LOCUS       NM_001009185            6535 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens acyl-CoA synthetase long chain family member 6
            (ACSL6), transcript variant 2, mRNA.
ACCESSION   NM_001009185
VERSION     NM_001009185.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Di,Y., Wang,Z., Xiao,J., Zhang,X., Ye,L., Wen,X., Qin,J., Lu,L.,
            Wang,X. and He,W.
  TITLE     ACSL6-activated IL-18R1-NF-kappaB promotes IL-18-mediated tumor
            immune evasion and tumor progression
  JOURNAL   Sci Adv 10 (38), eadp0719 (2024)
   PUBMED   39292786
  REMARK    GeneRIF: ACSL6-activated IL-18R1-NF-kappaB promotes IL-18-mediated
            tumor immune evasion and tumor progression.
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Xu,W., Tian,F., Tai,X., Song,G., Liu,Y., Fan,L., Weng,X., Yang,E.,
            Wang,M., Bornhauser,M., Zhang,C., Lock,R.B., Wong,J.W.H., Wang,J.,
            Jing,D. and Mi,J.Q.
  TITLE     ETV6::ACSL6 translocation-driven super-enhancer activation leads to
            eosinophilia in acute lymphoblastic leukemia through IL-3
            overexpression
  JOURNAL   Haematologica 109 (8), 2445-2458 (2024)
   PUBMED   38356460
  REMARK    GeneRIF: ETV6::ACSL6 translocation-driven super-enhancer activation
            leads to eosinophilia in acute lymphoblastic leukemia through IL-3
            overexpression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6535)
  AUTHORS   Kousathanas,A., Pairo-Castineira,E., Rawlik,K., Stuckey,A.,
            Odhams,C.A., Walker,S., Russell,C.D., Malinauskas,T., Wu,Y.,
            Millar,J., Shen,X., Elliott,K.S., Griffiths,F., Oosthuyzen,W.,
            Morrice,K., Keating,S., Wang,B., Rhodes,D., Klaric,L., Zechner,M.,
            Parkinson,N., Siddiq,A., Goddard,P., Donovan,S., Maslove,D.,
            Nichol,A., Semple,M.G., Zainy,T., Maleady-Crowe,F., Todd,L.,
            Salehi,S., Knight,J., Elgar,G., Chan,G., Arumugam,P., Patch,C.,
            Rendon,A., Bentley,D., Kingsley,C., Kosmicki,J.A., Horowitz,J.E.,
            Baras,A., Abecasis,G.R., Ferreira,M.A.R., Justice,A., Mirshahi,T.,
            Oetjens,M., Rader,D.J., Ritchie,M.D., Verma,A., Fowler,T.A.,
            Shankar-Hari,M., Summers,C., Hinds,C., Horby,P., Ling,L.,
            McAuley,D., Montgomery,H., Openshaw,P.J.M., Elliott,P., Walsh,T.,
            Tenesa,A., Fawkes,A., Murphy,L., Rowan,K., Ponting,C.P., Vitart,V.,
            Wilson,J.F., Yang,J., Bretherick,A.D., Scott,R.H., Hendry,S.C.,
            Moutsianas,L., Law,A., Caulfield,M.J. and Baillie,J.K.
  CONSRTM   GenOMICC investigators; 23andMe investigators; COVID-19 Human
            Genetics Initiative
  TITLE     Whole-genome sequencing reveals host factors underlying critical
            COVID-19
  JOURNAL   Nature 607 (7917), 97-103 (2022)
   PUBMED   35255492
REFERENCE   4  (bases 1 to 6535)
  AUTHORS   Kurotaki,A., Kuwata,H. and Hara,S.
  TITLE     Substrate Specificity of Human Long-Chain Acyl-CoA Synthetase ACSL6
            Variants
  JOURNAL   Biol Pharm Bull 44 (10), 1571-1575 (2021)
   PUBMED   34602568
  REMARK    GeneRIF: Substrate Specificity of Human Long-Chain Acyl-CoA
            Synthetase ACSL6 Variants.
REFERENCE   5  (bases 1 to 6535)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 6535)
  AUTHORS   Murati,A., Adelaide,J., Gelsi-Boyer,V., Etienne,A., Remy,V.,
            Fezoui,H., Sainty,D., Xerri,L., Vey,N., Olschwang,S., Birnbaum,D.,
            Chaffanet,M. and Mozziconacci,M.J.
  TITLE     t(5;12)(q23-31;p13) with ETV6-ACSL6 gene fusion in polycythemia
            vera
  JOURNAL   Leukemia 20 (6), 1175-1178 (2006)
   PUBMED   16572202
  REMARK    GeneRIF: The t(5;12)(q23-31;p13)translocation with ETV6-ACSL6
            genomic alteration rearrangement in polycythemia vera patients was
            reported.
