaca cgttaaaaac aaaaataatg tccttgtggc ctcttacttg ataaagttgc
     5521 accaggacgg tctgagaaag gcaggcggtg taatatgtgg tgcaatccat tttttcaagg
     5581 tgatgacggc acaaaatact tagactagat gctgatccat aaacattcac tgatattctt
     5641 acaggttggt ttgcagtgtt aacatttttg ttagcctgat ctgtaggtgg gaagtaagat
     5701 catcataatt ccttagctct gagcactgat tcaaaattgt ataaatgact gaacaactta
     5761 gatgatttga aattaattga aaataagaag ggcttgtgga atttgcattt ggctttggcc
     5821 ttcaagcatt tggaaatatt aagagaattt ccattttaca tgtctccaac tggggtgctt
     5881 cttacaaccc tttcactttt tttttgagac gagtttcgct ctgttgccca ggctggagtg
     5941 cagtggcacg atctcggctc actgcatcct ctgcctcctg ggttcaagtg attgtcccgc
     6001 ctcagcctcc caagtagctg ggattacagg tgtgcactac catgcccagc taatttttgt
     6061 gtttttagta gagactgggt ttcaccatgt tggccaggct cgtctcaaac tcctgacctc
     6121 aggtgatcta cccgcctcca cctcccaaag tgttgtgatt acaggcgtga gccaccacac
     6181 ccagcccttt cactaatttt tggcagagtt cacaatccca tcctatgtca gaaacttgaa
     6241 cctaacttaa gaaattttga gtcatttttt tggaggactg aggctttgtt ctacctactc
     6301 ttgaagaatc ctgatgaaat attcaaacac ataaagggat ggccctggag gtctatcctg
     6361 catacctgga gagcttctct ggtcctgccc accttgaaag ttgcagactt tgttgtaaca
     6421 tctatgtgat gagcttccct gtgaagcccc atttgacagg tgggccagca gtgttttatc
     6481 agcactctga tggcccacat ttcccaggaa aacttcttga aacttaccgt tgtgggcagt
     6541 ggtcagagcg tgtagttctt atgactgtct tgtttcatct gtggagcacg tgtggagcac
     6601 gttggtacag cagtttctgt ggggtttggc atgggtgatt ttggttagta accatcctcc
     6661 aattgttgct tgtgaaagtc attcataact tgttcctttt acttagtttt gttgttactg
     6721 ctgttgttgt tggtttggtt ggttttgaga ccgggtctgg ctctgtagcc caagctggaa
     6781 tgcggtggcg cgatcatggc tccctgcagc ctcaaactcc tgggctcaag caatcctcct
     6841 gcctcagcct cccaagcagc tgggaagata ggcacgtgcc accacacccg gctaattttt
     6901 tctttatttt ttttgtagag atgggggtct cactatgttg cccaggctgg tctcaaactc
     6961 ctgggctcaa gcgatccttt ggcctcggcc tcccaaagtg catgagccac catgcctggc
     7021 ctgtttagtt ttgtttcaag ttgaaatacc tttcttgtgt tttctaatta gaaaagtaat
     7081 atctactcat tgtaaaaact caaacagtgc agaaatgtag aaagtagaaa gtgtaagtcc
     7141 ctggttgtcc cttctgcctg agacaaccac tgctcacagt ttgatgtata tccttccaga
     7201 gactctcaaa tttaagcaaa taatttttat taccatgtct ttttatttga agacgtacat
     7261 ttgcctccaa agttcaacac aagttcaact gaccatatcc ttccatgacc tgaatagatg
     7321 ctatccttta tcacgatgtt caattgcctt tgaaagagag tagtccaggt atattcctga
     7381 tcaaaatttg gcatttttga tgatactact ctacacagat cagactcatg tgcagaatcg
     7441 tgcctggaga gagaggtttg gttaagacag agatttctgg aaacattcaa attgcaaatg
     7501 gaaacttgaa acccacaatc taatgaggaa tgtactggaa aaataatctg aagagttgac
     7561 aaattgtgta ctagattgaa cacatggaat gcaatgcaat gagactttct gcactaaaac
     7621 ttatcctcat atgtacaaca atgatgtgtg tattatataa cagtgatgtg tacatttctg
     7681 acaccccata cataatatac acagtttgta taaatgcata catttaaaaa tatatatgta
     7741 caatacagct aacataaaac tgtagtacgc ctgaaggata ttactagtgc ctaatattga
