LOCUS       NM_000392               5806 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ATP binding cassette subfamily C member 2 (ABCC2),
            mRNA.
ACCESSION   NM_000392
VERSION     NM_000392.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Nakayama,H., Ishida,H., Iidaka,M., Kato,S., Nakatani,K.,
            Nakayama,A., Noguchi,T., Nishihara,S., Oikawa,S., Usami,T.,
            Mitsui,Y., Ishii,Y.U., Toshima,H., Kobayashi,K., Murase,R.,
            Matsumoto,N., Suzuki,K., Shimada,K., Yoshida,H. and Fujita,K.I.
  TITLE     Association Between ABCC2 -24C>T and Nab-Paclitaxel-induced
            Peripheral Neuropathy in Japanese Patients With Pancreatic Cancer
  JOURNAL   Anticancer Res 44 (11), 5023-5033 (2024)
   PUBMED   39477313
  REMARK    GeneRIF: Association Between ABCC2 -24C>T and
            Nab-Paclitaxel-induced Peripheral Neuropathy in Japanese Patients
            With Pancreatic Cancer.
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Kolz,C., Gaugaz,F.Z., Handin,N., Schaeffeler,E., Tremmel,R.,
            Winter,S., Klein,K., Zanger,U.M., Artursson,P., Schwab,M. and
            Nies,A.T.
  TITLE     In silico and biological analyses of missense variants of the human
            biliary efflux transporter ABCC2: effects of novel rare missense
            variants
  JOURNAL   Br J Pharmacol 181 (22), 4593-4609 (2024)
   PUBMED   39096023
  REMARK    GeneRIF: In silico and biological analyses of missense variants of
            the human biliary efflux transporter ABCC2: effects of novel rare
            missense variants.
REFERENCE   3  (bases 1 to 5806)
  AUTHORS   Wang,Y., Gan,X., Cheng,X., Jia,Y., Wang,G., Tang,X., Du,H., Li,X.,
            Liu,X., Xing,X., Ji,J. and Li,Z.
  TITLE     ABCC2 induces metabolic vulnerability and cellular ferroptosis via
            enhanced glutathione efflux in gastric cancer
  JOURNAL   Clin Transl Med 14 (8), e1754 (2024)
   PUBMED   39095325
  REMARK    GeneRIF: ABCC2 induces metabolic vulnerability and cellular
            ferroptosis via enhanced glutathione efflux in gastric cancer.
REFERENCE   4  (bases 1 to 5806)
  AUTHORS   Mao,Y.X., Chen,Z.P., Wang,L., Wang,J., Zhou,C.Z., Hou,W.T. and
            Chen,Y.
  TITLE     Transport mechanism of human bilirubin transporter ABCC2 tuned by
            the inter-module regulatory domain
  JOURNAL   Nat Commun 15 (1), 1061 (2024)
   PUBMED   38316776
  REMARK    GeneRIF: Transport mechanism of human bilirubin transporter ABCC2
            tuned by the inter-module regulatory domain.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5806)
  AUTHORS   Zhang,Y., Zhao,T., Li,W. and Vore,M.
  TITLE     The 5'-untranslated region of multidrug resistance associated
            protein 2 (MRP2; ABCC2) regulates downstream open reading frame
            expression through translational regulation
  JOURNAL   Mol Pharmacol 77 (2), 237-246 (2010)
   PUBMED   19890061
  REMARK    GeneRIF: The 5'-untranslated region of MRP2 markedly influences
            MRP2 mRNA translation regulation.
REFERENCE   6  (bases 1 to 5806)
  AUTHORS   Tanaka,T., Uchiumi,T., Hinoshita,E., Inokuchi,A., Toh,S., Wada,M.,
            Takano,H., Kohno,K. and Kuwano,M.
  TITLE     The human multidrug resistance protein 2 gene: functional
            characterization of the 5'-flanking region and expression in
            hepatic cells
  JOURNAL   Hepatology 30 (6), 1507-1512 (1999)
   PUBMED   10573531
REFERENCE   7  (bases 1 to 5806)
  AUTHORS   Allikmets,R., Gerrard,B., Hutchinson,A. and Dean,M.
  TITLE     Characterization of the human ABC superfamily: isolation and
            mapping of 21 new genes using the expressed sequence tags database
  JOURNAL   Hum Mol Genet 5 (10), 1649-1655 (1996)
   PUBMED   8894702
REFERENCE   8  (bases 1 to 5806)
  AUTHORS   Taniguchi,K., Wada,M., Kohno,K., Nakamura,T., Kawabe,T.,
            Kawakami,M., Kagotani,K., Okumura,K., Akiyama,S. and Kuwano,M.
