LOCUS       NM_152406               6024 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens actin filament associated protein 1 like 1 (AFAP1L1),
            transcript variant 1, mRNA.
ACCESSION   NM_152406 XM_059702
VERSION     NM_152406.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ren,J.S., Bai,W., Ding,J.J., Ge,H.M., Wang,S.Y., Chen,X. and
            Jiang,Q.
  TITLE     Hypoxia-induced AFAP1L1 regulates pathological neovascularization
            via the YAP-DLL4-NOTCH axis
  JOURNAL   J Transl Med 21 (1), 651 (2023)
   PUBMED   37737201
  REMARK    GeneRIF: Hypoxia-induced AFAP1L1 regulates pathological
            neovascularization via the YAP-DLL4-NOTCH axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Sun,B., Ding,B., Chen,Y., Peng,C. and Chen,X.
  TITLE     AFAP1L1 promotes gastric cancer progression by interacting with
            VAV2 to facilitate CDC42-mediated activation of ITGA5 signaling
            pathway
  JOURNAL   J Transl Med 21 (1), 18 (2023)
   PUBMED   36631800
  REMARK    GeneRIF: AFAP1L1 promotes gastric cancer progression by interacting
            with VAV2 to facilitate CDC42-mediated activation of ITGA5
            signaling pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6024)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6024)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6024)
  AUTHORS   Wang,M., Han,X., Sun,W., Li,X., Jing,G. and Zhang,X.
  TITLE     Actin Filament-Associated Protein 1-Like 1 Mediates Proliferation
            and Survival in Non-Small Cell Lung Cancer Cells
  JOURNAL   Med Sci Monit 24, 215-224 (2018)
   PUBMED   29323101
  REMARK    GeneRIF: AFAP1L1 promotes cell proliferation, accelerates cell
            cycle progression, and prevents cell apoptosis in lung cancer
            cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6024)
  AUTHORS   Takahashi,R., Nagayama,S., Furu,M., Kajita,Y., Jin,Y., Kato,T.,
            Imoto,S., Sakai,Y. and Toguchida,J.
  TITLE     AFAP1L1, a novel associating partner with vinculin, modulates
            cellular morphology and motility, and promotes the progression of
            colorectal cancers
  JOURNAL   Cancer Med 3 (4), 759-774 (2014)
   PUBMED   24723436
  REMARK    GeneRIF: AFAP1L1 plays a role in the progression of colorectal
            cancer by modulating cell shape and motility and by inhibiting
            anoikis, presumably through interactions with vinculin-including
            protein complexes.
REFERENCE   7  (bases 1 to 6024)
  AUTHORS   Kajita,Y., Kato,T. Jr., Tamaki,S., Furu,M., Takahashi,R.,
            Nagayama,S., Aoyama,T., Nishiyama,H., Nakamura,E., Katagiri,T.,
            Nakamura,Y., Ogawa,O. and Toguchida,J.
  TITLE     The transcription factor Sp3 regulates the expression of a
            metastasis-related marker of sarcoma, actin filament-associated
            protein 1-like 1 (AFAP1L1)
  JOURNAL   PLoS One 8 (1), e49709 (2013)
   PUBMED   23326307
  REMARK    GeneRIF: Findings indicate a role for transcription factor Sp3 in
            sarcomas as a driver for expression of the metastasis-related gene
            AFAP1L1 (actin filament-associated protein 1-like 1).
REFERENCE   8  (bases 1 to 6024)
  AUTHORS   Comuzzie,A.G., Cole,S.A., Laston,S.L., Voruganti,V.S., Haack,K.,
            Gibbs,R.A. and Butte,N.F.
  TITLE     Novel genetic loci identified for the pathophysiology of childhood
            obesity in the Hispanic population
  JOURNAL   PLoS One 7 (12), e51954 (2012)
   PUBMED   23251661
REFERENCE   9  (bases 1 to 6024)
  AUTHORS   Furu,M., Kajita,Y., Nagayama,S., Ishibe,T., Shima,Y., Nishijo,K.,
            Uejima,D., Takahashi,R., Aoyama,T., Nakayama,T., Nakamura,T.,
            Nakashima,Y., Ikegawa,M., Imoto,S., Katagiri,T., Nakamura,Y. and
            Toguchida,J.
