LOCUS       NM_001291964            7753 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens afadin, adherens junction formation factor (AFDN),
            transcript variant 4, mRNA.
ACCESSION   NM_001291964
VERSION     NM_001291964.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Awotoye,W., Mossey,P.A., Hetmanski,J.B., Gowans,L.J.J.,
            Eshete,M.A., Adeyemo,W.L., Alade,A., Zeng,E., Adamson,O., James,O.,
            Fashina,A., Ogunlewe,M.O., Naicker,T., Adeleke,C., Busch,T., Li,M.,
            Petrin,A., Oladayo,A., Kayali,S., Olotu,J., Sule,V., Hassan,M.,
            Pape,J., Aladenika,E.T., Donkor,P., Arthur,F.K.N., Obiri-Yeboah,S.,
            Sabbah,D.K., Agbenorku,P., Ray,D., Plange-Rhule,G., Oti,A.A.,
            Albokhari,D., Sobreira,N., Dunnwald,M., Beaty,T.H., Taub,M.,
            Marazita,M.L., Adeyemo,A.A., Murray,J.C. and Butali,A.
  TITLE     Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft
            Lip With or Without Cleft Palate
  JOURNAL   Cleft Palate Craniofac J 61 (4), 697-705 (2024)
   PUBMED   36384317
  REMARK    GeneRIF: Damaging Mutations in AFDN Contribute to Risk of
            Nonsyndromic Cleft Lip With or Without Cleft Palate.
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Zhang,M.X., Shi,W.Z., Liu,J.X., Wang,C.J., Li,Y., Wang,W. and
            Jiang,B.
  TITLE     [Clinical characteristics and prognosis of MLL-AF6 positive
            patients with acute myeloid leukemia]
  JOURNAL   Beijing Da Xue Xue Bao Yi Xue Ban 53 (5), 915-920 (2021)
   PUBMED   34650294
  REMARK    GeneRIF: [Clinical characteristics and prognosis of MLL-AF6
            positive patients with acute myeloid leukemia].
REFERENCE   3  (bases 1 to 7753)
  AUTHORS   Baskaran,Y., Tay,F.P., Ng,E.Y.W., Swa,C.L.F., Wee,S., Gunaratne,J.
            and Manser,E.
  TITLE     Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions
  JOURNAL   Nat Commun 12 (1), 5315 (2021)
   PUBMED   34493720
  REMARK    GeneRIF: Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7753)
  AUTHORS   Lorger,M. and Moelling,K.
  TITLE     Regulation of epithelial wound closure and intercellular adhesion
            by interaction of AF6 with actin cytoskeleton
  JOURNAL   J Cell Sci 119 (Pt 16), 3385-3398 (2006)
   PUBMED   16882694
REFERENCE   5  (bases 1 to 7753)
  AUTHORS   Saito,S., Matsushima,M., Shirahama,S., Minaguchi,T., Kanamori,Y.,
            Minami,M. and Nakamura,Y.
  TITLE     Complete genomic structure DNA polymorphisms, and alternative
            splicing of the human AF-6 gene
  JOURNAL   DNA Res 5 (2), 115-120 (1998)
   PUBMED   9679199
REFERENCE   6  (bases 1 to 7753)
  AUTHORS   Mandai,K., Nakanishi,H., Satoh,A., Obaishi,H., Wada,M.,
            Nishioka,H., Itoh,M., Mizoguchi,A., Aoki,T., Fujimoto,T.,
            Matsuda,Y., Tsukita,S. and Takai,Y.
  TITLE     Afadin: A novel actin filament-binding protein with one PDZ domain
            localized at cadherin-based cell-to-cell adherens junction
  JOURNAL   J Cell Biol 139 (2), 517-528 (1997)
   PUBMED   9334353
  REMARK    Erratum:[J Cell Biol 1997 Nov 17;139(4):1060]
REFERENCE   7  (bases 1 to 7753)
  AUTHORS   Joh,T., Yamamoto,K., Kagami,Y., Kakuda,H., Sato,T., Yamamoto,T.,
            Takahashi,T., Ueda,R., Kaibuchi,K. and Seto,M.
