LOCUS       NM_007038               9621 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif
            5 (ADAMTS5), mRNA.
ACCESSION   NM_007038
VERSION     NM_007038.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Gao,S., Jia,M., Wang,J., Sun,Q., Liu,F., Yu,L., Guo,Y., Li,N. and
            Wei,L.
  TITLE     Association of ADAMTS-5 gene polymorphisms with the susceptibility
            to knee osteoarthritis in a Chinese Han population
  JOURNAL   J Orthop Surg Res 19 (1), 513 (2024)
   PUBMED   39192347
  REMARK    GeneRIF: Association of ADAMTS-5 gene polymorphisms with the
            susceptibility to knee osteoarthritis in a Chinese Han population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Hao,X. and Wu,X.
  TITLE     SP1-mediated ADAMTS5 transcription promotes IL-1beta-induced
            chondrocyte injury via Wnt/beta-catenin pathway in osteoarthritis
  JOURNAL   Mol Med Rep 30 (2) (2024)
   PUBMED   38940327
  REMARK    GeneRIF: SP1-mediated ADAMTS5 transcription promotes
            IL-1beta-induced chondrocyte injury via Wnt/beta-catenin pathway in
            osteoarthritis.
REFERENCE   3  (bases 1 to 9621)
  AUTHORS   Chen,Z., Du,F., Zhang,R., Wu,Q., Lu,Z., Zhang,R.L. and Wang,Q.
  TITLE     ADAMTS5 Promotes Permeability of the Blood-Brain Barrier during
            Treponema pallidum Subspecies pallidum Invading the Central Nervous
            System
  JOURNAL   ACS Infect Dis 10 (4), 1222-1231 (2024)
   PUBMED   38536197
  REMARK    GeneRIF: ADAMTS5 Promotes Permeability of the Blood-Brain Barrier
            during Treponema pallidum Subspecies pallidum Invading the Central
            Nervous System.
REFERENCE   4  (bases 1 to 9621)
  AUTHORS   Su,J.Q., Lai,P.Y., Hu,P.H., Hu,J.M., Chang,P.K., Chen,C.Y.,
            Wu,J.J., Lin,Y.J., Sun,C.A., Yang,T., Hsu,C.H., Lin,H.C. and
            Chou,Y.C.
  TITLE     Differential DNA methylation analysis of SUMF2, ADAMTS5, and PXDN
            provides novel insights into colorectal cancer prognosis prediction
            in Taiwan
  JOURNAL   World J Gastroenterol 28 (8), 825-839 (2022)
   PUBMED   35317099
  REMARK    GeneRIF: Differential DNA methylation analysis of SUMF2, ADAMTS5,
            and PXDN provides novel insights into colorectal cancer prognosis
            prediction in Taiwan.
REFERENCE   5  (bases 1 to 9621)
  AUTHORS   He,S., Li,J., Ma,D., Liu,Z. and Lv,N.
  TITLE     MicroRNA-148a targets ADAMTS5 to inhibit proliferation of
            endometriosis cells
  JOURNAL   Pak J Pharm Sci 35 (1(Special)), 335-341 (2022)
   PUBMED   35236644
  REMARK    GeneRIF: MicroRNA-148a targets ADAMTS5 to inhibit proliferation of
            endometriosis cells.
REFERENCE   6  (bases 1 to 9621)
  AUTHORS   Tang,B.L.
  TITLE     ADAMTS: a novel family of extracellular matrix proteases
  JOURNAL   Int J Biochem Cell Biol 33 (1), 33-44 (2001)
   PUBMED   11167130
  REMARK    Review article
REFERENCE   7  (bases 1 to 9621)
  AUTHORS   Mercuri,F.A., Maciewicz,R.A., Tart,J., Last,K. and Fosang,A.J.
  TITLE     Mutations in the interglobular domain of aggrecan alter matrix
            metalloproteinase and aggrecanase cleavage patterns. Evidence that
            matrix metalloproteinase cleavage interferes with aggrecanase
            activity
  JOURNAL   J Biol Chem 275 (42), 33038-33045 (2000)
   PUBMED   11032846
REFERENCE   8  (bases 1 to 9621)
  AUTHORS   Hurskainen,T.L., Hirohata,S., Seldin,M.F. and Apte,S.S.
