LOCUS       NM_020297               8668 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens ATP binding cassette subfamily C member 9 (ABCC9),
            transcript variant SUR2B, mRNA.
ACCESSION   NM_020297 XM_005253284
VERSION     NM_020297.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Efthymiou,S., Scala,M., Nagaraj,V., Ochenkowska,K., Komdeur,F.L.,
            Liang,R.A., Abdel-Hamid,M.S., Sultan,T., Baroy,T., Van Ghelue,M.,
            Vona,B., Maroofian,R., Zafar,F., Alkuraya,F.S., Zaki,M.S.,
            Severino,M., Duru,K.C., Tryon,R.C., Brauteset,L.V., Ansari,M.,
            Hamilton,M., van Haelst,M.M., van Haaften,G., Zara,F., Houlden,H.,
            Samarut,E., Nichols,C.G., Smeland,M.F. and McClenaghan,C.
  TITLE     Novel loss-of-function variants expand ABCC9-related intellectual
            disability and myopathy syndrome
  JOURNAL   Brain 147 (5), 1822-1836 (2024)
   PUBMED   38217872
  REMARK    GeneRIF: Novel loss-of-function variants expand ABCC9-related
            intellectual disability and myopathy syndrome.
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   Sung,M.W., Yang,Z., Driggers,C.M., Patton,B.L., Mostofian,B.,
            Russo,J.D., Zuckerman,D.M. and Shyng,S.L.
  TITLE     Vascular KATP channel structural dynamics reveal regulatory
            mechanism by Mg-nucleotides
  JOURNAL   Proc Natl Acad Sci U S A 118 (44) (2021)
   PUBMED   34711681
  REMARK    GeneRIF: Vascular KATP channel structural dynamics reveal
            regulatory mechanism by Mg-nucleotides.
REFERENCE   3  (bases 1 to 8668)
  AUTHORS   Scala,R., Maqoud,F., Zizzo,N., Passantino,G., Mele,A.,
            Camerino,G.M., McClenaghan,C., Harter,T.M., Nichols,C.G. and
            Tricarico,D.
  TITLE     Consequences of SUR2[A478V] Mutation in Skeletal Muscle of Murine
            Model of Cantu Syndrome
  JOURNAL   Cells 10 (7), 1791 (2021)
   PUBMED   34359961
  REMARK    GeneRIF: Consequences of SUR2[A478V] Mutation in Skeletal Muscle of
            Murine Model of Cantu Syndrome.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8668)
  AUTHORS   Bienengraeber,M., Olson,T.M., Selivanov,V.A., Kathmann,E.C.,
            O'Cochlain,F., Gao,F., Karger,A.B., Ballew,J.D., Hodgson,D.M.,
            Zingman,L.V., Pang,Y.P., Alekseev,A.E. and Terzic,A.
  TITLE     ABCC9 mutations identified in human dilated cardiomyopathy disrupt
            catalytic KATP channel gating
  JOURNAL   Nat Genet 36 (4), 382-387 (2004)
   PUBMED   15034580
  REMARK    GeneRIF: role of mutation in human dilated cardiomyopathy and
            effect on KATP channel gating
REFERENCE   5  (bases 1 to 8668)
  AUTHORS   Davis-Taber,R., Choi,W., Feng,J., Hoogenboom,L., McNally,T.,
            Kroeger,P., Shieh,C.C., Simmer,R., Brioni,J.D., Sullivan,J.P.,
            Gopalakrishnan,M. and Scott,V.E.
  TITLE     Molecular characterization of human SUR2-containing K(ATP) channels
  JOURNAL   Gene 256 (1-2), 261-270 (2000)
   PUBMED   11054556
REFERENCE   6  (bases 1 to 8668)
  AUTHORS   Shindo,T., Yamada,M., Isomoto,S., Horio,Y. and Kurachi,Y.
  TITLE     SUR2 subtype (A and B)-dependent differential activation of the
            cloned ATP-sensitive K+ channels by pinacidil and nicorandil
  JOURNAL   Br J Pharmacol 124 (5), 985-991 (1998)
   PUBMED   9692785
REFERENCE   7  (bases 1 to 8668)
  AUTHORS   Aguilar-Bryan,L., Clement,J.P. 4th, Gonzalez,G., Kunjilwar,K.,
            Babenko,A. and Bryan,J.
