LOCUS       NM_000675               2529 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens adenosine A2a receptor (ADORA2A), transcript variant
            3, mRNA.
ACCESSION   NM_000675
VERSION     NM_000675.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Lindo,J., Nogueira,C., Soares,R., Cunha,N., Almeida,M.R.,
            Rodrigues,L., Coelho,P., Rodrigues,F., Cunha,R.A. and Goncalves,T.
  TITLE     Genetic Polymorphisms of P2RX7 but Not of ADORA2A Are Associated
            with the Severity of SARS-CoV-2 Infection
  JOURNAL   Int J Mol Sci 25 (11), 6135 (2024)
   PUBMED   38892324
  REMARK    GeneRIF: Genetic Polymorphisms of P2RX7 but Not of ADORA2A Are
            Associated with the Severity of SARS-CoV-2 Infection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Chen,H., Xing,G., Xu,W., Chen,Y., Xia,L., Huang,H., Huang,J.,
            Hong,Q., Luo,T., Wang,H. and Wu,Q.
  TITLE     The adenosine A2A receptor in human sperm: its role in sperm
            motility and association with in vitro fertilization outcomes
  JOURNAL   Front Endocrinol (Lausanne) 15, 1410370 (2024)
   PUBMED   38872963
  REMARK    GeneRIF: The adenosine A2A receptor in human sperm: its role in
            sperm motility and association with in vitro fertilization
            outcomes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2529)
  AUTHORS   Lebon,G., Warne,T., Edwards,P.C., Bennett,K., Langmead,C.J.,
            Leslie,A.G. and Tate,C.G.
  TITLE     Agonist-bound adenosine A2A receptor structures reveal common
            features of GPCR activation
  JOURNAL   Nature 474 (7352), 521-525 (2011)
   PUBMED   21593763
  REMARK    GeneRIF: crystal structures of the thermostabilized human adenosine
            A(2A) receptor (A(2A)R-GL31) bound to its endogenous agonist
            adenosine and the synthetic agonist NECA
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2529)
  AUTHORS   Xu,F., Wu,H., Katritch,V., Han,G.W., Jacobson,K.A., Gao,Z.G.,
            Cherezov,V. and Stevens,R.C.
  TITLE     Structure of an agonist-bound human A2A adenosine receptor
  JOURNAL   Science 332 (6027), 322-327 (2011)
   PUBMED   21393508
  REMARK    GeneRIF: study reports the crystal structure of the A(2A) adenosine
            receptor bound to an agonist UK-432097 at 2.7 angstrom resolution
REFERENCE   5  (bases 1 to 2529)
  AUTHORS   Jaakola,V.P., Griffith,M.T., Hanson,M.A., Cherezov,V., Chien,E.Y.,
            Lane,J.R., Ijzerman,A.P. and Stevens,R.C.
  TITLE     The 2.6 angstrom crystal structure of a human A2A adenosine
            receptor bound to an antagonist
  JOURNAL   Science 322 (5905), 1211-1217 (2008)
   PUBMED   18832607
REFERENCE   6  (bases 1 to 2529)
  AUTHORS   Le,F., Townsend-Nicholson,A., Baker,E., Sutherland,G.R. and
            Schofield,P.R.
  TITLE     Characterization and chromosomal localization of the human A2a
            adenosine receptor gene: ADORA2A
  JOURNAL   Biochem Biophys Res Commun 223 (2), 461-467 (1996)
   PUBMED   8670304
REFERENCE   7  (bases 1 to 2529)
  AUTHORS   MacCollin,M., Peterfreund,R., MacDonald,M., Fink,J.S. and
            Gusella,J.
  TITLE     Mapping of a human A2a adenosine receptor (ADORA2) to chromosome 22
  JOURNAL   Genomics 20 (2), 332-333 (1994)
   PUBMED   8020991
REFERENCE   8  (bases 1 to 2529)
  AUTHORS   Makujina,S.R., Sabouni,M.H., Bhatia,S., Douglas,F.L. and
            Mustafa,S.J.
  TITLE     Vasodilatory effects of adenosine A2 receptor agonists CGS 21680
            and CGS 22492 in human vasculature
  JOURNAL   Eur J Pharmacol 221 (2-3), 243-247 (1992)
   PUBMED   1426003
REFERENCE   9  (bases 1 to 2529)
  AUTHORS   Furlong,T.J., Pierce,K.D., Selbie,L.A. and Shine,J.
