LOCUS       NM_013447               6791 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens adhesion G protein-coupled receptor E2 (ADGRE2),
            transcript variant 1, mRNA.
ACCESSION   NM_013447
VERSION     NM_013447.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Huang,D., Yu,Z., Lu,H., Jiang,P., Qian,X., Han,Y. and Qian,P.
  TITLE     Adhesion GPCR ADGRE2 Maintains Proteostasis to Promote Progression
            in Acute Myeloid Leukemia
  JOURNAL   Cancer Res 84 (13), 2090-2108 (2024)
   PUBMED   39082681
  REMARK    GeneRIF: Adhesion GPCR ADGRE2 Maintains Proteostasis to Promote
            Progression in Acute Myeloid Leukemia.
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Zheng,L., Rang,M., Fuchs,C., Kess,A., Wunsch,M., Hentschel,J.,
            Hsiao,C.C., Kleber,C., Osterhoff,G. and Aust,G.
  TITLE     The Posttraumatic Increase of the Adhesion GPCR EMR2/ADGRE2 on
            Circulating Neutrophils Is Not Related to Injury Severity
  JOURNAL   Cells 12 (22), 2657 (2023)
   PUBMED   37998392
  REMARK    GeneRIF: The Posttraumatic Increase in the Adhesion of GPCR
            EMR2/ADGRE2 to Circulating Neutrophils Is Not Related to Injury
            Severity.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6791)
  AUTHORS   Beliu,G., Altrichter,S., Guixa-Gonzalez,R., Hemberger,M.,
            Brauer,I., Dahse,A.K., Scholz,N., Wieduwild,R., Kuhlemann,A.,
            Batebi,H., Seufert,F., Perez-Hernandez,G., Hildebrand,P.W.,
            Sauer,M. and Langenhan,T.
  TITLE     Tethered agonist exposure in intact adhesion/class B2 GPCRs through
            intrinsic structural flexibility of the GAIN domain
  JOURNAL   Mol Cell 81 (5), 905-921 (2021)
   PUBMED   33497605
  REMARK    GeneRIF: Tethered agonist exposure in intact adhesion/class B2
            GPCRs through intrinsic structural flexibility of the GAIN domain.
REFERENCE   4  (bases 1 to 6791)
  AUTHORS   Chiu,P.L., Ng,B.H., Chang,G.W., Gordon,S. and Lin,H.H.
  TITLE     Putative alternative trans-splicing of leukocyte adhesion-GPCR
            pre-mRNAs generates functional chimeric receptors
  JOURNAL   FEBS Lett 582 (5), 792-798 (2008)
   PUBMED   18267122
  REMARK    GeneRIF: EGF-TM7 pre-mRNAs also undergo the rare trans-splicing,
            leading to the generation of functional chimeric receptors.
REFERENCE   5  (bases 1 to 6791)
  AUTHORS   Bjarnadottir,T.K., Geirardsdottir,K., Ingemansson,M., Mirza,M.A.,
            Fredriksson,R. and Schioth,H.B.
  TITLE     Identification of novel splice variants of Adhesion G
            protein-coupled receptors
  JOURNAL   Gene 387 (1-2), 38-48 (2007)
   PUBMED   17056209
REFERENCE   6  (bases 1 to 6791)
  AUTHORS   Aust,G., Hamann,J., Schilling,N. and Wobus,M.
  TITLE     Detection of alternatively spliced EMR2 mRNAs in colorectal tumor
            cell lines but rare expression of the molecule in colorectal
            adenocarcinomas
  JOURNAL   Virchows Arch 443 (1), 32-37 (2003)
   PUBMED   12761622
REFERENCE   7  (bases 1 to 6791)
  AUTHORS   Aust,G., Steinert,M., Schutz,A., Boltze,C., Wahlbuhl,M., Hamann,J.
            and Wobus,M.
  TITLE     CD97, but not its closely related EGF-TM7 family member EMR2, is
            expressed on gastric, pancreatic, and esophageal carcinomas
  JOURNAL   Am J Clin Pathol 118 (5), 699-707 (2002)
   PUBMED   12428789
REFERENCE   8  (bases 1 to 6791)
  AUTHORS   Kwakkenbos,M.J., Chang,G.W., Lin,H.H., Pouwels,W., de Jong,E.C.,
            van Lier,R.A., Gordon,S. and Hamann,J.
