LOCUS       NM_001040000            7706 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens afadin, adherens junction formation factor (AFDN),
            transcript variant 2, mRNA.
ACCESSION   NM_001040000
VERSION     NM_001040000.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Awotoye,W., Mossey,P.A., Hetmanski,J.B., Gowans,L.J.J.,
            Eshete,M.A., Adeyemo,W.L., Alade,A., Zeng,E., Adamson,O., James,O.,
            Fashina,A., Ogunlewe,M.O., Naicker,T., Adeleke,C., Busch,T., Li,M.,
            Petrin,A., Oladayo,A., Kayali,S., Olotu,J., Sule,V., Hassan,M.,
            Pape,J., Aladenika,E.T., Donkor,P., Arthur,F.K.N., Obiri-Yeboah,S.,
            Sabbah,D.K., Agbenorku,P., Ray,D., Plange-Rhule,G., Oti,A.A.,
            Albokhari,D., Sobreira,N., Dunnwald,M., Beaty,T.H., Taub,M.,
            Marazita,M.L., Adeyemo,A.A., Murray,J.C. and Butali,A.
  TITLE     Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft
            Lip With or Without Cleft Palate
  JOURNAL   Cleft Palate Craniofac J 61 (4), 697-705 (2024)
   PUBMED   36384317
  REMARK    GeneRIF: Damaging Mutations in AFDN Contribute to Risk of
            Nonsyndromic Cleft Lip With or Without Cleft Palate.
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Zhang,M.X., Shi,W.Z., Liu,J.X., Wang,C.J., Li,Y., Wang,W. and
            Jiang,B.
  TITLE     [Clinical characteristics and prognosis of MLL-AF6 positive
            patients with acute myeloid leukemia]
  JOURNAL   Beijing Da Xue Xue Bao Yi Xue Ban 53 (5), 915-920 (2021)
   PUBMED   34650294
  REMARK    GeneRIF: [Clinical characteristics and prognosis of MLL-AF6
            positive patients with acute myeloid leukemia].
REFERENCE   3  (bases 1 to 7706)
  AUTHORS   Baskaran,Y., Tay,F.P., Ng,E.Y.W., Swa,C.L.F., Wee,S., Gunaratne,J.
            and Manser,E.
  TITLE     Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions
  JOURNAL   Nat Commun 12 (1), 5315 (2021)
   PUBMED   34493720
  REMARK    GeneRIF: Proximity proteomics identifies PAK4 as a component of
            Afadin-Nectin junctions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7706)
  AUTHORS   Lorger,M. and Moelling,K.
  TITLE     Regulation of epithelial wound closure and intercellular adhesion
            by interaction of AF6 with actin cytoskeleton
  JOURNAL   J Cell Sci 119 (Pt 16), 3385-3398 (2006)
   PUBMED   16882694
REFERENCE   5  (bases 1 to 7706)
  AUTHORS   Saito,S., Matsushima,M., Shirahama,S., Minaguchi,T., Kanamori,Y.,
            Minami,M. and Nakamura,Y.
  TITLE     Complete genomic structure DNA polymorphisms, and alternative
            splicing of the human AF-6 gene
  JOURNAL   DNA Res 5 (2), 115-120 (1998)
   PUBMED   9679199
REFERENCE   6  (bases 1 to 7706)
  AUTHORS   Mandai,K., Nakanishi,H., Satoh,A., Obaishi,H., Wada,M.,
            Nishioka,H., Itoh,M., Mizoguchi,A., Aoki,T., Fujimoto,T.,
            Matsuda,Y., Tsukita,S. and Takai,Y.
  TITLE     Afadin: A novel actin filament-binding protein with one PDZ domain
            localized at cadherin-based cell-to-cell adherens junction
  JOURNAL   J Cell Biol 139 (2), 517-528 (1997)
   PUBMED   9334353
  REMARK    Erratum:[J Cell Biol 1997 Nov 17;139(4):1060]
REFERENCE   7  (bases 1 to 7706)
  AUTHORS   Joh,T., Yamamoto,K., Kagami,Y., Kakuda,H., Sato,T., Yamamoto,T.,
            Takahashi,T., Ueda,R., Kaibuchi,K. and Seto,M.