  TITLE     Identification of AFAP1L1 as a prognostic marker for spindle cell
            sarcomas
  JOURNAL   Oncogene 30 (38), 4015-4025 (2011)
   PUBMED   21516130
  REMARK    GeneRIF: AFAP1L1 has a role in the progression of spindle cell
            sarcomas and is a prognostic biomarker.
REFERENCE   10 (bases 1 to 6024)
  AUTHORS   Snyder,B.N., Cho,Y., Qian,Y., Coad,J.E., Flynn,D.C. and
            Cunnick,J.M.
  TITLE     AFAP1L1 is a novel adaptor protein of the AFAP family that
            interacts with cortactin and localizes to invadosomes
  JOURNAL   Eur J Cell Biol 90 (5), 376-389 (2011)
   PUBMED   21333378
  REMARK    GeneRIF: Data suggest that AFAP1L1 may forge unique protein
            interactions in which AFAP1 is less efficient, and these
            interactions may allow AFAP1L1 to affect invadosome formation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA792192.1, BC125093.1, AK074185.1, AC131025.1 and BX107758.1.
            
            On Nov 23, 2018 this sequence version replaced NM_152406.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK074185.1, SRR1803613.172483.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296721.9/ ENSP00000296721.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                DA792192.1         22-81
            61-2508             BC125093.1         1-2448
            2509-2910           AK074185.1         2473-2874
            2911-2911           AC131025.1         121578-121578       c
            2912-4189           AK074185.1         2876-4153
            4190-5721           AC131025.1         118768-120299       c
            5722-6024           BX107758.1         1-303
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q32"
     gene            1..6024
                     /gene="AFAP1L1"
                     /note="actin filament associated protein 1 like 1"
                     /db_xref="GeneID:134265"
                     /db_xref="HGNC:HGNC:26714"
                     /db_xref="MIM:614410"
     exon            1..126
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..2417
                     /gene="AFAP1L1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     AFAP1-like protein 1"
                     /codon_start=1
                     /product="actin filament-associated protein 1-like 1
                     isoform 1"
                     /protein_id="NP_689619.1"
                     /db_xref="CCDS:CCDS34274.1"
                     /db_xref="GeneID:134265"
                     /db_xref="HGNC:HGNC:26714"
                     /db_xref="MIM:614410"
                     /translation="MDRGQVLEQLLPELTGLLSLLDHEYLSDTTLEKKMAVASILQSL
                     QPLPAKEVSYLYVNTADLHSGPSFVESLFEEFDCDLSDLRDMPEDDGEPSKGASPELA
                     KSPRLRNAADLPPPLPNKPPPEDYYEEALPLGPGKSPEYISSHNGCSPSHSIVDGYYE
                     DADSSYPATRVNGELKSSYNDSDAMSSSYESYDEEEEEGKSPQPRHQWPSEEASMHLV
                     RECRICAFLLRKKRFGQWAKQLTVIREDQLLCYKSSKDRQPHLRLALDTCSIIYVPKD
                     SRHKRHELRFTQGATEVLVLALQSREQAEEWLKVIREVSKPVGGAEGVEVPRSPVLLC
                     KLDLDKRLSQEKQTSDSDSVGVGDNCSTLGRRETCDHGKGKKSSLAELKGSMSRAAGR
                     KITRIIGFSKKKTLADDLQTSSTEEEVPCCGYLNVLVNQGWKERWCRLKCNTLYFHKD
                     HMDLRTHVNAIALQGCEVAPGFGPRHPFAFRILRNRQEVAILEASCSEDMGRWLGLLL
                     VEMGSRVTPEALHYDYVDVETLTSIVSAGRNSFLYARSCQNQWPEPRVYDDVPYEKMQ
