LOCUS       NM_001410951            7761 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens afadin, adherens junction formation factor (AFDN),
            transcript variant 9, mRNA.
ACCESSION   NM_001410951 XM_017010884
VERSION     NM_001410951.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Awotoye,W., Mossey,P.A., Hetmanski,J.B., Gowans,L.J.J.,
            Eshete,M.A., Adeyemo,W.L., Alade,A., Zeng,E., Adamson,O., James,O.,
            Fashina,A., Ogunlewe,M.O., Naicker,T., Adeleke,C., Busch,T., Li,M.,
            Petrin,A., Oladayo,A., Kayali,S., Olotu,J., Sule,V., Hassan,M.,
            Pape,J., Aladenika,E.T., Donkor,P., Arthur,F.K.N., Obiri-Yeboah,S.,
            Sabbah,D.K., Agbenorku,P., Ray,D., Plange-Rhule,G., Oti,A.A.,
            Albokhari,D., Sobreira,N., Dunnwald,M., Beaty,T.H., Taub,M.,
            Marazita,M.L., Adeyemo,A.A., Murray,J.C. and Butali,A.
  TITLE     Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft
            Lip With or Without Cleft Palate
  JOURNAL   Cleft Palate Craniofac J 61 (4), 697-705 (2024)
   PUBMED   36384317
  REMARK    GeneRIF: Damaging Mutations in AFDN Contribute to Risk of
            Nonsyndromic Cleft Lip With or Without Cleft Palate.
REFERENCE   2  (bases 1 to 7761)
  AUTHORS   Zhang,M.X., Shi,W.Z., Liu,J.X., Wang,C.J., Li,Y., Wang,W. and
            Jiang,B.
  TITLE     [Clinical characteristics and prognosis of MLL-AF6 positive
            patients with acute myeloid leukemia]
  JOURNAL   Beijing Da Xue Xue Bao Yi Xue Ban 53 (5), 915-920 (2021)
   PUBMED   34650294
  REMARK    GeneRIF: [Clinical characteristics and prognosis of MLL-AF6
            positive patients with acute myeloid leukemia].
REFERENCE   3  (bases 1 to 7761)
  AUTHORS   Baskaran,Y., Tay,F.P., Ng,E.Y.W., Swa,C.L.F., Wee,S., Gunaratne,J.
            and Manser,E.
  TITLE     Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions
  JOURNAL   Nat Commun 12 (1), 5315 (2021)
   PUBMED   34493720
  REMARK    GeneRIF: Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7761)
  AUTHORS   Lorger,M. and Moelling,K.
  TITLE     Regulation of epithelial wound closure and intercellular adhesion
            by interaction of AF6 with actin cytoskeleton
  JOURNAL   J Cell Sci 119 (Pt 16), 3385-3398 (2006)
   PUBMED   16882694
REFERENCE   5  (bases 1 to 7761)
  AUTHORS   Saito,S., Matsushima,M., Shirahama,S., Minaguchi,T., Kanamori,Y.,
            Minami,M. and Nakamura,Y.
  TITLE     Complete genomic structure DNA polymorphisms, and alternative
            splicing of the human AF-6 gene
  JOURNAL   DNA Res 5 (2), 115-120 (1998)
   PUBMED   9679199
REFERENCE   6  (bases 1 to 7761)
  AUTHORS   Mandai,K., Nakanishi,H., Satoh,A., Obaishi,H., Wada,M.,
            Nishioka,H., Itoh,M., Mizoguchi,A., Aoki,T., Fujimoto,T.,
            Matsuda,Y., Tsukita,S. and Takai,Y.
  TITLE     Afadin: A novel actin filament-binding protein with one PDZ domain
            localized at cadherin-based cell-to-cell adherens junction
  JOURNAL   J Cell Biol 139 (2), 517-528 (1997)
   PUBMED   9334353
  REMARK    Erratum:[J Cell Biol 1997 Nov 17;139(4):1060]
REFERENCE   7  (bases 1 to 7761)
  AUTHORS   Joh,T., Yamamoto,K., Kagami,Y., Kakuda,H., Sato,T., Yamamoto,T.,
            Takahashi,T., Ueda,R., Kaibuchi,K. and Seto,M.
