LOCUS       NM_014265               7019 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens ADAM metallopeptidase domain 28 (ADAM28), transcript
            variant 1, mRNA.
ACCESSION   NM_014265 NM_021778
VERSION     NM_014265.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Xie,Y., Zheng,Z.W., He,H.T. and Chang,Z.B.
  TITLE     LncRNA NEAT1 induces autophagy through the miR-128-3p/ADAM28 axis
            to suppress apoptosis of nonsmall-cell lung cancer
  JOURNAL   Kaohsiung J Med Sci 38 (10), 933-949 (2022)
   PUBMED   36054559
  REMARK    GeneRIF: LncRNA NEAT1 induces autophagy through the
            miR-128-3p/ADAM28 axis to suppress apoptosis of nonsmall-cell lung
            cancer.
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Yin,Q., Gu,J., Qi,Y., Lu,Y., Yang,L., Liu,J. and Liang,X.
  TITLE     ADAM28 from both endothelium and gastric cancer cleaves von
            Willebrand Factor to eliminate von Willebrand Factor-induced
            apoptosis of gastric cancer cells
  JOURNAL   Eur J Pharmacol 898, 173994 (2021)
   PUBMED   33675784
  REMARK    GeneRIF: ADAM28 from both endothelium and gastric cancer cleaves
            von Willebrand Factor to eliminate von Willebrand Factor-induced
            apoptosis of gastric cancer cells.
REFERENCE   3  (bases 1 to 7019)
  AUTHORS   Hubeau,C., Rocks,N. and Cataldo,D.
  TITLE     ADAM28: Another ambivalent protease in cancer
  JOURNAL   Cancer Lett 494, 18-26 (2020)
   PUBMED   32861707
  REMARK    GeneRIF: ADAM28: Another ambivalent protease in cancer.
            Review article
REFERENCE   4  (bases 1 to 7019)
  AUTHORS   Zhao,Z., Qiu,H.Y., Fu,L. and Li,J.
  TITLE     [Construction of ADAM28 shRNA interference vector and its
            inhibitory effect on human periodontal ligament stem cells]
  JOURNAL   Shanghai Kou Qiang Yi Xue 29 (5), 476-481 (2020)
   PUBMED   33543212
  REMARK    GeneRIF: [Construction of ADAM28 shRNA interference vector and its
            inhibitory effect on human periodontal ligament stem cells].
REFERENCE   5  (bases 1 to 7019)
  AUTHORS   Fourie,A.M., Coles,F., Moreno,V. and Karlsson,L.
  TITLE     Catalytic activity of ADAM8, ADAM15, and MDC-L (ADAM28) on
            synthetic peptide substrates and in ectodomain cleavage of CD23
  JOURNAL   J Biol Chem 278 (33), 30469-30477 (2003)
   PUBMED   12777399
  REMARK    GeneRIF: ADAM8, ADAM15, and ADAM28, but not ADAM17, catalyzed
            ectodomain shedding of CD23, the low affinity IgE receptor.
REFERENCE   6  (bases 1 to 7019)
  AUTHORS   Bridges,L.C., Hanson,K.R., Tani,P.H., Mather,T. and Bowditch,R.D.
  TITLE     Integrin alpha4beta1-dependent adhesion to ADAM 28 (MDC-L) requires
            an extended surface of the disintegrin domain
  JOURNAL   Biochemistry 42 (13), 3734-3741 (2003)
   PUBMED   12667064
REFERENCE   7  (bases 1 to 7019)
  AUTHORS   Bridges,L.C., Tani,P.H., Hanson,K.R., Roberts,C.M., Judkins,M.B.
            and Bowditch,R.D.
  TITLE     The lymphocyte metalloprotease MDC-L (ADAM 28) is a ligand for the
            integrin alpha4beta1
  JOURNAL   J Biol Chem 277 (5), 3784-3792 (2002)
   PUBMED   11724793
REFERENCE   8  (bases 1 to 7019)
  AUTHORS   Howard,L., Maciewicz,R.A. and Blobel,C.P.
