LOCUS       NM_030955               8572 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif
            12 (ADAMTS12), transcript variant 1, mRNA.
ACCESSION   NM_030955
VERSION     NM_030955.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Hoeft,K., Koch,L., Ziegler,S., Zhang,L., Luetke,S., Tanzer,M.C.,
            Mohanta,D., Schumacher,D., Schreibing,F., Long,Q., Kim,H.,
            Klinkhammer,B.M., Schikarski,C., Maryam,S., Baens,M., Hermann,J.,
            Krieg,S., Peisker,F., De Laporte,L., Schaefer,G.J., Menzel,S.,
            Jankowski,J., Humphreys,B.D., Wahida,A., Schneider,R.K.,
            Versele,M., Boor,P., Mann,M., Sengle,G., Hayat,S. and Kramann,R.
  TITLE     ADAMTS12 promotes fibrosis by restructuring extracellular matrix to
            enable activation of injury-responsive fibroblasts
  JOURNAL   J Clin Invest 134 (18), e170246 (2024)
   PUBMED   39286973
  REMARK    GeneRIF: ADAMTS12 promotes fibrosis by restructuring extracellular
            matrix to enable activation of injury-responsive fibroblasts.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Dekky,B., Azar,F., Bonnier,D., Monseur,C., Kalebic,C., Arpigny,E.,
            Colige,A., Legagneux,V. and Theret,N.
  TITLE     ADAMTS12 is a stromal modulator in chronic liver disease
  JOURNAL   FASEB J 37 (11), e23237 (2023)
   PUBMED   37819632
  REMARK    GeneRIF: ADAMTS12 is a stromal modulator in chronic liver disease.
REFERENCE   3  (bases 1 to 8572)
  AUTHORS   Jiang,Y., Huang,J., Huang,Z., Li,W., Tan,R., Li,T., Chen,Z.,
            Tang,X., Zhao,Y., Qiu,J., Li,C., Chen,H. and Yang,Z.
  TITLE     ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in
            gastric cancer through VEGF upregulation
  JOURNAL   Cell Signal 111, 110866 (2023)
   PUBMED   37619822
  REMARK    GeneRIF: ADAMTS12 promotes oxaliplatin chemoresistance and
            angiogenesis in gastric cancer through VEGF upregulation.
REFERENCE   4  (bases 1 to 8572)
  AUTHORS   He,R.Z., Zheng,J.H., Yao,H.F., Xu,D.P., Yang,M.W., Liu,D.J.,
            Sun,Y.W. and Huo,Y.M.
  TITLE     ADAMTS12 promotes migration and epithelial-mesenchymal transition
            and predicts poor prognosis for pancreatic cancer
  JOURNAL   Hepatobiliary Pancreat Dis Int 22 (2), 169-178 (2023)
   PUBMED   35508435
  REMARK    GeneRIF: ADAMTS12 promotes migration and epithelial-mesenchymal
            transition and predicts poor prognosis for pancreatic cancer.
REFERENCE   5  (bases 1 to 8572)
  AUTHORS   Liang,J.F., Li,P.H., Zhu,Y., Zheng,S.S., Liu,J.W. and Song,S.Q.
  TITLE     MicroRNA-186 suppresses cell proliferation and metastasis in
            bladder cancer
  JOURNAL   Afr Health Sci 22 (4), 56-63 (2022)
   PUBMED   37092087
  REMARK    GeneRIF: MicroRNA-186 suppresses cell proliferation and metastasis
            in bladder cancer.
REFERENCE   6  (bases 1 to 8572)
  AUTHORS   Luan,Y., Kong,L., Howell,D.R., Ilalov,K., Fajardo,M., Bai,X.H., Di
            Cesare,P.E., Goldring,M.B., Abramson,S.B. and Liu,C.J.
  TITLE     Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage
            oligomeric matrix protein by alpha-2-macroglobulin
  JOURNAL   Osteoarthritis Cartilage 16 (11), 1413-1420 (2008)
   PUBMED   18485748
REFERENCE   7  (bases 1 to 8572)
  AUTHORS   Llamazares,M., Obaya,A.J., Moncada-Pazos,A., Heljasvaara,R.,
            Espada,J., Lopez-Otin,C. and Cal,S.
