LOCUS       NM_001258429            1252 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens adipose secreted signaling protein (ADISSP),
            transcript variant 2, mRNA.
ACCESSION   NM_001258429
VERSION     NM_001258429.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Chen,Q., Huang,L., Pan,D., Hu,K., Li,R., Friedline,R.H., Kim,J.K.,
            Zhu,L.J., Guertin,D.A. and Wang,Y.X.
  TITLE     A brown fat-enriched adipokine Adissp controls adipose
            thermogenesis and glucose homeostasis
  JOURNAL   Nat Commun 13 (1), 7633 (2022)
   PUBMED   36496438
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Gao,J., Wang,Y., Zhang,W., Zhang,J., Lu,S., Meng,K., Yin,X., Sun,Z.
            and He,Q.Y.
  TITLE     C20orf27 Promotes Cell Growth and Proliferation of Colorectal
            Cancer via the TGFbetaR-TAK1-NFkB Pathway
  JOURNAL   Cancers (Basel) 12 (2), 336 (2020)
   PUBMED   32024300
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1252)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   4  (bases 1 to 1252)
  AUTHORS   Boldt,K., van Reeuwijk,J., Lu,Q., Koutroumpas,K., Nguyen,T.M.,
            Texier,Y., van Beersum,S.E., Horn,N., Willer,J.R., Mans,D.A.,
            Dougherty,G., Lamers,I.J., Coene,K.L., Arts,H.H., Betts,M.J.,
            Beyer,T., Bolat,E., Gloeckner,C.J., Haidari,K., Hetterschijt,L.,
            Iaconis,D., Jenkins,D., Klose,F., Knapp,B., Latour,B.,
            Letteboer,S.J., Marcelis,C.L., Mitic,D., Morleo,M., Oud,M.M.,
            Riemersma,M., Rix,S., Terhal,P.A., Toedt,G., van Dam,T.J., de
            Vrieze,E., Wissinger,Y., Wu,K.M., Apic,G., Beales,P.L.,
            Blacque,O.E., Gibson,T.J., Huynen,M.A., Katsanis,N., Kremer,H.,
            Omran,H., van Wijk,E., Wolfrum,U., Kepes,F., Davis,E.E., Franco,B.,
            Giles,R.H., Ueffing,M., Russell,R.B. and Roepman,R.
  CONSRTM   UK10K Rare Diseases Group
  TITLE     An organelle-specific protein landscape identifies novel diseases
            and molecular mechanisms
  JOURNAL   Nat Commun 7, 11491 (2016)
   PUBMED   27173435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1252)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BF978883.1, AK024220.1 and DB317576.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001258429.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the coding region, but maintains the reading frame, compared to
            variant 1. Variants 2 and 3 encode the same isoform (2), which is
            shorter than isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.33782.1, BC012196.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142680
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379772.4/ ENSP00000369097.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BF978883.1         54-62
            10-1250             AK024220.1         1-1241
            1251-1252           DB317576.1         10-11               c
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..1252
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /note="adipose secreted signaling protein"
                     /db_xref="GeneID:54976"
                     /db_xref="HGNC:HGNC:15873"
                     /db_xref="MIM:620373"
     exon            1..91
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     exon            92..196
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..702
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /note="isoform 2 is encoded by transcript variant 2;
                     UPF0687 protein C20orf27"
                     /codon_start=1
                     /product="adipose-secreted signaling protein isoform 2"
                     /protein_id="NP_001245358.1"
                     /db_xref="CCDS:CCDS58763.1"
                     /db_xref="GeneID:54976"
                     /db_xref="HGNC:HGNC:15873"
                     /db_xref="MIM:620373"
                     /translation="MAAANKGNKPRVRSIRFAAGHDAEGSHSHVHFDEKLHDSVVMVT
                     QESDSSFLVKVGFLKILHRYEITFTLPPVHRLSKDVREAPVPSLHLKLLSVVPVPEGY
                     SVKCEYSAHKEGVLKEEILLACEGGTGTCVRVTVQARVMDRHHGTPMLLDGVKCVGAE
                     LEYDSEHSDWHGFD"
     exon            197..339
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     exon            340..478
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     exon            479..601
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     exon            602..1252
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1229..1234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /note="hexamer: AATAAA"
     polyA_site      1252
                     /gene="ADISSP"
                     /gene_synonym="C20orf27"
                     /note="major polyA site"
ORIGIN      
        1 cccttcctcg tgctcggcgt tgagctcctg cagccgccgc cgctgcagtg gtcgtccctg
       61 ccctccccgg ccccggggtg caccccgcaa ggctcccgct ggtgtccctg gagcatggga
      121 ggctgctgag cgtgagtggc ggtgtctggc aggagctgcg tggcagggag ggcgtccatg
      181 gctgcagcca acaagggcaa caagcccaga gtccggagta tccgctttgc ggcaggccac
      241 gatgcagaag gatcccacag ccacgtccac tttgatgaga agctgcatga ctcggtggtc
      301 atggtcaccc aggagagtga cagcagcttt ctggtcaagg ttggcttcct gaagatcctg
      361 cacaggtatg agattacctt cactctgccc ccagtgcaca ggctgagcaa ggatgtccgc
      421 gaggcacctg tccccagcct gcacctcaag ctcctcagcg tggtgcccgt ccctgaaggt
      481 tatagtgtca agtgtgagta ctcggcgcac aaagagggcg tcctcaaaga ggagatactg
      541 ctagcctgcg aaggtggcac tggcacctgt gtgcgcgtga cggtgcaggc ccgcgtcatg
      601 gaccggcacc acggcacgcc catgctgctg gatggtgtca agtgtgtggg cgccgagctg
      661 gaatacgact cagagcacag cgactggcac ggctttgact gaggcccgag gccccgcctg
      721 ccccgggccc ctcagcctta aaccccgcct tgtccccccg acatgctgcg tgatggtgtg
      781 gcttcctcgc ccctctctgg ggtgggtgtg ggggtggagt ggccttgccc acgcctctca
      841 cctctgcctt catttgtgct gccaccctgc ccctccctcg tcctcctctc ccacttcctc
      901 ctctctgtgt gcctcagtct cctgccggaa gaaatgggtt gagcccgaaa ggaggctgtc
      961 tgaggaaggg agagggaggg cctggggtgg gtcccccact ccccacccca agccacaggg
     1021 actcccacca gggtctggga gaggacggag ctggctctgt ggcgtcgtgg ccccattact
     1081 gctgccttgc ttcagccacc tctcctgccc ctccctagtc cccactgctg tccaccatga
     1141 gtaggaggga ggtgcagtcc ccagccccca cccctcaggt ctgtgttact tggtttttaa
     1201 gcgactggtt gggatagaac cctaaagaaa taaacttcca gtggataccg ga
//