  TITLE     Chimeric MLL products with a Ras binding cytoplasmic protein AF6
            involved in t(6;11) (q27;q23) leukemia localize in the nucleus
  JOURNAL   Oncogene 15 (14), 1681-1687 (1997)
   PUBMED   9349501
REFERENCE   8  (bases 1 to 7753)
  AUTHORS   Tanabe,S., Zeleznik-Le,N.J., Kobayashi,H., Vignon,C., Espinosa,R.
            3rd, LeBeau,M.M., Thirman,M.J. and Rowley,J.D.
  TITLE     Analysis of the t(6;11)(q27;q23) in leukemia shows a consistent
            breakpoint in AF6 in three patients and in the ML-2 cell line
  JOURNAL   Genes Chromosomes Cancer 15 (4), 206-216 (1996)
   PUBMED   8703846
REFERENCE   9  (bases 1 to 7753)
  AUTHORS   Saha,V., Lillington,D.M., Shelling,A.N., Chaplin,T., Yaspo,M.L.,
            Ganesan,T.S. and Young,B.D.
  TITLE     AF6 gene on chromosome band 6q27 maps distal to the minimal region
            of deletion in epithelial ovarian cancer
  JOURNAL   Genes Chromosomes Cancer 14 (3), 220-222 (1995)
   PUBMED   8589040
REFERENCE   10 (bases 1 to 7753)
  AUTHORS   Prasad,R., Gu,Y., Alder,H., Nakamura,T., Canaani,O., Saito,H.,
            Huebner,K., Gale,R.P., Nowell,P.C., Kuriyama,K. et al.
  TITLE     Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in
            acute myeloid leukemias with the t(6;11) chromosome translocation
  JOURNAL   Cancer Res 53 (23), 5624-5628 (1993)
   PUBMED   8242616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB621809.1, AL009178.4,
            AL049698.3 and AL161973.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001291964.1.
            
            Summary: This gene encodes a multi-domain protein involved in
            signaling and organization of cell junctions during embryogenesis.
            It has also been identified as the fusion partner of acute
            lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid
            leukemias with t(6;11)(q27;q23) translocation. Alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene, however, not all have been fully
            characterized.[provided by RefSeq, May 2011].
            
            Transcript Variant: This variant (4) represents the longest
            transcript and encodes the longest isoform (4).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB621809.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-177               AB621809.1         1-177
            178-178             AL009178.4         11808-11808         c
            179-214             AB621809.1         179-214
            215-215             AL009178.4         11771-11771         c
            216-2899            AB621809.1         216-2899
            2900-2900           AL049698.3         59583-59583         c
            2901-3303           AB621809.1         2901-3303
            3304-3304           AL049698.3         35175-35175         c
            3305-3582           AB621809.1         3305-3582
            3583-3583           AL049698.3         31577-31577         c
            3584-5962           AB621809.1         3584-5962
            5963-7192           AL161973.1         4802-6031
            7193-7193           AL049698.3         6870-6870           c
            7194-7750           AL161973.1         6033-6589
            7751-7753           AL049698.3         6310-6312           c
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..7753
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="afadin, adherens junction formation factor"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
     exon            1..298
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            299..494
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..5665