  TITLE     The ADAMTS12 metalloproteinase exhibits anti-tumorigenic properties
            through modulation of the Ras-dependent ERK signalling pathway
  JOURNAL   J Cell Sci 120 (Pt 20), 3544-3552 (2007)
   PUBMED   17895370
  REMARK    GeneRIF: data suggest that ADAMTS12 confers tumour-protective
            functions upon cells that produce this proteolytic enzyme
REFERENCE   8  (bases 1 to 8572)
  AUTHORS   Liu,C.J., Kong,W., Xu,K., Luan,Y., Ilalov,K., Sehgal,B., Yu,S.,
            Howell,R.D. and Di Cesare,P.E.
  TITLE     ADAMTS-12 associates with and degrades cartilage oligomeric matrix
            protein
  JOURNAL   J Biol Chem 281 (23), 15800-15808 (2006)
   PUBMED   16611630
  REMARK    GeneRIF: ADAMTS-12 is a new COMP-interacting and -degrading enzyme
            and thus may play an important role in the COMP degradation in the
            initiation and progression of arthritis
REFERENCE   9  (bases 1 to 8572)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   10 (bases 1 to 8572)
  AUTHORS   Tang,B.L.
  TITLE     ADAMTS: a novel family of extracellular matrix proteases
  JOURNAL   Int J Biochem Cell Biol 33 (1), 33-44 (2001)
   PUBMED   11167130
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC058841.1, AY358745.1,
            AJ250725.1, AW938686.1, AC034232.8 and CA413137.1.
            
            On Nov 23, 2018 this sequence version replaced NM_030955.3.
            
            Summary: This gene encodes a member of the ADAMTS (a disintegrin
            and metalloproteinase with thrombospondin motifs) protein family.
            Members of the family share several distinct protein modules,
            including a propeptide region, a metalloproteinase domain, a
            disintegrin-like domain, and a thrombospondin type 1 (TS-1) motif.
            Individual members of this family differ in the number of
            C-terminal TS-1 motifs, and some have unique C-terminal domains.
            The enzyme encoded by this gene contains eight TS-1 motifs. It may
            play roles in pulmonary cells during fetal development or in tumor
            processes through its proteolytic activity or as a molecule
            potentially involved in regulation of cell adhesion. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ250725.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000504830.6/ ENSP00000422554.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-538               BC058841.1         34-571
            539-768             AY358745.1         524-753
            769-2603            AJ250725.1         961-2795
            2604-2813           AW938686.1         295-504
            2814-4139           AJ250725.1         3003-4328
            4140-4157           AC034232.8         21823-21840
            4158-4616           AJ250725.1         4347-4805
            4617-4619           AC034232.8         48013-48015
            4620-4926           AJ250725.1         4809-5115
            4927-7933           AC034232.8         55789-58795
            7934-8572           CA413137.1         18-656              c
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.3-p13.2"
