LOCUS       NP_055080                775 aa            linear   PRI 27-APR-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein 28
            isoform 1 preproprotein [Homo sapiens].
ACCESSION   NP_055080 NP_068548
VERSION     NP_055080.2
DBSOURCE    REFSEQ: accession NM_014265.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 775)
  AUTHORS   Xie,Y., Zheng,Z.W., He,H.T. and Chang,Z.B.
  TITLE     LncRNA NEAT1 induces autophagy through the miR-128-3p/ADAM28 axis
            to suppress apoptosis of nonsmall-cell lung cancer
  JOURNAL   Kaohsiung J Med Sci 38 (10), 933-949 (2022)
   PUBMED   36054559
  REMARK    GeneRIF: LncRNA NEAT1 induces autophagy through the
            miR-128-3p/ADAM28 axis to suppress apoptosis of nonsmall-cell lung
            cancer.
REFERENCE   2  (residues 1 to 775)
  AUTHORS   Yin,Q., Gu,J., Qi,Y., Lu,Y., Yang,L., Liu,J. and Liang,X.
  TITLE     ADAM28 from both endothelium and gastric cancer cleaves von
            Willebrand Factor to eliminate von Willebrand Factor-induced
            apoptosis of gastric cancer cells
  JOURNAL   Eur J Pharmacol 898, 173994 (2021)
   PUBMED   33675784
  REMARK    GeneRIF: ADAM28 from both endothelium and gastric cancer cleaves
            von Willebrand Factor to eliminate von Willebrand Factor-induced
            apoptosis of gastric cancer cells.
REFERENCE   3  (residues 1 to 775)
  AUTHORS   Hubeau,C., Rocks,N. and Cataldo,D.
  TITLE     ADAM28: Another ambivalent protease in cancer
  JOURNAL   Cancer Lett 494, 18-26 (2020)
   PUBMED   32861707
  REMARK    GeneRIF: ADAM28: Another ambivalent protease in cancer.
            Review article
REFERENCE   4  (residues 1 to 775)
  AUTHORS   Zhao,Z., Qiu,H.Y., Fu,L. and Li,J.
  TITLE     [Construction of ADAM28 shRNA interference vector and its
            inhibitory effect on human periodontal ligament stem cells]
  JOURNAL   Shanghai Kou Qiang Yi Xue 29 (5), 476-481 (2020)
   PUBMED   33543212
  REMARK    GeneRIF: [Construction of ADAM28 shRNA interference vector and its
            inhibitory effect on human periodontal ligament stem cells].
REFERENCE   5  (residues 1 to 775)
  AUTHORS   Fourie,A.M., Coles,F., Moreno,V. and Karlsson,L.
  TITLE     Catalytic activity of ADAM8, ADAM15, and MDC-L (ADAM28) on
            synthetic peptide substrates and in ectodomain cleavage of CD23
  JOURNAL   J Biol Chem 278 (33), 30469-30477 (2003)
   PUBMED   12777399
  REMARK    GeneRIF: ADAM8, ADAM15, and ADAM28, but not ADAM17, catalyzed
            ectodomain shedding of CD23, the low affinity IgE receptor.
REFERENCE   6  (residues 1 to 775)
  AUTHORS   Bridges,L.C., Hanson,K.R., Tani,P.H., Mather,T. and Bowditch,R.D.
  TITLE     Integrin alpha4beta1-dependent adhesion to ADAM 28 (MDC-L) requires
            an extended surface of the disintegrin domain
  JOURNAL   Biochemistry 42 (13), 3734-3741 (2003)
   PUBMED   12667064
REFERENCE   7  (residues 1 to 775)
  AUTHORS   Bridges,L.C., Tani,P.H., Hanson,K.R., Roberts,C.M., Judkins,M.B.
            and Bowditch,R.D.
  TITLE     The lymphocyte metalloprotease MDC-L (ADAM 28) is a ligand for the
            integrin alpha4beta1
  JOURNAL   J Biol Chem 277 (5), 3784-3792 (2002)
   PUBMED   11724793
REFERENCE   8  (residues 1 to 775)
  AUTHORS   Howard,L., Maciewicz,R.A. and Blobel,C.P.
  TITLE     Cloning and characterization of ADAM28: evidence for autocatalytic
            pro-domain removal and for cell surface localization of mature
            ADAM28
  JOURNAL   Biochem J 348 Pt 1 (Pt 1), 21-27 (2000)
   PUBMED   10794709
REFERENCE   9  (residues 1 to 775)
  AUTHORS   Jury,J.A., Perry,A.C. and Hall,L.
