LOCUS       NM_001386888            7806 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens afadin, adherens junction formation factor (AFDN),
            transcript variant 8, mRNA.
ACCESSION   NM_001386888 XM_017010879
VERSION     NM_001386888.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Awotoye,W., Mossey,P.A., Hetmanski,J.B., Gowans,L.J.J.,
            Eshete,M.A., Adeyemo,W.L., Alade,A., Zeng,E., Adamson,O., James,O.,
            Fashina,A., Ogunlewe,M.O., Naicker,T., Adeleke,C., Busch,T., Li,M.,
            Petrin,A., Oladayo,A., Kayali,S., Olotu,J., Sule,V., Hassan,M.,
            Pape,J., Aladenika,E.T., Donkor,P., Arthur,F.K.N., Obiri-Yeboah,S.,
            Sabbah,D.K., Agbenorku,P., Ray,D., Plange-Rhule,G., Oti,A.A.,
            Albokhari,D., Sobreira,N., Dunnwald,M., Beaty,T.H., Taub,M.,
            Marazita,M.L., Adeyemo,A.A., Murray,J.C. and Butali,A.
  TITLE     Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft
            Lip With or Without Cleft Palate
  JOURNAL   Cleft Palate Craniofac J 61 (4), 697-705 (2024)
   PUBMED   36384317
  REMARK    GeneRIF: Damaging Mutations in AFDN Contribute to Risk of
            Nonsyndromic Cleft Lip With or Without Cleft Palate.
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Zhang,M.X., Shi,W.Z., Liu,J.X., Wang,C.J., Li,Y., Wang,W. and
            Jiang,B.
  TITLE     [Clinical characteristics and prognosis of MLL-AF6 positive
            patients with acute myeloid leukemia]
  JOURNAL   Beijing Da Xue Xue Bao Yi Xue Ban 53 (5), 915-920 (2021)
   PUBMED   34650294
  REMARK    GeneRIF: [Clinical characteristics and prognosis of MLL-AF6
            positive patients with acute myeloid leukemia].
REFERENCE   3  (bases 1 to 7806)
  AUTHORS   Baskaran,Y., Tay,F.P., Ng,E.Y.W., Swa,C.L.F., Wee,S., Gunaratne,J.
            and Manser,E.
  TITLE     Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions
  JOURNAL   Nat Commun 12 (1), 5315 (2021)
   PUBMED   34493720
  REMARK    GeneRIF: Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7806)
  AUTHORS   Lorger,M. and Moelling,K.
  TITLE     Regulation of epithelial wound closure and intercellular adhesion
            by interaction of AF6 with actin cytoskeleton
  JOURNAL   J Cell Sci 119 (Pt 16), 3385-3398 (2006)
   PUBMED   16882694
REFERENCE   5  (bases 1 to 7806)
  AUTHORS   Saito,S., Matsushima,M., Shirahama,S., Minaguchi,T., Kanamori,Y.,
            Minami,M. and Nakamura,Y.
  TITLE     Complete genomic structure DNA polymorphisms, and alternative
            splicing of the human AF-6 gene
  JOURNAL   DNA Res 5 (2), 115-120 (1998)
   PUBMED   9679199
REFERENCE   6  (bases 1 to 7806)
  AUTHORS   Mandai,K., Nakanishi,H., Satoh,A., Obaishi,H., Wada,M.,
            Nishioka,H., Itoh,M., Mizoguchi,A., Aoki,T., Fujimoto,T.,
            Matsuda,Y., Tsukita,S. and Takai,Y.
  TITLE     Afadin: A novel actin filament-binding protein with one PDZ domain
            localized at cadherin-based cell-to-cell adherens junction
  JOURNAL   J Cell Biol 139 (2), 517-528 (1997)
   PUBMED   9334353
  REMARK    Erratum:[J Cell Biol 1997 Nov 17;139(4):1060]
REFERENCE   7  (bases 1 to 7806)
  AUTHORS   Joh,T., Yamamoto,K., Kagami,Y., Kakuda,H., Sato,T., Yamamoto,T.,
            Takahashi,T., Ueda,R., Kaibuchi,K. and Seto,M.
