LOCUS       NM_001115               4421 bp    mRNA    linear   PRI 27-APR-2025
DEFINITION  Homo sapiens adenylate cyclase 8 (ADCY8), mRNA.
ACCESSION   NM_001115
VERSION     NM_001115.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Tabbasum,V.G. and Cooper,D.M.F.
  TITLE     Structural and Functional Determinants of AC8 Trafficking,
            Targeting and Responsiveness in Lipid Raft Microdomains
  JOURNAL   J Membr Biol 252 (2-3), 159-172 (2019)
   PUBMED   30746562
  REMARK    GeneRIF: Colocalization of caveolin1 and adenylyl cyclase 8 in
            lipid rafts depends on the cytoskeleton.
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Shen-Gunther,J., Wang,C.M., Poage,G.M., Lin,C.L., Perez,L.,
            Banks,N.A. and Huang,T.H.
  TITLE     Molecular Pap smear: HPV genotype and DNA methylation of ADCY8,
            CDH8, and ZNF582 as an integrated biomarker for high-grade cervical
            cytology
  JOURNAL   Clin Epigenetics 8 (1), 96 (2016)
   PUBMED   27651839
  REMARK    GeneRIF: Results show that promoter hypermethylation of ADCY8,
            CDH8, and ZNF582 are correlated with high-grade squamous
            intraepithelial lesion.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4421)
  AUTHORS   Sung,Y.J., Perusse,L., Sarzynski,M.A., Fornage,M., Sidney,S.,
            Sternfeld,B., Rice,T., Terry,J.G., Jacobs,D.R. Jr., Katzmarzyk,P.,
            Curran,J.E., Jeffrey Carr,J., Blangero,J., Ghosh,S., Despres,J.P.,
            Rankinen,T., Rao,D.C. and Bouchard,C.
  TITLE     Genome-wide association studies suggest sex-specific loci
            associated with abdominal and visceral fat
  JOURNAL   Int J Obes (Lond) 40 (4), 662-674 (2016)
   PUBMED   26480920
  REMARK    GeneRIF: Our results provide evidence for new loci influencing
            abdominal visceral (BBS9, ADCY8, KCNK9) and subcutaneous
            (MLLT10/DNAJC1/EBLN1) fat, and confirmed a locus (THNSL2)
            previously reported to be associated with abdominal fat in women
REFERENCE   4  (bases 1 to 4421)
  AUTHORS   Raoux,M., Vacher,P., Papin,J., Picard,A., Kostrzewa,E., Devin,A.,
            Gaitan,J., Limon,I., Kas,M.J., Magnan,C. and Lang,J.
  TITLE     Multilevel control of glucose homeostasis by adenylyl cyclase 8
  JOURNAL   Diabetologia 58 (4), 749-757 (2015)
   PUBMED   25403481
  REMARK    GeneRIF: ADCY8 is required for the physiological activation of
            glucose-induced signalling pathways in beta cells, for glucose
            tolerance and for hypothalamic adaptation to a high-fat diet via
            regulation of islet insulin secretion.
REFERENCE   5  (bases 1 to 4421)
  AUTHORS   Cobb,J., Cule,E., Moncrieffe,H., Hinks,A., Ursu,S., Patrick,F.,
            Kassoumeri,L., Flynn,E., Bulatovic,M., Wulffraat,N., van Zelst,B.,
            de Jonge,R., Bohm,M., Dolezalova,P., Hirani,S., Newman,S.,
            Whitworth,P., Southwood,T.R., De Iorio,M., Wedderburn,L.R. and
            Thomson,W.
  CONSRTM   Childhood Arthritis Response to Medication Study (CHARMS);
            Childhood Arthritis Prospective Study (CAPS); BSPAR study group
  TITLE     Genome-wide data reveal novel genes for methotrexate response in a
            large cohort of juvenile idiopathic arthritis cases
  JOURNAL   Pharmacogenomics J 14 (4), 356-364 (2014)
   PUBMED   24709693
REFERENCE   6  (bases 1 to 4421)
  AUTHORS   Kleuss,C., Raw,A.S., Lee,E., Sprang,S.R. and Gilman,A.G.
  TITLE     Mechanism of GTP hydrolysis by G-protein alpha subunits
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 9828-9831 (1994)
   PUBMED   7937899
REFERENCE   7  (bases 1 to 4421)
  AUTHORS   Defer,N., Marinx,O., Stengel,D., Danisova,A., Iourgenko,V.,
            Matsuoka,I., Caput,D. and Hanoune,J.