  TITLE     ADAM-TS5, ADAM-TS6, and ADAM-TS7, novel members of a new family of
            zinc metalloproteases. General features and genomic distribution of
            the ADAM-TS family
  JOURNAL   J Biol Chem 274 (36), 25555-25563 (1999)
   PUBMED   10464288
REFERENCE   9  (bases 1 to 9621)
  AUTHORS   Abbaszade,I., Liu,R.Q., Yang,F., Rosenfeld,S.A., Ross,O.H.,
            Link,J.R., Ellis,D.M., Tortorella,M.D., Pratta,M.A., Hollis,J.M.,
            Wynn,R., Duke,J.L., George,H.J., Hillman,M.C. Jr., Murphy,K.,
            Wiswall,B.H., Copeland,R.A., Decicco,C.P., Bruckner,R., Nagase,H.,
            Itoh,Y., Newton,R.C., Magolda,R.L., Trzaskos,J.M., Burn,T.C. et al.
  TITLE     Cloning and characterization of ADAMTS11, an aggrecanase from the
            ADAMTS family
  JOURNAL   J Biol Chem 274 (33), 23443-23450 (1999)
   PUBMED   10438522
REFERENCE   10 (bases 1 to 9621)
  AUTHORS   Fosang,A.J., Last,K., Fujii,Y., Seiki,M. and Okada,Y.
  TITLE     Membrane-type 1 MMP (MMP-14) cleaves at three sites in the aggrecan
            interglobular domain
  JOURNAL   FEBS Lett 430 (3), 186-190 (1998)
   PUBMED   9688535
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK094057.1, AP001601.1,
            AF142099.1, AP001600.1 and CA313018.1.
            
            On Nov 22, 2018 this sequence version replaced NM_007038.4.
            
            Summary: This gene encodes a member of the ADAMTS (a disintegrin
            and metalloproteinase with thrombospondin motifs) protein family.
            Members of the family share several distinct protein modules,
            including a propeptide region, a metalloproteinase domain, a
            disintegrin-like domain, and a thrombospondin type 1 (TS) motif.
            Individual members of this family differ in the number of
            C-terminal TS motifs, and some have unique C-terminal domains. The
            encoded preproprotein is proteolytically processed to generate the
            mature enzyme. This enzyme contains two C-terminal TS motifs and
            functions as an aggrecanase that cleaves aggrecan, a major
            proteoglycan of cartilage, and may mediate cartilage destruction in
            osteoarthritis. [provided by RefSeq, Feb 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF142099.1, SRR14038195.1319587.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000284987.6/ ENSP00000284987.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-431               AK094057.1         33-463
            432-1110            AP001601.1         30235-30913         c
            1111-2771           AF142099.1         536-2196
            2772-2772           AP001600.1         79167-79167         c
            2773-3537           AF142099.1         2198-2962
            3538-9157           AP001600.1         67517-73136         c
            9158-9621           CA313018.1         28-491              c
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q21.3"
     gene            1..9621
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /note="ADAM metallopeptidase with thrombospondin type 1