  TITLE     The human EGF-TM7 family member EMR2 is a heterodimeric receptor
            expressed on myeloid cells
  JOURNAL   J Leukoc Biol 71 (5), 854-862 (2002)
   PUBMED   11994511
REFERENCE   9  (bases 1 to 6791)
  AUTHORS   Lin,H.H., Stacey,M., Saxby,C., Knott,V., Chaudhry,Y., Evans,D.,
            Gordon,S., McKnight,A.J., Handford,P. and Lea,S.
  TITLE     Molecular analysis of the epidermal growth factor-like short
            consensus repeat domain-mediated protein-protein interactions:
            dissection of the CD97-CD55 complex
  JOURNAL   J Biol Chem 276 (26), 24160-24169 (2001)
   PUBMED   11297558
REFERENCE   10 (bases 1 to 6791)
  AUTHORS   Lin,H.H., Stacey,M., Hamann,J., Gordon,S. and McKnight,A.J.
  TITLE     Human EMR2, a novel EGF-TM7 molecule on chromosome 19p13.1, is
            closely related to CD97
  JOURNAL   Genomics 67 (2), 188-200 (2000)
   PUBMED   10903844
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005327.1, BG721006.1,
            BC127004.1, AK298700.1, AC090427.2 and CN480316.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_013447.3.
            
            Summary: This gene encodes a member of the class B seven-span
            transmembrane (TM7) subfamily of G-protein coupled receptors. These
            proteins are characterized by an extended extracellular region with
            a variable number of N-terminal epidermal growth factor-like
            domains coupled to a TM7 domain via a mucin-like spacer domain. The
            encoded protein is expressed mainly in myeloid cells where it
            promotes cell-cell adhesion through interaction with chondroitin
            sulfate chains. This gene is situated in a cluster of related genes
            on chromosome 19. Alternatively spliced transcript variants
            encoding multiple isoforms have been observed for this gene.
            [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC127004.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000315576.8/ ENSP00000319883.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC005327.1         26381-26399         c
            20-23               BG721006.1         6-9
            24-894              BC127004.1         1-871
            895-1416            AK298700.1         507-1028
            1417-3104           BC127004.1         1394-3081
            3105-5994           AC090427.2         83157-86046         c
            5995-6486           CN480316.1         17-508              c
            6487-6791           AC090427.2         82360-82664         c
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..6791
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="adhesion G protein-coupled receptor E2"
                     /db_xref="GeneID:30817"
                     /db_xref="HGNC:HGNC:3337"
                     /db_xref="MIM:606100"
     exon            1..304
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            305..506
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     CDS             476..2947
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; EGF-like module-containing mucin-like hormone
                     receptor-like 2; egf-like module containing, mucin-like,
                     hormone receptor-like 2; CD97 antigen; Leukocyte antigen
                     CD97"
                     /codon_start=1
                     /product="adhesion G protein-coupled receptor E2 isoform a
                     precursor"
                     /protein_id="NP_038475.2"
                     /db_xref="CCDS:CCDS32935.1"
                     /db_xref="GeneID:30817"
                     /db_xref="HGNC:HGNC:3337"
                     /db_xref="MIM:606100"
                     /translation="MGGRVFLVFLAFCVWLTLPGAETQDSRGCARWCPQDSSCVNATA
                     CRCNPGFSSFSEIITTPMETCDDINECATLSKVSCGKFSDCWNTEGSYDCVCSPGYEP
