LOCUS       NM_004035               7317 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens acyl-CoA oxidase 1 (ACOX1), transcript variant 1,
            mRNA.
ACCESSION   NM_004035
VERSION     NM_004035.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Thiels,C., Lucke,T., Rothoeft,T., Lukas,C., Nguyen,H.P., von
            Kleist-Retzow,J.C., Prokisch,H., Grimmel,M., Haack,T.B. and
            Hoffjan,S.
  TITLE     ACOX1 Gain-of-Function Variant in Two German Pediatric Patients, in
            One Case Mimicking Autoimmune Inflammatory Disease
  JOURNAL   Neuropediatrics 55 (2), 140-145 (2024)
   PUBMED   37846133
  REMARK    GeneRIF: ACOX1 Gain-of-Function Variant in Two German Pediatric
            Patients, in One Case Mimicking Autoimmune Inflammatory Disease.
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Tannoury,M., Ayoub,M., Dehgane,L., Nemazanyy,I., Dubois,K.,
            Izabelle,C., Brousse,A., Roos-Weil,D., Maloum,K., Merle-Beral,H.,
            Bauvois,B., Saubamea,B., Chapiro,E., Nguyen-Khac,F., Garnier,D. and
            Susin,S.A.
  TITLE     ACOX1-mediated peroxisomal fatty acid oxidation contributes to
            metabolic reprogramming and survival in chronic lymphocytic
            leukemia
  JOURNAL   Leukemia 38 (2), 302-317 (2024)
   PUBMED   38057495
  REMARK    GeneRIF: ACOX1-mediated peroxisomal fatty acid oxidation
            contributes to metabolic reprogramming and survival in chronic
            lymphocytic leukemia.
REFERENCE   3  (bases 1 to 7317)
  AUTHORS   Ding,J., Xia,C., Cen,P., Li,S., Yu,L., Zhu,J. and Jin,J.
  TITLE     MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic
            fatty liver disease by targeting of ACOX1
  JOURNAL   Mol Biol Rep 49 (8), 7297-7305 (2022)
   PUBMED   35606603
  REMARK    GeneRIF: MiR-103-3p promotes hepatic steatosis to aggravate
            nonalcoholic fatty liver disease by targeting of ACOX1.
REFERENCE   4  (bases 1 to 7317)
  AUTHORS   Sonani,R.R., Blat,A. and Dubin,G.
  TITLE     Crystal structures of apo- and FAD-bound human peroxisomal acyl-CoA
            oxidase provide mechanistic basis explaining clinical observations
  JOURNAL   Int J Biol Macromol 205, 203-210 (2022)
   PUBMED   35149097
  REMARK    GeneRIF: Crystal structures of apo- and FAD-bound human peroxisomal
            acyl-CoA oxidase provide mechanistic basis explaining clinical
            observations.
REFERENCE   5  (bases 1 to 7317)
  AUTHORS   Vluggens,A., Andreoletti,P., Viswakarma,N., Jia,Y., Matsumoto,K.,
            Kulik,W., Khan,M., Huang,J., Guo,D., Yu,S., Sarkar,J., Singh,I.,
            Rao,M.S., Wanders,R.J., Reddy,J.K. and Cherkaoui-Malki,M.
  TITLE     Reversal of mouse Acyl-CoA oxidase 1 (ACOX1) null phenotype by
            human ACOX1b isoform [corrected]
  JOURNAL   Lab Invest 90 (5), 696-708 (2010)
   PUBMED   20195242
  REMARK    GeneRIF: Data show that human ACOX1b isoform is more effective than
            the ACOX1a isoform in reversing the Acox1 null phenotype in the
            mouse.
            Erratum:[Lab Invest. 2010 May;90(5):808]
REFERENCE   6  (bases 1 to 7317)
  AUTHORS   Oaxaca-Castillo,D., Andreoletti,P., Vluggens,A., Yu,S., van
            Veldhoven,P.P., Reddy,J.K. and Cherkaoui-Malki,M.
