LOCUS       NM_025149               2177 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens acyl-CoA synthetase family member 2 (ACSF2),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_025149
VERSION     NM_025149.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Chen,J., Feng,Q., Qiao,Y., Pan,S., Liang,L., Liu,Y., Zhang,X.,
            Liu,D., Liu,Z. and Liu,Z.
  TITLE     ACSF2 and lysine lactylation contribute to renal tubule injury in
            diabetes
  JOURNAL   Diabetologia 67 (7), 1429-1443 (2024)
   PUBMED   38676722
  REMARK    GeneRIF: ACSF2 and lysine lactylation contribute to renal tubule
            injury in diabetes.
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 2177)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 2177)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2177)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2177)
  AUTHORS   Yu,H., Tardivo,L., Tam,S., Weiner,E., Gebreab,F., Fan,C.,
            Svrzikapa,N., Hirozane-Kishikawa,T., Rietman,E., Yang,X.,
            Sahalie,J., Salehi-Ashtiani,K., Hao,T., Cusick,M.E., Hill,D.E.,
            Roth,F.P., Braun,P. and Vidal,M.
  TITLE     Next-generation sequencing to generate interactome datasets
  JOURNAL   Nat Methods 8 (6), 478-480 (2011)
   PUBMED   21516116
REFERENCE   7  (bases 1 to 2177)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2177)
  AUTHORS   Watkins,P.A., Maiguel,D., Jia,Z. and Pevsner,J.
  TITLE     Evidence for 26 distinct acyl-coenzyme A synthetase genes in the
            human genome
  JOURNAL   J Lipid Res 48 (12), 2736-2750 (2007)
   PUBMED   17762044
REFERENCE   9  (bases 1 to 2177)
  AUTHORS   Perera,R.J., Marcusson,E.G., Koo,S., Kang,X., Kim,Y., White,N. and
            Dean,N.M.
  TITLE     Identification of novel PPARgamma target genes in primary human
            adipocytes
  JOURNAL   Gene 369, 90-99 (2006)
   PUBMED   16380219
REFERENCE   10 (bases 1 to 2177)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK300625.1, AY358660.1 and AK308584.1.
            
            On Feb 27, 2020 this sequence version replaced NM_025149.5.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            5' coding region, compared to variant 1. The encoded isoform (2) is
            shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014123.2, AY358660.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000300441.9/
                                                  ENSP00000300441.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AK300625.1         61-76
            17-2175             AY358660.1         13-2171
            2176-2177           AK308584.1         855-856
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..2177
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /note="acyl-CoA synthetase family member 2"
                     /db_xref="GeneID:80221"
                     /db_xref="HGNC:HGNC:26101"
                     /db_xref="MIM:610465"
     exon            1..172
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     CDS             45..1892
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /EC_number="6.2.1.2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; PPARG binding, long chain fatty acid acyl Co-A
                     ligase like; acyl-CoA synthetase family member 2,
                     mitochondrial; medium-chain acyl-CoA ligase ACSF2,
                     mitochondrial"
                     /codon_start=1
                     /product="medium-chain acyl-CoA ligase ACSF2,
                     mitochondrial isoform 2 precursor"
                     /protein_id="NP_079425.3"
                     /db_xref="CCDS:CCDS11567.1"
                     /db_xref="GeneID:80221"
                     /db_xref="HGNC:HGNC:26101"
                     /db_xref="MIM:610465"
                     /translation="MAVYVGMLRLGRLCAGSSGVLGARAALSRSWQEARLQGVRFLSS
                     REVDRMVSTPIGGLSYVQGCTKKHLNSKTVGQCLETTAQRVPEREALVVLHEDVRLTF
                     AQLKEEVDKAASGLLSIGLCKGDRLGMWGPNSYAWVLMQLATAQAGIILVSVNPAYQA
                     MELEYVLKKVGCKALVFPKQFKTQQYYNVLKQICPEVENAQPGALKSQRLPDLTTVIS
                     VDAPLPGTLLLDEVVAAGSTRQHLDQLQYNQQFLSCHDPINIQFTSGTTGSPKGATLS
                     HYNIVNNSNILGERLKLHEKTPEQLRMILPNPLYHCLGSVAGTMMCLMYGATLILASP
                     IFNGKKALEAISRERGTFLYGTPTMFVDILNQPDFSSYDISTMCGGVIAGSPAPPELI
