LOCUS       NM_001166693            9261 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens ALF transcription elongation factor 1 (AFF1),
            transcript variant 1, mRNA.
ACCESSION   NM_001166693
VERSION     NM_001166693.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Tretti Parenzan,C., Molin,A.D., Longo,G., Gaffo,E., Buratin,A.,
            Cani,A., Boldrin,E., Serafin,V., Guglielmelli,P., Vannucchi,A.M.,
            Cazzaniga,G., Biondi,A., Locatelli,F., Meyer,L.H., Buldini,B., Te
            Kronnie,G., Bresolin,S. and Bortoluzzi,S.
  TITLE     Functional relevance of circRNA aberrant expression in pediatric
            acute leukemia with KMT2A::AFF1 fusion
  JOURNAL   Blood Adv 8 (5), 1305-1319 (2024)
   PUBMED   38029383
  REMARK    GeneRIF: Functional relevance of circRNA aberrant expression in
            pediatric acute leukemia with KMT2A::AFF1 fusion.
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Che,Z., Liu,X., Dai,Q., Fang,K., Guo,C., Yue,J., Fang,H., Xie,P.,
            Luo,Z. and Lin,C.
  TITLE     Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in
            regulating RNA polymerase II transcription elongation
  JOURNAL   J Mol Cell Biol 15 (8) (2024)
   PUBMED   37528066
  REMARK    GeneRIF: Distinct roles of two SEC scaffold proteins, AFF1 and
            AFF4, in regulating RNA polymerase II transcription elongation.
REFERENCE   3  (bases 1 to 9261)
  AUTHORS   Mu,J., Xu,Y. and Zhu,H.
  TITLE     [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms are
            correlated with disease susceptibility and immune infiltration in
            ankylosing spondylitis]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (5), 741-748 (2023)
   PUBMED   37313815
  REMARK    GeneRIF: [AF4/FMR2 and IL-10 gene single nucleotide polymorphisms
            are correlated with disease susceptibility and immune infiltration
            in ankylosing spondylitis].
REFERENCE   4  (bases 1 to 9261)
  AUTHORS   Liu,H., Westergard,T.D., Cashen,A., Piwnica-Worms,D.R., Kunkle,L.,
            Vij,R., Pham,C.G., DiPersio,J., Cheng,E.H. and Hsieh,J.J.
  TITLE     Proteasome inhibitors evoke latent tumor suppression programs in
            pro-B MLL leukemias through MLL-AF4
  JOURNAL   Cancer Cell 25 (4), 530-542 (2014)
   PUBMED   24735925
REFERENCE   5  (bases 1 to 9261)
  AUTHORS   Leach,B.I., Kuntimaddi,A., Schmidt,C.R., Cierpicki,T., Johnson,S.A.
            and Bushweller,J.H.
  TITLE     Leukemia fusion target AF9 is an intrinsically disordered
            transcriptional regulator that recruits multiple partners via
            coupled folding and binding
  JOURNAL   Structure 21 (1), 176-183 (2013)
   PUBMED   23260655
  REMARK    GeneRIF: The structure of AF9 in complex with elongation factor AF4
            reveals that aliphatic residues form an integral part of the
            hydrophobic core of the complex.
REFERENCE   6  (bases 1 to 9261)
  AUTHORS   Krivtsov,A.V., Feng,Z., Lemieux,M.E., Faber,J., Vempati,S.,
            Sinha,A.U., Xia,X., Jesneck,J., Bracken,A.P., Silverman,L.B.,
            Kutok,J.L., Kung,A.L. and Armstrong,S.A.
  TITLE     H3K79 methylation profiles define murine and human MLL-AF4
            leukemias
  JOURNAL   Cancer Cell 14 (5), 355-368 (2008)
   PUBMED   18977325
  REMARK    GeneRIF: Conditional expression of an Mll-AF4 fusion oncogene
            induces B precursor acute lymphoblastic and acute myeloid leukemias
REFERENCE   7  (bases 1 to 9261)
  AUTHORS   Chen,C.S., Hilden,J.M., Frestedt,J., Domer,P.H., Moore,R.,
            Korsmeyer,S.J. and Kersey,J.H.
