LOCUS       NM_022437               7180 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8),
            transcript variant 1, mRNA.
ACCESSION   NM_022437
VERSION     NM_022437.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Mi,J., Jiang,Q., Qi,Z., Liu,Z., Bai,X., Zheng,X., Wu,J., Fang,Y.,
            Yang,A. and Chen,H.
  TITLE     Plasma campesterol and ABCG5/ABCG8 gene loci on the risk of
            cholelithiasis and cholecystitis: evidence from Mendelian
            randomization and colocalization analyses
  JOURNAL   Hum Genomics 18 (1), 19 (2024)
   PUBMED   38347599
  REMARK    GeneRIF: Plasma campesterol and ABCG5/ABCG8 gene loci on the risk
            of cholelithiasis and cholecystitis: evidence from Mendelian
            randomization and colocalization analyses.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Farhat,D., Rezaei,F., Ristovski,M., Yang,Y., Stancescu,A.,
            Dzimkova,L., Samnani,S., Couture,J.F. and Lee,J.Y.
  TITLE     Structural Analysis of Cholesterol Binding and Sterol Selectivity
            by ABCG5/G8
  JOURNAL   J Mol Biol 434 (20), 167795 (2022)
   PUBMED   35988751
  REMARK    GeneRIF: Structural Analysis of Cholesterol Binding and Sterol
            Selectivity by ABCG5/G8.
REFERENCE   3  (bases 1 to 7180)
  AUTHORS   Krawczyk,M., Niewiadomska,O., Jankowska,I., Jankowski,K.,
            Wieckowski,S., Lebensztejn,D., Wiecek,S., Gozdowska,J., Kulaga,Z.,
            Weber,S.N., Lutjohann,D., Lammert,F. and Socha,P.
  TITLE     Common variant p.D19H of the hepatobiliary sterol transporter ABCG8
            increases the risk of gallstones in children
  JOURNAL   Liver Int 42 (7), 1585-1592 (2022)
   PUBMED   35129276
  REMARK    GeneRIF: Common variant p.D19H of the hepatobiliary sterol
            transporter ABCG8 increases the risk of gallstones in children.
REFERENCE   4  (bases 1 to 7180)
  AUTHORS   Tada,M.T., Rocha,V.Z., Lima,I.R., Oliveira,T.G.M., Chacra,A.P.,
            Miname,M.H., Nunes,V.S., Nakandakare,E.R., Costa Gurgel
            Castelo,M.H., Jannes,C.E., Santos,R.D., Krieger,J.E. and
            Pereira,A.C.
  TITLE     Screening of ABCG5 and ABCG8 Genes for Sitosterolemia in a Familial
            Hypercholesterolemia Cascade Screening Program
  JOURNAL   Circ Genom Precis Med 15 (3), e003390 (2022)
   PUBMED   35549507
  REMARK    GeneRIF: Screening of ABCG5 and ABCG8 Genes for Sitosterolemia in a
            Familial Hypercholesterolemia Cascade Screening Program.
REFERENCE   5  (bases 1 to 7180)
  AUTHORS   Berge,K.E., von Bergmann,K., Lutjohann,D., Guerra,R., Grundy,S.M.,
            Hobbs,H.H. and Cohen,J.C.
  TITLE     Heritability of plasma noncholesterol sterols and relationship to
            DNA sequence polymorphism in ABCG5 and ABCG8
  JOURNAL   J Lipid Res 43 (3), 486-494 (2002)
   PUBMED   11893785
  REMARK    GeneRIF: Common DNA sequence polymorphisms in the ABCG8 gene
            contribute to heritable variation in the plasma concentrations of
            the plant sterols campesterol and sitosterol.
REFERENCE   6  (bases 1 to 7180)
  AUTHORS   Hubacek,J.A., Berge,K.E., Cohen,J.C. and Hobbs,H.H.
  TITLE     Mutations in ATP-cassette binding proteins G5 (ABCG5) and G8
            (ABCG8) causing sitosterolemia
  JOURNAL   Hum Mutat 18 (4), 359-360 (2001)
   PUBMED   11668628
  REMARK    GeneRIF: mutations in ATP-binding cassette proteins G5 (ABCG5) and
            G8 (ABCG8) causing sitosterolemia
REFERENCE   7  (bases 1 to 7180)
  AUTHORS   Schmitz,G., Langmann,T. and Heimerl,S.