  TITLE     Chimeric MLL products with a Ras binding cytoplasmic protein AF6
            involved in t(6;11) (q27;q23) leukemia localize in the nucleus
  JOURNAL   Oncogene 15 (14), 1681-1687 (1997)
   PUBMED   9349501
REFERENCE   8  (bases 1 to 7706)
  AUTHORS   Tanabe,S., Zeleznik-Le,N.J., Kobayashi,H., Vignon,C., Espinosa,R.
            3rd, LeBeau,M.M., Thirman,M.J. and Rowley,J.D.
  TITLE     Analysis of the t(6;11)(q27;q23) in leukemia shows a consistent
            breakpoint in AF6 in three patients and in the ML-2 cell line
  JOURNAL   Genes Chromosomes Cancer 15 (4), 206-216 (1996)
   PUBMED   8703846
REFERENCE   9  (bases 1 to 7706)
  AUTHORS   Saha,V., Lillington,D.M., Shelling,A.N., Chaplin,T., Yaspo,M.L.,
            Ganesan,T.S. and Young,B.D.
  TITLE     AF6 gene on chromosome band 6q27 maps distal to the minimal region
            of deletion in epithelial ovarian cancer
  JOURNAL   Genes Chromosomes Cancer 14 (3), 220-222 (1995)
   PUBMED   8589040
REFERENCE   10 (bases 1 to 7706)
  AUTHORS   Prasad,R., Gu,Y., Alder,H., Nakamura,T., Canaani,O., Saito,H.,
            Huebner,K., Gale,R.P., Nowell,P.C., Kuriyama,K. et al.
  TITLE     Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in
            acute myeloid leukemias with the t(6;11) chromosome translocation
  JOURNAL   Cancer Res 53 (23), 5624-5628 (1993)
   PUBMED   8242616
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL009178.4, AI271860.1,
            AB209420.1, AK055689.1, D60168.1 and BE503462.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001040000.2.
            
            Summary: This gene encodes a multi-domain protein involved in
            signaling and organization of cell junctions during embryogenesis.
            It has also been identified as the fusion partner of acute
            lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid
            leukemias with t(6;11)(q27;q23) translocation. Alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene, however, not all have been fully
            characterized.[provided by RefSeq, May 2011].
            
            Transcript Variant: This variant (2) has several differences
            compared to variant 4. The resulting isoform (2) is longer at the
            N-terminus but has a shorter and distinct C-terminus compared to
            isoform 4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB209420.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AL009178.4         12630-12709         c
            81-257              AI271860.1         1-177
            258-6892            AB209420.1         1-6635
            6893-7257           AK055689.1         1230-1594
            7258-7631           AK055689.1         1601-1974
            7632-7648           D60168.1           56-72               c
            7649-7706           BE503462.1         8-65                c
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q27"
     gene            1..7706
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="afadin, adherens junction formation factor"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
     exon            1..327
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..5178
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 4; myeloid/lymphoid
                     or mixed-lineage leukemia; translocated to, 4; ALL1-fused
                     gene from chromosome 6 protein; protein AF-6"
                     /codon_start=1
                     /product="afadin isoform 2"
                     /protein_id="NP_001035089.1"
                     /db_xref="CCDS:CCDS47517.1"