                     VSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCLPGFKLKPEDPKL
                     CTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDECSS
                     GQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQ
                     TLSRFFDKVQDLGRDYKPGLANNTIQSILQALDELLEAPGDLETLPRLQQHCVASHLL
                     DGLEDVLRGLSKNLSNGLLNFSYPAGTELSLEVQKQVDRSVTLRQNQAVMQLDWNQAQ
                     KSGDPGPSVVGLVSIPGMGKLLAEAPLVLEPEKQMLLHETHQGLLQDGSPILLSDVIS
                     AFLSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQNGCGHWATTGCSTIGTRDT
                     STICRCTHLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALTFLLCKAIQN
                     TSTSLHLQLSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLLEALYL
                     FLTARNLTVVNYSSINRFMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSRCWLQPEK
                     GFIWGFLGPVCAIFSVNLVLFLVTLWILKNRLSSLNSEVSTLRNTRMLAFKATAQLFI
                     LGCTWCLGILQVGPAARVMAYLFTIINSLQGVFIFLVYCLLSQQVREQYGKWSKGIRK
                     LKTESEMHTLSSSAKADTSKPSTVN"
     exon            507..557
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            558..674
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            675..830
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            831..962
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1109
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1256
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1303
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1381
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1559
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1667
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1891
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2065
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2263
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2264..2499
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2566
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2567..2658
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2827
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2828..2938
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     exon            2939..6791
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6367..6372
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="hexamer: AATAAA"
     polyA_site      6389
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
     regulatory      6458..6463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="hexamer: AATAAA"
     polyA_site      6479
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="major polyA site"
     regulatory      6769..6774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
                     /note="hexamer: AATAAA"
     polyA_site      6791
                     /gene="ADGRE2"
                     /gene_synonym="CD312; CD97; EMR2; VBU"
ORIGIN      
        1 agaaagacca gaaagatccg gggggtctgc tctgtgcggt gaagcttctc ttcttggcac
       61 ctgcctggca tcggaagagg gccccttctc cctccctggg cttttatgtg gacactgtaa
      121 tgcctcagtt ttctttcttt ctttttgttt ttgacacagg gtctcactct atcacccaag
      181 ctggagtgca gtggcaccat cttagctcac cgcagcctca aactccccag ctcgggtgat
      241 tcttcctgcc tcagcctcct gagtagtagc agctgggacc acagacgtct gccaccaagc
      301 ccagctgcac cgccagttcc ggggagggcc ctgggccagc ggctgtccgc cccccctcct
      361 ttataaagtc ctggcctcgg gacagcccgc acagctgccc agcctgcgga gacgggacag
      421 ccctgtccca ctcactcttt cccctgctgc tcctgccggc agctcagctg gaaccatggg
      481 aggccgcgtc tttctcgtct ttctcgcatt ctgtgtctgg ctgactctgc cgggagctga
      541 aacccaggac tccaggggct gtgcccggtg gtgccctcag gactcctcgt gtgtcaatgc
      601 caccgcctgt cgctgcaatc cagggttcag ctctttttct gagatcatca ccacccccat
      661 ggagacttgt gacgacatca acgagtgtgc aacactgtcg aaagtgtcat gcggaaaatt
