LOCUS       NM_002025              13748 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ALF transcription elongation factor 2 (AFF2),
            transcript variant 1, mRNA.
ACCESSION   NM_002025
VERSION     NM_002025.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Shao,Y., Liu,Z., Song,X., Sun,R., Zhou,Y., Zhang,D., Sun,H.,
            Huang,J., Wu,C., Gu,W., Zheng,X. and Jiang,J.
  TITLE     ALKBH5/YTHDF2-mediated m6A modification of circAFF2 enhances
            radiosensitivity of colorectal cancer by inhibiting Cullin
            neddylation
  JOURNAL   Clin Transl Med 13 (7), e1318 (2023)
   PUBMED   37381158
  REMARK    GeneRIF: ALKBH5/YTHDF2-mediated m6A modification of circAFF2
            enhances radiosensitivity of colorectal cancer by inhibiting Cullin
            neddylation.
REFERENCE   2  (bases 1 to 13748)
  AUTHORS   Mu,J., Xu,Y. and Zhu,H.
  TITLE     [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms are
            correlated with disease susceptibility and immune infiltration in
            ankylosing spondylitis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (5), 741-748 (2023)
   PUBMED   37313815
  REMARK    GeneRIF: [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms
            are correlated with disease susceptibility and immune infiltration
            in ankylosing spondylitis].
REFERENCE   3  (bases 1 to 13748)
  AUTHORS   Kuo,Y.J., Lewis,J.S. Jr., Truong,T., Yeh,Y.C., Chernock,R.D.,
            Zhai,C., Chen,Y.A., Hongo,T., Lee,C.K., Shi,Q., Velez Torres,J.M.,
            Geromes,A.B., Chu,Y.H., Hsieh,M.S., Yamamoto,H., Weinreb,I. and
            Hang,J.F.
  TITLE     Nuclear expression of AFF2 C-terminus is a sensitive and specific
            ancillary marker for DEK::AFF2 carcinoma of the sinonasal tract
  JOURNAL   Mod Pathol 35 (11), 1587-1595 (2022)
   PUBMED   35701667
  REMARK    GeneRIF: Nuclear expression of AFF2 C-terminus is a sensitive and
            specific ancillary marker for DEK::AFF2 carcinoma of the sinonasal
            tract.
REFERENCE   4  (bases 1 to 13748)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   5  (bases 1 to 13748)
  AUTHORS   Silva,C., Maia,N., Santos,F., Rodrigues,B., Marques,I., Santos,R.
            and Jorge,P.
  TITLE     Development and validation in 500 female samples of a TP-PCR assay
            to identify AFF2 GCC expansions
  JOURNAL   Sci Rep 11 (1), 14676 (2021)
   PUBMED   34282157
  REMARK    GeneRIF: Development and validation in 500 female samples of a
            TP-PCR assay to identify AFF2 GCC expansions.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13748)
  AUTHORS   Allingham-Hawkins,D.J. and Ray,P.N.
  TITLE     FRAXE expansion is not a common etiological factor among
            developmentally delayed males
  JOURNAL   Am J Hum Genet 57 (1), 72-76 (1995)
   PUBMED   7541938
REFERENCE   7  (bases 1 to 13748)
  AUTHORS   Mulley,J.C., Yu,S., Loesch,D.Z., Hay,D.A., Donnelly,A.,
            Gedeon,A.K., Carbonell,P., Lopez,I., Glover,G., Gabarron,I. et al.
  TITLE     FRAXE and mental retardation
  JOURNAL   J Med Genet 32 (3), 162-169 (1995)
   PUBMED   7783162
REFERENCE   8  (bases 1 to 13748)
  AUTHORS   Knight,S.J., Voelckel,M.A., Hirst,M.C., Flannery,A.V., Moncla,A.
            and Davies,K.E.
  TITLE     Triplet repeat expansion at the FRAXE locus and X-linked mild
            mental handicap
  JOURNAL   Am J Hum Genet 55 (1), 81-86 (1994)
   PUBMED   8023854
REFERENCE   9  (bases 1 to 13748)
  AUTHORS   Knight,S.J., Flannery,A.V., Hirst,M.C., Campbell,L.,
            Christodoulou,Z., Phelps,S.R., Pointon,J., Middleton-Price,H.R.,
            Barnicoat,A., Pembrey,M.E. et al.