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="isoform 4 is encoded by transcript variant 4;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 4; myeloid/lymphoid
                     or mixed-lineage leukemia; translocated to, 4; ALL1-fused
                     gene from chromosome 6 protein; protein AF-6"
                     /codon_start=1
                     /product="afadin isoform 4"
                     /protein_id="NP_001278893.1"
                     /db_xref="CCDS:CCDS78199.1"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
                     /translation="MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRML
                     SSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSN
                     GPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAE
                     VYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKP
                     NIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEI
                     ILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGY
                     GSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKL
                     DDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQF
                     GASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTS
                     KSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEF
                     RESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIA
                     VVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLV
                     QMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAA
                     LTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAA
                     DCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTD
                     LIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEF
                     LDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLK
                     KQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQ
                     ERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGF
                     ELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKS
                     AYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPP
                     QNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSD
                     MWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIR
                     TDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWY
                     EKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETV
                     IRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDM
                     LSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLI
                     MQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQE
                     EGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRN
                     ASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKE
                     KRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK"
     exon            495..610
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            611..774
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            775..935
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1093
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1205
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1373
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1418
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1513
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1776
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1846
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1965
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2027
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2028..2233
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2234..2379
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2474
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2475..2740
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2884
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3083
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3084..3187
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3274
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3340
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3414
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3533
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3728
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3729..3820
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3821..4006
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4007..5008
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5009..5137
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5138..5426
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5427..5511
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5512..7753
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7732..7737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="hexamer: ATTAAA"
     polyA_site      7753
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="major polyA site"
ORIGIN      
        1 gtggctcgcg gcgccccttc gccgtggccg ggatggagcc tgtgacgcgc gctgtgcttc
       61 gggttcataa acttgcaggc tgccagctgg gaggggaaag tcagtatcac aaagttcggg
      121 aggtgcgggc tgcctgatgg cacaggcccg gagtcggacg cctccgagga aaggcgcgcc
      181 ttctccacct tcggggccgc gaggaagggc cctctgcggc ggcctctgcc cggagggagg
      241 cgccggcccc aggctggctc cctgagagga ggaggaaatc acgctcgctc ctggcctgga
      301 tttggagttc catggagtga tgagatttta ttttcaagat aaagctgctg gaaactttgc
      361 aacaaaatgt attcgggtct ctagtactgc caccactcaa gatgtaatcg aaacgctcgc
      421 ggagaaattt cgacctgata tgcgaatgct gtcctctccc aagtattcac tctatgaagt
      481 gcatgtcagc ggagaagaaa gaagattgga tatagatgag aaacctctag ttgtacaact
      541 gaattggaac aaagatgatc gggaaggcag atttgttctt aagaatgaga atgacgccat
      601 tcctcctaag aaggctcaaa gtaatggacc tgaaaagcag gaaaaagaag gggttatcca
      661 gaacttcaag agaactctct caaagaaaga aaagaaggaa aaaaagaaga gagaaaaaga
      721 ggcattgcga caggcatctg ataaagatga tagacctttc caaggggagg atgttgaaaa
      781 ttctcgactg gctgctgagg tttacaaaga catgccggaa accagcttta ctcgaaccat
      841 ttctaatcct gaggtggtta tgaaacgacg gaggcagcaa aaattggaaa agagaatgca
      901 ggaatttcgg agctcagatg ggcggcctga ttcaggtgga acattgagaa tttatgcaga
      961 tagtttaaaa ccaaatattc cctacaagac aatcctgctg tctactacag atcctgcaga
     1021 ctttgctgtg gctgaagctt tagagaagta tggtctggaa aaagaaaacc ctaaggatta
     1081 ctgcatcgcc cgggttatgc ttcctcctgg agcccagcat tctgatgaaa agggtgctaa
     1141 agaaattatt cttgatgatg atgagtgtcc tttacaaatc ttcagggaat ggccaagtga
     1201 caaagggatt ttagtctttc agttgaagag gaggccacca gaccacatcc caaagaaaac
     1261 caagaaacac ttggaaggca agacacccaa gggaaaggag agagctgacg ggtctggcta
     1321 tggctccacc cttcctccgg agaagctgcc ctatttagta gagttaagcc cagggagaag
     1381 gaatcacttt gcctactaca actatcacac ttacgaagat ggttctgact ctagagataa
     1441 gccaaagctt taccgccttc agttaagtgt tactgaagtt gggacagaaa agttggatga
     1501 caactctatc cagttgtttg gcccaggaat tcagccccat cactgtgacc ttaccaacat
     1561 ggatggagtg gtcactgtga cgcccagaag tatggacgca gaaacctacg tggaaggcca
     1621 gcgcatctca gaaaccacca tgctgcagag tggcatgaaa gtgcagtttg gggcgtccca
     1681 tgtatttaag tttgtggacc ccagtcagga tcatgctctt gcaaaaagat ctgtggatgg
     1741 aggcctgatg gttaagggcc caagacataa acctggaatt gttcaggaga caacttttga
     1801 tttgggagga gatattcata gtgggacagc attaccgaca agcaagagca ccactaggct