  TITLE     Biochemical characterization of two functional human liver acyl-CoA
            oxidase isoforms 1a and 1b encoded by a single gene
  JOURNAL   Biochem Biophys Res Commun 360 (2), 314-319 (2007)
   PUBMED   17603022
REFERENCE   7  (bases 1 to 7317)
  AUTHORS   Watkins,P.A., McGuinness,M.C., Raymond,G.V., Hicks,B.A., Sisk,J.M.,
            Moser,A.B. and Moser,H.W.
  TITLE     Distinction between peroxisomal bifunctional enzyme and acyl-CoA
            oxidase deficiencies
  JOURNAL   Ann Neurol 38 (3), 472-477 (1995)
   PUBMED   7668838
REFERENCE   8  (bases 1 to 7317)
  AUTHORS   Chu,R., Varanasi,U., Chu,S., Lin,Y., Usuda,N., Rao,M.S. and
            Reddy,J.K.
  TITLE     Overexpression and characterization of the human peroxisomal
            acyl-CoA oxidase in insect cells
  JOURNAL   J Biol Chem 270 (9), 4908-4915 (1995)
   PUBMED   7876265
REFERENCE   9  (bases 1 to 7317)
  AUTHORS   Vanderver,A., Tonduti,D., Schiffmann,R., Schmidt,J. and van der
            Knaap,M.S.
  TITLE     Leukodystrophy Overview - RETIRED CHAPTER, FOR HISTORICAL REFERENCE
            ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24501781
REFERENCE   10 (bases 1 to 7317)
  AUTHORS   Singh,H., Brogan,M., Johnson,D. and Poulos,A.
  TITLE     Peroxisomal beta-oxidation of branched chain fatty acids in human
            skin fibroblasts
  JOURNAL   J Lipid Res 33 (11), 1597-1605 (1992)
   PUBMED   1464743
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC008767.2, BQ943492.1 and
            AC087289.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004035.6.
            
            Summary: The protein encoded by this gene is the first enzyme of
            the fatty acid beta-oxidation pathway, which catalyzes the
            desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates
            electrons directly to molecular oxygen, thereby producing hydrogen
            peroxide. Defects in this gene result in pseudoneonatal
            adrenoleukodystrophy, a disease that is characterized by
            accumulation of very long chain fatty acids. Alternatively spliced
            transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform a, alternately
            referred to as ACOX1b per PMID: 17603022.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.145496.1,
                                           SRR1803617.245101.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000293217.10/ ENSP00000293217.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1028              BC008767.2         198-1225
            1029-1377           BQ943492.1         506-854
            1378-3243           BC008767.2         1575-3440
            3244-7317           AC087289.9         7807-11880
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..7317
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /note="acyl-CoA oxidase 1"
                     /db_xref="GeneID:51"
                     /db_xref="HGNC:HGNC:119"
                     /db_xref="MIM:609751"
     exon            1..202
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..2076