  TITLE     Chimeric MLL products with a Ras binding cytoplasmic protein AF6
            involved in t(6;11) (q27;q23) leukemia localize in the nucleus
  JOURNAL   Oncogene 15 (14), 1681-1687 (1997)
   PUBMED   9349501
REFERENCE   8  (bases 1 to 7761)
  AUTHORS   Tanabe,S., Zeleznik-Le,N.J., Kobayashi,H., Vignon,C., Espinosa,R.
            3rd, LeBeau,M.M., Thirman,M.J. and Rowley,J.D.
  TITLE     Analysis of the t(6;11)(q27;q23) in leukemia shows a consistent
            breakpoint in AF6 in three patients and in the ML-2 cell line
  JOURNAL   Genes Chromosomes Cancer 15 (4), 206-216 (1996)
   PUBMED   8703846
REFERENCE   9  (bases 1 to 7761)
  AUTHORS   Saha,V., Lillington,D.M., Shelling,A.N., Chaplin,T., Yaspo,M.L.,
            Ganesan,T.S. and Young,B.D.
  TITLE     AF6 gene on chromosome band 6q27 maps distal to the minimal region
            of deletion in epithelial ovarian cancer
  JOURNAL   Genes Chromosomes Cancer 14 (3), 220-222 (1995)
   PUBMED   8589040
REFERENCE   10 (bases 1 to 7761)
  AUTHORS   Prasad,R., Gu,Y., Alder,H., Nakamura,T., Canaani,O., Saito,H.,
            Huebner,K., Gale,R.P., Nowell,P.C., Kuriyama,K. et al.
  TITLE     Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in
            acute myeloid leukemias with the t(6;11) chromosome translocation
  JOURNAL   Cancer Res 53 (23), 5624-5628 (1993)
   PUBMED   8242616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL009178.4, AL731868.5 and
            AL049698.3.
            
            On Aug 18, 2022 this sequence version replaced XM_017010884.2.
            
            Summary: This gene encodes a multi-domain protein involved in
            signaling and organization of cell junctions during embryogenesis.
            It has also been identified as the fusion partner of acute
            lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid
            leukemias with t(6;11)(q27;q23) translocation. Alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene, however, not all have been fully
            characterized.[provided by RefSeq, May 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.1801923.1,
                                           SRR14038193.3912682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               AL009178.4         12383-12709         c
            328-523             AL731868.5         26940-27135
            524-636             AL731868.5         32775-32887
            637-800             AL731868.5         34603-34766
            801-961             AL731868.5         37724-37884
            962-1119            AL731868.5         42749-42906
            1120-1231           AL731868.5         51604-51715
            1232-1399           AL731868.5         53251-53418
            1400-1494           AL049698.3         81361-81455         c
            1495-1757           AL049698.3         79866-80128         c
            1758-1827           AL049698.3         75947-76016         c
            1828-1946           AL049698.3         71044-71162         c
            1947-2008           AL049698.3         67171-67232         c
            2009-2214           AL049698.3         66844-67049         c
            2215-2235           AL049698.3         64910-64930         c
            2236-2381           AL049698.3         64020-64165         c
            2382-2476           AL049698.3         63595-63689         c
            2477-2742           AL049698.3         62900-63165         c
            2743-2886           AL049698.3         61101-61244         c
            2887-3085           AL049698.3         59400-59598         c
            3086-3189           AL049698.3         55374-55477         c
            3190-3276           AL049698.3         53242-53328         c
            3277-3342           AL049698.3         35139-35204         c
            3343-3416           AL049698.3         34858-34931         c
            3417-3535           AL049698.3         34274-34392         c