  TITLE     Toward understanding the assembly and structure of KATP channels
  JOURNAL   Physiol Rev 78 (1), 227-245 (1998)
   PUBMED   9457174
  REMARK    Review article
REFERENCE   8  (bases 1 to 8668)
  AUTHORS   Grange,D.K., Nichols,C.G. and Singh,G.K.
  TITLE     Cantu Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   25275207
REFERENCE   9  (bases 1 to 8668)
  AUTHORS   Brugada,R., Campuzano,O., Sarquella-Brugada,G., Brugada,P.,
            Brugada,J. and Hong,K.
  TITLE     Brugada Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301690
REFERENCE   10 (bases 1 to 8668)
  AUTHORS   Hershberger,R.E. and Jordan,E.
  TITLE     Dilated Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301486
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC084806.14, AC008250.23,
            KF570253.1, KF459680.1 and KF495813.1.
            
            On or before Jun 22, 2020 this sequence version replaced
            XM_005253284.4, NM_020297.3.
            
            Summary: The protein encoded by this gene is a member of the
            superfamily of ATP-binding cassette (ABC) transporters. ABC
            proteins transport various molecules across extra- and
            intra-cellular membranes. ABC genes are divided into seven distinct
            subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This
            protein is a member of the MRP subfamily which is involved in
            multi-drug resistance. This protein is thought to form
            ATP-sensitive potassium channels in cardiac, skeletal, and vascular
            and non-vascular smooth muscle. Protein structure suggests a role
            as the drug-binding channel-modulating subunit of the
            extra-pancreatic ATP-sensitive potassium channels. Mutations in
            this gene are associated with cardiomyopathy dilated type 1O.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Apr 2011].
            
            Transcript Variant: This variant (SUR2B) uses an alternate 3'
            coding exon (exon 38B), compared to variant SUR2A, which uses exon
            38A. The encoded isoform (SUR2B) has an alternate 38-amino acid
            C-terminus, but is the same length as isoform SUR2A. There are no
            full-length transcripts representing this variant in human; it is
            supported by partial transcript alignments, by full-length
            transcript alignments from the homologous mouse and rat genes, and
            by RT-PCR analysis in PMID:11054556.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.6402.1,
                                           SRR14372079.3929771.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261200.9/ ENSP00000261200.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AC084806.14        41846-42072
            228-343             AC084806.14        42447-42562
            344-505             AC084806.14        46578-46739
            506-647             AC084806.14        49349-49490
            648-769             AC084806.14        57209-57330
            770-936             AC008250.23        140509-140675       c
            937-1179            AC008250.23        139240-139482       c
            1180-1374           AC008250.23        136444-136638       c
            1375-1527           KF570253.1         223-375             c
            1528-1683           KF459680.1         179-334             c
            1684-1818           AC008250.23        131649-131783       c
            1819-1981           AC008250.23        129698-129860       c
            1982-2022           AC008250.23        118847-118887       c
            2023-2165           AC008250.23        117604-117746       c
            2166-2274           AC008250.23        111398-111506       c