     1861 ggacagcgac agagtgtcgt ctgcctctag cacagccgag cggggaatgg tgaagccgat
     1921 gatcagagta gaacagcagc cagattatcg caggcaagaa agcagaacac aggatgcttc
     1981 tgggcctgag ctgatactac ctgcaagcat tgaattcagg gaaagttctg aagattcatt
     2041 tttgtctgcc attataaatt atactaatag ctctacagtc cactttaagt tgtcccctac
     2101 atatgtatta tatatggcat gccggtatgt attgtccaac cagtacagac ctgacatcag
     2161 ccctacagag cgcacacata aagtcattgc agtcgtcaac aagatggtga gcatgatgga
     2221 gggtgtcatc cagaaacaga agaatattgc aggggcactt gccttctgga tggcaaatgc
     2281 atctgaactt ctcaacttca ttaagcaaga ccgagacctt agtcggatca cactggatgc
     2341 tcaagatgtt ttagcacatt tggttcaaat ggcatttaaa tacttggttc actgtcttca
     2401 atcagaactt aataattaca tgccagcctt tctagatgac cctgaagaga acagtctgca
     2461 acgaccaaaa atagatgatg tgctgcacac gctcacagga gccatgtcct tgctacgacg
     2521 ctgcagagtc aatgccgccc tgaccatcca gctcttctct cagctcttcc acttcatcaa
     2581 tatgtggctg ttcaatagat tggtgaccga cccagattcg gggctgtgct cccattactg
     2641 gggtgcgatt atccgtcagc agttgggcca tattgaagcc tgggctgaga agcaggggct
     2701 ggaactggct gcggactgtc atctgagcag gatcgtgcag gcaacgactt tgcttaccat
     2761 ggataagtat gcacctgatg acattccaaa tataaacagc acctgcttta agttaaattc
     2821 attacaactt caagccttat tacagaacta tcactgtgca cctgatgagc cttttatccc
     2881 aacggatctt atagaaaatg tagtgactgt ggctgaaaac actgccgatg agctggcccg
     2941 cagtgatgga agggaagtgc agttggagga ggatcctgat ctgcagctgc cgtttctttt
     3001 gccagaagat ggttattctt gtgatgttgt cagaaacatt ccaaatggtt tacaagaatt
     3061 tttagaccct ctgtgccaga gaggattttg caggttaatt cctcacacac gttcaccagg
     3121 tacttggaca atatattttg aaggtgcaga ttatgaaagt caccttctgc gtgagaacac
     3181 agagctggct cagcctctga ggaaagaacc tgaaataatc actgtgaccc taaaaaagca
     3241 gaatggaatg ggccttagca ttgttgcagc aaagggtgct ggtcaagata aactaggaat
     3301 ctatgtgaag tcggttgtga aaggaggtgc tgcagatgtg gatggacgtc tagctgcagg
     3361 tgatcagctc ctcagtgtgg atggacgaag tctggttgga ctctctcagg aaagggcggc
     3421 agaactcatg acaagaacaa gctctgtggt gacactggaa gtagcaaagc agggtgccat
     3481 ctaccacggt ctggccaccc ttctcaatca gccatccccc atgatgcaga gaatttcaga
     3541 tcgtcgtggc tcaggtaaac cccgaccaaa gagtgaaggc tttgagctct ataataattc
     3601 aactcaaaat gggtctcctg agagtcctca gctgccttgg gcagaatata gtgaaccaaa
     3661 gaaattgcct ggtgatgaca gactgatgaa aaatagagct gatcaccgtt ccagccccaa
     3721 cgtagcaaat cagcctccta gtcctggagg gaaaagtgca tatgcctctg gaacaacagc
     3781 gaagataaca tctgtctcta ctggaaacct ctgcactgag gagcagacgc ctccgcctag
     3841 acctgaagcc taccccatcc ccactcagac gtacaccaga gagtatttta ccttcccagc
     3901 ttccaaatcc caggatcgga tggctcctcc tcagaaccag tggccaaatt atgaggaaaa
     3961 gccacatatg cacacagata gtaatcattc cagtattgca attcagcgtg ttacacgttc
     4021 ccaagaagaa cttcgagaag ataaagctta ccaacttgag cggcatcgaa tagaggcagc
     4081 tatggaccga aagtctgata gtgatatgtg gataaatcag agctcctcac tggactccag
     4141 tacctctagc caggagcatc tgaaccattc ctctaagtcg gtcacccctg cttccacact
     4201 gaccaaaagt ggccctggcc gttggaaaac accagcagcc ataccggcca cccctgtggc
     4261 cgtctcccag ccaatccgaa cagacctgcc tccgccaccc ccgccacctc cagtccacta
     4321 tgccggtgat ttcgatggaa tgtccatgga tttgcctctc ccaccacccc cttccgccaa
     4381 ccagataggg ctgccgtctg cgcaggtggc tgctgctgaa cggagaaaga gagaagaaca
     4441 tcagcgttgg tatgagaagg agaaggcccg cctggaggag gagcgggaga ggaagcggag
     4501 agagcaggag aggaagttgg gccagatgcg cactcagtcc ttaaaccctg ctccgttttc
     4561 tcccctgact gcacagcaga tgaagcccga aaagccttcc acactccagc ggccacagga
     4621 aacagtcatt cgggagctgc agcctcagca gcagccccgc acgatcgagc gcagagactt
     4681 gcagtacatt acagtcagca aagaggagct ttcctcgggg gacagtctgt cccccgaccc
     4741 gtggaagcgg gacgccaagg agaagctgga gaagcagcag cagatgcaca tcgtggacat