            3536-3730           AL049698.3         31432-31626         c
            3731-3822           AL049698.3         30389-30480         c
            3823-4008           AL049698.3         29855-30040         c
            4009-5010           AL049698.3         26146-27147         c
            5011-5145           AL049698.3         15766-15900         c
            5146-5434           AL049698.3         12288-12576         c
            5435-5519           AL049698.3         9135-9219           c
            5520-7761           AL049698.3         6310-8551           c
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..7761
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="afadin, adherens junction formation factor"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
     exon            1..327
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..5673
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="isoform 9 is encoded by transcript variant 9;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 4; myeloid/lymphoid
                     or mixed-lineage leukemia; translocated to, 4; ALL1-fused
                     gene from chromosome 6 protein; protein AF-6"
                     /codon_start=1
                     /product="afadin isoform 9"
                     /protein_id="NP_001397880.1"
                     /db_xref="CCDS:CCDS94035.1"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
                     /translation="MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMR
                     FYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGER
                     RLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRT
                     LSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNP
                     EVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADF
                     AVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPS
                     DKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSP
                     DGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRS
                     MDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPR
                     HKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQ
                     PDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLY
                     MACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAF
                     WMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDD
                     PEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDP
                     DSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIP
                     NINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQ
                     LEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIY
                     FEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVK
                     SVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIY
                     HGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEP
                     KKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTP
                     PPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQ
                     RVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKS
                     VTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDL
                     PLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQM
                     RTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSK
                     EELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKL
                     MLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEE
                     MRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLL
                     EPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYN
                     EEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFK
                     ERQRLFSQGQDVSNKVKASRKLTELENELNTK"
     exon            328..523
