LOCUS       NM_001110              11158 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ADAM metallopeptidase domain 10 (ADAM10), transcript
            variant 1, mRNA.
ACCESSION   NM_001110
VERSION     NM_001110.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Dolla,G., Nicolas,S., Dos Santos,L.R., Bourgeois,A.,
            Pardossi-Piquard,R., Bihl,F., Zaghrini,C., Justino,J., Payre,C.,
            Mansuelle,P., Garbers,C., Ronco,P., Checler,F., Lambeau,G. and
            Petit-Paitel,A.
  TITLE     Ectodomain shedding of PLA2R1 is mediated by the metalloproteases
            ADAM10 and ADAM17
  JOURNAL   J Biol Chem 300 (7), 107480 (2024)
   PUBMED   38897568
  REMARK    GeneRIF: Ectodomain shedding of PLA2R1 is mediated by the
            metalloproteases ADAM10 and ADAM17.
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Piwkowska,A., Rachubik,P., Typiak,M., Kulesza,T., Audzeyenka,I.,
            Saleem,M.A., Gruba,N., Wysocka,M., Lesner,A. and Rogacka,D.
  TITLE     ADAM10 as a major activator of reactive oxygen species production
            and klotho shedding in podocytes under diabetic conditions
  JOURNAL   Biochem Pharmacol 225, 116328 (2024)
   PUBMED   38815628
  REMARK    GeneRIF: ADAM10 as a major activator of reactive oxygen species
            production and klotho shedding in podocytes under diabetic
            conditions.
REFERENCE   3  (bases 1 to 11158)
  AUTHORS   Babendreyer,A., Kieselhorst,J., Rinkens,C., Lyashenko,A.M.,
            Dusterhoft,S., Jahr,H., Craveiro,R.B., Wolf,M. and Ludwig,A.
  TITLE     Downregulation of the metalloproteinases ADAM10 or ADAM17 promotes
            osteoclast differentiation
  JOURNAL   Cell Commun Signal 22 (1), 322 (2024)
   PUBMED   38863060
  REMARK    GeneRIF: Downregulation of the metalloproteinases ADAM10 or ADAM17
            promotes osteoclast differentiation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11158)
  AUTHORS   Gao,J.H., He,A.D., Liu,L.M., Zhou,Y.J., Guo,Y.W., Lu,M., Zeng,X.B.,
            Gong,X., Lu,Y.J., Liang,H.F., Zhang,B.X., Ma,R., Zhang,R.Y. and
            Ming,Z.Y.
  TITLE     Direct interaction of platelet with tumor cell aggravates
            hepatocellular carcinoma metastasis by activating
            TLR4/ADAM10/CX3CL1 axis
  JOURNAL   Cancer Lett 585, 216674 (2024)
   PUBMED   38280480
  REMARK    GeneRIF: Direct interaction of platelet with tumor cell aggravates
            hepatocellular carcinoma metastasis by activating
            TLR4/ADAM10/CX3CL1 axis.
REFERENCE   5  (bases 1 to 11158)
  AUTHORS   Drexhage,L.Z., Zhang,S., Dupont,M., Ragaller,F., Sjule,E.,
            Cabezas-Caballero,J., Deimel,L.P., Robertson,H., Russell,R.A.,
            Dushek,O., Sezgin,E., Karaji,N. and Sattentau,Q.J.
  TITLE     Apoptosis-mediated ADAM10 activation removes a mucin barrier
            promoting T cell efferocytosis
  JOURNAL   Nat Commun 15 (1), 541 (2024)
   PUBMED   38225245
  REMARK    GeneRIF: Apoptosis-mediated ADAM10 activation removes a mucin
            barrier promoting T cell efferocytosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11158)
  AUTHORS   Lammich,S., Buell,D., Zilow,S., Ludwig,A.K., Nuscher,B.,
            Lichtenthaler,S.F., Prinzen,C., Fahrenholz,F. and Haass,C.