                     motif 5"
                     /db_xref="GeneID:11096"
                     /db_xref="HGNC:HGNC:221"
                     /db_xref="MIM:605007"
     exon            1..1801
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             698..3490
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /note="aggrecanase-2; a disintegrin-like and
                     metalloprotease (reprolysin type) with thrombospondin type
                     1 motif, 5 (aggrecanase-2); a disintegrin and
                     metalloproteinase with thrombospondin motifs 5; a
                     disintegrin and metalloproteinase with thrombospondin
                     motifs 11"
                     /codon_start=1
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 5 preproprotein"
                     /protein_id="NP_008969.2"
                     /db_xref="CCDS:CCDS13579.1"
                     /db_xref="GeneID:11096"
                     /db_xref="HGNC:HGNC:221"
                     /db_xref="MIM:605007"
                     /translation="MLLGWASLLLCAFRLPLAAVGPAATPAQDKAGQPPTAAAAAQPR
                     RRQGEEVQERAEPPGHPHPLAQRRRSKGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDL
                     ERDGSVGIAGFVPAGGGTSAPWRHRSHCFYRGTVDGSPRSLAVFDLCGGLDGFFAVKH
                     ARYTLKPLLRGPWAEEEKGRVYGDGSARILHVYTREGFSFEALPPRASCETPASTPEA
                     HEHAPAHSNPSGRAALASQLLDQSALSPAGGSGPQTWWRRRRRSISRARQVELLLVAD
                     ASMARLYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLGDKDKSLEVSKNA
                     ATTLKNFCKWQHQHNQLGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPER
                     SCAVIEDDGLHAAFTVAHEIGHLLGLSHDDSKFCEETFGSTEDKRLMSSILTSIDASK
                     PWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQTYDATQQCNLTFGPEYSVC
                     PGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRICLQGKCVDKTKKKYYSTSS
                     HGNWGSWGSWGQCSRSCGGGVQFAYRHCNNPAPRNNGRYCTGKRAIYRSCSLMPCPPN
                     GKSFRHEQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSP
                     KVTDGTECRLYSNSVCVRGKCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIVGTFNK
                     KSKGYTDVVRIPEGATHIKVRQFKAKDQTRFTAYLALKKKNGEYLINGKYMISTSETI
                     IDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPTKPLDVRYSFFVPKKST
                     PKVNSVTSHGSNKVGSHTSQPQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLAKGCP
                     LSQRPSAFKQCLLKKC"
     exon            1802..1934
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1935..2102
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2386
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2570
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2746
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2922
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2923..9621
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9600..9605
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /note="hexamer: AATAAA"
     polyA_site      9621
                     /gene="ADAMTS5"
