LOCUS       NM_001134647            7704 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens actin filament associated protein 1 (AFAP1),
            transcript variant A, mRNA.
ACCESSION   NM_001134647
VERSION     NM_001134647.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Fan,H., Dong,Q., Yu,J., Yin,L., Sun,X., Yang,X. and Zhang,W.
  TITLE     [Knockdown of long non-coding RNA actin filament-associated protein
            1 antisense RNA1 (AFAP1-AS1) inhibits epithelial-mesenchymal
            transition, invasion and migration of TPC-1 papillary thyroid
            cancer cells]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 37 (1), 54-60 (2021)
   PUBMED   33441229
  REMARK    GeneRIF: [Knockdown of long non-coding RNA actin
            filament-associated protein 1 antisense RNA1 (AFAP1-AS1) inhibits
            epithelial-mesenchymal transition, invasion and migration of TPC-1
            papillary thyroid cancer cells].
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Yuan,X.H., Li,J., Cao,Y., Jie,Z.G. and Zeng,Y.F.
  TITLE     Long non-coding RNA AFAP1-AS1 promotes proliferation and migration
            of gastric cancer by downregulating KLF2
  JOURNAL   Eur Rev Med Pharmacol Sci 24 (2), 673-680 (2020)
   PUBMED   32016968
  REMARK    GeneRIF: Long non-coding RNA AFAP1-AS1 promotes proliferation and
            migration of gastric cancer by downregulating KLF2.
REFERENCE   3  (bases 1 to 7704)
  AUTHORS   Geng,Y.D., Wang,S.B., Lu,T.Q. and Teng,W.
  TITLE     [Expression and functions of long non-coding RNA actin
            filament-associated protein 1-antisense RNA1 in oral squamous cell
            carcinoma]
  JOURNAL   Hua Xi Kou Qiang Yi Xue Za Zhi 37 (6), 594-601 (2019)
   PUBMED   31875436
  REMARK    GeneRIF: The expression of AFAP1-AS1 was high in the oral squamous
            cell carcinoma tissues and SCC25 cells and is associated with the
            development and prognosis of oral squamous cell carcinoma
REFERENCE   4  (bases 1 to 7704)
  AUTHORS   Lian,Y., Xiong,F., Yang,L., Bo,H., Gong,Z., Wang,Y., Wei,F.,
            Tang,Y., Li,X., Liao,Q., Wang,H., Zhou,M., Xiang,B., Wu,X., Li,Y.,
            Li,X., Chen,X., Li,G., Guo,C., Zeng,Z. and Xiong,W.
  TITLE     Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of
            miR-423-5p to facilitate nasopharyngeal carcinoma metastasis
            through regulating the Rho/Rac pathway
  JOURNAL   J Exp Clin Cancer Res 37 (1), 253 (2018)
   PUBMED   30326930
  REMARK    GeneRIF: Authors determined that AFAP1-AS1 functions as a competing
            endogenous RNA in NPC to regulate the Rho/Rac pathway through
            miR-423-5p.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7704)
  AUTHORS   Hao,F., Mou,Y., Zhang,L., Wang,S. and Yang,Y.
  TITLE     LncRNA AFAP1-AS1 is a prognostic biomarker and serves as oncogenic
            role in retinoblastoma
  JOURNAL   Biosci Rep 38 (3) (2018)
   PUBMED   29654169
  REMARK    GeneRIF: e experiment in vitro suggested down-regulation of
            AFAP1-AS1 inhibited retinoblastoma cell proliferation, migration
            and invasion, and blocked cell cycle. In conclusion, AFAP1-AS1
            functions as an oncogenic lncRNA in retinoblastoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7704)
  AUTHORS   Baisden,J.M., Gatesman,A.S., Cherezova,L., Jiang,B.H. and
            Flynn,D.C.
  TITLE     The intrinsic ability of AFAP-110 to alter actin filament integrity
            is linked with its ability to also activate cellular tyrosine
            kinases
  JOURNAL   Oncogene 20 (45), 6607-6616 (2001)
   PUBMED   11641786
REFERENCE   7  (bases 1 to 7704)
  AUTHORS   Baisden,J.M., Qian,Y., Zot,H.M. and Flynn,D.C.