  TITLE     Molecular cloning of the human type VIII adenylyl cyclase
  JOURNAL   FEBS Lett 351 (1), 109-113 (1994)
   PUBMED   8076676
REFERENCE   8  (bases 1 to 4421)
  AUTHORS   Stengel,D., Parma,J., Gannage,M.H., Roeckel,N., Mattei,M.G.,
            Barouki,R. and Hanoune,J.
  TITLE     Different chromosomal localization of two adenylyl cyclase genes
            expressed in human brain
  JOURNAL   Hum Genet 90 (1-2), 126-130 (1992)
   PUBMED   1427768
REFERENCE   9  (bases 1 to 4421)
  AUTHORS   Parma,J., Stengel,D., Gannage,M.H., Poyard,M., Barouki,R. and
            Hanoune,J.
  TITLE     Sequence of a human brain adenylyl cyclase partial cDNA: evidence
            for a consensus cyclase specific domain
  JOURNAL   Biochem Biophys Res Commun 179 (1), 455-462 (1991)
   PUBMED   1715695
REFERENCE   10 (bases 1 to 4421)
  AUTHORS   Taylor,S.S., Buechler,J.A. and Yonemoto,W.
  TITLE     cAMP-dependent protein kinase: framework for a diverse family of
            regulatory enzymes
  JOURNAL   Annu Rev Biochem 59, 971-1005 (1990)
   PUBMED   2165385
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087341.4, DR003657.1,
            Z35309.1, DQ104739.1 and AC103726.5.
            
            On Nov 22, 2018 this sequence version replaced NM_001115.2.
            
            Summary: Adenylate cyclase is a membrane bound enzyme that
            catalyses the formation of cyclic AMP from ATP. The enzymatic
            activity is under the control of several hormones, and different
            polypeptides participate in the transduction of the signal from the
            receptor to the catalytic moiety. Stimulatory or inhibitory
            receptors (Rs and Ri) interact with G proteins (Gs and Gi) that
            exhibit GTPase activity and they modulate the activity of the
            catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GQ472223.1, Z35309.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154665 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000286355.10/ ENSP00000286355.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-320               AC087341.4         62782-63101         c
            321-741             DR003657.1         9-429
            742-2142            Z35309.1           2260-3660
            2143-2213           DQ104739.1         1567-1637
            2214-2216           AC103726.5         89500-89502
            2217-4360           Z35309.1           3735-5878
            4361-4421           Z35309.1           5882-5942
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.22"
     gene            1..4421
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /note="adenylate cyclase 8"
                     /db_xref="GeneID:114"
                     /db_xref="HGNC:HGNC:239"
                     /db_xref="MIM:103070"
     exon            1..1536
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             577..4332
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /EC_number="4.6.1.1"
                     /note="HEL-S-172mP; epididymis secretory sperm binding
                     protein Li 172mP; adenylyl cyclase-8, brain; adenylyl
                     cyclase 8; ATP pyrophosphate-lyase 8; adenylate cyclase
                     type VIII; ca(2+)/calmodulin-activated adenylyl cyclase;
                     adenylate cyclase 8 (brain)"
                     /codon_start=1
                     /product="adenylate cyclase type 8"
                     /protein_id="NP_001106.1"
                     /db_xref="CCDS:CCDS6363.1"
                     /db_xref="GeneID:114"
                     /db_xref="HGNC:HGNC:239"
                     /db_xref="MIM:103070"
                     /translation="MELSDVRCLTGSEELYTIHPTPPAGDGRSASRPQRLLWQTAVRH
                     ITEQRFIHGHRGGSGSGSGGSGKASDPAGGGPNHHAPQLSGDSALPLYSLGPGERAHS
                     TCGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNS
                     FKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGF
                     FTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLF
                     TLFATYSMLPLPLTWAILAGLGTSLLQVILQVVIPRLAVISINQVVAQAVLFMCMNTA