                     DEEPERPTGAQVKRHASSCSEKSHRVDPQVKVKRHASSANQYKYGKNRAEEDARRYLV
                     EKEKLEKEKETIRTELIALRQEKRELKEAIRSSPGAKLKALEEAVATLEAQCRAKEER
                     RIDLELKLVAVKERLQQSLAGGPALGLSVSSKPKSGETANKPQNSVPEQPLPVNCVSE
                     LRKRSPSIVASNQGRVLQKAKEWEMKKT"
     exon            127..255
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            256..339
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            340..437
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            438..546
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            547..645
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            646..857
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1037
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1130
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1224
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1377
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1589
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1735
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1808
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1920
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2085
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..2264
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2393
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..6024
                     /gene="AFAP1L1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3333..3338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1L1"
                     /note="hexamer: AGTAAA"
     polyA_site      3353
                     /gene="AFAP1L1"
                     /note="major polyA site"
     regulatory      3517..3522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1L1"
                     /note="hexamer: AATAAA"
     regulatory      3523..3528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1L1"
                     /note="hexamer: GATAAA"
     polyA_site      3537
                     /gene="AFAP1L1"
                     /note="major polyA site"
     polyA_site      3544
                     /gene="AFAP1L1"
     regulatory      4153..4158
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1L1"
                     /note="hexamer: ATTAAA"
     polyA_site      4192
                     /gene="AFAP1L1"
                     /note="major polyA site"
     polyA_site      4295
                     /gene="AFAP1L1"
     regulatory      6002..6007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1L1"
                     /note="hexamer: AATAAA"
     polyA_site      6024
                     /gene="AFAP1L1"
ORIGIN      
        1 agagagcgcc ggccgctggg ctggcctgag agcgcagcgc gccggccgct accagccgcg
       61 ccggagcccc tgcgccctgc ggcccgctcc ccggggaccg ggccggcgcc atggaccgag
      121 gccaggtgct ggagcagctg ctcccagagc tcaccgggct gctcagcctc ctggaccacg
      181 agtacctcag cgataccacc ctggaaaaga agatggccgt ggcctccatc ctgcagagcc
      241 tgcagcccct tccagcaaag gaggtctcct acctgtatgt gaacacagca gacctccact
      301 cggggcccag cttcgtggaa tccctctttg aagaatttga ctgtgacctg agtgaccttc
      361 gggacatgcc agaggatgat ggggagccca gcaaaggagc cagccctgag ctagccaaga
      421 gcccacgcct gagaaacgcg gccgacctgc ctccaccgct ccccaacaag cctccccctg
      481 aggactacta tgaagaggcc cttcctctgg gacccggcaa gtcgcctgag tacatcagct
      541 cccacaatgg ctgcagcccc tcacactcga ttgtggatgg ctactatgag gacgcagaca