     gene            1..8572
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /note="ADAM metallopeptidase with thrombospondin type 1
                     motif 12"
                     /db_xref="GeneID:81792"
                     /db_xref="HGNC:HGNC:14605"
                     /db_xref="MIM:606184"
     exon            1..261
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..4919
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; a disintegrin-like and metalloprotease
                     (reprolysin type) with thrombospondin type 1 motif, 12; a
                     disintegrin and metalloproteinase with thrombospondin
                     motifs 12; ADAM-TS12; ADAMTS-12; ADAM-TS 12"
                     /codon_start=1
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 12 isoform 1 preproprotein"
                     /protein_id="NP_112217.2"
                     /db_xref="CCDS:CCDS34140.1"
                     /db_xref="GeneID:81792"
                     /db_xref="HGNC:HGNC:14605"
                     /db_xref="MIM:606184"
                     /translation="MPCAQRSWLANLSVVAQLLNFGALCYGRQPQPGPVRFPDRRQEH
                     FIKGLPEYHVVGPVRVDASGHFLSYGLHYPITSSRRKRDLDGSEDWVYYRISHEEKDL
                     FFNLTVNQGFLSNSYIMEKRYGNLSHVKMMASSAPLCHLSGTVLQQGTRVGTAALSAC
                     HGLTGFFQLPHGDFFIEPVKKHPLVEGGYHPHIVYRRQKVPETKEPTCGLKDSVNISQ
                     KQELWREKWERHNLPSRSLSRRSISKERWVETLVVADTKMIEYHGSENVESYILTIMN
                     MVTGLFHNPSIGNAIHIVVVRLILLEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDL
                     NPVHHDVAVLLTRKDICAGFNRPCETLGLSHLSGMCQPHRSCNINEDSGLPLAFTIAH
                     ELGHSFGIQHDGKENDCEPVGRHPYIMSRQLQYDPTPLTWSKCSEEYITRFLDRGWGF
                     CLDDIPKKKGLKSKVIAPGVIYDVHHQCQLQYGPNATFCQEVENVCQTLWCSVKGFCR
                     SKLDAAADGTQCGEKKWCMAGKCITVGKKPESIPGGWGRWSPWSHCSRTCGAGVQSAE
                     RLCNNPEPKFGGKYCTGERKRYRLCNVHPCRSEAPTFRQMQCSEFDTVPYKNELYHWF
                     PIFNPAHPCELYCRPIDGQFSEKMLDAVIDGTPCFEGGNSRNVCINGICKMVGCDYEI
                     DSNATEDRCGVCLGDGSSCQTVRKMFKQKEGSGYVDIGLIPKGARDIRVMEIEGAGNF
                     LAIRSEDPEKYYLNGGFIIQWNGNYKLAGTVFQYDRKGDLEKLMATGPTNESVWIQLL
                     FQVTNPGIKYEYTIQKDGLDNDVEQQMYFWQYGHWTECSVTCGTGIRRQTAHCIKKGR
                     GMVKATFCDPETQPNGRQKKCHEKACPPRWWAGEWEACSATCGPHGEKKRTVLCIQTM
                     VSDEQALPPTDCQHLLKPKTLLSCNRDILCPSDWTVGNWSECSVSCGGGVRIRSVTCA
                     KNHDEPCDVTRKPNSRALCGLQQCPSSRRVLKPNKGTISNGKNPPTLKPVPPPTSRPR
                     MLTTPTGPESMSTSTPAISSPSPTTASKEGDLGGKQWQDSSTQPELSSRYLISTGSTS
                     QPILTSQSLSIQPSEENVSSSDTGPTSEGGLVATTTSGSGLSSSRNPITWPVTPFYNT
                     LTKGPEMEIHSGSGEEREQPEDKDESNPVIWTKIRVPGNDAPVESTEMPLAPPLTPDL
                     SRESWWPPFSTVMEGLLPSQRPTTSETGTPRVEGMVTEKPANTLLPLGGDHQPEPSGK
                     TANRNHLKLPNNMNQTKSSEPVLTEEDATSLITEGFLLNASNYKQLTNGHGSAHWIVG
                     NWSECSTTCGLGAYWRRVECSTQMDSDCAAIQRPDPAKRCHLRPCAGWKVGNWSKCSR
                     NCSGGFKIREIQCVDSRDHRNLRPFHCQFLAGIPPPLSMSCNPEPCEAWQVEPWSQCS
                     RSCGGGVQERGVFCPGGLCDWTKRPTSTMSCNEHLCCHWATGNWDLCSTSCGGGFQKR
