LOCUS       NM_022767               3072 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens apoptosis enhancing nuclease (AEN), mRNA.
ACCESSION   NM_022767
VERSION     NM_022767.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3072)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3072)
  AUTHORS   Chen,S., Fragoza,R., Klei,L., Liu,Y., Wang,J., Roeder,K., Devlin,B.
            and Yu,H.
  TITLE     An interactome perturbation framework prioritizes damaging missense
            mutations for developmental disorders
  JOURNAL   Nat Genet 50 (7), 1032-1040 (2018)
   PUBMED   29892012
REFERENCE   5  (bases 1 to 3072)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 3072)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 3072)
  AUTHORS   Eby,K.G., Rosenbluth,J.M., Mays,D.J., Marshall,C.B., Barton,C.E.,
            Sinha,S., Johnson,K.N., Tang,L. and Pietenpol,J.A.
  TITLE     ISG20L1 is a p53 family target gene that modulates genotoxic
            stress-induced autophagy
  JOURNAL   Mol Cancer 9, 95 (2010)
   PUBMED   20429933
  REMARK    GeneRIF: identification of ISG20L1 as a p53 family target and
            discovery that modulation of this target can regulate autophagic
            processes further strengthens the connection between p53 signaling
            and autophagy
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3072)
  AUTHORS   Kawase,T., Ichikawa,H., Ohta,T., Nozaki,N., Tashiro,F., Ohki,R. and
            Taya,Y.
  TITLE     p53 target gene AEN is a nuclear exonuclease required for
            p53-dependent apoptosis
  JOURNAL   Oncogene 27 (27), 3797-3810 (2008)
   PUBMED   18264133
  REMARK    GeneRIF: AEN is an important downstream mediator of p53 in
            apoptosis induction in cancer.
REFERENCE   9  (bases 1 to 3072)
  AUTHORS   Lee,J.H., Koh,Y.A., Cho,C.K., Lee,S.J., Lee,Y.S. and Bae,S.
  TITLE     Identification of a novel ionizing radiation-induced nuclease, AEN,
            and its functional characterization in apoptosis
  JOURNAL   Biochem Biophys Res Commun 337 (1), 39-47 (2005)
   PUBMED   16171785
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC013489.17.
            
            On Nov 23, 2018 this sequence version replaced NM_022767.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.198050.1,
                                           SRR1803616.258846.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000332810.4/ ENSP00000331944.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC013489.17        12280-12322         c
            44-647              AC013489.17        6913-7516           c
            648-848             AC013489.17        4236-4436           c
            849-3072            AC013489.17        1381-3604           c
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..3072
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /note="apoptosis enhancing nuclease"
                     /db_xref="GeneID:64782"
                     /db_xref="HGNC:HGNC:25722"
                     /db_xref="MIM:610177"
     exon            1..43
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /inference="alignment:Splign:2.1.0"
     exon            44..647
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..1085
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /note="interferon stimulated exonuclease gene 20kDa-like
                     1; interferon-stimulated 20 kDa exonuclease-like 1"
                     /codon_start=1
                     /product="apoptosis-enhancing nuclease"
                     /protein_id="NP_073604.3"
                     /db_xref="CCDS:CCDS10344.1"
                     /db_xref="GeneID:64782"
                     /db_xref="HGNC:HGNC:25722"
                     /db_xref="MIM:610177"
                     /translation="MVPREAPESAQCLCPSLTIPNAKDVLRKRHKRRSRQHQRFMARK
                     ALLQEQGLLSMPPEPGSSPLPTPFGAATATEAASSGKQCLRAGSGSAPCSRRPAPGKA
                     SGPLPSKCVAIDCEMVGTGPRGRVSELARCSIVSYHGNVLYDKYIRPEMPIADYRTRW
                     SGITRQHMRKAVPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQTRDTTYVP
                     NFLSEPGLHTRARVSLKDLALQLLHKKIQVGQHGHSSVEDATTAMELYRLVEVQWEQQ
                     EARSLWTCPEDREPDSSTDMEQYMEDQYWPDDLAHGSRGGAREAQDRRN"
     exon            648..848
