LOCUS       NM_001169122           13643 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens ALF transcription elongation factor 2 (AFF2),
            transcript variant 2, mRNA.
ACCESSION   NM_001169122
VERSION     NM_001169122.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Shao,Y., Liu,Z., Song,X., Sun,R., Zhou,Y., Zhang,D., Sun,H.,
            Huang,J., Wu,C., Gu,W., Zheng,X. and Jiang,J.
  TITLE     ALKBH5/YTHDF2-mediated m6A modification of circAFF2 enhances
            radiosensitivity of colorectal cancer by inhibiting Cullin
            neddylation
  JOURNAL   Clin Transl Med 13 (7), e1318 (2023)
   PUBMED   37381158
  REMARK    GeneRIF: ALKBH5/YTHDF2-mediated m6A modification of circAFF2
            enhances radiosensitivity of colorectal cancer by inhibiting Cullin
            neddylation.
REFERENCE   2  (bases 1 to 13643)
  AUTHORS   Mu,J., Xu,Y. and Zhu,H.
  TITLE     [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms are
            correlated with disease susceptibility and immune infiltration in
            ankylosing spondylitis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (5), 741-748 (2023)
   PUBMED   37313815
  REMARK    GeneRIF: [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms
            are correlated with disease susceptibility and immune infiltration
            in ankylosing spondylitis].
REFERENCE   3  (bases 1 to 13643)
  AUTHORS   Kuo,Y.J., Lewis,J.S. Jr., Truong,T., Yeh,Y.C., Chernock,R.D.,
            Zhai,C., Chen,Y.A., Hongo,T., Lee,C.K., Shi,Q., Velez Torres,J.M.,
            Geromes,A.B., Chu,Y.H., Hsieh,M.S., Yamamoto,H., Weinreb,I. and
            Hang,J.F.
  TITLE     Nuclear expression of AFF2 C-terminus is a sensitive and specific
            ancillary marker for DEK::AFF2 carcinoma of the sinonasal tract
  JOURNAL   Mod Pathol 35 (11), 1587-1595 (2022)
   PUBMED   35701667
  REMARK    GeneRIF: Nuclear expression of AFF2 C-terminus is a sensitive and
            specific ancillary marker for DEK::AFF2 carcinoma of the sinonasal
            tract.
REFERENCE   4  (bases 1 to 13643)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   5  (bases 1 to 13643)
  AUTHORS   Silva,C., Maia,N., Santos,F., Rodrigues,B., Marques,I., Santos,R.
            and Jorge,P.
  TITLE     Development and validation in 500 female samples of a TP-PCR assay
            to identify AFF2 GCC expansions
  JOURNAL   Sci Rep 11 (1), 14676 (2021)
   PUBMED   34282157
  REMARK    GeneRIF: Development and validation in 500 female samples of a
            TP-PCR assay to identify AFF2 GCC expansions.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 13643)
  AUTHORS   Allingham-Hawkins,D.J. and Ray,P.N.
  TITLE     FRAXE expansion is not a common etiological factor among
            developmentally delayed males
  JOURNAL   Am J Hum Genet 57 (1), 72-76 (1995)
   PUBMED   7541938
REFERENCE   7  (bases 1 to 13643)
  AUTHORS   Mulley,J.C., Yu,S., Loesch,D.Z., Hay,D.A., Donnelly,A.,
            Gedeon,A.K., Carbonell,P., Lopez,I., Glover,G., Gabarron,I. et al.
  TITLE     FRAXE and mental retardation
  JOURNAL   J Med Genet 32 (3), 162-169 (1995)
   PUBMED   7783162
REFERENCE   8  (bases 1 to 13643)
  AUTHORS   Knight,S.J., Voelckel,M.A., Hirst,M.C., Flannery,A.V., Moncla,A.
            and Davies,K.E.
  TITLE     Triplet repeat expansion at the FRAXE locus and X-linked mild
            mental handicap
  JOURNAL   Am J Hum Genet 55 (1), 81-86 (1994)
   PUBMED   8023854
REFERENCE   9  (bases 1 to 13643)
  AUTHORS   Knight,S.J., Flannery,A.V., Hirst,M.C., Campbell,L.,
            Christodoulou,Z., Phelps,S.R., Pointon,J., Middleton-Price,H.R.,
            Barnicoat,A., Pembrey,M.E. et al.