                     TVQCVPSEGNKTEDQDQCLCDHKPRPPEFKKCNQQACKKSADLLCTKDKLSASFCQTL
                     KAMKKCSVPTVRAECCFSCPQTHITHTQRQRRQRLLQKSKEL"
     exon            262..623
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            624..768
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            769..965
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1049
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1174
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1324
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1468
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1613
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1706
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1852
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..2022
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2156
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2277
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2522
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2661
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2788
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2999
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            3000..4106
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            4107..4259
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            4260..4436
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4580
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            4581..4740
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     exon            4741..8572
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8555..8560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /note="hexamer: AATAAA"
     polyA_site      8572
                     /gene="ADAMTS12"
                     /gene_synonym="PRO4389"
                     /note="major polyA site"
ORIGIN      
        1 agctttgcag tcgctgcctt ctcgcgcctg accatgcacc cctgcatctt cctgctgggc
       61 cacaggcgag cgctttattt ctggagctga gggctaaaac ttttttgact tttcttctcc
      121 tcaacatctg aatcatgcca tgtgcccaga ggagctggct tgcaaacctt tccgtggtgg
      181 ctcagctcct taactttggg gcgctttgct atgggagaca gcctcagcca ggcccggttc
      241 gcttcccgga caggaggcaa gagcatttta tcaagggcct gccagaatac cacgtggtgg
      301 gtccagtccg agtagatgcc agtgggcatt ttttgtcata tggcttgcac tatcccatca
      361 cgagcagcag gaggaagaga gatttggatg gctcagagga ctgggtgtac tacagaattt
      421 ctcacgagga gaaggacctg ttttttaact tgacggtcaa tcaaggattt ctttccaata
      481 gctacatcat ggagaagaga tatgggaacc tctcccatgt taagatgatg gcttcctctg
      541 cccccctctg ccatctcagt ggcacggttc tacagcaggg caccagagtt gggacggcag
      601 ccctcagtgc ctgccatgga ctgactggat ttttccaact accacatgga gactttttca
      661 ttgaacccgt gaagaagcat ccactggttg agggagggta ccacccgcac atcgtttaca
      721 ggaggcagaa agttccagaa accaaggagc caacctgtgg attaaaggac agtgttaaca
      781 tctcccagaa gcaagagcta tggcgggaga agtgggagag gcacaacttg ccaagcagaa
      841 gcctctctcg gcgttccatc agcaaggaga gatgggtgga gacactggtg gtggccgaca
      901 caaagatgat tgaataccat gggagtgaga atgtggagtc ctacatcctc accatcatga
      961 acatggtcac tgggttgttc cataacccaa gcattggcaa tgcaattcac attgttgtgg