                     GIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTN
                     VEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLA
                     HEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRYVRSRTKHDVDMRI
                     GIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGDYNVE
                     EGHGKERNEFLRKHNIETYLIKQPEDSLLSLPEDIVKESVSSSDRRNSGATFTEGSWS
                     PELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDK
                     LRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRVMP
                     MTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETYLARNVIIFASILINFL
                     GAILNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSV
                     LKLAVLLIMIAIYALLTETVYAGLFLRYDNLNHSGEDFLGTKEVSLLLMAMFLLAVFY
                     HGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEE
                     LYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDI
                     EKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELR
                     IGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFA
                     FDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLN
                     RQRQKQLLNENNNTGIIKGHYNRRTLLSPSGTEPGAQAEGTDKSDLP"
     exon            1537..1686
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1817
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1929
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2057
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2216
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2487
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2685
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2786
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2787..2988
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3078
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3079..3251
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..3330
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3489
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3490..3636
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3637..3729
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3730..3844
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3845..4421
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4392..4397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /note="hexamer: AATAAA"
     polyA_site      4421
                     /gene="ADCY8"
                     /gene_synonym="AC8; ADCY3; HBAC1"
                     /note="major polyA site"
ORIGIN      
        1 atcccggcgc aatggagttc tccgaagggc gatgattcca gccacatctg ctaacttcgc
       61 acccatcgct gccgccggtc accgccggcc aggccccctg cagccgcgga gcagtgggcg
      121 tccaaagccc agtgcagcag ccaggacccg cccgacgcgc agcagaagca cggcgcccag
      181 gcgcttaggc gtctcttgga gagcaaaggc tgcgccaaaa cgctgagcct agaatcaacc
      241 aaggagcctg agcccaggaa ggggctgcgt ggctcacagc gctgcggctc ctgaggacaa
      301 atagccactg ccgctgcgta cccaagctgc gccggctggc gggagagcag cacgcaagga
      361 cgccgaggtc cgccgcgatc ttccaggtgc cctttgcccc tgggcacagt atgacccgac
      421 ctacagggag ccctagcgca gggctcctgc aacggggcag cctaggataa aaaggatcct
      481 tgccaagctc ctaccaggcc gcctttgagt ctttaggaac ccctcctccg gctgcctccc
      541 caaggttctg ggcctccttc cctgcggccc agagccatgg agctctccga tgtgcgctgc
      601 cttacaggca gcgaggaact ctacaccatc cacccgacgc ccccggccgg cgacggcagg
      661 agcgcctccc ggccgcagcg gctgctgtgg cagacggcgg tgcgacacat cacggagcag
      721 cgcttcattc acgggcaccg gggaggcagc ggcagcggga gtggaggctc gggcaaagcc
      781 tcggaccctg cgggcggcgg ccccaaccac cacgcgccgc agctgtcagg cgactcggcg
      841 ctgcccctct actcgctggg cccgggagag cgagcgcaca gcacctgcgg caccaaagtc
      901 ttcccggaac gcagcgggag cggcagtgcc agcggcagcg gaggcggggg cgacctgggc
      961 ttcctgcacc ttgactgtgc ccctagcaac tcggatttct ttcttaatgg gggctatagc
     1021 taccgagggg tcattttccc caccctgcgc aactccttca aatctcggga tttggaacgc
     1081 ctctaccagc gctatttctt gggccaaagg cgcaaatcgg aagtggtgat gaacgtgctg
     1141 gacgtgctga ccaaactcac tctcttggtc ctacacttga gcctggcctc ggcccccatg
