LOCUS       NM_005722               3783 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens actin related protein 2 (ACTR2), transcript variant 2,
            mRNA.
ACCESSION   NM_005722
VERSION     NM_005722.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Hao,P., Qu,Q., Pang,Z., Li,L., Du,S., Shang,L., Jin,C., Xu,W.,
            Ha,Z., Jiang,Y., Chen,J., Gao,Z., Jin,N., Wang,J. and Li,C.
  TITLE     Interaction of species A rotavirus VP4 with the cellular proteins
            vimentin and actin related protein 2 discovered by a proximity
            interactome assay
  JOURNAL   J Virol 97 (12), e0137623 (2023)
   PUBMED   37991368
  REMARK    GeneRIF: Interaction of species A rotavirus VP4 with the cellular
            proteins vimentin and actin related protein 2 discovered by a
            proximity interactome assay.
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   van Eeuwen,T., Boczkowska,M., Rebowski,G., Carman,P.J.,
            Fregoso,F.E. and Dominguez,R.
  TITLE     Transition State of Arp2/3 Complex Activation by Actin-Bound
            Dimeric Nucleation-Promoting Factor
  JOURNAL   Proc Natl Acad Sci U S A 120 (33), e2306165120 (2023)
   PUBMED   37549294
  REMARK    GeneRIF: Transition State of Arp2/3 Complex Activation by
            Actin-Bound Dimeric Nucleation-Promoting Factor.
REFERENCE   3  (bases 1 to 3783)
  AUTHORS   Sadhu,L., Tsopoulidis,N., Hasanuzzaman,M., Laketa,V., Way,M. and
            Fackler,O.T.
  TITLE     ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP
            drive distinct Arp2/3-dependent actin remodeling events in CD4 T
            cells
  JOURNAL   Elife 12, e82450 (2023)
   PUBMED   37162507
  REMARK    GeneRIF: ARPC5 isoforms and their regulation by
            calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin
            remodeling events in CD4 T cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3783)
  AUTHORS   Tabata,K., Ishiyama,A., Nakamura,Y., Sasaki,M., Inoue,K. and
            Goto,Y.I.
  TITLE     A familial 2p14 microdeletion disrupting actin-related protein 2
            and Ras-related protein Rab-1A genes with intellectual disability
            and language impairment
  JOURNAL   Eur J Med Genet 65 (3), 104446 (2022)
   PUBMED   35134569
  REMARK    GeneRIF: A familial 2p14 microdeletion disrupting actin-related
            protein 2 and Ras-related protein Rab-1A genes with intellectual
            disability and language impairment.
REFERENCE   5  (bases 1 to 3783)
  AUTHORS   Zhang,L., Zhang,Y., Lei,Y., Wei,Z., Li,Y., Wang,Y., Bu,Y. and
            Zhang,C.
  TITLE     Proline-rich 11 (PRR11) drives F-actin assembly by recruiting the
            actin-related protein 2/3 complex in human non-small cell lung
            carcinoma
  JOURNAL   J Biol Chem 295 (16), 5335-5349 (2020)
   PUBMED   32169900
  REMARK    GeneRIF: Proline-rich 11 (PRR11) drives F-actin assembly by
            recruiting the actin-related protein 2/3 complex in human non-small
            cell lung carcinoma.
REFERENCE   6  (bases 1 to 3783)
  AUTHORS   Machesky,L.M. and Insall,R.H.
  TITLE     Scar1 and the related Wiskott-Aldrich syndrome protein, WASP,
            regulate the actin cytoskeleton through the Arp2/3 complex
  JOURNAL   Curr Biol 8 (25), 1347-1356 (1998)
   PUBMED   9889097
REFERENCE   7  (bases 1 to 3783)
  AUTHORS   Machesky,L.M., Reeves,E., Wientjes,F., Mattheyse,F.J., Grogan,A.,
            Totty,N.F., Burlingame,A.L., Hsuan,J.J. and Segal,A.W.
  TITLE     Mammalian actin-related protein 2/3 complex localizes to regions of
            lamellipodial protrusion and is composed of evolutionarily
            conserved proteins
  JOURNAL   Biochem J 328 (Pt 1) (Pt 1), 105-112 (1997)
   PUBMED   9359840
REFERENCE   8  (bases 1 to 3783)
  AUTHORS   Welch,M.D., DePace,A.H., Verma,S., Iwamatsu,A. and Mitchison,T.J.
  TITLE     The human Arp2/3 complex is composed of evolutionarily conserved
            subunits and is localized to cellular regions of dynamic actin
            filament assembly
  JOURNAL   J Cell Biol 138 (2), 375-384 (1997)
   PUBMED   9230079
REFERENCE   9  (bases 1 to 3783)
  AUTHORS   Winter,D., Podtelejnikov,A.V., Mann,M. and Li,R.