     1021 ttcggctcat tctactcgaa gaagaagagc aaggactgaa aatagttcac catgcagaaa
     1081 agacactgtc tagcttctgc aagtggcaga agagtatcaa tcccaagagt gacctcaatc
     1141 ctgttcatca cgacgtggct gtccttctca ccagaaagga catctgtgct ggtttcaatc
     1201 gcccctgcga gaccctgggc ctgtctcacc tttcaggaat gtgtcagcct caccgcagtt
     1261 gtaacatcaa tgaagattcg ggactccctc tggctttcac aattgcccat gagctaggac
     1321 acagcttcgg catccagcat gatgggaaag aaaatgactg tgagcctgtg ggcagacatc
     1381 cgtacatcat gtcccgccag ctccagtacg atcccactcc gctgacatgg tccaagtgca
     1441 gcgaggagta catcacccgc ttcttggacc gaggctgggg gttctgtctt gatgacatac
     1501 ctaaaaagaa aggcttgaag tccaaggtca ttgcccccgg agtgatctat gatgttcacc
     1561 accagtgcca gctacaatat ggacccaatg ctaccttctg ccaggaagta gaaaacgtct
     1621 gccagacact gtggtgctcc gtgaagggct tttgtcgctc taagctggac gctgctgcag
     1681 atggaactca atgtggtgag aagaagtggt gtatggcagg caagtgcatc acagtgggga
     1741 agaaaccaga gagcattcct ggaggctggg gccgctggtc accctggtcc cactgttcca
     1801 ggacctgtgg ggctggagtc cagagcgcag agaggctctg caacaacccc gagccaaagt
     1861 ttggagggaa atattgcact ggagaaagaa aacgctatcg cttgtgcaac gtccacccct
     1921 gtcgctcaga ggcaccaaca tttcggcaga tgcagtgcag tgaatttgac actgttccct
     1981 acaagaatga actctaccac tggtttccca tttttaaccc agcacatcct tgtgagctct
     2041 actgccgacc catagatggc cagttttctg agaaaatgct ggatgctgtc attgatggta
     2101 ccccttgctt tgaaggcggc aacagcagaa atgtctgtat taatggcata tgtaagatgg
     2161 ttggctgtga ctatgagatc gattccaatg ccaccgagga tcgctgcggt gtgtgcctgg
     2221 gagatggctc ttcctgccag actgtgagaa agatgtttaa gcagaaggaa ggatctggtt
     2281 atgttgacat tgggctcatt ccaaaaggag caagggacat aagagtgatg gaaattgagg
     2341 gagctggaaa cttcctggcc atcaggagtg aagatcctga aaaatattac ctgaatggag
     2401 ggtttattat ccagtggaac gggaactata agctggcagg gactgtcttt cagtatgaca
     2461 ggaaaggaga cctggaaaag ctgatggcca caggtcccac caatgagtct gtgtggatcc
     2521 agcttctatt ccaggtgact aaccctggca tcaagtatga gtacacaatc cagaaagatg
     2581 gccttgacaa tgatgttgag cagcagatgt acttctggca gtacggccac tggacagagt
     2641 gcagtgtgac ctgcgggaca ggtatccgcc gccaaactgc ccattgcata aagaagggcc
     2701 gcgggatggt gaaagctaca ttctgtgacc cagaaacaca gcccaatggg agacagaaga
     2761 agtgccatga aaaggcttgt ccacccaggt ggtgggcagg ggagtgggaa gcatgctcgg
     2821 cgacatgcgg gccccacggg gagaagaagc gaaccgtgct gtgcatccag accatggtct
     2881 ctgacgagca ggctctcccg cccacagact gccagcacct gctgaagccc aagaccctcc
     2941 tttcctgcaa cagagacatc ctgtgcccct cggactggac agtgggcaac tggagtgagt
     3001 gttctgtttc ctgtggtggt ggagtgcgga ttcgcagtgt cacatgtgcc aagaaccatg
     3061 atgaaccttg cgatgtgaca aggaaaccca acagccgagc tctgtgtggc ctccagcaat
     3121 gcccttctag ccggagagtt ctgaaaccaa acaaaggcac tatttccaat ggaaaaaacc
     3181 caccaacact aaagcccgtc cctccaccta catccaggcc cagaatgctg accacaccca
     3241 cagggcctga gtctatgagc acaagcactc cagcaatcag cagccctagt cctaccacag
     3301 cctccaaaga aggagacctg ggtgggaaac agtggcaaga tagctcaacc caacctgagc
     3361 tgagctctcg ctatctcatt tccactggaa gcacttccca gcccatcctc acttcccaat
     3421 ccttgagcat tcagccaagt gaggaaaatg tttccagttc agatactggt cctacctcgg
     3481 agggaggcct tgtagctaca acaacaagtg gttctggctt gtcatcttcc cgcaacccta