      721 ctcggactgc tggaacacag aggggagcta cgactgcgtg tgcagcccag gatatgagcc
      781 tgtttctggg gcaaaaacat tcaagaatga gagcgagaac acgtgtcaag atgtggacga
      841 atgtcagcag aacccaaggc tctgtaaaag ctacggcacc tgcgtcaaca ccctcggcag
      901 ctacacgtgc cagtgcctgc ctggcttcaa gctcaaacct gaggacccga agctctgcac
      961 agatgtgaat gaatgcacct ccggacaaaa cccatgccac agctccaccc actgcctcaa
     1021 caacgtgggc agctatcagt gccgctgccg cccgggctgg caaccgattc cggggtcccc
     1081 caatggccca aacaataccg tctgtgaaga tgtggacgag tgcagctccg ggcagcatca
     1141 gtgtgacagc tccaccgtct gcttcaacac cgtgggttca tacagctgcc gctgccgccc
     1201 aggctggaag cccagacacg gaatcccgaa taaccaaaag gacactgtct gtgaagatat
     1261 gactttctcc acctggaccc cgccccctgg agtccacagc cagacgcttt cccgattctt
     1321 cgacaaagtc caggacctgg gcagagacta caagccaggc ttggccaata acaccatcca
     1381 gagcatctta caggcgctgg atgagctgct ggaggcccct ggggacctgg agaccctgcc
     1441 ccgcttacag cagcactgtg tggccagtca cctgctggat ggcctagagg atgtcctcag
     1501 aggcctgagc aagaaccttt ccaatgggct gttgaacttc agttatcctg caggcacaga
     1561 attgtccctg gaggtgcaga agcaagtaga caggagtgtc accttgagac agaatcaggc
     1621 agtgatgcag ctcgactgga atcaggcaca gaaatctggt gacccaggcc cttctgtggt
     1681 gggccttgtc tccattccag ggatgggcaa gttgctggct gaggcccctc tggtcctgga
     1741 acctgagaag cagatgcttc tgcatgagac acaccagggc ttgctgcagg acggctcccc
     1801 catcctgctc tcagatgtga tctctgcctt tctgagcaac aacgacaccc aaaacctcag
     1861 ctccccagtt accttcacct tctcccaccg ttcagtgatc ccgagacaga aggtgctctg
     1921 tgtcttctgg gagcatggcc agaatggatg tggtcactgg gccaccacag gctgcagcac
     1981 aataggcacc agagacacca gcaccatctg ccgttgcacc cacctgagca gctttgccgt
     2041 cctcatggcc cactacgatg tgcaggagga ggatcccgtg ctgactgtca tcacctacat
     2101 ggggctgagc gtctctctgc tgtgcctcct cctggcggcc ctcacttttc tcctgtgtaa
     2161 agccatccag aacaccagca cctcactgca tctgcagctc tcgctctgcc tcttcctggc
     2221 ccacctcctc ttcctcgtgg caattgatca aaccggacac aaggtgctgt gctccatcat
     2281 cgccggtacc ttgcactatc tctacctggc caccttgacc tggatgctgc tggaggccct
     2341 gtacctcttc ctcactgcac ggaacctgac ggtggtcaac tactcaagca tcaacagatt
     2401 catgaagaag ctcatgttcc ctgtgggcta cggagtccca gctgtgacag tggccatttc
     2461 tgcagcctcc aggcctcacc tttatggaac accttcccgc tgctggctcc aaccagaaaa
     2521 gggatttata tggggcttcc ttggacctgt ctgcgccatc ttctctgtga atttagttct
     2581 ctttctggtg actctctgga ttttgaaaaa cagactctcc tccctcaata gtgaagtgtc
     2641 caccctccgg aacacaagga tgctggcatt taaagcgaca gctcagctgt tcatcctggg
     2701 ctgcacgtgg tgtctgggca tcttgcaggt gggtccggct gcccgggtca tggcctacct
     2761 cttcaccatc atcaacagcc tgcagggtgt cttcatcttc ctggtgtact gcctcctcag
     2821 ccagcaggtc cgggagcaat atgggaaatg gtccaaaggg atcaggaaat tgaaaactga
     2881 gtctgagatg cacacactct ccagcagtgc taaggctgac acctccaaac ccagcacggt
     2941 taactagaaa aatcttctga ataagatctt ccctctttgc ccgtggaaaa tctgaacaat
     3001 ctttgagcca tctagagggg aaagaaaaga ctttgttctg tgtgtttcaa gaaattcacc
     3061 atgtcagcaa tatgaaggat gttatggaag gcgtgctagg cattcaattc ctgcagaaac
     3121 cggaaatctt ccatgccctg caatgtgctc atcaaactct cagcatatgg acggccagct
     3181 gtggcccata tcttggtcac tctgaagcac aatatttatg aagctataga acgttaagac
     3241 ctctttcaca gcctctcctt cctacaaaga ctcctccaaa tcttaaaatg aagcaggaaa
     3301 acgagcctaa gaggactttc ataccgacaa catctgaaag gactagaatg ttcacaccac
     3361 gatctggatt tcttaatttt ttgtttttgt ttttgttgtt ctctagttct acgggtttga
     3421 ttatttagtc atgtgaaaaa tattgattac tcacacatag atcaagagag acacggctcc
     3481 tgccttcatg gagcttttag gggaaaatga agtggctctt gcagctagag ttgactcaga
     3541 agccgaaatt cctagaaatc aggtttctac tgctaggcaa ttgaagtata aactatttta
     3601 taaacactgt cttctttcgt cttcacacca acatgcagaa aagtttctaa tctcagatcg
     3661 gggatgtgca acaaattcca tttcaaagga atgacctgca aaactcctaa atattccaag
     3721 caaatgccct taaccctgtc tgttatctgc tttccttgaa cagaaattct acatgaccat