  TITLE     The complex containing actin-related proteins Arp2 and Arp3 is
            required for the motility and integrity of yeast actin patches
  JOURNAL   Curr Biol 7 (7), 519-529 (1997)
   PUBMED   9210376
  REMARK    Erratum:[Curr Biol 1997 Sep 1;7(9):R593]
REFERENCE   10 (bases 1 to 3783)
  AUTHORS   Welch,M.D., Iwamatsu,A. and Mitchison,T.J.
  TITLE     Actin polymerization is induced by Arp2/3 protein complex at the
            surface of Listeria monocytogenes
  JOURNAL   Nature 385 (6613), 265-269 (1997)
   PUBMED   9000076
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC404298.1, AF006082.1,
            AC007318.4 and W15444.1.
            
            On Feb 27, 2020 this sequence version replaced NM_005722.3.
            
            Summary: The specific function of this gene has not yet been
            determined; however, the protein it encodes is known to be a major
            constituent of the ARP2/3 complex. This complex is located at the
            cell surface and is essential to cell shape and motility through
            lamellipodial actin assembly and protrusion. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (b) has the same
            N- and C-termini but is shorter compared to isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            transcript and genomic sequence to make the sequence consistent
            with the reference genome assembly. The genomic coordinates used
            for the transcript record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR4643866.14125.1,
                                           ERR4643844.44661.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260641.10/ ENSP00000260641.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               DC404298.1         137-263
            128-1093            AF006082.1         123-1088
            1094-3736           AC007318.4         190208-192850
            3737-3783           W15444.1           11-57               c
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p14"
     gene            1..3783
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /note="actin related protein 2"
                     /db_xref="GeneID:10097"
                     /db_xref="HGNC:HGNC:169"
                     /db_xref="MIM:604221"
     exon            1..127
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..1264
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /note="isoform b is encoded by transcript variant 2;
                     actin-like protein 2; ARP2 actin related protein 2
                     homolog"
                     /codon_start=1
                     /product="actin-related protein 2 isoform b"
                     /protein_id="NP_005713.1"
                     /db_xref="CCDS:CCDS1881.1"
                     /db_xref="GeneID:10097"
                     /db_xref="HGNC:HGNC:169"
                     /db_xref="MIM:604221"
                     /translation="MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRST
                     TKVGNIEIKDLMVGDEASELRSMLEVNYPMENGIVRNWDDMKHLWDYTFGPEKLNIDT
                     RNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAIQAVLTLYAQGLLTGVVVDSG
                     DGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRMIKE
                     KLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGV
                     GVAELLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDV
                     EKLSKFKIRIEDPPRRKHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVT
                     VR"
     exon            128..238
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            239..454
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            455..527
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            528..664
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..814
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..960
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1093
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..3783
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3757..3762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /note="hexamer: AATAAA"
     polyA_site      3783
                     /gene="ACTR2"
                     /gene_synonym="ARP2"
                     /note="major polyA site"
ORIGIN      
        1 gcaagaggaa gaagagaaaa cggccgggcg gcggtggctg taggttgtgc ggctgcagcg
       61 gctcttccct gggcggacga tggacagcca gggcaggaag gtggtggtgt gcgacaacgg
      121 caccgggttt gtgaagtgtg gatatgcagg ctctaacttt ccagaacaca tcttcccagc
