LOCUS       NM_001103               4872 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens actinin alpha 2 (ACTN2), transcript variant 1, mRNA.
ACCESSION   NM_001103
VERSION     NM_001103.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Wang,K., Wang,Y., Wan,H., Wang,J., Hu,L., Huang,S., Sheng,M.,
            Wu,J., Han,X., Yu,Y., Chen,P. and Chen,F.
  TITLE     Actn2 defects accelerates H9c2 hypertrophy via ERK phosphorylation
            under chronic stress
  JOURNAL   Genes Genomics 46 (9), 1013-1022 (2024)
   PUBMED   38990270
  REMARK    GeneRIF: Actn2 defects accelerates H9c2 hypertrophy via ERK
            phosphorylation under chronic stress.
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Donkervoort,S., Mohassel,P., O'Leary,M., Bonner,D.E., Hartley,T.,
            Acquaye,N., Brull,A., Mozaffar,T., Saporta,M.A., Dyment,D.A.,
            Sampson,J.B., Pajusalu,S., Austin-Tse,C., Hurth,K., Cohen,J.S.,
            McWalter,K., Warman-Chardon,J., Crunk,A., Foley,A.R., Mammen,A.L.,
            Wheeler,M.T., O'Donnell-Luria,A. and Bonnemann,C.G.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     Recurring homozygous ACTN2 variant (p.Arg506Gly) causes a recessive
            myopathy
  JOURNAL   Ann Clin Transl Neurol 11 (3), 629-640 (2024)
   PUBMED   38311799
  REMARK    GeneRIF: Recurring homozygous ACTN2 variant (p.Arg506Gly) causes a
            recessive myopathy.
REFERENCE   3  (bases 1 to 4872)
  AUTHORS   Rodriguez Garcia,M., Schmeckpeper,J., Landim-Vieira,M.,
            Coscarella,I.L., Fang,X., Ma,W., Spran,P.A., Yuan,S., Qi,L.,
            Kahmini,A.R., Shoemaker,M.B., Atkinson,J.B., Kekenes-Huskey,P.M.,
            Irving,T.C., Chase,P.B., Knollmann,B.C. and Pinto,J.R.
  TITLE     Disruption of Z-Disc Function Promotes Mechanical Dysfunction in
            Human Myocardium: Evidence for a Dual Myofilament Modulatory Role
            by Alpha-Actinin 2
  JOURNAL   Int J Mol Sci 24 (19), 14572 (2023)
   PUBMED   37834023
  REMARK    GeneRIF: Disruption of Z-Disc Function Promotes Mechanical
            Dysfunction in Human Myocardium: Evidence for a Dual Myofilament
            Modulatory Role by Alpha-Actinin 2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4872)
  AUTHORS   Atang,A.E., Rebbeck,R.T., Thomas,D.D. and Avery,A.W.
  TITLE     Cardiomyopathy-associated variants alter the structure and function
            of the alpha-actinin-2 actin-binding domain
  JOURNAL   Biochem Biophys Res Commun 670, 12-18 (2023)
   PUBMED   37271035
  REMARK    GeneRIF: Cardiomyopathy-associated variants alter the structure and
            function of the alpha-actinin-2 actin-binding domain.
REFERENCE   5  (bases 1 to 4872)
  AUTHORS   Pavalko,F.M. and LaRoche,S.M.
  TITLE     Activation of human neutrophils induces an interaction between the
            integrin beta 2-subunit (CD18) and the actin binding protein
            alpha-actinin
  JOURNAL   J Immunol 151 (7), 3795-3807 (1993)
   PUBMED   8104223
REFERENCE   6  (bases 1 to 4872)
  AUTHORS   Cirino,A.L. and Ho,C.
  TITLE     Hypertrophic Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301725
REFERENCE   7  (bases 1 to 4872)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
REFERENCE   8  (bases 1 to 4872)
  AUTHORS   Beggs,A.H., Phillips,H.A., Kozman,H., Mulley,J.C., Wilton,S.D.,
            Kunkel,L.M. and Laing,N.G.
  TITLE     A (CA)n repeat polymorphism for the human skeletal muscle
            alpha-actinin gene ACTN2 and its localization on the linkage map of
            chromosome 1
  JOURNAL   Genomics 13 (4), 1314-1315 (1992)
   PUBMED   1505962
REFERENCE   9  (bases 1 to 4872)
  AUTHORS   Beggs,A.H., Byers,T.J., Knoll,J.H., Boyce,F.M., Bruns,G.A. and
            Kunkel,L.M.