  TITLE     Expression of the anti-amyloidogenic secretase ADAM10 is suppressed
            by its 5'-untranslated region
  JOURNAL   J Biol Chem 285 (21), 15753-15760 (2010)
   PUBMED   20348102
  REMARK    GeneRIF: the 5'-UTR of ADAM10 may have an important role for
            post-transcriptional regulation of ADAM10 expression and
            consequently Abeta production.
REFERENCE   7  (bases 1 to 11158)
  AUTHORS   McKie,N., Edwards,T., Dallas,D.J., Houghton,A., Stringer,B.,
            Graham,R., Russell,G. and Croucher,P.I.
  TITLE     Expression of members of a novel membrane linked metalloproteinase
            family (ADAM) in human articular chondrocytes
  JOURNAL   Biochem Biophys Res Commun 230 (2), 335-339 (1997)
   PUBMED   9016778
REFERENCE   8  (bases 1 to 11158)
  AUTHORS   Howard,L., Lu,X., Mitchell,S., Griffiths,S. and Glynn,P.
  TITLE     Molecular cloning of MADM: a catalytically active mammalian
            disintegrin-metalloprotease expressed in various cell types
  JOURNAL   Biochem J 317 (Pt 1) (Pt 1), 45-50 (1996)
   PUBMED   8694785
REFERENCE   9  (bases 1 to 11158)
  AUTHORS   Wolfsberg,T.G., Primakoff,P., Myles,D.G. and White,J.M.
  TITLE     ADAM, a novel family of membrane proteins containing A Disintegrin
            And Metalloprotease domain: multipotential functions in cell-cell
            and cell-matrix interactions
  JOURNAL   J Cell Biol 131 (2), 275-278 (1995)
   PUBMED   7593158
  REMARK    Review article
REFERENCE   10 (bases 1 to 11158)
  AUTHORS   O'Bryan,J.P., Fridell,Y.W., Koski,R., Varnum,B. and Liu,E.T.
  TITLE     The transforming receptor tyrosine kinase, Axl, is
            post-translationally regulated by proteolytic cleavage
  JOURNAL   J Biol Chem 270 (2), 551-557 (1995)
   PUBMED   7822279
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA941750.1, AF009615.1,
            BX372217.2, AY726599.1, BE327886.1, AA243020.1 and AC018904.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001110.3.
            
            Summary: Members of the ADAM family are cell surface proteins with
            a unique structure possessing both potential adhesion and protease
            domains. This gene encodes and ADAM family member that cleaves many
            proteins including TNF-alpha and E-cadherin. Alternate splicing
            results in multiple transcript variants encoding different proteins
            that may undergo similar processing. [provided by RefSeq, Feb
            2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF009615.1, SRR14038192.331913.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260408.8/ ENSP00000260408.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-228               DA941750.1         259-486
            229-3089            AF009615.1         525-3385
            3090-3656           BX372217.2         33-599              c
            3657-4382           AY726599.1         65-790
            4383-4730           BE327886.1         50-397              c
            4731-4731           AA243020.1         31-31               c