  TITLE     The actin filament-associated protein AFAP-110 is an adaptor
            protein that modulates changes in actin filament integrity
  JOURNAL   Oncogene 20 (44), 6435-6447 (2001)
   PUBMED   11607843
  REMARK    Review article
REFERENCE   8  (bases 1 to 7704)
  AUTHORS   Qian,Y., Baisden,J.M., Zot,H.G., Van Winkle,W.B. and Flynn,D.C.
  TITLE     The carboxy terminus of AFAP-110 modulates direct interactions with
            actin filaments and regulates its ability to alter actin filament
            integrity and induce lamellipodia formation
  JOURNAL   Exp Cell Res 255 (1), 102-113 (2000)
   PUBMED   10666339
REFERENCE   9  (bases 1 to 7704)
  AUTHORS   Guappone,A.C., Weimer,T. and Flynn,D.C.
  TITLE     Formation of a stable src-AFAP-110 complex through either an
            amino-terminal or a carboxy-terminal SH2-binding motif
  JOURNAL   Mol Carcinog 22 (2), 110-119 (1998)
   PUBMED   9655255
REFERENCE   10 (bases 1 to 7704)
  AUTHORS   Qian,Y., Baisden,J.M., Westin,E.H., Guappone,A.C., Koay,T.C. and
            Flynn,D.C.
  TITLE     Src can regulate carboxy terminal interactions with AFAP-110, which
            influence self-association, cell localization and actin filament
            integrity
  JOURNAL   Oncogene 16 (17), 2185-2195 (1998)
   PUBMED   9619827
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB450895.1, BI561136.1,
            AK290807.1, AK297631.1, AK127962.1, AC112254.6, AC004169.3 and
            CA448961.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001134647.1.
            
            Summary: The protein encoded by this gene is a Src binding partner.
            It may represent a potential modulator of actin filament integrity
            in response to cellular signals, and may function as an adaptor
            protein by linking Src family members and/or other signaling
            proteins to actin filaments. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Aug 2008].
            
            Transcript Variant: This variant (A) differs in the 5' UTR and
            includes an alternate in-frame exon in the coding region compared
            to variant 4. The variant encodes a longer protein, isoform A,
            compared to isoform B.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372080.713210.1, AK297631.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000420658.6/ ENSP00000410689.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                DB450895.1         66-147
            83-108              BI561136.1         146-171
            109-1600            AK290807.1         1-1492
            1601-1889           AK297631.1         1459-1747
            1890-1929           AK127962.1         677-716
            1930-1930           AC112254.6         10417-10417         c
            1931-2794           AK297631.1         1789-2652
            2795-7692           AC004169.3         9397-14294
            7693-7704           CA448961.1         18-29               c
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p16.1"
     gene            1..7704
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /note="actin filament associated protein 1"
                     /db_xref="GeneID:60312"
                     /db_xref="HGNC:HGNC:24017"