  TITLE     Cloning and characterization of ADAM28: evidence for autocatalytic
            pro-domain removal and for cell surface localization of mature
            ADAM28
  JOURNAL   Biochem J 348 Pt 1 (Pt 1), 21-27 (2000)
   PUBMED   10794709
REFERENCE   9  (bases 1 to 7019)
  AUTHORS   Jury,J.A., Perry,A.C. and Hall,L.
  TITLE     Identification, sequence analysis and expression of transcripts
            encoding a putative metalloproteinase, eMDC II, in human and
            macaque epididymis
  JOURNAL   Mol Hum Reprod 5 (12), 1127-1134 (1999)
   PUBMED   10587367
REFERENCE   10 (bases 1 to 7019)
  AUTHORS   Roberts,C.M., Tani,P.H., Bridges,L.C., Laszik,Z. and Bowditch,R.D.
  TITLE     MDC-L, a novel metalloprotease disintegrin cysteine-rich protein
            family member expressed by human lymphocytes
  JOURNAL   J Biol Chem 274 (41), 29251-29259 (1999)
   PUBMED   10506182
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA933009.1, AJ242015.1,
            AC120193.5, BE833805.1 and BM992381.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_014265.5.
            
            Summary: This gene encodes a member of the ADAM (a disintegrin and
            metalloprotease domain) family. Members of this family are
            membrane-anchored proteins structurally related to snake venom
            disintegrins, and have been implicated in a variety of biological
            processes involving cell-cell and cell-matrix interactions,
            including fertilization, muscle development, and neurogenesis. The
            protein encoded by this gene is a lymphocyte-expressed ADAM
            protein. This gene is present in a gene cluster with other members
            of the ADAM family on chromosome 8. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1, also known as MDC-Lm) encodes
            the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.136616.1,
                                           SRR1803616.56624.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265769.9/ ENSP00000265769.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DA933009.1         30-67
            39-2373             AJ242015.1         5-2339
            2374-2374           AC120193.5         56727-56727
            2375-2491           AJ242015.1         2341-2457
            2492-2495           BE833805.1         398-401
            2496-3086           AJ242015.1         2458-3048
            3087-6823           AC120193.5         57991-61727
            6824-7019           BM992381.1         23-218              c
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.2"
     gene            1..7019
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="ADAM metallopeptidase domain 28"
                     /db_xref="GeneID:10863"
                     /db_xref="HGNC:HGNC:206"
                     /db_xref="MIM:606188"
     exon            1..127
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..2409
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; disintegrin and metalloproteinase
                     domain-containing protein 28; metalloproteinase-like,
                     disintegrin-like, and cysteine-rich protein-L; epididymial
                     metalloproteinase-like, disintegrin-like, and
