LOCUS       NM_000352               4921 bp    mRNA    linear   PRI 03-DEC-2024
DEFINITION  Homo sapiens ATP binding cassette subfamily C member 8 (ABCC8),
            transcript variant 2, mRNA.
ACCESSION   NM_000352
VERSION     NM_000352.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Patton,B.L., Zhu,P., ElSheikh,A., Driggers,C.M. and Shyng,S.L.
  TITLE     Dynamic duo: Kir6 and SUR in KATP channel structure and function
  JOURNAL   Channels (Austin) 18 (1), 2327708 (2024)
   PUBMED   38489043
  REMARK    GeneRIF: Dynamic duo: Kir6 and SUR in KATP channel structure and
            function.
            Review article
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Butnariu,L.I., Bizim,D.A., Paduraru,G., Paduraru,L., Moisa,S.M.,
            Popa,S., Gimiga,N., Ghiga,G., Badescu,M.C., Lupu,A., Vasiliu,I. and
            Trandafir,L.M.
  TITLE     Congenital Hyperinsulinism Caused by Mutations in ABCC8 Gene
            Associated with Early-Onset Neonatal Hypoglycemia: Genetic
            Heterogeneity Correlated with Phenotypic Variability
  JOURNAL   Int J Mol Sci 25 (10), 5533 (2024)
   PUBMED   38791571
  REMARK    GeneRIF: Congenital Hyperinsulinism Caused by Mutations in ABCC8
            Gene Associated with Early-Onset Neonatal Hypoglycemia: Genetic
            Heterogeneity Correlated with Phenotypic Variability.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4921)
  AUTHORS   Alp,E., Doguizi,S., Mutlu Icduygu,F., Akgun,E., Sekeroglu,M.A. and
            Ozer,M.A.
  TITLE     An analysis of the relationship between ABCC8 and KCNJ11 gene
            polymorphisms and diabetic retinopathy in Turkish population
  JOURNAL   Ophthalmic Genet 45 (2), 126-132 (2024)
   PUBMED   38411150
  REMARK    GeneRIF: An analysis of the relationship between ABCC8 and KCNJ11
            gene polymorphisms and diabetic retinopathy in Turkish population.
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   Bryan,J., Munoz,A., Zhang,X., Dufer,M., Drews,G., Krippeit-Drews,P.
            and Aguilar-Bryan,L.
  TITLE     ABCC8 and ABCC9: ABC transporters that regulate K+ channels
  JOURNAL   Pflugers Arch 453 (5), 703-718 (2007)
   PUBMED   16897043
  REMARK    Review article
REFERENCE   5  (bases 1 to 4921)
  AUTHORS   Inagaki,N., Gonoi,T., Clement,J.P. 4th, Namba,N., Inazawa,J.,
            Gonzalez,G., Aguilar-Bryan,L., Seino,S. and Bryan,J.
  TITLE     Reconstitution of IKATP: an inward rectifier subunit plus the
            sulfonylurea receptor
  JOURNAL   Science 270 (5239), 1166-1170 (1995)
   PUBMED   7502040
REFERENCE   6  (bases 1 to 4921)
  AUTHORS   Thomas,P.M., Cote,G.J., Wohllk,N., Haddad,B., Mathew,P.M., Rabl,W.,
            Aguilar-Bryan,L., Gagel,R.F. and Bryan,J.
  TITLE     Mutations in the sulfonylurea receptor gene in familial persistent
            hyperinsulinemic hypoglycemia of infancy
  JOURNAL   Science 268 (5209), 426-429 (1995)
   PUBMED   7716548
REFERENCE   7  (bases 1 to 4921)
  AUTHORS   Aguilar-Bryan,L., Nichols,C.G., Wechsler,S.W., Clement,J.P. 4th,
            Boyd,A.E. 3rd, Gonzalez,G., Herrera-Sosa,H., Nguy,K., Bryan,J. and
            Nelson,D.A.
  TITLE     Cloning of the beta cell high-affinity sulfonylurea receptor: a
            regulator of insulin secretion
  JOURNAL   Science 268 (5209), 423-426 (1995)
   PUBMED   7716547
REFERENCE   8  (bases 1 to 4921)
  AUTHORS   Naylor,R., Knight Johnson,A. and del Gaudio,D.