  TITLE     Trinucleotide repeat amplification and hypermethylation of a CpG
            island in FRAXE mental retardation
  JOURNAL   Cell 74 (1), 127-134 (1993)
   PUBMED   8334699
REFERENCE   10 (bases 1 to 13643)
  AUTHORS   Sutherland,G.R. and Baker,E.
  TITLE     Characterisation of a new rare fragile site easily confused with
            the fragile X
  JOURNAL   Hum Mol Genet 1 (2), 111-113 (1992)
   PUBMED   1301146
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002368.1, U48436.1, L76569.1
            and AC231841.2.
            
            On May 31, 2019 this sequence version replaced NM_001169122.1.
            
            Summary: This gene encodes a putative transcriptional activator
            that is a member of the AF4\FMR2 gene family. This gene is
            associated with the folate-sensitive fragile X E locus on
            chromosome X. A repeat polymorphism in the fragile X E locus
            results in silencing of this gene causing Fragile X E syndrome.
            Fragile X E syndrome is a form of nonsyndromic X-linked cognitive
            disability. In addition, this gene contains 6-25 GCC repeats that
            are expanded to >200 repeats in the disease state. Alternate
            splicing results in multiple transcript variants.[provided by
            RefSeq, Jul 2016].
            
            Transcript Variant: This variant (2) has multiple differences,
            compared to variant 1. The encoded isoform (2) is shorter than
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L76569.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2145743
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC002368.1         70925-70926
            3-107               U48436.1           1-105
            108-1790            L76569.1           19-1701
            1791-1791           AC231841.2         49425-49425         c
            1792-2024           L76569.1           1703-1935
            2025-2025           AC231841.2         47328-47328         c
            2026-6030           L76569.1           1937-5941
            6031-13643          AC231841.2         2353-9965           c
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq28"
     gene            1..13643
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="ALF transcription elongation factor 2"
                     /db_xref="GeneID:2334"
                     /db_xref="HGNC:HGNC:3776"
                     /db_xref="MIM:300806"
     exon            1..528
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     CDS             482..4312
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein FMR-2; AF4/FMR2 family member 2"
                     /codon_start=1
                     /product="AF4/FMR2 family member 2 isoform 2"
                     /protein_id="NP_001162593.1"
                     /db_xref="CCDS:CCDS78510.1"
                     /db_xref="GeneID:2334"
                     /db_xref="HGNC:HGNC:3776"
                     /db_xref="MIM:300806"
                     /translation="MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLF
                     SSGFDLFGEPYKTNKGDALANRVQNTLGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNP
                     NNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVILNSTLIHSNRKSKPEWSRDSH
                     NPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNPSAKEDSNP
                     NSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSM
                     GQQKPTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLP
                     KFTILQTSEVSLPSDPSCVEEILRESQHLTPGFTLQKWNDPTTRASTKSVSFKSMLED
                     DLKLSSDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGG
                     SGSSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQN
                     KSFICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIREKARPR
                     PTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKE
                     KESVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDS
                     STSDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISN
                     EEPTFSPIPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKET
                     ATKPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPP
                     HKPPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTG
                     QITSTKPKRTEGKFCATFKGISEGDTPKKASSATITVTNTAIATATVTATAIVTTTVT
                     ATATATATTTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTF
                     DDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSP
                     YTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKY
                     SRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRI
                     HHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYV
                     RQGLCWLRIDAHLL"
     exon            529..649
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            650..1510
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1555
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1592
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1644
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1741
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1779
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1939
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2950
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3072
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3073..3289
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3290..3579
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3580..3643
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3644..3780
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3852
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3853..3946
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..3999
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            4000..4190
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     exon            4191..13643
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8683..8688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="hexamer: AATAAA"
     polyA_site      8707
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="major polyA site"
     regulatory      13620..13625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
                     /note="hexamer: AATAAA"
     polyA_site      13643
                     /gene="AFF2"
                     /gene_synonym="FMR2; FMR2P; FRAXE; MRX2; OX19; XLID109"
ORIGIN      
        1 gccgccgcct gtgcagccgc tgccgccgcc gccgccgccg ccgccgccgc cgccgccgcc
       61 gccgccgctg ccgccccggc tgccgcgccg cgccgctgcc tctgccccgg ccgcccccgc
      121 cgccgctgcc gccgccggcc cgcagccagc caggcgggcg gcccagcccg cctgagcccg