                     /gene_synonym="ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11;
                     ADAMTS-5; ADAMTS11; ADMP-2"
                     /note="major polyA site"
ORIGIN      
        1 acagcgctcg cgctgctctc ggcgctcgca gctgccgact ggggatgacg gcgggcagga
       61 ggagaccgca gccgaaggga cacagacacg ccgcttcacc agctcgcctc aggctgcccc
      121 cctgcatttt tgttttaatt tttacggctt tttcccctct ctttcttccc ttcctcctgg
      181 tcccagcaga gccaaggaaa cccacaaaat aagaaaggaa gtgggccccg gagcttggaa
      241 cctccacagc cggcttgtcc agcgcagcgc gggggcggga ggctgcgcgc accagttgcc
      301 agcccggtgc gcggtacctt tccttacttt tcttgaaaca gcgatcgtgc ctgcatttgg
      361 tggttttttg gtttttgttt ttttcctttt cccgtatttg ctgaatctcc actatccgac
      421 tttttttttt taatcttttc tttccccccc cccccacccc acctctttct ggagcacgaa
      481 tccaaacatt ttcccaagca acaaagaaaa gttcgcacgc tggcaccgca gcccggacag
      541 gctggcgctg ctgccgggcc cccctccctc cgacacttga ctcaatcctg caagcaagtg
      601 tgtgtgtgtc cccatccccc gccccgttaa cttcatagca aataacaaat acccataaag
      661 tcccagtcgc gcagcccctc cccgcgggca gcgcactatg ctgctcgggt gggcgtccct
      721 gctgctgtgc gcgttccgcc tgcccctggc cgcggtcggc cccgccgcga cacctgccca
      781 ggataaagcc gggcagcctc cgactgctgc agcagccgcc cagccccgcc ggcggcaggg
      841 ggaggaggtg caggagcgag ccgagcctcc cggccacccg caccccctgg cgcagcggcg
      901 caggagcaag gggctggtgc agaacatcga ccaactctac tccggcggcg gcaaggtggg
      961 ctacctcgtc tacgcgggcg gccggaggtt cctcttggac ctggagcgag atggttcggt
     1021 gggcattgct ggcttcgtgc ccgcaggagg cgggacgagt gcgccctggc gccaccggag
     1081 ccactgcttc tatcggggca cagtggacgg tagtccccgc tctctggctg tctttgacct
     1141 ctgtgggggt ctcgacggct tcttcgcggt caagcacgcg cgctacaccc taaagccact
     1201 gctgcgcgga ccctgggcgg aggaagaaaa ggggcgcgtg tacggggatg ggtccgcacg
     1261 gatcctgcac gtctacaccc gcgagggctt cagcttcgag gccctgccgc cgcgcgccag
     1321 ctgcgaaacc cccgcgtcca caccggaggc ccacgagcat gctccggcgc acagcaaccc
     1381 gagcggacgc gcagcactgg cctcgcagct cttggaccag tccgctctct cgcccgctgg
     1441 gggctcagga ccgcagacgt ggtggcggcg gcggcgccgc tccatctccc gggcccgcca
     1501 ggtggagctg cttctggtgg ctgacgcgtc catggcgcgg ttgtatggcc ggggcctgca
     1561 gcattacctg ctgaccctgg cctccatcgc caataggctg tacagccatg ctagcatcga
     1621 gaaccacatc cgcctggccg tggtgaaggt ggtggtgcta ggcgacaagg acaagagcct
     1681 ggaagtgagc aagaacgctg ccaccacact caagaacttt tgcaagtggc agcaccaaca
     1741 caaccagctg ggagatgacc atgaggagca ctacgatgca gctatcctgt ttactcggga
     1801 ggatttatgt gggcatcatt catgtgacac cctgggaatg gcagacgttg ggaccatatg
     1861 ttctccagag cgcagctgtg ctgtgattga agacgatggc ctccacgcag ccttcactgt
     1921 ggctcacgaa atcggacatt tacttggcct ctcccatgac gattccaaat tctgtgaaga
     1981 gacctttggt tccacagaag ataagcgctt aatgtcttcc atccttacca gcattgatgc
     2041 atctaagccc tggtccaaat gcacttcagc caccatcaca gaattcctgg atgatggcca
     2101 tggtaactgt ttgctggacc taccacgaaa gcagatcctg ggccccgaag aactcccagg
     2161 acagacctac gatgccaccc agcagtgcaa cctgacattc gggcctgagt actccgtgtg
     2221 tcccggcatg gatgtctgtg ctcgcctgtg gtgtgctgtg gtacgccagg gccagatggt