REFERENCE   7  (bases 1 to 6535)
  AUTHORS   Mashek,D.G., Bornfeldt,K.E., Coleman,R.A., Berger,J.,
            Bernlohr,D.A., Black,P., DiRusso,C.C., Farber,S.A., Guo,W.,
            Hashimoto,N., Khodiyar,V., Kuypers,F.A., Maltais,L.J., Nebert,D.W.,
            Renieri,A., Schaffer,J.E., Stahl,A., Watkins,P.A., Vasiliou,V. and
            Yamamoto,T.T.
  TITLE     Revised nomenclature for the mammalian long-chain acyl-CoA
            synthetase gene family
  JOURNAL   J Lipid Res 45 (10), 1958-1961 (2004)
   PUBMED   15292367
REFERENCE   8  (bases 1 to 6535)
  AUTHORS   Malhotra,K.T., Malhotra,K., Lubin,B.H. and Kuypers,F.A.
  TITLE     Identification and molecular characterization of acyl-CoA
            synthetase in human erythrocytes and erythroid precursors
  JOURNAL   Biochem J 344 Pt 1 (Pt 1), 135-143 (1999)
   PUBMED   10548543
REFERENCE   9  (bases 1 to 6535)
  AUTHORS   Yagasaki,F., Jinnai,I., Yoshida,S., Yokoyama,Y., Matsuda,A.,
            Kusumoto,S., Kobayashi,H., Terasaki,H., Ohyashiki,K., Asou,N.,
            Murohashi,I., Bessho,M. and Hirashima,K.
  TITLE     Fusion of TEL/ETV6 to a novel ACS2 in myelodysplastic syndrome and
            acute myelogenous leukemia with t(5;12)(q31;p13)
  JOURNAL   Genes Chromosomes Cancer 26 (3), 192-202 (1999)
   PUBMED   10502316
REFERENCE   10 (bases 1 to 6535)
  AUTHORS   Lehner,R. and Kuksis,A.
  TITLE     Biosynthesis of triacylglycerols
  JOURNAL   Prog Lipid Res 35 (2), 169-201 (1996)
   PUBMED   8944226
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA200154.1, AB020644.1,
            AC034228.6 and BM992189.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 31, 2019 this sequence version replaced NM_001009185.2.
            
            Summary: The protein encoded by this gene catalyzes the formation
            of acyl-CoA from fatty acids, ATP, and CoA, using magnesium as a
            cofactor. The encoded protein plays a major role in fatty acid
            metabolism in the brain. Translocations with the ETV6 gene are
            causes of myelodysplastic syndrome with basophilia, acute
            myelogenous leukemia with eosinophilia, and acute eosinophilic
            leukemia. Several transcript variants encoding different isoforms
            have been found for this gene.[provided by RefSeq, Apr 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB020644.1, SRR1660805.27911.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: locus in the vicinity of
                                      disease-associated variant(s)
            MANE Ensembl match     :: ENST00000651883.2/ ENSP00000499063.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                DA200154.1         15-50
            37-2909             AB020644.1         1-2873
            2910-6518           AC034228.6         125037-128645
            6519-6535           BM992189.1         16-32               c
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.1"
     gene            1..6535
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="acyl-CoA synthetase long chain family member 6"
                     /db_xref="GeneID:23305"
                     /db_xref="HGNC:HGNC:16496"
                     /db_xref="MIM:604443"
     exon            1..154
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     CDS             106..2274
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /EC_number="6.2.1.3"
                     /EC_number="6.2.1.15"
                     /note="isoform b is encoded by transcript variant 2;
                     fatty-acid-Coenzyme A ligase, long-chain 6;
                     long-chain-fatty-acid--CoA ligase 6; long fatty acyl-CoA
                     synthetase 2; arachidonate--CoA ligase"
                     /codon_start=1
                     /product="long-chain-fatty-acid--CoA ligase 6 isoform b"