                     cysteine-rich protein II; epididymal
                     metalloproteinase-like, disintegrin-like, and
                     cysteine-rich protein II; epididymis secretory sperm
                     binding protein"
                     /codon_start=1
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 28 isoform 1 preproprotein"
                     /protein_id="NP_055080.2"
                     /db_xref="CCDS:CCDS34865.1"
                     /db_xref="GeneID:10863"
                     /db_xref="HGNC:HGNC:206"
                     /db_xref="MIM:606188"
                     /translation="MLQGLLPVSLLLSVAVSAIKELPGVKKYEVVYPIRLHPLHKREA
                     KEPEQQEQFETELKYKMTINGKIAVLYLKKNKNLLAPGYTETYYNSTGKEITTSPQIM
                     DDCYYQGHILNEKVSDASISTCRGLRGYFSQGDQRYFIEPLSPIHRDGQEHALFKYNP
                     DEKNYDSTCGMDGVLWAHDLQQNIALPATKLVKLKDRKVQEHEKYIEYYLVLDNGEFK
                     RYNENQDEIRKRVFEMANYVNMLYKKLNTHVALVGMEIWTDKDKIKITPNASFTLENF
                     SKWRGSVLSRRKRHDIAQLITATELAGTTVGLAFMSTMCSPYSVGVVQDHSDNLLRVA
                     GTMAHEMGHNFGMFHDDYSCKCPSTICVMDKALSFYIPTDFSSCSRLSYDKFFEDKLS
                     NCLFNAPLPTDIISTPICGNQLVEMGEDCDCGTSEECTNICCDAKTCKIKATFQCALG
                     ECCEKCQFKKAGMVCRPAKDECDLPEMCNGKSGNCPDDRFQVNGFPCHHGKGHCLMGT
                     CPTLQEQCTELWGPGTEVADKSCYNRNEGGSKYGYCRRVDDTLIPCKANDTMCGKLFC
                     QGGSDNLPWKGRIVTFLTCKTFDPEDTSQEIGMVANGTKCGDNKVCINAECVDIEKAY
                     KSTNCSSKCKGHAVCDHELQCQCEEGWIPPDCDDSSVVFHFSIVVGVLFPMAVIFVVV
                     AMVIRHQSSREKQKKDQRPLSTTGTRPHKQKRKPQMVKAVQPQEMSQMKPHVYDLPVE
                     GNEPPASFHKDTNALPPTVFKDNPVSTPKDSNPKA"
     exon            128..231
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            232..308
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            309..387
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            388..464
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            465..657
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            658..729
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            730..801
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            802..971
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1053
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1184
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1362
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1452
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1648
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1751
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1911
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..1992
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2071
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2180
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2259
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2260..2325
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2388
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..7019
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2823..2828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: ATTACA"
     polyA_site      2847
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="major polyA site"