            4732-4763           BE327886.1         17-48               c
            4764-11158          AC018904.6         133259-139653       c
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.3"
     gene            1..11158
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="ADAM metallopeptidase domain 10"
                     /db_xref="GeneID:102"
                     /db_xref="HGNC:HGNC:188"
                     /db_xref="MIM:602192"
     exon            1..228
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..2420
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /EC_number="3.4.24.81"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; a disintegrin and metalloprotease domain 10;
                     disintegrin and metalloproteinase domain-containing
                     protein 10; kuzbanian protein homolog; mammalian
                     disintegrin-metalloprotease"
                     /codon_start=1
                     /product="disintegrin and metalloproteinase
                     domain-containing protein 10 isoform 1 preproprotein"
                     /protein_id="NP_001101.1"
                     /db_xref="CCDS:CCDS10167.1"
                     /db_xref="GeneID:102"
                     /db_xref="HGNC:HGNC:188"
                     /db_xref="MIM:602192"
                     /translation="MVLLRVLILLLSWAAGMGGQYGNPLNKYIRHYEGLSYNVDSLHQ
                     KHQRAKRAVSHEDQFLRLDFHAHGRHFNLRMKRDTSLFSDEFKVETSNKVLDYDTSHI
                     YTGHIYGEEGSFSHGSVIDGRFEGFIQTRGGTFYVEPAERYIKDRTLPFHSVIYHEDD
                     INYPHKYGPQGGCADHSVFERMRKYQMTGVEEVTQIPQEEHAANGPELLRKKRTTSAE
                     KNTCQLYIQTDHLFFKYYGTREAVIAQISSHVKAIDTIYQTTDFSGIRNISFMVKRIR
                     INTTADEKDPTNPFRFPNIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVG
                     APSGSSGGICEKSKLYSDGKKKSLNTGIITVQNYGSHVPPKVSHITFAHEVGHNFGSP
                     HDSGTECTPGESKNLGQKENGNYIMYARATSGDKLNNNKFSLCSIRNISQVLEKKRNN
                     CFVESGQPICGNGMVEQGEECDCGYSDQCKDECCFDANQPEGRKCKLKPGKQCSPSQG
                     PCCTAQCAFKSKSEKCRDDSDCAREGICNGFTALCPASDPKPNFTDCNRHTQVCINGQ
                     CAGSICEKYGLEECTCASSDGKDDKELCHVCCMKKMDPSTCASTGSVQWSRHFSGRTI
                     TLQPGSPCNDFRGYCDVFMRCRLVDADGPLARLKKAIFSPELYENIAEWIVAHWWAVL
                     LMGIALIMLMAGFIKICSVHTPSSNPKLPPPKPLPGTLKRRRPPQPIQQPQRQRPRES
                     YQMGHMRR"
     exon            229..379
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            380..498
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            499..657
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            658..758
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            759..908
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1001
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1185
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1349
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1533
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1684
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1868
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..1977