  TITLE     Role of ABCG1 and other ABCG family members in lipid metabolism
  JOURNAL   J Lipid Res 42 (10), 1513-1520 (2001)
   PUBMED   11590207
  REMARK    Review article
REFERENCE   8  (bases 1 to 7180)
  AUTHORS   Lu,K., Lee,M.H., Hazard,S., Brooks-Wilson,A., Hidaka,H., Kojima,H.,
            Ose,L., Stalenhoef,A.F., Mietinnen,T., Bjorkhem,I., Bruckert,E.,
            Pandya,A., Brewer,H.B. Jr., Salen,G., Dean,M., Srivastava,A. and
            Patel,S.B.
  TITLE     Two genes that map to the STSL locus cause sitosterolemia: genomic
            structure and spectrum of mutations involving sterolin-1 and
            sterolin-2, encoded by ABCG5 and ABCG8, respectively
  JOURNAL   Am J Hum Genet 69 (2), 278-290 (2001)
   PUBMED   11452359
REFERENCE   9  (bases 1 to 7180)
  AUTHORS   Berge,K.E., Tian,H., Graf,G.A., Yu,L., Grishin,N.V., Schultz,J.,
            Kwiterovich,P., Shan,B., Barnes,R. and Hobbs,H.H.
  TITLE     Accumulation of dietary cholesterol in sitosterolemia caused by
            mutations in adjacent ABC transporters
  JOURNAL   Science 290 (5497), 1771-1775 (2000)
   PUBMED   11099417
REFERENCE   10 (bases 1 to 7180)
  AUTHORS   Myrie,S.B., Steiner,R.D. and Mymin,D.
  TITLE     Sitosterolemia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   23556150
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Karyn Megy. The reference sequence was derived
            from AC108476.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 26, 2018 this sequence version replaced NM_022437.2.
            
            Summary: The protein encoded by this gene is a member of the
            superfamily of ATP-binding cassette (ABC) transporters. ABC
            proteins transport various molecules across extra- and
            intra-cellular membranes. ABC genes are divided into seven distinct
            subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This
            protein is a member of the White subfamily. The protein encoded by
            this gene functions to exclude non-cholesterol sterol entry at the
            intestinal level, promote excretion of cholesterol and sterols into
            bile, and to facilitate transport of sterols back into the
            intestinal lumen. It is expressed in a tissue-specific manner in
            the liver, intestine, and gallbladder. This gene is tandemly
            arrayed on chromosome 2, in a head-to-head orientation with family
            member ABCG5. Mutations in this gene may contribute to sterol
            accumulation and atherosclerosis, and have been observed in
            patients with sitosterolemia. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF324494.1, BC113657.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144835, SAMEA2145122
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272286.4/ ENSP00000272286.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-146               AC108476.5         19157-19302
            147-248             AC108476.5         24693-24794
            249-405             AC108476.5         26341-26497
            406-644             AC108476.5         31770-32008
            645-777             AC108476.5         32540-32672
            778-1047            AC108476.5         32785-33054
            1048-1210           AC108476.5         52162-52324
            1211-1294           AC108476.5         52409-52492
            1295-1494           AC108476.5         53973-54172
            1495-1571           AC108476.5         54593-54669
            1572-1839           AC108476.5         55332-55599
            1840-1967           AC108476.5         57747-57874
            1968-7180           AC108476.5         57962-63174
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p21"
     gene            1..7180
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="ATP binding cassette subfamily G member 8"
                     /db_xref="GeneID:64241"
                     /db_xref="HGNC:HGNC:13887"
                     /db_xref="MIM:605460"
     exon            1..146
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..2105