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /inference="alignment:Splign:2.1.0"
     exon            849..3072
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3050..3055
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="AEN"
                     /gene_synonym="ISG20L1; pp12744"
                     /note="major polyA site"
ORIGIN      
        1 ccggacgcca cgtgcagacc ggaagagaca cgcggggctt caggctgctg ccccattgga
       61 agattactcc ccaggcttcc cttgccccaa gcagtgagct gactggaatg gtaccccggg
      121 aggcccctga gtctgctcag tgcctgtgcc cttccctcac catcccaaat gccaaggatg
      181 tgcttcggaa gaggcacaag agaaggagcc gacagcacca gcggttcatg gcccggaagg
      241 ccttgctgca ggagcagggg ctgctgagca tgcctccaga accagggtcc tccccactgc
      301 ccaccccttt cggggcagcg acagcaactg aagctgccag cagtgggaag cagtgtctga
      361 gggctggatc tggcagtgcc ccatgcagca gaaggcctgc tcccgggaaa gcctcagggc
      421 ccttgcccag caagtgtgtg gctatcgact gtgagatggt gggcacggga ccccgagggc
      481 gggtaagcga gctggcccgc tgttccattg tgagctacca tggcaatgtc ctctatgaca
      541 agtacatcag gcctgagatg cccatcgctg actaccgtac ccgctggagt ggcatcactc
      601 ggcagcacat gcgcaaggct gtccccttcc aggtggccca gaaagagatc cttaagctcc
      661 tgaagggcaa ggtggtggtg gggcacgcgc tgcacaacga cttccaggcg ctcaagtatg
      721 tccaccctcg gagccagacc cgggatacga cctatgtccc aaacttcctc agcgagcccg
      781 gcctccacac ccgggcccgg gtctctctaa aggacctggc cctgcagctg ctgcacaaga
      841 agatccaggt gggccagcac gggcactcat cagtagaaga tgccacgaca gccatggagc
      901 tctaccggct ggtggaggtg cagtgggaac agcaggaggc ccgcagcctc tggacctgcc
      961 ccgaggacag agaacctgac agcagcacag acatggaaca gtacatggag gaccagtact
     1021 ggcccgatga cctggcccac ggcagcagag gaggagccag ggaggcacag gacagaagga
     1081 attgagaagg gggcggggct ccctggctgg gcttccggtg tggccggtag gaagtggggg
     1141 ccaggagagc agcgggcact ccttcctggg cagggtgggg caggatgcag tgagccagcc
     1201 ccagggccag aggagtaggg gtcatctgtt accttgacac cctctgcaca cagcatagcc
     1261 ctctctctct ccagggctgt tggttctttc ttctgactcc tgtggttttg ctaatggcac
     1321 tttacagact ccatggagat gtcaggtgga ccatcttcta gggcccagca ggagtaggga
     1381 atgtgccaac agactgccca ggttgccgtg gccttcccca ccccccagat ctcctgagtc
     1441 atcatgctgt gctaatgaaa gggatcatat catcctctct ggggatggtg ggtgggggtg
     1501 tcaatatcct ggagctccct taccccaact caatgacttg ggggtaaagt tctcttcctt
     1561 ttgttgccta cctcttcctc cactcatttg ggttcagaat aaacatgtcc tgaagttaaa
     1621 gaggagttaa gtcctaaagg aggcatttct tccccacctc cctgacctgg aactctggct
     1681 acagccattg taggaactcc gtgcctggcc tgtcagctcc ctgctaggct acagtggaat
     1741 agcagagccc acaggcttct cgtggggagt tggctcctta acatttcttg gcaacagaaa
     1801 gccccaggca cagctcaggg aggagggaag gcaggtaagc tttggacgag aactggcata
     1861 tttattttga cccaaatcag ggatttcccc agtccaccca gtactgggct cttaagcaaa
     1921 agtctgagaa acaagacagt ggtttgaatt ctggggcctt tgtgtaggat tgtgcctgac
     1981 ctttatttat ttattaacag cagggccact cgtctagggc agtggagtct gcgtgtctcc
     2041 tggggctggg gcagggcatt ggcagttacg cagtggccct gaacctggtc tggtgccccc
     2101 gaacctggtc tgatgccccc tcagctcttt gacaatcact gtggctgttg ggttttctcc
     2161 tatttctaaa aatgttctct tctttcctaa gtgacagttt tgaagtattg gataaccaag
     2221 agctcaggtc acacagacct tggagcccca tctttgcttg cagcttagct ttgagatact
     2281 aagcaagcga gggacttcac tcttgaggct gttttttcct catctgtaaa gtggggatag
     2341 tggtacggcc tacctcatag ggttgtaatg agcactaaat gtgaagtgct tggcacagtg
     2401 cctggcacat ggtgagccac agctactgtg agtttctgtt tatccccctt tttttttttt
     2461 aagcccattg tttattcttt gagaatttgt tgtaacttct tcaggataac acctgagtcc
     2521 acaggctgag cagctgtggc ccagacagaa ctgctccggc ttggctgttc cagcaggtgg
     2581 ggcgctggcc tcggtgaggg cacagcagca aggttcacgg atatccgtgt gtcttgtctg
     2641 tggccaccag gcacaggttt ggcttccggt cagtgtcccg acactgtgcg ggaggtgaca
     2701 acagagcaaa gcagcgcagg ggtcagggag gtacagacac tgctgaaatc acactacccc
     2761 accctcagct gaagccccac gttccacaaa cttggggtca tagattgtcc agtcactggc
     2821 tccctccctg tcagcacagc acagaggaag gggctaactg aatcttttac cacttctggc
     2881 ctggctccag aactttgttc tagattcctt aaaagtcggt agctgatgtc aaactcaatt
     2941 gagcagtagc tttgatccct tggtctgggg gtcgaaggaa gatggtgctg ttatcagcgg
     3001 ggaaatgtac tatttaagat cagctttgtt gtaaaaccat ttgttctaga ataaaactca
     3061 attggaaacg tg
//