            2275-2382           AC008250.23        106338-106445       c
            2383-2455           AC008250.23        99226-99298         c
            2456-2561           AC008250.23        96197-96302         c
            2562-2600           KF495813.1         230-268
            2601-2702           AC008250.23        86525-86626         c
            2703-2787           AC008250.23        84543-84627         c
            2788-2868           AC008250.23        83158-83238         c
            2869-3006           AC008250.23        75940-76077         c
            3007-3132           AC008250.23        75669-75794         c
            3133-3229           AC008250.23        71722-71818         c
            3230-3459           AC008250.23        69175-69404         c
            3460-3608           AC008250.23        68339-68487         c
            3609-3678           AC008250.23        68055-68124         c
            3679-3836           AC008250.23        65886-66043         c
            3837-3929           AC008250.23        61650-61742         c
            3930-4032           AC008250.23        52530-52632         c
            4033-4134           AC008250.23        41722-41823         c
            4135-4255           AC008250.23        40759-40879         c
            4256-4386           AC008250.23        39335-39465         c
            4387-4465           AC008250.23        38216-38294         c
            4466-4574           AC008250.23        35621-35729         c
            4575-4678           AC008250.23        33424-33527         c
            4679-4812           AC008250.23        30918-31051         c
            4813-4875           AC008250.23        29570-29632         c
            4876-8668           AC008250.23        20961-24753         c
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..8668
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /note="ATP binding cassette subfamily C member 9"
                     /db_xref="GeneID:10060"
                     /db_xref="HGNC:HGNC:60"
                     /db_xref="MIM:601439"
     exon            1..227
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            228..343
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            344..505
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     CDS             364..5013
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /note="isoform SUR2B is encoded by transcript variant
                     SUR2B; ATP-binding cassette transporter sub-family C
                     member 9; sulfonylurea receptor 2; ATP-binding cassette,
                     sub-family C (CFTR/MRP), member 9"
                     /codon_start=1
                     /product="ATP-binding cassette sub-family C member 9
                     isoform SUR2B"
                     /protein_id="NP_064693.2"
                     /db_xref="CCDS:CCDS8693.1"
                     /db_xref="GeneID:10060"
                     /db_xref="HGNC:HGNC:60"
                     /db_xref="MIM:601439"
                     /translation="MSLSFCGNNISSYNINDGVLQNSCFVDALNLVPHVFLLFITFPI
                     LFIGWGSQSSKVQIHHNTWLHFPGHNLRWILTFALLFVHVCEIAEGIVSDSRRESRHL
                     HLFMPAVMGFVATTTSIVYYHNIETSNFPKLLLALFLYWVMAFITKTIKLVKYCQSGL
                     DISNLRFCITGMMVILNGLLMAVEINVIRVRRYVFFMNPQKVKPPEDLQDLGVRFLQP
                     FVNLLSKATYWWMNTLIISAHKKPIDLKAIGKLPIAMRAVTNYVCLKDAYEEQKKKVA
                     DHPNRTPSIWLAMYRAFGRPILLSSTFRYLADLLGFAGPLCISGIVQRVNETQNGTNN
                     TTGISETLSSKEFLENAYVLAVLLFLALILQRTFLQASYYVTIETGINLRGALLAMIY
                     NKILRLSTSNLSMGEMTLGQINNLVAIETNQLMWFLFLCPNLWAMPVQIIMGVILLYN
                     LLGSSALVGAAVIVLLAPIQYFIATKLAEAQKSTLDYSTERLKKTNEILKGIKLLKLY
