LOCUS       NM_078481               3190 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens adhesion G protein-coupled receptor E5 (ADGRE5),
            transcript variant 1, mRNA.
ACCESSION   NM_078481
VERSION     NM_078481.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Mao,C., Zhao,R.J., Dong,Y.J., Gao,M., Chen,L.N., Zhang,C., Xiao,P.,
            Guo,J., Qin,J., Shen,D.D., Ji,S.Y., Zang,S.K., Zhang,H., Wang,W.W.,
            Shen,Q., Sun,J.P. and Zhang,Y.
  TITLE     Conformational transitions and activation of the adhesion receptor
            CD97
  JOURNAL   Mol Cell 84 (3), 570-583 (2024)
   PUBMED   38215752
  REMARK    GeneRIF: Conformational transitions and activation of the adhesion
            receptor CD97.
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Ravn-Boess,N., Roy,N., Hattori,T., Bready,D., Donaldson,H.,
            Lawson,C., Lapierre,C., Korman,A., Rodrick,T., Liu,E.,
            Frenster,J.D., Stephan,G., Wilcox,J., Corrado,A.D., Cai,J.,
            Ronnen,R., Wang,S., Haddock,S., Sabio Ortiz,J., Mishkit,O.,
            Khodadadi-Jamayran,A., Tsirigos,A., Fenyo,D., Zagzag,D., Drube,J.,
            Hoffmann,C., Perna,F., Jones,D.R., Possemato,R., Koide,A.,
            Koide,S., Park,C.Y. and Placantonakis,D.G.
  TITLE     The expression profile and tumorigenic mechanisms of CD97 (ADGRE5)
            in glioblastoma render it a targetable vulnerability
  JOURNAL   Cell Rep 42 (11), 113374 (2023)
   PUBMED   37938973
  REMARK    GeneRIF: The expression profile and tumorigenic mechanisms of CD97
            (ADGRE5) in glioblastoma render it a targetable vulnerability.
REFERENCE   3  (bases 1 to 3190)
  AUTHORS   Wang,N., Qian,Y., Xia,R., Zhu,X., Xiong,Y., Zhang,A., Guo,C. and
            He,Y.
  TITLE     Structural basis of CD97 activation and G-protein coupling
  JOURNAL   Cell Chem Biol 30 (11), 1343-1353 (2023)
   PUBMED   37673067
  REMARK    GeneRIF: Structural basis of CD97 activation and G-protein
            coupling.
REFERENCE   4  (bases 1 to 3190)
  AUTHORS   Meng,Z.W., Zhang,L., Cai,X.R., Wang,X., She,F.F. and Chen,Y.L.
  TITLE     IL-8 is a novel prometastatic chemokine in intrahepatic
            cholangiocarcinoma that induces CXCR2-PI3K/AKT signaling upon CD97
            activation
  JOURNAL   Sci Rep 13 (1), 18711 (2023)
   PUBMED   37907543
  REMARK    GeneRIF: IL-8 is a novel prometastatic chemokine in intrahepatic
            cholangiocarcinoma that induces CXCR2-PI3K/AKT signaling upon CD97
            activation.
            Erratum:[Sci Rep. 2024 Apr 11;14(1):8478. doi:
            10.1038/s41598-024-58952-5.. PMID: 38605065]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3190)
  AUTHORS   Hsiao,C.C., Chen,H.Y., Chang,G.W. and Lin,H.H.
  TITLE     GPS autoproteolysis is required for CD97 to up-regulate the
            expression of N-cadherin that promotes homotypic cell-cell
            aggregation
  JOURNAL   FEBS Lett 585 (2), 313-318 (2011)
   PUBMED   21156175
  REMARK    GeneRIF: expression of the wild type - but not the GPS
            cleavage-deficient CD97 up-regulates the expression of N-cadherin,
            leading to Ca(++)-dependent cell-cell aggregation.