                     /db_xref="GeneID:4301"
                     /db_xref="HGNC:HGNC:7137"
                     /db_xref="MIM:159559"
                     /translation="MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMR
                     FYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGER
                     RLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRT
                     LSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNP
                     EVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADF
                     AVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPS
                     DKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSP
                     GRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHC
                     DLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAL
                     AKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASST
                     AERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTN
                     SSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQK
                     NIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNN
                     YMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWL
                     FNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMD
                     KYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELA
                     RSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTR
                     SPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQ
                     DKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLE
                     VAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQL
                     PWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGN
                     LCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDS
                     NHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQE
                     HLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGD
                     FDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRRE
                     QERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRD
                     LQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAE
                     ESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRR
                     QQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL"
     exon            328..523
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            524..636
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            637..800
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            801..961
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1119
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1231
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1399
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1444
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1539
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1802
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1872
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1991
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2053
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2259
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2405
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2406..2500
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2766
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2910
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2911..3109
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3110..3213
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3300
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3301..3366
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..3440
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..3559
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3754
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3755..3846
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3847..4032
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4033..5034
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     exon            5035..7706
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6871..6876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="hexamer: AATAAA"
     polyA_site      6893
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="major polyA site"
     regulatory      7683..7688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="hexamer: AATACA"
     polyA_site      7706
                     /gene="AFDN"
                     /gene_synonym="AF6; l-afadin; MLL-AF6; MLLT4"
                     /note="major polyA site"
ORIGIN      
        1 gcacaccggc cggcggggct gaggcggcgc ggcgcggcgc ggactgagcc ccaggcggag
       61 gccaagtcgg aggacgcggc gcggccgcgg aggcggaggc agccgcggag gcggaggcgg
      121 ccggcggggg gtggcgaggg gcgccgggcc cccgcggacc tgtcgtcctc ggcccgtcct
      181 ccggccccgg ccccgcgcgg ctgaggaggc gcggccagga ccatgtcggc gggcggccgt
      241 gacgaggagc ggcggaagct ggccgacatc atccaccact ggaacgccaa ccggctggac
      301 ctgttcgaga tcagccagcc gaccgaggat ttggagttcc atggagtgat gagattttat
      361 tttcaagata aagctgctgg aaactttgca acaaaatgta ttcgggtctc tagtactgcc
      421 accactcaag atgtaatcga aacgctcgcg gagaaatttc gacctgatat gcgaatgctg
      481 tcctctccca agtattcact ctatgaagtg catgtcagcg gagaaagaag attggatata
      541 gatgagaaac ctctagttgt acaactgaat tggaacaaag atgatcggga aggcagattt
      601 gttcttaaga atgagaatga cgccattcct cctaagaagg ctcaaagtaa tggacctgaa
      661 aagcaggaaa aagaaggggt tatccagaac ttcaagagaa ctctctcaaa gaaagaaaag
      721 aaggaaaaaa agaagagaga aaaagaggca ttgcgacagg catctgataa agatgataga
      781 cctttccaag gggaggatgt tgaaaattct cgactggctg ctgaggttta caaagacatg
      841 ccggaaacca gctttactcg aaccatttct aatcctgagg tggttatgaa acgacggagg
      901 cagcaaaaat tggaaaagag aatgcaggaa tttcggagct cagatgggcg gcctgattca
      961 ggtggaacat tgagaattta tgcagatagt ttaaaaccaa atattcccta caagacaatc
     1021 ctgctgtcta ctacagatcc tgcagacttt gctgtggctg aagctttaga gaagtatggt
     1081 ctggaaaaag aaaaccctaa ggattactgc atcgcccggg ttatgcttcc tcctggagcc
     1141 cagcattctg atgaaaaggg tgctaaagaa attattcttg atgatgatga gtgtccttta
     1201 caaatcttca gggaatggcc aagtgacaaa gggattttag tctttcagtt gaagaggagg
     1261 ccaccagacc acatcccaaa gaaaaccaag aaacacttgg aaggcaagac acccaaggga
     1321 aaggagagag ctgacgggtc tggctatggc tccacccttc ctccggagaa gctgccctat
     1381 ttagtagagt taagcccagg gagaaggaat cactttgcct actacaacta tcacacttac
     1441 gaagatggtt ctgactctag agataagcca aagctttacc gccttcagtt aagtgttact
     1501 gaagttggga cagaaaagtt ggatgacaac tctatccagt tgtttggccc aggaattcag
     1561 ccccatcact gtgaccttac caacatggat ggagtggtca ctgtgacgcc cagaagtatg
     1621 gacgcagaaa cctacgtgga aggccagcgc atctcagaaa ccaccatgct gcagagtggc
     1681 atgaaagtgc agtttggggc gtcccatgta tttaagtttg tggaccccag tcaggatcat
     1741 gctcttgcaa aaagatctgt ggatggaggc ctgatggtta agggcccaag acataaacct
     1801 ggaattgttc aggagacaac ttttgatttg ggaggagata ttcatagtgg gacagcatta
     1861 ccgacaagca agagcaccac taggctggac agcgacagag tgtcgtctgc ctctagcaca
     1921 gccgagcggg gaatggtgaa gccgatgatc agagtagaac agcagccaga ttatcgcagg