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /EC_number="1.3.3.6"
                     /note="isoform a is encoded by transcript variant 1;
                     acyl-Coenzyme A oxidase 1, palmitoyl; peroxisomal fatty
                     acyl-CoA oxidase; acyl-CoA oxidase, straight-chain;
                     peroxisomal acyl-coenzyme A oxidase 1; palmitoyl-CoA
                     oxidase; straight-chain acyl-CoA oxidase; acyl-CoA oxidase
                     1, palmitoyl"
                     /codon_start=1
                     /product="peroxisomal acyl-coenzyme A oxidase 1 isoform a"
                     /protein_id="NP_004026.2"
                     /db_xref="CCDS:CCDS11734.1"
                     /db_xref="GeneID:51"
                     /db_xref="HGNC:HGNC:119"
                     /db_xref="MIM:609751"
                     /translation="MNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDP
                     DFQHEDLNFLTRSQRYEVAVRKSAIMVKKMREFGIADPDEIMWFKNFVHRGRPEPLDL
                     HLGMFLPTLLHQATAEQQERFFMPAWNLEIIGTYAQTEMGHGTHLRGLETTATYDPET
                     QEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIREIGTHKPL
                     PGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLSNKLTYG
                     TMVFVRSFLVGEAARALSKACTIAIRYSAVRHQSEIKPGEPEPQILDFQTQQYKLFPL
                     LATAYAFQFVGAYMKETYHRINEGIGQGDLSELPELHALTAGLKAFTSWTANTGIEAC
                     RMACGGHGYSHCSGLPNIYVNFTPSCTFEGENTVMMLQTARFLMKSYDQVHSGKLVCG
                     MVSYLNDLPSQRIQPQQVAVWPTMVDINSPESLTEAYKLRAARLVEIAAKNLQKEVIH
                     RKSKEVAWNLTSVDLVRASEAHCHYVVVKLFSEKLLKIQDKAIQAVLRSLCLLYSLYG
                     ISQNAGDFLQGSIMTEPQITQVNQRVKELLTLIRSDAVALVDAFDFQDVTLGSVLGRY
                     DGNVYENLFEWAKNSPLNKAEVHESYKHLKSLQSKL"
     exon            203..362
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            363..523
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            524..631
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            632..751
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            752..867
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1037
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1200
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1391
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1571
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1677
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1821
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..2028
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..7317
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5927..5932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /note="hexamer: AATAAA"
     polyA_site      5946
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /note="major polyA site"
     regulatory      7284..7289
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /note="hexamer: AATAAA"
     polyA_site      7317
                     /gene="ACOX1"
                     /gene_synonym="ACOX; AOX; MITCH; PALMCOX; SCOX"
                     /note="major polyA site"
