LOCUS       NM_004457               5577 bp    mRNA    linear   PRI 13-NOV-2024
DEFINITION  Homo sapiens acyl-CoA synthetase long chain family member 3
            (ACSL3), transcript variant 1, mRNA.
ACCESSION   NM_004457
VERSION     NM_004457.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Han,S., Guo,J., Kong,C., Li,J., Lin,F., Zhu,J., Wang,T., Chen,Q.,
            Liu,Y., Hu,H., Qiu,T., Cheng,F. and Zhou,J.
  TITLE     ANKRD1 aggravates renal ischaemia-reperfusion injury via promoting
            TRIM25-mediated ubiquitination of ACSL3
  JOURNAL   Clin Transl Med 14 (9), e70024 (2024)
   PUBMED   39285846
  REMARK    GeneRIF: ANKRD1 aggravates renal ischaemia-reperfusion injury via
            promoting TRIM25-mediated ubiquitination of ACSL3.
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Yang,L. and Yuan,Z.H.
  TITLE     [Research advances on the role of ACSL3 in the atherosclerosis]
  JOURNAL   Sheng Li Xue Bao 75 (4), 587-594 (2023)
   PUBMED   37583046
  REMARK    GeneRIF: [Research advances on the role of ACSL3 in the
            atherosclerosis].
REFERENCE   3  (bases 1 to 5577)
  AUTHORS   Ma,M., Kong,P., Huang,Y., Wang,J., Liu,X., Hu,Y., Chen,X., Du,C.
            and Yang,H.
  TITLE     Activation of MAT2A-ACSL3 pathway protects cells from ferroptosis
            in gastric cancer
  JOURNAL   Free Radic Biol Med 181, 288-299 (2022)
   PUBMED   35182729
  REMARK    GeneRIF: Activation of MAT2A-ACSL3 pathway protects cells from
            ferroptosis in gastric cancer.
REFERENCE   4  (bases 1 to 5577)
  AUTHORS   Fernandez,L.P., Merino,M., Colmenarejo,G., Moreno-Rubio,J.,
            Sanchez-Martinez,R., Quijada-Freire,A., Gomez de Cedron,M.,
            Reglero,G., Casado,E., Sereno,M. and Ramirez de Molina,A.
  TITLE     Metabolic enzyme ACSL3 is a prognostic biomarker and correlates
            with anticancer effectiveness of statins in non-small cell lung
            cancer
  JOURNAL   Mol Oncol 14 (12), 3135-3152 (2020)
   PUBMED   33030783
  REMARK    GeneRIF: Metabolic enzyme ACSL3 is a prognostic biomarker and
            correlates with anticancer effectiveness of statins in non-small
            cell lung cancer.
REFERENCE   5  (bases 1 to 5577)
  AUTHORS   Ndiaye,H., Liu,J.Y., Hall,A., Minogue,S., Morgan,M.Y. and
            Waugh,M.G.
  TITLE     Immunohistochemical staining reveals differential expression of
            ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic
            gastrointestinal metastases
  JOURNAL   Biosci Rep 40 (4) (2020)
   PUBMED   32286604
  REMARK    GeneRIF: Immunohistochemical staining reveals differential
            expression of ACSL3 and ACSL4 in hepatocellular carcinoma and
            hepatic gastrointestinal metastases.
REFERENCE   6  (bases 1 to 5577)
  AUTHORS   Fujino,T., Kang,M.J., Suzuki,H., Iijima,H. and Yamamoto,T.
  TITLE     Molecular characterization and expression of rat acyl-CoA
            synthetase 3
  JOURNAL   J Biol Chem 271 (28), 16748-16752 (1996)
   PUBMED   8663269
REFERENCE   7  (bases 1 to 5577)
  AUTHORS   Rose,C.S., King,A.A., Summers,D., Palmer,R., Yang,S., Wilkie,A.O.,
            Reardon,W., Malcolm,S. and Winter,R.M.