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            524..636
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            637..800
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            801..961
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1119
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1231
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1399
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1494
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1757
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1827
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1946
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2008
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2214
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2235
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2381
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2476
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2742
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2743..2886
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2887..3085
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3189
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3276
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3342
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3343..3416
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3417..3535
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3730
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3822
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3823..4008
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4009..5010
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5011..5145
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5146..5434
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5435..5519
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5520..7761
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7740..7745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="hexamer: ATTAAA"
     polyA_site      7761
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="major polyA site"
ORIGIN      
        1 gcacaccggc cggcggggct gaggcggcgc ggcgcggcgc ggactgagcc ccaggcggag
       61 gccaagtcgg aggacgcggc gcggccgcgg aggcggaggc agccgcggag gcggaggcgg
      121 ccggcggggg gtggcgaggg gcgccgggcc cccgcggacc tgtcgtcctc ggcccgtcct
      181 ccggccccgg ccccgcgcgg ctgaggaggc gcggccagga ccatgtcggc gggcggccgt
      241 gacgaggagc ggcggaagct ggccgacatc atccaccact ggaacgccaa ccggctggac
      301 ctgttcgaga tcagccagcc gaccgaggat ttggagttcc atggagtgat gagattttat
      361 tttcaagata aagctgctgg aaactttgca acaaaatgta ttcgggtctc tagtactgcc
      421 accactcaag atgtaatcga aacgctcgcg gagaaatttc gacctgatat gcgaatgctg
      481 tcctctccca agtattcact ctatgaagtg catgtcagcg gagaaagaag attggatata
      541 gatgagaaac ctctagttgt acaactgaat tggaacaaag atgatcggga aggcagattt
      601 gttcttaaga atgagaatga cgccattcct cctaagaagg ctcaaagtaa tggacctgaa
      661 aagcaggaaa aagaaggggt tatccagaac ttcaagagaa ctctctcaaa gaaagaaaag
      721 aaggaaaaaa agaagagaga aaaagaggca ttgcgacagg catctgataa agatgataga
      781 cctttccaag gggaggatgt tgaaaattct cgactggctg ctgaggttta caaagacatg
      841 ccggaaacca gctttactcg aaccatttct aatcctgagg tggttatgaa acgacggagg
      901 cagcaaaaat tggaaaagag aatgcaggaa tttcggagct cagatgggcg gcctgattca
      961 ggtggaacat tgagaattta tgcagatagt ttaaaaccaa atattcccta caagacaatc
     1021 ctgctgtcta ctacagatcc tgcagacttt gctgtggctg aagctttaga gaagtatggt
     1081 ctggaaaaag aaaaccctaa ggattactgc atcgcccggg ttatgcttcc tcctggagcc
     1141 cagcattctg atgaaaaggg tgctaaagaa attattcttg atgatgatga gtgtccttta
     1201 caaatcttca gggaatggcc aagtgacaaa gggattttag tctttcagtt gaagaggagg
     1261 ccaccagacc acatcccaaa gaaaaccaag aaacacttgg aaggcaagac acccaaggga
     1321 aaggagagag ctgacgggtc tggctatggc tccacccttc ctccggagaa gctgccctat
     1381 ttagtagagt taagcccaga tggttctgac tctagagata agccaaagct ttaccgcctt