  TITLE     Cloning and characterization of two human skeletal muscle
            alpha-actinin genes located on chromosomes 1 and 11
  JOURNAL   J Biol Chem 267 (13), 9281-9288 (1992)
   PUBMED   1339456
REFERENCE   10 (bases 1 to 4872)
  AUTHORS   Yuruker,B. and Niggli,V.
  TITLE     Alpha-actinin and vinculin in human neutrophils: reorganization
            during adhesion and relation to the actin network
  JOURNAL   J Cell Sci 101 (Pt 2), 403-414 (1992)
   PUBMED   1629252
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359921.13 and AL359185.25.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 27, 2020 this sequence version replaced NM_001103.3.
            
            Summary: Alpha actinins belong to the spectrin gene superfamily
            which represents a diverse group of cytoskeletal proteins,
            including the alpha and beta spectrins and dystrophins. Alpha
            actinin is an actin-binding protein with multiple roles in
            different cell types. In nonmuscle cells, the cytoskeletal isoform
            is found along microfilament bundles and adherens-type junctions,
            where it is involved in binding actin to the membrane. In contrast,
            skeletal, cardiac, and smooth muscle isoforms are localized to the
            Z-disc and analogous dense bodies, where they help anchor the
            myofibrillar actin filaments. This gene encodes a muscle-specific,
            alpha actinin isoform that is expressed in both skeletal and
            cardiac muscles. Several transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, May
            2013].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M86406.1, BC047901.2 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366578.6/ ENSP00000355537.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-301               AL359921.13        163599-163899
            302-416             AL359921.13        194958-195072
            417-536             AL359921.13        195994-196113
            537-623             AL359921.13        197205-197291
            624-711             AL359921.13        203033-203120
            712-790             AL359921.13        204778-204856
            791-872             AL359921.13        208333-208414
            873-958             AL359185.25        977-1062
            959-1051            AL359185.25        2464-2556
            1052-1282           AL359185.25        4644-4874
            1283-1430           AL359185.25        8238-8385
            1431-1581           AL359185.25        9968-10118
            1582-1690           AL359185.25        13009-13117
            1691-1831           AL359185.25        14466-14606
            1832-2014           AL359185.25        16812-16994
            2015-2149           AL359185.25        19289-19423
            2150-2329           AL359185.25        20361-20540
            2330-2476           AL359185.25        22828-22974
            2477-2542           AL359185.25        25066-25131
            2543-2701           AL359185.25        26357-26515
            2702-4872           AL359185.25        27803-29973
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q43"
     gene            1..4872
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="actinin alpha 2"
                     /db_xref="GeneID:88"
                     /db_xref="HGNC:HGNC:164"
                     /db_xref="MIM:102573"
     exon            1..301
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..2860
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     alpha-actinin skeletal muscle; F-actin cross-linking
                     protein"
                     /codon_start=1
                     /product="alpha-actinin-2 isoform 1"
                     /protein_id="NP_001094.1"
                     /db_xref="CCDS:CCDS1613.1"
                     /db_xref="GeneID:88"
                     /db_xref="HGNC:HGNC:164"
                     /db_xref="MIM:102573"
                     /translation="MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTF
                     TAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNK
                     ALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLW
                     CQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEI
                     AEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVN
                     QENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPK
                     VQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNE
                     IRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESD
                     LAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERME
                     KLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLP
                     EADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQS
                     LQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYE
                     HNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVET
                     QILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIM
                     TLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPP
                     DQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL"
     exon            302..416
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            417..536
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            537..623
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            624..711
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            712..790
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            791..872
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            873..958
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1051
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1282
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1430
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1581
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1690
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1831
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..2014
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2149
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2329
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2476
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2542
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2543..2701
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..4872
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3249..3254
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="hexamer: AATAAA"
     polyA_site      3274
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="major polyA site"
     regulatory      4759..4764
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="hexamer: AATAAA"
     polyA_site      4780
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
     regulatory      4846..4851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="hexamer: ATTAAA"
     polyA_site      4872
                     /gene="ACTN2"
                     /gene_synonym="CMD1AA; CMH23; CMYO8; CMYP8; MPD6; MYOCOZ"
                     /note="major polyA site"
ORIGIN      
        1 ggagctggtg cttcgcccga gacccagcgc ccaggcgtgt cgccccgaga ggagccgcgc
       61 gaaggtcacc ccgcgcccgc cgcccgccgc ccgccgcctc cgtgggtccg tttgccagtc
      121 agcccgtgcg tccgagcccc tcgcgccccg ccgcagcccc ggccaaccga gcgccatgaa
      181 ccagatagag cccggcgtgc agtacaacta cgtgtacgac gaggatgagt acatgatcca
      241 ggaggaggag tgggaccgcg acctgctcct ggacccagcc tgggagaagc agcagaggaa
      301 gaccttcact gcctggtgta actcccacct aaggaaagcc ggcacccaga ttgagaacat
      361 cgaggaagac ttcaggaatg gccttaagct catgctgctt ttggaagtca tctcagggga
      421 aaggctgccc aaacctgacc ggggaaaaat gcggttccac aaaattgcta atgtcaacaa
      481 agctttggat tacatagcca gcaaaggggt gaaactggtg tccattggcg ctgaagaaat
      541 tgttgatggc aacgtgaaaa tgaccctggg tatgatctgg accatcatcc ttcgctttgc
      601 tattcaggat atttcggttg aagaaacatc tgccaaagaa ggtctgctgc tttggtgtca
      661 gaggaaaact gctccttata gaaatgtgaa cattcagaac ttccatacta gctggaaaga
      721 tggccttgga ctctgtgccc tcatccaccg acaccggcct gacctcattg actactcaaa
      781 gcttaacaag gatgacccca taggaaatat taacctggcc atggaaatcg ctgagaagca
      841 cctggatatt cctaaaatgt tggatgctga agacatcgtg aacaccccta aacccgatga
      901 aagagccatc atgacgtacg tctcttgctt ctaccacgct tttgcgggcg cggagcaggc
      961 cgagacagcg gctaacagga tatgtaaggt tcttgctgtg aatcaagaga atgagaggct
     1021 gatggaagaa tatgagaggc tagcgagtga gcttttggaa tggattcgtc gcacgatccc
     1081 ctggctggag aaccggactc ccgagaagac catgcaagcc atgcagaaga agctggagga
     1141 cttccgggat taccgccgga agcacaagcc acccaaggtg caggagaaat gccagctgga
     1201 gatcaacttc aacacgctgc agaccaagct gcggatcagc aaccgtcctg ccttcatgcc
     1261 ctccgagggc aagatggtgt cggatattgc tggtgcctgg cagaggctgg agcaggctga