                     /db_xref="MIM:608252"
     exon            1..206
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            207..335
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..2653
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /note="isoform A is encoded by transcript variant A; actin
                     filament-associated protein, 110 kDa; 110 kDa actin
                     filament-associated protein"
                     /codon_start=1
                     /product="actin filament-associated protein 1 isoform A"
                     /protein_id="NP_001128119.1"
                     /db_xref="CCDS:CCDS47010.1"
                     /db_xref="GeneID:60312"
                     /db_xref="HGNC:HGNC:24017"
                     /db_xref="MIM:608252"
                     /translation="MEELIVELRLFLELLDHEYLTSTVREKKAVITNILLRIQSSKGF
                     DVKDHAQKQETANSLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSP
                     GKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFL
                     LRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCNITYIPKDSKKKKHELK
                     ITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDSECPPPPSSPVHKAELEKKL
                     SSERPSSDGEGVVENGITTCNGKEQVKRKKSSKSEAKGTVSKVTGKKITKIISLGKKK
                     PSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDNKLIFHKDRTDLKTHIVSIPL
                     RGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPEALH
                     YDYIDVEMSASVIQTAKQTFCFMNRRVISANPYLGGTSNGYAHPSGTALHYDDVPCIN
                     GSWEPEDGFPASCSRGLGEEVLYDNAGLYDNLPPPHIFARYSPADRKASRLSADKLSS
                     NHYKYPASAQSVTNTSSVGRASLGLNSQLKGKKPPVASNGVTGKGKTLSSQPKKADPA
                     AVVKRTGSNAAQYKYGKNRVEADAKRLQTKEEELLKRKEALRNRLAQLRKERKDLRAA
                     IEVNAGRKPQAILEEKLKQLEEECRQKEAERVSLELELTEVKESLKKALAGGVTLGLA
                     IEPKSGTSSPQSPVFRHRTLENSPISSCDTSDTEGPVPVNSAAVLKKSQAAPGSSPCR
                     GHVLRKAKEWELKNGT"
     exon            336..433
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            434..542
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            543..754
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            755..934
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1030
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1112
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1262
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1474
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1620
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1738
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1990
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2105
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2270
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2461
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2626
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     exon            2627..7704
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7686..7691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /note="hexamer: AATAAA"
     polyA_site      7704
                     /gene="AFAP1"
                     /gene_synonym="AFAP; AFAP-110; AFAP110"