  TITLE     Maturity-Onset Diabetes of the Young Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29792621
REFERENCE   9  (bases 1 to 4921)
  AUTHORS   De Leon,D.D. and Pinney,S.E.
  TITLE     Permanent Neonatal Diabetes Mellitus
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301620
REFERENCE   10 (bases 1 to 4921)
  AUTHORS   Gillis,D.
  TITLE     Nonsyndromic Genetic Hyperinsulinism Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301549
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124798.5, AF087138.1,
            R84665.1, BM352492.1 and BM313666.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 8, 2019 this sequence version replaced NM_000352.5.
            
            Summary: The protein encoded by this gene is a member of the
            superfamily of ATP-binding cassette (ABC) transporters. ABC
            proteins transport various molecules across extra- and
            intra-cellular membranes. ABC genes are divided into seven distinct
            subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This
            protein is a member of the MRP subfamily which is involved in
            multi-drug resistance. This protein functions as a modulator of
            ATP-sensitive potassium channels and insulin release. Mutations in
            the ABCC8 gene and deficiencies in the encoded protein have been
            observed in patients with hyperinsulinemic hypoglycemia of infancy,
            an autosomal recessive disorder of unregulated and high insulin
            secretion. Mutations have also been associated with
            non-insulin-dependent diabetes mellitus type II, an autosomal
            dominant disease of defective insulin secretion. Alternatively
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Jul 2020].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 3. The
            encoded isoform (2) is shorter than isoform 3. Isoforms 2 and 4 are
            the same length but differ in sequence.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L78207.1, AF087138.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145313 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000389817.8/ ENSP00000374467.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC124798.5         126650-126702       c
            54-1754             AF087138.1         20-1720
            1755-1755           R84665.1           78-78
            1756-4173           AF087138.1         1722-4139
            4174-4174           BM352492.1         34-34               c
            4175-4860           AF087138.1         4141-4826
            4861-4921           BM313666.1         15-75               c
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.1"
     gene            1..4921
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /note="ATP binding cassette subfamily C member 8"
                     /db_xref="GeneID:6833"
                     /db_xref="HGNC:HGNC:59"
                     /db_xref="MIM:600509"
     exon            1..217
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            218..359
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            360..481
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            482..648
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..891
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1080
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1245
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1401
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1536
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1699
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1740
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1886
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1992
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2109
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2185
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2291
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2324