REFERENCE   6  (bases 1 to 3190)
  AUTHORS   Aust,G., Eichler,W., Laue,S., Lehmann,I., Heldin,N.E., Lotz,O.,
            Scherbaum,W.A., Dralle,H. and Hoang-Vu,C.
  TITLE     CD97: a dedifferentiation marker in human thyroid carcinomas
  JOURNAL   Cancer Res 57 (9), 1798-1806 (1997)
   PUBMED   9135025
REFERENCE   7  (bases 1 to 3190)
  AUTHORS   Gray,J.X., Haino,M., Roth,M.J., Maguire,J.E., Jensen,P.N.,
            Yarme,A., Stetler-Stevenson,M.A., Siebenlist,U. and Kelly,K.
  TITLE     CD97 is a processed, seven-transmembrane, heterodimeric receptor
            associated with inflammation
  JOURNAL   J Immunol 157 (12), 5438-5447 (1996)
   PUBMED   8955192
REFERENCE   8  (bases 1 to 3190)
  AUTHORS   Hamann,J., Vogel,B., van Schijndel,G.M. and van Lier,R.A.
  TITLE     The seven-span transmembrane receptor CD97 has a cellular ligand
            (CD55, DAF)
  JOURNAL   J Exp Med 184 (3), 1185-1189 (1996)
   PUBMED   9064337
REFERENCE   9  (bases 1 to 3190)
  AUTHORS   Hamann,J., Hartmann,E. and van Lier,R.A.
  TITLE     Structure of the human CD97 gene: exon shuffling has generated a
            new type of seven-span transmembrane molecule related to the
            secretin receptor superfamily
  JOURNAL   Genomics 32 (1), 144-147 (1996)
   PUBMED   8786105
REFERENCE   10 (bases 1 to 3190)
  AUTHORS   Hamann,J., Eichler,W., Hamann,D., Kerstens,H.M., Poddighe,P.J.,
            Hoovers,J.M., Hartmann,E., Strauss,M. and van Lier,R.A.
  TITLE     Expression cloning and chromosomal mapping of the leukocyte
            activation antigen CD97, a new seven-span transmembrane molecule of
            the secretion receptor superfamily with an unusual extracellular
            domain
  JOURNAL   J Immunol 155 (4), 1942-1950 (1995)
   PUBMED   7636245
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK314697.1, U76764.1 and
            AI890331.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_078481.3.
            
            Summary: This gene encodes a member of the EGF-TM7 subfamily of
            adhesion G protein-coupled receptors, which mediate cell-cell
            interactions. These proteins are cleaved by self-catalytic
            proteolysis into a large extracellular subunit and seven-span
            transmembrane subunit, which associate at the cell surface as a
            receptor complex. The encoded protein may play a role in cell
            adhesion as well as leukocyte recruitment, activation and
            migration, and contains multiple extracellular EGF-like repeats
            which mediate binding to chondroitin sulfate and the cell surface
            complement regulatory protein CD55. Expression of this gene may
            play a role in the progression of several types of cancer.