      601 gcagctaccc tgcaaccagg gtgaacggcg agcttaagag ctcctataat gactctgacg
      661 caatgagcag ctcctatgag tcctacgatg aagaggagga ggaagggaag agcccgcagc
      721 cccgacacca gtggccctca gaggaggcct ccatgcacct ggtgagggaa tgcaggatat
      781 gtgccttcct gctgcggaaa aagcgtttcg ggcagtgggc caagcagctg acggtcatca
      841 gggaggacca gctcctgtgt tacaaaagct ccaaggatcg gcagccacat ctgaggttgg
      901 cactggatac ctgcagcatc atctacgtgc ccaaggacag ccggcacaag aggcacgagc
      961 tgcgtttcac ccagggggct accgaggtct tggtgctggc actgcagagc cgagagcagg
     1021 ccgaggagtg gctgaaggtc atccgagaag tgagcaagcc agttggggga gctgagggag
     1081 tggaggtccc cagatcccca gtcctcctgt gcaagttgga cctggacaag aggctgtccc
     1141 aagagaagca gacctcagat tctgacagcg tgggtgtggg tgacaactgt tctacccttg
     1201 gccgccggga gacctgtgat cacggcaaag ggaagaagag cagcctggca gaactgaagg
     1261 gctcaatgag cagggctgcg ggccgcaaga tcacccgtat cattggcttc tccaagaaga
     1321 agacactggc cgatgacctg cagacgtcct ccaccgagga ggaggttccc tgctgtggct
     1381 acctgaacgt gctggtgaac cagggctgga aggaacgctg gtgccgcctg aagtgcaaca
     1441 ctctgtattt ccacaaggat cacatggacc tgcgaaccca tgtgaacgcc atcgccctgc
     1501 aaggctgtga ggtggccccg ggctttgggc cccgacaccc atttgccttc aggatcctgc
     1561 gcaaccggca ggaggtggcc atcttggagg caagctgttc agaggacatg ggtcgctggc
     1621 tcgggctgct gctggtggag atgggctcca gagtcactcc ggaggcgctg cactatgact
     1681 acgtggatgt ggagacctta accagcatcg tcagtgctgg gcgcaactcc ttcctatatg
     1741 caagatcctg ccagaatcag tggcctgagc cccgagtcta tgatgatgtt ccttatgaaa
     1801 agatgcagga cgaggagccc gagcgcccca caggggccca ggtgaagcgt cacgcctcct
     1861 cctgcagtga gaagtcccat cgtgtggacc cgcaggtcaa agtcaaacgc cacgcctcca
     1921 gtgccaatca atacaagtat ggcaagaacc gagccgagga ggatgcccgg aggtacttgg
     1981 tagaaaaaga gaagctggag aaagagaaag agacgattcg gacagagctg atagcactga
     2041 gacaggagaa gagggaactg aaggaagcca ttcggagcag cccaggagca aaattaaagg
     2101 ctctggaaga agccgtggcc accctggaag ctcagtgtcg ggcaaaggag gagcgccgga
     2161 ttgacctgga gctgaagctg gtggctgtga aggagcgctt gcagcagtcc ctggcaggag
     2221 ggccagccct ggggctctcc gtgagcagca agcccaagag tggggaaact gcaaataaac
     2281 cccagaacag cgttccagag caacctctcc ctgtcaactg tgtttctgag ctgaggaaga
     2341 ggagcccatc catcgtagcc tccaaccaag gaagggtgct acagaaagcc aaggaatggg
     2401 aaatgaagaa gacctaggaa gaggatgagg atttcattcc aaaggaaata ccagcttgtc
     2461 cacctgagga agaaatgctt ttttcacaag gagacaccaa gagaagacta ggaagtagcc
     2521 ctcgttctcc agggcaccca aaataccagc ctttattgtc tgcatgattt taggggatat
     2581 ggggagggaa caagtagaag ggaagaggga aatggagagc atccttatga ctttacaaag
     2641 ggtggaaatg aggatggagg gatacagaag tctgcacagc tgtaaaggtt ttatagatgt
     2701 ctttgccttc ccttctgagg aagggaagaa gtaatgaaag cacatgtgaa taaccccttc
     2761 catcccattc acagcatcgc actcccagtc cttaaggcaa agggaggcag tgctgaagca
     2821 ttggtggtgc agtgtaaaga gacaagacct gatcatctga tcacacttgt gccaacttga
     2881 ttcatattgg gcattactaa caacccctgg tcaaggtaaa taggttgaac aatcaataac
     2941 attatccctg cctgcataca tgtgaacaaa agctatagag gacatgcaaa ttctacagtc
     3001 attcctcata tgctttagac agagtgcagc tactggaatc ttccagattt cagtgtttta
     3061 aaatcagagc tctgaataca caaaaggaaa gagaaatgga gcagctgaca tattttaagc
     3121 tcacagtgat actcagtgac aggagcacag agctctaatg tccacaggat gttgtagggt
     3181 agggtctctc agtaaatcaa gtcccttacc tatgttctga cactgaggct cttggagcta
     3241 tgggttagaa atccaggagg caatatgtct ttattctaat gaagtcctca tcttgcactc
     3301 agaggcccac tagtttgccc ttctatatat taagtaaaac caagagaaat taagaggatg