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2198
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2325
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..11158
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4556..4561
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: AATAGA"
     polyA_site      4573
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="major polyA site"
     regulatory      4740..4745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: AATAAA"
     polyA_site      4763
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
     regulatory      5335..5340
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: AATAAA"
     polyA_site      5359
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
     regulatory      8655..8660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: ATTAAA"
     polyA_site      8679
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
     regulatory      8955..8960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: AATAAA"
     polyA_site      8975
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
     regulatory      11140..11145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
                     /note="hexamer: AATAAA"
     polyA_site      11158
                     /gene="ADAM10"
                     /gene_synonym="AD10; AD18; CD156c; CDw156; HsT18717; kuz;
                     MADM; RAK"
ORIGIN      
        1 gttgccggcc cctgaagtgg agcgagaggg aggtgcttcg ccgtttctcc tgccagggga
       61 ggtcccggct tcccgtggag gctccggacc aagccccttc agcttctccc tccggatcga
      121 tgtgctgctg ttaacccgtg aggaggcggc ggcggcggca gcggcagcgg aagatggtgt
      181 tgctgagagt gttaattctg ctcctctcct gggcggcggg gatgggaggt cagtatggga
      241 atcctttaaa taaatatatc agacattatg aaggattatc ttacaatgtg gattcattac
      301 accaaaaaca ccagcgtgcc aaaagagcag tctcacatga agaccaattt ttacgtctag
      361 atttccatgc ccatggaaga catttcaacc tacgaatgaa gagggacact tcccttttca
      421 gtgatgaatt taaagtagaa acatcaaata aagtacttga ttatgatacc tctcatattt
      481 acactggaca tatttatggt gaagaaggaa gttttagcca tgggtctgtt attgatggaa
      541 gatttgaagg attcatccag actcgtggtg gcacatttta tgttgagcca gcagagagat
      601 atattaaaga ccgaactctg ccatttcact ctgtcattta tcatgaagat gatattaact
      661 atccccataa atacggtcct caggggggct gtgcagatca ttcagtattt gaaagaatga
      721 ggaaatacca gatgactggt gtagaggaag taacacagat acctcaagaa gaacatgctg
      781 ctaatggtcc agaacttctg aggaaaaaac gtacaacttc agctgaaaaa aatacttgtc
      841 agctttatat tcagactgat catttgttct ttaaatatta cggaacacga gaagctgtga
      901 ttgcccagat atccagtcat gttaaagcga ttgatacaat ttaccagacc acagacttct
      961 ccggaatccg taacatcagt ttcatggtga aacgcataag aatcaataca actgctgatg
     1021 agaaggaccc tacaaatcct ttccgtttcc caaatattgg tgtggagaag tttctggaat
     1081 tgaattctga gcagaatcat gatgactact gtttggccta tgtcttcaca gaccgagatt
     1141 ttgatgatgg cgtacttggt ctggcttggg ttggagcacc ttcaggaagc tctggaggaa
     1201 tatgtgaaaa aagtaaactc tattcagatg gtaagaagaa gtccttaaac actggaatta
     1261 ttactgttca gaactatggg tctcatgtac ctcccaaagt ctctcacatt acttttgctc