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2360
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2459
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2544
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2625
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2763
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2764..2889
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..2989
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            2990..3231
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3232..3398
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3399..3468
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3626
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3627..3719
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3720..3822
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3823..3936
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            3937..4057
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4058..4188
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4267
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4268..4376
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4377..4480
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4481..4614
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4615..4677
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     exon            4678..4921
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4897..4902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /note="hexamer: AATAAA"
     polyA_site      4921
                     /gene="ABCC8"
                     /gene_synonym="ABC36; HHF1; HI; HRINS; MRP8; PHHI; PNDM3;
                     SUR; SUR1; SUR1delta2; TNDM2"
                     /note="major polyA site"
ORIGIN      
        1 agaggcgcgg caggcgcgcg gagccagcgg agccagctga gcccgagccc agcccgcgcc
       61 cgcgccgcca tgcccctggc cttctgcggc agcgagaacc actcggccgc ctaccgggtg
      121 gaccaggggg tcctcaacaa cggctgcttt gtggacgcgc tcaacgtggt gccgcacgtc
      181 ttcctactct tcatcacctt ccccatcctc ttcattggat ggggaagtca gagctccaag
      241 gtgcacatcc accacagcac atggcttcat ttccctgggc acaacctgcg gtggatcctg
      301 accttcatgc tgctcttcgt cctggtgtgt gagattgcag agggcatcct gtctgatggg
      361 gtgaccgaat cccaccatct gcacctgtac atgccagccg ggatggcgtt catggctgct
      421 gtcacctccg tggtctacta tcacaacatc gagacttcca acttccccaa gctgctaatt
      481 gccctgctgg tgtattggac cctggccttc atcaccaaga ccatcaagtt tgtcaagttc
      541 ttggaccacg ccatcggctt ctcgcagcta cgcttctgcc tcacagggct gctggtgatc
      601 ctctatggga tgctgctcct cgtggaggtc aatgtcatca gggtgaggag atacatcttc
      661 ttcaagacac cgagggaggt gaagcctccc gaggacctgc aagacctggg ggtacgcttc
      721 ctgcagccct tcgtgaatct gctgtccaaa ggcacctact ggtggatgaa cgccttcatc
      781 aagactgccc acaagaagcc catcgacttg cgagccatcg ggaagctgcc catcgccatg
      841 agggccctca ccaactacca acggctctgc gaggcctttg acgcccaggt gcggaaggac
      901 attcagggca ctcaaggtgc ccgggccatc tggcaggcac tcagccatgc cttcgggagg
      961 cgcctggtcc tcagcagcac tttccgcatc ttggccgacc tgctgggctt cgccgggcca
     1021 ctgtgcatct ttgggatcgt ggaccacctt gggaaggaga acgacgtctt ccagcccaag
     1081 acacaatttc tcggggttta ctttgtctca tcccaagagt tccttgccaa tgcctacgtc
     1141 ttagctgtgc ttctgttcct tgccctccta ctgcaaagga catttctgca agcatcctac
     1201 tatgtggcca ttgaaactgg aattaacttg agaggagcaa tacagaccaa gatttacaat
     1261 aaaattatgc acctgtccac ctccaacctg tccatgggag aaatgactgc tggacagatc
     1321 tgtaatctgg ttgccatcga caccaatcag ctcatgtggt ttttcttctt gtgcccaaac
     1381 ctctgggcta tgccagtaca gatcattgtg ggtgtgattc tcctctacta catactcgga
     1441 gtcagtgcct taattggagc agctgtcatc attctactgg ctcctgtcca gtacttcgtg
     1501 gccaccaagc tgtctcaggc ccagcggagc acactggagt attccaatga gcggctgaag
     1561 cagaccaacg agatgctccg cggcatcaag ctgctgaagc tgtacgcctg ggagaacatc
     1621 ttccgcacgc gggtggagac gacccgcagg aaggagatga ccagcctcag ggcctttgcc
     1681 atctatacct ccatctccat tttcatgaac acggccatcc ccattgcagc tgtcctcata