     2281 ctgtctgacc aagaagctgc ctgcggtgga agggacgcct tgtggaaagg ggagaatctg
     2341 cctgcagggc aaatgtgtgg acaaaaccaa gaaaaaatat tattcaacgt caagccatgg
     2401 caactgggga tcttggggat cctggggcca gtgttctcgc tcatgtggag gaggagtgca
     2461 gtttgcctat cgtcactgta ataaccctgc tcccagaaac aacggacgct actgcacagg
     2521 gaagagggcc atctaccgct cctgcagtct catgccctgc ccacccaatg gtaaatcatt
     2581 tcgtcatgaa cagtgtgagg ccaaaaatgg ctatcagtct gatgcaaaag gagtcaaaac
     2641 ttttgtggaa tgggttccca aatatgcagg tgtcctgcca gcggatgtgt gcaagctgac
     2701 ctgcagagcc aagggcactg gctactatgt ggtattttct ccaaaggtga ccgatggcac
     2761 tgaatgtagg ctgtacagta attccgtctg cgtccggggg aagtgtgtga gaactggctg
     2821 tgacggcatc attggctcaa agctgcagta tgacaagtgc ggagtatgtg gaggagacaa
     2881 ctccagctgt acaaagattg ttggaacctt taataagaaa agtaagggtt acactgacgt
     2941 ggtgaggatt cctgaagggg caacccacat aaaagttcga cagttcaaag ccaaagacca
     3001 gactagattc actgcctatt tagccctgaa aaagaaaaac ggtgagtacc ttatcaatgg
     3061 aaagtacatg atctccactt cagagactat cattgacatc aatggaacag tcatgaacta
     3121 tagcggttgg agccacaggg atgacttcct gcatggcatg ggctactctg ccacgaagga
     3181 aattctaata gtgcagattc ttgcaacaga ccccactaaa ccattagatg tccgttatag
     3241 cttttttgtt cccaagaagt ccactccaaa agtaaactct gtcactagtc atggcagcaa
     3301 taaagtggga tcacacactt cgcagccgca gtgggtcacg ggcccatggc tcgcctgctc
     3361 taggacctgt gacacaggtt ggcacaccag aacggtgcag tgccaggatg gaaaccggaa
     3421 gttagcaaaa ggatgtcctc tctcccaaag gccttctgcg tttaagcaat gcttgttgaa
     3481 gaaatgttag cctgtggtta tgatcttatg cacaaagata actggaggat tcagcactga
     3541 tgcagtcgtg gtgaacagga ggtctaccta acgcacagaa agtcatgctt cagtgacatt
     3601 gtcaacagga gtccaattat gggcagaatc tgctctctgt gaccaaaaga ggatgtgcac
     3661 tgcttcacgt gacagtggtg accttgcaat atagaaaaac ttgggagtta ttgaacatcc
     3721 cctgggctta caagaaacac tgatgaatgt aaaatcaggg gacatttgaa gatggcagaa
     3781 ctgtctcccc cttgtcacct acctctgata gaatgtcttt aatggtatca taatcatttt
     3841 cacccataat acacagtagc ttcttcttac tgtttgtaaa tacattctcc cttggtatgt
     3901 cactttatat cccctggttc tattaaaata tccatatata tttctataaa aaaagtgttt
     3961 gaccaaagta ggtctgcagc tatttcaact tccttccgtt tccagaaaga gctgtggata
     4021 ttttactgga aattaagaac ttgctgctgt tttaataaga tgtagtatat tttctgacta
     4081 caggagataa aatttcagtc aaaaaaccat tttgacagca agtatcttct gagaaatttt
     4141 gaaaagtaaa tagatctcag tgtatctagt cacttaaata catacacggg ttcatttact
     4201 taaacctttg actgcctgta tttttttcag gtagctagcc aaattaatgc ataatttcag
     4261 atgtagaagt agggtttgcg tgtgtgtgtg tgatcatact caagagtcta aaaactagtt
     4321 tccttgtgtt ggaaatttaa aaggaaaaaa atcgtatttc actgtgtttt caatttatat
     4381 tttcacaact actttctctc tccagagctt tcatctgata tctcacaatg tatgatatac
     4441 gtacaaaaca cacagcaagt tttctatcat gtccaacaca ttcaacactg gtatacctcc
     4501 taccagcaag cctttaaaat gcatttgtgt ttgcttattt gttttgttca agggttcagt
     4561 aagacctaca atgttttgta tttcttgact tattttatta gaaacattaa agatcacttg
     4621 gtagttagcc acattgagaa gtggttatca ttgttaatgt ggttaatgcc aaaaagtggt
     4681 taatattaat aagactgttt ccacaccata ggcaataatt tcttaattta aaaaatctaa