  TITLE     The chromosome 4q21 gene (AF-4/FEL) is widely expressed in normal
            tissues and shows breakpoint diversity in t(4;11)(q21;q23) acute
            leukemia
  JOURNAL   Blood 82 (4), 1080-1085 (1993)
   PUBMED   8353274
REFERENCE   8  (bases 1 to 9261)
  AUTHORS   Domer,P.H., Fakharzadeh,S.S., Chen,C.S., Jockel,J., Johansen,L.,
            Silverman,G.A., Kersey,J.H. and Korsmeyer,S.J.
  TITLE     Acute mixed-lineage leukemia t(4;11)(q21;q23) generates an MLL-AF4
            fusion product
  JOURNAL   Proc Natl Acad Sci U S A 90 (16), 7884-7888 (1993)
   PUBMED   7689231
REFERENCE   9  (bases 1 to 9261)
  AUTHORS   Morrissey,J., Tkachuk,D.C., Milatovich,A., Francke,U., Link,M. and
            Cleary,M.L.
  TITLE     A serine/proline-rich protein is fused to HRX in t(4;11) acute
            leukemias
  JOURNAL   Blood 81 (5), 1124-1131 (1993)
   PUBMED   8443374
REFERENCE   10 (bases 1 to 9261)
  AUTHORS   Gu,Y., Nakamura,T., Alder,H., Prasad,R., Canaani,O., Cimino,G.,
            Croce,C.M. and Canaani,E.
  TITLE     The t(4;11) chromosome translocation of human acute leukemias fuses
            the ALL-1 gene, related to Drosophila trithorax, to the AF-4 gene
  JOURNAL   Cell 71 (4), 701-708 (1992)
   PUBMED   1423625
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC093827.3, AC093779.3 and
            AC092658.2.
            
            On Nov 18, 2020 this sequence version replaced NM_001166693.2.
            
            Summary: This gene encodes a member of the AF4/ lymphoid nuclear
            protein related to the Fragile X E syndrome (FRAXE) family of
            proteins, which have been implicated in human childhood
            lymphoblastic leukemia, fragile chromosome X intellectual
            disability, and ataxia. It is the prevalent mixed-lineage leukemia
            fusion gene associated with spontaneous acute lymphoblastic
            leukemia. Members of this family have three conserved domains: an
            N-terminal homology domain, an AF4/ lymphoid nuclear protein
            domain, and a C-terminal homology domain. The protein functions as
            a regulator of RNA polymerase II-mediated transcription through
            elongation and chromatin remodeling functions. Through RNA
            interference screens, this gene has been shown to promote the
            expression of CD133, a plasma membrane glycoprotein required for
            leukemia cell survival. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK300364.1, SRR14038195.1036740.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000395146.9/ ENSP00000378578.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-230               AC093827.3         87880-88109
            231-304             AC093827.3         101367-101440
            305-425             AC093779.3         8772-8892
            426-1325            AC093779.3         9301-10200
            1326-1370           AC093779.3         46726-46770
            1371-1457           AC093779.3         52590-52676
            1458-1494           AC093779.3         54399-54435
            1495-1549           AC093779.3         57521-57575
            1550-1604           AC092658.2         10570-10624
            1605-1642           AC092658.2         10750-10787
            1643-1799           AC092658.2         13101-13257