  TITLE     Localization of the genetic locus for Saethre-Chotzen syndrome to a
            6 cM region of chromosome 7 using four cases with apparently
            balanced translocations at 7p21.2
  JOURNAL   Hum Mol Genet 3 (8), 1405-1408 (1994)
   PUBMED   7987323
REFERENCE   8  (bases 1 to 5577)
  AUTHORS   Brueton,L.A., van Herwerden,L., Chotai,K.A. and Winter,R.M.
  TITLE     The mapping of a gene for craniosynostosis: evidence for linkage of
            the Saethre-Chotzen syndrome to distal chromosome 7p
  JOURNAL   J Med Genet 29 (10), 681-685 (1992)
   PUBMED   1433226
REFERENCE   9  (bases 1 to 5577)
  AUTHORS   Bakken,A.M., Farstad,M. and Holmsen,H.
  TITLE     Identity between palmitoyl-CoA synthetase and arachidonoyl-CoA
            synthetase in human platelet?
  JOURNAL   Biochem J 274 (Pt 1) (Pt 1), 145-152 (1991)
   PUBMED   1848073
REFERENCE   10 (bases 1 to 5577)
  AUTHORS   Bronfman,M., Inestrosa,N.C., Nervi,F.O. and Leighton,F.
  TITLE     Acyl-CoA synthetase and the peroxisomal enzymes of beta-oxidation
            in human liver. Quantitative analysis of their subcellular
            localization
  JOURNAL   Biochem J 224 (3), 709-720 (1984)
   PUBMED   6240978
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC041692.1 and AC013476.9.
            
            On Nov 22, 2018 this sequence version replaced NM_004457.4.
            
            Summary: The protein encoded by this gene is an isozyme of the
            long-chain fatty-acid-coenzyme A ligase family. Although differing
            in substrate specificity, subcellular localization, and tissue
            distribution, all isozymes of this family convert free long-chain
            fatty acids into fatty acyl-CoA esters, and thereby play a key role
            in lipid biosynthesis and fatty acid degradation. This isozyme is
            highly expressed in brain, and preferentially utilizes myristate,
            arachidonate, and eicosapentaenoate as substrates. The amino acid
            sequence of this isozyme is 92% identical to that of rat homolog.
            Two transcript variants encoding the same protein have been found
            for this gene. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.58269.1,
                                           SRR1660803.141400.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357430.8/ ENSP00000350012.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2048              BC041692.1         15-2062
            2049-2049           AC013476.9         52113-52113
            2050-3165           BC041692.1         2064-3179
            3166-5577           AC013476.9         63641-66052
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q36.1"
     gene            1..5577
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="acyl-CoA synthetase long chain family member 3"
                     /db_xref="GeneID:2181"
                     /db_xref="HGNC:HGNC:3570"
                     /db_xref="MIM:602371"
     exon            1..223
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            224..282
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            283..389
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            390..807
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     CDS             430..2592
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /EC_number="6.2.1.3"
                     /EC_number="6.2.1.2"
                     /EC_number="6.2.1.15"