     regulatory      6127..6132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: ATTAAA"
     polyA_site      6153
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="major polyA site"
     regulatory      6168..6173
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: AGTAAA"
     polyA_site      6188
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
     regulatory      6817..6822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: ATTAAA"
     polyA_site      6843
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
     regulatory      6845..6850
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: ATTAAA"
     polyA_site      6866
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
     regulatory      6885..6890
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /note="hexamer: ATTAAA"
     polyA_site      6902
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
ORIGIN      
        1 actggagagg aggcagggac agacccagca gcacccacct gagcgagaag agcagacacc
       61 gtgctcctgg aatcacccag catgttgcaa ggtctcctgc cagtcagtct cctcctctct
      121 gttgcagtaa gtgctataaa agaactccct ggggtgaaga agtatgaagt ggtttatcct
      181 ataagacttc atccactgca taaaagagag gccaaagagc cagagcaaca ggaacaattt
      241 gaaactgaat taaagtataa aatgacaatt aatggaaaaa ttgcagtgct ttatttgaaa
      301 aaaaacaaga acctccttgc accaggctac acggaaacat attataattc cactggaaag
      361 gagatcacca caagcccaca aattatggat gattgttatt atcaaggaca tattcttaat
      421 gaaaaggttt ctgacgctag catcagcaca tgtaggggtc taaggggcta cttcagtcag
      481 ggggatcaaa gatactttat tgaaccttta agccccatac atcgggatgg acaggagcat
      541 gcactcttca agtataaccc tgatgaaaag aattatgaca gcacctgtgg gatggatggt
      601 gtgttgtggg cccacgattt gcagcagaac attgccctac ctgccaccaa actagtaaaa
      661 ttgaaagaca ggaaggttca ggaacatgag aaatacatag aatattattt ggtcctggat
      721 aatggtgagt ttaaaaggta caatgagaat caagatgaga tcagaaagag ggtatttgag
      781 atggctaatt atgtcaacat gctttataaa aagctcaata ctcatgtggc cttagttggt
      841 atggaaatct ggactgacaa ggataagata aagataaccc caaatgcaag cttcaccttg
      901 gagaattttt ctaaatggag ggggagtgtt ctctcaagaa gaaagcgtca tgatattgct
      961 cagttaatca cagcaacaga acttgctgga acgactgtgg gtcttgcatt tatgtctaca
     1021 atgtgttctc cttattctgt tggcgttgtt caggaccaca gcgataatct tcttagagtt
     1081 gcagggacaa tggcacatga aatgggccac aactttggaa tgtttcatga cgactattct
     1141 tgcaagtgtc cttctacaat atgtgtgatg gacaaagcac tgagcttcta tatacccaca
     1201 gacttcagtt cctgcagccg tctcagctat gacaagtttt ttgaagataa attatcaaat
     1261 tgcctcttta atgctccatt gcctacagat atcatatcca ctccaatttg tgggaaccag
     1321 ttggtggaaa tgggagagga ctgtgattgt gggacatctg aggaatgtac caatatttgc
     1381 tgtgatgcta agacatgtaa aatcaaagca acttttcaat gtgcattagg agaatgttgt
     1441 gaaaaatgcc aatttaaaaa ggctgggatg gtgtgcagac cagcaaaaga tgagtgcgac
     1501 ctgcctgaaa tgtgtaatgg taaatctggt aattgtcctg atgatagatt ccaagtcaat
     1561 ggcttccctt gccatcacgg gaagggccac tgcttgatgg ggacatgccc cacactgcag
     1621 gagcagtgca cagagctgtg gggaccagga actgaggttg cagataagtc atgttacaac
     1681 aggaatgaag gtgggtcaaa gtacgggtac tgtcgcagag tggatgacac actcattccc
     1741 tgcaaagcaa atgataccat gtgtgggaag ttgttctgtc aaggtgggtc ggataatttg
     1801 ccctggaaag gacggatagt gactttcctg acatgtaaaa catttgatcc tgaagacaca