      181 cagcggctgc cgccgcagcg tcgggtcgct gggtgcgcgg gctaccgcgg accgagcgga
      241 cccgagtggg cgaccaggcg cttgcccgcc cagtgccact gccgccgctt cctcgccgga
      301 gcacaggacc agacacctcc agcgcccgct gctgctgccg atgcggcccg gacactttta
      361 gctgggcggg agggctggag agccgggggc cgccgagaac cgccagcgag ctgtgccgag
      421 agccgcgccg acccgctgcg atcagggaca ggcgcccgcc cgccgccgcc gcctggccgc
      481 tatggatcta ttcgactttt tcagagactg ggacttggag cagcagtgtc actatgaaca
      541 agaccgtagt gcacttaaaa aaagggaatg ggagcggagg aatcaagaag tccagcaaga
      601 agacgatctc ttttcttcag gctttgatct ttttggggag ccatacaaga caaacaaagg
      661 tgatgcactt gccaaccgag tccagaacac gcttggaaac tatgatgaaa tgaagaattt
      721 gctaactaac cattctaatc agaatcacct agtgggaatt ccaaagaatt ctgtgcccca
      781 gaatcccaac aacaaaaatg aaccaagctt ttttccagaa caaaagaaca gaataattcc
      841 acctcaccag gataataccc atccttcagc accaatgcct ccaccttctg ttgtgatact
      901 gaattcaact ctaatacaca gcaacagaaa atcaaaacct gagtggtcac gtgatagtca
      961 taaccctagc actgtactgg caagccaggc cagtggtcag ccaaacaaga tgcagacttt
     1021 gacacaggac cagtctcaag ccaaactgga agacttcttt gtctacccag ctgaacagcc
     1081 ccagattgga gaagttgaag agtcaaaccc atctgcaaag gaagacagta accctaattc
     1141 tagtggagaa gatgctttca aagaaatctt tcaatccaat tcaccggaag aatctgaatt
     1201 cgccgtgcaa gcgcctgggt ctcccctagt ggcttcctct ttattagctc ctagcagtgg
     1261 cctttcagtt caaaacttcc caccagggct ttactgcaaa acaagcatgg ggcagcaaaa
     1321 gccaactgca tacgtcagac ccatggatgg ccaggaccag gcaccggaca tctcaccaac
     1381 actgaaacct tcaattgaat ttgagaacag ctttgggaat ctgtcatttg gaacactctt
     1441 ggatggaaaa cccagtgcag ccagttcaaa gactaaactg ccaaagttca ccatcctcca
     1501 aacaagtgaa gtaagccttc ccagtgatcc aagctgtgtt gaagaaatct tgcgggaatc
     1561 gcagcatctg accccaggat tcaccttaca aaagtggaat gacccaacca ccagagcttc
     1621 tacaaagtca gtgtctttca aatcgatgct tgaggatgac ctgaagctga gcagtgatga
     1681 agatgacctt gagcctgtga agaccttgac cactcagtgc actgccactg agctctacca
     1741 ggctgttgaa aaggcaaaac ctaggaataa tcctgtgaac ccacccttgg ccactcccca
     1801 gcccccacct gcagtgcaag ccagcggggg ttctggcagc tccagcgaat cggagagcag
     1861 ctctgagtcg gattcagaca ctgaaagtag caccactgac agcgaatcta atgaggcacc
     1921 tcgtgtggca actccagagc ctgagccacc ctcaaccaac aagtggcaac tggataaatg
     1981 gcttaacaaa gtgacatccc agaacaagtc ttttatttgt ggccaaaatg aaacacccat
     2041 ggagactatt tctctgcctc ctccaatcat ccaaccaatg gaagtccaga tgaaagtgaa
     2101 gacgaatgcc agtcaggtcc cagctgaacc caaagaaagg cctctcctca gtctcattag
     2161 ggagaaagcc cgtccacggc ccactcagaa aattccagaa acaaaggctt tgaagcataa
     2221 gttgtcaaca actagtgaga cagtgtctca aaggacaatt gggaaaaaac agcccaaaaa
     2281 agttgagaag aacaccagca ctgacgagtt tacctggccc aaaccaaata ttaccagcag
     2341 cactcccaaa gaaaaagaaa gtgtggagct tcatgaccca ccaagaggcc gcaacaaagc
     2401 cactgcccac aaaccagccc ctaggaaaga accaagacct aacatccctt tggctcccga
     2461 gaagaagaag tacagagggc ctggcaagat tgtgccaaag tctcgggaat tcattgaaac
     2521 agattcatct acatctgact ccaacacaga tcaggaagag accctgcaaa tcaaagtcct
     2581 gcctccgtgc attatttctg gaggtaatac tgccaaatcc aaggaaatct gtggtgccag
     2641 cctgaccctc agcaccttaa tgagtagcag tggcagcaac aacaacttat ccatcagtaa
     2701 tgaagagcca acattttcac ctattcctgt catgcaaact gaaatcctgt cccctctgcg
     2761 agatcatgag aacctgaaaa acctctgggt gaagattgac cttgacttac tctctagagt
     2821 acctggccac agctcactcc atgcagcacc tgccaagcca gaccacaagg agactgccac