                     /note="lignoceroyl-CoA synthase; fatty-acid-Coenzyme A
                     ligase, long-chain 3; long-chain-fatty-acid--CoA ligase 3;
                     long-chain acyl-CoA synthetase 3; arachidonate--CoA
                     ligase; medium-chain acyl-CoA ligase Acsl3"
                     /codon_start=1
                     /product="fatty acid CoA ligase Acsl3"
                     /protein_id="NP_004448.2"
                     /db_xref="CCDS:CCDS2455.1"
                     /db_xref="GeneID:2181"
                     /db_xref="HGNC:HGNC:3570"
                     /db_xref="MIM:602371"
                     /translation="MNNHVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYFF
                     SESRQEKSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYPGCDTLDKVFTYAKNKFKNK
                     RLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQMLGQKPKT
                     NIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQ
                     TKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKP
                     LPSDIAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVL
                     ELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYK
                     NVMNKVSEMSSFQRNLFILAYNYKMEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLC
                     GGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVWDYNTGRVGAPLVCCEIKL
                     KNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWLCTGDIG
                     EFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGF
                     VVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRL
                     SPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGRK"
     exon            808..985
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1095
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1234
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1385
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1509
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1581
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1721
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1894
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..1969
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2161
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2276
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2434
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..5577
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2741..2746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="hexamer: AGTAAA"
     polyA_site      2762
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="major polyA site"
     regulatory      3134..3139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="hexamer: AATATA"
     polyA_site      3157
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="major polyA site"
     regulatory      5540..5545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="hexamer: AATAAA"
     polyA_site      5562
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="major polyA site"
     regulatory      5572..5577
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
                     /note="hexamer: ATTAAA"
     polyA_site      5577
                     /gene="ACSL3"
                     /gene_synonym="ACS3; FACL3; LACS 3; LACS3; PRO2194"
ORIGIN      
        1 agacgctgct gtggctgcgc cgggctgcga cactgcagtt gtctacgcgg ccggggccgg
