LOCUS       NM_001609               5848 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001609 XM_929314 XM_940521
VERSION     NM_001609.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Yoo,S.S., Do,S.K., Choi,J.E., Kang,H.G., Hong,M.J., Lee,J.H.,
            Lee,W.K., Do,Y.W., Lee,E.B., Park,J.E., Choi,S.H., Seo,H.,
            Lee,Y.H., Lee,J., Lee,S.Y., Cha,S.I., Kim,C.H. and Park,J.Y.
  TITLE     Lipid Metabolism Pathway Genes and Lung Cancer: ACADSB
            rs12220683G&gt;C Is Associated with Better Survival Outcome in
            Patients with Non-Small Cell Lung Cancer
  JOURNAL   Oncology 102 (1), 67-75 (2024)
   PUBMED   37527640
  REMARK    GeneRIF: Lipid Metabolism Pathway Genes and Lung Cancer: ACADSB
            rs12220683G>C Is Associated with Better Survival Outcome in
            Patients with Non-Small Cell Lung Cancer.
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 5848)
  AUTHORS   Lu,D., Yang,Z., Xia,Q., Gao,S., Sun,S., Luo,X., Li,Z., Zhang,X. and
            Li,X.
  TITLE     ACADSB regulates ferroptosis and affects the migration, invasion,
            and proliferation of colorectal cancer cells
  JOURNAL   Cell Biol Int 44 (11), 2334-2343 (2020)
   PUBMED   32776663
  REMARK    GeneRIF: ACADSB regulates ferroptosis and affects the migration,
            invasion, and proliferation of colorectal cancer cells.
REFERENCE   4  (bases 1 to 5848)
  AUTHORS   Porta,F., Chiesa,N., Martinelli,D. and Spada,M.
  TITLE     Clinical, biochemical, and molecular spectrum of
            short/branched-chain acyl-CoA dehydrogenase deficiency: two new
            cases and review of literature
  JOURNAL   J Pediatr Endocrinol Metab 32 (2), 101-108 (2019)
   PUBMED   30730842
  REMARK    GeneRIF: ACADSB mutation is associated with short/branched-chain
            acyl-CoA dehydrogenase deficiency.
            Review article
REFERENCE   5  (bases 1 to 5848)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   6  (bases 1 to 5848)
  AUTHORS   He,M., Burghardt,T.P. and Vockley,J.
  TITLE     A novel approach to the characterization of substrate specificity
            in short/branched chain Acyl-CoA dehydrogenase
  JOURNAL   J Biol Chem 278 (39), 37974-37986 (2003)
   PUBMED   12855692
  REMARK    GeneRIF: Differences between the rat and human enzyme at positions
            383, 222, and 220 alter substrate specificity without affecting
            substrate binding.
REFERENCE   7  (bases 1 to 5848)
  AUTHORS   Andresen,B.S., Christensen,E., Corydon,T.J., Bross,P., Pilgaard,B.,
            Wanders,R.J., Ruiter,J.P., Simonsen,H., Winter,V., Knudsen,I.,
            Schroeder,L.D., Gregersen,N. and Skovby,F.
  TITLE     Isolated 2-methylbutyrylglycinuria caused by short/branched-chain
            acyl-CoA dehydrogenase deficiency: identification of a new enzyme
            defect, resolution of its molecular basis, and evidence for
            distinct acyl-CoA dehydrogenases in isoleucine and valine
            metabolism
  JOURNAL   Am J Hum Genet 67 (5), 1095-1103 (2000)
   PUBMED   11013134
REFERENCE   8  (bases 1 to 5848)
  AUTHORS   Gibson,K.M., Burlingame,T.G., Hogema,B., Jakobs,C., Schutgens,R.B.,
            Millington,D., Roe,C.R., Roe,D.S., Sweetman,L., Steiner,R.D.,
            Linck,L., Pohowalla,P., Sacks,M., Kiss,D., Rinaldo,P. and
            Vockley,J.
  TITLE     2-Methylbutyryl-coenzyme A dehydrogenase deficiency: a new inborn
            error of L-isoleucine metabolism
  JOURNAL   Pediatr Res 47 (6), 830-833 (2000)
   PUBMED   10832746
REFERENCE   9  (bases 1 to 5848)
  AUTHORS   Arden,K.C., Viars,C.S., Fu,K. and Rozen,R.
  TITLE     Localization of short/branched chain acyl-CoA dehydrogenase
            (ACADSB) to human chromosome 10
  JOURNAL   Genomics 25 (3), 743-745 (1995)
   PUBMED   7759115
REFERENCE   10 (bases 1 to 5848)
  AUTHORS   Rozen,R., Vockley,J., Zhou,L., Milos,R., Willard,J., Fu,K.,
            Vicanek,C., Low-Nang,L., Torban,E. and Fournier,B.