     1441 cagttaagtg ttactgaagt tgggacagaa aagttggatg acaactctat ccagttgttt
     1501 ggcccaggaa ttcagcccca tcactgtgac cttaccaaca tggatggagt ggtcactgtg
     1561 acgcccagaa gtatggacgc agaaacctac gtggaaggcc agcgcatctc agaaaccacc
     1621 atgctgcaga gtggcatgaa agtgcagttt ggggcgtccc atgtatttaa gtttgtggac
     1681 cccagtcagg atcatgctct tgcaaaaaga tctgtggatg gaggcctgat ggttaagggc
     1741 ccaagacata aacctggaat tgttcaggag acaacttttg atttgggagg agatattcat
     1801 agtgggacag cattaccgac aagcaagagc accactaggc tggacagcga cagagtgtcg
     1861 tctgcctcta gcacagccga gcggggaatg gtgaagccga tgatcagagt agaacagcag
     1921 ccagattatc gcaggcaaga aagcagaaca caggatgctt ctgggcctga gctgatacta
     1981 cctgcaagca ttgaattcag ggaaagttct gaagattcat ttttgtctgc cattataaat
     2041 tatactaata gctctacagt ccactttaag ttgtccccta catatgtatt atatatggca
     2101 tgccggtatg tattgtccaa ccagtacaga cctgacatca gccctacaga gcgcacacat
     2161 aaagtcattg cagtcgtcaa caagatggtg agcatgatgg agggtgtcat ccaggaagta
     2221 gaccaggttg accagaaaca gaagaatatt gcaggggcac ttgccttctg gatggcaaat
     2281 gcatctgaac ttctcaactt cattaagcaa gaccgagacc ttagtcggat cacactggat
     2341 gctcaagatg ttttagcaca tttggttcaa atggcattta aatacttggt tcactgtctt
     2401 caatcagaac ttaataatta catgccagcc tttctagatg accctgaaga gaacagtctg
     2461 caacgaccaa aaatagatga tgtgctgcac acgctcacag gagccatgtc cttgctacga
     2521 cgctgcagag tcaatgccgc cctgaccatc cagctcttct ctcagctctt ccacttcatc
     2581 aatatgtggc tgttcaatag attggtgacc gacccagatt cggggctgtg ctcccattac
     2641 tggggtgcga ttatccgtca gcagttgggc catattgaag cctgggctga gaagcagggg
     2701 ctggaactgg ctgcggactg tcatctgagc aggatcgtgc aggcaacgac tttgcttacc
     2761 atggataagt atgcacctga tgacattcca aatataaaca gcacctgctt taagttaaat
     2821 tcattacaac ttcaagcctt attacagaac tatcactgtg cacctgatga gccttttatc
     2881 ccaacggatc ttatagaaaa tgtagtgact gtggctgaaa acactgccga tgagctggcc
     2941 cgcagtgatg gaagggaagt gcagttggag gaggatcctg atctgcagct gccgtttctt
     3001 ttgccagaag atggttattc ttgtgatgtt gtcagaaaca ttccaaatgg tttacaagaa
     3061 tttttagacc ctctgtgcca gagaggattt tgcaggttaa ttcctcacac acgttcacca
     3121 ggtacttgga caatatattt tgaaggtgca gattatgaaa gtcaccttct gcgtgagaac
     3181 acagagctgg ctcagcctct gaggaaagaa cctgaaataa tcactgtgac cctaaaaaag
     3241 cagaatggaa tgggccttag cattgttgca gcaaagggtg ctggtcaaga taaactagga
     3301 atctatgtga agtcggttgt gaaaggaggt gctgcagatg tggatggacg tctagctgca
     3361 ggtgatcagc tcctcagtgt ggatggacga agtctggttg gactctctca ggaaagggcg
     3421 gcagaactca tgacaagaac aagctctgtg gtgacactgg aagtagcaaa gcagggtgcc
     3481 atctaccacg gtctggccac ccttctcaat cagccatccc ccatgatgca gagaatttca
     3541 gatcgtcgtg gctcaggtaa accccgacca aagagtgaag gctttgagct ctataataat
     3601 tcaactcaaa atgggtctcc tgagagtcct cagctgcctt gggcagaata tagtgaacca
     3661 aagaaattgc ctggtgatga cagactgatg aaaaatagag ctgatcaccg ttccagcccc
     3721 aacgtagcaa atcagcctcc tagtcctgga gggaaaagtg catatgcctc tggaacaaca
     3781 gcgaagataa catctgtctc tactggaaac ctctgcactg aggagcagac gcctccgcct
     3841 agacctgaag cctaccccat ccccactcag acgtacacca gagagtattt taccttccca
     3901 gcttccaaat cccaggatcg gatggctcct cctcagaacc agtggccaaa ttatgaggaa
     3961 aagccacata tgcacacaga tagtaatcat tccagtattg caattcagcg tgttacacgt
     4021 tcccaagaag aacttcgaga agataaagct taccaacttg agcggcatcg aatagaggca
     4081 gctatggacc gaaagtctga tagtgatatg tggataaatc agagctcctc actggactcc
     4141 agtacctcta gccaggagca tctgaaccat tcctctaagt cggtcacccc tgcttccaca
     4201 ctgaccaaaa gtggccctgg ccgttggaaa acaccagcag ccataccggc cacccctgtg
     4261 gccgtctccc agccaatccg aacagacctg cctccgccac ccccgccacc tccagtccac
     4321 tatgccggtg atttcgatgg aatgtccatg gatttgcctc tcccaccacc cccttccgcc
     4381 aaccagatag ggctgccgtc tgcgcaggtg gctgctgctg aacggagaaa gagagaagaa
     4441 catcagcgtt ggtatgagaa ggagaaggcc cgcctggagg aggagcggga gaggaagcgg
     4501 agagagcagg agaggaagtt gggccagatg cgcactcagt ccttaaaccc tgctccgttt
     4561 tctcccctga ctgcacagca gatgaagccc gaaaagcctt ccacactcca gcggccacag