            1800-2732           AC092658.2         19309-20241
            2733-2839           AC092658.2         29979-30085
            2840-3077           AC092658.2         31041-31278
            3078-3169           AC092658.2         31968-32059
            3170-3230           AC092658.2         32585-32645
            3231-3367           AC092658.2         36025-36161
            3368-3439           AC092658.2         36735-36806
            3440-3577           AC092658.2         37213-37350
            3578-3801           AC092658.2         39413-39636
            3802-9261           AC092658.2         40522-45981
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.3-q22.1"
     gene            1..9261
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /note="ALF transcription elongation factor 1"
                     /db_xref="GeneID:4299"
                     /db_xref="HGNC:HGNC:7135"
                     /db_xref="MIM:159557"
     exon            1..230
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            231..304
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..3923
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 2; AF4/FMR2 family
                     member 1; pre-B-cell monocytic leukemia partner 1;
                     proto-oncogene AF4; ALL1-fused gene from chromosome 4
                     protein; KMT2A/AFF1 fusion protein"
                     /codon_start=1
                     /product="AF4/FMR2 family member 1 isoform 1"
                     /protein_id="NP_001160165.1"
                     /db_xref="CCDS:CCDS54775.1"
                     /db_xref="GeneID:4299"
                     /db_xref="HGNC:HGNC:7135"
                     /db_xref="MIM:159557"
                     /translation="MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEK
                     IPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDASENRLGKPKYPLI
                     PDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGP
                     PDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPPL
                     SPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQP
                     PSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDL
                     KVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKF
                     PFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEK
                     PPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETP
                     APEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSES
                     KDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGS
                     RTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSS
                     LPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQED
                     SRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQP
                     PAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSK
                     TKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASSTKSNH
                     KDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQAD
                     LHMREAKKMKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETV
                     DLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHF
                     ESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTI
                     QNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYT
                     RQGFQQLQELTKTP"
     exon            305..425
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            426..1325
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1370
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1457
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1494
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1549
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1604
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1642
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1799
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..2732
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2839
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..3077
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3078..3169
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..3230
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3231..3367
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3439
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3440..3577
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3801
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     exon            3802..9261
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9241..9246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /note="hexamer: AATAAA"
     polyA_site      9261
                     /gene="AFF1"
                     /gene_synonym="AF4; FEL; MLLT2; PBM1"
                     /note="major polyA site"
ORIGIN      
        1 agccagctag cgagcgacgg gcgcgcgcgg cccctgcgca ctcggccgcc gcctgcgcgc
       61 gttgcggccg cagctgcacc tttgcctccg cggttccgca gccgagcccc gggaggaggc
      121 gctcccctgc gtcgtccgca cttgcgccct ttcccaccgc aacaggtcag gggagggcgg
      181 aggtgacgag ggcgcccgac ccgggactag aggacggaag ggtctggaag atgaacagac
      241 tagccacttt gcattgactg gaaacaatgg catttacaga aagagtcaac agcagtggca
      301 acagtttgta caatgacgac agaaacctgc ttcgaattag agagaaggaa agacgcaacc
      361 aggaagccca ccaagagaaa gaggcatttc ctgaaaagat tccccttttt ggagagccct
      421 acaagacagc aaaaggtgat gagctgtcta gtcgaataca gaacatgttg ggaaactacg
      481 aagaagtgaa ggagttcctt agtactaagt ctcacactca tcgcctggat gcttctgaaa
      541 ataggttggg aaagccgaaa tatcctttaa ttcctgacaa agggagcagc attccatcca
      601 gctccttcca cactagtgtc caccaccagt ccattcacac tcctgcgtct ggaccacttt
      661 ctgttggcaa cattagccac aatccaaaga tggcgcagcc aagaactgaa ccaatgccaa
      721 gtctccatgc caaaagctgc ggcccaccgg acagccagca cctgacccag gatcgccttg
      781 gtcaggaggg gttcggctct agtcatcaca agaaaggtga ccgaagagct gacggagacc
      841 actgtgcttc ggtgacagat tcggctccag agagggagct ttctccctta atctctttgc
      901 cttccccagt tccccctttg tcacctatac attccaacca gcaaactctt ccccggacgc
      961 aaggaagcag caaggttcat ggcagcagca ataacagtaa aggctattgc ccagccaaat
     1021 ctcccaagga cctagcagtg aaagtccatg ataaagagac ccctcaagac agtttggtgg
     1081 cccctgccca gccgccttct cagacatttc cacctccctc cctcccctca aaaagtgttg
     1141 caatgcagca gaagcccacg gcttatgtcc ggcccatgga tggtcaagat caggccccta
     1201 gtgaatcccc tgaactgaaa ccactgccgg aggactatcg acagcagacc tttgaaaaaa
     1261 cagacttgaa agtgcctgcc aaagccaagc tcaccaaact gaagatgcct tctcagtcag
     1321 ttgagcagac ctactccaat gaagtccatt gtgttgaaga gattctgaag gaaatgaccc