  TITLE     Trinucleotide repeat amplification and hypermethylation of a CpG
            island in FRAXE mental retardation
  JOURNAL   Cell 74 (1), 127-134 (1993)
   PUBMED   8334699
REFERENCE   10 (bases 1 to 13748)
  AUTHORS   Sutherland,G.R. and Baker,E.
  TITLE     Characterisation of a new rare fragile site easily confused with
            the fragile X
  JOURNAL   Hum Mol Genet 1 (2), 111-113 (1992)
   PUBMED   1301146
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002368.1, BC143744.1,
            BC132683.1, L76569.1 and AC231841.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 20, 2019 this sequence version replaced NM_002025.3.
            
            Summary: This gene encodes a putative transcriptional activator
            that is a member of the AF4\FMR2 gene family. This gene is
            associated with the folate-sensitive fragile X E locus on
            chromosome X. A repeat polymorphism in the fragile X E locus
            results in silencing of this gene causing Fragile X E syndrome.
            Fragile X E syndrome is a form of nonsyndromic X-linked cognitive
            disability. In addition, this gene contains 6-25 GCC repeats that
            are expanded to >200 repeats in the disease state. Alternate
            splicing results in multiple transcript variants.[provided by
            RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA2142853 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370460.7/ ENSP00000359489.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-430               AC002368.1         70925-71354
            431-1567            BC143744.1         1-1137
            1568-1654           BC132683.1         1126-1212
            1655-1725           BC143744.1         1138-1208
            1726-1743           L76569.1           1538-1555
            1744-3608           BC143744.1         1209-3073
            3609-3609           L76569.1           3415-3415
            3610-4487           BC143744.1         3075-3952
            4488-13748          AC231841.2         2353-11613          c
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..13748
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="ALF transcription elongation factor 2"
                     /db_xref="GeneID:2334"
                     /db_xref="HGNC:HGNC:3776"
                     /db_xref="MIM:300806"
     exon            1..528
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     CDS             482..4417
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein FMR-2; AF4/FMR2 family member 2"
                     /codon_start=1
                     /product="AF4/FMR2 family member 2 isoform 1"
                     /protein_id="NP_002016.2"
                     /db_xref="CCDS:CCDS14684.1"
                     /db_xref="GeneID:2334"
                     /db_xref="HGNC:HGNC:3776"
                     /db_xref="MIM:300806"
                     /translation="MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLF
                     SSGFDLFGEPYKVAEYTNKGDALANRVQNTLGNYDEMKNLLTNHSNQNHLVGIPKNSV
                     PQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVILNSTLIHSNRKSKPEWS
                     RDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNPSAKE
                     DSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYC
                     KTSMGQQKPTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSK
                     TKLPKFTILQTSEVSLPSDPSCVEEILREMTHSWPTPLTSMHTAGHSEQSTFSIPGQE
                     SQHLTPGFTLQKWNDPTTRASTKSVSFKSMLEDDLKLSSDEDDLEPVKTLTTQCTATE
                     LYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDTESSTTDSE
                     SNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPM
                     EVQMKVKTNASQVPAEPKERPLLSLIREKARPRPTQKIPETKALKHKLSTTSETVSQR
                     TIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKESVELHDPPRGRNKATAHKPAPRK
                     EPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVLPPCIISG
                     GNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSPIPVMQTEILSPLRDHENL
                     KNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPKGKRKHK
                     PIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFP
                     PPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTGQITSTKPKRTEGKFCATFKGISVNE
                     GDTPKKASSATITVTNTAIATATVTATAIVTTTVTATATATATTTTTTTTISTITSTI
                     TTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHKADA
                     LFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSETVELLRYAMRLKNFASP
                     LASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIPSPWV
                     SNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDM
                     ADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYVRQGLCWLRIDAHLL"
     exon            529..661
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            662..1522
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1567
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1654
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1691
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1743
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1840
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1878
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2038
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..3049
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3171
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3394
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3684
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3685..3748
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..3885
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3886..3957
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3958..4051
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            4052..4104
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4295
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            4296..13748
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8788..8793
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="hexamer: AATAAA"
     polyA_site      8812
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="major polyA site"
     regulatory      13725..13730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="hexamer: AATAAA"
     polyA_site      13748
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
ORIGIN      
        1 gccgccgcct gtgcagccgc tgccgccgcc gccgccgccg ccgccgccgc cgccgccgcc
       61 gccgccgctg ccgccccggc tgccgcgccg cgccgctgcc tctgccccgg ccgcccccgc
      121 cgccgctgcc gccgccggcc cgcagccagc caggcgggcg gcccagcccg cctgagcccg
      181 cagcggctgc cgccgcagcg tcgggtcgct gggtgcgcgg gctaccgcgg accgagcgga