     1861 agtcaagaaa taggcatggt ggccaatgga actaagtgtg gcgataacaa ggtttgcatt
     1921 aatgcagaat gtgtggatat tgagaaagcc tacaaatcaa ccaattgctc atctaagtgc
     1981 aaaggacatg ctgtgtgtga ccatgagctc cagtgtcaat gtgaggaagg atggatccct
     2041 cccgactgcg atgactcctc agtggtcttc cacttctcca ttgtggttgg ggtgctgttc
     2101 ccaatggcgg tcatttttgt ggtggttgct atggtaatcc ggcaccagag ctccagagaa
     2161 aagcagaaga aagatcagag gccactatct accactggca ccaggccaca caaacagaag
     2221 aggaaacccc agatggtaaa ggctgttcaa ccccaagaga tgagtcagat gaagccccat
     2281 gtgtatgatc tgccagtaga aggcaatgag cccccagcct cttttcataa agacacaaac
     2341 gcacttcccc ctactgtttt caaggataat ccagtgtcta cacctaagga ctcaaatcca
     2401 aaagcatgaa gcaacagcta agcaagaact aatggctaaa ttatcaactt ggaaaactgg
     2461 aaaatctgga tggcagagaa atatactatc tatctcacca gtatttgctc tcgactcaag
     2521 aaggttaaca ttttctgatt catgttagac tttgaagaga ctaaagaaaa ttttcaagag
     2581 gaacatatgc ctgagaacct ttgcatgaat ttaaaatttc aattatccat tcttataaga
     2641 aggaagatga ttgtaaagaa atatctccga agttaaaatc tgtaatagga attgattcat
     2701 tctctaatga aaacaaaaca taaaaacatc acactaatct tggaggaata agaaaaattg
     2761 tacatccatt aaatgtacaa ttgattgcaa catcttgatt gttttaacca ttaacttgtc
     2821 aaattacaat cacagttaag aaaatgatgt aaaattctgt tttgtggatc tctttcctag
     2881 attagcttct gaaatcatta ttagctatat catttgaggt tttctacaat ttggtataac
     2941 taagaattta aaaatgtttt atcatatata tttgtataat taattactgg catggttaaa
     3001 gtggttttca ctttttaaat ggagaaaatt tcagttaaat taataggata aaccaggttg
     3061 cgaactggtg acctgtaggc catgtttgca ctgcaaatat atttggtctg aatgatattg
     3121 atattggaca catagtactt ttacatgttt tgaatgtatt gctaatattt aaaaattgag
     3181 agatcttgca taaacaatag attcccagct ttgtcagatg taatctaaaa gtaatccgat
     3241 gctttgtcaa caacagactt acattgccca tagcaaccgt cagcttagtt gatgggtaat
     3301 gtagcaaaca ctattggttt cctaccaaat aatccccttt ttcctggctt ttagaacaat
     3361 aattttgttc acctgcccac ttctaggaaa gaatatccta tctaatctat ctatctcagg
     3421 gataaatccc tatcctttta agtaagtcaa gtcaattaaa ataacctcat ttttccttgc
     3481 tagtggtcga cttggagatg attttataat cctccccctc cctttttttt tttgctagta
     3541 agacgacaga ggaattatgt tacaacactt ctggaaaatt tttcctcgtc tctttttctg
     3601 gatatgggca tctgaatact ctagctggta ctgctgcgct ccacacggtg attatgaagg
     3661 ctgaaaaaga tagatagagc agaaagatga aaagaataaa acctggcctt tcaatgatgt
     3721 catggaattg gtagatcagt gactgctgga gttacctcac actttcttgg tactccttat
     3781 tatatgagcc atacattttc cttgttgttt aagccatttt gaattggatt ttctctcact
     3841 ttcaacagag atcatcctaa ctagtaaagt agctgtttcc ataatcaggg aacatgcttt
     3901 agttcatcat cacagctaca gtttttcttg tatttgtctg cttcacccat ttctatcaat
     3961 cacttacctg gtccccacag gcctgtcgct tcatgaggaa ctttatggtt catttaccct
     4021 tgcagtatga ggttctagat aagcccactc tggcctcaaa agtgaagctc agtcctcagg
     4081 aaatccagag tccactgatc aaatcactgt caaggataca ggctttttct ccatgtggca
     4141 tttgcagaag aacaacttta aaagtagagc tttatctgcc ctgtgaggtt tcagaagacc
     4201 ctcccctgac ccagaaataa atccctattt cctggaaagc cattatgtac tcgtgaccaa
     4261 cttgtgaact tttctgtgtt gttgttgttg ttttttaaat atctgtcccc agtaatagat
     4321 tcacctaatc cttttttata ctgatggaat ggaaatctta aatctatgaa tcctcaacta
     4381 gccatgtacc ataacccaag caaaaactaa accatacact ctcaaaagca gtggcatgtg