     2881 aaaacccaag cgtcagacag ctgtcacagc tgtggagaaa ccagccccta agggcaaacg
     2941 taagcacaag ccaatagaag ttgcagagaa gatccctgag aagaagcagc gcctggagga
     3001 ggccacaact atctgcttgc tccctccttg catctcacca gccccacccc acaagcctcc
     3061 caacactaga gaaaataatt catccaggag agcaaataga agaaaggaag aaaaactatt
     3121 tcctcctcca ctttccccac tgccagagga ccctccacgc cgcagaaatg tcagtggcaa
     3181 taatggtccc tttggtcaag acaaaaacat cgccatgact ggacaaatca catctaccaa
     3241 acctaagaga actgaaggca aattctgtgc tactttcaaa gggatatcgg agggagacac
     3301 tccaaaaaag gcatcctctg ccaccatcac tgtcaccaat actgctattg ccactgctac
     3361 tgtcactgct actgccattg tcaccaccac tgtcacagct actgccaccg ccacggccac
     3421 caccacaact actaccacta ccatttccac catcacctct accatcacta ctggcctcat
     3481 ggatagcagt cacctggaga tgacgtcctg ggcggctctg ccccttctat ccagcagcag
     3541 cactaatgtc cggagaccca agctcacttt tgatgactcg gttcacaatg ctgattatta
     3601 catgcaagaa gctaagaagc tgaagcacaa agctgatgca ctgttcgaga aatttggcaa
     3661 agctgtgaat tatgctgatg ccgccctctc cttcactgaa tgtggcaatg ccatggaacg
     3721 cgaccctctg gaagcaaagt ccccatacac catgtactct gagactgtgg agctcctcag
     3781 gtatgcaatg aggctgaaga actttgcaag tcccttggct tcggatgggg acaaaaagct
     3841 agcagtacta tgctaccgat gtttatcact cctctatttg agaatgttta agctgaagaa
     3901 ggaccatgct atgaagtact ccagatcact gatggaatat tttaagcaaa atgcttcaaa
     3961 agtcgcacag ataccctctc catgggtaag caatggaaag aacactccat ccccagtgtc
     4021 tctcaacaac gtctccccca tcaacgcaat ggggaactgt aacaatggcc cagtcaccat
     4081 tccccagcgc attcaccaca tggctgccag ccacgtcaac atcactagca atgtgttacg
     4141 gggctatgaa cactgggata tggccgacaa actgacaaga gaaaacaaag aattctttgg
     4201 tgatctggac acgctgatgg ggcctctgac ccagcacagc agcatgacca atcttgtccg
     4261 ctacgttcgc caaggactgt gttggctgcg catcgatgcc cacttgttgt agtgggtgtt
     4321 ctcagatctc tagcatcacg acccatcact ctacctctac cagcgcactg atggtcactg
     4381 gtggaactcc actcactggg gaacgttctc tttggttatg tttgttttta tgcttctttt
     4441 gttatctgta aaaaacagaa gtcattgtaa gttgacacta caacttaagg gcagtgtacg
     4501 ttttattact tagtcatttt ttttctttta gcatttgata tgcatttctc agattccacc
     4561 atctttttgt gctttatgga atgacagtcc ctacaatatt gttttaagcc cacactaccc
     4621 aaaacaaaga atgggaagca cttgtgataa agacaggctc ctgagaaatg caacaagtgg
     4681 tcttacatat acatgagaac ttagacacaa gggaccatcc cccaaactct actcttatac
     4741 ccagaaaaga acatatttca gaatctgtca aacttttgtg tatcccacag attcaatctt
     4801 caggtgagaa ttttcattgt caaaacccac tggttagatg ttgtagcaac atcataaaat
     4861 caagagtatc aagaaaataa atgagcatag caatgctact cttaaaaaga tgctatgcca
     4921 cacaaccaga ggactttctt gttagcatcc ctttcctgat tccctatttt gttaatttta
     4981 atgataagaa gaaagggtga catttatttt gacaagtttt aggcatcagc tggcatcagt
     5041 gtttttcaac tccattattt gaagtgtaaa tcctcacctg gggttctctg tgtgcaaagc
     5101 tgtccttttg aagaacagtt tggttgatgc atgccttagt agccaaaatg ctacactcta
     5161 gacttacaag tgggagttaa gagaggtctg gaaagtgtcc aacaaggaat tcacacctct
     5221 gcctcctttg caacaacaac atttacacag ttggtaagtg ggtccataac tggcaggatt
     5281 tttaaattgt attttgctca aatctatggg aacaaaagtc aaggtatcac tacctagaag
     5341 taatgatata cagttttctt cctagtggct tgaaaatctg gacttcctca attattattc
     5401 acattttctc tcttataggt tttctgtttt ctactttctt ttttctctta tctgtgtttc
     5461 cctttccttt gtttggctca ttaacttttg actgaattac aattactcct tttattaaag
     5521 tccatattat tgtgaatcat ttccatgaaa atttctaaga aaactccaaa ctctctaaat