                     /note="major polyA site"
ORIGIN      
        1 gtctgtcccg gccgcagccc cacgccggct gcagctccgc gagccgagcc ggatctcgag
       61 cggggtacac gggctgaggc ggcggcggcg gccccgcccc ggggctccat tgttaaggcg
      121 gctgcggctc tgtcggctgt tcagctgcgc tccagtcggc ccaggcgccg cggcgaggag
      181 cgagcgctgc gaggtggcgg cctgagcaat ggaagagtta atagttgaac ttcgtctctt
      241 tcttgaactc ctggaccatg aatatctaac ctcaactgtc agggagaaaa aggcagtgat
      301 aaccaacatt ctgctaagaa tacagtcatc caaaggtttt gatgtgaagg accatgctca
      361 gaagcaggag accgctaaca gcctgccagc ccctcctcag atgcccctgc cggagatccc
      421 tcagccctgg ctgcctcctg acagtgggcc tccaccattg ccaacatcct ccctcccaga
      481 aggttattat gaggaagctg tgccgctgag ccccggaaaa gctccggaat acatcacatc
      541 aaattatgat tccgatgcga tgagcagctc ttatgagtcg tatgatgaag aggaggagga
      601 tgggaagggg aagaaaaccc ggcaccagtg gccctccgag gaggcctcca tggacctggt
      661 caaggacgcc aaaatctgcg ccttcctgct gcggaagaag cggttcggcc agtggaccaa
      721 gttgctctgc gtcatcaaag acaccaaact gctgtgctat aaaagttcca aggaccagca
      781 gcctcagatg gaactgccac tccaaggctg taacattacg tacatcccga aagacagcaa
      841 aaagaagaag cacgagctga agattactca gcagggcacg gacccgcttg ttctcgccgt
      901 ccagagcaag gaacaggccg agcagtggct gaaggtgatc aaagaagcct acagtggttg
      961 tagtggcccc gtggattcag agtgtcctcc tccaccaagc tccccggtgc acaaggcaga
     1021 actggagaag aaactgtctt cagagagacc cagctcagat ggggagggtg ttgtggaaaa
     1081 tggaattacc acatgtaatg gaaaggagca agtgaagagg aagaaaagtt ccaaatcaga
     1141 ggccaagggc actgtgtcga aagtcactgg gaaaaaaatc accaagatca tcagtctggg
     1201 aaagaaaaag ccgtccacag acgagcagac ctcctcagct gaggaagatg ttcccacctg
     1261 cggctatctg aacgtgctct ccaacagccg ctggcgagag cgctggtgcc gagtgaaaga
     1321 taacaagctc attttccaca aggacaggac cgacctgaag acccatattg tgtctattcc
     1381 gctccgtggc tgcgaggtga tcccgggttt ggattctaaa catcctctga cgttccggct
     1441 gctgcgcaac ggccaggagg ttgcagtatt ggaggcatct tcttctgaag acatgggcag
     1501 gtggattggg attttactcg cagagacggg atcgtccaca gacccggagg ctctgcacta
     1561 tgactacatt gatgtggaga tgtctgcaag tgtcattcag acagccaaac agaccttctg
     1621 tttcatgaac aggcgtgtta tatctgctaa cccatatcta gggggcacct ccaacggcta
     1681 tgcccacccc agcgggacgg cacttcatta tgacgatgtc ccgtgcatca acggctcgtg
     1741 ggaaccggaa gacggctttc ctgcttcctg cagcagaggc ttgggagaag aggtgcttta
     1801 tgataacgca ggcctgtacg ataacttgcc gcctccgcac atctttgccc gctactctcc
     1861 tgctgacaga aaggcctcta ggctgtctgc tgacaagctg tcctctaacc attacaaata
     1921 ccctgcctcc gctcagtctg tcactaatac ctcttctgtg gggagggcgt ctctcgggct
     1981 caactcgcag ctcaagggta aaaagccccc cgtggcgtct aatggggtca caggaaaagg
     2041 gaagactctg agcagtcagc caaagaaagc ggatcccgcg gctgttgtga aaaggacggg
     2101 ttcgaatgct gcccagtaca agtatggcaa gaaccgggta gaagcagatg ccaagcggct
     2161 acagaccaaa gaggaggagc tgctgaagag gaaagaggcc ctgcggaata ggctggccca
     2221 gctccgcaag gaaagaaaag accttcgagc ggctattgaa gtgaacgccg gcaggaagcc
     2281 gcaggcgatc ctggaggaga agctgaagca gctggaggag gagtgccggc agaaggaggc
     2341 ggagcgtgtc agcctggagc tggagctgac ggaggtcaag gagagcctga agaaagcgct
     2401 ggcgggcgga gtcaccctgg ggctggccat cgagcccaag tcagggacat cgagtccaca
     2461 gtctccagtg ttccggcacc ggaccctgga aaactcgccc atctccagct gtgacaccag