  TITLE     A human canalicular multispecific organic anion transporter (cMOAT)
            gene is overexpressed in cisplatin-resistant human cancer cell
            lines with decreased drug accumulation
  JOURNAL   Cancer Res 56 (18), 4124-4129 (1996)
   PUBMED   8797578
REFERENCE   9  (bases 1 to 5806)
  AUTHORS   Buchler,M., Konig,J., Brom,M., Kartenbeck,J., Spring,H., Horie,T.
            and Keppler,D.
  TITLE     cDNA cloning of the hepatocyte canalicular isoform of the multidrug
            resistance protein, cMrp, reveals a novel conjugate export pump
            deficient in hyperbilirubinemic mutant rats
  JOURNAL   J Biol Chem 271 (25), 15091-15098 (1996)
   PUBMED   8662992
REFERENCE   10 (bases 1 to 5806)
  AUTHORS   Mayer,R., Kartenbeck,J., Buchler,M., Jedlitschky,G., Leier,I. and
            Keppler,D.
  TITLE     Expression of the MRP gene-encoded conjugate export pump in liver
            and its selective absence from the canalicular membrane in
            transport-deficient mutant hepatocytes
  JOURNAL   J Cell Biol 131 (1), 137-150 (1995)
   PUBMED   7559771
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133353.6, U49248.1, U63970.1
            and AL392107.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 10, 2018 this sequence version replaced NM_000392.4.
            
            Summary: The protein encoded by this gene is a member of the
            superfamily of ATP-binding cassette (ABC) transporters. ABC
            proteins transport various molecules across extra- and
            intra-cellular membranes. ABC genes are divided into seven distinct
            subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This
            protein is a member of the MRP subfamily which is involved in
            multi-drug resistance. This protein is expressed in the canalicular
            (apical) part of the hepatocyte and functions in biliary transport.
            Substrates include anticancer drugs such as vinblastine; therefore,
            this protein appears to contribute to drug resistance in mammalian
            cells. Several different mutations in this gene have been observed
            in patients with Dubin-Johnson syndrome (DJS), an autosomal
            recessive disorder characterized by conjugated hyperbilirubinemia.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853560.4000.1, U49248.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2144835
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000647814.1/ ENSP00000497274.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            regulatory uORF        :: PMID: 19890061
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-106               AL133353.6         131202-131307
            107-320             U49248.1           3-216
            321-321             AL133353.6         133252-133252
            322-3767            U49248.1           218-3663
            3768-5028           U63970.1           3600-4860
            5029-5806           AL392107.17        43183-43960
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q24.2"
     gene            1..5806
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /note="ATP binding cassette subfamily C member 2"
                     /db_xref="GeneID:1244"
                     /db_xref="HGNC:HGNC:53"
                     /db_xref="MIM:601107"
     exon            1..238
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      101..169
                     /regulatory_class="uORF"
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19890061]"
                     /note="this uORF is predicted to encode a 22 aa peptide
                     (PMID:19890061)"
     CDS             206..4843
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /EC_number="7.6.2.2"
                     /EC_number="7.6.2.3"
                     /note="canalicular multispecific organic anion transporter
                     1; canalicular multidrug resistance protein; multidrug
                     resistance-associated protein 2; ATP-binding cassette,