     5581 agtagctaac ttttattttt ttaaaatgag tcgtggggta gtgcttcacc ttgagatgct
     5641 ttgaaagagc cctaaacatt gggaaccatt cacctaattt ggagacattt ctcactggtt
     5701 gtgactaccc ccttatgatc cttcacattc attttatgtc cctaaacatc acaatgtaaa
     5761 tatcattttt gatgttccag ctcaccagaa gattcttaca cttggggtaa acactatcca
     5821 tgcattactt actggtaatt acctgctggt atataattcc atgtagcctt taatatgctg
     5881 ggttatcaaa ttctgttcac tgagttatga ccagataaat aatagatatg cacatgaaag
     5941 atgcaaactt gtgtgattat taaagccagc catgcaggtc catgatagaa acagcaggtg
     6001 atgactctgc actctcattg tcaaggttag atatatcccc agttgcaaaa cagccagact
     6061 tgagctgtgc tctggtcatc tttgagttta aggccttttg ttgtataagg ctgtggaagt
     6121 tgtactccaa tggctgaagc catgttgtta atatggctga tgggagcatc cctgcagctg
     6181 aacccagcac tttttatgct cccactgtgg ttgagcttta tgtttacagt ctcagcaaca
     6241 acacttatgc atccaaacac tcacaaatga aacctgaaag aatcttttct gagcctctta
     6301 aaagaggaaa atgatgataa cattaaagac tctgaacacc caaggttggt gtcacatata
     6361 aaaattaagc tgatgacttt gcagtgactc aagttgtctc tttatcatgg tttaccaggt
     6421 agagtgcctg gctattacta tataatgaag cccactggct tgacttgtaa gttcaaccta
     6481 aaccacaatc ctagaccatc atggatttag gagtagattc ttcttgaaat cccacatcca
     6541 gaaactagac attagaatgt tgaggcagtt tcccagagaa acaagcatat tgcctcatgg
     6601 atgaaagact tgtagttcta gtttcagtga cttgttatat ctacttacat acaacaggga
     6661 ggcaagagga ttctctgtca tctctggtga ctgagtgtaa aatatgtgcc aagtctgcag
     6721 cacagtgacc aaatctgaca atcgagctct ggatcaccac ttgattatgt agtagactca
     6781 tttataaagc agcttaggaa ctaattaaac atggaggatg aattaccttc ctatcccttg
     6841 agataagaca tctttcagtt tcatgattaa ggattgttgc tgttttatag ttactctgtt
     6901 catcacagtg taaatggtga tgcgtgtcgt aggtgtgcag ctatttgagg gactaaggga
     6961 tggagatatt ctgtcaaatg aatctcttca gtataccagt ttgtgggagg gatatgagac
     7021 atgtggatgg cagtgagaga tcgtgcctct agatcttgat ggaggcttgg tgagacacac
     7081 ttaaataagc acgtggaggt tagaatagag ggcagagtaa aaggaagctc catctgagca
     7141 agtacaccaa atgatctcag ccctgcaact tgacccaggt agggccacca ctacgccttc
     7201 acttgtcacc caagctccaa ccacagagag tttgacaagt ttgtgttatg atgttggctt
     7261 ggctttgtat ttttaattaa ctttggattt ttagtggttt tgtcatataa ctgtctgagt
     7321 ttggtaggta ggattacttt gaaaagggtt tactagtgtg gtcctccggg tagaatttag
     7381 ctgtaagcat gttgttagcc agcctgtaga ctgttaatta cttaataatc tcattgggaa
     7441 aatactagta gttttatatt tggatgacat aattggaaaa gcagattagc tgctactact
     7501 tttaaaagac ttaaggtcgg gatgcctttt tttccatgta aggaaatgaa aagaccaaaa
     7561 tcttcaggca aaaagcaagt tgcaaaatta gaaaccattg gctaaaaatg tgttttgttg
     7621 agtttccaaa tggatgaatt ttcatttgga cattacatca ctaaattcat tagattttgt
     7681 ctgcattgga aagatactct tctagcatat ctttcccaaa gatatctaat ttggattctg
     7741 tttcatgcaa atttgcatcc cggaggttga agttggagtt tgaggttgga aaatatcttt
     7801 gaaggcagaa tcagttgagt tgtgagggtg aagcctcaca tacttctcaa cagacatgat
     7861 aaaattcacc tgcatgagtt ggcaggtggg agaaccaaac tggatcactg ggtaagacta
     7921 ctcagtaaag caatgaactg cttgcttaga gaagcatcac tatccccatt gagaaaaatg
     7981 tgtggcaaga tgatacagct acacagtatc aaatgaatgg gtcaattcag cacccccaaa
     8041 tttaattctg tggggaaaaa ttattgagcc agttgtcagt gttctgttac atgactggca
     8101 gactaaattc ttcatcgttg ttgttattgt tgttgttgtt tctcattttc actcgcacgg
     8161 ccttattctc ataattaaaa tctaattcat tttctcttta gtgttagtag actccaacaa
     8221 cagaagtggc atctgtgtat tcataatcag catttaccct ggcaggagac taatcagata