      241 cccgagtggg cgaccaggcg cttgcccgcc cagtgccact gccgccgctt cctcgccgga
      301 gcacaggacc agacacctcc agcgcccgct gctgctgccg atgcggcccg gacactttta
      361 gctgggcggg agggctggag agccgggggc cgccgagaac cgccagcgag ctgtgccgag
      421 agccgcgccg acccgctgcg atcagggaca ggcgcccgcc cgccgccgcc gcctggccgc
      481 tatggatcta ttcgactttt tcagagactg ggacttggag cagcagtgtc actatgaaca
      541 agaccgtagt gcacttaaaa aaagggaatg ggagcggagg aatcaagaag tccagcaaga
      601 agacgatctc ttttcttcag gctttgatct ttttggggag ccatacaagg tagctgaata
      661 tacaaacaaa ggtgatgcac ttgccaaccg agtccagaac acgcttggaa actatgatga
      721 aatgaagaat ttgctaacta accattctaa tcagaatcac ctagtgggaa ttccaaagaa
      781 ttctgtgccc cagaatccca acaacaaaaa tgaaccaagc ttttttccag aacaaaagaa
      841 cagaataatt ccacctcacc aggataatac ccatccttca gcaccaatgc ctccaccttc
      901 tgttgtgata ctgaattcaa ctctaataca cagcaacaga aaatcaaaac ctgagtggtc
      961 acgtgatagt cataacccta gcactgtact ggcaagccag gccagtggtc agccaaacaa
     1021 gatgcagact ttgacacagg accagtctca agccaaactg gaagacttct ttgtctaccc
     1081 agctgaacag ccccagattg gagaagttga agagtcaaac ccatctgcaa aggaagacag
     1141 taaccctaat tctagtggag aagatgcttt caaagaaatc tttcaatcca attcaccgga
     1201 agaatctgaa ttcgccgtgc aagcgcctgg gtctccccta gtggcttcct ctttattagc
     1261 tcctagcagt ggcctttcag ttcaaaactt cccaccaggg ctttactgca aaacaagcat
     1321 ggggcagcaa aagccaactg catacgtcag acccatggat ggccaggacc aggcaccgga
     1381 catctcacca acactgaaac cttcaattga atttgagaac agctttggga atctgtcatt
     1441 tggaacactc ttggatggaa aacccagtgc agccagttca aagactaaac tgccaaagtt
     1501 caccatcctc caaacaagtg aagtaagcct tcccagtgat ccaagctgtg ttgaagaaat
     1561 cttgcgggag atgacccatt cctggcctac tcctctcact tccatgcata ctgctggaca
     1621 ctctgagcag agcacctttt ccatcccagg acaggaatcg cagcatctga ccccaggatt
     1681 caccttacaa aagtggaatg acccaaccac cagagcttct acaaagtcag tgtctttcaa
     1741 atcgatgctt gaggatgacc tgaagctgag cagtgatgaa gatgaccttg agcctgtgaa
     1801 gaccttgacc actcagtgca ctgccactga gctctaccag gctgttgaaa aggcaaaacc
     1861 taggaataat cctgtgaacc cacccttggc cactccccag cccccacctg cagtgcaagc
     1921 cagcgggggt tctggcagct ccagcgaatc ggagagcagc tctgagtcgg attcagacac
     1981 tgaaagtagc accactgaca gcgaatctaa tgaggcacct cgtgtggcaa ctccagagcc
     2041 tgagccaccc tcaaccaaca agtggcaact ggataaatgg cttaacaaag tgacatccca
     2101 gaacaagtct tttatttgtg gccaaaatga aacacccatg gagactattt ctctgcctcc
     2161 tccaatcatc caaccaatgg aagtccagat gaaagtgaag acgaatgcca gtcaggtccc
     2221 agctgaaccc aaagaaaggc ctctcctcag tctcattagg gagaaagccc gtccacggcc
     2281 cactcagaaa attccagaaa caaaggcttt gaagcataag ttgtcaacaa ctagtgagac
     2341 agtgtctcaa aggacaattg ggaaaaaaca gcccaaaaaa gttgagaaga acaccagcac
     2401 tgacgagttt acctggccca aaccaaatat taccagcagc actcccaaag aaaaagaaag
     2461 tgtggagctt catgacccac caagaggccg caacaaagcc actgcccaca aaccagcccc
     2521 taggaaagaa ccaagaccta acatcccttt ggctcccgag aagaagaagt acagagggcc
     2581 tggcaagatt gtgccaaagt ctcgggaatt cattgaaaca gattcatcta catctgactc
     2641 caacacagat caggaagaga ccctgcaaat caaagtcctg cctccgtgca ttatttctgg
     2701 aggtaatact gccaaatcca aggaaatctg tggtgccagc ctgaccctca gcaccttaat
     2761 gagtagcagt ggcagcaaca acaacttatc catcagtaat gaagagccaa cattttcacc
     2821 tattcctgtc atgcaaactg aaatcctgtc ccctctgcga gatcatgaga acctgaaaaa
     2881 cctctgggtg aagattgacc ttgacttact ctctagagta cctggccaca gctcactcca