      181 tttggttgga agacctatta tcagatcaac caccaaagtg ggaaacattg aaatcaagga
      241 tcttatggtt ggtgatgagg caagtgaatt acgatcaatg ttagaagtta actaccctat
      301 ggaaaatggc atagtacgaa attgggatga catgaaacac ctgtgggact acacatttgg
      361 accagagaaa cttaatatag ataccagaaa ttgtaaaatc ttactcacag aacctcctat
      421 gaacccaacc aaaaacagag agaagattgt agaggtaatg tttgaaactt accagttttc
      481 cggtgtatat gtagccatcc aggcagttct gactttgtac gctcaaggtt tattgactgg
      541 tgtagtggta gactctggag atggtgtgac tcacatttgc ccagtatatg aaggcttttc
      601 tctccctcat cttaccagga gactggatat tgctgggagg gatataacta gatatcttat
      661 caagctactt ctgttgcgag gatacgcctt caaccactct gctgattttg aaacggttcg
      721 catgattaaa gaaaaactgt gttacgtggg atataatatt gagcaagagc agaaactggc
      781 cttagaaacc acagtattag ttgaatctta tacactccca gatggacgta tcatcaaagt
      841 tgggggagag agatttgaag caccagaagc tttatttcag cctcacttga tcaatgttga
      901 aggagttggt gttgctgaat tgctttttaa cacaattcag gcagctgaca ttgataccag
      961 atctgaattc tacaaacaca ttgtgctttc tggagggtct actatgtatc ctggcctgcc
     1021 atcacggttg gaacgagaac ttaaacagct ttacttagaa cgagttttga agggtgatgt
     1081 ggaaaaactt tctaaattta agatccgcat tgaagaccca ccccgcagaa agcacatggt
     1141 attcctgggt ggtgcagttc tagcggatat catgaaagac aaagacaact tttggatgac
     1201 ccgacaagag taccaagaaa agggtgtccg tgtgctagag aaacttggtg tgactgttcg
     1261 ataaactcca aagcttgttc ccgtcatacc cgtaatgctt tcttttttcc tttattgcca
     1321 atctttgaac tcattcaact ccaggacatg gaagaggcct ctctctgccc tttgactgga
     1381 aaggtcaagt tttattctgg tgtcttgggg aagctttgtt aaatttttgt taatgtgggt
     1441 aaatctgagt ttaattcaac tgcttcccta catagactag agggctaagg attctgtctg
     1501 ctgctttgtt tcttctaagt aggcatttag atcattccta taggcttcct attttcactt
     1561 tactgctcta atgctgctag tcgtagtctt tagcacacta ggtggtatgc ctttattagc
     1621 ataaaacaaa aaaaacttta acaggagctt ttacatatta ctgggatggg gggtggttcg
     1681 ggatgggtgg gcagctgctg aaccctttag ggcatttcct ctgtaatgtg gcgctttcaa
     1741 ctgtactgct gcagctttaa gtaccttaaa gcttctcctg tgaacttctt agggaaatgt
     1801 taggttcaga actaaagtgt tttgggtggg ttttgttgcg ggggggaggg taacaatggg
     1861 tggtcttctg atttttattt ttgaggtttt gtcaactgga gtacgtagag gaactttatt
     1921 tacagtactt tgatttggca ggttttcttc tacttgtgct ctgcctggag ctgtttccat
     1981 atgatataaa aagcaagtgt agtattccat tactatgtgg cttagggatt tatttgtttt
     2041 ttaaaatcaa ccatgttagc tgggattaga ctccctacag tccttcaatg gaaaagtaac
     2101 atttaaaaat cctttgggta attcgaatta cagatttaaa agagcttaag atctggtgtt
     2161 ttgttaatgc ttctgtttat tccagaagca ttaaggtaac ccattgccaa gtatcattct
     2221 tgcaaattat tcttttatat aactgaccag tgcttaataa aacaagcagg tacttacaaa
     2281 taattactgg cagtaggtta taattggtgg tttaaaaata acattggaat acaggacttg
     2341 ttgccaattg ggtaattttc attagttgtt ttgtttgttt tgatttgaaa cctggaaata
     2401 cagtaaaatt tgactgttta aaatgttggc caaaaaaatc aagatttaat ttttttattt
     2461 gtactgaaaa actaatcata actgttaatt ctcagccatc tttgaagctt gaaagaagag
     2521 tctttggtat tttgtaaacg ttagcagact ttcctgccag tgtcagaaaa tcctatttat
     2581 gaatcctgtc ggtattcctt ggtatctgaa aaaaatacca aatagtacca tacatgagtt
     2641 atttctaagt ttgaaaaata aaaagaaatt gcatcacact aattacaaaa tacaagttct
     2701 ggaaaaaata tttttcttca ttttaaaact tttttttaac taataatagc tttgaaagaa
     2761 gaggcttaat ttgggggtgg taactaaaat caaaagaaat gattgacttg agggtctctg
     2821 tttggtaaga atacatcatt agcttaaata agcagcagaa ggttagtttt aattatgtag
     2881 cttctgttaa tattaagtgt tttttgtctg ttttacctca atttgaacag ataagtttgc
     2941 ctgcatgctg gacatgcctc agaaccctga atagcccgta ctagatcttg ggaacatgga
     3001 tcttagagtc actttggaat aagttcttat ataaataccc ccagcctttt gagaacgggg
     3061 cttgttaaag gacgcgtatg tagggcccgt acctactggc agttgggttc agggaaatgg
     3121 gattgacttg gccttcaggc tcctttggtc ataattttaa aatatgggag tagaaaacaa
     3181 caaagaatgg aatggactct taaaacaatg aaagagcatt tatcgtttgt cccttgaatg
     3241 tagaatttgt ttttgatttc ataattctgc tggtaaatgt gacagttaaa atggtgcatt
     3301 atgtatatat attataattt agaaatacca ttttataatt ttactattcc agggtgacat
     3361 aatgcattta aatttgggat ttgggtggag tattatgttt aactggagtt gtcaagtatg
     3421 agtccctcag gaaaaaaaaa aaattctgtt ttaaaaagca atctgattct tagctcttga
     3481 aactattgct acttaaattt ccaataatta aaaatttaaa atttttaaat tagaattgcc
     3541 aatacttcta catttgagaa gggttttttt agaaatacat ttagtaaagt ccccaagaca
     3601 ttagtcttac atttaaactt ttttctttaa aacatggttt tggtggttaa cttttacaca
     3661 gttctgagta ctgttaatat ctggaaagta tcttgagata tcagtggaaa gctaaacagt
     3721 ctaaattaac atgaaatact tcattttgat tgagaaaata aaatcagatt ttttcaaagt
     3781 caa
//