     1981 caagaaagca gaacacagga tgcttctggg cctgagctga tactacctgc aagcattgaa
     2041 ttcagggaaa gttctgaaga ttcatttttg tctgccatta taaattatac taatagctct
     2101 acagtccact ttaagttgtc ccctacatat gtattatata tggcatgccg gtatgtattg
     2161 tccaaccagt acagacctga catcagccct acagagcgca cacataaagt cattgcagtc
     2221 gtcaacaaga tggtgagcat gatggagggt gtcatccaga aacagaagaa tattgcaggg
     2281 gcacttgcct tctggatggc aaatgcatct gaacttctca acttcattaa gcaagaccga
     2341 gaccttagtc ggatcacact ggatgctcaa gatgttttag cacatttggt tcaaatggca
     2401 tttaaatact tggttcactg tcttcaatca gaacttaata attacatgcc agcctttcta
     2461 gatgaccctg aagagaacag tctgcaacga ccaaaaatag atgatgtgct gcacacgctc
     2521 acaggagcca tgtccttgct acgacgctgc agagtcaatg ccgccctgac catccagctc
     2581 ttctctcagc tcttccactt catcaatatg tggctgttca atagattggt gaccgaccca
     2641 gattcggggc tgtgctccca ttactggggt gcgattatcc gtcagcagtt gggccatatt
     2701 gaagcctggg ctgagaagca ggggctggaa ctggctgcgg actgtcatct gagcaggatc
     2761 gtgcaggcaa cgactttgct taccatggat aagtatgcac ctgatgacat tccaaatata
     2821 aacagcacct gctttaagtt aaattcatta caacttcaag ccttattaca gaactatcac
     2881 tgtgcacctg atgagccttt tatcccaacg gatcttatag aaaatgtagt gactgtggct
     2941 gaaaacactg ccgatgagct ggcccgcagt gatggaaggg aagtgcagtt ggaggaggat
     3001 cctgatctgc agctgccgtt tcttttgcca gaagatggtt attcttgtga tgttgtcaga
     3061 aacattccaa atggtttaca agaattttta gaccctctgt gccagagagg attttgcagg
     3121 ttaattcctc acacacgttc accaggtact tggacaatat attttgaagg tgcagattat
     3181 gaaagtcacc ttctgcgtga gaacacagag ctggctcagc ctctgaggaa agaacctgaa
     3241 ataatcactg tgaccctaaa aaagcagaat ggaatgggcc ttagcattgt tgcagcaaag
     3301 ggtgctggtc aagataaact aggaatctat gtgaagtcgg ttgtgaaagg aggtgctgca
     3361 gatgtggatg gacgtctagc tgcaggtgat cagctcctca gtgtggatgg acgaagtctg
     3421 gttggactct ctcaggaaag ggcggcagaa ctcatgacaa gaacaagctc tgtggtgaca
     3481 ctggaagtag caaagcaggg tgccatctac cacggtctgg ccacccttct caatcagcca
     3541 tcccccatga tgcagagaat ttcagatcgt cgtggctcag gtaaaccccg accaaagagt
     3601 gaaggctttg agctctataa taattcaact caaaatgggt ctcctgagag tcctcagctg
     3661 ccttgggcag aatatagtga accaaagaaa ttgcctggtg atgacagact gatgaaaaat
     3721 agagctgatc accgttccag ccccaacgta gcaaatcagc ctcctagtcc tggagggaaa
     3781 agtgcatatg cctctggaac aacagcgaag ataacatctg tctctactgg aaacctctgc
     3841 actgaggagc agacgcctcc gcctagacct gaagcctacc ccatccccac tcagacgtac
     3901 accagagagt attttacctt cccagcttcc aaatcccagg atcggatggc tcctcctcag
     3961 aaccagtggc caaattatga ggaaaagcca catatgcaca cagatagtaa tcattccagt
     4021 attgcaattc agcgtgttac acgttcccaa gaagaacttc gagaagataa agcttaccaa
     4081 cttgagcggc atcgaataga ggcagctatg gaccgaaagt ctgatagtga tatgtggata
     4141 aatcagagct cctcactgga ctccagtacc tctagccagg agcatctgaa ccattcctct
     4201 aagtcggtca cccctgcttc cacactgacc aaaagtggcc ctggccgttg gaaaacacca
     4261 gcagccatac cggccacccc tgtggccgtc tcccagccaa tccgaacaga cctgcctccg
     4321 ccacccccgc cacctccagt ccactatgcc ggtgatttcg atggaatgtc catggatttg
     4381 cctctcccac cacccccttc cgccaaccag atagggctgc cgtctgcgca ggtggctgct
     4441 gctgaacgga gaaagagaga agaacatcag cgttggtatg agaaggagaa ggcccgcctg
     4501 gaggaggagc gggagaggaa gcggagagag caggagagga agttgggcca gatgcgcact
     4561 cagtccttaa accctgctcc gttttctccc ctgactgcac agcagatgaa gcccgaaaag
     4621 ccttccacac tccagcggcc acaggaaaca gtcattcggg agctgcagcc tcagcagcag
     4681 ccccgcacga tcgagcgcag agacttgcag tacattacag tcagcaaaga ggagctttcc
     4741 tcgggggaca gtctgtcccc cgacccgtgg aagcgggacg ccaaggagaa gctggagaag
     4801 cagcagcaga tgcacatcgt ggacatgctg agcaaggaga tccaggagct ccagagcaaa