     4741 gtatattcct attgtactaa atatttttcc caactggaaa gcacttgatt gtacccgtaa
     4801 gtgtttgagt gatgacatgt gatgattttc agaaagttgt tgtttttgtt tccatagcct
     4861 gtttaagtag gttgtaagtt tgaatagtta gacatggaaa ttattttata agcacacacc
     4921 taaagatatc tttttagatg ataaaatgta caccccccca tcaccaacct cacaacttag
     4981 aaaatctaag ttgtttgatt tctttgggat ttcttttgtt gtgaaacact gcaaagccaa
     5041 tttttcttta taaaaattca tagtaatcct gccaaatgtg cctattgtta aagatttgca
     5101 tgtgaagatc ttagggaacc actgtttgag ttctacaagc tcatgagagt ttatttttat
     5161 tataagatgt ttttaatata aaagaattat gtaactgatc actatattac atcatttcag
     5221 tgggccagga aaatagatgt cttgctgttt tcagtatttt cttaagaaat tgcttttaaa
     5281 acaaataatt gttttacaaa accaataatt atcctttgaa ttttcataga ctgactttgc
     5341 ttttgacgta gaaatttttt ttctcaataa attatcactt tgagaaatga ggcctgtaca
     5401 aggctgataa cctatatgtg atggagatca cccaatgcca agggcagaaa gcaaacctag
     5461 ttaaataggt gagaaaaaaa ataataatcc cagtgccatt tgtctgtgca aagagaatta
     5521 ggagagaggt taatgttact tttttccatt ttggaaataa ttttaatcaa gtaactcaaa
     5581 tgtgacaaaa tttattttta ttttttgtgg ttatattccc aacaacatta aaaaatactc
     5641 gaggcataaa tgtagttgtc tcctactctg cttctcttac tatactcata catttttaat
     5701 atggtttatc aatgattcat gtttccctca aatagtgatg gtttacacct gtcatggaaa
     5761 caatcctaga gagctcagag caattaaacc actattccat gcttttaagt agttttctcc
     5821 acctttttct tatgagtctc actagattga ctgaggaatg tatgtctaaa ttcctggaga
     5881 agatgatatg gattggaaac tgaaattcag agaaatggag tgttcaatag ataccacgaa
     5941 ttgtgaacaa agggaaaatt ctatacaact caatctaagt cagtccactt tgacttcgta
     6001 ctgtctttca cctttccatt gttgcatctt gaatttttta aaatgtctag aattcaggat
     6061 gctaggggct acttctttaa aaaaaaaaaa aaaaaagaat tcgtctgaaa atgctcaggt
     6121 ttgtaagaat ctaatctcac ttacataact aagcactcca taataagttt tattaagtac
     6181 aaagggagcc agaaaaaatg acatttattt cttctagatc agaaaaattt aaattaagcc
     6241 ctgccttgct gtttagaaat atgtgggcat tgttataatt tattcaataa atttatgttc
     6301 ctttgccttc ctgtggaaac agttttatcc cactaaacta ggaattaggg gataaatcac
     6361 aaacaaaaaa aaagttgcag cactgaaaaa aagtaattta ttgtttttgc aactggtatg
     6421 tgaatttgtg tgataaaatt atttattctt atttaacaaa aatatgttca aatttttcta
     6481 tatttaaaat gttttgctgt tgtcctactt tttaatttat gcttcatgtt tgtgtataaa
     6541 gtacactttt acactttgtg agtttacata atatacagca ctggttgctt ttgtattttt
     6601 ttacagaaag ctttctgtgt gaagcaggtg tatatgtata tattcctcat gtattcttat
     6661 tctgatacta tcatttttct ttccaaggaa attttaatct gtcatgacca atagtgttca
     6721 ttacttgtgc ctatgataat aggtttttta catcacatta acactatttt ttccaagtca
     6781 caaataagaa aaacacttat tcaatgaaac aaggtgcaag ttttaaattt gggtacacaa
     6841 atagcctaga agcttcctac agacgctaag acacagccaa taatcagatc ctttcacttc
     6901 atcgagaaac ttggacaagt cgatattgat gtattagatg aaagttgtct acacacaact
     6961 tctgagggat acaaacgata ataaaaccaa atgttgtctg tttctccttt agaaacacct
     7021 cctaaaatta atatcattta gtctctagtg tctgtaggat tctacagatg agcacaaata
     7081 gattgggttt gtataacaaa tgctaatagt cataactgtt tctacaaata tggggtgtcc
     7141 attaagagaa tgtgatgttt tcctactgct gttgaatccc atggggtgat tataggactt