                     sub-family C (CFTR/MRP), member 2"
                     /codon_start=1
                     /product="ATP-binding cassette sub-family C member 2"
                     /protein_id="NP_000383.2"
                     /db_xref="CCDS:CCDS7484.1"
                     /db_xref="GeneID:1244"
                     /db_xref="HGNC:HGNC:53"
                     /db_xref="MIM:601107"
                     /translation="MLEKFCNSTFWNSSFLDSPEADLPLCFEQTVLVWIPLGYLWLLA
                     PWQLLHVYKSRTKRSSTTKLYLAKQVFVGFLLILAAIELALVLTEDSGQATVPAVRYT
                     NPSLYLGTWLLVLLIQYSRQWCVQKNSWFLSLFWILSILCGTFQFQTLIRTLLQGDNS
                     NLAYSCLFFISYGFQILILIFSAFSENNESSNNPSSIASFLSSITYSWYDSIILKGYK
                     RPLTLEDVWEVDEEMKTKTLVSKFETHMKRELQKARRALQRRQEKSSQQNSGARLPGL
                     NKNQSQSQDALVLEDVEKKKKKSGTKKDVPKSWLMKALFKTFYMVLLKSFLLKLVNDI
                     FTFVSPQLLKLLISFASDRDTYLWIGYLCAILLFTAALIQSFCLQCYFQLCFKLGVKV
                     RTAIMASVYKKALTLSNLARKEYTVGETVNLMSVDAQKLMDVTNFMHMLWSSVLQIVL
                     SIFFLWRELGPSVLAGVGVMVLVIPINAILSTKSKTIQVKNMKNKDKRLKIMNEILSG
                     IKILKYFAWEPSFRDQVQNLRKKELKNLLAFSQLQCVVIFVFQLTPVLVSVVTFSVYV
                     LVDSNNILDAQKAFTSITLFNILRFPLSMLPMMISSMLQASVSTERLEKYLGGDDLDT
                     SAIRHDCNFDKAMQFSEASFTWEHDSEATVRDVNLDIMAGQLVAVIGPVGSGKSSLIS
                     AMLGEMENVHGHITIKGTTAYVPQQSWIQNGTIKDNILFGTEFNEKRYQQVLEACALL
                     PDLEMLPGGDLAEIGEKGINLSGGQKQRISLARATYQNLDIYLLDDPLSAVDAHVGKH
                     IFNKVLGPNGLLKGKTRLLVTHSMHFLPQVDEIVVLGNGTIVEKGSYSALLAKKGEFA
                     KNLKTFLRHTGPEEEATVHDGSEEEDDDYGLISSVEEIPEDAASITMRRENSFRRTLS
                     RSSRSNGRHLKSLRNSLKTRNVNSLKEDEELVKGQKLIKKEFIETGKVKFSIYLEYLQ
                     AIGLFSIFFIILAFVMNSVAFIGSNLWLSAWTSDSKIFNSTDYPASQRDMRVGVYGAL
                     GLAQGIFVFIAHFWSAFGFVHASNILHKQLLNNILRAPMRFFDTTPTGRIVNRFAGDI
                     STVDDTLPQSLRSWITCFLGIISTLVMICMATPVFTIIVIPLGIIYVSVQMFYVSTSR
                     QLRRLDSVTRSPIYSHFSETVSGLPVIRAFEHQQRFLKHNEVRIDTNQKCVFSWITSN
                     RWLAIRLELVGNLTVFFSALMMVIYRDTLSGDTVGFVLSNALNITQTLNWLVRMTSEI
                     ETNIVAVERITEYTKVENEAPWVTDKRPPPDWPSKGKIQFNNYQVRYRPELDLVLRGI
                     TCDIGSMEKIGVVGRTGAGKSSLTNCLFRILEAAGGQIIIDGVDIASIGLHDLREKLT
                     IIPQDPILFSGSLRMNLDPFNNYSDEEIWKALELAHLKSFVASLQLGLSHEVTEAGGN
                     LSIGQRQLLCLGRALLRKSKILVLDEATAAVDLETDNLIQTTIQNEFAHCTVITIAHR
                     LHTIMDSDKVMVLDNGKIIECGSPEELLQIPGPFYFMAKEAGIENVNSTKF"
     exon            239..412
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            413..538
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..673
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            674..781
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..837
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1072
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1236
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1414
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1669
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1735
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1873
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1874..2020
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2105
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2172
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2299
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2476
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2644
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2645..2825
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2952
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3088
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3308
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3463
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3619
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3819
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..3946