     1321 gaagggttac gaggagtggt tgctcaatga gattcggaga ctggagcgct tggaacacct
     1381 ggctgagaag ttcaggcaga aggcctcaac gcacgagact tgggcttatg gcaaagagca
     1441 gatcttgctg cagaaggatt acgagtcggc gtcgctgaca gaggtgcggg ctctgctgcg
     1501 gaagcacgag gcgttcgaga gcgacctggc agcgcaccag gaccgcgtgg agcagatcgc
     1561 agccatcgcg caggagctca atgaactgga ctatcacgac gctgtgaatg tcaatgatcg
     1621 gtgccagaaa atttgtgacc agtgggaccg actgggaacg cttactcaga agaggagaga
     1681 agccctagag agaatggaga aattgctaga aaccattgat cagcttcacc tggagtttgc
     1741 caagagggct gctcctttca acaattggat ggagggcgct atggaggatc tgcaagatat
     1801 gttcattgtc cacagcattg aggagatcca gagtctgatc actgcgcatg agcagttcaa
     1861 ggccacgctg cccgaggcgg acggagagcg gcagtccatc atggccatcc agaacgaggt
     1921 ggagaaggtg attcagagct acaacatcag aatcagctca agcaacccgt acagcactgt
     1981 caccatggat gagctccgga ccaagtggga caaggtgaag caactcgtgc ccatccgcga
     2041 tcaatccctg caggaggagc tggctcgcca gcatgctaac gagcgtctga ggcgccagtt
     2101 tgctgcccaa gccaatgcca ttgggccctg gatccagaac aagatggagg agattgcccg
     2161 gagctccatc cagatcacag gagccctgga agaccagatg aaccagctga agcagtatga
     2221 gcacaacatc atcaactata agaacaacat cgacaagctg gagggagacc atcagctcat
     2281 ccaggaggcc cttgtctttg acaacaagca cacgaactac acgatggagc acattcgtgt
     2341 tggatgggag ctgctgctga caaccatcgc cagaaccatc aatgaggtgg agactcagat
     2401 cctgacgaga gatgcgaagg gcatcaccca ggagcagatg aatgagttca gagcctcctt
     2461 caaccacttt gacaggagga agaatggcct gatggatcat gaggatttca gagcctgcct
     2521 gatttccatg ggttatgacc tgggtgaagc cgaatttgcc cgcattatga ccctggtaga
     2581 tcccaacggg caaggcaccg tcaccttcca atccttcatc gacttcatga ctagagagac
     2641 ggctgacacc gacactgccg agcaggtcat cgcctccttc cggatcctgg cttctgataa
     2701 gccatacatc ctggcggagg agctgcgtcg ggagctgccc ccggatcagg cccagtactg
     2761 catcaagagg atgcccgcct actcgggccc aggcagtgtg cctggtgcac tggattacgc
     2821 tgcgttctct tccgcactct acggggagag cgatctgtga tgctgagctt ctgtaatcac
     2881 tcatcccatc agaatgcaat aaaagcggaa gtcacagttt gtttcctgga aactttgaca
     2941 agctttatta agttgagaga gagagagggg aaaaaaaaaa gcctttcgta gttcagtaat
     3001 tgccagcaat ataacacggc taaaatgaag tttttacagt atatgacata gtgcgcttca
     3061 taaataggtt tatttctgag tttttagcaa aatgtaatga aatatcaggt tgatttcttt
     3121 gattaaacag aacaaattac ttgagtaata ggaaattagg aggatctagg gacagaagga
     3181 aagtgaaaaa tgtgaaaata caaaataccc aagatttaag accgggggga aaaaaccaca
     3241 aattggtaaa taaaggtttg ctatttgtaa aaaatttcat ttatctctaa tatgcttatg
     3301 tgattgcccc taggggagta tatttgggat tctaatgttt tattttcatg cttatccaaa
     3361 gattactatt gtatcttcaa atgaatttaa tattgtgaga tggaactgcc ggggattaaa
     3421 aagactaccc aaaagatttt tggcacttac aatttttaaa atagtttatg tcatctcttc
     3481 attatttagg gctggatggt caactcagtc agtgattttt tgatgcttct cttatcctcc
     3541 agaatagaga cctaaggaca cgtggaagtc agtttaattg ccagagagaa ggatgcaatc
     3601 actaggtaaa atgaggtttt taggattatt tattgattcc aggttcccat gctttttgtt
     3661 agagcttatt agtacaggtt ctcaagagat gaccacataa aagtgctctg tttataaata
     3721 agcaggtttc tgtagtactg actggttcat cacaaggcaa gtcagaaacc agtatccttc
     3781 tagctctcca gtcaggactt ccttatgcct ctagttttat gaccggttaa ggagaagcca
     3841 gagttagagt aggagaggac taattctcag cagcagtgga ggtgagttct ttcttttgcg
     3901 gaagctttac atatgttttg tgtagtagga ataactagat attttagcta gtgtgcggtg
     3961 tgtgttcacc cctgggattg gacagtgtat cctaacaagt cccatgtctg gttctgtgtc
     4021 taaaggcctg ctccatgaca caggatgcta catgcactcc tgctagcaca tcttgatctg
     4081 ttgaatgttc attctttctt tttgctcata ctgctgtagg ctataattcc cccctgtttt
     4141 tccatcttgt tgacagcttg tagagaataa agcaggaatt ctttttatat ctgagtcctt
     4201 aatgatcagg aaatggatta caaacaaaca aaaacagttg caaacagcaa ttaaagcatt
     4261 actagagaag cagtaacatg tctgttacct acagtctgtt tgctcagatt tttatagatt
     4321 tccaccaaca gctggaaatg taatatttcc attccaagct gtctgacagc ttgactcttc
     4381 ttcctacact gggccattta gaaccttcaa cctttatgaa tttcacttaa tttgcttcat
     4441 tattaagtaa ttggtagttt ccagattcct gaaatatttg aatccttgag tttaatgcca
     4501 aacacttttg gggtggttta gatgaactct gactctattt tatttcaagt aggtgctata
     4561 tcccggccat aaacccctat agaaaagcca gaaagttgtg cttcctttga ggctattagg
     4621 gggctctgtg gtgttttagg atgggtgtgg gtaactcatc tacctaaact tttaatttct
     4681 ttacaacatt cagcaagaga gggggtcagt agtctctatc tgctaataaa tgaagaaagg
     4741 aagtaaactc tgaaaaataa taaacatgtt tatactctca ttttaggtat tatttttatt
     4801 tctgaagctg gatgctcaaa aattaaaacg tttataagtt tctaaattaa acttaattta
     4861 taaaagaaaa aa
//