       61 gacgaggagg cgttggacgg ggtcgcatac gttcgtcccc tcgcattgcg gccccgacag
      121 ctgcgccagg atccccgggc ggcggcgcgg ggcgtgaacg ctctggggct cagccaggcc
      181 tgcgcgggcc cgaggccgga ggaacccgga ctccggcgta gcggttttga cacaagggcg
      241 catatcttca aagcacctag tacctcctac cattgtcaac tgatacagaa ttcgttgttg
      301 ggaaggactg gggaaacagc tgtaacattt gccaccctca gaagctgctg gtcctgtgtc
      361 acaccacctt agcctcttga tcgaggaaga ttctcgctga agtctgttaa ttctactttt
      421 tgagtactta tgaataacca cgtgtcttca aaaccatcta ccatgaagct aaaacatacc
      481 atcaacccta ttcttttata ttttatacat tttctaatat cactttatac tattttaaca
      541 tacattccgt tttatttttt ctccgagtca agacaagaaa aatcaaaccg aattaaagca
      601 aagcctgtaa attcaaaacc tgattctgca tacagatctg ttaatagttt ggatggtttg
      661 gcttcagtat tataccctgg atgtgatact ttagataaag tttttacata tgcaaaaaac
      721 aaatttaaga acaaaagact cttgggaaca cgtgaagttt taaatgagga agatgaagta
      781 caaccaaatg gaaaaatttt taaaaaggtt attcttggac agtataattg gctttcctat
      841 gaagatgtct ttgttcgagc ctttaatttt ggaaatggat tacagatgtt gggtcagaaa
      901 ccaaagacca acatcgccat cttctgtgag accagggccg agtggatgat agctgcacag
      961 gcgtgtttta tgtataattt tcagcttgtt acattatatg ccactctagg aggtccagcc
     1021 attgttcatg cattaaatga aacagaggtg accaacatca ttactagtaa agaactctta
     1081 caaacaaagt tgaaggatat agtttctttg gtcccacgcc tgcggcacat catcactgtt
     1141 gatggaaagc caccgacctg gtccgagttc cccaagggca tcattgtgca taccatggct
     1201 gcagtggagg ccctgggagc caaggccagc atggaaaacc aacctcatag caaaccattg
     1261 ccctcagata ttgcagtaat catgtacaca agtggatcca caggacttcc aaagggagtc
     1321 atgatctcac atagtaacat tattgctggt ataactggga tggcagaaag gattccagaa
     1381 ctaggagagg aagatgtcta cattggatat ttgcctctgg cccatgttct agaattaagt
     1441 gctgagcttg tctgtctttc tcacggatgc cgcattggtt actcttcacc acagacttta
     1501 gcagatcagt cttcaaaaat taaaaaagga agcaaagggg atacatccat gttgaaacca
     1561 acactgatgg cagcagttcc ggaaatcatg gatcggatct acaaaaatgt catgaataaa
     1621 gtcagtgaaa tgagtagttt tcaacgtaat ctgtttattc tggcctataa ttacaaaatg
     1681 gaacagattt caaaaggacg taatactcca ctgtgcgaca gctttgtttt ccggaaagtt
     1741 cgaagcttgc tagggggaaa tattcgtctc ctgttgtgtg gtggcgctcc actttctgca
     1801 accacgcagc gattcatgaa catctgtttc tgctgtcctg ttggtcaggg atacgggctc
     1861 actgaatctg ctggggctgg aacaatttcc gaagtgtggg actacaatac tggcagagtg
     1921 ggagcaccat tagtttgctg tgaaatcaaa ttaaaaaact gggaggaagg tggatacttt
     1981 aatactgata agccacaccc caggggtgaa attcttattg ggggccaaag tgtgacaatg
     2041 gggtactaca aaaatgaagc aaaaacaaaa gctgatttct ttgaagatga aaatggacaa
     2101 aggtggctct gtactgggga tattggagag tttgaacccg atggatgctt aaagattatt
     2161 gatcgtaaaa aggaccttgt aaaactacag gcaggggaat atgtttctct tgggaaagta
     2221 gaggcagctt tgaagaatct tccactagta gataacattt gtgcatatgc aaacagttat
     2281 cattcttatg tcattggatt tgttgtgcca aatcaaaagg aactaactga actagctcga
     2341 aagaaaggac ttaaagggac ttgggaggag ctgtgtaaca gttgtgaaat ggaaaatgag
     2401 gtacttaaag tgctttccga agctgctatt tcagcaagtc tggaaaagtt tgaaattcca
     2461 gtaaaaattc gtttgagtcc tgaaccgtgg acccctgaaa ctggtctggt gacagatgcc
     2521 ttcaagctga aacgcaaaga gcttaaaaca cattaccagg cggacattga gcgaatgtat
     2581 ggaagaaaat aattattctc ttctggcatc agtttgctac agtgagctca gatcaaatag
     2641 gaaaatactt gaaatgcatg tctcaagctg caaggcaaac tccattcctc atattaaact
     2701 attacttctc atgacgtcac catttttaac tgacaggatt agtaaaacat taagacagca
     2761 aacttgtgtc tgtctcttct ttcattttcc ccgccaccaa cttactttac cacctatgac