  TITLE     Molecular characterization of a human brain adenosine A2 receptor
  JOURNAL   Brain Res Mol Brain Res 15 (1-2), 62-66 (1992)
   PUBMED   1331670
REFERENCE   10 (bases 1 to 2529)
  AUTHORS   Libert,F., Passage,E., Parmentier,M., Simons,M.J., Vassart,G. and
            Mattei,M.G.
  TITLE     Chromosomal mapping of A1 and A2 adenosine receptors, VIP receptor,
            and a new subtype of serotonin receptor
  JOURNAL   Genomics 11 (1), 225-227 (1991)
   PUBMED   1662665
  REMARK    Erratum:[Genomics 1994 Sep 1;23(1):305]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA942067.1, BC013780.1,
            AK123058.1 and CA307692.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000675.5.
            
            Summary: This gene encodes a member of the guanine
            nucleotide-binding protein (G protein)-coupled receptor (GPCR)
            superfamily, which is subdivided into classes and subtypes. The
            receptors are seven-pass transmembrane proteins that respond to
            extracellular cues and activate intracellular signal transduction
            pathways. This protein, an adenosine receptor of A2A subtype, uses
            adenosine as the preferred endogenous agonist and preferentially
            interacts with the G(s) and G(olf) family of G proteins to increase
            intracellular cAMP levels. It plays an important role in many
            biological functions, such as cardiac rhythm and circulation,
            cerebral and renal blood flow, immune function, pain regulation,
            and sleep. It has been implicated in pathophysiological conditions
            such as inflammatory diseases and neurodegenerative disorders.
            Alternative splicing results in multiple transcript variants. A
            read-through transcript composed of the upstream SPECC1L (sperm
            antigen with calponin homology and coiled-coil domains 1-like) and
            ADORA2A (adenosine A2a receptor) gene sequence has been identified,
            but it is thought to be non-coding. [provided by RefSeq, Jun 2013].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. Variants, 1, 2, 3, 4, and 5 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013780.1, SRR1163658.166108.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000337539.12/ ENSP00000336630.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DA942067.1         38-73
            37-1504             BC013780.1         1-1468
            1505-2513           AK123058.1         1452-2460
            2514-2529           CA307692.1         18-33               c
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.23"
     gene            1..2529
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /note="adenosine A2a receptor"
                     /db_xref="GeneID:135"
                     /db_xref="HGNC:HGNC:263"
                     /db_xref="MIM:102776"
     exon            1..148
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /inference="alignment:Splign:2.1.0"
     exon            149..754
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /inference="alignment:Splign:2.1.0"
     CDS             423..1661
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /note="adenosine receptor subtype A2a"
                     /codon_start=1
                     /product="adenosine receptor A2a"
                     /protein_id="NP_000666.2"
                     /db_xref="CCDS:CCDS13826.1"
                     /db_xref="GeneID:135"
                     /db_xref="HGNC:HGNC:263"
                     /db_xref="MIM:102776"
                     /translation="MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYF
                     VVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDR
                     YIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCG
                     EGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPG
                     ERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVL
                     SHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQV
                     SLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKG
                     VCPEPPGLDDPLAQDGAGVS"
     exon            755..2529
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2488..2493
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /note="hexamer: AATAAA"
     polyA_site      2529
                     /gene="ADORA2A"
                     /gene_synonym="A2aR; ADORA2; RDC8"
                     /note="major polyA site"
ORIGIN      
        1 gaagctctgc ctgggcctca gggactgtga catggagcag gagccgcccc cagccaagct
       61 gctttcagca cagcgtgggc ccccagcacc ttggtgcggg gtgcggcccc tcggaggagg
      121 gctgtcaggt gaagcctcgt gtgagggggt gcctcaggaa ccctgaagct gggctgagcc
      181 atgatgctgc tgccagaacc cctgcagagg gcctggtttc aggagactca gagtcctctg
      241 tgaaaaagcc cttggagagc gccccagcag ggctgcactt ggctcctgtg aggaaggggc
      301 tcaggggtct gggcccctcc gcctgggccg ggctgggagc caggcgggcg gctgggctgc
      361 agcaatggac cgtgagctgg cccagcccgc gtccgtgctg agcctgcctg tcgtctgtgg
      421 ccatgcccat catgggctcc tcggtgtaca tcacggtgga gctggccatt gctgtgctgg
      481 ccatcctggg caatgtgctg gtgtgctggg ccgtgtggct caacagcaac ctgcagaacg
      541 tcaccaacta ctttgtggtg tcactggcgg cggccgacat cgcagtgggt gtgctcgcca
      601 tcccctttgc catcaccatc agcaccgggt tctgcgctgc ctgccacggc tgcctcttca
      661 ttgcctgctt cgtcctggtc ctcacgcaga gctccatctt cagtctcctg gccatcgcca
      721 ttgaccgcta cattgccatc cgcatcccgc tccggtacaa tggcttggtg accggcacga
      781 gggctaaggg catcattgcc atctgctggg tgctgtcgtt tgccatcggc ctgactccca
      841 tgctaggttg gaacaactgc ggtcagccaa aggagggcaa gaaccactcc cagggctgcg
      901 gggagggcca agtggcctgt ctctttgagg atgtggtccc catgaactac atggtgtact
      961 tcaacttctt tgcctgtgtg ctggtgcccc tgctgctcat gctgggtgtc tatttgcgga
     1021 tcttcctggc ggcgcgacga cagctgaagc agatggagag ccagcctctg ccgggggagc
     1081 gggcacggtc cacactgcag aaggaggtcc atgctgccaa gtcactggcc atcattgtgg
     1141 ggctctttgc cctctgctgg ctgcccctac acatcatcaa ctgcttcact ttcttctgcc
     1201 ccgactgcag ccacgcccct ctctggctca tgtacctggc catcgtcctc tcccacacca
     1261 attcggttgt gaatcccttc atctacgcct accgtatccg cgagttccgc cagaccttcc
     1321 gcaagatcat tcgcagccac gtcctgaggc agcaagaacc tttcaaggca gctggcacca
     1381 gtgcccgggt cttggcagct catggcagtg acggagagca ggtcagcctc cgtctcaacg
     1441 gccacccgcc aggagtgtgg gccaacggca gtgctcccca ccctgagcgg aggcccaatg
     1501 gctatgccct ggggctggtg agtggaggga gtgcccaaga gtcccagggg aacacgggcc
     1561 tcccagacgt ggagctcctt agccatgagc tcaagggagt gtgcccagag ccccctggcc
     1621 tagatgaccc cctggcccag gatggagcag gagtgtcctg atgattcatg gagtttgccc
     1681 cttcctaagg gaaggagatc tttatctttc tggttggctt gaccagtcac gttgggagaa
     1741 gagagagagt gccaggagac cctgagggca gccggttcct actttggact gagagaaggg
     1801 agccccaggc tggagcagca tgaggcccag caagaagggc ttgggttctg aggaagcaga
     1861 tgtttcatgc tgtgaggcct tgcaccaggt gggggccaca gcaccagcag catctttgct
     1921 gggcagggcc cagccctcca ctgcagaagc atctggaagc accaccttgt ctccacagag
     1981 cagcttgggc acagcagact ggcctggccc tgagactggg gagtggctcc aacagcctcc
     2041 tgccacccac acaccactct ccctagactc tcctagggtt caggagctgc tgggcccaga
     2101 ggtgacattt gacttttttc caggaaaaat gtaagtgtga ggaaaccctt tttattttat
     2161 tacctttcac tctctggctg ctgggtctgc cgtcggtcct gctgctaacc tggcaccaga
     2221 gcctctgccc ggggagcctc aggcagtcct ctcctgctgt cacagctgcc atccacttct
     2281 cagtcccagg gccatctctt ggagtgacaa agctgggatc aaggacaggg agttgtaaca
     2341 gagcagtgcc agagcatggg cccaggtccc aggggagagg ttggggctgg caggccactg
     2401 gcatgtgctg agtagcgcag agctacccag tgagaggcct tgtctaactg cctttccttc
     2461 taaagggaat gtttttttct gagataaaat aaaaacgagc cacatcgtgt tttaagcttg
     2521 tccaaatga
//