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="ATP-binding cassette, subfamily G, member 8;
                     isoform 1 is encoded by transcript variant 1; sterolin 2;
                     ATP-binding cassette, sub-family G (WHITE), member 8"
                     /codon_start=1
                     /product="ATP-binding cassette sub-family G member 8
                     isoform 1"
                     /protein_id="NP_071882.1"
                     /db_xref="CCDS:CCDS1815.1"
                     /db_xref="GeneID:64241"
                     /db_xref="HGNC:HGNC:13887"
                     /db_xref="MIM:605460"
                     /translation="MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQP
                     NTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLA
                     IIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPN
                     LTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGER
                     RRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDI
                     FRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQ
                     ELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKM
                     PGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAA
                     LLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYI
                     IIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNAL
                     YNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAV
                     SGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW"
     exon            147..248
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            249..405
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            406..644
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            645..777
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            778..1047
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1210
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1294
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1494
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1571
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1839
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1967
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..7180
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2636..2641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="hexamer: ATTAAA"
     polyA_site      2658
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="major polyA site"
     regulatory      6298..6303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="hexamer: ATTAAA"
     polyA_site      6320
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="major polyA site"
     regulatory      6706..6711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="hexamer: AATATA"
     polyA_site      6724
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
     regulatory      7159..7164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
                     /note="hexamer: AATAAA"
     polyA_site      7180
                     /gene="ABCG8"
                     /gene_synonym="GBD4; STSL; STSL1"
ORIGIN      
        1 aaacagagtg aagacactgg ccctggcagg cagcagctgg gtctaagaga gctgcagccc
       61 agggtcacag acctgtgggc cccatggccg ggaaggcggc agaggagaga gggctgccga
      121 aaggggccac tccccaggat acctcgggcc tccaggatag attgttctcc tctgaaagtg
      181 acaacagcct gtacttcacc tacagtggcc agcccaacac cctggaggtc agagacctca
      241 actaccaggt ggacctggcc tctcaggtcc cttggtttga gcagctggct cagttcaaga
      301 tgccctggac atctcccagc tgccagaatt cttgtgagct gggcatccag aacctaagct
      361 tcaaagtgag aagtgggcag atgctggcca tcatagggag ctcaggttgt gggagagcct
      421 ccttgctaga tgtgatcact ggccgaggtc acggcggcaa gatcaagtca ggccagatct
      481 ggatcaatgg gcagcccagc tcgcctcagc tggtgaggaa gtgtgtggcc cacgtgcgcc
      541 agcacaacca gctgctcccc aacttgactg tgcgagagac cttggccttc attgcccaga
      601 tgcggctgcc cagaaccttc tcccaggccc agcgtgacaa aagggtggag gacgtgatcg
      661 cggagctgcg gcttaggcag tgcgctgaca cccgcgtggg caacatgtac gtgcgggggt