     1741 actttcgtgg gccacgtcag cttcttcaaa gaggccgact tctcgccctc cgtggccttt
     1801 gcctccctct ccctcttcca tatcttggtc acaccgctgt tcctgctgtc cagtgtggtc
     1861 cgatctaccg tcaaagctct agtgagcgtg caaaagctaa gcgagttcct gtccagtgca
     1921 gagatccgtg aggagcagtg tgccccccat gagcccacac ctcagggccc agccagcaag
     1981 taccaggcgg tgcccctcag ggttgtgaac cgcaagcgtc cagcccggga ggattgtcgg
     2041 ggcctcaccg gcccactgca gagcctggtc cccagtgcag atggcgatgc tgacaactgc
     2101 tgtgtccaga tcatgggagg ctacttcacg tggaccccag atggaatccc cacactgtcc
     2161 aacatcacca ttcgtatccc ccgaggccag ctgactatga tcgtggggca ggtgggctgc
     2221 ggcaagtcct cgctccttct agccgcactg ggggagatgc agaaggtctc aggggctgtc
     2281 ttctggagca gccttcctga cagcgagata ggagaggacc ccagcccaga gcgggagaca
     2341 gcgaccgact tggatatcag gaagagaggc cccgtggcct atgcttcgca gaaaccatgg
     2401 ctgctaaatg ccactgtgga ggagaacatc atctttgaga gtcccttcaa caaacaacgg
     2461 tacaagatgg tcattgaagc ctgctctctg cagccagaca tcgacatcct gccccatgga
     2521 gaccagaccc agattgggga acggggcatc aacctgtctg gtggtcaacg ccagcgaatc
     2581 agtgtggccc gagccctcta ccagcacgcc aacgttgtct tcttggatga ccccttctca
     2641 gctctggata tccatctgag tgaccactta atgcaggccg gcatccttga gctgctccgg
     2701 gacgacaaga ggacagtggt cttagtgacc cacaagctac agtacctgcc ccatgcagac
     2761 tggatcattg ccatgaagga tggcaccatc cagagggagg gtaccctcaa ggacttccag
     2821 aggtctgaat gccagctctt tgagcactgg aagaccctca tgaaccgaca ggaccaagag
     2881 ctggagaagg agactgtcac agagagaaaa gccacagagc caccccaggg cctatctcgt
     2941 gccatgtcct cgagggatgg ccttctgcag gatgaggaag aggaggaaga ggaggcagct
     3001 gagagcgagg aggatgacaa cctgtcgtcc atgctgcacc agcgtgctga gatcccatgg
     3061 cgagcctgcg ccaagtacct gtcctccgcc ggcatcctgc tcctgtcgtt gctggtcttc
     3121 tcacagctgc tcaagcacat ggtcctggtg gccatcgact actggctggc caagtggacc
     3181 gacagcgccc tgaccctgac ccctgcagcc aggaactgct ccctcagcca ggagtgcacc
     3241 ctcgaccaga ctgtctatgc catggtgttc acggtgctct gcagcctggg cattgtgctg
     3301 tgcctcgtca cgtctgtcac tgtggagtgg acagggctga aggtggccaa gagactgcac
     3361 cgcagcctgc taaaccggat catcctagcc cccatgaggt tttttgagac cacgcccctt
     3421 gggagcatcc tgaacagatt ttcatctgac tgtaacacca tcgaccagca catcccatcc
     3481 acgctggagt gcctgagccg ctccaccctg ctctgtgtct cagccctggc cgtcatctcc
     3541 tatgtcacac ctgtgttcct cgtggccctc ttgcccctgg ccatcgtgtg ctacttcatc
     3601 cagaagtact tccgggtggc gtccagggac ctgcagcagc tggatgacac cacccagctt
     3661 ccacttctct cacactttgc cgaaaccgta gaaggactca ccaccatccg ggccttcagg
     3721 tatgaggccc ggttccagca gaagcttctc gaatacacag actccaacaa cattgcttcc
     3781 ctcttcctca cagctgccaa cagatggctg gaagtccgaa tggagtacat cggtgcatgt
     3841 gtggtgctca tcgcagcggt gacctccatc tccaactccc tgcacaggga gctctctgct
     3901 ggcctggtgg gcctgggcct tacctacgcc ctaatggtct ccaactacct caactggatg
     3961 gtgaggaacc tggcagacat ggagctccag ctgggggctg tgaagcgcat ccatgggctc
     4021 ctgaaaaccg aggcagagag ctacgagggg ctcctggcac catcgctgat cccaaagaac
     4081 tggccagacc aagggaagat ccagatccag aacctgagcg tgcgctacga cagctccctg
     4141 aagccggtgc tgaagcacgt caatgccctc atcgcccctg gacagaagat cgggatctgc
     4201 ggccgcaccg gcagtgggaa gtcctccttc tctcttgcct tcttccgcat ggtggacacg
     4261 ttcgaagggc acatcatcat tgatggcatt gacatcgcca aactgccgct gcacaccctg
     4321 cgctcacgcc tctccatcat cctgcaggac cccgtcctct tcagcggcac catccgattt
     4381 aacctggacc ctgagaggaa gtgctcagat agcacactgt gggaggccct ggaaatcgcc
     4441 cagctgaagc tggtggtgaa ggcactgcca ggaggcctcg atgccatcat cacagaaggc
     4501 ggggagaatt tcagccaggg acagaggcag ctgttctgcc tggcccgggc cttcgtgagg
     4561 aagaccagca tcttcatcat ggacgaggcc acggcttcca ttgacatggc cacggaaaac
     4621 atcctccaaa aggtggtgat gacagccttc gcagaccgca ctgtggtcac catcgcgcat
     4681 cgagtgcaca ccatcctgag tgcagacctg gtgatcgtcc tgaagcgggg tgccatcctt
     4741 gagttcgata agccagagaa gctgctcagc cggaaggaca gcgtcttcgc ctccttcgtc
     4801 cgtgcagaca agtgacctgc cagagcccaa gtgccatccc acattcggac cctgcccata
     4861 cccctgcctg ggttttctaa ctgtaaatca cttgtaaata aatagatttg attatttcct
     4921 a
//