     2521 tgacaccgag ggccccgtgc cggtgaacag cgcggccgtc ttgaagaaga gccaggctgc
     2581 cccgggcagc tccccctgcc gagggcatgt gctgcggaag gccaaggaat gggaattgaa
     2641 gaacgggacc taggggacag cagcaccact ccagcctcag agactgcaca cccccttgcc
     2701 tgtatcctca tctgtgtgac ggcaggaagc tctgcccaga gtggcctcag ctgcacgact
     2761 ccagaggctc cacgactgag ctctgaggcc agtgcctgtc ccccaggccc acttgtattc
     2821 tttctactgt aaaatggcgc ctttaaaaaa gatgtaagac tttggttccc aactttgtta
     2881 aaaaacaaaa aaagtgatgg aaaggtgttt gaaaggatgc tatcaggcga agtgacatct
     2941 ttctaaccag ataggccatt gctttcaccg attccaaccc agccctggac tgaggatgga
     3001 tggttccatg agcccccaga tgaaaacctg gattcgacag ggaagagctt ggatccccgg
     3061 ctgccccggg catgagtgtg gctggccatg ggcactgggc tcgccttgca cgcccaccag
     3121 gagggccagg ggcatacgca gtcctgacgt ggactttgtg tcttggcgta ccctctccac
     3181 gtgcccgtcc ccctcgtcat cagaggtaaa ggcatgagaa aatcgcgctg tgaaacgttt
     3241 tttacctttt attattattt tttggacaga tgcttttgtc accaagacat gaaaagctgc
     3301 cattcttctt aacctgtttt ttaggaatgt ctttttactg tgtgtcacac ttagatcaag
     3361 gagttttcag aggttcctta ttttattaat taattatttg tgctgctttt gacaggtagc
     3421 agagggtttt aatacttcac taacagtaca ccctcctccc ccttgggcta gcatccccag
     3481 cccttaatcc acgtcggagc cggcaggtgc ttctcacacc aggagcctct gggggcaggg
     3541 gctggctcct cgccgctgta caggacgttt actgctgctt ggggccaggg cagtcctttg
     3601 aaagggaagc aagttccgga atttgttcgc tcatggaact ataacacctt agagcaagag
     3661 gcaggaattc cagggcctcc tgcccctccc cttcctccag cttgtcaagt taaaccacta
     3721 atgtgttggt gggcctggcc gtgctgcctc tcctgagtag ggtgggtgca agtagggcct
     3781 ctttcccagc tttcttgctt gccttctgtg caatatttct tggagaattt ggatgcctaa
     3841 ggcataaagg aaaaaaacag agttcatagt ttgagctggt atttaccccc tgggtttaaa
     3901 aatcagatta acttcttggt ctcccggagg ctgctgtcag gaattctcca gtacgggaag
     3961 atgcagtcac ttatgacgaa ataagtcacc cctgaacgcc tccgcaggca gattcatgga
     4021 gaccagtccc agtagcaacc gccccttccc caagcataga atacagaggt gccatacttg
     4081 gtgtaagcct tccttctgtc cactgtccag aatgtccttt ttcacacaga ggaagagatg
     4141 gcgggaaaac actgactgac tccttgggtt tcccccagac atactttcag agcgctagct
     4201 gtgtggtgca cgggacttta cagttaggat tcatgtttaa ggtggtggcg cttgaggcct
     4261 gcaagggcca cgttggatta ctgaagcgga aagcggtctc ccatgtataa gaaaaagttt
     4321 tacaacacga attggaagct gcttgagtcc acctgaactt gaattgattg caaagccatc
     4381 tgctgggaac ggttttgggt tcctgagcca tcagccagcc tttctttccc ctctgatgca
     4441 cgttcctccc ggcctagtcc gtggaggccc atcacaccag catccgtgac gccagcttcc
     4501 cactaggaca tccaggcagg tcgggcgggg acgccacaca cctcagggcc gtccgctgca
     4561 ttgccataga ggagccctcc ctccagtggt cacttcccac cattgcaggc agacttccca
     4621 ccattgcagg cagctgggcc cacttcatat cacacaaaat gaattttcta taaagggaaa
     4681 aaagtttgct cctccataga tttttgctgc cttcgggtca aatagtttcc ctggttgaca
     4741 ctcttgggcc actctgattg gcagcctgct ttgccaaaga gaactgtgtg aggttttggt
     4801 ggttagtaag agaaggggga gatggccagc ctgaagtcac ggtcctggcc cagcacaggt
     4861 gggcagcagc gagctggcag gtgaagtgtg agcagcacgc aaagtgtcac gtgggcctta
     4921 gccgtcaccc accagcttcc tcaccaggca aggaggcccc ggagccttag cagggacttg
     4981 cccaacggca cagctgattg gtgctcgact cccagcatgc atattttgtt gtaaaaaccc
     5041 aggtttgtaa ttcacatcca caaccctaag ccttttaaag cggaggcaaa cgttaacaaa