  TITLE     Identification, sequence analysis and expression of transcripts
            encoding a putative metalloproteinase, eMDC II, in human and
            macaque epididymis
  JOURNAL   Mol Hum Reprod 5 (12), 1127-1134 (1999)
   PUBMED   10587367
REFERENCE   10 (residues 1 to 775)
  AUTHORS   Roberts,C.M., Tani,P.H., Bridges,L.C., Laszik,Z. and Bowditch,R.D.
  TITLE     MDC-L, a novel metalloprotease disintegrin cysteine-rich protein
            family member expressed by human lymphocytes
  JOURNAL   J Biol Chem 274 (41), 29251-29259 (1999)
   PUBMED   10506182
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA933009.1, AJ242015.1,
            AC120193.5, BE833805.1 and BM992381.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 18, 2006 this sequence version replaced NP_055080.1.
            
            Summary: This gene encodes a member of the ADAM (a disintegrin and
            metalloprotease domain) family. Members of this family are
            membrane-anchored proteins structurally related to snake venom
            disintegrins, and have been implicated in a variety of biological
            processes involving cell-cell and cell-matrix interactions,
            including fertilization, muscle development, and neurogenesis. The
            protein encoded by this gene is a lymphocyte-expressed ADAM
            protein. This gene is present in a gene cluster with other members
            of the ADAM family on chromosome 8. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1, also known as MDC-Lm) encodes
            the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.136616.1,
                                           SRR1803616.56624.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265769.9/ ENSP00000265769.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.2"
     Protein         1..775
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 28 isoform 1 preproprotein"
                     /note="disintegrin and metalloproteinase domain-containing
                     protein 28; metalloproteinase-like, disintegrin-like, and
                     cysteine-rich protein-L; epididymial
                     metalloproteinase-like, disintegrin-like, and
                     cysteine-rich protein II; epididymal
                     metalloproteinase-like, disintegrin-like, and
                     cysteine-rich protein II; epididymis secretory sperm
                     binding protein"
                     /calculated_mol_wt=85355
     sig_peptide     1..18
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=1811
     Region          29..156
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          167..174
                     /region_name="Cysteine switch. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     mat_peptide     199..775
                     /product="Disintegrin and metalloproteinase
                     domain-containing protein 28. /id=PRO_0000029129"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UKQ2.3)"
                     /calculated_mol_wt=64514
     Region          204..397
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            268
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Site            275
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Site            order(339..340,343,349)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          416..488
                     /region_name="Disintegrin"
                     /note="pfam00200"
                     /db_xref="CDD:459709"
     Region          493..620
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     Site            557
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Site            602
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Site            628
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Site            666..686
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     Region          690..775
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9UKQ2.3)"
     CDS             1..775
                     /gene="ADAM28"
                     /gene_synonym="ADAM 28; eMDC II; eMDCII; MDC-L; MDCL"
                     /coded_by="NM_014265.6:82..2409"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS34865.1"
                     /db_xref="GeneID:10863"
                     /db_xref="HGNC:HGNC:206"
                     /db_xref="MIM:606188"
ORIGIN      
        1 mlqgllpvsl llsvavsaik elpgvkkyev vypirlhplh kreakepeqq eqfetelkyk
       61 mtingkiavl ylkknknlla pgytetyyns tgkeittspq imddcyyqgh ilnekvsdas
      121 istcrglrgy fsqgdqryfi eplspihrdg qehalfkynp deknydstcg mdgvlwahdl
      181 qqnialpatk lvklkdrkvq ehekyieyyl vldngefkry nenqdeirkr vfemanyvnm
      241 lykklnthva lvgmeiwtdk dkikitpnas ftlenfskwr gsvlsrrkrh diaqlitate
      301 lagttvglaf mstmcspysv gvvqdhsdnl lrvagtmahe mghnfgmfhd dysckcpsti
      361 cvmdkalsfy iptdfsscsr lsydkffedk lsnclfnapl ptdiistpic gnqlvemged
      421 cdcgtseect niccdaktck ikatfqcalg eccekcqfkk agmvcrpakd ecdlpemcng
      481 ksgncpddrf qvngfpchhg kghclmgtcp tlqeqctelw gpgtevadks cynrneggsk
      541 ygycrrvddt lipckandtm cgklfcqggs dnlpwkgriv tfltcktfdp edtsqeigmv
      601 angtkcgdnk vcinaecvdi ekaykstncs skckghavcd helqcqceeg wippdcddss
      661 vvfhfsivvg vlfpmavifv vvamvirhqs srekqkkdqr plsttgtrph kqkrkpqmvk
      721 avqpqemsqm kphvydlpve gneppasfhk dtnalpptvf kdnpvstpkd snpka
//