     1201 gacccgctca agggcatcct gctgggcttc ttcaccggca ttgaggtagt gatctgcgcc
     1261 ctggtggtgg tcaggaagga caccacctcc cacacgtacc tgcagtacag cggcgtggtc
     1321 acctgggtgg ccatgaccac ccagatcctg gcagcaggcc tcggctacgg gctcctgggc
     1381 gacggcatag gctacgtgct cttcacgctc ttcgccacct acagtatgct gccgctgccg
     1441 ctcacctggg ccatcctggc cggcctgggc acctcgctgc tgcaggtcat cctccaagtg
     1501 gtcatacccc ggctggcggt catttccatc aaccaggttg tggcccaggc agtgctattc
     1561 atgtgtatga acacagctgg aatcttcatc agttacctgt cagaccgggc ccagcgccaa
     1621 gctttcctgg agactcggag gtgtgtggag gccaggctgc gcctggagac agagaaccaa
     1681 agacaggagc ggctcgtgct ttctgtgctc ccccggtttg ttgtcctgga aatgatcaac
     1741 gacatgacca atgtggaaga tgagcacctg cagcaccagt tccatcggat ctacatccat
     1801 cgctatgaga acgtcagtat tctttttgca gatgttaaag gatttaccaa cctctccacg
     1861 accttgtctg ctcaggagct ggtcaggatg ctcaacgagc tctttgccag atttgatcga
     1921 ctggcccatg agcatcactg ccttcgtatt aaaatcctgg gggactgcta ctactgcgtg
     1981 tctggacttc ctgagccccg ccaggaccat gcccactgct gtgttgaaat gggtctcagc
     2041 atgatcaaaa ccatcaggta tgtgcggtca aggacaaaac acgatgttga catgaggatt
     2101 ggaatccact ccggctcggt gctgtgcggt gttttgggac taaggaagtg gcagtttgat
     2161 gtctggtctt gggatgtgga tattgcaaac aaactcgaat ctggaggaat ccctgggagg
     2221 attcacattt ccaaagccac gctggactgt ctcaacggtg actataacgt ggaagagggc
     2281 catggtaaag agaggaatga attcctgagg aagcataata tcgaaactta cttaattaag
     2341 cagcctgagg acagtctgct gtccttgcct gaagatatcg tcaaggagtc agtgagctcc
     2401 tcagaccgga gaaacagtgg ggccacattc actgaaggat cctggagccc tgaactgccc
     2461 tttgataata tcgtggggaa acagaatact ctggctgccc taacaagaaa ttcaataaat
     2521 ctgcttccaa accatcttgc acaagctttg catgtccagt ctgggcctga ggaaattaac
     2581 aagagaatag aacataccat cgacttgcgg agtggcgata aattgagaag agagcatatc
     2641 aagccattct cactgatgtt taaagactcc agcctggagc acaagtattc tcaaatgagg
     2701 gatgaagtgt tcaagtcaaa cttggtctgt gcatttatcg ttcttctatt tatcacggca
     2761 atacaaagtt tgcttccttc ttcaagagtg atgccaatga ccatccagtt ctccattctg
     2821 attatgctgc actcggctct ggtcctcatc accacagcag aggattataa atgtttgccc
     2881 ctcatcctcc ggaaaacttg ctgttggatt aatgagacct atttggcccg gaacgtcatc
     2941 atctttgcat ccattttgat taatttcctg ggtgccatct taaatatcct gtggtgtgat
     3001 tttgacaagt cgataccctt gaagaacctg actttcaatt cctcagctgt gtttacagat
     3061 atctgctcct acccagagta ctttgtcttc acgggggtgt tggccatggt gacctgtgca
     3121 gttttcctcc ggctgaactc cgtcctgaag ctggcagtgc tgctgatcat gattgccatc
     3181 tatgccctgc tcactgagac cgtctacgca ggcctctttc tgcgttatga caacctcaac
     3241 cacagtggag aagatttcct ggggaccaag gaggtatcac tgctactgat ggccatgttc
     3301 ctcctggctg tgttctacca tggacagcag ctggagtaca cagcccgcct ggacttcctt
     3361 tggcgagtac aggccaaaga ggagatcaat gagatgaagg agctgaggga acacaatgag
     3421 aacatgctcc ggaatatctt acccagccat gtggcccgcc atttcctaga gaaggaccga
     3481 gacaatgagg agctgtattc tcaatcctat gatgctgttg gggtgatgtt tgcctccatc
     3541 ccaggatttg cggactttta ctctcagact gaaatgaata accagggagt ggaatgcctg
     3601 cgcttgctca atgagatcat tgctgacttc gatgagttgc ttggtgaaga ccgatttcaa
     3661 gacattgaaa agattaagac cattggcagc acctacatgg ccgtgtcagg cctgtcacct
     3721 gaaaaacagc aatgtgaaga caagtgggga catttgtgtg ctctggctga cttctcactc
     3781 gccctgacag aaagcataca ggagatcaac aagcattcat tcaacaattt tgaactccgg
     3841 attggcatca gccacggctc agtggtagct ggcgttatcg gcgctaagaa accacagtat
     3901 gacatttggg gcaaaactgt gaacctggca agccgaatgg acagcacggg ggttagtggc
     3961 cggatccaag tcccagagga gacctatctc atcctgaagg accagggctt tgcctttgat
     4021 taccgagggg agatctatgt gaagggtatc agtgaacagg aaggaaaaat caaaacgtac
     4081 tttcttctgg gaagagtcca acccaaccca ttcatcttgc ccccaagaag actgcctggg
     4141 cagtactccc tggccgcggt tgtcctggga cttgtccagt ccctcaatag gcaaaggcag
     4201 aagcagctac tcaatgagaa caacaacaca ggaatcatca agggtcatta caaccggcgg
     4261 actttgttgt cacccagcgg cacagagcct ggagcccagg ctgaaggcac cgacaaatct
     4321 gatttgccat aaaagcattt tctttctgtt tttttttttt gtatttcttt tatatataaa
     4381 ataaatatac taataaaaag gtttaatttt ttttagaaca a
//