  TITLE     Chimeric MLL products with a Ras binding cytoplasmic protein AF6
            involved in t(6;11) (q27;q23) leukemia localize in the nucleus
  JOURNAL   Oncogene 15 (14), 1681-1687 (1997)
   PUBMED   9349501
REFERENCE   8  (bases 1 to 7806)
  AUTHORS   Tanabe,S., Zeleznik-Le,N.J., Kobayashi,H., Vignon,C., Espinosa,R.
            3rd, LeBeau,M.M., Thirman,M.J. and Rowley,J.D.
  TITLE     Analysis of the t(6;11)(q27;q23) in leukemia shows a consistent
            breakpoint in AF6 in three patients and in the ML-2 cell line
  JOURNAL   Genes Chromosomes Cancer 15 (4), 206-216 (1996)
   PUBMED   8703846
REFERENCE   9  (bases 1 to 7806)
  AUTHORS   Saha,V., Lillington,D.M., Shelling,A.N., Chaplin,T., Yaspo,M.L.,
            Ganesan,T.S. and Young,B.D.
  TITLE     AF6 gene on chromosome band 6q27 maps distal to the minimal region
            of deletion in epithelial ovarian cancer
  JOURNAL   Genes Chromosomes Cancer 14 (3), 220-222 (1995)
   PUBMED   8589040
REFERENCE   10 (bases 1 to 7806)
  AUTHORS   Prasad,R., Gu,Y., Alder,H., Nakamura,T., Canaani,O., Saito,H.,
            Huebner,K., Gale,R.P., Nowell,P.C., Kuriyama,K. et al.
  TITLE     Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in
            acute myeloid leukemias with the t(6;11) chromosome translocation
  JOURNAL   Cancer Res 53 (23), 5624-5628 (1993)
   PUBMED   8242616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL009178.4, AL731868.5 and
            AL049698.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 15, 2020 this sequence version replaced XM_017010879.1.
            
            Summary: This gene encodes a multi-domain protein involved in
            signaling and organization of cell junctions during embryogenesis.
            It has also been identified as the fusion partner of acute
            lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid
            leukemias with t(6;11)(q27;q23) translocation. Alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene, however, not all have been fully
            characterized.[provided by RefSeq, May 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1968189 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683244.1/ ENSP00000507324.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-327               AL009178.4         12383-12709         c
            328-523             AL731868.5         26940-27135
            524-636             AL731868.5         32775-32887
            637-800             AL731868.5         34603-34766
            801-961             AL731868.5         37724-37884
            962-1119            AL731868.5         42749-42906
            1120-1231           AL731868.5         51604-51715
            1232-1399           AL731868.5         53251-53418
            1400-1444           AL049698.3         84427-84471         c
            1445-1539           AL049698.3         81361-81455         c
            1540-1802           AL049698.3         79866-80128         c
            1803-1872           AL049698.3         75947-76016         c
            1873-1991           AL049698.3         71044-71162         c
            1992-2053           AL049698.3         67171-67232         c
            2054-2259           AL049698.3         66844-67049         c
            2260-2280           AL049698.3         64910-64930         c
            2281-2426           AL049698.3         64020-64165         c
            2427-2521           AL049698.3         63595-63689         c
            2522-2787           AL049698.3         62900-63165         c
            2788-2931           AL049698.3         61101-61244         c
            2932-3130           AL049698.3         59400-59598         c
            3131-3234           AL049698.3         55374-55477         c
            3235-3321           AL049698.3         53242-53328         c
            3322-3387           AL049698.3         35139-35204         c
            3388-3461           AL049698.3         34858-34931         c