     3541 tcacttggcc tgtgactcca ttttacaata ccttgaccaa aggtccagaa atggagattc
     3601 acagtggctc aggggaagaa agagaacagc ctgaggacaa agatgaaagc aatcctgtaa
     3661 tatggaccaa gatcagagta cctggaaatg acgctccagt ggaaagtaca gaaatgccac
     3721 ttgcacctcc actaacacca gatctcagca gggagtcctg gtggccaccc ttcagcacag
     3781 taatggaagg actgctcccc agccaaaggc ccactacttc cgaaactggg acacccagag
     3841 ttgaggggat ggttactgaa aagccagcca acactctgct ccctctggga ggagaccacc
     3901 agccagaacc ctcaggaaag acggcaaacc gtaaccacct gaaacttcca aacaacatga
     3961 accaaacaaa aagttctgaa ccagtcctga ctgaggagga tgcaacaagt ctgattactg
     4021 agggcttttt gctaaatgcc tccaattaca agcagctcac aaacggccac ggctctgcac
     4081 actggatcgt cggaaactgg agcgagtgct ccaccacatg tggcctgggg gcctactgga
     4141 gaagggtgga gtgcagcacc cagatggatt ctgactgtgc ggccatccag agacctgacc
     4201 ctgcaaaaag atgccacctc cgtccctgtg ctggctggaa agtgggaaac tggagcaagt
     4261 gctccagaaa ctgcagtggg ggcttcaaga tacgcgagat tcagtgcgtg gacagccggg
     4321 accaccggaa cctgaggcca tttcactgcc agttcctggc cggcattcct cccccattga
     4381 gcatgagctg taacccggag ccctgtgagg cgtggcaggt ggagccttgg agccagtgct
     4441 ccaggtcctg tggaggtgga gttcaggaga gaggagtgtt ctgtccagga ggcctctgtg
     4501 attggacaaa aagacccaca tccaccatgt cttgcaatga gcacctgtgc tgtcactggg
     4561 ccactgggaa ctgggacctg tgttccactt cctgtggagg tggctttcag aagaggactg
     4621 tccaatgtgt gccctcagag ggcaataaaa ctgaagacca agaccaatgt ctatgtgatc
     4681 acaaacccag acctccagaa ttcaaaaaat gcaaccagca ggcctgcaag aaaagtgccg
     4741 atttactttg cactaaggac aaactgtcag ccagtttctg ccagacactg aaagccatga
     4801 agaaatgttc tgtgcccacc gtgagggctg agtgctgctt ctcgtgtccc cagacacaca
     4861 tcacacacac ccaaaggcaa agaaggcaac ggttgctcca aaagtcaaaa gaactctaag
     4921 cccaaaagga agccagcttc caccagactg cagcagcctg ctgaccatga catgctttag
     4981 ccctgggagc tgcttcgttg aggttttgct tcatatattt cagattcagc tttgggtcat
     5041 gatgaaacaa aatccattgt gttcctctag ccaccgaatg tccctcacag gaggcctagg
     5101 ctgccgtact tgctgctccc tgataggaag gtgaaatagc taggagaatc aggcagcacc
     5161 tggtatcggc tcctgggaaa ggtgccaggt tagacagatt tgtgaaatac tgtatttttg
     5221 ttctccctta aagataaaaa caaagagacc agctatttcc ctgactacct caagagtatc
     5281 aggcaacact tacccagggg acaaataatc ttcttaattt atcttgatga atacctttct
     5341 tttttttttc ttgcctccct gcctggggca gctgctattt aaaatgtcac agcggagctt
     5401 taggaacatt cctttaattc atccaggcag aggactgctt tcttcacccc atgttcaact
     5461 agggcaggaa gtgctggtta catccctcag tctcttcctc catccctgta gggctccata
     5521 tctgggctgc ttagagtctc ctgccaacct tgggacacta gactaagagg aaacaggcat
     5581 aagaaacaca gcctaagggg tttaggtcac ttacacagaa aacacttctg gtttgaagag
     5641 catttctatg aaaactcgta aaatgcaaat gagcagaaat caatttctta atattacaga
     5701 aaaggaactg tgggccttta ttcttcccac tttcctggca aatacacact cctttgcaga
     5761 agggaaaatg tgtcttcctg gctgacttcc tcccatcctc cagtccccac aggaggcctg
     5821 agctcaggtc ccaccatcgt cttgctgcag gggctgtata tgcaaagacc actatgacca
     5881 gctctagggc tgccacctgt cctcacctct tctcaggttg gaaaatggag gttccatatt
     5941 aaaatatttc aaagaaccag gtaaattgta ctaagtacaa gaacatagtg caaacttcta
     6001 atctagagaa aaaacatttc taaaaagtaa ggtaaagggg atctactcct cagtgtaccc