     8281 ggccggtctc agacattaat cctaccatct gatatttttg gtgaaggaaa aagtattaat
     8341 tctctttcca tcctcctcct cagaaatata gaagccctct ttaccaaaat catcacattt
     8401 tactctgtaa tctaccagct aaaagaaaat tgcattgtgg ggagggcaga aagggggtgg
     8461 gggtgggggc gggggggtgg ggggtgggga agccccacaa agccagattg cagttcttgc
     8521 ccctttttgc gtctgacatg agatgttaaa gaattattca tagtgctcac attgggttag
     8581 gggacactga actgcttttt agatccatga tcagtcatca ttcttctaag agattggagc
     8641 tttgctgttt cattaactgt gcagtgtaga ctaatggtgt ttaataaaaa tcattcaaaa
     8701 tttcaaactc ttttgccagt gacctcaatt ttgttggctc tgtgatttgt atcagacttt
     8761 gaggagggaa gggggaagtg aaggaagcct acgtccaggc ccctgacagg atgctgcagt
     8821 agcaagctca agctcgcctg cctgccagca gttgctggtg agcagcagca tgcagaccag
     8881 ctgtgggaag cctcctgaag aatgccccag ctgatgcttt cagctgggaa tagtttgttc
     8941 ctattgggga actcattgtt ctccagtctc tgcagcagga agccagctgt catattcgga
     9001 gggaatttca gatgctttac ctttttggtt ttgtcctgca tcactcatgt ggctacgaaa
     9061 gtgtctctga gaatagagcc caatgtggtg acaatgggta gtcaaatgca ccccagatgc
     9121 tcaagccctg ttgtggttct gcagtgttta tgaaattggg aggaaggaga ccctggacag
     9181 taagcaaaat tggagacact ccaacgaggc taagttaatg ccgtgttgcc cagaacaaga
     9241 tctagcttct catttggtca gcctagcatg caaccagtgg tgtgctggta aaatgtttaa
     9301 caaccagctc gctgagaata gaaagcacct ggtttgcacc atttgccaat ttccatggca
     9361 taaatactac cactttagat gattttaagc taccaactgt gatgtcactg aacacatggt
     9421 tggaaagaga tgcacgcagt tggctcttgc aagcctgggc aaaaatgctt caacacgcca
     9481 ctggatgcag ccagtcagag ggttcatatt taatatatgt gttcatgtgg acacacacag
     9541 acacacacac acaaactcac ccttacacac acacttcgat gactaaaaca attacatagt
     9601 tttaagatat gaatcaatgt gtgaatgtag aaagcttatg ataaggccct agaggtatgg
     9661 gttgccctgg aagcctaggt tttaagcagg agaatagctg agaagaatga agccctcctg
     9721 agctgaaagg agagatggat caatggagat ggttccatca tctccttcca tatctcacag
     9781 gtaaaatggg cactcagaaa accctcacga ttgatttttt aaaaagataa gtgagtgttt
     9841 tttattttat tattattgtc atcattattt tgatttacaa atgctatttg taacttttac
     9901 atgtaactag gataaagtat ttacgggaac tctatggaga atagcacaat ccagaattta
     9961 ctgtgttttt cttttatgtg acgtggaaac tcagtaattc tcccaccttc acattgttgt
    10021 tcataagaat tttactttag ttattaggga atctaagttt tttgttaaca tttgttttta
    10081 gttaaaagta tctacttact gttttagctc tgaactcaaa ccagaatatc tctgtatcaa
    10141 ttgcatgact attcagaaac aataatccaa accaaaataa ttctttttcc acccagtacg
    10201 aagaaaacta agctcagtaa caagaaggca taaactaaag tatataatga ggctttcatt
    10261 aaatacacac acacacacac tcacacacac acacatacac tttttaaatt tttaaattag
    10321 gcctccacac ataaatcatt ttgaaagtag aatagaaaat ctcaaagaat tcattctcct
    10381 ggtcctgtgc atcttctgca gttaataaga ggtttgtatc tggaaagatg gaagaacttg
    10441 ttctaaaatc ttatttttca aaaaaaaatt tccattttct ctctgggcct gtatccatgg
    10501 ttgaatgtta gccctggagg agatccatgt cttactcgct ctttctggcc cttctgtctt
    10561 ttgcctctgc aattcttttt gtagctggca cgatagcagg gactgggggt ctatcctttc
    10621 atggtattgc tacaatattt gtccttactg gaaaatggta acatccgggt ctgatttaat
    10681 tggcattaca cttacacagg gactctgagc acccccgtca ccacaccaga cagtggacca
    10741 gttttcacag ctacaaagag ctagaaatgt gtttaacatc atccagtgca tcccctaatt
    10801 caaaaccatc ctcactaatc aatcatattc acccataaat attacaaatg agattgattc
    10861 catctcaaga caatttgtca aatacttaat tttcttcctg gatgattcta cttactggat
    10921 attttagaaa gagaaatgtc tgagataaaa tccctcacat ttactcaata taacaaatta