     1321 acgaagttgg acataacttt ggatccccac atgattctgg aacagagtgc acaccaggag
     1381 aatctaagaa tttgggtcaa aaagaaaatg gcaattacat catgtatgca agagcaacat
     1441 ctggggacaa acttaacaac aataaattct cactctgtag tattagaaat ataagccaag
     1501 ttcttgagaa gaagagaaac aactgttttg ttgaatctgg ccaacctatt tgtggaaatg
     1561 gaatggtaga acaaggtgaa gaatgtgatt gtggctatag tgaccagtgt aaagatgaat
     1621 gctgcttcga tgcaaatcaa ccagagggaa gaaaatgcaa actgaaacct gggaaacagt
     1681 gcagtccaag tcaaggtcct tgttgtacag cacagtgtgc attcaagtca aagtctgaga
     1741 agtgtcggga tgattcagac tgtgcaaggg aaggaatatg taatggcttc acagctctct
     1801 gcccagcatc tgaccctaaa ccaaacttca cagactgtaa taggcataca caagtgtgca
     1861 ttaatgggca atgtgcaggt tctatctgtg agaaatatgg cttagaggag tgtacgtgtg
     1921 ccagttctga tggcaaagat gataaagaat tatgccatgt atgctgtatg aagaaaatgg
     1981 acccatcaac ttgtgccagt acagggtctg tgcagtggag taggcacttc agtggtcgaa
     2041 ccatcaccct gcaacctgga tccccttgca acgattttag aggttactgt gatgttttca
     2101 tgcggtgcag attagtagat gctgatggtc ctctagctag gcttaaaaaa gcaattttta
     2161 gtccagagct ctatgaaaac attgctgaat ggattgtggc tcattggtgg gcagtattac
     2221 ttatgggaat tgctctgatc atgctaatgg ctggatttat taagatatgc agtgttcata
     2281 ctccaagtag taatccaaag ttgcctcctc ctaaaccact tccaggcact ttaaagagga
     2341 ggagacctcc acagcccatt cagcaacccc agcgtcagcg gccccgagag agttatcaaa
     2401 tgggacacat gagacgctaa ctgcagcttt tgccttggtt cttcctagtg cctacaatgg
     2461 gaaaacttca ctccaaagag aaacctatta agtcatcatc tccaaactaa accctcacaa
     2521 gtaacagttg aagaaaaaat ggcaagagat catatcctca gaccaggtgg aattacttaa
     2581 attttaaagc ctgaaaattc caatttgggg gtgggaggtg gaaaaggaac ccaattttct
     2641 tatgaacaga tatttttaac ttaatggcac aaagtcttag aatattatta tgtgccccgt
     2701 gttccctgtt cttcgttgct gcattttctt cacttgcagg caaacttggc tctcaataaa
     2761 cttttaccac aaattgaaat aaatatattt ttttcaactg ccaatcaagg ctaggaggct
     2821 cgaccacctc aacattggag acatcacttg ccaatgtaca taccttgtta tatgcagaca
     2881 tgtatttctt acgtacactg tacttctgtg tgcaattgta aacagaaatt gcaatatgga
     2941 tgtttctttg tattataaaa tttttccgct cttaattaaa aattactgtt taattgacat
     3001 actcaggata acagagaatg gtggtattca gtggtccagg attctgtaat gctttacaca
     3061 ggcagttttg aaatgaaaat caatttacct ttctgttacg atggagttgg ttttgatact
     3121 cattttttct ttatcacatg gctgctacgg gcacaagtga ctatactgaa gaacacagtt
     3181 aagtgttgtg caaactggac atagcagcac atactacttc agagttcatg atgtagatgt
     3241 ctggtttctg cttacgtctt ttaaactttc taattcaatt ccatttttca attaataggt
     3301 gaaattttat tcatgctttg atagaaatta tgtcaatgaa atgattcttt ttatttgtag
     3361 cctacttatt tgtgtttttc atatatctga aatatgctaa ttatgttttc tgtctgatat
     3421 ggaaaagaaa agctgtgtct ttatcaaaat atttaaacgg ttttttcagc atatcatcac
     3481 tgatcattgg taaccactaa agatgagtaa tttgcttaag tagtagttaa aattgtagat
     3541 aggccttctg acattttttt tcctaaaatt tttaacagca ttgaaggtga aacagcacaa
     3601 tgtcccattc caaatttatt tttgaaacag atgtaaataa ttggcatttt aaagagaaag
     3661 caaaaacatt taatgtatta acaggcttat tgctatgcag gaaatagaag gggcattaca
     3721 aaaattgaag cttgtgacat atttattgct tctgttttcc aactacatca cttcaactag