     4441 aacatgcctg caagttccta aatgatgtca ctaggtccct gatgcagatg catcatacgt
     4501 cctatttgaa gagaagaaat gcagtacaat gacaggaaaa ccattatttt atcatagaac
     4561 tgcgaaccca agtcctacaa tgtttccctt tcagctcaac tgcagaatag tttagaacag
     4621 actttttgat ttcacaatac tgtttccaca cttacttata tccgtaactt tctaacttta
     4681 agggaattcc gcttatttga catttaacta cctcagatat gccaccaaac ctgttttcat
     4741 cattagttat ttttaccttg tctactttaa aacagtgaaa ttattaggtt aaattatgtt
     4801 tttatccagt tactctaaga taccttagtg tttgtctact tattacaact caccaaatta
     4861 ctggttttat atggcctttg ggaaggcttt tagcttttta taaaataatg aagtatgcat
     4921 gaaaagaaag caaattttag ttggttgcag tcagagaata tggaaagggt tattattata
     4981 attaggattt tattattcac attggaaatc actgctatgt gttaaggaat tgaaggtggc
     5041 ctctggctag cagccattaa gaaaatgagc ccctgagttc aacgaccctc aaatgaagtg
     5101 aagtctacca ataaccactg aataagcttg gaattttaga ttccttccca gctgaatctt
     5161 gacttcagcc ttgtgagaca ccctgaagta gaggatgcag ctaaattttg tccagattac
     5221 acacccacag aaactgtatg ataattaata tgtgttgttt aaagttgtta tggtttggag
     5281 atatttgtta tgcagcaata gatagctgat acaaggaagt agagaacaaa cattttctat
     5341 tagtctgttt tcacatggct ataaagaata ccacctgaga ctgggtaatt tataaagaaa
     5401 ggaggattaa ttgactcaga tttccacatg gcctaggagg cctcaggaaa cttacaatca
     5461 tgatgaaaga ggaagcagaa acatctcata tgatggaaga tgtgtatgaa ggaggaactg
     5521 tcaaacactt ataaaaccat cagatctctt gagagcttac tatctcgaca aaagcatggg
     5581 ggaaaccacc cccatgatcc aatcacctcc cacctggtcc ctccctcaac atgtggagat
     5641 tatagcaatt aaaaaaatga gatttggggg gatggacaca gagccaaacc atataatgtt
     5701 ttaaatgctc cagataggtt taggtaaaat taactatatt taataggctt tcatcactgc
     5761 aaaacttttt ttcctttctt tgtgtctcta gtctcttcca tttgttctcc cttctttact
     5821 agcaccgagg atttgcttga aatctctgat agccagtatt ctcatttatc atattctttc
     5881 tcactttgtg tttccttggt aattttggat aactccttaa atttatcttc caaattactg
     5941 gtttttatat tctgtactgt cagtcctcct gttcactaat ttcaaaattt atagttgttt
     6001 ctcttgttta cttatatagt tgtttctctt gtttacttaa taacattctc ttctttccta
     6061 tcagtttaat tttataaagg ctaaccaatt aactgttttt aacttatatt tccagatatt
     6121 ttaaaaatta aagcaaaatg tgtataaact ttattactgc ttcccacagt aaatattttt
     6181 cagaagtaca tgctttctca gccacttgac tagtatgttc cccatcattc aaagacacca
     6241 ttatttccta agtgttatat tgctattttt cagttggtgc atccaatatt attatcagct
     6301 ttaatattgt ttcatcatat gtcattgttc aaagaatcca tctcttgagc gtaagctaaa
     6361 taatttgcat acactagcat ctggatgtaa tatggaaaaa tatggaaatg gttggggtga
     6421 taagcaagtc ttgcatattc ttttctcctc aatttttttt ctccaaagtg ggccaagcca
     6481 agagtggcac agctgtgtgg agatactgag ctccaccttg tcagggaaga tcccaactca
     6541 ggagaaaaca cctctaacaa aatctgtagc cacgttggca catctgccct tgaattcact
     6601 ttgtctcttt tgctgcttcc ataaaaaggc tttcccttga caatgttagg cactttttag
     6661 caactaggcc tgggtggtct gctacaatgc ccaacactta atacagtatc actattactg
     6721 ttagtagtat gctaagcgaa tactattgtt ataaaaaaaa tcctaagcat gaaaattacg
     6781 gtatacaatt tattgtgaag gcaacttctt gtgaacatta aacggtttat ctccttcacc
     6841 tcatattaaa gtccatactc ttgggatata tctgacatct caatattaaa tgactaagat
     6901 gactaagtcg tttatgttgc tcccacctca cacaaagtta aaactatttg ttactttaaa
     6961 tagtttgaca atgttatttt aaaatctgga caaatcacaa atataaaata cttatttaa
//