     1381 attcatggcc gcctcctttg acagcaatac atacgcctag tacagctgag ccatccaagt
     1441 ttcctttccc tacaaaggac tctcagcatg tcagttctgt aacccaaaac caaaaacaat
     1501 atgatacatc ttcaaaaact cactcaaatt ctcagcaagg aacgtcatcc atgctcgaag
     1561 acgaccttca gctcagtgac agtgaggaca gtgacagtga acaaacccca gagaagcctc
     1621 cctcctcatc tgcacctcca agtgctccac agtcccttcc agaaccagtg gcatcagcac
     1681 attccagcag tgcagagtca gaaagcacca gtgactcaga cagttcctca gactcagaga
     1741 gcgagagcag ttcaagtgac agcgaagaaa atgagcccct agaaacccca gctccggagc
     1801 ctgagcctcc aacaacaaac aaatggcagc tggacaactg gctgaccaaa gtcagccagc
     1861 cagctgcgcc accagagggc cccaggagca cagagccccc acggcggcac ccagagagta
     1921 agggcagcag cgacagtgcc acgagtcagg agcattctga atccaaagat cctcccccta
     1981 aaagctccag caaagccccc cgggccccac ccgaagcccc ccaccccgga aagaggagct
     2041 gtcagaagtc tccggcacag caggagcccc cacaaaggca aaccgttgga accaaacaac
     2101 ccaaaaaacc tgtcaaggcc tctgcccggg caggttcacg gaccagcctg cagggggaaa
     2161 gggagccagg gcttcttccc tatggctccc gagaccagac ttccaaagac aagcccaagg
     2221 tgaagacgaa aggacggccc cgggccgcag caagcaacga acccaagcca gcagtgcccc
     2281 cctccagtga gaagaagaag cacaagagct ccctccctgc cccctctaag gctctctcag
     2341 gcccagaacc cgcgaaggac aatgtggagg acaggacccc tgagcacttt gctcttgttc
     2401 ccctgactga gagccagggc ccaccccaca gtggcagcgg cagcaggact agtggctgcc
     2461 gccaagccgt ggtggtccag gaggacagcc gcaaagacag actcccattg cctttgagag
     2521 acaccaagct gctctcaccg ctcagggaca ctcctccccc acaaagcttg atggtgaaga
     2581 tcaccctaga cctgctctct cggatacccc agcctcccgg gaaggggagc cgccagagga
     2641 aagcagaaga taaacagccg cccgcaggga agaagcacag ctctgagaag aggagctcag
     2701 acagctcaag caagttggcc aaaaagagaa agggtgaagc agaaagagac tgtgataaca
     2761 agaaaatcag actggagaag gaaatcaaat cacagtcatc ttcatcttca tcctcccaca
     2821 aagaatcttc taaaacaaag ccctccaggc cctcctcaca gtcctcaaag aaggaaatgc
     2881 tccccccgcc acccgtgtcc tcgtcctccc agaagccagc caagcctgca cttaagaggt
     2941 caaggcggga agcagacacc tgtggccagg accctcccaa aagtgccagc agtaccaaga
     3001 gcaaccacaa agactcttcc attcccaagc agagaagagt agaggggaag ggctccagaa
     3061 gctcctcgga gcacaagggt tcttccggag atactgcaaa tccttttcca gtgccttctt
     3121 tgccaaatgg taactctaaa ccagggaagc ctcaagtgaa gtttgacaaa caacaagcag
     3181 accttcacat gagggaggca aaaaagatga agcagaaagc agagttaatg acggacaggg
     3241 ttggaaaggc ttttaagtac ctggaagccg tcttgtcctt cattgagtgc ggaattgcca
     3301 cagagtctga aagccagtca tccaagtcag cttactctgt ctactcagaa actgtagatc
     3361 tcattaaatt cataatgtca ttaaaatcct tctcagatgc cacagcgcca acacaagaga
     3421 aaatatttgc tgttttatgc atgcgttgcc agtccatttt gaacatggcg atgtttcgtt
     3481 gtaaaaaaga catagcaata aagtattctc gtactcttaa taaacacttc gagagttctt
     3541 ccaaagtcgc ccaggcacct tctccatgca ttgcaagaag cacaggcaca ccatcccctc
     3601 tttccccaat gccttctcct gccagctccg tagggtccca gtcaagtgct ggcagtgtgg
     3661 ggagcagtgg ggtggctgcc actatcagca ccccagtcac catccagaat atgacatctt
     3721 cctatgtcac catcacatcc catgttctta ccgcctttga cctttgggaa caggccgagg
     3781 ccctcacgag gaagaataaa gaattctttg ctcggctcag cacaaatgtg tgcaccttgg
     3841 ccctcaacag cagtttggtg gacctggtgc actatacacg acagggtttt cagcagctac
     3901 aagaattaac caaaacacct taatggagcc ccaggttgat tcaatgcctt gggaactatt
     3961 tttgcacatt ggaagcctca aaaacagtcc agacatttgt ttcatcagga caccaaactc