            3462-3580           AL049698.3         34274-34392         c
            3581-3775           AL049698.3         31432-31626         c
            3776-3867           AL049698.3         30389-30480         c
            3868-4053           AL049698.3         29855-30040         c
            4054-5055           AL049698.3         26146-27147         c
            5056-5190           AL049698.3         15766-15900         c
            5191-5479           AL049698.3         12288-12576         c
            5480-5564           AL049698.3         9135-9219           c
            5565-7806           AL049698.3         6310-8551           c
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..7806
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="afadin, adherens junction formation factor"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
     exon            1..327
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..5718
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="isoform 8 is encoded by transcript variant 8;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 4; myeloid/lymphoid
                     or mixed-lineage leukemia; translocated to, 4; ALL1-fused
                     gene from chromosome 6 protein; protein AF-6"
                     /codon_start=1
                     /product="afadin isoform 8"
                     /protein_id="NP_001373817.1"
                     /db_xref="CCDS:CCDS94033.1"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
                     /translation="MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMR
                     FYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGER
                     RLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRT
                     LSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNP
                     EVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADF
                     AVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPS
                     DKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSP
                     GRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
                     DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAL
                     AKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASST
                     AERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTN
                     SSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVD
                     QVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHC
                     LQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLF
                     HFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQA
                     TTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAE
                     NTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFC
                     RLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIV
                     AAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRT
                     SSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNG
                     SPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKI
                     TSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEK
                     PHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLD
                     SSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPP
                     PVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEE
                     RERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQP
                     RTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQS
                     KPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRA
                     RLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLE
                     AERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQV
                     LSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDA
                     DSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK"