                     RAIINKINMKDLVVAYGTTENSPVTFAHFPEDTVEQKAESVGRIMPHTEARIMNMEAG
                     TLAKLNTPGELCIRGYCVMLGYWGEPQKTEEAVDQDKWYWTGDVATMNEQGFCKIVGR
                     SKDMIIRGGENIYPAELEDFFHTHPKVQEVQVVGVKDDRMGEEICACIRLKDGEETTV
                     EEIKAFCKGKISHFKIPKYIVFVTNYPLTISGKIQKFKLREQMERHLNL"
     exon            173..368
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            369..497
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            498..551
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            552..670
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            671..836
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            837..932
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1090
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1182
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1259
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1367
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1519
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1661
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1772
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1841
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     exon            1842..2177
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2152..2157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /note="hexamer: CATAAA"
     polyA_site      2177
                     /gene="ACSF2"
                     /gene_synonym="ACSMW; AVYV493"
                     /note="major polyA site"
ORIGIN      
        1 actttaaaag tttactcggg ccgggacgca gggcaaagcg agccatggct gtctacgtcg
       61 ggatgctgcg cctggggagg ctgtgcgccg ggagctcggg ggtgctgggg gcccgggccg
      121 ccctctctcg gagttggcag gaagccaggt tgcagggtgt ccgcttcctc agttccagag
      181 aggtggatcg catggtctcc acgcccatcg gaggcctcag ctacgttcag gggtgcacca
      241 aaaagcatct taacagcaag actgtgggcc agtgcctgga gaccacagca cagagggtcc
      301 cagaacgaga ggccttggtc gtcctccatg aagacgtcag gttgaccttt gcccaactca
      361 aggaggaggt ggacaaagct gcttctggcc tcctgagcat tggcctctgc aaaggtgacc
      421 ggctgggcat gtggggacct aactcctatg catgggtgct catgcagttg gccaccgccc
      481 aggcgggcat cattctggtg tctgtgaacc cagcctacca ggctatggaa ctggagtatg
      541 tcctcaagaa ggtgggctgc aaggcccttg tgttccccaa gcaattcaag acccagcaat
      601 actacaacgt cctgaagcag atctgtccag aagtggagaa tgcccagcca ggggccttga
      661 agagtcagag gctcccagat ctgaccacag tcatctcggt ggatgcccct ttgccgggga
      721 ccctgctcct ggatgaagtg gtggcggctg gcagcacacg gcagcatctg gaccagctcc
      781 aatacaacca gcagttcctg tcctgccatg accccatcaa catccagttc acctcgggga
      841 caacaggcag ccccaagggg gccaccctct cccactacaa cattgtcaac aactccaaca
      901 ttttaggaga gcgcctgaaa ctgcatgaga agacaccaga gcagttgcgg atgatcctgc
      961 ccaaccccct gtaccattgc ctgggttccg tggcaggcac aatgatgtgt ctgatgtacg
     1021 gtgccaccct catcctggcc tctcccatct tcaatggcaa gaaggcactg gaggccatca
     1081 gcagagagag aggcaccttc ctgtatggta cccccacgat gttcgtggac attctgaacc
     1141 agccagactt ctccagttat gacatctcga ccatgtgtgg aggtgtcatt gctgggtccc
     1201 ctgcacctcc agagttgatc cgagccatca tcaacaagat aaatatgaag gacctggtgg
     1261 ttgcttatgg aaccacagag aacagtcccg tgacattcgc gcacttccct gaggacactg
     1321 tggagcagaa ggcagaaagc gtgggcagaa ttatgcctca cacggaggcc cggatcatga
     1381 acatggaggc agggacgctg gcaaagctga acacgcccgg ggagctgtgc atccgagggt
     1441 actgcgtcat gctgggctac tggggtgagc ctcagaagac agaggaagca gtggatcagg
     1501 acaagtggta ttggacagga gatgtcgcca caatgaatga gcagggcttc tgcaagatcg
     1561 tgggccgctc taaggatatg atcatccggg gtggtgagaa catctacccc gcagagctcg
     1621 aggacttctt tcacacacac ccgaaggtgc aggaagtgca ggtggtggga gtgaaggacg
     1681 atcggatggg ggaagagatt tgtgcctgca ttcggctgaa ggacggggag gagaccacgg
     1741 tggaggagat aaaagctttc tgcaaaggga agatctctca cttcaagatt ccgaagtaca
     1801 tcgtgtttgt cacaaactac cccctcacca tttcaggaaa gatccagaaa ttcaaacttc
     1861 gagagcagat ggaacgacat ctaaatctgt gaataaagca gcaggcctgt cctggccggt
     1921 tggcttgact ctctcctgtc agaatgcaac ctggctttat gcacctagat gtccccagca
     1981 cccagttctg agccaggcac atcaaatgtc aaggaattga ctgaacgaac taagagctcc
     2041 tggatgggtc cgggaactcg cctgggcaca aggtgccaaa aggcaggcag cctgcccagg
     2101 ccctccctcc tgtccatccc ccacattccc ctgtctgtcc ttgtgatttg gcataaagag
     2161 cttctgtttt ctttggc
//