     4861 ccggaccgca gcgccgagga gagcgaccgg ctgcgcaagc tcatgctgga gtggcagttc
     4921 cagaagagac tccaggagtc gaagcagaag gacgaagatg acgaggagga ggaggacgat
     4981 gatgtggaca ccatgctgat catgcagcgc ctggaggctg aacgaagagc gagggacgag
     5041 gagcggaggc ggcagcagca gttagaagag atgcgcaagc gggaagcgga agaccgagcg
     5101 aggcaagagg aagagcgccg gcggcaggag gaggagcgaa caaaacgaga cgctgaagaa
     5161 aaggttatgg tcctttaagg gcagctagaa ttttaccaag ttagcctgaa cgtaatcgat
     5221 tggctggggc agagcgggct ggaagttctg tgtttcttaa gaagcacgag gcagagcagg
     5281 gcctggctcc cccagctttg tgattggacc tgcaacttta ccccatctgg cccacctacc
     5341 tctcttctgg actgtctcca gatcccctta tctgccaagt tttgtctcct tcaaacttct
     5401 gaactcttgg gcgtgtgtag cagtgagcct ctttgcaaag ggttcgtttc ctcgggcact
     5461 catctttact gaacatggcc cagcttgtca ttgtgaaggt gacattggtt cagtgattgc
     5521 ttaaatggca tgtggaccgt gggaagcagt aggagcgtag taagacagtt ggctgccatt
     5581 caacatttca aatagatggt ttactcaaat cactcacttc tggttgatgg taaagagagc
     5641 agatggctta gaaaacatga tacaggaaaa tttagtatgt aatccagtaa atatcctaag
     5701 ttatttattt tcatgtgtgt gtgtgtgttt aaatcaagta acccttttaa ttgaatttta
     5761 aaatgacagc gaattttgac attctgagaa atggggattt tgagatgttt ctctccatat
     5821 ctccctttgt cctgtgtgtg tggccgacgc cctctggatg aagaggctgt agccgttgag
     5881 ctccctgtgg gggcctgttt catcccgagg ggactgcgtg ttgctctgtg tggtttgaga
     5941 gaagctgctc ttctctgtta cttcccttct atgaaggtag aagctttcag tgttacaact
     6001 tctgatggcg gaaaatttag tttttattaa taattccttc ttggatagtc tagtgaacta
     6061 tattaataga acagtgtact gttcacagaa aagaacttgt agggagtttt ttgtagtgat
     6121 atgctctatt aggatacaga acaatctaag agtaagcttg atagacatga tgtgacttca
     6181 cttaaatgga gtagagtgaa taatgtaagg acactcacct ctgttgaaat tggtgatttc
     6241 tgcattttgt agacctttgc cctctaagtt tatcatcata ttatctgttg aatctgaaca
     6301 gcatttgaag tcctctcaaa gagtttgagc tttcaggatt aggttcccct gtgagtcttt
     6361 cacttgtgta atggtaagtc aacattgatg atctttctaa ggaaatcaga tttacaaaat
     6421 gctgagccgc ttgatgtcta agtgttggtg gtttttgctg ttgctttgct gttccatgaa
     6481 agcgctccct ggctgcttgg agggctgtgc ttttccaggt cagtgccagc ttggctgtcc
     6541 caggccgtgc acagtgccca ttgctatagc tgggcccagt cctggccacg ccggagcctc
     6601 ggcgggggca gctgggttgt cacggcagca cagtgcacat gcagagagga ccaggccatg
     6661 tttttcctgt gctgagaaac tcttgggtta tttaattttt ccaatgtgtt ttcataactc
     6721 atgattattt gaaatacact tgtgttagta actgcttcta taattaagtg gtcactgtaa
     6781 aaaggcgaat aacctatttt tatatcagaa tgtccaaaac tcatgggttt gctttgttat
     6841 agaaaaatgt gccattcatt tgttgggtag aataaagttg tcttgtaatt gtatctgctc
     6901 ttggtataac taaaagagga tattttgatt tcttcctaaa atgttaccaa gcaactgttc
     6961 ttcatgttaa tactaagatt cttgatagtt ccatttaaaa gccacaagaa gtcattctgg
     7021 tttattcctc actccaaggg aacacttact ttgtgtagac aagaagatga gagtctttcc
     7081 ttcaggattt ctaaacacag agcaagaaga ttcaatgaaa aattgggctc agggcagtaa
     7141 ttggggctgg catttattct taagagtttt ctccctcaaa aggtgaagga gtgacattgc
     7201 gtattcactc tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtagc
     7261 tctagaggga gtagggagaa aatacaggat gaaattgaaa acagggacta acaacttttc
     7321 actgaatgcc agcaagctct ctaaaattag ttttctttgg ttgaacaaat ttacaattca
     7381 ttgtacttca tttcacagca ttaaacattt actcgaggca gtttattctg tttgctcttt
     7441 catccccctt cttatttact tttttcttgt gctctaaaac ttttgtcaat ctttaacaaa
     7501 actgtgctgt tacctttttt aatgttaaaa gaattttaac actgtcaact gtagtttgtg
     7561 cagatgtatg tcagaaaaaa atgtacagta tgtgtcaaaa tatatttgaa tatcttgttg
     7621 ctgttgctgt tagttcctgg ctctctaatt ttctgtctgt tagggagttt cttaagattt
     7681 gaaatacaag ttaagaacta tggaaa
//