     3361 atggcttctt tctgtcacat gggtgctaaa ccttttacaa acacttgata cttctataga
     3421 gggaccaagg acctcaagtc ctgggcagtc tccttactgt gctactcact actgtctcaa
     3481 cactgtagtg agtctgtctt taacacattt ccttccaata aagataaaac cacttaactc
     3541 ttaagtgcca gtgcttagct caagaggaaa gaaggaaaat cccagaataa ccgtggccac
     3601 tttgctatag gggttacata ttttaaccca cctaaggttt tacagacttt agcctcactc
     3661 aaactttatc accctctgag gtagctggag accacagcag gaaaaataac ctgaccagta
     3721 atcacccact gattagtggc agagcgtagg acttaggggt tataagccca gatccagata
     3781 gggctaggtt tgagccatgc ctctaatgac cagctgggtg accttgggaa agtcacttga
     3841 ttgctctgat tcttgttttc cttatctgtg aaatgggact aacaattgtt acttttctcc
     3901 caagattgtt gggaagattt aataagataa tatatagtag gcatcttgca tagtgtctga
     3961 gaaataagaa atgctcagtc aatagtggct ctcattagcc aggcatggtg gtactcgcct
     4021 gtagtcccag ctactcagga ggctgaggca ggaggaccgc ttgagcccag gagtttgaga
     4081 ctgtagtgca ctatgatcac gcctgtgaat tgccactgca ctccagccta agcaaatagc
     4141 aagaccccat ctattaaaaa aaaaaagtgg ctattattag ctatatcaga aatgtctgac
     4201 tcacaagcct gtgttctttc cactgaagag gaaattactt ttcagtgttc ttttttaaaa
     4261 cgtggatcct gaaatgtcac ctaactctcc aactatgtct tcagtgtctg tcatactctt
     4321 tcttatgcag ttattttggg gttaagtgtc ttttctcacc aacagatgaa ctctcagagg
     4381 agtggctggg ggtaattaat aattaacatt tatcaagcac tcaccatatg tcaggcactg
     4441 tgtcagccat ggccagcttc acaggcatgc aacctgtgca gtcacacagg gccccatgct
     4501 tagaagggcc ctgttcttgg tttaatgctc tactattgcc ttcttaatca tatttgaaca
     4561 aggagttctg cttttccttt tgccctttaa attagatagc cagtcctggt gctaactaac
     4621 cacttttcta ggtattatcc ctttagtttt cactgaaacc ctgagataag ttatattgcc
     4681 ctagttttca cagttcagga aatggaagct cacagaggtc aggtaacttg tccaagctcc
     4741 catagttctt atatttacat gctggtggag ccagcatgtg aatataagaa gtctgaccct
     4801 agagccttat tcttaatttt aaagctctat gtcatccata tatctccctc cccttgtact
     4861 gcaacattca gaagcacact agcacttaat aaatagacga atgaatgcat ggatgcaagc
     4921 agcaacctgg tgaacgttag gcagctagga gaaatgtaag caaaatggct aaggcacaga
     4981 tgccacttgt tcttggttgt taaaatgcct tgtttaggcc aggtatggtg gctcatgcct
     5041 gtaatcccag cactttggga ggccgaagcg ggtggatcac ttgaggtcag gagttcaaga
     5101 ccagcctggc caacatggca aaaccccatc tctatcaaaa atacaaaagt tagctgggca
     5161 cggtggcaca tgccggtagt cccagctact tgggaggctg agggaggaga atcacttgaa
     5221 cccgggaggt ggaggttgca gtgagccgag atcgcaccac tgcactccag cctgggcaat
     5281 agcaagactc catctcaaaa aaaaaaaaat gccttatttg gttgtgtttt ttcaaagtgt
     5341 aagccacagg tctaagccca acactgccaa gtgttcagtc tgtcagaatt ctactgtcac
     5401 tgtttcctta ttgcgggatc ttgcataggt ttaccaacct ttctgagcct caattttctc
     5461 atatgtaaaa gagaatcata atacctccct aaaccatatc acccttttca taaggccaaa
     5521 aagaaaatat aagttattgg tcatgacaca tttgagttgg acctcggatt agataaaaag
     5581 atgaaaatac tccagctctg tcctcatgga actcagagac aagcaggaaa gaaaatgtga
     5641 cccaaataac tacagtacaa ataacccatg ttatgacaag attcagagtg ctcaaggaac
     5701 acttcccatt cccttaaaaa aaaaaattaa gggactatcc agtaactaag ctggcaactc
     5761 caagtgccct ggaccccagt caccaatcca tcctctcagt ctgtcagctt tccatcctaa
     5821 atctctaaaa ttcatccata tcttcctatc ttcactgaca ctatccaact taattaagtc
     5881 attcaacaaa tacttgagta cctactatac tagatgtcag aaatcagtgg tgaacaagac
     5941 agacacctca tgaagctcac attctagttg gaggaaaaaa ccttaacaag gaagcaaaag
     6001 aaataaaata ctaagaaact aaaa
//
