LOCUS       NM_152331               1465 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens acyl-CoA thioesterase 4 (ACOT4), mRNA.
ACCESSION   NM_152331
VERSION     NM_152331.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Ni,C., Zheng,K., Gao,Y., Chen,Y., Shi,K., Ni,C., Jin,G. and Yu,G.
  TITLE     ACOT4 accumulation via AKT-mediated phosphorylation promotes
            pancreatic tumourigenesis
  JOURNAL   Cancer Lett 498, 19-30 (2021)
   PUBMED   33148467
  REMARK    GeneRIF: ACOT4 accumulation via AKT-mediated phosphorylation
            promotes pancreatic tumourigenesis.
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Auer,P.L., Nalls,M., Meschia,J.F., Worrall,B.B., Longstreth,W.T.
            Jr., Seshadri,S., Kooperberg,C., Burger,K.M., Carlson,C.S.,
            Carty,C.L., Chen,W.M., Cupples,L.A., DeStefano,A.L., Fornage,M.,
            Hardy,J., Hsu,L., Jackson,R.D., Jarvik,G.P., Kim,D.S.,
            Lakshminarayan,K., Lange,L.A., Manichaikul,A., Quinlan,A.R.,
            Singleton,A.B., Thornton,T.A., Nickerson,D.A., Peters,U. and
            Rich,S.S.
  CONSRTM   National Heart, Lung, and Blood Institute Exome Sequencing Project
  TITLE     Rare and Coding Region Genetic Variants Associated With Risk of
            Ischemic Stroke: The NHLBI Exome Sequence Project
  JOURNAL   JAMA Neurol 72 (7), 781-788 (2015)
   PUBMED   25961151
  REMARK    GeneRIF: Exome sequencing revealed an association with ACOT4 and
            increased risk of ischemic stroke.
REFERENCE   3  (bases 1 to 1465)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   4  (bases 1 to 1465)
  AUTHORS   Hunt,M.C., Rautanen,A., Westin,M.A., Svensson,L.T. and Alexson,S.E.
  TITLE     Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene
            clusters shows that convergent, functional evolution results in a
            reduced number of human peroxisomal ACOTs
  JOURNAL   FASEB J 20 (11), 1855-1864 (2006)
   PUBMED   16940157
REFERENCE   5  (bases 1 to 1465)
  AUTHORS   Westin,M.A., Hunt,M.C. and Alexson,S.E.
  TITLE     The identification of a succinyl-CoA thioesterase suggests a novel
            pathway for succinate production in peroxisomes
  JOURNAL   J Biol Chem 280 (46), 38125-38132 (2005)
   PUBMED   16141203
  REMARK    GeneRIF: ACOT4 and ACOT8 are responsible for the termination of
            beta-oxidation of dicarboxylic acids of medium-chain length with
            the concomitant release of the corresponding free acids
REFERENCE   6  (bases 1 to 1465)
  AUTHORS   Hunt,M.C., Yamada,J., Maltais,L.J., Wright,M.W., Podesta,E.J. and
            Alexson,S.E.
  TITLE     A revised nomenclature for mammalian acyl-CoA
            thioesterases/hydrolases
  JOURNAL   J Lipid Res 46 (9), 2029-2032 (2005)
   PUBMED   16103133
REFERENCE   7  (bases 1 to 1465)
  AUTHORS   Jones,J.M. and Gould,S.J.
  TITLE     Identification of PTE2, a human peroxisomal long-chain acyl-CoA
            thioesterase
  JOURNAL   Biochem Biophys Res Commun 275 (1), 233-240 (2000)
   PUBMED   10944470
REFERENCE   8  (bases 1 to 1465)
  AUTHORS   Hunt,M.C., Nousiainen,S.E., Huttunen,M.K., Orii,K.E., Svensson,L.T.
            and Alexson,S.E.
  TITLE     Peroxisome proliferator-induced long chain acyl-CoA thioesterases
            comprise a highly conserved novel multi-gene family involved in
            lipid metabolism
  JOURNAL   J Biol Chem 274 (48), 34317-34326 (1999)
   PUBMED   10567408
REFERENCE   9  (bases 1 to 1465)
  AUTHORS   Sherrington,R., Rogaev,E.I., Liang,Y., Rogaeva,E.A., Levesque,G.,
            Ikeda,M., Chi,H., Lin,C., Li,G., Holman,K., Tsuda,T., Mar,L.,
            Foncin,J.F., Bruni,A.C., Montesi,M.P., Sorbi,S., Rainero,I.,
            Pinessi,L., Nee,L., Chumakov,I., Pollen,D., Brookes,A., Sanseau,P.,
            Polinsky,R.J., Wasco,W., Da Silva,H.A., Haines,J.L.,
            Perkicak-Vance,M.A., Tanzi,R.E., Roses,A.D., Fraser,P.E.,
            Rommens,J.M. and St George-Hyslop,P.H.
  TITLE     Cloning of a gene bearing missense mutations in early-onset
            familial Alzheimer's disease
  JOURNAL   Nature 375 (6534), 754-760 (1995)
   PUBMED   7596406
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC005225.2.