                     AWEHIFCKSVEETRMKELSSLKTFALYTSLSIFMNAAIPIAAVLATFVTHAYASGNNL
                     KPAEAFASLSLFHILVTPLFLLSTVVRFAVKAIISVQKLNEFLLSDEIGDDSWRTGES
                     SLPFESCKKHTGVQPKTINRKQPGRYHLDSYEQSTRRLRPAETEDIAIKVTNGYFSWG
                     SGLATLSNIDIRIPTGQLTMIVGQVGCGKSSLLLAILGEMQTLEGKVHWSNVNESEPS
                     FEATRSRNRYSVAYAAQKPWLLNATVEENITFGSPFNKQRYKAVTDACSLQPDIDLLP
                     FGDQTEIGERGINLSGGQRQRICVARALYQNTNIVFLDDPFSALDIHLSDHLMQEGIL
                     KFLQDDKRTLVLVTHKLQYLTHADWIIAMKDGSVLREGTLKDIQTKDVELYEHWKTLM
                     NRQDQELEKDMEADQTTLERKTLRRAMYSREAKAQMEDEDEEEEEEEDEDDNMSTVMR
                     LRTKMPWKTCWRYLTSGGFFLLILMIFSKLLKHSVIVAIDYWLATWTSEYSINNTGKA
                     DQTYYVAGFSILCGAGIFLCLVTSLTVEWMGLTAAKNLHHNLLNKIILGPIRFFDTTP
                     LGLILNRFSADTNIIDQHIPPTLESLTRSTLLCLSAIGMISYATPVFLVALLPLGVAF
                     YFIQKYFRVASKDLQELDDSTQLPLLCHFSETAEGLTTIRAFRHETRFKQRMLELTDT
                     NNIAYLFLSAANRWLEVRTDYLGACIVLTASIASISGSSNSGLVGLGLLYALTITNYL
                     NWVVRNLADLEVQMGAVKKVNSFLTMESENYEGTMDPSQVPEHWPQEGEIKIHDLCVR
                     YENNLKPVLKHVKAYIKPGQKVGICGRTGSGKSSLSLAFFRMVDIFDGKIVIDGIDIS
                     KLPLHTLRSRLSIILQDPILFSGSIRFNLDPECKCTDDRLWEALEIAQLKNMVKSLPG
                     GLDAVVTEGGENFSVGQRQLFCLARAFVRKSSILIMDEATASIDMATENILQKVVMTA
                     FADRTVVTIAHRVHTILTADLVIVMKRGNILEYDTPESLLAQENGVFASFVRADM"
     exon            506..647
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            648..769
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            770..936
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1179
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1374
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1527
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1683
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1818
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1981
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2022
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2165
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2274
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2382
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2455
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2561
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2600
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2702
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2787
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2868
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..3006
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3007..3132
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3133..3229
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3459
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3608
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..3678
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3679..3836
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3837..3929
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            3930..4032
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4033..4134
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4135..4255
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4256..4386
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4387..4465
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4466..4574
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4575..4678
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4679..4812
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4813..4875
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     exon            4876..8668