     exon            328..523
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            524..636
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            637..800
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            801..961
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1119
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1231
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1399
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1444
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1539
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1802
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1872
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1991
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2053
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2259
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2280
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2426
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2521
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2522..2787
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2931
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..3130
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3234
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3235..3321
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3387
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3388..3461
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3462..3580
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3581..3775
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3776..3867
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..4053
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4054..5055
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5056..5190
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5191..5479
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5480..5564
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5565..7806
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7785..7790
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="hexamer: ATTAAA"
     polyA_site      7806
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="major polyA site"
ORIGIN      
        1 gcacaccggc cggcggggct gaggcggcgc ggcgcggcgc ggactgagcc ccaggcggag
       61 gccaagtcgg aggacgcggc gcggccgcgg aggcggaggc agccgcggag gcggaggcgg
      121 ccggcggggg gtggcgaggg gcgccgggcc cccgcggacc tgtcgtcctc ggcccgtcct
      181 ccggccccgg ccccgcgcgg ctgaggaggc gcggccagga ccatgtcggc gggcggccgt
      241 gacgaggagc ggcggaagct ggccgacatc atccaccact ggaacgccaa ccggctggac
      301 ctgttcgaga tcagccagcc gaccgaggat ttggagttcc atggagtgat gagattttat
      361 tttcaagata aagctgctgg aaactttgca acaaaatgta ttcgggtctc tagtactgcc
      421 accactcaag atgtaatcga aacgctcgcg gagaaatttc gacctgatat gcgaatgctg
      481 tcctctccca agtattcact ctatgaagtg catgtcagcg gagaaagaag attggatata
      541 gatgagaaac ctctagttgt acaactgaat tggaacaaag atgatcggga aggcagattt
      601 gttcttaaga atgagaatga cgccattcct cctaagaagg ctcaaagtaa tggacctgaa
      661 aagcaggaaa aagaaggggt tatccagaac ttcaagagaa ctctctcaaa gaaagaaaag
      721 aaggaaaaaa agaagagaga aaaagaggca ttgcgacagg catctgataa agatgataga
      781 cctttccaag gggaggatgt tgaaaattct cgactggctg ctgaggttta caaagacatg
      841 ccggaaacca gctttactcg aaccatttct aatcctgagg tggttatgaa acgacggagg
      901 cagcaaaaat tggaaaagag aatgcaggaa tttcggagct cagatgggcg gcctgattca
      961 ggtggaacat tgagaattta tgcagatagt ttaaaaccaa atattcccta caagacaatc
     1021 ctgctgtcta ctacagatcc tgcagacttt gctgtggctg aagctttaga gaagtatggt
     1081 ctggaaaaag aaaaccctaa ggattactgc atcgcccggg ttatgcttcc tcctggagcc
     1141 cagcattctg atgaaaaggg tgctaaagaa attattcttg atgatgatga gtgtccttta
     1201 caaatcttca gggaatggcc aagtgacaaa gggattttag tctttcagtt gaagaggagg
     1261 ccaccagacc acatcccaaa gaaaaccaag aaacacttgg aaggcaagac acccaaggga
     1321 aaggagagag ctgacgggtc tggctatggc tccacccttc ctccggagaa gctgccctat
     1381 ttagtagagt taagcccagg gagaaggaat cactttgcct actacaacta tcacacttac