            Alternatively spliced transcript variants encoding multiple
            isoforms with 3 to 5 EGF-like repeats have been observed for this
            gene. This gene is found in a cluster with other EGF-TM7 genes on
            the short arm of chromosome 19. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1, also known as
            CD97(EGF 1,2,3,4,5)).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK314697.1, SRR1660805.83561.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000242786.6/ ENSP00000242786.4
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2588              AK314697.1         1-2588
            2589-3188           U76764.1           2557-3156
            3189-3190           AI890331.1         11-12               c
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..3190
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /note="adhesion G protein-coupled receptor E5"
                     /db_xref="GeneID:976"
                     /db_xref="HGNC:HGNC:1711"
                     /db_xref="MIM:601211"
     exon            1..102
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..2588
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; leukocyte antigen CD97; seven-span
                     transmembrane protein; seven-transmembrane, heterodimeric
                     receptor associated with inflammation; seven transmembrane
                     helix receptor; CD97 molecule"
                     /codon_start=1
                     /product="adhesion G protein-coupled receptor E5 isoform 1
                     preproprotein"
                     /protein_id="NP_510966.1"
                     /db_xref="CCDS:CCDS32929.1"
                     /db_xref="GeneID:976"
                     /db_xref="HGNC:HGNC:1711"
                     /db_xref="MIM:601211"
                     /translation="MGGRVFLAFCVWLTLPGAETQDSRGCARWCPQNSSCVNATACRC
                     NPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSG
                     AKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCLPGFKFIPEDPKVCTD
                     VNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDECSSGQH
                     QCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLS
                     RFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNL
                     EDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAE
                     DPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLSAVNSIFL
                     SHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQELLCAFWKSDSDRGGHW
                     ATEGCQVLGSKNGSTTCQCSHLSSFAILMAHYDVEDWKLTLITRVGLALSLFCLLLCI
                     LTFLLVRPIQGSRTTIHLHLCICLFVGSTIFLAGIENEGGQVGLRCRLVAGLLHYCFL
                     AAFCWMSLEGLELYFLVVRVFQGQGLSTRWLCLIGYGVPLLIVGVSAAIYSKGYGRPR
                     YCWLDFEQGFLWSFLGPVTFIILCNAVIFVTTVWKLTQKFSEINPDMKKLKKARALTI
                     TAIAQLFLLGCTWVFGLFIFDDRSLVLTYVFTILNCLQGAFLYLLHCLLNKKVREEYR
                     KWACLVAGGSKYSEFTSTTSGTGHNQTRALRASESGI"
     exon            103..153
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            154..270
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            271..426
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            427..558
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            559..705
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..852
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            853..899
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..977
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1155
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1263
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1529
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1709
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1901
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2128
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2195
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2287
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2456
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2558
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..3190
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3170..3175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /note="hexamer: ATTAAA"