     2941 tgcagcacct gccaagccag accacaagga gactgccaca aaacccaagc gtcagacagc
     3001 tgtcacagct gtggagaaac cagcccctaa gggcaaacgt aagcacaagc caatagaagt
     3061 tgcagagaag atccctgaga agaagcagcg cctggaggag gccacaacta tctgcttgct
     3121 ccctccttgc atctcaccag ccccacccca caagcctccc aacactagag aaaataattc
     3181 atccaggaga gcaaatagaa gaaaggaaga aaaactattt cctcctccac tttccccact
     3241 gccagaggac cctccacgcc gcagaaatgt cagtggcaat aatggtccct ttggtcaaga
     3301 caaaaacatc gccatgactg gacaaatcac atctaccaaa cctaagagaa ctgaaggcaa
     3361 attctgtgct actttcaaag ggatatcggt aaatgaggga gacactccaa aaaaggcatc
     3421 ctctgccacc atcactgtca ccaatactgc tattgccact gctactgtca ctgctactgc
     3481 cattgtcacc accactgtca cagctactgc caccgccacg gccaccacca caactactac
     3541 cactaccatt tccaccatca cctctaccat cactactggc ctcatggata gcagtcacct
     3601 ggagatgacg tcctgggcgg ctctgcccct tctatccagc agcagcacta atgtccggag
     3661 acccaagctc acttttgatg actcggttca caatgctgat tattacatgc aagaagctaa
     3721 gaagctgaag cacaaagctg atgcactgtt cgagaaattt ggcaaagctg tgaattatgc
     3781 tgatgccgcc ctctccttca ctgaatgtgg caatgccatg gaacgcgacc ctctggaagc
     3841 aaagtcccca tacaccatgt actctgagac tgtggagctc ctcaggtatg caatgaggct
     3901 gaagaacttt gcaagtccct tggcttcgga tggggacaaa aagctagcag tactatgcta
     3961 ccgatgttta tcactcctct atttgagaat gtttaagctg aagaaggacc atgctatgaa
     4021 gtactccaga tcactgatgg aatattttaa gcaaaatgct tcaaaagtcg cacagatacc
     4081 ctctccatgg gtaagcaatg gaaagaacac tccatcccca gtgtctctca acaacgtctc
     4141 ccccatcaac gcaatgggga actgtaacaa tggcccagtc accattcccc agcgcattca
     4201 ccacatggct gccagccacg tcaacatcac tagcaatgtg ttacggggct atgaacactg
     4261 ggatatggcc gacaaactga caagagaaaa caaagaattc tttggtgatc tggacacgct
     4321 gatggggcct ctgacccagc acagcagcat gaccaatctt gtccgctacg ttcgccaagg
     4381 actgtgttgg ctgcgcatcg atgcccactt gttgtagtgg gtgttctcag atctctagca
     4441 tcacgaccca tcactctacc tctaccagcg cactgatggt cactggtgga actccactca
     4501 ctggggaacg ttctctttgg ttatgtttgt ttttatgctt cttttgttat ctgtaaaaaa
     4561 cagaagtcat tgtaagttga cactacaact taagggcagt gtacgtttta ttacttagtc
     4621 attttttttc ttttagcatt tgatatgcat ttctcagatt ccaccatctt tttgtgcttt
     4681 atggaatgac agtccctaca atattgtttt aagcccacac tacccaaaac aaagaatggg
     4741 aagcacttgt gataaagaca ggctcctgag aaatgcaaca agtggtctta catatacatg
     4801 agaacttaga cacaagggac catcccccaa actctactct tatacccaga aaagaacata
     4861 tttcagaatc tgtcaaactt ttgtgtatcc cacagattca atcttcaggt gagaattttc
     4921 attgtcaaaa cccactggtt agatgttgta gcaacatcat aaaatcaaga gtatcaagaa
     4981 aataaatgag catagcaatg ctactcttaa aaagatgcta tgccacacaa ccagaggact
     5041 ttcttgttag catccctttc ctgattccct attttgttaa ttttaatgat aagaagaaag
     5101 ggtgacattt attttgacaa gttttaggca tcagctggca tcagtgtttt tcaactccat
     5161 tatttgaagt gtaaatcctc acctggggtt ctctgtgtgc aaagctgtcc ttttgaagaa
     5221 cagtttggtt gatgcatgcc ttagtagcca aaatgctaca ctctagactt acaagtggga
     5281 gttaagagag gtctggaaag tgtccaacaa ggaattcaca cctctgcctc ctttgcaaca
     5341 acaacattta cacagttggt aagtgggtcc ataactggca ggatttttaa attgtatttt
     5401 gctcaaatct atgggaacaa aagtcaaggt atcactacct agaagtaatg atatacagtt
     5461 ttcttcctag tggcttgaaa atctggactt cctcaattat tattcacatt ttctctctta
     5521 taggttttct gttttctact ttcttttttc tcttatctgt gtttcccttt cctttgtttg
     5581 gctcattaac ttttgactga attacaatta ctccttttat taaagtccat attattgtga