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8642..8647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /note="hexamer: ATTAAA"
     polyA_site      8668
                     /gene="ABCC9"
                     /gene_synonym="ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2"
                     /note="major polyA site"
ORIGIN      
        1 gtcaaagctg agcagagcag ggatggggaa ggtctccggc cccaggcgcg gcgcgcgggg
       61 cttctgccca gtttcctgct tctcagccgc ggtgtctgcc ccggcccaga gcagtctgtg
      121 caatttagaa actcgatagg aggcagcagc tggtctccca ccaccctaaa aataatccgt
      181 tccggcgcac tgcgtgcttc gcctagggga ggaaaactgt catcggagag ttctgcgtcc
      241 gggtttgaaa tttacatctt aagacagtgt aggaagtcgg tgttttgaag gtagctcaag
      301 tgcaccggca ggggtttgaa gcagcgtgaa gctattgccc aagagtaaac catataagaa
      361 gaaatgagcc tttcattttg tggtaacaac atttcttcat ataatatcaa cgatggtgta
      421 ctacaaaatt cctgctttgt ggatgccctc aacctggtcc ctcatgtctt tctgttgttt
      481 atcacttttc caatattgtt tattgggtgg gggagccaaa gctcaaaagt acaaattcac
      541 cacaacacat ggcttcattt tccgggacat aacctgagat ggattcttac attcgctctc
      601 ctgtttgtgc atgtctgtga aatagcagaa ggcattgttt cagactcgcg gcgggaatca
      661 aggcacctcc acctctttat gccagccgtg atgggattcg ttgccactac aacatcgata
      721 gtgtattatc ataatatcga aacatcaaat tttcctaaat tacttttagc cctgttcctg
      781 tattgggtaa tggcctttat tacaaaaaca ataaaattgg ttaagtactg tcagtctggc
      841 ttggacatat caaacctgcg tttctgcatc acaggcatga tggtcatctt gaatgggctc
      901 ttgatggctg tggagatcaa tgtcattcga gtcaggagat atgtattttt catgaatcct
      961 cagaaagtaa agcctcctga agacctccag gatctgggag tgagatttct tcaaccattt
     1021 gtgaatttgc tgtcaaaagc aacatactgg tggatgaaca cacttattat atctgctcac
     1081 aaaaagccta ttgatctgaa ggcaattgga aaattgccaa tagcaatgag agcagtaaca
     1141 aattatgttt gcctgaaaga tgcatatgaa gaacaaaaga aaaaagttgc agatcatcca
     1201 aatcggactc catctatatg gcttgcaatg tacagagctt ttgggcgacc aattctactt
     1261 agtagcacat tccgctatct ggctgattta ctgggttttg ctggacctct ttgtatttct
     1321 ggaatagttc agcgtgtgaa tgaaacccag aatgggacaa ataacacaac tggaatttca
     1381 gaaaccctct catcaaagga atttcttgaa aacgcttacg ttctagcagt tcttctcttc
     1441 ttggctctta ttctgcaaag gacatttttg caggcttcct actatgtaac catagagact
     1501 ggcattaacc tccgtggagc tctgctggcc atgatttata ataaaatcct taggctctct
     1561 acgtctaact tatccatggg ggagatgact ctggggcaga tcaacaactt agtcgccatt
     1621 gaaactaatc aactcatgtg gtttttgttc ctgtgtccca atctatgggc tatgcctgtt
     1681 cagatcataa tgggcgtgat tctgctctat aatttacttg gatcaagtgc attggtcggt
     1741 gcagctgtca ttgtgctcct tgcgccaatt cagtacttta ttgctacaaa gttggcagag
     1801 gctcagaaaa gtacacttga ttattccact gagagactca agaaaacaaa tgaaatattg
     1861 aaaggcatca aacttctaaa attgtatgcc tgggaacaca ttttctgcaa aagtgtggag
     1921 gaaacaagaa tgaaagaact atctagtctc aaaacctttg cactatatac atcactctcc
     1981 atcttcatga atgcagcaat tcccatagca gctgttcttg ctacatttgt gacccatgcg
     2041 tatgccagtg gaaacaatct gaaacctgca gaggcctttg cttcactgtc tctcttccat
     2101 atcctggtca caccactgtt cctgctctcc acggtggtca gatttgcagt caaagccatc
     2161 ataagtgttc aaaagctgaa tgagtttctc ttgagtgatg agattggtga cgacagttgg
     2221 cgaactggtg aaagttcgct tccttttgag tcctgtaaga agcacactgg agttcagcca
     2281 aaaactataa acaggaaaca gcctggaaga tatcacctgg acagctatga gcaatcaaca
     2341 cggcgtctac gtcccgcaga aacagaggac attgcaataa aggtcacaaa tggatacttt
     2401 tcatggggca gtggtttagc tacattatcc aatatagata ttcgaattcc aacaggtcag
     2461 ttaaccatga ttgtgggcca agtaggatgt gggaagtcct ctcttctcct tgccatcctc
     2521 ggtgagatgc agacattgga aggaaaagtt cactggagca atgtaaatga atctgagcct
     2581 tcttttgaag caaccagaag taggaacagg tactctgtgg catatgcagc tcaaaagcct
     2641 tggctattaa atgctacagt agaagaaaat attacttttg gaagtccttt taacaaacag