ORIGIN      
        1 gcctcctagg gtccggccgg cgctggagct gcggatttag attgtcactg ccacctcggt
       61 cggtgcttac ttcgctgcca gctggtcgtc gccatgaacc cggacctgcg cagggagcgg
      121 gattccgcca gcttcaaccc ggagctgctt acacacatcc tggacggcag ccccgagaaa
      181 acccggcgcc gccgagagat cgagaacatg atcctgaacg acccagactt ccagcatgag
      241 gacttgaact tcctcactcg cagccagcgt tatgaggtgg ctgtcaggaa aagtgccatc
      301 atggtgaaga agatgaggga gtttggcatc gctgaccctg atgaaattat gtggtttaaa
      361 aattttgtgc accgagggcg gcctgagcct ctggatcttc acttgggcat gttcctgccc
      421 accttgcttc accaggcaac tgcggagcag caggagcgct tcttcatgcc cgcctggaac
      481 ttggagatca ttggcactta tgcccagaca gagatgggtc atggaactca ccttcgaggc
      541 ttggaaacca cagccacgta tgaccctgaa acccaggagt tcattctcaa cagtcctact
      601 gtgacctcca ttaaatggtg gcctggtggg cttggaaaga cttcaaatca tgcaatagtt
      661 cttgcccagc tcatcactaa ggggaaatgc tatggattac atgcctttat cgtacctatt
      721 cgtgaaatcg ggacccataa gcctttgcca ggaattaccg ttggtgacat cggccccaaa
      781 tttggttatg atgagataga caatggctac ctcaaaatgg acaaccatcg tattcccaga
      841 gaaaacatgc tgatgaagta tgcccaggtg aagcctgatg gcacatacgt gaaaccgctg
      901 agtaacaagc tgacttacgg gaccatggtg tttgtcaggt ccttccttgt gggagaagct
      961 gctcgggctc tgtctaaggc gtgcaccatt gccatccgat acagcgctgt gaggcaccag
     1021 tctgaaatca agccaggtga accagaacca cagattttgg attttcaaac ccagcagtat
     1081 aaactctttc cactcctggc cactgcctat gccttccagt ttgtgggcgc atacatgaag
     1141 gagacctatc accggattaa cgaaggcatt ggtcaagggg acctgagtga actgcctgag
     1201 cttcatgccc tcaccgctgg actgaaggct ttcacctcct ggactgcaaa cactggcatt
     1261 gaagcatgtc ggatggcttg tggtgggcat ggctattctc attgcagtgg tcttccaaat
     1321 atttatgtca atttcacccc aagctgtacc tttgagggag aaaacactgt catgatgctc
     1381 cagacggcta ggttcctgat gaaaagttat gatcaggtgc actcaggaaa gttggtgtgt
     1441 ggcatggtgt cctatttgaa cgacctgccc agtcagcgca tccagccaca gcaggtagca
     1501 gtctggccaa ccatggtgga tatcaacagc cccgaaagcc taaccgaagc atataaactc
     1561 cgtgcagcca gattagtaga aattgctgca aaaaaccttc aaaaagaagt gattcacaga
     1621 aaaagcaagg aggtagcttg gaacctaact tctgttgacc ttgttcgagc aagtgaggca
     1681 cattgccact atgtggtagt taagctcttt tcagaaaaac tcctcaaaat tcaagataaa
     1741 gccattcaag ctgtcttaag gagtttatgt ctgctgtatt ctctgtatgg aatcagtcag
     1801 aacgcggggg atttccttca ggggagcatc atgacagagc ctcagattac acaagtaaac
     1861 cagcgtgtaa aggagttact cactctgatt cgctcagatg ctgttgcttt ggttgatgca
     1921 tttgattttc aggatgtgac acttggctct gtgcttggcc gctatgatgg gaatgtgtat
     1981 gaaaacttgt ttgagtgggc taagaactcc ccactgaaca aagcagaggt ccacgaatct
     2041 tacaagcacc tgaagtcact gcagtccaag ctctgaagtg tcacaaggac aagtttaatc
     2101 tgcttcagaa agcgcctgtg tgcaactcaa attttgtgga atctttttcg aattcaaata
     2161 gctatagagc aaatgataaa ttgacccctt tttataaatg gagggaaaaa atgaacagat
     2221 ttcagagatt aaatgaaaaa aagcagatgt tttaagtgca attaacactg aaagagacct
     2281 gttaaaccat tcagaaaaag cttaagaaat gcgatatgac ttccttttgt aatgctgctg
     2341 atcccagtag actatgactt ttgataatta gcagaattta actactgagt agttgattat