  TITLE     Isolation and expression of a cDNA encoding the precursor for a
            novel member (ACADSB) of the acyl-CoA dehydrogenase gene family
  JOURNAL   Genomics 24 (2), 280-287 (1994)
   PUBMED   7698750
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA964130.1, BC013756.1,
            U12778.1 and AC012391.10.
            
            On Aug 31, 2019 this sequence version replaced NM_001609.3.
            
            Summary: Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a
            member of the acyl-CoA dehydrogenase family of enzymes that
            catalyze the dehydrogenation of acyl-CoA derivatives in the
            metabolism of fatty acids or branch chained amino acids. Substrate
            specificity is the primary characteristic used to define members of
            this gene family. The ACADSB gene product has the greatest activity
            towards the short branched chain acyl-CoA derivative,
            (S)-2-methylbutyryl-CoA, but also reacts significantly with other
            2-methyl branched chain substrates and with short straight chain
            acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein
            which is cleaved upon mitochondrial import and predicted to yield a
            mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: The 3' UTR extension represented by the RefSeq
            transcript record was derived from genomic sequence data to
            optimize consistency to the reference genome assembly. The extent
            of the UTR extension and the location of the polyA site was based
            on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL831821.2, SRR18074968.1994824.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000358776.7/
                                                  ENSP00000357873.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                DA964130.1         94-105
            13-61               BC013756.1         1-49
            62-374              U12778.1           53-365
            375-2399            BC013756.1         363-2387
            2400-5848           AC012391.10        7380-10828
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q26.13"
     gene            1..5848
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /note="acyl-CoA dehydrogenase short/branched chain"
                     /db_xref="GeneID:36"
                     /db_xref="HGNC:HGNC:91"
                     /db_xref="MIM:600301"
     exon            1..66
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1323
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /EC_number="1.3.8.5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; acyl-Coenzyme A dehydrogenase, short/branched
                     chain; 2-methyl branched chain acyl-CoA dehydrogenase;
                     short/branched chain specific acyl-CoA dehydrogenase,
                     mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase"
                     /codon_start=1
                     /product="short/branched chain specific acyl-CoA
                     dehydrogenase, mitochondrial isoform 1 precursor"
                     /protein_id="NP_001600.1"
                     /db_xref="CCDS:CCDS7634.1"
                     /db_xref="GeneID:36"
                     /db_xref="HGNC:HGNC:91"
                     /db_xref="MIM:600301"
                     /translation="MEGLAVRLLRGSRLLRRNFLTCLSSWKIPPHVSKSSQSEALLNI
                     TNNGIHFAPLQTFTDEEMMIKSSVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGL
                     MGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKA
                     TYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFL
                     VMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANIL
                     GQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQH
                     QVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGV
                     GYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY"
     exon            67..226
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            227..327