      721 tgtcgggggg tgagcgcagg agagtcagca ttggggtgca gctcctgtgg aacccaggaa
      781 tccttattct cgacgaaccc acctctgggc tcgacagctt cacagcccac aacctggtga
      841 agaccttgtc caggctggcc aaaggcaacc ggctggtgct catctccctc caccagcctc
      901 gctctgacat cttcaggctg tttgatctgg tcctcctgat gacgtctggc acccccatct
      961 acttaggggc ggcccagcac atggtccagt atttcacagc catcggctac ccctgtcctc
     1021 gctacagcaa tcctgctgac ttctatgtgg acctgaccag cattgacagg cgcagcagag
     1081 agcaggaatt ggccaccagg gagaaggctc agtcactcgc agccctgttt ctagaaaaag
     1141 tgcgtgactt agatgacttt ctatggaaag cagagacgaa ggatcttgac gaggacacct
     1201 gtgtggaaag cagcgtgacc ccactagaca ccaactgcct cccgagtcct acgaagatgc
     1261 ctggggcggt gcagcagttt acgacgctga tccgtcgtca gatttccaac gacttccgag
     1321 acctgcccac cctcctcatc catggggcgg aggcctgtct gatgtcaatg accatcggct
     1381 tcctctattt tggccatggg agcatccagc tctccttcat ggatacagcc gccctcttgt
     1441 tcatgatcgg tgctctcatc cctttcaacg tcattctgga tgtcatctcc aaatgttact
     1501 cagagagggc aatgctttac tatgaactgg aagacgggct gtacaccact ggtccatatt
     1561 tctttgccaa gatcctcggg gagcttccgg agcactgtgc ctacatcatc atctacggga
     1621 tgcccaccta ctggctggcc aacctgaggc caggcctcca gcccttcctg ctgcacttcc
     1681 tgctggtgtg gctggtggtc ttctgttgca ggattatggc cctggccgcc gcggccctgc
     1741 tccccacctt ccacatggcc tccttcttca gcaatgccct ctacaactcc ttctacctcg
     1801 ccgggggctt catgataaac ttgagcagcc tgtggacagt gcccgcgtgg atttccaaag
     1861 tgtccttcct gcggtggtgt tttgaagggc tgatgaagat tcagttcagc agaagaactt
     1921 ataaaatgcc tctcgggaac ctcaccatcg cggtctcagg agataaaatc ctcagtgtca
     1981 tggagctgga ctcgtaccct ctctacgcca tctacctcat cgtcattggc ctcagcggtg
     2041 gcttcatggt cctgtactac gtgtccttaa ggttcatcaa acagaaacca agtcaagact
     2101 ggtgattcac gccagacgtc tgcccgctgg tgggggacct gagcagaccc ttcaactgca
     2161 ctccctcctc aggagcccct tcctggggac agtgaggaca atgaccctac agatgctcag
     2221 ctacatccgg cccagggtgc tgcagtggca cagaccagcc acaggatggc agtagaataa
     2281 agacagtcga aagggatttc tgctcactgg caggagactg cgatgactgg gagaaaacct
     2341 gcactcggtg gcacctacaa cgttgctaat ttatttcctt ttgatatgca tttatatagg
     2401 caactcgata taggatggga gcaaactagg aatgaattgg gtagctagac tgtgcaggaa
     2461 ttgttggaac ctggagggaa caataacagt agctagcaga tttggcttca tcttccaggg
     2521 gccccacact ccgtggtgag ccaccatcaa tacagaaagt gacctaagat gtaccagcaa
     2581 gatgccatcc cttctttttg tgtggggtca tgggctccaa aagccaacgt gaacaattaa
     2641 aaatgtattg agcatctact ctgtagcagg tcctgtgaaa acactttagg tggacaatcc
     2701 cttgaggtaa gtggtatccc attttatagg tgtgaaaact gaagcaaaaa ttcattttcc
     2761 taagggcaca tggatacttt gtggtggagt catatgggga tcagaaaagc ctttgaggcc
     2821 tttggagtta gagggcagaa ggcaaggcct gagccgctgt aagccttagg agtttaggaa
     2881 ggctccagaa gacaaatggg gtctgtagag gctgttaact cagccaggct tcttagagtt
     2941 gcatttcact aactgatatg gtttggctct gcgtcctcac ccaaatctca ccttgaattg
     3001 taataatccc caagtgtcaa gggcgggacc agatggagat aattgaatca taggggtggt
     3061 ttctctgatg ctgttctcct gagagtgagt gagttctgat gagatccgac ggttttataa
     3121 ggggcttccc tcttcgctcg gctctcattc tctctcccgc taccctgtga agaggagcct
     3181 tccaccacga ctgcaagttt cctgaggctg ccccagccgt gctgaactgt gagtcagtta
     3241 aacctctttt ctttataaat tacccagtct tgggtatttc ttcatagcag tgtgagagca
     3301 gatgaataca ctggccctgc ctgggtttca gaaccagcct tgaacctttc acagtggcca
     3361 gaggatgggg aggcagaggc ccaggttgca cacttcttgc ctgagtgttg gggactatct
     3421 gacccaaaac aggtgcacag agggcaggag aggatgttcc caaaggaaaa ttagagttta
     3481 gaatcaaaag aaggggaagg tgcgtgtttg ggaggtaaat agcaaatact cttcataggt
     3541 tcactagagt cttgctactc caagtacgat ccctgggcca gcagaatggg cacagctgga
     3601 gctgattgga taggtcccat gagcctcagg ccccacccag gctcaatgag tcagagtctg
     3661 cgtcttaaga agaccccctg gtgatctgtg cacattcaag catgctgccg ttttccaaag
     3721 cacttgcaac actcaggatg cttgcacggt catgttgcca ccatccaacc tgcagacccc
     3781 attcttgaga ttgactggga gttcctatca tgtcctccat agcaagggga tctagaccag
     3841 aatcaagcct tggatctagt tctcaagtct cttttgtctc tttcagttta ggaacagttt
     3901 gtcaactttc cttcactttt gtgaccttga tacttgagtt tgaaggctgt ctctcaattt