     4801 gctgagcaag gagatccagg agctccagag caaaccggac cgcagcgccg aggagagcga
     4861 ccggctgcgc aagctcatgc tggagtggca gttccagaag agactccagg agtcgaagca
     4921 gaaggacgaa gatgacgagg aggaggagga cgatgatgtg gacaccatgc tgatcatgca
     4981 gcgcctggag gctgaacgaa gagcgaggga cgaggagcgg aggcggcagc agcagttaga
     5041 agagatgcgc aagcgggaag cggaagaccg agcgaggcaa gaggaagagc gccggcggca
     5101 ggaggaggag cgaacaaaac gagacgctga agaaaagagg cgacaggaag aagggtatta
     5161 cagccgcctg gaagccgaga ggcgcagaca gcacgacgag gcggcgcgca ggttgctgga
     5221 gcccgaggcg cccggtctgt gccgccctcc gcttccccgg gactacgagc ccccgtcccc
     5281 gtcccccgcg cccggcgccc ctcctccccc gcctcagcga aacgcctcct acctcaaaac
     5341 acaggtcctc tcccccgact cgctgttcac tgccaagttt gttgcataca atgaggagga
     5401 ggaggaggag gactgcagcc tagcaggacc aaactcttac ccaggatcta ctggagcagc
     5461 tgttggagcc catgacgcct gtcgggatgc aaaagagaag cgctctaaaa gccaagatgc
     5521 agattcacct ggaagttctg gggcccctga aaacttgaca ttcaaggaac gccagcgtct
     5581 tttttcacaa ggtcaagatg tatccaataa agtgaaagct tctcgtaaat taacagaact
     5641 ggagaatgaa ctgaacacaa agtgaaagaa aatgaggaga acactttgta tttaccccaa
     5701 aatcttttca gttgtgggtt tgtaggtgcg agtttgaaga ggaaaagagg aagggggtta
     5761 tatttctaag tgatttacaa tttctgtttc taaaattgtt gggatttaga aatgtttcaa
     5821 ttccaagaaa ttttatttta ctttaccatg agattgccat ttatgtaatt taaacactgt
     5881 ctagtttctt aattatctaa ctcacacgag ggtaagtttt ttgttggttt cttttttgga
     5941 gtcctgagtt caaaggggac agaactaagg gcaggagaag agaatggatg tgtcttctct
     6001 cccatcttcc cctcatcatc gaccgaggag cacaaagaag ttctgtttct gtttttttca
     6061 gaaactttat tttagctgtc aagcagagaa tagcttgaac tattcagact attgttggca
     6121 ctgtttagta gtgaccacac gctcttctac ccgggaagga acatatgacg acatgctcct
     6181 cccatgtccc ctgatggtgt ctgttcccac aatatccttc tagtctgcat cgtgaagtgg
     6241 cttaggccaa agcttcctgt gtgatgacgc acgtccgaga ctgttccatc tcattcctcg
     6301 caaatttgag ctcttctgat caattctaaa tatttgatta attttaattc tgaaagaaaa
     6361 ttttgaggat ttaagcttag gggattttat ttttataaat accagattat tgttagactt
     6421 aataaatcac tgttaaattt aaagatgaca gttaccttgg aaagttcact aatacttcgc
     6481 tccaaggcgt ctgtaaaaga agatatcttt attggagcaa tgttcatgtg actgggaatg
     6541 acagaagaat gggagatgag tagggacccc tcaagcacag ctgtcactca gaaattttaa
     6601 atttgaaaaa gaaatcgatt ttcatctgta tgccgtcaag gaaggaattc agttacaggg
     6661 catctgtaac ttaaatattg taagaataac tcatatggaa gttcaagcta tttttatact
     6721 ataatagagt tatttaattt taatttgttg aattattagt taccactgtc atttcttcag
     6781 ctatggatat gtggctgatg ttggggagac ggacctcagt gtgttttata ttgtctggtg
     6841 ttaagatgat aaattacttt gtgattacaa gcaaattttc tatttgacaa agcccttggt
     6901 ggagtccaaa tgatttttct gtaccatatt gttctcttac acatacgtat gttaggaaaa
     6961 taggcacact ttcaaagaga atgttctctt acacatacgt atgttaggaa aatagacaca
     7021 ttttcaaaga gaatgttctc ttacatatgt taggaaaata ggcacacttt caaagagagg
     7081 tataggggct ttttactgcc ttcgaaatca cttttaataa aagttgtatg ataaatcatt
     7141 aaaaatgcga gtgtaaatat ttttttttac ctctatcagg gtttttattt gaaagtgtgc
     7201 tcttaccatt tccttgtttg agtaagagag tgaatggttc tgagtgttta ggaaggattt
     7261 gcttgcttgc agcagaagtt ctaggaaaag caggcacctc ccaaatatct tgaggacact
     7321 ggcagcctta tggtgactaa atccacactt tctttaaaga gatgcagatg aattactttt
     7381 ctgttaatat atagaaaaat acacaggaga acatgcagat acatgtaaat atctacatac
     7441 agggatattt gtatgtgtgg ttgtgaaacc catacacaag tggatttggc catctttggg
     7501 cagtgaggtc aaatgtgaat atgtgtaaaa gtgcttttca gaactgcatt actatgagaa
     7561 aacatcaaaa ctgctgtagt tttccatttc tactgtattt cagttgcaac ctatttttaa
     7621 taaactttgt atgtatttaa gtgtatttgc tattgttttg aaagtgctga caaagtttat
     7681 atttgtaaca tcagccttcc tcgcccatct ctttccatct gtctcttctt cattaaacac
     7741 ttactgagta tga
//
