LOCUS       NM_003813               2647 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ADAM metallopeptidase domain 21 (ADAM21), mRNA.
ACCESSION   NM_003813
VERSION     NM_003813.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Honda,H., Takamura,M., Yamagiwa,S., Genda,T., Horigome,R.,
            Kimura,N., Setsu,T., Tominaga,K., Kamimura,H., Matsuda,Y.,
            Wakai,T., Aoyagi,Y. and Terai,S.
  TITLE     Overexpression of a disintegrin and metalloproteinase 21 is
            associated with motility, metastasis, and poor prognosis in
            hepatocellular carcinoma
  JOURNAL   Sci Rep 7 (1), 15485 (2017)
   PUBMED   29138461
  REMARK    GeneRIF: ADAM21 was overexpressed in KYN-2 hepatocellular carcinoma
            (HCC) cell line, and its knockdown reduced invasion, migration,
            proliferation, and metastasis relative to controls. In HCC clinical
            specimens, ADAM21 positivity was associated with vascular invasion,
            large tumor size, high histological grade, and lower overall and
            recurrence-free survival as compared to cases that were negative
            for ADAM21 expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2647)
  AUTHORS   Poindexter,K., Nelson,N., DuBose,R.F., Black,R.A. and Cerretti,D.P.
  TITLE     The identification of seven metalloproteinase-disintegrin (ADAM)
            genes from genomic libraries
  JOURNAL   Gene 237 (1), 61-70 (1999)
   PUBMED   10524237
REFERENCE   4  (bases 1 to 2647)
  AUTHORS   Hooft van Huijsduijnen,R.
  TITLE     ADAM 20 and 21; two novel human testis-specific membrane
            metalloproteases with similarity to fertilin-alpha
  JOURNAL   Gene 206 (2), 273-282 (1998)
   PUBMED   9469942
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB035527.1, AL391223.4,
            AL357153.4 and AA758110.1.
            
            On May 17, 2019 this sequence version replaced NM_003813.3.
            
            Summary: This gene encodes a member of the ADAM (a disintegrin and
            metalloprotease domain) family. Members of this family are
            membrane-anchored proteins structurally related to snake venom
            disintegrins, and have been implicated in a variety of biological
            processes involving cell-cell and cell-matrix interactions,
            including fertilization, muscle development, and neurogenesis. The
            expression of this gene expression is testis-specific. [provided by
            RefSeq, May 2011].
            