     2401 tttcacattt taattgctaa tcactggcta tataagtgtt tttaagcaaa ggtatttttg
     2461 aagtggtgta gaacccttcc aagctttcct gctcagtgtt ctaccagact taccctgggg
     2521 cctggcttaa aagcaggatt gaagaaaagg gactggggga aggaaactta ttggaaaact
     2581 tgatgcgaat gagtttctgc ttggcacagt ctctgcctgc ttgctctcct ttgctgatgg
     2641 attgcattta tcaaactatt catgctagca tttttccaac gagggaactt attccgcacg
     2701 ggcctactgt aggaccattg tctcgtgtaa ttaggaattt tccatttgaa ggattgctaa
     2761 attgtcacag tagtaggaag tatagggaaa cctctcagct gtggcactgt tgtagctttg
     2821 gagtgcagag tgtaactctg ggacaatcag atttcacata ttctgtcatc ttggcataag
     2881 ccattaaaag cttggagatt actgtatttg gcattaaaaa aaaatgtcac ttaggtcagc
     2941 actcccagac gtagcacaga aaaacccttt gacacaaacc atgtgttctg atttttggtt
     3001 cagaaaatat tgaaactgtg agttgttttt tttttaacaa ctgggaaaaa acaaaaacaa
     3061 aaaactatag ttagaaaaat ggaagttcca taggttctat ttcttactct atgtatggct
     3121 ttgttttcag tctatttcta ggagctttct ctgaatcgct aattgtcctt tcagttgaaa
     3181 tctaatttat acaatcattc tatacttaaa ggttaaatac atcttaatta attttttctt
     3241 aaagtcaatg taagtcactt tgttttgttt ttttttaatc tacgccatat gcctcatgaa
     3301 accagctgtt ctagaatcag tcctgagaat atggcttaat tccatggaaa cataactcct
     3361 atcttgggac ctgacataat atctatctat cctggggaac tggtaatatg agacttatag
     3421 gttacagcag aaatgctaca tgttgacaaa agccttaatc gttccactgg gagaactaat
     3481 tgataattgt gttaagattg aagattaacc ctgtgttaat ctcacttgag tctatcctga
     3541 cagtagttca gattctggaa aatgataaac tgacctgcta gatgtagaat tgtttcaaaa
     3601 ttagtgttga aataccttgt tcacagatga atatctgggc aggatctgag ggtgtttgga
     3661 atgacacccc ccaatccagt tgcatagatg ggatgtcttt gcaggtttga ggagatcatc
     3721 gacctgcaga gccccctttg acccagtacc tcacgtttta tttaaaatct aaatctgggg
     3781 ccaggcgtgg tggctcacgc ctgtaatcca agcactttgg gaggccgaag cgggtggatc
     3841 acctgaggtc aggagtttga gaccatcctg gccaacatgg tgaaaccccg tctctactga
     3901 aaaaaataca aaaattagct gggcatggtg gtagcacgcg cctgtagtcc cagctactcg
     3961 ggaggctgag gcaggataat cgcttgaact tggcgggtag aggttgcagt gagccaagat
     4021 ggtgccacta cactgcagcc tgggtggctg agcgagactc cgtctcaaaa aaaaaatcta
     4081 aatctgacat ttgatgctat ttttattaat attggaatgt tctgtcttga actttattca
     4141 atataatcaa gaataaagat agagtaaacg tcactgattt gtactattaa gagagaaaaa
     4201 atatgccaca caactaaaca taggtttaaa ttatgaagaa atttagaata gaggtttatt
     4261 agatttaggg aacactaaga acaaaaaagg aaggagtgat acctgcctga gtggacagct
     4321 gtaaatcagc tgtaattact gcagttgtac caatagttgt gagtggctcc agtcacttta
     4381 ggagtccttg gaagtacttg gtacacattt gttggctgta ccttaaagga agtggcaagt
     4441 ccagtttgtt ctctctacca cactagactg ccactgacaa gtttgggtct gttggattca
     4501 aaattttgta agccattttc acaagtacaa agatacattt taaccttgtc ttctccaaaa
     4561 ttactgagta ggaattttat ttttatcttt ttgagacagg gtatcactgt cacccagact
     4621 ggagtgcagt ggtgggatct tggcttactg tgacctctgc ctcccgggtt caaatggtcc
     4681 tccctcctca gtctcctgag tagctgggac gacaggcacg tgccaccatg cccagctaat
     4741 ttgttctatt ttttctgtag agacggggtt ttgccatgtt gcccaggctg gtctcagact
     4801 cctgggctca agcgatcatt tcgcctcagc ctcccaaagt gctgagatta taggtgtgag