     4021 taaaaaagaa gcaccacgag atggccagga catttgtcca cttaaactct caacaacagt
     4081 gtgatcattg gttggacact gtggttatgc agaagcagag atgaggaggc tggccccaga
     4141 gatgatcttg cccttcctaa ctaaaggaca gaagtgcaat ttagcttaaa tgggtgtatg
     4201 aatggtctag aaacatttct attttttttt taaaccagca ggatacaagt tgcaaatgaa
     4261 atgaggagaa acagtttcaa ctctgaaagt gaatttcacg tcatctcagt agccacgcta
     4321 gtccattccc agaaggaaat tttttttttt aacaatgact tttggtaaag ggttttgtgg
     4381 atgatttttt ttcttttgag ttttgggaga aatatttgtt tagtaacttc taatggccat
     4441 ctgtaaacca taagtaatga aggactccac tgtgccccac tttctgccaa tgaacagtgg
     4501 cttgataata ccaagtattg ttgtaattta taaaattgaa ggcaaccccc gctcctgccg
     4561 cccccaatct ccccattgcc tagagcgctg cacattgacc ccagctctga cttctcatta
     4621 ctgtgctgaa agtcagccca cgtcggagcg gtgaggagga gccacagcac atggggtgcc
     4681 acctcgaggt ctgcacagga ggacttggcg ctgccatttc ctacccctgc catttcccac
     4741 ccctgcttca gcgaaaggga ctctctaaca gggcagtcac tgttgactct attctgaatt
     4801 tcctcccttg gggaagaagg gaaccaacat ttatacctga ccagatggct aaagtgcttt
     4861 taaagttttg tttaagtaga gctggaattt gaggtgctga tctgtggtct acagttatgt
     4921 ggtaactcat gttgtccagc caactcagag tttcgtcagt gaacaagaaa catgaaatct
     4981 gcttcttaga gaggctatat ttttctgcta caaatatttt atatttatag caaaactaga
     5041 ctttcagagt ccttgattgt ctaggggaag ttaactccct gagaggatgt agagatttgg
     5101 ggtggttgat tagacttttg aaaaactcat caccacatgc cttcactcca gagtgttctc
     5161 agctagattt gatttggttg aggaggaact gtggccctcc gtaagttatt gccatagtgt
     5221 atgcattaaa ccaagtccat tttgaatgac ctaaaatgaa gtaacacaat cagaaatccc
     5281 atgtgcccat aagcacagat ttttcttttt cattgaaact ttaaaggtta ttattggaaa
     5341 cattactttg agtgcagtgt ttttaaaagc caattctttt ttatcccttt tagaagtaga
     5401 atttgcacac ttactacaat tgaggagtgt catctctata actttttctc cgcctttgtc
     5461 ccattctgcc cctgtacatg tttcctacca agcatgtttc acattttcct attagtggag
     5521 gagggagaac catatttatt tataatgaag acatctaaga tccctatgat gaatgcagga
     5581 actctcttgg tagtttgtaa atacacaaag ggatgtgtcg agggatggga gcgatgctta
     5641 tctctcacag tgtgagtggt ctgtgtgagg ctgttccttc agtttcttct ccagactgtt
     5701 ctttggttgt cactaagtca gaggtctggt ccctcatgtt taggtgaaag ccagagaatg
     5761 acagctgtag tcatatctga gcataagacc ttgatgtgtg attcctgatg accggtttca
     5821 tttattcatg ttataaagca aagccctggt cctttttaaa ctactagttt taaaaacctg
     5881 tgttaaatga acagtaattg cctggtaggt ttggtgtgtg tgtagcattg tgtgtccatc
     5941 tgttatatgt aaaggacaag gcaccagaat caggctttat ttcgatattg aagatgttat
     6001 ttaacatctt tcttttttcc ttactccctt agccatcccc tccccttttg tcctatcatt
     6061 ccctagaaca agccacctgt caattgtgaa gggttgtgtt ctttatggca ggttctatgc
     6121 agattgtgcc agagcatgtg cgtgttctgt tggcaagcca cagtgctccc ttgactgaag
     6181 acatttccag gtagatttct cagccagctc taaaacagat tgctttttca gtggccttac
     6241 tctttgtggg tttttttttt tttctctgaa cttgatataa agattttatt tgtcccttga
     6301 aaaagtaaca aatgtgcata gatcaatttg tactactttg gtcattggat atttctgatc
     6361 cttattgcat tgtacctaaa ggagagtaac taatggtaac ctttttaata gagtatgtga
     6421 aaggtagtgg ctgatgaatc cttaacgttc atagggtctt tttgctgtta cggttgtata
     6481 tagaggtctg aaggattttt aaaatgattt gcactttttc actgcatgct tacaattccc
     6541 aaaggcaaaa tctgtactga ggtagatcat ttgaaagggc tagattataa aattaagcct
     6601 tagagtatgg aaagttctta taacaataat agtacacact tcagagtaag acaaatgcaa