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            328..534
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            535..705
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            706..831
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            832..924
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1014
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1152
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1252
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..5848
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5826..5831
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /note="hexamer: AATAAA"
     polyA_site      5848
                     /gene="ACADSB"
                     /gene_synonym="2-MEBCAD; ACAD7; SBCAD"
                     /note="major polyA site"
ORIGIN      
        1 agagcggaga ggcctgcggc gaggatggag ggcctggcag tgcggttgct gcgcggcagc
       61 aggctgctaa gaagaaattt cctgacttgt ttgtcttctt ggaagattcc tcctcatgtc
      121 tcaaaatctt cccagtcaga agctctactc aatataacaa ataatggaat acactttgct
      181 cccctgcaaa catttacaga tgaggaaatg atgataaaga gttcagttaa aaaatttgct
      241 caggaacaaa ttgcaccttt ggtttcaacc atggatgaaa attcgaaaat ggagaaatca
      301 gtaatacaag gattatttca acaagggttg atgggtattg aagttgaccc agaatatgga
      361 ggcacaggag cttcattttt atccactgtg ctcgtgatag aggaattagc caaagttgat
      421 gcatctgtgg ctgtcttttg tgagatccag aacacattaa ttaacacact gattagaaaa
      481 catggaacag aagaacaaaa ggccacctat ttgcctcagc tcactacaga aaaagtagga
      541 agtttctgcc tttcagaggc tggagcaggt agtgactcat ttgctttgaa gaccagagct
      601 gataaagagg gagattatta tgtcctcaat ggatcaaaga tgtggatcag cagtgctgag
      661 cacgcagggc tctttctggt gatggcaaat gtagacccta ccattggata taagggaatt
      721 acctccttct tagtagatcg tgatactccg ggccttcata tagggaaacc tgaaaacaaa
      781 ttggggctca gagcttcttc cacctgcccg ttaacattcg aaaatgtcaa ggttccagaa
      841 gccaatatct tgggacaaat tggacatggc tataagtatg ccatagggag tctcaatgaa
      901 ggtagaatag gaattgctgc acagatgctg ggactggcgc aaggatgttt tgactacact
      961 attccatata ttaaagaaag gatacaattt ggcaaaagac tatttgattt tcagggcctc
     1021 caacaccaag tggctcacgt ggccacccag ctggaagctg caagattact aacatacaat
     1081 gctgctaggc ttttagaagc tggaaagcca ttcataaaag aagcgtcaat ggccaaatac
     1141 tatgcatcag agattgcagg acaaacaacg agtaaatgta tcgagtggat ggggggagta
     1201 ggctacacca aagattaccc tgtggagaaa tacttccgag atgcaaagat tggtacgata
     1261 tatgaaggag cttccaacat ccagttgaac accattgcaa agcatatcga tgcagaatac
     1321 tgacgtctat aggagtggga cccctccctg gtgtcactgc tgtaaaattt taaacggttg
     1381 tgtcttgttg ggagtaagtg ccttgcgtgg gaataaactt ccacagcatt cgaatatttt
     1441 aatgaagccc ttagtcaggg tcctggtgtt ggcctttttg gttttctctt ttcaggctgt
     1501 ttaacttagg cacaggagat ccacttttaa acttgggaaa taagcacctg tatttttttc
     1561 caaaactgtt tttaaagctg tatacgcata catatatata tttttactct gtcttactct
     1621 gtcacccagg ctagagtgca gtggcgcgat ctcagctcac tgcagccttg acctcctggg
     1681 ttccagtgat tctcatgcct catcctccca agtagctgga actacaggtg tgcaccacca
     1741 tgcctggttc atttttgtgt ttttagtaga gatggggttt taccatattg cccaggctgg
     1801 tcttctggct tctggatatc gcccaccttg gcctcccaaa gtgctgggat tacagggatg
     1861 agccaccgtg cctggctggg tatttatatt atcattctag tttcagagta tacagaagtt
     1921 tcatcccatc atttggaaaa ataaaggcat ctgaagtaca atattactta tagaaatagt
     1981 ttatattcct attaaatctt aatcttgtgg catcagggaa atatttgtta catagtaggc
     2041 aatttttatc cagtacttta tagattcaac tctaagttgc aagcgaagtc aaaactgatg
     2101 aaaatttatt tagaaaaatc taaaaattct ggttttaaat atgagaatca gtggaaaata
     2161 agggtataat tttgtaggtc atatgattga agaaaatatt attttaacaa tgtaaagcaa
     2221 tatgatttgt tactataatt aacctgtata aaagatacat tttatggtgg tttcagtagg
     2281 tcattttaaa aaccaatgtg cattagtttt caagtataag gtttaagtaa tttggtttaa
     2341 taatcagaaa atattcaata cagtgttgga tattctgtca tgcactattt ttcagttgac
     2401 aatttctgta ttttaattga atactgtttc ttcagtcatg gttattgcac tttatcctga
     2461 ataataattc agaaattggg ttttggttca gtgattctca agaaaaagat ctcttgccca
     2521 ttaagaagtg tatcaaaatc tcataaggaa tgagggagag aagggggctg tagagtttga