     4861 ccacagcatc tggcccagag tgaggagaat taatgagatt ttttgtgtgt gttagataat
     4921 attgatttaa gccttttttt aaaaagtact ctcaaccaaa tacaaaattg aaaatgtgag
     4981 gtttaataga aatgtgttgg ctatttgcaa tggattttct tcttgcccaa gtgtttggag
     5041 ttctacttta tgctctgtat ttaaaaatta gtgacctcaa agcagagttg atgacacagg
     5101 ctttggggcc ctcatgttct tgtctttaga acatatcact actaagtatc agcttatctt
     5161 cagaacatta caacattcac cgtgttcata tgctttctga gaagtcacca cttgtaattt
     5221 cagatcacat acacctgaag gcattttata gttcctaaag ttaacatgtt agatcttttt
     5281 tttccacccc atgagggtct cactctcacc caggctggaa tgcagtggtg tgattgtagc
     5341 acactttggc caccaactcc tgggctcaag tgatcctcct gctttggcct cctctgagaa
     5401 gctgggatta ctggtgcaca ccaccacacc tggctaattt tttatttttt tttatttttg
     5461 gaaatagggt atggctatgt tgccttgggc ccgtcatgaa ctcctagcct caggtgaccc
     5521 tcccacctca ggcctcccaa agggttggga ttacaggagt gagctactgc actgggccaa
     5581 catgttaatt tttttttttt tttttttgag atggagtctc ccaggcggga atgcaatggt
     5641 gcgatctcga cttcactgca atcttcacct cccgggttcg agcgattctc ccacctcagc
     5701 ctcctgagta gctgggatta caggcacctg ccatcacgct cggctaattt ttgtattttg
     5761 agcagagatg gagtttcacc atgttggcca ggtgatctgc actcccactc cccccacact
     5821 tcccaaagtg ctgggattac aggcataagc cacaagccac ctcacccagc caacatgtta
     5881 catcttaatt cttggatttt cttcactgca gggctttggg tggagaaata aaactcttca
     5941 aatgcatgat cttggagatc cctgtgaatc aataattctt tagacaactg cggctcaaaa
     6001 tccctccttt cccttttccg agttattcca tccatcttat tagaaaggaa gtgaattagg
     6061 tgtaggtggt ctgtaacacc tgcacatctt tttatacgtg tagagggtat gcctgggata
     6121 tataggttgt cttcaagcag tagctgctac tacagctaga gagaggagga gtgccaggaa
     6181 actgatgacc tgagaccaag agtcttgttg atgttctgac ttagataaag gttttgatca
     6241 ttttcatgaa ataatgcagg gaagtcattt ctgctgtttc tttactactc cattcttgga
     6301 ggattagaac aagtcacact gtaattgact aaaacgactt tttattttaa aatattgatg
     6361 gtggggtttt gctttttttt tttttttttt ttttgagacg ttgtcttgct ctttcaccca
     6421 gggtagagtg cagtggcgaa atcttggctc actgcaacct ctgcctcccg ggttcaagca
     6481 attctctgcc tcagcctcct gagaagctgg gattacagcg cctgccacca tgcctgtcta
     6541 gtttttttgt atttttagta gagacagggg tttcaccaag ttggccaggc tggtcttgaa
     6601 ctcctgacct cgtgatccac ccacctcggc ctcccaaagt gctgggatta caggcgtgag
     6661 ccaccgtgcc gggccagtgt ttttaactgt ccacacttac taaattttcc agtacttctt
     6721 cctatggttc atgatagtaa acacagaaac atataaggaa cattattaga gttaccagat
     6781 gttagctgtt gcatatgtgc acattcctag attcaagggt gtttttgcct taaatttaag
     6841 tgggtcattg gttgtcttgg gaagatcatg gaaaattcgg gattttttag aattctgaac
     6901 caaaatatgt tttgagtgtt tcttagtaaa tgtgtgatct tccaccttcc acattcagac
     6961 tgcggactac acttcataaa tgccttttta tttccagtta tggattcaac taaatgactg
     7021 ccttgggagc acataattac tttgctacct ttttccccct ttgctgttgt ggctcgagtt
     7081 tggttctcac ctgagaagat gcattgagca tatgttgtta cccagccctg gcttaatggt
     7141 gtcctgtggg gtaggggtgg gaggacgagg ggcacggggc cagagcatgt gaatggatca
     7201 tggttggaca gctgtgacct gccagcactg cgggtaagca aaactacaaa ccgttctttc
     7261 ctctgtgaca ttgaataaac cttaataaaa ttcataatta gcacatacta gaaaaaa
//