            
            On Jul 31, 2020 this sequence version replaced NM_152331.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090945.1, AK094223.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326303.5/ ENSP00000323071.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-544               AC005225.2         67951-68494         c
            545-747             AC005225.2         66463-66665         c
            748-1465            AC005225.2         64601-65318         c
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.3"
     gene            1..1465
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /note="acyl-CoA thioesterase 4"
                     /db_xref="GeneID:122970"
                     /db_xref="HGNC:HGNC:19748"
                     /db_xref="MIM:614314"
     exon            1..544
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1353
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /EC_number="3.1.2.2"
                     /EC_number="3.1.2.3"
                     /note="acyl-coenzyme A thioesterase 4; peroxisomal
                     acyl-CoA thioesterase 2B; PTE-2b; peroxisomal long-chain
                     acyl-CoA thioesterase Ib; peroxisomal acyl coenzyme A
                     thioester hydrolase Ib"
                     /codon_start=1
                     /product="peroxisomal succinyl-coenzyme A thioesterase"
                     /protein_id="NP_689544.3"
                     /db_xref="CCDS:CCDS9817.1"
                     /db_xref="GeneID:122970"
                     /db_xref="HGNC:HGNC:19748"
                     /db_xref="MIM:614314"
                     /translation="MSATLILEPPGRCCWNEPVRIAVRGLAPEQRVTLRASLRDEKGA
                     LFRAHARYCADARGELDLERAPALGGSFAGLEPMGLLWALEPEKPFWRFLKRDVQIPF
                     VVELEVLDGHDPEPGRLLCQAQHERHFLPPGVRRQSVRAGRVRATLFLPPGPGPFPGI
                     IDIFGIGGGLLEYRASLLAGHGFATLALAYYNFEDLPNNMDNISLEYFEEAVCYMLQH
                     PQVKGPGIGLLGISLGADICLSMASFLKNVSATVSINGSGISGNTAINYKHSSIPPLG
                     YDLRRIKVAFSGLVDIVDIRNALVGGYKNPSMIPIEKAQGPILLIVGQDDHNWRSELY
                     AQTVSERLQAHGKEKPQIICYPGTGHYIEPPYFPLCPASLHRLLNKHVIWGGEPRAHS
                     KAQEDAWKQILAFFCKHLGGTQKTAVPKL"
     exon            545..747
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /inference="alignment:Splign:2.1.0"
     exon            748..1465
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1441..1446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /note="hexamer: ATTAAA"
     polyA_site      1465
                     /gene="ACOT4"
                     /gene_synonym="PTE-Ib; PTE1B; PTE2B"
                     /note="major polyA site"
ORIGIN      
        1 ggacgccgtc cggacattcg gcgcgcttgc cacgatcttg gacgggtctc gggcctcgac
       61 ctttgaattc cccgctccgg ctccaagatg tcagcaacgc tgatcctgga gcccccaggc
      121 cgctgctgct ggaacgagcc ggtgcgcatt gccgtgcgcg gcctggcccc ggagcagcgg
      181 gttacgctgc gcgcgtccct gcgcgacgag aagggcgcgc tcttccgggc ccacgcgcgc
      241 tactgcgccg acgcccgcgg cgagctggac ctggagcgcg cacccgcgct gggcggcagc
      301 ttcgcgggac tcgagcccat ggggctgctc tgggccctgg aacccgagaa gcctttttgg
      361 cgcttcctga agcgggacgt acagattcct tttgtcgtgg agttggaggt gctggacggc
      421 cacgaccccg agcctggacg gctgctgtgc caggcgcagc acgagcgcca cttcctcccg
      481 ccaggggtgc ggcgccagtc ggtgcgagcg ggccgggtgc gcgccacgct cttcctgccg
      541 ccaggacctg gacccttccc agggatcatt gacatctttg gtattggagg gggcctcttg
      601 gaatatcgag ccagcctcct tgctggccat ggctttgcca cgttggctct agcttattat
      661 aactttgaag atctccccaa taacatggac aacatatccc tggagtactt cgaagaagcc
      721 gtatgctaca tgcttcaaca tccccaggta aaaggcccag gcattgggct tttgggcatt
      781 tctctaggag ctgatatttg tctctcaatg gcctcattct tgaagaatgt ctcagccaca
      841 gtttccatca atggatctgg gatcagtggg aacacagcca tcaactataa gcacagtagc
      901 attccaccat tgggctatga cctgaggaga atcaaggtag ctttctcagg cctcgtggac
      961 attgtggata taaggaatgc tctcgtagga gggtacaaga accccagcat gattccaata
     1021 gagaaggccc aggggcccat cctgctcatt gttggtcagg atgaccataa ctggagaagt
     1081 gagttgtatg cccaaacagt ctctgaacgg ttacaggccc atggaaagga aaaaccccag
     1141 atcatctgtt accctgggac tgggcattac atcgagcctc cttacttccc cctgtgccca
     1201 gcttcccttc acagattact gaacaaacat gttatatggg gtggggagcc cagggctcat
     1261 tctaaggccc aggaagatgc ctggaagcaa attctagcct tcttctgcaa acacctggga
     1321 ggtacccaga aaacagctgt ccctaaattg taatgcattt gtctgttgtt gacatgagag
     1381 attcaagatc agattctagt gttcagtaac cctatgtgaa tcagatgtct cctggataac
     1441 attaaagcca tgtctttgtc attaa
//