     3781 aagtaaagct atgattttcc tgacttcaca taggaggcaa atttagagaa agttgtaaag
     3841 atttctatgt tttgggtttt tttttttcct tttttttttt aagagtataa ggtttacaca
     3901 atcattctca taatgtgacg caagccagca aggccaaaaa tgctagagaa aataacggga
     3961 tctcttcctt gtaaacttgt acagtatgtg gtgacttttt caaaatacag ctttttgtac
     4021 atgatttaga gacaaatttt gtacatgaaa ccccagatag actataaata attctaaaca
     4081 aacaagtagg tagatatgta tgtaattgct tttaaatcat ttaaatgcct ttgtttttgg
     4141 actgtgcaaa ggttggaagt gggtttgcat ttctaaaatg gtgactttta ttctgcaaga
     4201 gttcttagta acttcttgag tgtggtagac tttggaacat gtaaattttt tgcttgtaat
     4261 gttatcctgt ggtaggattt tggcaggtac acacactgcc ctattttatt ttgagtctaa
     4321 gttaaatgtt ttctgaaaag agatacatgc actgaactct ttccactgcg aatcaagatg
     4381 tggtaatata aaaggatcaa gacaaatgag atctaatact actgtcagtt ttaatgtcca
     4441 ctgtgtttta tacagtatct ttttttgttc actttggaaa tttttactaa aaattgcaaa
     4501 aaataaagta ttgtgcaaag atgtaaggtt ttttgaaact tgaaatgcat taataaatag
     4561 acgattaaat caacttgaag gttctatact ctttgaactc tgagaactat cacaagaagc
     4621 ttcccacaag gcagtgtttt cttacagttg tctcttccta caaaagtata gattatcttt
     4681 attcttaata ctttggaatc catgtagaaa atttccagtt agatactctg cgtacacaca
     4741 ataaaccttt ttaaaacacc caactaatct caactgcatt acattgtttc taatcaatat
     4801 tcagtgcttg tcttggtgga agaggtgagt cattttgaaa acttatggtc ttgtttttat
     4861 gtgtttttca aagttttgaa tgctaagtac ctcatttatt ttaaaaagcc tagtttaatg
     4921 ataagtttgt ttaaaatttt gagccatcat ttttctcttc atagcaaata aggagagaat
     4981 tgacatttca gtgttaccta gaaaaggaat tgtaagccca gaataattcc ctgcatgagg
     5041 taatctgctt caaattcttt ttttagtcaa ggttagctat aagtaatact tgttaaatga
     5101 gtaaatatgt aatactttgt gaattacttt gttaatttag gagcatcaaa tgtatattat
     5161 gtttagttat ttatgaaact ctcaatattg attgatttgg gtaattataa attagttatt
     5221 tttacttgta attgaatgct taaattctgt ttacagtccg tcctctctcc ctccatccct
     5281 ccctccccag ttttataaat tcaggtacca attcacaaac aaaatcagaa ataaaataaa
     5341 tttattgact gcttctggat ttagcattcc ctgtagtgtc aagcaatgtc atgcagtttg
     5401 gggaagcatt tatttaagga aatgacaact ttctctgatc agtcttgttt tgtgaggtgt
     5461 cttcaacact ttatgctttg ggtacttcgt gtttgtcaca gtcttaggat agtgaaatct
     5521 gatttgtcca agcggagcaa actactcgac cctcagtcct tgtatttgtc cctgtagtaa
     5581 gacctaatta ttattatttc ttaaagatgg gattggtgtc cttggcaact atgaaatttc
     5641 ggggcttgtg catgagaagg catttcttat taagtatttc taattgaagg tatcagagtg
     5701 tcaagcatta caaacctgga cagttcacct ggaggagtac aagaagagat attcattatc
     5761 catatttaaa gggtcaaggt ttcccaaaac cagggtgcaa gccagatgta gttttaaagc
     5821 agctgccagg gacagttcat ctttagagaa gtcactaaag ttgtaagaaa ttttagtttc
     5881 cccaaaacca ctttcaactt cttagaaact agaaagacaa ttggtttgcc ccacagagga
     5941 caacttcagt ttcagcatct ctcatgttgt gttcttgatt aaaaacaact tccatttgat
     6001 atacttttcc gtttattacc agtttagttt tttcactatt gtttctgtat tcaactcttt
     6061 atatgattag gatagaaatt tagcccttct gttttatatt actatattgt ttgtgtgtct
     6121 tagatatata catgtatgta ctattttcag tagaaattca tgtattttat aattggtaag
     6181 ttcttcagag catctcttct ataaaaagca acaggatgct aggtaaaacg gagcattgag