     5101 ccccatcgtg gtccatggag cctttcaaaa tgggtgccct ggaggggcct gacagtgggt
     5161 tccttccatg gcggatactc ccttgaaatt tcacattcca tcaaagttta aagcatttac
     5221 ttcagaataa gttatttatg tgtatatgtt caataaatat agtttttaat ttatatctgt
     5281 acatattaga cacacgcgag ccatcttgcc agaattctga cagtgatgtc attttattcc
     5341 cctcccaaaa catgccccag gaacaagcac cccttgcctc tgtggcagct gcgagtgtgt
     5401 ctgatttcat aacgaagcca ggctgagggc aagaggcccg acaggcaggc cagggtcccc
     5461 ggagaagccg gatttcttcg caatgtcact ggtacttagc agatggtcgt gttcacaggg
     5521 gctagccacc tgtactacgg tgccgaccgc aatgcgagga atgtggtggc cacgcgatgg
     5581 agctggccgg aggacaggtc aggtgagggg gccgacccct gcggggctca gggctccttc
     5641 cattctaacc ttgagccaca gtgtcactct tcagggctct gctcctggct ctattttgtt
     5701 aaggtgtcat cagccttcaa cctccattta tatattttta taatgttaag ccactgacca
     5761 actttttcat agaaacaact atcagattcg agggctccct ttgtccccct gacctgggca
     5821 caggcatggg cgatggctcc ctcttcccag aatctgcggg gaactggggc aaggcccatc
     5881 tgtctgcaag gctggtagct ctctccctcg gcttccagag tccacggcag ttgcaaggta
     5941 accagtgtgg atctgctgag tcaccccagg caccagactt accccaggag catgggccac
     6001 tgtccatggg gccttccttg agaccttccc ttggggctgg cgggcggcgg cttaccagct
     6061 gggtcagtcc catctcgccc ctctgtgcct atgccctggc gttgccaggc ttaaccttcc
     6121 cctgtatctt actcatccca gaattattac tggccatttc taaatagaca aaaactaccc
     6181 taaaatgtgg agatttactc cttttgaaaa gtctgtttca aaaagtgtgg cctcagctct
     6241 ggaggccacc cccaggaagg agtttgaggt tcattgtcat tatggccaca gtttggtgct
     6301 tgtgcacggg actcaaaggg cagcgtcgaa gctggggtta ggtgtagggt tgtgagcaaa
     6361 gctcccctgt ttgtggccac accttccctt tccaaaaagg ctattaattc tactcaccca
     6421 ctatttgggg ggatgtaaat tttgcccaca gactttgcca cccaggtgag gtgctgtgtt
     6481 catgcacgga actgatgtca ttagacacca tccaaggatg ggcagcctgc agtgtggccg
     6541 gcgactgcca tccagagaaa aggcaccttg tgccacaggg cggggccgac accctgagct
     6601 cacaggtggc ccctgcattc aggtggatgt ccatccttga aacagcctct tctgggaaca
     6661 cagcactgga ggtctccagg catcagggtt ggccggcagg gtgccctgca gcttacataa
     6721 aggtcgtggc aatgtttcaa tgttaaacta aactattgcc ttcctcttac ttttcctaaa
     6781 atgattttct aatgggaaca gtcacttcaa gttcatgaca tagtttgaag gaggataatt
     6841 cattttttcc tggttgtcac tgtcttctta tccccttggt gggctgggtt catactgtga
     6901 aataaactaa aatgaaacca aaacgttcct gccgtaaata cgtggcacag cactgcagaa
     6961 aaagcagtca cctctattcg tgagtcccca agccatagca gggcgtctgt aaaagaaggg
     7021 aaactgcacc caggaagtgg tgggcgtcag acgcatcctt tcgcccgtag ttcatgaaat
     7081 agttccggct ttggtcttcc agaaaatctc aacttcaccg tgcacaagtg ttaccgggtc
     7141 tttttagtgt agccatggaa gatactttca aaaggacatc ttcagatcat aagtcttagg
     7201 accaaaggtg tttttaatga gataatcttt ttgattttct aacaagaaag gaaatattca
     7261 ctttacattt taacatcaac ttttaagtga tattcagaat taattgacca tggtgatacg
     7321 ttgtaaaatg ttttcatatt aagaatgtaa ttatttcctt attgtatttt ttaaaagcta
     7381 aagtcatatt taaaattctc tttgaaagaa acaggcaaag aaacagcaaa gattttttta
     7441 aattaaatat gttagtagtg cccacttcaa atgactgtaa atatattttc actgtttctc
     7501 aatgtattta attcataaag taaaaatttt atgaaaagaa acttgcaagg aaaatcctag
     7561 ttttcagtcc actcgcccca ctttgtagta gcttcactga atttcctgct ttcctggttg
     7621 aattttctac tgtttcacgt ggtgtaaccc tagactggcg cccttggcgt gcccctttgt
     7681 gtatgaataa acttctctcc ccca
//