     5641 atcatttcca tgaaaatttc taagaaaact ccaaactctc taaatagtag ctaactttta
     5701 tttttttaaa atgagtcgtg gggtagtgct tcaccttgag atgctttgaa agagccctaa
     5761 acattgggaa ccattcacct aatttggaga catttctcac tggttgtgac taccccctta
     5821 tgatccttca cattcatttt atgtccctaa acatcacaat gtaaatatca tttttgatgt
     5881 tccagctcac cagaagattc ttacacttgg ggtaaacact atccatgcat tacttactgg
     5941 taattacctg ctggtatata attccatgta gcctttaata tgctgggtta tcaaattctg
     6001 ttcactgagt tatgaccaga taaataatag atatgcacat gaaagatgca aacttgtgtg
     6061 attattaaag ccagccatgc aggtccatga tagaaacagc aggtgatgac tctgcactct
     6121 cattgtcaag gttagatata tccccagttg caaaacagcc agacttgagc tgtgctctgg
     6181 tcatctttga gtttaaggcc ttttgttgta taaggctgtg gaagttgtac tccaatggct
     6241 gaagccatgt tgttaatatg gctgatggga gcatccctgc agctgaaccc agcacttttt
     6301 atgctcccac tgtggttgag ctttatgttt acagtctcag caacaacact tatgcatcca
     6361 aacactcaca aatgaaacct gaaagaatct tttctgagcc tcttaaaaga ggaaaatgat
     6421 gataacatta aagactctga acacccaagg ttggtgtcac atataaaaat taagctgatg
     6481 actttgcagt gactcaagtt gtctctttat catggtttac caggtagagt gcctggctat
     6541 tactatataa tgaagcccac tggcttgact tgtaagttca acctaaacca caatcctaga
     6601 ccatcatgga tttaggagta gattcttctt gaaatcccac atccagaaac tagacattag
     6661 aatgttgagg cagtttccca gagaaacaag catattgcct catggatgaa agacttgtag
     6721 ttctagtttc agtgacttgt tatatctact tacatacaac agggaggcaa gaggattctc
     6781 tgtcatctct ggtgactgag tgtaaaatat gtgccaagtc tgcagcacag tgaccaaatc
     6841 tgacaatcga gctctggatc accacttgat tatgtagtag actcatttat aaagcagctt
     6901 aggaactaat taaacatgga ggatgaatta ccttcctatc ccttgagata agacatcttt
     6961 cagtttcatg attaaggatt gttgctgttt tatagttact ctgttcatca cagtgtaaat
     7021 ggtgatgcgt gtcgtaggtg tgcagctatt tgagggacta agggatggag atattctgtc
     7081 aaatgaatct cttcagtata ccagtttgtg ggagggatat gagacatgtg gatggcagtg
     7141 agagatcgtg cctctagatc ttgatggagg cttggtgaga cacacttaaa taagcacgtg
     7201 gaggttagaa tagagggcag agtaaaagga agctccatct gagcaagtac accaaatgat
     7261 ctcagccctg caacttgacc caggtagggc caccactacg ccttcacttg tcacccaagc
     7321 tccaaccaca gagagtttga caagtttgtg ttatgatgtt ggcttggctt tgtattttta
     7381 attaactttg gatttttagt ggttttgtca tataactgtc tgagtttggt aggtaggatt
     7441 actttgaaaa gggtttacta gtgtggtcct ccgggtagaa tttagctgta agcatgttgt
     7501 tagccagcct gtagactgtt aattacttaa taatctcatt gggaaaatac tagtagtttt
     7561 atatttggat gacataattg gaaaagcaga ttagctgcta ctacttttaa aagacttaag
     7621 gtcgggatgc ctttttttcc atgtaaggaa atgaaaagac caaaatcttc aggcaaaaag
     7681 caagttgcaa aattagaaac cattggctaa aaatgtgttt tgttgagttt ccaaatggat
     7741 gaattttcat ttggacatta catcactaaa ttcattagat tttgtctgca ttggaaagat
     7801 actcttctag catatctttc ccaaagatat ctaatttgga ttctgtttca tgcaaatttg
     7861 catcccggag gttgaagttg gagtttgagg ttggaaaata tctttgaagg cagaatcagt
     7921 tgagttgtga gggtgaagcc tcacatactt ctcaacagac atgataaaat tcacctgcat
     7981 gagttggcag gtgggagaac caaactggat cactgggtaa gactactcag taaagcaatg
     8041 aactgcttgc ttagagaagc atcactatcc ccattgagaa aaatgtgtgg caagatgata
     8101 cagctacaca gtatcaaatg aatgggtcaa ttcagcaccc ccaaatttaa ttctgtgggg
     8161 aaaaattatt gagccagttg tcagtgttct gttacatgac tggcagacta aattcttcat
     8221 cgttgttgtt attgttgttg ttgtttctca ttttcactcg cacggcctta ttctcataat
     8281 taaaatctaa ttcattttct ctttagtgtt agtagactcc aacaacagaa gtggcatctg