     6241 caaaatactg attagttttt gctttttcct gaaatctaca ctaaagtgat agggtgtggg
     6301 gtaatccaac aaggacaagg tgaattgaac aagaacgaaa tctggaagca gatgaaggag
     6361 tactattgat tgggcagacc cagggaagtc aaatcctaaa ccagcagtgg gaacacaaca
     6421 gaatggtgta gtttgcactg gtaagatttg ggtacctggc agggctgggt gcggtggctc
     6481 acacctgtaa tcccagcact ttgggaggcc aaggcgggtg gatcacttga ggtcaggagt
     6541 tcgagaccac cctggccaac atggtgaaac cccgtctcta ctaaaaatac agctgggcgt
     6601 ggtggcacat gcttgtaatc ccagctactc gggaggcaga ggcaggagat ttgcttgaac
     6661 ccgggaggca gaggcaggag atttgcttga acccaggagg cagaggctgc agtgagccgc
     6721 gattgcgcca ttgcactcca gcctgggtga cagagcgaga ctctgtctca aaaaaaaaaa
     6781 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaattt gagtacctgg cctttgttac
     6841 tttttttcta tgtgtgtgac aaaaacataa tatgcacact tttgtaaccc acctttctca
     6901 tttaatggta cattgataat gtatatcaca ttaactactc taaatatttc tgtggatgta
     6961 tgtttttttt tttcttaacc aatttcccat tgtttggaca tgtaggttcc acattgttta
     7021 ttattttaaa caattctaaa gaattttaaa caattcttag gaaaatcctc agcctaataa
     7081 tgaaattaat tcctaaaagt ggaattgttg gggtaaaggt tttttgaggg acattgataa
     7141 aaattatggt actgtctccc agatagatgt accaagttat actaccacga tttaatatat
     7201 atatatatat atattaaatc agagtcccat ccttagaaat ccacatatat gcagccacat
     7261 gaatgtatta gaaacaataa tagaagactc atgcttaatt cagttgatta gctttagaca
     7321 taattcaaat gcaagtcaaa ttgagtgccc taattgtggt ctcttaagta ccatttttct
     7381 tcaagggaac cagactcctt tggataaatc actaattcca cctgtaagaa agaaatgtac
     7441 aagaagaacc taggaaacat tgttttgtac cagatcagaa agattcagga ggcaccttag
     7501 aagttaccac tggccaaggc tgagataact ttagcatcag caaggataat atctgcaaga
     7561 gattgaaact catagtattg tatttaactc tgtgagttaa tgatggtagt ggacagaatt
     7621 atagttacct ttgggatacg cttttaaaga aattccaggt aataagagaa atgatagaat
     7681 taggatatca ccattttacc cccccaacaa tttatggatc tagacaataa tcgccagtga
     7741 ctgctaacct cacaaagtga gagcaatcag attttgtgcc tcctaatgga agtacatata
     7801 ccacctatga agcagttctg ccaaaagtca catctcatca tgatgaagcc tcctgatcta
     7861 actacccctt cattagaaat acaggggaca gagggacaaa taatatacaa gggactcaat
     7921 cagcaaaatc cagactctgg aaaactacaa gacatatggt cctgcttcaa caacagaaat
     7981 gcaaagagaa aagacaacga tgggttaaag gagacttaag agctacatct atcaagacaa
     8041 tttatggact tatttggata ctgatttgaa caaactgttg agaccattgg aaaaatgtga
     8101 aaagtggata tttgatatta aggtttttaa ttatttttag gtgtgataat ggtattgtta
     8161 cattttttaa aggacccctt ttagagatgc aaattgaaac acttaaaaaa tgaaatgata
     8221 cgatgtataa gtttttgctt aaaaataagg attgaagttg gctggtgtgt gtggatatag
     8281 ttgaaacaag attggctgtg agttgataat tattgaagct gggtgatggg cacttgggga
     8341 tttattatac tattttctct acctgtgttt atatttgaaa tttttcatag aagttttaaa
     8401 atgtggccag ttgtgatggc tcatacctgt aatcccaaca ctttgggagg ccaaggtggg
     8461 aggatcactt gagctcagga gttagagacc agcctgggca aaatagtgag actccatctc
     8521 aaagaaaaaa aaaaagtgtt ttaaatgtga atcaaattcc tatagaagct gattcattac
     8581 tgtttttatt ttagcagtaa ttcatgataa tgacctgtat tcataatgat tttcataatg
     8641 attgttttag tggaattaaa cttgaaccag tcaagctaac ataattatat tctgctccag