     polyA_site      3190
                     /gene="ADGRE5"
                     /gene_synonym="CD97; TM7LN1"
                     /note="major polyA site"
ORIGIN      
        1 acagctgcct agcctgtgga gacgggacag ccctgtccca ctcactcttt cccctgccgc
       61 tcctgccggc agctccaacc atgggaggcc gcgtctttct cgcattctgt gtctggctga
      121 ctctgccggg agctgaaacc caggactcca ggggctgtgc ccggtggtgc cctcagaact
      181 cctcgtgtgt caatgccacc gcctgtcgct gcaatccagg gttcagctct ttttctgaga
      241 tcatcaccac cccgacggag acttgtgacg acatcaacga gtgtgcaaca ccgtcgaaag
      301 tgtcatgcgg aaaattctcg gactgctgga acacagaggg gagctacgac tgcgtgtgca
      361 gcccgggata tgagcctgtt tctggggcaa aaacattcaa gaatgagagc gagaacacct
      421 gtcaagatgt ggacgaatgt cagcagaacc caaggctctg taaaagctac ggcacctgcg
      481 tcaacaccct tggcagctat acctgccagt gcctgcctgg cttcaagttc atacctgagg
      541 atccgaaggt ctgcacagat gtgaatgaat gcacctccgg acaaaacccg tgccacagct
      601 ccacccactg cctcaacaac gtgggcagct atcagtgccg ctgccgcccg ggctggcaac
      661 cgattccggg gtcccccaat ggcccaaaca ataccgtctg tgaagatgtg gacgagtgca
      721 gctccgggca gcatcagtgt gacagctcca ccgtctgctt caacaccgtg ggttcataca
      781 gctgccgctg ccgcccaggc tggaagccca gacacggaat cccgaataac caaaaggaca
      841 ctgtctgtga agatatgact ttctccacct ggaccccgcc ccctggagtc cacagccaga
      901 cgctttcccg attcttcgac aaagtccagg acctgggcag agactccaag acaagctcag
      961 ccgaggtcac catccagaat gtcatcaaat tggtggatga actgatggaa gctcctggag
     1021 acgtagaggc cctggcgcca cctgtccggc acctcatagc cacccagctg ctctcaaacc
     1081 ttgaagatat catgaggatc ctggccaaga gcctgcctaa aggccccttc acctacattt
     1141 ccccttcgaa cacagagctg accctgatga tccaggagcg gggggacaag aacgtcacta
     1201 tgggtcagag cagcgcacgc atgaagctga attgggctgt ggcagctgga gccgaggatc
     1261 caggccccgc cgtggcgggc atcctctcca tccagaacat gacgacattg ctggccaatg
     1321 cctccttgaa cctgcattcc aagaagcaag ccgaactgga ggagatatat gaaagcagca
     1381 tccgtggtgt ccaactcaga cgcctctctg ccgtcaactc catctttctg agccacaaca
     1441 acaccaagga actcaactcc cccatccttt tcgccttctc ccaccttgag tcctccgatg
     1501 gggaggcggg aagagaccct cctgccaagg acgtgatgcc tgggccacgg caggagctgc
     1561 tctgtgcctt ctggaagagt gacagcgaca ggggagggca ctgggccacc gagggctgcc
     1621 aggtgctggg cagcaagaac ggcagcacca cctgccaatg cagccacctg agcagctttg
     1681 cgatccttat ggctcattat gacgtggagg actggaagct gaccctgatc accagggtgg
     1741 gactggcgct gtcactcttc tgcctgctgc tgtgcatcct cactttcctg ctggtgcggc
     1801 ccatccaggg ctcgcgcacc accatacacc tgcacctctg catctgcctc ttcgtgggct
     1861 ccaccatctt cctggccggc atcgagaacg aaggcggcca ggtggggctg cgctgccgcc
     1921 tggtggccgg gctgctgcac tactgtttcc tggccgcctt ctgctggatg agcctcgaag
     1981 gcctggagct ctactttctt gtggtgcgcg tgttccaagg ccagggcctg agtacgcgct
     2041 ggctctgcct gatcggctat ggcgtgcccc tgctcatcgt gggcgtctcg gctgccatct
     2101 acagcaaggg ctacggccgc cccagatact gctggttgga ctttgagcag ggcttcctct
     2161 ggagcttctt gggacctgtg accttcatca ttttgtgcaa tgctgtcatt ttcgtgacta
     2221 ccgtctggaa gctcactcag aagttttctg aaatcaatcc agacatgaag aaattaaaga
     2281 aggcgagggc gctgaccatc acggccatcg cgcagctctt cctgttgggc tgcacctggg
     2341 tctttggcct gttcatcttc gacgatcgga gcttggtgct gacctatgtg tttaccatcc
     2401 tcaactgcct gcagggcgcc ttcctctacc tgctgcactg cctgctcaac aagaaggttc
     2461 gggaagaata ccggaagtgg gcctgcctag ttgctggggg gagcaagtac tcagaattca
     2521 cctccaccac gtctggcact ggccacaatc agacccgggc cctcagggca tcagagtccg
     2581 gcatatgaag gcgcatggtt ctggacggcc cagcagctcc tgtggccaca gcagctttgt
     2641 acacgaagac catccatcct cccttcgtcc accactctac tccctccacc ctccctccct
     2701 gatcccgtgt gccaccagga gggagtggca gctatagtct ggcaccaaag tccaggacac
     2761 ccagtggggt ggagtcggag ccactggtcc tgctgctggc tgcctctctg ctccaccttg
     2821 tgacccaggg tggggacagg ggctggccca gggctgcaat gcagcatgtt gccctggcac
     2881 ctgtggccag tactcgggac agactaaggg cgcttgtccc atcctggact tttcctctca
     2941 tgtctttgct gcagaactga agagactagg cgctggggct cagcttccct cttaagctaa
     3001 gactgatgtc agaggcccca tggcgaggcc ccttggggcc actgcctgag gctcacggta
     3061 cagaggcctg ccctgcctgg ccgggcagga ggttctcact gttgtgaagg ttgtagacgt
     3121 tgtgtaatgt gtttttatct gttaaaattt ttcagtgttg acacttaaaa ttaaacacat
     3181 gcatacagaa
//