     2821 tgtacttgtc agtatgagaa tttttctgaa tcatattggg gaagcagtga ttttaaaacc
     2881 tcaagttttt aaacatgatt tatatgttct gtataatgtt cagtttgtaa ctttttaaaa
     2941 gtttggatgt atagagggat aaataggaaa tataagaatt ggttatttgg gggctttttt
     3001 acttactgta tttaaaaata caagggtatt gatatgaaat tatgtaaatt tcaaatgctt
     3061 atgaatcaaa tcattgttga acaaaagatt tgttgctgtg taattattgt cttgtatgca
     3121 tttgagagaa ataaatatac ccatacttat gttttaagaa gttgagatct tgtgaatata
     3181 tgcctgtcag tgtcttcttt atatatttat tttttattag aaaaaatgaa gtttggttgg
     3241 tgatgcatga aacaaaatag caagagaggg ttatagttta atagtaaggg agataacaca
     3301 gcatgtgtag caccagttga taattggtct ctagtagctt actgtcaaaa tgttcaatga
     3361 agtcttctgt tcatctgttg aaactaggaa aatacccaaa cttaaatgga agaattctga
     3421 aagagaggat agaatttaaa gaacaagagt atataaagtt attctttgaa tatttcgttg
     3481 actatatgta cattgagtta tctatatttg taaacaaatt agtcatggaa aattattcta
     3541 tctcaaagtc tccttttagt ctagataatc attatttcat tttaaaatta gtgtttttcc
     3601 tagtttgcac tgatgcgtgt atggatgtgt gtgagtcagt ggtagcttat ttaaaaagca
     3661 ccttatcctt tctcccataa cctttgtaca ctaaaaaatg aaagaattta gaatgtattt
     3721 gatgatagca ttctcactaa gacacatgag aatttaactt tataaccgcg tgagttaaga
     3781 tttaattcat aggttttgat gtcattgttg aagttatttg taattcagaa accttgcttg
     3841 tgtgatacat agtctcttca tttattactg cttgtctgtt gttatatctg gattatcaaa
     3901 agcaatagtg caccaattaa gatgtgctca aatcaggact taaatcatag gcaccacatt
     3961 tttcatgtca gactagttac tttgttgatt ctcagttact gtaggcatca aaaggcaaaa
     4021 atcaaaaaaa aaaaaaacaa aaacaaaaaa aaagatgaac ctaggtctgt gtaaagtaag
     4081 gggagtgtta ggagcagcca ggactgtgta gtgtgtgttt ggttgcatca caaacatcgt
     4141 atgtggagac attgcaatac agtgtttttt gttttcaact tttcttgtat tgtatatttg
     4201 tattatgttt tgaatgcttt tctcttttca taattaaata ttaatgtttg ggataactgc
     4261 caagaagaag taaaaatatt gaatggaact tctatatgag gatgctgtga tctaaaaatt
     4321 aaatctcagt gggcggagag aatttgtttc tgatgccttg tctttagaat aattgtttgc
     4381 tttcagatga ttaaaaaaag gagattgtat ctaggaaaaa agtgtgaatg cttcaaagga
     4441 gagatactga taattgtgac ttgaaatagt gtcagatgaa ttatgtagct gatcactgat
     4501 ggaaccatct aatgaggcag gcttaaactc tatttagttt cgtttgtttt ctcatatgaa
     4561 gtttataatt cctttcagtc ctaaaatgtc agatgccagg tgtcaaatgc cacagtagaa
     4621 agtgaaatta agtagagtcc ccttttaaac aaatttgtct gtaagcaaaa ctatctttag
     4681 tgacttaaaa aataatatcc tctaaaatta ccctttcaag aggaaaaaac tgttcaatga
     4741 aaaaaccact caattatgac tcatataaga aatactggtt taaccaggag atatttgggg
     4801 agcaaaattt taaggaattt caaattgcta aatctaatac aaatatctga caataagata
     4861 cagatctaaa agaatggttc ttaagaacta ttgtttactg ggaataaact aagctctatg
     4921 aagaattcgt aagcattaca tgttccagta agtttagcaa cttagagcca agactggtgg
     4981 ccacctccat ttatttcccc actgctcctc cactgacatc cttcttagca gaaacttcat
     5041 gaaaaagttt ttgctgacac agaacaaacc tgaaagtagt atctactttc taaatactac
     5101 tttgcttttc agtagtggat ttgatattta taatgttctc taaagcttgc aactttttca
     5161 gcaacgttta aaaatagatt aacctggaat aacttacttg tttgctgcta aaatactcaa
     5221 gattttgcca tttttaaaca accagtccct gtgatacaac tttgaaaaaa cttttaaaaa
     5281 tctctgatgt gtgggctctt tttttcccat aagaattatg tacatctgtg atgttttaca
     5341 gggggatccg cttttaaaca gtgtacatat tggaccacac tgaaatgtca tatatccttt
     5401 ctctacttaa aattggttat ttactgtgag ttcatttccg atgtgttctt ggttgttgct
     5461 gttttctgcc tgaagacgtg tatcataaag agctacatgg tatggactat ttatttgtaa
     5521 tttgacataa agtttgaaga ataaagatct ataaaagttg aagtttctga tattaaa
//