     4621 gaaacagtca ttcgggagct gcagcctcag cagcagcccc gcacgatcga gcgcagagac
     4681 ttgcagtaca ttacagtcag caaagaggag ctttcctcgg gggacagtct gtcccccgac
     4741 ccgtggaagc gggacgccaa ggagaagctg gagaagcagc agcagatgca catcgtggac
     4801 atgctgagca aggagatcca ggagctccag agcaaaccgg accgcagcgc cgaggagagc
     4861 gaccggctgc gcaagctcat gctggagtgg cagttccaga agagactcca ggagtcgaag
     4921 cagaaggacg aagatgacga ggaggaggag gacgatgatg tggacaccat gctgatcatg
     4981 cagcgcctgg aggctgaacg aagagcgagg ttgcaggacg aggagcggag gcggcagcag
     5041 cagttagaag agatgcgcaa gcgggaagcg gaagaccgag cgaggcaaga ggaagagcgc
     5101 cggcggcagg aggaggagcg aacaaaacga gacgctgaag aaaagaggcg acaggaagaa
     5161 gggtattaca gccgcctgga agccgagagg cgcagacagc acgacgaggc ggcgcgcagg
     5221 ttgctggagc ccgaggcgcc cggtctgtgc cgccctccgc ttccccggga ctacgagccc
     5281 ccgtccccgt cccccgcgcc cggcgcccct cctcccccgc ctcagcgaaa cgcctcctac
     5341 ctcaaaacac aggtcctctc ccccgactcg ctgttcactg ccaagtttgt tgcatacaat
     5401 gaggaggagg aggaggagga ctgcagccta gcaggaccaa actcttaccc aggatctact
     5461 ggagcagctg ttggagccca tgacgcctgt cgggatgcaa aagagaagcg ctctaaaagc
     5521 caagatgcag attcacctgg aagttctggg gcccctgaaa acttgacatt caaggaacgc
     5581 cagcgtcttt tttcacaagg tcaagatgta tccaataaag tgaaagcttc tcgtaaatta
     5641 acagaactgg agaatgaact gaacacaaag tgaaagaaaa tgaggagaac actttgtatt
     5701 taccccaaaa tcttttcagt tgtgggtttg taggtgcgag tttgaagagg aaaagaggaa
     5761 gggggttata tttctaagtg atttacaatt tctgtttcta aaattgttgg gatttagaaa
     5821 tgtttcaatt ccaagaaatt ttattttact ttaccatgag attgccattt atgtaattta
     5881 aacactgtct agtttcttaa ttatctaact cacacgaggg taagtttttt gttggtttct
     5941 tttttggagt cctgagttca aaggggacag aactaagggc aggagaagag aatggatgtg
     6001 tcttctctcc catcttcccc tcatcatcga ccgaggagca caaagaagtt ctgtttctgt
     6061 ttttttcaga aactttattt tagctgtcaa gcagagaata gcttgaacta ttcagactat
     6121 tgttggcact gtttagtagt gaccacacgc tcttctaccc gggaaggaac atatgacgac
     6181 atgctcctcc catgtcccct gatggtgtct gttcccacaa tatccttcta gtctgcatcg
     6241 tgaagtggct taggccaaag cttcctgtgt gatgacgcac gtccgagact gttccatctc
     6301 attcctcgca aatttgagct cttctgatca attctaaata tttgattaat tttaattctg
     6361 aaagaaaatt ttgaggattt aagcttaggg gattttattt ttataaatac cagattattg
     6421 ttagacttaa taaatcactg ttaaatttaa agatgacagt taccttggaa agttcactaa
     6481 tacttcgctc caaggcgtct gtaaaagaag atatctttat tggagcaatg ttcatgtgac
     6541 tgggaatgac agaagaatgg gagatgagta gggacccctc aagcacagct gtcactcaga
     6601 aattttaaat ttgaaaaaga aatcgatttt catctgtatg ccgtcaagga aggaattcag
     6661 ttacagggca tctgtaactt aaatattgta agaataactc atatggaagt tcaagctatt
     6721 tttatactat aatagagtta tttaatttta atttgttgaa ttattagtta ccactgtcat
     6781 ttcttcagct atggatatgt ggctgatgtt ggggagacgg acctcagtgt gttttatatt
     6841 gtctggtgtt aagatgataa attactttgt gattacaagc aaattttcta tttgacaaag
     6901 cccttggtgg agtccaaatg atttttctgt accatattgt tctcttacac atacgtatgt
     6961 taggaaaata ggcacacttt caaagagaat gttctcttac acatacgtat gttaggaaaa
     7021 tagacacatt ttcaaagaga atgttctctt acatatgtta ggaaaatagg cacactttca
     7081 aagagaggta taggggcttt ttactgcctt cgaaatcact tttaataaaa gttgtatgat
     7141 aaatcattaa aaatgcgagt gtaaatattt ttttttacct ctatcagggt ttttatttga
     7201 aagtgtgctc ttaccatttc cttgtttgag taagagagtg aatggttctg agtgtttagg
     7261 aaggatttgc ttgcttgcag cagaagttct aggaaaagca ggcacctccc aaatatcttg
     7321 aggacactgg cagccttatg gtgactaaat ccacactttc tttaaagaga tgcagatgaa
     7381 ttacttttct gttaatatat agaaaaatac acaggagaac atgcagatac atgtaaatat
     7441 ctacatacag ggatatttgt atgtgtggtt gtgaaaccca tacacaagtg gatttggcca
     7501 tctttgggca gtgaggtcaa atgtgaatat gtgtaaaagt gcttttcaga actgcattac
     7561 tatgagaaaa catcaaaact gctgtagttt tccatttcta ctgtatttca gttgcaacct
     7621 atttttaata aactttgtat gtatttaagt gtatttgcta ttgttttgaa agtgctgaca
     7681 aagtttatat ttgtaacatc agccttcctc gcccatctct ttccatctgt ctcttcttca
     7741 ttaaacactt actgagtatg a
//