     6061 aatatgtagt tagtcttaaa gatcttttac tttggggaga acaggtgaat cagggagtcc
     6121 aaagaaactg aaatgaaagt tagaatgctt gttctggatg ttcctacctc ccaccagaag
     6181 tcaaagtagg cttggtctca aattcagagc aaatttctag acaaagtgat ttctaaggga
     6241 aggtacttac atgaactgaa cccagagaaa gagggtaagt gggtgggtgg aggggcataa
     6301 gcttcaaatg aaagaagata tcagaaccaa ttcttgacag ggagaaaggc aaggatgtgg
     6361 ccttcatgtg atgggcagca tattttctga ctcatgcaca gatacactta atactcctca
     6421 tggaacacaa tatcccacgt ctccctttac ctatccgttc agcagaatgg tgctattaaa
     6481 aaaatggtgc taatatctag atggtggcag aattaatgta aacatagata ggcatacaaa
     6541 tgggtaagtg tatgtgaaac tctccatata catcttctgt taaacatgta acggtgttgc
     6601 tgaccaatgc cagttttatt gcttgggtac cttgaattta agatttaata ttatgtgctt
     6661 tttatttttt tccaagccat taacgtgtat tactctcatg aattcctgtg aaaaataaaa
     6721 cagtgggatt gggatgaaat tcaagataga ccagaaacag gaggaaagta caaatgttta
     6781 cctaaagcca gagaatttat ttctagatga taattacatt attgtattct ctgcctttaa
     6841 acataaacat tgctggccga tagcaatctc tggtgaataa tggttttcga ggagaggaga
     6901 cacttaaatt gttagtgaca tgagtggagg agaatctgat atttactaat gaggcagttt
     6961 gcaaagactg tccctgaagt gtagtgtagt ctttcagggg gattcattta gtaagctaga
     7021 ttgatttaac ctggtactgt actagcatag ggtcaaatac gtgtcatcag agacctgggt
     7081 atgaccaggc ttacaaaact caggaacaaa ctcagattcc tactgacctc aaccaactaa
     7141 actaggccaa atttctccgt gtacaaaatg gaccacgtta tttacaatcc acgtggtgga
     7201 gaagatggta gacatgtgga gagagtttgg ccaggtgctc cattctaggt ctttttccag
     7261 tttctcaaag gcagaacatt ggctcctaat tatttgccat ggatatttgc ttcctgttct
     7321 ggagctatgt tgtaagacag ctgtgtgatg tccatcattc ttgactccag aatgaagaca
     7381 gggcttgctg ttttctgtcc tgttggtatc atctgttgcc ttggcgatca ctcagtgatg
     7441 agtcgtcagt attttgacat gtcccagtgc ttgctgtaca agaggggact cagatcaaca
     7501 ggaagactct gaagacagga acctgcatgg tatcttacat ctttgatact tgggtgctga
     7561 tatgaagcag agttgttgat ttactttatc taggcccttc tttcttctca cctggatcaa
     7621 gcaactgaga agtgtatcag gagacactgg atacatatct ctatgaaata gagagggcca
     7681 tcgcagggcc tggtacttaa ctacattaac gttctaaaac ccagtttggt ttacgttgtc
     7741 tttcacagta gtatatttag ctcttctctg gaaagttgtg ggttaatata attcttaaac
     7801 atgaaaatgt aattaaacac accacgagag aacaatattc caggagactt aatagtgatt
     7861 actttcttca atcaggaaat cgtttcagtg cctcctttgt aggaatgctt tgttttgtga
     7921 tgggttttct taaagaagag cacacctccg tccaatctcc tgagacagcc acgtctccgc
     7981 tgacatccca ctgtgatgct ttcagatagt cagtgaatgt ttctgataac cttcatccag
     8041 tatctgaaac acaatgtgag agattatatt gttttagata ataacatccc atttagttga
     8101 ctaaaatctt ccaaactctg aaagctgcac actgctactc cagagagtgc aggtcttagc
     8161 tcttctcctt tctgacttca agatgaatct ttgggacgat gtttctggtg cttggtccac
     8221 agtgattcac ttttgaagga gaggccacat gacatgaact gcctggtgtt acaacctagc
     8281 taacatattt gatgctactc ctgttgtctg tactgcttat tcaagtagta ttctaagtta
     8341 tgttactaaa aaacatggtg ggtaaagcac aatcctaccc atcattgtcc tccaaaataa
     8401 ttgtatgaca tacacggccc agcccattgc cctccctgca tctctgtgct gctttgccat
     8461 ttccccttct acccagcctc ctcaaggggt accttggtgg atatttcagt acttaaaacc
     8521 agactgtaat cataacctcc ctctgtgtgg catcaataaa tagccaaact ca
//