     3781 aaaacctcga agatgggtat ggcacagttc atgccctgta atcctagcac tttgggaggg
     3841 tgaggcagga ggatggctca agcccaggag tttgagacca gtgtgggcaa cagagtgaga
     3901 accatctcta cccaaaaaaa aaattaaaaa ttagccaagc atggtgatga tataggagtt
     3961 aaggagaaat catttaggca aatagcaagg gtaggaagtc ctcagtaagg ttttccattt
     4021 aatgaaaagc agcccccaaa atcattttct tttctaacaa agaacagcct gtaaaatcga
     4081 gctgcagaca tagacaagca agctggaagc ttccacgggt gaatgccggc agctgtgcca
     4141 ataggaaaaa gctacctaga ctaggcatgt ccaaaatggc ggctccaagt tcccttctct
     4201 ttgccagcca tgtgtacagt aaaaagcagg caacatagtg tcagccaaag ctcatttgca
     4261 taataagatt agggtggggt ggccagctca cataggggta ggccctaggt aaatcagaca
     4321 ccgccttctc aagcctgtct ataaaatctg gtacactatg acgagggtca gatttcccat
     4381 tcagacgccc ctctcccatg caagagaaag agctgttctc ctttctcttt cttttgccta
     4441 ttaaacctct gctcctggcc aggcacagtg gctcacgcct ataatcccag cactttggga
     4501 ggctgaggtg gtcagatcac ctaaggtcag gagttcaaga ccagcctggt caacatggtg
     4561 aaatcttgtc tctagtaaaa atacaaaaat atatgaaatc tcacatagat gataatatta
     4621 agttccaaaa gcaactcaac ctggtagatt ctaatttttt ttgaggcagg gtcttgcttt
     4681 gtcacccatg ctggagtaca atggcacaaa cactgctcac tgcagcctcg acctcccaag
     4741 gcctaagcaa tcctcctgcc tcagtccccc tccaggtatt tgaaactaca ggtgtgtacc
     4801 accacaccgg gctaattttt gtattttttg tagagacgtg ggtctcacta tgctgcccag
     4861 gctcaggtct taatctcctg agctcaggca atccgcaggc ctcagcctcc ctaagtgcgg
     4921 ggattacagg cttgagccac tgcacctagc ctctatttgt tttacaaaag agaaattgag
     4981 atcctgaatg ttaagtgact tgcctgaggc catcccacta acaggagcca gggttaggat
     5041 tcaaacccca tccaactggt cccagagctg gagcttcttg cactgcccta cactacctac
     5101 catctccatc ctctgggcac ctttttataa gaaccaaaac attacagagc attgctttgt
     5161 caactcagct gggaacattt cccagtgcaa ctcacatttt tcactgctct gtgcctgtcc
     5221 gtataagctc aatgagtatt gatttagggg ctttggagaa ctttgaatgc taccccccaa
     5281 gtaaccattg ttggcaacct ggtacctcta cttttagcca tttctccttc tctataaata
     5341 gtgcagaagt aacccacttg gtaacaggca tccttgccaa gcctccacca ctaggtcagt
     5401 gtaagaatta aagaaagagg aaagaaacac aaaaagtggc ttgatggtta agacaggttt
     5461 attttagaga aaacacacct gagaggggct gctggctgaa ttaggttaga gtcttttcta
     5521 cagactaaga gtgtttaagg atttagggtg ggagagtttc ttagaggctt ggactgcttc
     5581 tgtgtttttt ttgttgtgct tatatgggag ggagagtggt gtgtttgctt ttatacattt
     5641 ttctgcagct gtaggcatac cccccaagtc tgcttttagc ttccctattt tagtgcacct
     5701 ggagggaaag gaatgtgctt attaaggccc actgttttac tggggcccat tgtatgaggg
     5761 tgaagtttgg cagttaccca agagactttt cctccacctt cctctgtgcc cgagctgttt
     5821 tatctgcatt ttactgtctg ctttttttgg ctgcttatag tttttaaaaa agtaatttcc
     5881 ttaaatccag aaggctaaaa atgaagctga aacttaaagt ggcggtgttt gtccaaaata
     5941 acggggctcc tgctctgcca gtcagtaccc tcaagtcact cctgatcctc aacctccatg
     6001 cctaaggctg gttcaagaga ccacataata tctgcctttt attacataca tgatgggtgc
     6061 atgggattct gcgtgccctt tgcttgatat agactgctaa ggtgagatgg ggaatatcag
     6121 agtcagctgc tgcttgagga agcagaacac acagctggag gcttggaaca tgtgggtccc
     6181 tatgagtgta gagcccatat ccccatagag tctacctaga gcaggggtcg ccaaatgttt
     6241 tcttaaagag cctgatagtg tatatgttag gctttgtgag ccaggtattt acagcaactc
     6301 aattctacca ctgtggtatg aaaacagcta tagacaatca taaatgaatg atcatggcta
     6361 tgttttaata aaactttaca gacactgaac ttgaacttcc attgtgatat gaaaacagct
     6421 atagacaatc ataaatgaat gatcatggct atgttttaat aaaactttat ggacactgag
     6481 cttgaacttc atatatcagt catgtgacac aaaatatcat tcttcttttt atttgttttc
     6541 acccatttga aaaatgtaaa aactattctt agctgtacag aaacagatgg tgggtcagat
     6601 ttgactcact gcccatagtt tccagaccat gatgttcagg ttcatggaag cacttcactc
     6661 tacactaatt tattcatcca ttcatcacat atgtgatgag cacatacaga cacacctcag
     6721 agatattgca ggctccactc cagatcacca caataatgtg aatattgcaa taaagcaagt
     6781 catacacatt t
//