     8341 tgtattcata atcagcattt accctggcag gagactaatc agataggccg gtctcagaca
     8401 ttaatcctac catctgatat ttttggtgaa ggaaaaagta ttaattctct ttccatcctc
     8461 ctcctcagaa atatagaagc cctctttacc aaaatcatca cattttactc tgtaatctac
     8521 cagctaaaag aaaattgcat tgtggggagg gcagaaaggg ggtgggggtg ggggcggggg
     8581 ggtggggggt ggggaagccc cacaaagcca gattgcagtt cttgcccctt tttgcgtctg
     8641 acatgagatg ttaaagaatt attcatagtg ctcacattgg gttaggggac actgaactgc
     8701 tttttagatc catgatcagt catcattctt ctaagagatt ggagctttgc tgtttcatta
     8761 actgtgcagt gtagactaat ggtgtttaat aaaaatcatt caaaatttca aactcttttg
     8821 ccagtgacct caattttgtt ggctctgtga tttgtatcag actttgagga gggaaggggg
     8881 aagtgaagga agcctacgtc caggcccctg acaggatgct gcagtagcaa gctcaagctc
     8941 gcctgcctgc cagcagttgc tggtgagcag cagcatgcag accagctgtg ggaagcctcc
     9001 tgaagaatgc cccagctgat gctttcagct gggaatagtt tgttcctatt ggggaactca
     9061 ttgttctcca gtctctgcag caggaagcca gctgtcatat tcggagggaa tttcagatgc
     9121 tttacctttt tggttttgtc ctgcatcact catgtggcta cgaaagtgtc tctgagaata
     9181 gagcccaatg tggtgacaat gggtagtcaa atgcacccca gatgctcaag ccctgttgtg
     9241 gttctgcagt gtttatgaaa ttgggaggaa ggagaccctg gacagtaagc aaaattggag
     9301 acactccaac gaggctaagt taatgccgtg ttgcccagaa caagatctag cttctcattt
     9361 ggtcagccta gcatgcaacc agtggtgtgc tggtaaaatg tttaacaacc agctcgctga
     9421 gaatagaaag cacctggttt gcaccatttg ccaatttcca tggcataaat actaccactt
     9481 tagatgattt taagctacca actgtgatgt cactgaacac atggttggaa agagatgcac
     9541 gcagttggct cttgcaagcc tgggcaaaaa tgcttcaaca cgccactgga tgcagccagt
     9601 cagagggttc atatttaata tatgtgttca tgtggacaca cacagacaca cacacacaaa
     9661 ctcaccctta cacacacact tcgatgacta aaacaattac atagttttaa gatatgaatc
     9721 aatgtgtgaa tgtagaaagc ttatgataag gccctagagg tatgggttgc cctggaagcc
     9781 taggttttaa gcaggagaat agctgagaag aatgaagccc tcctgagctg aaaggagaga
     9841 tggatcaatg gagatggttc catcatctcc ttccatatct cacaggtaaa atgggcactc
     9901 agaaaaccct cacgattgat tttttaaaaa gataagtgag tgttttttat tttattatta
     9961 ttgtcatcat tattttgatt tacaaatgct atttgtaact tttacatgta actaggataa
    10021 agtatttacg ggaactctat ggagaatagc acaatccaga atttactgtg tttttctttt
    10081 atgtgacgtg gaaactcagt aattctccca ccttcacatt gttgttcata agaattttac
    10141 tttagttatt agggaatcta agttttttgt taacatttgt ttttagttaa aagtatctac
    10201 ttactgtttt agctctgaac tcaaaccaga atatctctgt atcaattgca tgactattca
    10261 gaaacaataa tccaaaccaa aataattctt tttccaccca gtacgaagaa aactaagctc
    10321 agtaacaaga aggcataaac taaagtatat aatgaggctt tcattaaata cacacacaca
    10381 cacactcaca cacacacaca tacacttttt aaatttttaa attaggcctc cacacataaa
    10441 tcattttgaa agtagaatag aaaatctcaa agaattcatt ctcctggtcc tgtgcatctt
    10501 ctgcagttaa taagaggttt gtatctggaa agatggaaga acttgttcta aaatcttatt
    10561 tttcaaaaaa aaatttccat tttctctctg ggcctgtatc catggttgaa tgttagccct
    10621 ggaggagatc catgtcttac tcgctctttc tggcccttct gtcttttgcc tctgcaattc
    10681 tttttgtagc tggcacgata gcagggactg ggggtctatc ctttcatggt attgctacaa
    10741 tatttgtcct tactggaaaa tggtaacatc cgggtctgat ttaattggca ttacacttac
    10801 acagggactc tgagcacccc cgtcaccaca ccagacagtg gaccagtttt cacagctaca
    10861 aagagctaga aatgtgttta acatcatcca gtgcatcccc taattcaaaa ccatcctcac
    10921 taatcaatca tattcaccca taaatattac aaatgagatt gattccatct caagacaatt
    10981 tgtcaaatac ttaattttct tcctggatga ttctacttac tggatatttt agaaagagaa