     7201 gaaataggca gagtcacctc tgatgacatc agcttgcctc tgtgatttca cagtctgatc
     7261 ctggcaacaa gacaaagcac ccttggacac acagccaatc tctggttgtg atatttcccc
     7321 attgattcct tccttgttaa caaggtcatt ttaatggttc aggtgaggac agcagccaga
     7381 ttcaaagtcc agaatttgtg ctgttacata gagttcacac tgtcaaataa cattgaattt
     7441 aataatgatc aaatttttct agtagtcttt ggcagagtgt ataatctcat tggcatgatt
     7501 ggtgaatatt actaatctct ttataatgaa agatgcttta caaatacctt atatttgcta
     7561 acatttcaaa actactaaat aaatgaaata gccatgtgta cagaaatggt catttaaagc
     7621 tttaatagaa ccaaattcaa gacaatgtat catttagaca cacagaaaag gaacttgtat
     7681 gttttcccta ttatttttct catttgccaa caatctatag ttttaggtta tcaaacagat
     7741 agatcaactt aactggctag tacattgaaa aatcttccta agaatccttt gttagcataa
     7801 tctatagaga taatttctca aattatatca tcatgatgca tataaactct ataatgtata
     7861 attgtgtttc atttatttaa tgtatgagaa catattgaaa tacaaaacca tgcattagcc
     7921 aaaaaattgg aatacaggta gtgttcagat cagcaaaaca ttcagtctgg taaatgcctg
     7981 cctggggcta tgatatcatt ctcaatgcag gttttatgga aaaactaaaa gaatatgttg
     8041 ttagatgatg ttggttttga aaaaaaaaag acattaacat acacattagt tagcccagtt
     8101 aattgcattc tactaatata gttgcacatt agcaataatt ttgctgtctc tggtctttat
     8161 tttgtggctt caactaactg gaccatgtgg actgtaaagg tcaaatggaa aaaacgagca
     8221 gtggcccctc atcctgtaag gtactgctac atcagagtga cctaaaagtc taacactgtg
     8281 aggaaaactg tgatttgtag gaaaaaaaaa aaaaacaaat aaaaaacagg gcatgctttt
     8341 taattttttt ccactttcct ttggcacacc caatgaacaa ttctaatttt tattgaggtg
     8401 ctaacatctt tcgtgaccga ctgtcaaatg tggtattttt gagttactat ttttctacat
     8461 gattttacag tttgcaagaa agacctctaa gctttgtgtc acggtagggc acaacttgat
     8521 actcaaaatt tgaaaaataa gcacatccaa tgattgtttt gaccaacagt ggtcagtgac
     8581 gtaaactgca tgtgcatctg aggacattta aggggtcatt aaaatttgag gagcatcagg
     8641 ccggagtagc agacttttag atgagtcata tttcagcatt cactaagtcc tcagcattcc
     8701 attcaaactg tcgtgtatat ttggcctgat tttttttcaa gctttgcaat aatttatgtt
     8761 attggtaaac acttggtgac tatatctcag ccttttcttt aacaactcac aatatattag
     8821 aaacacgtct acctatactg agagtatatt tacaatagaa gaacatactg tatgtgactt
     8881 tgtaaagcta gacttttgat taagaaatat ataatctctg gatgctattt ttgcattata
     8941 cactcaggca caacgtaaac cttgatggct catcttgcta caattacgag ttgaaaaaca
     9001 ctacttacgt atttgtatga cctattagtc agaggaaatc atacatatgc tttgtaaata
     9061 gactttgcag ataactaaat agactgaaga aatatgttgc atttgataga agcaattgca
     9121 taaatatttg gtttctatat tagagtctgt gagtaaagtc aagtaataaa cctaagtagg
     9181 tataacagat ttttaaacct tgaaacttgc tttgatggta gagaaaatca ttgaagattt
     9241 acatactgta tataagatgt aaaatgtacg ctgcttatta ccctcaattt tccagaagca
     9301 atggtatata atgcagttga aaaaccaaaa atcttggaaa actaagacgg gtcttgttta
     9361 aaatgtctct cagctttggc aaccttcaaa tcttaatcaa ctatttaaag cattactgtg
     9421 tcttgtagcc tgcattccac aacagctctg ttattcaggt aaaagacttg aactgagccg
     9481 tttgggacct atactgtaat attttcattg aggaacaata tcctattttg taaagcattt
     9541 ccctatgtgt gactttaaac tgtaaaatta aacactgctt ttgtgggttc agtgggcata
     9601 ataaatataa attgtaaact a
//