     6661 agcatcttaa ggagtgaaaa tagagtgtaa atcttgcctt tggcactaca aggtgtgtgt
     6721 gtgtgtgtgt gtgtgtgtgt gtgtctttag taggaaatgg aagaacactg ttttattttt
     6781 taaagtgttt aatgtttctg tcctttctgt gaattattga atttaagagc cctgctaaat
     6841 aatgaaaaaa cactttacta aaatttatca aattatactg ggttcggatt gtgaaaacat
     6901 tggccaccta gtagcagtgg tgaggagtgg gagggcccag caagcattta tcagaaatag
     6961 aatcacaata ggaggagaat ttgactgtct gatattatga tttgattaca atactgaatg
     7021 ggaaaagtat ctaatatttt gtaacaaaaa gaccttcata ttatctgttt tgaccaaaat
     7081 atgtagctat ttcccttaca cagattggac cgcacttatc tcccttgtcc tgtatccttt
     7141 aatttcaggt ctcaggatgt ttagaaagct aaaaccccct acccctttct ggctgaaaac
     7201 ttgccttatt tggtatctta cacattaatg ttactagcat caggagctta ctgttttatt
     7261 atgattcatc ttcagtaatt tttagaagca agaagaaagc cattgtgtcc tctacaatta
     7321 acaaaactta tctctgatat acaaagggat ataaatatat acacttaaat agagaaaaag
     7381 aggttgattg aattgtgcct ttgagtgaac ccagttttta aataccgctg tgtttgtttc
     7441 gccatggctt cagggatgct acatggctct tgcacctttt actcctctgc tttatgaagt
     7501 ttgagttgta tttgtgcatc ttaaagtagg ttgaggcttg aggctgggct ttcgggtttt
     7561 tttgtttttt gttttgtttt gttttgtttt gttttcttgt acttaaacct gcttgcttcc
     7621 taccacagat tctttatttt cccaaacact acaaaaaaac ttttaaaact ttgccatttc
     7681 atctgtttac actctttgcc actgattagc agtatttaaa tcttgcaaga atattttgtg
     7741 ctttctttag aaacacaaga gtatagattt ttctcactga aaagtgagag ttacgcattg
     7801 cagccatgaa gggatgctag gatcaattat ggcagtacct tttttcccct cctgttcttg
     7861 agccagttgt ctcttttgtt ttgggtccca cttaggatta atggatgtaa ggtattttcc
     7921 tgtgccttta ttttgtgtca ttctattgga aggaggtgta acggcagaat agcatcgtgt
     7981 tgggggtttt ccttcaaaca ctgcaagtga tattgccacc atgtgaacct caaatatgca
     8041 atccagttgt gttggtttct cggtgacttg gagtgttcat ctcttcatga attgtgagca
     8101 ctgaccatgt tcttcagttc ttaattatgg tgagttgaca aataccaact actgcttttc
     8161 tttaggtggc tataaatttc ttactgtcag gaggaaatga cattatattc tgttccactg
     8221 aacgtcagag atcagcaggc actgtactgg gtagagaagt gcctatactt ctctacctaa
     8281 gagggcagga gggaaaccct acagctcctt gtgagcctat atattagtat atcggcctgg
     8341 agaggacaag ggaataagac cactcatagt gaggctggcc aagctgcact ggtcggacca
     8401 ggcagtggct gacctaagga agacaacttg ctttgcttaa aagtagattt tttaagcaat
     8461 gcttaacaca ggcagcattc acctttgttc aggccatcga catgtattgt taaaattact
     8521 gcatatcccc ctcagatatc aagtatacac tgttcatgtt ggggttgtgt gtgtgtatgt
     8581 gtgtatgtac gcacgcatgt gtcccaaatc ttgttttaat tttttttttc tgaatgtgat
     8641 catgttttgg atgatacctg agcagggttg cctttttttt atttattacc attatatatt
     8701 atattatatt atattatata ttttttgctt tcttataact ttggaggaaa gtcaaatctt
     8761 ggtattatta aaattgtttt aaaaaggagt aaattttcca gttgataaat gaaaatcact
     8821 ggcctatgtt taataagttt ttctttaatt actgtggaat aacgtgccag ctatcatcaa
     8881 cacaatgatt ttgtacatag ggtagggaag cagtgatgct ctcaatggga agatgtgcaa
     8941 cacaaattaa ggggaactcc atgtatttta cctacttcag caatggaact gcaacttggg
     9001 gctttgtgaa taaaatttag ctgccttgta tagtcgtttg aaagaatatg tgatctgtga
     9061 gagaattata gttttttttt agaagaaaaa tctgcaaaag atctttccaa agacaatgtg
     9121 ccacagatct tttgttctct gtaatgagga ttaattgctg tttaaacaaa aatgtaattg
     9181 ttcatcttta aattctttcc ttttcataag aggatcaagc tgtaaaaaaa caaaaaaatt
     9241 aataaaaatt tcgagaaatc a
//