    11041 atgtctgaga taaaatccct cacatttact caatataaca aattactgtt tctactccta
    11101 ttctgagtag tgcttctgaa gattgtttgc tgtagtgttg tctttgataa aatgaatgtc
    11161 agtagtgagc cttttagaga taccatgctc agaaatcctc tttgggatca gaagatacct
    11221 aaaattctcc ccttttgccc acttggttag atgagtgata tattctttgg atcctgcaaa
    11281 gaagagattg gtttattttc ttttctggtg gtggtagtgg ttgtatctgt ggctgtgatg
    11341 gttgttgtta cttgtctctc tctctctctg gctctggctt ttgctttcct gctagtgttc
    11401 tttctctttc caaacaaata gttaaattaa atgtgagctt ctgaattgta cttgttcata
    11461 ctttcaaaac ataacagatt aataaaaata gatgtgtcct gatttaaaac atgccccctg
    11521 gaaaggcatg ctgtattatg aaatcgtgat aatataactg cattattaca tggcagtata
    11581 aatattagtc tgttgaattc atttgtccaa ttgtataact ttgtggagca gtgttttgac
    11641 ctttgataca taattctgga gcaagtggag tggttgcagg cagatgagac agtgttatat
    11701 caggattttt caatcaactt tagttggagg cctggcaatt acaaacatct tcagatgttt
    11761 ctgtaaccat tataaatatg aaaaaaacct cttcaaaaaa tttcccatag tacttcagtc
    11821 aagacttttt aggtttatct tttttttttc atttctcctt ttccttttcc attatttttc
    11881 gatggggggg ttgttatcat tgactgaaga aatattttga ttgcaatggt ctctctctct
    11941 ctccccctct ctctctctct cctctattct ttcctccttc cctctgtcca tcacccctca
    12001 ttaaaatatt gaaatctgga gtctttgata aatctgcatt agaccaggct atatgctagg
    12061 aatgaaatct gggcaaatat cgatgggttt tcaaagaatg ctccatgttc attgggccct
    12121 ttcacacccc acagtgataa atgaaaagga tagaggtagt tttttcaaaa gagcacttta
    12181 ataatatcct ctgagaccta atgcagttta acaaatgact ccacctattt ttccagtagg
    12241 taaattgact gagacttgca aaatacccct gagagttgtc aggggtgtct tctgcctggt
    12301 ctatagcgtg tgtgtttgct ttgtatctaa caggcacatt cacgtctcgt gtactcatat
    12361 gaagtatttc ctaacattcc cattagcctg tatataagaa tcagaaagat aatcccaaca
    12421 tgttgtaaat gaagatgtga ctctataacc tttctcttct tcctggaaaa aaaaggacat
    12481 tttcatgcat attttaaaca gaaattttgt atatttaagt gtcatagaaa atatttattg
    12541 agtaactggg acacaaatgg gaatttaatt gtcatcatat gctttgtgtg tggggatgct
    12601 taccaacacc atgtcgctgg accattgtgg caagccataa ctgcacaaag agtacacatc
    12661 gtcagtgtgt gtgtgtgtgt gtgtgtgcgc gcacacacgt gcgtgtgtgt gtccctgcat
    12721 gtgcaacatg tctagcttgc tgtccttcat gggattttag ctttcccttc ttgaaaaaca
    12781 ttattttaca gttccaggag gccctggtta cattactata tgaaggcagt gatttgaaat
    12841 gaaaattcct ttcctcttgg aagctttggt cataatatca tggttcaatt aaacggattc
    12901 caccggactt tgtgatgaaa aaggctctgt taaaatccaa ttgagtttcc aagaggaaat
    12961 tgtagtaggt caagatgcat gagagggaag atggaggcca cctcaactgg agaacatgag
    13021 ctgagttgag ccctcagtgt tgaagttgac ttgctccaag ctgcagtcta aaaccctggg
    13081 gcccgtgcct ggcctatgct ccctcccaag taagtagagg agcagaacca tcaggaacag
    13141 cctgcctggc tcctatgaag aaaacttcct gacgtcctgt ccccaaagga agaccctttc
    13201 cccaagggca ccccaggtgg ccattaaatt gtgatgatca ttcagaaagt gcccccttgg
    13261 ctttatgaga atccaattag tcttctgaac caccttttct tgggtgcaga tttccaacat
    13321 tcatgctcat tgcagatcca ccaactgtca ctgttcttaa caagcatgct cgtcttgtca
    13381 gaatttcagt aagttccaat ttcctgtaca gaccagggta aactgttcta aaatcaatca
    13441 attaatgaaa tgttatctgg tttttaaaag ctggtttcat gtgctttatg tgtataaaac
    13501 tatatctgcc tgtgtggctt tgcatttcaa atgtgtggcg cacaagcgtt ttgttggtgc
    13561 tttgttctca gtacagtaac tctgtgtaca aacattttaa tgtggttttg ttgttttcca
    13621 acaagatgtc tctgtaaaaa tgatattggc tgagctggtg cgttggtttc tctcatagag
    13681 gcattaacta tactgccaat gcattgaatt atttaaaaat gcaaaataaa atttttatga
    13741 aaatctca
//