     2701 aggtacaaag ctgtcacaga tgcctgttct cttcagccag atattgactt attaccattt
     2761 ggagatcaaa ctgaaattgg agagaggggc atcaacctga gtgggggaca gaggcagaga
     2821 atctgtgtgg cacgagcgct gtatcaaaac accaacattg tctttttgga tgatccattc
     2881 tcagccctgg acattcactt gagtgatcat ttaatgcagg aggggatttt gaaattcctg
     2941 caagatgaca aaaggacact cgttcttgtg actcacaaat tacagtatct gacgcatgct
     3001 gactggatca tagccatgaa agatggaagt gtcctaagag aaggaacttt gaaggacatt
     3061 caaaccaaag atgttgagct ttatgaacac tggaaaacac ttatgaatcg gcaagatcaa
     3121 gaattagaaa aggatatgga agctgaccaa actactttag agaggaaaac tctccgacgg
     3181 gccatgtatt caagagaagc caaagcccag atggaggacg aagacgaaga ggaagaagag
     3241 gaggaagatg aggatgataa catgtccact gtaatgaggc tcaggactaa aatgccatgg
     3301 aaaacctgct ggcgctacct gacatctgga ggattcttcc tgctcatcct gatgattttc
     3361 tctaagcttt tgaagcattc ggtcattgta gctatagact attggctggc cacatggaca
     3421 tcggagtaca gtataaacaa tactggaaaa gctgatcaga cctactatgt ggctggcttt
     3481 agcatactct gtggagcagg cattttcctt tgccttgtta catccctcac tgtagaatgg
     3541 atgggtctca cagctgccaa aaatcttcac cacaaccttc tcaataagat aatccttgga
     3601 ccaataaggt tttttgatac cacacccctg ggactgattc tcaatcgctt ttcagctgat
     3661 actaatatca ttgatcagca catccctcca accttggaat ctctaactcg ctcaacactg
     3721 ctctgcctgt ctgccattgg gatgatttct tatgctactc ctgtgttcct ggttgctctc
     3781 ctgccccttg gtgttgcctt ttattttatc cagaaatact ttcgggttgc ctctaaggac
     3841 ctccaggaac ttgacgatag tacccagctc cctctgctct gtcacttctc agaaacagca
     3901 gaaggactca ccaccattcg ggcctttagg catgaaacca gatttaaaca acgtatgctg
     3961 gaactgacgg atacaaacaa cattgcctac ttatttctct cagctgccaa cagatggctg
     4021 gaggtcagga cggattatct gggagcttgc attgtcctca ctgcatctat agcatccatt
     4081 agtgggtctt cgaattctgg attggtaggc ttgggtcttc tgtatgcact tacgataacc
     4141 aattatttga attgggttgt gaggaacttg gctgacctgg aggtccagat gggtgcagtg
     4201 aagaaggtga acagtttcct gactatggag tcagagaact atgaaggcac aatggatcct
     4261 tctcaagttc cagaacattg gccacaagaa ggggagatca agatacatga tctgtgtgtc
     4321 agatatgaaa ataatctgaa acctgttctt aagcacgtca aggcttacat caaacctgga
     4381 caaaaggtgg gcatatgtgg tcgcactggc agtgggaaat catcgttatc tctggctttc
     4441 ttcagaatgg ttgatatatt tgatggaaaa attgtcattg atgggataga catttccaaa
     4501 ttaccactgc acacactacg ttctagactt tcaatcattc tgcaggatcc aatactattc
     4561 agtggttcca ttagatttaa tttagatcca gagtgcaaat gcacagatga cagactctgg
     4621 gaagccttag aaattgctca gctgaagaat atggtcaaat ctctacctgg aggtctagat
     4681 gcggttgtca ctgaaggtgg ggagaatttt agcgttggac agagacagct attttgcctt
     4741 gccagggcct ttgtccgcaa aagcagcatt cttattatgg atgaggcaac agcttccatt
     4801 gacatggcca cagagaatat tttgcaaaaa gtagtaatga cagcctttgc agaccggacc
     4861 gtggtgacaa tagctcatcg agtacacact attctgacgg cagacctggt tattgtgatg
     4921 aagcgaggaa atattttaga atatgacact ccagaaagcc tcttggctca ggaaaatgga
     4981 gtatttgctt cttttgttcg cgcagacatg tgaaggagtg tcttaaaaca atacatgtat
     5041 ttaaaataat gcagtcataa cctaattaat ggatcatcag cttgacctct gtaaagtggc
     5101 atcttaaaac ttttacagat ttttgcacaa aaagtggaca tttatttttc ctgtttttaa
     5161 aacagttttt caagatatta ttgcttaata tgttcattat tgatgttttt atagtgatcc
     5221 agccactcta gctttaagaa tagctaggtt gtattaaaac atgtaagcta ctttaagtga
     5281 taaatcaata gctaaagttt tctcctaact ttaagtctgg taaatatctc tcaggaatgg
     5341 aagtgtcaat gagtagtcag tattactata ctcttgtttg taagaatata ttttccttat
     5401 tgttggagtc tccagaggtt attggaagga ataaaaaagg acatgttttc taacgaaact
     5461 ctcaaattca ttatctactt catgagaatt tatttgtatt cattaacatt agtaatacct
     5521 atttttctta agagccttct tttttccctt ttgtttcttg tcaccaagaa gttatcacac
     5581 ttatacttat tctataaggc caaagcctta tctagaaaga tcatgaagag tttggatatt
     5641 tttaaaacta tactcgaatt ttcatttttt cctgtcctgt aaagagagaa ttcttaagat