    10981 ctgtttctac tcctattctg agtagtgctt ctgaagattg tttgctgtag tgttgtcttt
    11041 gataaaatga atgtcagtag tgagcctttt agagatacca tgctcagaaa tcctctttgg
    11101 gatcagaaga tacctaaaat tctccccttt tgcccacttg gttagatgag tgatatattc
    11161 tttggatcct gcaaagaaga gattggttta ttttcttttc tggtggtggt agtggttgta
    11221 tctgtggctg tgatggttgt tgttacttgt ctctctctct ctctggctct ggcttttgct
    11281 ttcctgctag tgttctttct ctttccaaac aaatagttaa attaaatgtg agcttctgaa
    11341 ttgtacttgt tcatactttc aaaacataac agattaataa aaatagatgt gtcctgattt
    11401 aaaacatgcc ccctggaaag gcatgctgta ttatgaaatc gtgataatat aactgcatta
    11461 ttacatggca gtataaatat tagtctgttg aattcatttg tccaattgta taactttgtg
    11521 gagcagtgtt ttgacctttg atacataatt ctggagcaag tggagtggtt gcaggcagat
    11581 gagacagtgt tatatcagga tttttcaatc aactttagtt ggaggcctgg caattacaaa
    11641 catcttcaga tgtttctgta accattataa atatgaaaaa aacctcttca aaaaatttcc
    11701 catagtactt cagtcaagac tttttaggtt tatctttttt ttttcatttc tccttttcct
    11761 tttccattat ttttcgatgg gggggttgtt atcattgact gaagaaatat tttgattgca
    11821 atggtctctc tctctctccc cctctctctc tctctcctct attctttcct ccttccctct
    11881 gtccatcacc cctcattaaa atattgaaat ctggagtctt tgataaatct gcattagacc
    11941 aggctatatg ctaggaatga aatctgggca aatatcgatg ggttttcaaa gaatgctcca
    12001 tgttcattgg gccctttcac accccacagt gataaatgaa aaggatagag gtagtttttt
    12061 caaaagagca ctttaataat atcctctgag acctaatgca gtttaacaaa tgactccacc
    12121 tatttttcca gtaggtaaat tgactgagac ttgcaaaata cccctgagag ttgtcagggg
    12181 tgtcttctgc ctggtctata gcgtgtgtgt ttgctttgta tctaacaggc acattcacgt
    12241 ctcgtgtact catatgaagt atttcctaac attcccatta gcctgtatat aagaatcaga
    12301 aagataatcc caacatgttg taaatgaaga tgtgactcta taacctttct cttcttcctg
    12361 gaaaaaaaag gacattttca tgcatatttt aaacagaaat tttgtatatt taagtgtcat
    12421 agaaaatatt tattgagtaa ctgggacaca aatgggaatt taattgtcat catatgcttt
    12481 gtgtgtgggg atgcttacca acaccatgtc gctggaccat tgtggcaagc cataactgca
    12541 caaagagtac acatcgtcag tgtgtgtgtg tgtgtgtgtg tgcgcgcaca cacgtgcgtg
    12601 tgtgtgtccc tgcatgtgca acatgtctag cttgctgtcc ttcatgggat tttagctttc
    12661 ccttcttgaa aaacattatt ttacagttcc aggaggccct ggttacatta ctatatgaag
    12721 gcagtgattt gaaatgaaaa ttcctttcct cttggaagct ttggtcataa tatcatggtt
    12781 caattaaacg gattccaccg gactttgtga tgaaaaaggc tctgttaaaa tccaattgag
    12841 tttccaagag gaaattgtag taggtcaaga tgcatgagag ggaagatgga ggccacctca
    12901 actggagaac atgagctgag ttgagccctc agtgttgaag ttgacttgct ccaagctgca
    12961 gtctaaaacc ctggggcccg tgcctggcct atgctccctc ccaagtaagt agaggagcag
    13021 aaccatcagg aacagcctgc ctggctccta tgaagaaaac ttcctgacgt cctgtcccca
    13081 aaggaagacc ctttccccaa gggcacccca ggtggccatt aaattgtgat gatcattcag
    13141 aaagtgcccc cttggcttta tgagaatcca attagtcttc tgaaccacct tttcttgggt
    13201 gcagatttcc aacattcatg ctcattgcag atccaccaac tgtcactgtt cttaacaagc
    13261 atgctcgtct tgtcagaatt tcagtaagtt ccaatttcct gtacagacca gggtaaactg
    13321 ttctaaaatc aatcaattaa tgaaatgtta tctggttttt aaaagctggt ttcatgtgct
    13381 ttatgtgtat aaaactatat ctgcctgtgt ggctttgcat ttcaaatgtg tggcgcacaa
    13441 gcgttttgtt ggtgctttgt tctcagtaca gtaactctgt gtacaaacat tttaatgtgg
    13501 ttttgttgtt ttccaacaag atgtctctgt aaaaatgata ttggctgagc tggtgcgttg
    13561 gtttctctca tagaggcatt aactatactg ccaatgcatt gaattattta aaaatgcaaa
    13621 ataaaatttt tatgaaaatc tca
//