     3961 gtgttctgcc cagtgcatcc tatcagaagg catgtgattt caacttctcc cataccaaca
     4021 acgttcactt tgatcacttg attaaagggg tgtctgctag gcttctccac agccaagtta
     4081 ctattttccc tccctttata attaataagc attttgtaag tgggtacttt gaaactatgt
     4141 aaattgtaaa ctttccattt atgcatttta aaattttgat tgatgtaaac ttgtgaattc
     4201 ccattgtatc cttacaaccc tttattattg tgattaattc tgatgctcag cttgtccctg
     4261 atttggccag tgggaacccc gtcgagctgg cttctgcatc cttttgatat gtcctcatca
     4321 tcctttgagg gctgaaacaa ccaagagttt caggctcatt ttttgttttg tttttttttt
     4381 tttgagacag aatctcattc tgtggcccag gctggagtgc aatggcgcga tctcggctca
     4441 ctgcaacctc tgcctcccag gttcaagcga ttgccttgcc tcagcctccc aagtagttgg
     4501 gattaacagt gcccaccacc acacctggct aatttttgta tttttagtaa agacagtgtt
     4561 tcaccatatt ggccagactg gttgtgaact ctaggcctca agtgatctgc ctgcttcggc
     4621 ctcccaaagt gttgggatta caggtgtgac ccaccgcacc tggtgtcaca ggttcatctt
     4681 gtattttccc atctcagccc tggaatccga tgtttctcca gagaatctgg gttcctctta
     4741 gtggaaaatg gtatttagaa gccaaggttt tggtgataag tatgctcttt gcttgtggaa
     4801 catctctgct cccaggctct ctcagtgaac agagttaggg agtgtgtgtg tgtgtgcgcg
     4861 cgcgcgcgcg catgtgcata catatacacc taagtacaca tgagtttact ctgataacat
     4921 ccatttccaa tccagcatca cagagttgat cctaggttct tccctcttct ctaattgtga
     4981 gaaacctggc ttccattacc cttaggttat ttactcattt gattaaccgc ctgcaggtaa
     5041 gtaatctccc atctacactg ccacgccttc tcctgtatgg acgtcctgcc taccctgctc
     5101 aggctctaac tcccaagtcc agctttccct gagtctgcag ctgtcttcct cacactcccg
     5161 tggcccacca caacttcccc ccacctcctt gtcactgtgg tcccctaatg gcgttaggcc
     5221 tgaatgaaga agggggaagg atgaagagga cattggtagc ttgccttaac tccccaggta
     5281 attctaatgt gcagtgattg acagccacta agatacatca cctgactttg tgagttcacc
     5341 attcggtctg caatgggttt tggtaactca gtggctctgt atgccgtgct gaaggggaaa
     5401 agggcaaagt gcaaaggcag gagggatgga gaaaatatag cagctgccat ttatttgtgt
     5461 ttttcctaca gcggtggttc tccaccctgg ttgcccatgc tggagcttta acaaaagcca
     5521 atgtggaccc attgcccaga gactctgatc ccatgggtct gttttgttta aaatgctctt
     5581 caaggcattg tgaggccagg aaaacaaaga accaaggctc tcccgggtgc ggtggctcac
     5641 gcctgtaatc ccagcacttt gggaggccgc ggagggcaga tcacgaggtc aggagatcga
     5701 gaccattctg gccaacatgg caaaaccctg cctctactaa aaatacaaaa attagctggg
     5761 catggtagtg cgtgcctgta gttccagcta cttgggaggt tgaggcagga gaatcgcttg
     5821 aatccgggag gcagaggttg cagtgagccg agattgagcc accgcactcc agcctggtga
     5881 cagagcgata ctctgtctca aaaaaaaaaa aaaaaaaccc aaggctctcg aggcatgcat
     5941 gctgttcagc gagccctgcc ctgcattgcg aaagggtaga gatttgttta tttgtcagta
     6001 ttgaaagcat ggcttgcaaa gttttcctgt taagagtcag atagtaaata tttgggactt
     6061 cgtgggcctt ataggctctg ctgcaattac ttaactctgc tgttacagtg tgagagcagc
     6121 tgtagactat agtaacgtac agccggtgtg gctgtcttct aagaaagtat tatttacaaa
     6181 aacaggcagg ttgctagatt tgacctgagg gtgtagtttg cagagccttg gtaaaggttt
     6241 tcaaatttgc attttaagcc ctccaatttc aagttatgct attggtgggg aattttaatt
     6301 aaacagaaaa taatcaccaa tattggctca aatatttcct gatttaaaaa aaaatctgct
     6361 cttgttccta taaatagtat ataagatgta atcttgccct gggttaaacc agtctacaga
     6421 ggtagatctc gcatttgctt ttgttttctt ctacttcaac tcttgtcatg atcccaaatt
     6481 aaacaactag caacagaaat gttcaaatct gtcaaatcca agtgttaaca gacctgggac
     6541 caggggttcc tcatagtggc ttagcacagc atacagacca ctttcctttg gcccaaccgg
     6601 ccttggggtc tggagcacca aacaggccct gaggaagcgt gcaaggaact ggaatgcggg
     6661 gaaagtgatg aaatattttg ttggttggcc tttgcttgtt tgcaaaatat atccaatgct
     6721 cacacctgag agacaacaga catccttatt tgtgaatagt ctgcactaga taattcagat
     6781 ttcaagtcat ctgtcattcg tacttgataa tgatttgttc ttcatttaaa gtggcctggg
     6841 tacagagcaa catcccctgt ctttatcaca tgagctctgc ctgaggcagc ttcagcaggt
     6901 aggcaggcag gcagatggag cacagcccat tctctgctgg tgcactagga acagcagcac
     6961 gtgtggtgtt agcagctaat aattaaaaca acgtttaagc agtgggttga gatttttaaa
     7021 cacaaaccaa ctcaaaaaca cttctctgct ttctatgtaa tagtgctcac atcatttgta
     7081 tgattgtctc gtctctttaa aattggaaat attcttgttt ccccaaactg ctaataagct
     7141 ctactgaccc atgctaaaaa taaagctctt tcggctgggc
//