     1441 gaagatggtt ctgactctag agataagcca aagctttacc gccttcagtt aagtgttact
     1501 gaagttggga cagaaaagtt ggatgacaac tctatccagt tgtttggccc aggaattcag
     1561 ccccatcact gtgaccttac caacatggat ggagtggtca ctgtgacgcc cagaagtatg
     1621 gacgcagaaa cctacgtgga aggccagcgc atctcagaaa ccaccatgct gcagagtggc
     1681 atgaaagtgc agtttggggc gtcccatgta tttaagtttg tggaccccag tcaggatcat
     1741 gctcttgcaa aaagatctgt ggatggaggc ctgatggtta agggcccaag acataaacct
     1801 ggaattgttc aggagacaac ttttgatttg ggaggagata ttcatagtgg gacagcatta
     1861 ccgacaagca agagcaccac taggctggac agcgacagag tgtcgtctgc ctctagcaca
     1921 gccgagcggg gaatggtgaa gccgatgatc agagtagaac agcagccaga ttatcgcagg
     1981 caagaaagca gaacacagga tgcttctggg cctgagctga tactacctgc aagcattgaa
     2041 ttcagggaaa gttctgaaga ttcatttttg tctgccatta taaattatac taatagctct
     2101 acagtccact ttaagttgtc ccctacatat gtattatata tggcatgccg gtatgtattg
     2161 tccaaccagt acagacctga catcagccct acagagcgca cacataaagt cattgcagtc
     2221 gtcaacaaga tggtgagcat gatggagggt gtcatccagg aagtagacca ggttgaccag
     2281 aaacagaaga atattgcagg ggcacttgcc ttctggatgg caaatgcatc tgaacttctc
     2341 aacttcatta agcaagaccg agaccttagt cggatcacac tggatgctca agatgtttta
     2401 gcacatttgg ttcaaatggc atttaaatac ttggttcact gtcttcaatc agaacttaat
     2461 aattacatgc cagcctttct agatgaccct gaagagaaca gtctgcaacg accaaaaata
     2521 gatgatgtgc tgcacacgct cacaggagcc atgtccttgc tacgacgctg cagagtcaat
     2581 gccgccctga ccatccagct cttctctcag ctcttccact tcatcaatat gtggctgttc
     2641 aatagattgg tgaccgaccc agattcgggg ctgtgctccc attactgggg tgcgattatc
     2701 cgtcagcagt tgggccatat tgaagcctgg gctgagaagc aggggctgga actggctgcg
     2761 gactgtcatc tgagcaggat cgtgcaggca acgactttgc ttaccatgga taagtatgca
     2821 cctgatgaca ttccaaatat aaacagcacc tgctttaagt taaattcatt acaacttcaa
     2881 gccttattac agaactatca ctgtgcacct gatgagcctt ttatcccaac ggatcttata
     2941 gaaaatgtag tgactgtggc tgaaaacact gccgatgagc tggcccgcag tgatggaagg
     3001 gaagtgcagt tggaggagga tcctgatctg cagctgccgt ttcttttgcc agaagatggt
     3061 tattcttgtg atgttgtcag aaacattcca aatggtttac aagaattttt agaccctctg
     3121 tgccagagag gattttgcag gttaattcct cacacacgtt caccaggtac ttggacaata
     3181 tattttgaag gtgcagatta tgaaagtcac cttctgcgtg agaacacaga gctggctcag
     3241 cctctgagga aagaacctga aataatcact gtgaccctaa aaaagcagaa tggaatgggc
     3301 cttagcattg ttgcagcaaa gggtgctggt caagataaac taggaatcta tgtgaagtcg
     3361 gttgtgaaag gaggtgctgc agatgtggat ggacgtctag ctgcaggtga tcagctcctc
     3421 agtgtggatg gacgaagtct ggttggactc tctcaggaaa gggcggcaga actcatgaca
     3481 agaacaagct ctgtggtgac actggaagta gcaaagcagg gtgccatcta ccacggtctg
     3541 gccacccttc tcaatcagcc atcccccatg atgcagagaa tttcagatcg tcgtggctca
     3601 ggtaaacccc gaccaaagag tgaaggcttt gagctctata ataattcaac tcaaaatggg
     3661 tctcctgaga gtcctcagct gccttgggca gaatatagtg aaccaaagaa attgcctggt
     3721 gatgacagac tgatgaaaaa tagagctgat caccgttcca gccccaacgt agcaaatcag
     3781 cctcctagtc ctggagggaa aagtgcatat gcctctggaa caacagcgaa gataacatct
     3841 gtctctactg gaaacctctg cactgaggag cagacgcctc cgcctagacc tgaagcctac
     3901 cccatcccca ctcagacgta caccagagag tattttacct tcccagcttc caaatcccag
     3961 gatcggatgg ctcctcctca gaaccagtgg ccaaattatg aggaaaagcc acatatgcac
     4021 acagatagta atcattccag tattgcaatt cagcgtgtta cacgttccca agaagaactt
     4081 cgagaagata aagcttacca acttgagcgg catcgaatag aggcagctat ggaccgaaag
     4141 tctgatagtg atatgtggat aaatcagagc tcctcactgg actccagtac ctctagccag
     4201 gagcatctga accattcctc taagtcggtc acccctgctt ccacactgac caaaagtggc
     4261 cctggccgtt ggaaaacacc agcagccata ccggccaccc ctgtggccgt ctcccagcca
     4321 atccgaacag acctgcctcc gccacccccg ccacctccag tccactatgc cggtgatttc
     4381 gatggaatgt ccatggattt gcctctccca ccaccccctt ccgccaacca gatagggctg
     4441 ccgtctgcgc aggtggctgc tgctgaacgg agaaagagag aagaacatca gcgttggtat
     4501 gagaaggaga aggcccgcct ggaggaggag cgggagagga agcggagaga gcaggagagg
     4561 aagttgggcc agatgcgcac tcagtcctta aaccctgctc cgttttctcc cctgactgca