                     /protein_id="NP_001009185.1"
                     /db_xref="CCDS:CCDS34229.1"
                     /db_xref="GeneID:23305"
                     /db_xref="HGNC:HGNC:16496"
                     /db_xref="MIM:604443"
                     /translation="MLTFFLVSGGSLWLFVEFVLSLLEKMQTQEILRILRLPELGDLG
                     QFFRSLSATTLVSMGALAAILAYWFTHRPKALQPPCNLLMQSEEVEDSGGARRSVIGS
                     GPQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGS
                     GLLQHNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTA
                     DISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAV
                     EDCGQENHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEKVIF
                     PRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRLLSDDMKALCPTIFPVVP
                     RLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASLG
                     GCVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAP
                     LPCNHIKLVDVEELNYWACKGEGEICVRGPNVFKGYLKDPDRTKEALDSDGWLHTGDI
                     GKWLPAGTLKIIDRKKHIFKLAQGEYVAPEKIENIYIRSQPVAQIYVHGDSLKAFLVG
                     IVVPDPEVMPSWAQKRGIEGTYADLCTNKDLKKAILEDMVRLGKESGLHSFEQVKAIH
                     IHSDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYSISM"
     exon            155..375
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            376..490
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            491..555
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            556..657
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            658..757
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            758..936
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            937..969
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1021
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1095
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1173
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1308
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1443
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1539
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1612
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1701
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1818
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1962
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2064
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2136
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..6535
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2583..2588
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="hexamer: ATTAAA"
     regulatory      2589..2594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="hexamer: CATAAA"
     polyA_site      2603
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="major polyA site"
     polyA_site      2606
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="major polyA site"
     regulatory      6511..6516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="hexamer: AATAAA"
     polyA_site      6535
                     /gene="ACSL6"
                     /gene_synonym="ACS2; FACL6; LACS 6; LACS2; LACS5"
                     /note="major polyA site"
ORIGIN      
        1 agcggcggcg gcggcgcggc tactgctggg gctgctgggc tgcggggctg cggggctgcg
       61 ggtcggggag gccgggccgg gccgggccgg gccccgctga ccgccatgct gaccttcttc
      121 ctcgtgtcgg ggggctccct ctggctattc gtagagtttg tcctctcact tctggagaag
      181 atgcagacac aggagatcct gaggatactg cgactgcctg agctaggtga cttgggacag
      241 tttttccgca gcctctcggc caccaccctc gtgagtatgg gtgccctggc tgccatcctt
      301 gcctactggt tcactcaccg gccaaaggcc ttgcagccgc catgcaacct cctgatgcag
      361 tcagaagaag tagaggacag tggcggggca cggcgatctg tgattgggtc tggccctcag
      421 ctacttaccc actactatga tgatgcccgg accatgtacc aggtgttccg ccgtgggctt