     7801 gtatgagtca ctgcgtgttc gcatcaactt ggaagtgcag taattgttat aaaattaatc
     7861 agtgcagcca acattattta tgaatcacat ctttgaaact gtgcagtagc atatacatat
     7921 atatttttaa ataacatttt tcacagtttt ccagagttac tgttgaaatc tgcatcacca
     7981 aaaaaaaaaa aaagcaagat ttttttaaca atgtagacac tcttcagacc cagtaatctg
     8041 cgtgtgattt cctatttgta gattcccaag agactttagc agtcaccagc cttaatgcat
     8101 gtacaggata ttattgtgac ttaatttatc tgcagttttt aatccatgtg aaattggaat
     8161 ttataaacgg acttggatta aacatgtctg cctttctaag gttgcaaatg ttacattaaa
     8221 tgatttatgt tgtaaatgag agaagttgag ttgtacgtaa aagacatcca ccaatttctg
     8281 tgaatatttt tacacggaaa cttgagaatc aaaatacgtt caagtcagtt gttctggctc
     8341 ccagtttata atctcgaaaa gcaatctttt tgtttcataa atggcttaaa tgtagctccc
     8401 acagattatc agtatgaagt ctgacatttt tcatggaaaa gaagtatgtt tttcttcatt
     8461 ataactatag tttactattt gtctttgcta tttcctgata catacacata cgcacacacg
     8521 cgcgcacatg catgcacgta tgcacatgca cgctcccagt gtcccagtga aggctcaaca
     8581 ggaagcttga gaaggtcatc tcaatggcca cagggttcct gttcaaccag tagccctggg
     8641 atgccgcttt gcttgtcatt ctttagtgtc acaagttagt tccctgactt gtgtgcagtc
     8701 agggaactgc acacaactga aaccaacatg gtggaatcat gtttgcaata tttggttgag
     8761 taaaataaga taattttgaa ctaaagaaga cggaggcatt agaccagtgg cttgacatga
     8821 agcatcccct atgtgggagg gtaagtttgt taacttcatt tttagtctta tctccaaccc
     8881 ttttccttat cctcctcccc aaactaaaga agcagaaaca gcaacaaaag caaaaccatc
     8941 ccttcacctg atccagccca tgtccttaga ttcaaatatc ctttgtcttt ttcccattat
     9001 accatctgac ataactcact cgtgtctaga gagaaaaatg cagaaagggt ttgtttgcag
     9061 atgggcagtt cagttgagtc ctcattaacc aggcagaggg gtgcagaaga agcctgactc
     9121 agggtcacca tggagaactc ctcatcccag agatgggtgg gacaggaact tggctttgct
     9181 tcaccaacca ctgagcttga tactctttca gaggtggttt gtgctaagaa acacccagca
     9241 gtctttcaag aacgttctct tctgtagctt tgcctgctac acagctgaag tctgtcccta
     9301 taagaattat atttgtaaaa agtaatacat tttcgaagaa acccggatca agtggaatct
     9361 tgaaagtttt tttaatgaaa ggggaaaaac aacctgacca cataaactgc acttccaacc
     9421 atttggaacc agagtttatg tcctcatttt cccattctca gaagagcaca gtttttcaat
     9481 attacaactt cctggcaaat agcacaagaa atgttcagac ttgagatttg tgttcccttt
     9541 gtccagatta ttctgtatat gtcaccaaag gggttaactt gtttgcttgg catgaggaaa
     9601 attataaact ccgttctcaa actaattaca tctgcccacg gttttggttt ttattatttt
     9661 aattggcact ccgaggcagc agggggtaaa ttttgtgttg gctgcaaggg gatttaggtt
     9721 cacagctctt actcacagta atgggaatca agggattaaa tctctgtgtg ttactttgaa
     9781 aatccacctc ttacagccaa ttttatagac attgatctga gggttgaatt cagggcacta
     9841 attgccagtc agtacaaggg tcagcatttg tgttgaagca ccaggggtat tgatgtagcc
     9901 tgaaaagctg aatcagggac ttgggttttg ttttgttttg ttttttttac ccatttttaa
     9961 aataggaaag ctcatccaga aatacttgga tctagacatg atataacata gcaccaatgt
    10021 ttaggatttt tagagtgctg ttgggtaatt aaaataatat caatgttcta
//