     4621 cagcagatga agcccgaaaa gccttccaca ctccagcggc cacaggaaac agtcattcgg
     4681 gagctgcagc ctcagcagca gccccgcacg atcgagcgca gagacttgca gtacattaca
     4741 gtcagcaaag aggagctttc ctcgggggac agtctgtccc ccgacccgtg gaagcgggac
     4801 gccaaggaga agctggagaa gcagcagcag atgcacatcg tggacatgct gagcaaggag
     4861 atccaggagc tccagagcaa accggaccgc agcgccgagg agagcgaccg gctgcgcaag
     4921 ctcatgctgg agtggcagtt ccagaagaga ctccaggagt cgaagcagaa ggacgaagat
     4981 gacgaggagg aggaggacga tgatgtggac accatgctga tcatgcagcg cctggaggct
     5041 gaacgaagag cgaggttgca ggacgaggag cggaggcggc agcagcagtt agaagagatg
     5101 cgcaagcggg aagcggaaga ccgagcgagg caagaggaag agcgccggcg gcaggaggag
     5161 gagcgaacaa aacgagacgc tgaagaaaag aggcgacagg aagaagggta ttacagccgc
     5221 ctggaagccg agaggcgcag acagcacgac gaggcggcgc gcaggttgct ggagcccgag
     5281 gcgcccggtc tgtgccgccc tccgcttccc cgggactacg agcccccgtc cccgtccccc
     5341 gcgcccggcg cccctcctcc cccgcctcag cgaaacgcct cctacctcaa aacacaggtc
     5401 ctctcccccg actcgctgtt cactgccaag tttgttgcat acaatgagga ggaggaggag
     5461 gaggactgca gcctagcagg accaaactct tacccaggat ctactggagc agctgttgga
     5521 gcccatgacg cctgtcggga tgcaaaagag aagcgctcta aaagccaaga tgcagattca
     5581 cctggaagtt ctggggcccc tgaaaacttg acattcaagg aacgccagcg tcttttttca
     5641 caaggtcaag atgtatccaa taaagtgaaa gcttctcgta aattaacaga actggagaat
     5701 gaactgaaca caaagtgaaa gaaaatgagg agaacacttt gtatttaccc caaaatcttt
     5761 tcagttgtgg gtttgtaggt gcgagtttga agaggaaaag aggaaggggg ttatatttct
     5821 aagtgattta caatttctgt ttctaaaatt gttgggattt agaaatgttt caattccaag
     5881 aaattttatt ttactttacc atgagattgc catttatgta atttaaacac tgtctagttt
     5941 cttaattatc taactcacac gagggtaagt tttttgttgg tttctttttt ggagtcctga
     6001 gttcaaaggg gacagaacta agggcaggag aagagaatgg atgtgtcttc tctcccatct
     6061 tcccctcatc atcgaccgag gagcacaaag aagttctgtt tctgtttttt tcagaaactt
     6121 tattttagct gtcaagcaga gaatagcttg aactattcag actattgttg gcactgttta
     6181 gtagtgacca cacgctcttc tacccgggaa ggaacatatg acgacatgct cctcccatgt
     6241 cccctgatgg tgtctgttcc cacaatatcc ttctagtctg catcgtgaag tggcttaggc
     6301 caaagcttcc tgtgtgatga cgcacgtccg agactgttcc atctcattcc tcgcaaattt
     6361 gagctcttct gatcaattct aaatatttga ttaattttaa ttctgaaaga aaattttgag
     6421 gatttaagct taggggattt tatttttata aataccagat tattgttaga cttaataaat
     6481 cactgttaaa tttaaagatg acagttacct tggaaagttc actaatactt cgctccaagg
     6541 cgtctgtaaa agaagatatc tttattggag caatgttcat gtgactggga atgacagaag
     6601 aatgggagat gagtagggac ccctcaagca cagctgtcac tcagaaattt taaatttgaa
     6661 aaagaaatcg attttcatct gtatgccgtc aaggaaggaa ttcagttaca gggcatctgt
     6721 aacttaaata ttgtaagaat aactcatatg gaagttcaag ctatttttat actataatag
     6781 agttatttaa ttttaatttg ttgaattatt agttaccact gtcatttctt cagctatgga
     6841 tatgtggctg atgttgggga gacggacctc agtgtgtttt atattgtctg gtgttaagat
     6901 gataaattac tttgtgatta caagcaaatt ttctatttga caaagccctt ggtggagtcc
     6961 aaatgatttt tctgtaccat attgttctct tacacatacg tatgttagga aaataggcac
     7021 actttcaaag agaatgttct cttacacata cgtatgttag gaaaatagac acattttcaa
     7081 agagaatgtt ctcttacata tgttaggaaa ataggcacac tttcaaagag aggtataggg
     7141 gctttttact gccttcgaaa tcacttttaa taaaagttgt atgataaatc attaaaaatg
     7201 cgagtgtaaa tatttttttt tacctctatc agggttttta tttgaaagtg tgctcttacc
     7261 atttccttgt ttgagtaaga gagtgaatgg ttctgagtgt ttaggaagga tttgcttgct
     7321 tgcagcagaa gttctaggaa aagcaggcac ctcccaaata tcttgaggac actggcagcc
     7381 ttatggtgac taaatccaca ctttctttaa agagatgcag atgaattact tttctgttaa
     7441 tatatagaaa aatacacagg agaacatgca gatacatgta aatatctaca tacagggata
     7501 tttgtatgtg tggttgtgaa acccatacac aagtggattt ggccatcttt gggcagtgag
     7561 gtcaaatgtg aatatgtgta aaagtgcttt tcagaactgc attactatga gaaaacatca
     7621 aaactgctgt agttttccat ttctactgta tttcagttgc aacctatttt taataaactt
     7681 tgtatgtatt taagtgtatt tgctattgtt ttgaaagtgc tgacaaagtt tatatttgta
     7741 acatcagcct tcctcgccca tctctttcca tctgtctctt cttcattaaa cacttactga
     7801 gtatga
//