      481 agcatctcag ggaatgggcc ctgtcttggt ttcaggaagc ctaagcagcc ttaccagtgg
      541 ctgtcctacc aggaggtggc cgacagggct gaatttctgg ggtccggact tctccagcac
      601 aattgtaaag catgcactga tcagtttatt ggtgtttttg cacaaaatcg gccagagtgg
      661 atcattgtgg agctggcctg ctacacatat tccatggtgg tggtcccgct ctatgacacc
      721 ctgggccctg gggctatccg ctacatcatc aatacagcgg acatcagcac cgtgattgtg
      781 gacaaacctc agaaggctgt gcttctgcta gagcatgtgg agaggaagga gactccaggc
      841 ctcaagctga tcatcctcat ggacccattc gaagaagccc tgaaagagag agggcagaag
      901 tgcggggtgg tcattaagtc catgcaggcc gtggaggact gtggccaaga gaatcaccag
      961 gctcctgtgc ccccgcagcc tgatgacctc tccattgtgt gtttcacaag cggcacgaca
     1021 gggaacccaa aaggtgcgat gctcacccat gggaacgtgg tggctgattt ctcaggcttt
     1081 ctgaaagtga cagagaaagt gatctttccg agacaggacg atgtgctcat ctccttcctg
     1141 cctctggctc acatgtttga gagagtaatc cagtctgtcg tctattgcca cggagggcgt
     1201 gttggcttct tccagggaga tatccgcctt ctctcagatg acatgaaggc tctatgcccc
     1261 accatcttcc ctgtggtccc acgactgctg aaccggatgt acgacaagat cttcagccag
     1321 gcaaacacac cattaaagcg ctggctcctg gagtttgcag caaagcgtaa gcaagccgag
     1381 gtccggagtg gaatcatcag gaatgatagt atctgggatg aactcttctt taataagatt
     1441 caggccagtc ttggtgggtg tgtgcggatg attgttactg gagcagcccc agcatcacca
     1501 acagttctgg gatttctccg ggcagctcta gggtgccagg tttatgaagg ttatggccaa
     1561 actgagtgca cagctggatg taccttcacc actcctggcg actggacctc agggcacgta
     1621 ggggcgccac ttccctgcaa tcatatcaag ctcgttgatg ttgaggaact gaactactgg
     1681 gcctgcaaag gagagggaga gatatgtgtg agaggaccaa atgtgttcaa aggctacttg
     1741 aaagatccag acaggacgaa ggaggccctg gacagcgatg gctggcttca cactggagac
     1801 atcggaaaat ggctgccggc aggaactctt aaaattattg atcggaaaaa gcatatattt
     1861 aaacttgctc agggagaata tgttgcaccc gagaagattg agaacatcta catccggagc
     1921 caacctgtgg cgcaaatcta tgtccatggg gacagcttaa aggccttttt ggtaggcatt
     1981 gttgtgcctg accctgaagt tatgccctcc tgggcccaga agagaggaat tgaaggaaca
     2041 tatgcagatc tctgcacaaa taaggatctg aagaaagcca ttttggaaga tatggtgagg
     2101 ttaggaaaag aaagtggact ccattctttt gagcaggtta aagccattca catccattct
     2161 gacatgttct cagttcaaaa tggcttgctg acaccaacac taaaagctaa gagacctgag
     2221 ctgagagagt acttcaaaaa acaaatagaa gagctttact caatctccat gtgaagttca
     2281 aggaaagttc ttctcagtgt aatgaactgt ctagcaatat tatagttatt cttgaaagta
     2341 atgagtcaaa atgacacagc tgaaaatgaa taagcatctg attttatgac tgagcctttt
     2401 cctgtcccaa gaggtcttta acaatatttt ctctatcatc aatgagtata ttttattttt
     2461 attataaaaa tgatattgtg gtggactgct aaaaatatca caaatggcaa tgtaaaaatc
     2521 aagacatttt ctcaagaact gtgtaccact aaaagtaata tattgtcaat gttcacagaa
     2581 ctattaaaca taaaggaaaa acataagtga tatattctac ttaattattt gtgaatcagt
     2641 aaccagatgc agcaaatatc taggcaatgt ggactacctc attcagtaac tgattgtcaa
     2701 aatcacaatt aaatcagact tcaaaaatta aagctaggtg tatagaatca tgctaaaaga
     2761 aaacatgata actcatagtc tacgtaactt cagagtcttt aaacatgaca atccacattg
     2821 tcatatgtga aaattttctc tctgattttt actttcattc atgaaaaatg aaaattcaga
     2881 aattcttttt ttcctttttg ttttgagaca gggtctgctc tgtcacctag gctggagtgc
     2941 agtggcttaa tcatggctca ttgcagtctc catctcctgg gctcgagtga tcctcctgtc
     3001 tcacctccca agtagctgag actacagtac aggcacatgc caccacacct ggctaataga
     3061 aatttttttt ttagagattt tgctcaggct ggtctcaaac tcctgagctc aagggatcct
     3121 cccgccttgg cctccctaag tgctaggatt acaggcatga gccattgttc ccagccaaat
     3181 tcagatatta ttaaaacaca tgtcatattt atatagtaac ttacaaagac ctttcaatac
     3241 attttctcat ttattaagct cattaaagta ttcaggaact acctagaaaa aatataatgt
     3301 aaaactattc aaggatagtg tgtgtatgtt catggacttc ttattataat gaattctaaa
     3361 agacatctgt tgactctaca atgaatggat ccttgaggaa tacttgggag aagaaactca
     3421 gagttatttc tcagggtagg cagcaattaa tgtacctaca ttccttgctg gggtcttcta