     8701 ttacaatgaa taattaattg atttcaactg ctagggtgaa ctcttgaagc tatcagtcat
     8761 ccagcaatct tagcaagcag gccattgggt ccctgtttgc tctgtctctc tctctctctc
     8821 tcactgttga agggcttagc taactactta agtaaaatat ttgttctctg ttaaacatgt
     8881 caaggagtat ggtcagctta tccacattaa gcctgtgtgt cccacgttgg agtaaatgtt
     8941 aagtagctca ctacaataaa ctagattctt ctgccctctc ttgtttaaat gatcatgttc
     9001 cctggaggtg gaaatagatc tttaaaaaga tattctgtag ttgtttgttc tcagtgtaaa
     9061 aaaatgagaa taatttgata agagtgtagg ttgtcttata taaaaagtgg ttccatttgc
     9121 atgaatttta gaaaaatcat tttggaaaaa tgaaggctat gtggttatac tgaacacatt
     9181 aagcaatttt attctttatt ttaaatgaat attttattat cgttttcttc ccttgccctt
     9241 tgggtatggg agttagcctt tgtgtttcta aatacaacag gccggttttt ataaattaag
     9301 gtgtcaatat attcttcatt atttagtttt gtgattgtgg ttagttttca tttttcttaa
     9361 gtatctgcta gtagcatctg taattaagtg aagtgacctg ttaaccattt tcctctttct
     9421 cctcctttcc tcctccttga aacatatcag agcatgtttg aaattctttg gcttttatgg
     9481 tatgcatttg ctgatatgca ttgaccagtt accttactca cagatacttc ttaggcactt
     9541 gattgtgcca gggccttggc tagatgataa gaatacagta gtgaacttaa cagtttccct
     9601 gccctggtga agcgtatggt cttgtaggtg agatagatat cagataatca tgtgaataaa
     9661 tgtacaattc cagctgtgat acatgctgag gaggaggttt ttggtgatcc aagagctgat
     9721 catgcagaga taggactgag aaaggagggt gggacgttgt cacagctgat aatgcagaga
     9781 taggactgag aaaggagggt gggacatcag gaaggtcaga gaattcctta tgaaagtgat
     9841 gcttgagtca aaatatgatg gatgaagaga gtttaaatag attacataga atttttaata
     9901 atgtcgattg gttatatact gggcactgat agctgatttt tctttgggga aaggtatgtc
     9961 agcctagtca ttcagattcc tttatttttt taaatgtttt ttcatttttt gctttgcatt
    10021 gcattcattt gctgaagagc tggcttgtac tttggcaggt gtcatacttg gttattctcc
    10081 ttaggatatt ggcccaacaa tctgggagtt gtgaaaggcg cttcgctttt cagacctggg
    10141 cgtctgtatc atgactatca taaatttagg attaagacac ctagcctcct accaggatga
    10201 atgaggtgtc catgtgacct gctgtgccct ggaattttat acatctttct ctcatagcac
    10261 acaccatatt acaatataat cctgcctcat ctaagccaaa ctttcgagag aatcatttac
    10321 actcagtggc tacttcagct cccattcact tatcaacctg ctgcaatttt tcacagcccc
    10381 caaaggactg cagtctgtgc cttcagggag ctgagggtct agcggaagga aagaaaccag
    10441 cagttacagt acagaggggt ttgtgttgga aactctacaa acacaggatg ccctggtagc
    10501 tcagaggaag tgcatatcga gcatggtagg taggtagtgg gaagagccaa gatgacttcc
    10561 cagaggagaa aagctggacc tgagttttgg agtttcggta aaagtttgct ctaactagtc
    10621 caagctgctg tcacaagctt ttagaaatga tgtaaccatg gggcagttga ctgtcgtcat
    10681 gttctttgct attttcatga ctctggatgt gcttttccta ttccctggat tgccctttcc
    10741 ctcgattcct ctgcaggact gggctttatt aatctccatt tccttgagct tggctatagt
    10801 aggtgttcaa taaacatttg ttttgttgtg tgctttgtaa ataggcaatg aagctgattt
    10861 cacaagatag gcacaaaagt tagtttcatt acaacacatt accaacagct gtatttttaa
    10921 cttttaacat atctcattct aaatcctgtg gcagcacaac ctccttccgt catacctgga
    10981 gataaatttt ctttcaaaat ctaatatgca ctgtatttat agaatatgaa acataccgac
    11041 catgttttgc aaaaatggga aaggcataac ttagctttgg ggcatgtaag taacaactcc
    11101 tgataggaga agaaatgtat tcagaaagct caaattagaa ataaaatggg agactcta
//