     2581 aaaagcatat tcaatattaa aatagtttta tatttgggaa ggcaaaaatg aatctattgt
     2641 tttgcaatat aggttataaa caggcaaaat gcaataaaat atatatctgg ttgtattagt
     2701 ctgttttcac gctgctgata aagacatacc tgagaccagg aagaaaaaga ggtttaattg
     2761 gacttacagt tccacatgac tggggaggcc tcagaatcat ggtgggaggc aaaaagcact
     2821 tcttacatgg cggcagcaag agaaaatgag aaagaagcaa aagtggaaac ccctaataaa
     2881 cctatcagat cttgtgagac ttactatcac gagaatagca caggaaagac tggcccccat
     2941 gattcaatta cctcccccgg gtccctccca caacacatgg gaattctgag agataccatt
     3001 taagttgaga tttcagtggg gacacagcca aaccatatca ttctacccct ggcacctcca
     3061 aatctcatgt cctcacattt caaaaccaat catgccttcc caacagtccc ccaaagtctt
     3121 aactcatttc agcattaacc caaaagtccg cagtccaaag tttcatctga gacaaggcaa
     3181 gtcccttcca cttatgaacc tgtaaaatca aaagtaaact agttacttcc tagatacaat
     3241 gggggtacag gtattgggta aatacagcca ttccaaatgg gagaaattgg ccaacacaaa
     3301 agggtaacag ggcccatgca agtccaaaat tcagcagggc agtcaaattt taaacctcca
     3361 aaatgatcac ctttgactcc acgtctcaca atcatctctc tcaagttcga agttccacaa
     3421 atctctaagg caagggaaag atgctgccag tctctttgct aaaacacagc aagagtcacc
     3481 tttactccag ttcccaaaaa gtttttcatc tccatctgag accacctcag cctgaatttt
     3541 attgtcaata ttgctatcag cattttgggc aaagccattc agcaagtatc taggaagttc
     3601 caaactttcc cacattttcc tgtcttcttc tgagcccttc aaactgttcc aacctctgcc
     3661 tgttacccag ttccaaactt gctttcatat ttttggctat cttttcagca atgccccact
     3721 ctactgttag cagtttactg tattagtctg ttttcacact gctgataaag acattcctca
     3781 agattgtgaa gaaaaagagg tttaattgga cttacagttc cacatggccg gggaggcctc
     3841 agaatcatgg cgggaggtga aaagcacttc gtacatggtg gtggcaagag aaaatgaaga
     3901 agaagcaaaa gtggaaaccc ctgataaacc catcagatct catgagactt actatcatga
     3961 gaatagcaca ggaaagactg gcccccctga ttcgatgacc tccccctggg tccctcccac
     4021 aacatgtggg aattccagaa gataaattca agttgcaatt tcagtgggga catagccaaa
     4081 ccatatcact ggtgatgcca cttcttcagt attagggatt ctcagtcaga agagaccccc
     4141 tgtgtggcct gagtcccctc aggaggaagg tggacaacag agaaatgaga gttttgatat
     4201 tttctgaaag aggaacatgt gttagagatg aagaatcttc caaggctcat gcagttgctt
     4261 agaataatca ttactgttat atgagaaaca ttttagtaat ttaataaaag gataatgttt
     4321 atttaaaaaa cctgactttt ccagagtaat tttgttttgc acattcatgt ttattgaagt
     4381 ggactaattt ctataatgca aatcagagtt aaatattaaa aattgtgtaa atacaattga
     4441 cataggaatt acattaaaat attaggaaga aacaaggaca aatttagacc ttgaatccga
     4501 agagataaag cttacttgac tttcaaatgg agagatgatg aaaacccact cattcactct
     4561 ttcagaacaa aaagacagtc atctgataag agtatgacat ggatgaaatg ccctacaggg
     4621 gccttggaca tctttaattt ctgcgattat gtgaaagagg tggactttac agataatgga
     4681 gcagaagcca acattagtaa aaggaatccc aacttcttcc catagaatta gaaacatgtg
     4741 aaagtacaat aaacttcttg ttcaaattac cagcatcaga gagcttccca tttgcatcta
     4801 gaccttgaat ttatatttat tgatcaagtt ctaatttgta tgtatatttt gtgcatattc
     4861 accaataaca gttaaaatta attatgtgtt atagttaata tatgcaccta ccttcttccg
     4921 ttagtgcatc agtaaatgtg ttattttgtc atttttccaa agagagtgtt gtaggttttc
     4981 cctgtagttc ttcctttata gcttttcttc tgataaccat gacttcagga gctttaaaac
     5041 tatctatctt gcatttgtgt ctggcggaga actagccatc agcctcctga agcctgccat
     5101 cattgttaat ttgaggactg ggctgtcttg gggctcagaa gataaagaac tatttgagca
     5161 gatgtgtgtg ggtggcactg gattccaccc aactgccaag ttagtattgt tagagatttc
     5221 attttacaac acaaaaataa gcctgtgtca aagattttaa aatcatggaa agttaaaatc
     5281 tagaaagacc ttagagaacc agccaaccaa ctctctcatt ttaaaagtga aggattcata
     5341 gcacagatta cttgcctaag atcatccagg aacgaagaca agaatccaaa tgtacttggg
     5401 gacaagaatt agtccccaaa ttcagtgttc ttcctagtat taaacattgc ccctttcgac
     5461 aaattttgga tttcattctt gttatatttc agtactcttg ctgatttatt ttaggtttaa
     5521 ctggtaaaga atgaacattt cagaagtgtt aggattagtc agtgtcatcg cttatgtata
     5581 tgaatcactt gccaaagaca ctggctctcc tcagctgtaa ccagcatgtc aaatgtgaag
     5641 attattgtgt gggatccttt ggtttcattg ttatgatgta ttttataaat gtactagtta
     5701 tatccttgtt tatgtatttt tgctgatctt tcataaagca gaatggtatg tatcggattg
     5761 ttttaatgtt atatattgga ttgtattcga tgttacaaaa ccaatattct atggagaatg
     5821 aaaaaaataa aggactaaat taactgga
//