     3481 gtcttccatt cccaatgtgc ccatgctatg cctggaaacc ctatatggtt gtaattctga
     3541 acaatttcac tttttttcca gtaagaatat caaggcagaa ggtgggaagg aggggacatt
     3601 atttccaggg aaaatagttt ttcaacaata taactttgat aaacctcttt aaaatgcccc
     3661 aagaaaactt tttaagtcca tagacaaaga aatactgcct aatggcataa ttacattcct
     3721 aaaatcttta agcgtgccga agtttaacca ctaaaacctc ctttcttgca ttatgtattt
     3781 agatgcaccc tgtattgggg tgtcaacaat ttcttataat taaaggccag ataccatgga
     3841 cagcaattaa gttccaagct atagattgtg cctctgaaaa aggcatggac cccaggaacg
     3901 tgtttttctt ctgtagagac aagactctaa aagcatatca acaatccata tgcaattcat
     3961 gtgttaattt aaaatgtatg tgctcagtgt ttgtagtcta gaagctcctt tcccttggag
     4021 gaatgccaag cagtttgcaa aaataaatgc tgttagttaa aaaccacata atcacatggg
     4081 cctactgaat aaatatgcat cagtgattat atacttatat ttcagtcttg tcaaaagtga
     4141 atcactgttt catttgatgt atttacccag tctttttatc cagtttttct tgggcatatt
     4201 ctctctgaag acccactgtt gcacttctaa atttgacagt taagaaatga gctagttcta
     4261 tacacactga tttttaaagg cgtttctgaa taaactaata cttaaaatgt ccaaagtcac
     4321 atctgtacag cattagattt ttatatttaa tatatatttg actaattaaa agtgaaagtt
     4381 gttacctgaa ctggatattc atactatttt aagggcaagt tgcttacatt tcaataacaa
     4441 caaaaaaaga atctgtttcc cattgtcctc ctactcaact aaaattcata gttggcttta
     4501 agcccaaaag aattttgaac aatgtgacag aaacaagtaa tgtaaaactt attttgtttt
     4561 atttatactt tataatagtt agatataaca gattatggac aacttaatat ttcttctttt
     4621 tggctgggcg cggtggctca tgcctgtaat cccagcactt tgggaggccg aggcgggcag
     4681 atcacgaagt caggagatcg agaccatcct ggctaacaca gtgaaacccc gtctctacta
     4741 aaaaaataca aaaaattagc cgggcgttgt ggcgggcgcc tgtagtccca gctactcggg
     4801 aggctgaggc aggagaatgg catgaacctg ggaggcggag cttgcagtga gccgagatcc
     4861 cgccactgta ctccagcctg ggcaacagaa cgagactccg tctcaaaaag aaagaaaaaa
     4921 aaaaaacaaa aacaaggttt cttctttttg ccacttaaaa tatcttatat ttctagattt
     4981 ccaggtctat gtggaaatca tcacacttgg gccaataaat gggttagttc agatgtgctg
     5041 agaaaagtaa atagtgtacc ctaaatatct tgcatgtaca tttatctatt tctctgttta
     5101 atgccactta attttgtaag aaatgagaaa tgaaacccta tgtatcagtg gaaaaatcct
     5161 tcttgcttca gatttcccaa ctttattctg gaggagaaaa aaatctgtat ttctcattaa
     5221 cagagataga aaaaggtgat gtacttacga tggtcacatt catattgggc attggatcta
     5281 tttaagccta gaagtataac agataatcag ttccttaagt gtaaatatgt tcttaaatgt
     5341 ttggcctttt tatctgttat tcaaatgtat tagtaattct catattttgc aaacagatcc
     5401 ttttgtgtat attataaaat atataccaaa tctgtttttc cttttgctgt ggaagatgtc
     5461 tttatataat attgagactt cccttgcaaa tgagagacgg gactctggtc aggtatagtg
     5521 tgtaggccta gccccatgta ttaggaactc ataaactcag aaatccaagg attaaacatt
     5581 ttatgtctcc ttcatttatg caagtcatgc tttcattcta catgtagtta aaactacaag
     5641 tatgagaaaa aatagaactt tccattggaa ttttttccaa tcaggtaatg ttttagcaac
     5701 ttgaaatcag cacttaattt gattgtatta cctatcaaag aaccaataac ctttcctttt
     5761 tctgcagtaa agaaaatgaa atatccttta ttttccaaat gtatgaagca caaatgcaat
     5821 atagttattt aaaatataaa atactttaaa atgaatgtga agaatgggaa aatatatttt
     5881 tgcatgaaaa cagactactt acaaatgtgc ttaatatctc aggaaaaaaa gaatgaataa
     5941 ccgtatataa aattggtaaa ctataaattg gctgtatttc cttgccggtt ttaattttaa
     6001 ggaagcattt acatgaaaag aaaaaaggat cctagtagtc tgaatttcct ctcaagtatg
     6061 ctagaaaata ctttctatga atttacaatt aactgcaata agaagtgggt tagcattcaa
     6121 gtaaggtcaa taccccaaat gagaaactct tcagttagaa cacattagtg gttgtaacac
     6181 agaggcagta gataaatatt tgaaatgtga gcatttctaa acttggtacc aaatggaaag
     6241 ggtttcatga ggtgacagtg gcttttaaac attcatttga ccgattattc taattttcct
     6301 tgttaaatca ttgcaacagt tgcatggggg agaatggata ttttttgaag aattgcttat
     6361 tccactttca atttttaaaa atcgagggga ttaaaatatt actttttaaa tatgcataaa
     6421 agtatcttaa ctgtaattac attttcagtt gctttgttat agtagcatgc atattaaata
     6481 taaaatgcat ttatgaaaat ttttgtaaaa aataaaagat ttttcatttc tcaaa
//