     5701 atcaggcata atgtatgtac cttttccaga ctgaagttca aagaatgatt aggttcaatc
     5761 tttcagtggc aggccagcta aactttctat agtttaatga ttagctgtgg ttatgatctt
     5821 gagccctaac atgccacatt actcaagatt tacacaggca agtgacttca tttggcactg
     5881 ctaataatgg caggtgaaca ttcctctcag gtgtgagata tttcctatga aacagtccac
     5941 tggcaggctg gagatatctt acactttgaa gttatatata tataaaacaa gtgtctattt
     6001 ctagctgaaa gagtttacct cttctagaca tttatgccca atggtgtcac acatgatcat
     6061 ttgctcctgc tctaatttta atgtgcatac aaaaaatata ccaaaaatgt tattgtaatt
     6121 ttaatgagca tgtaaagagt gtatcaaaaa ttgatgggat attagtgtta ggttttgctt
     6181 aaatgaaaat taataaattc aaaaatacta aaagtcacct tctttgtcac gttagcaacc
     6241 agtttctact attctgtgcc catccagtgt caatatcatt gatttctttt tcatatgaag
     6301 atccacatgc attgtaagat tgtgaagata tataaaattg atgaattaag agaaattatg
     6361 ccactttcct atagtttctg tgttctgttt attgtatggt gtattcactc ttggaaaata
     6421 tgtaatagtt gtgtatccag aatatgtgtc ttcacctgaa tatttaatct ttatatttac
     6481 aaaaaaataa gttccacaag atggacaaaa atggtccatg atatttagct taaattgtaa
     6541 ccttttgttg tgtatataaa acttccttgg aagaaaataa ccttattgtt ttcttaaagc
     6601 taaacagaat aggattggta ctcattaatt atgtcttgca aatattttct aaaaattcaa
     6661 ccactctcct aaaatggtgt ttaaatattt tgtagggcag aatatgcata ttgcctgtta
     6721 gcaagtaata attcattctc atagagaaat gggtttttac ccatggaagt aatctttttt
     6781 ttttcttttt tttttttaat ttttttttta atatacttta agttttaggg tacatgtgca
     6841 cattgtgcag gttagttaca tatatataca tgtgccatgc tggtgcgctg cacccactaa
     6901 ctcgtcatct agcattaggt atatctccca atgctatccc tccccactcc ccccacccca
     6961 ccacagtccc cagagtgtga tattcccctt cctgtgtcca tgtgatctca ttgttcaatt
     7021 cccacctatg agtgagaata tgcggtgttt ggttttttgt tcttgcgata gtttactgag
     7081 aatgatggtt tccagtttca tccatgtccc tacaaaggac atgaactcat cattttgtat
     7141 ggctgcatag tattccatgg tgtatatgtg ccacattttc ttaatccagt ctatcattgt
     7201 tggacatttg ggttggttcc aagtctttgc tattgtgaat aatgccgcaa taaacatacg
     7261 tgtgcatgtg tctttatagc agcatgattt atagtcattt gggtatatac ccagtaatgg
     7321 gatggctggg tcaaatggta tttctagttc tagatccctg aggaatcgcc acactgactt
     7381 ccacaatggt taaactaaag agcttctgca cagcaaaaga aactaccatc agagtgaaca
     7441 ggcaacctac aacatgggag aaaattttcg caacctactc atctgacaaa gggctaatat
     7501 ccagaatcta caatgaactc aaacaaattt acaagaaaaa aacaaacaac cccatcaaaa
     7561 agtgggtgaa ggacatgaac agacacttct caaaagaaga catttatgca gccaaaaaac
     7621 acatgaaaaa atgctcatca tcactggcca tcagagaaat gcaaatcaaa accactatga
     7681 gatatcatct cacaccagtt agaatggcaa tcattaaaaa gtcaggaaac aacaggtgct
     7741 ggagaggatg tggagaaata ggaacacttt tacactgttg gtgggactgt aaactagttc
     7801 aaccattgtg gaagtaatct tttaatgata ttttcttatt atattgctac aggcaacatc
     7861 agtagaagcc tatctagtta cactaaacta aagatcacat tcaccattct tacagccaag
     7921 tggttgctgg tcccctcata acttttagtt acttaagttg agtaaatgtc taattgttta
     7981 caaactaaat tgactaaata aggtcaagga taatgttttt cattgattaa ccatttatta
     8041 tacatataat ctattgaata taaaaagatt caccttgaaa taatatttga ctatataggt
     8101 acatatctgt aaaagatgac caccttaact aaatcagatt ttacatttat aaattttata
     8161 tttaattagt caaatcaatt tttaaatcat aactccttaa tctcataaga agcaataaat
     8221 gcataaagca ttatattaaa ataccagggt tacttatgtc attgacaggg aaaagacttt
     8281 gtataaacaa ttatgcacca tctatttttg tactcttact catgatccat ttaaaagtct
     8341 actcagctct agattagata ccagaagacc ctctaagtaa ctgataactg agattcggga
     8401 ccatgtcttg tttgccattg tgcccttgga atttaataca agtgcctaac ccacagtagg
     8461 gttgattgat tcactgatta gttttactgc catgtgccta taactatagg ttattctctt
     8521 tccttttcta tgttgtgttt attctattta ttctctacaa tatataaaat gtatttgttc
     8581 tctacaataa atgtgtttat tctctacaat atataaaaac atattggact actattacat
     8641 aattaaatta ctcttttatt tgcttaaa
//