            ##Evidence-Data-START##
            Transcript exon combination :: DB051707.1, DB035527.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148093
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000603540.2/ ENSP00000474385.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               DB035527.1         18-203
            187-1301            AL391223.4         16563-17677
            1302-2598           AL357153.4         2-1298
            2599-2647           AA758110.1         3-51                c
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.2"
     gene            1..2647
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /note="ADAM metallopeptidase domain 21"
                     /db_xref="GeneID:8747"
                     /db_xref="HGNC:HGNC:200"
                     /db_xref="MIM:603713"
     exon            1..90
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /inference="alignment:Splign:2.1.0"
     exon            91..2647
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /inference="alignment:Splign:2.1.0"
     CDS             242..2410
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /note="a disintegrin and metalloproteinase domain 21;
                     disintegrin and metalloproteinase domain-containing
                     protein 21; ADAM metallopeptidase domain 21,
                     preproprotein"
                     /codon_start=1
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 21 preproprotein"
                     /protein_id="NP_003804.2"
                     /db_xref="CCDS:CCDS9804.1"
                     /db_xref="GeneID:8747"
                     /db_xref="HGNC:HGNC:200"
                     /db_xref="MIM:603713"
                     /translation="MAVDGTLVYIRVTLLLLWLGVFLSISGYCQAGPSQHFTSPEVVI
                     PLKVISRGRSAKAPGWLSYSLRFGGQKHVVHMRVKKLLVSRHLPVFTYTDDRALLEDQ
                     LFIPDDCYYHGYVEAAPESLVVFSACFGGFRGVLKISGLTYEIEPIRHSATFEHLVYK
                     INSNETQFPAMRCGLTEKEVARQQLEFEEAENSALEPKSAGDWWTHAWFLELVVVVNH
                     DFFIYSQSNISKVQEDVFLVVNIVDSMYKQLGTYIILIGIEIWNQGNVFPMTSIEQVL
                     NDFSQWKQISLSQLQHDAAHMFIKNSLISILGLAYVAGICRPPIDCGVDNFQGDTWSL
                     FANTVAHELGHTLGMQHDEEFCFCGERGCIMNTFRVPAEKFTNCSYADFMKTTLNQGS
                     CLHNPPRLGEIFMLKRCGNGVVEREEQCDCGSVQQCEQDACCLLNCTLRPGAACAFGL
                     CCKDCKFMPSGELCRQEVNECDLPEWCNGTSHQCPEDRYVQDGIPCSDSAYCYQKRCN
                     NHDQHCREIFGKDAKSASQNCYKEINSQGNRFGHCGINGTTYLKCHISDVFCGRVQCE
                     NVRDIPLLQDHFTLQHTHINGVTCWGIDYHLRMNISDIGEVKDGTVCGPGKICIHKKC
                     VSLSVLSHVCLPETCNMKGICNNKHHCHCGYGWSPPYCQHRGYGGSIDSGPASAKRGV
                     FLPLIVIPSLSVLTFLFTVGLLMYLRQCSGPKETKAHSSG"
     regulatory      2620..2625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /note="hexamer: AATAAA"
     polyA_site      2647
                     /gene="ADAM21"
                     /gene_synonym="ADAM 21; ADAM31"
                     /note="major polyA site"
ORIGIN      
        1 gttctgtggc ctcagctaca aaccattgat aaagcatggt ataaaccatc tagacagaaa
       61 tggccatgga aactactgtt tcttctgatg cactgcagtt ctgatctaac tctgccagga
      121 cacagatcca cagggtcagc cctgcatcct gagcagctta gagggagaag ccagaccagc
      181 agtgcctcac accttgtcct cttctgctct ggacagatgg ctccataacg acagcttcat
      241 aatggcagtg gatgggaccc tcgtgtacat cagagtcact cttctgctgc tctggcttgg
      301 ggtatttttg tctatttccg gctactgtca ggctgggccc tcccagcatt tcacttcccc
      361 ggaagtggtg atccccttga aggtgatcag caggggcaga agtgcaaagg ctcctggatg
      421 gctctcctat agtctgcggt ttgggggcca gaaacacgtt gttcatatga gggtcaagaa
      481 gctcttagtt tctagacacc tcccagtgtt cacctacaca gatgaccgtg cactcctgga
      541 ggatcagctc ttcatcccag atgactgtta ctatcatggt tacgtggagg cagcccctga
      601 gtctctggtt gtgttcagtg cttgttttgg gggctttcga ggagtattaa aaataagtgg
      661 cctcacttat gaaattgaac ccatcaggca ctctgccaca tttgaacacc tggtttataa
      721 gataaacagt aatgagacac aattcccagc tatgagatgt ggcttaacag agaaggaagt
      781 agcacgccaa cagttggaat ttgaagaggc tgagaactca gctctggaac caaaatctgc
      841 tggtgactgg tggactcatg catggtttct ggagctagtt gttgtggtga accatgattt
      901 cttcatttac tctcaaagca acatctcaaa ggtgcaagag gatgtatttc ttgttgtcaa
      961 catagtggat tccatgtata agcagttagg tacttacata attttgattg gaattgaaat
     1021 ttggaatcaa ggaaatgttt tcccaatgac aagcatagaa caggtcctga acgatttctc
     1081 tcaatggaaa caaatcagtc tttcccagct acagcatgat gctgcacata tgttcataaa
     1141 aaattcactt ataagtatac ttggcctagc ctatgttgca ggaatatgtc gtccacctat
     1201 tgattgtgga gttgataatt ttcaaggaga tacctggtct ctttttgcca acactgtggc
     1261 ccatgagtta ggtcatacgt taggtatgca gcatgatgaa gaattctgtt tttgtgggga
     1321 aagaggttgc atcatgaata cttttagagt gccagcagag aaattcacca attgcagtta
     1381 cgctgatttt atgaagacca ccttaaacca gggatcatgt ctgcataatc ctccaagatt
     1441 gggggaaatc tttatgctaa agcgctgtgg gaatggtgtg gttgaaagag aagagcagtg
     1501 tgactgtgga tccgtacagc agtgtgaaca agacgcctgt tgtctgttga actgcactct
     1561 aaggcctggg gctgcctgtg cttttgggct ttgttgcaaa gactgcaagt tcatgccatc
     1621 aggggaactc tgtagacaag aggtcaatga atgtgacctt ccagaatggt gcaatggaac
     1681 atctcatcag tgtccagaag atagatatgt gcaggacggg atcccctgta gtgacagtgc
     1741 ctactgctat caaaagaggt gtaataacca tgaccagcat tgcagggaga tttttggtaa
     1801 agatgcaaaa agtgcatctc agaattgcta taaagaaatc aattctcagg gaaaccgttt
     1861 tggtcactgt ggtataaatg gcacaacata cctaaaatgt catatctctg atgtcttttg
     1921 tgggagagtt caatgtgaga atgtgagaga cattcctctt ctccaagatc attttacttt
     1981 gcagcacact catatcaatg gtgtcacctg ctggggtatt gactatcatt taaggatgaa
     2041 catatctgac attggtgaag tgaaagatgg tactgtgtgt ggcccaggaa agatctgcat
     2101 ccataagaag tgtgtcagtc tgtctgtctt gtcacatgtc tgccttcctg agacctgcaa
     2161 tatgaagggg atctgcaata acaaacatca ctgccactgt ggctatgggt ggtccccacc
     2221 ctactgccag cacagaggct atgggggcag tattgacagt ggcccagcat ctgcaaagag
     2281 aggagttttt ttgccgctga ttgtgattcc ttctttgtct gttttgactt tcctgtttac
     2341 tgtcgggctt cttatgtatc tacgacaatg ttctggtccc aaagaaacta aggctcattc
     2401 atcaggttaa gaaaatgtct ctaacttaat attccatgca ttagtacact ttagtctctt
     2461 ggcagtagaa acattagtac atccctgaaa ctgagcacat ttctgaccat ttccagaaag
     2521 ctgcaaagat cttcccttac attagtacca caaacattgt cattaagttc aagttattct
     2581 taacatgttt ctatctattg tttattgttt taagcagcaa ataaagctac atccttccct
     2641 cccttta
//