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3947..4048
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4049..4192
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4193..4351
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4352..4518
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4519..4713
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            4714..5806
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5765..5770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /note="hexamer: ATTATA"
     polyA_site      5788
                     /gene="ABCC2"
                     /gene_synonym="ABC30; CMOAT; cMRP; DJS; MRP2"
                     /note="major polyA site"
ORIGIN      
        1 agtcacatgt ccatccactg tttcaatgta acatgcatct aggcaaggtt aacgattaaa
       61 tggttgggat gaaaggtcat cctttacgga gaacatcaga atggtagata attcctgttc
      121 cactttcttt gatgaaacaa gtaaagaaga aacaacacaa tcatattaat agaagagtct
      181 tcgttccaga cgcagtccag gaatcatgct ggagaagttc tgcaactcta ctttttggaa
      241 ttcctcattc ctggacagtc cggaggcaga cctgccactt tgttttgagc aaactgttct
      301 ggtgtggatt cccttgggct acctatggct cctggccccc tggcagcttc tccacgtgta
      361 taaatccagg accaagagat cctctaccac caaactctat cttgctaagc aggtattcgt
      421 tggttttctt cttattctag cagccataga gctggccctt gtactcacag aagactctgg
      481 acaagccaca gtccctgctg ttcgatatac caatccaagc ctctacctag gcacatggct
      541 cctggttttg ctgatccaat acagcagaca atggtgtgta cagaaaaact cctggttcct
      601 gtccctattc tggattctct cgatactctg tggcactttc caatttcaga ctctgatccg
      661 gacactctta cagggtgaca attctaatct agcctactcc tgcctgttct tcatctccta
      721 cggattccag atcctgatcc tgatcttttc agcattttca gaaaataatg agtcatcaaa
      781 taatccatca tccatagctt cattcctgag tagcattacc tacagctggt atgacagcat
      841 cattctgaaa ggctacaagc gtcctctgac actcgaggat gtctgggaag ttgatgaaga
      901 gatgaaaacc aagacattag tgagcaagtt tgaaacgcac atgaagagag agctgcagaa
      961 agccaggcgg gcactccaga gacggcagga gaagagctcc cagcagaact ctggagccag
     1021 gctgcctggc ttgaacaaga atcagagtca aagccaagat gcccttgtcc tggaagatgt
     1081 tgaaaagaaa aaaaagaagt ctgggaccaa aaaagatgtt ccaaaatcct ggttgatgaa
     1141 ggctctgttc aaaactttct acatggtgct cctgaaatca ttcctactga agctagtgaa
     1201 tgacatcttc acgtttgtga gtcctcagct gctgaaattg ctgatctcct ttgcaagtga
     1261 ccgtgacaca tatttgtgga ttggatatct ctgtgcaatc ctcttattca ctgcggctct
     1321 cattcagtct ttctgccttc agtgttattt ccaactgtgc ttcaagctgg gtgtaaaagt
     1381 acggacagct atcatggctt ctgtatataa gaaggcattg accctatcca acttggccag
     1441 gaaggagtac accgttggag aaacagtgaa cctgatgtct gtggatgccc agaagctcat
     1501 ggatgtgacc aacttcatgc acatgctgtg gtcaagtgtt ctacagattg tcttatctat
     1561 cttcttccta tggagagagt tgggaccctc agtcttagca ggtgttgggg tgatggtgct
     1621 tgtaatccca attaatgcga tactgtccac caagagtaag accattcagg tcaaaaatat
     1681 gaagaataaa gacaaacgtt taaagatcat gaatgagatt cttagtggaa tcaagatcct
     1741 gaaatatttt gcctgggaac cttcattcag agaccaagta caaaacctcc ggaagaaaga
     1801 gctcaagaac ctgctggcct ttagtcaact acagtgtgta gtaatattcg tcttccagtt
     1861 aactccagtc ctggtatctg tggtcacatt ttctgtttat gtcctggtgg atagcaacaa
     1921 tattttggat gcacaaaagg ccttcacctc cattaccctc ttcaatatcc tgcgctttcc
     1981 cctgagcatg cttcccatga tgatctcctc catgctccag gccagtgttt ccacagagcg
     2041 gctagagaag tacttgggag gggatgactt ggacacatct gccattcgac atgactgcaa
     2101 ttttgacaaa gccatgcagt tttctgaggc ctcctttacc tgggaacatg attcggaagc
     2161 cacagtccga gatgtgaacc tggacattat ggcaggccaa cttgtggctg tgataggccc
     2221 tgtcggctct gggaaatcct ccttgatatc agccatgctg ggagaaatgg aaaatgtcca
     2281 cgggcacatc accatcaagg gcaccactgc ctatgtccca cagcagtcct ggattcagaa
     2341 tggcaccata aaggacaaca tcctttttgg aacagagttt aatgaaaaga ggtaccagca
     2401 agtactggag gcctgtgctc tcctcccaga cttggaaatg ctgcctggag gagatttggc
     2461 tgagattgga gagaagggta taaatcttag tgggggtcag aagcagcgga tcagcctggc
     2521 cagagctacc taccaaaatt tagacatcta tcttctagat gaccccctgt ctgcagtgga
     2581 tgctcatgta ggaaaacata tttttaataa ggtcttgggc cccaatggcc tgttgaaagg
     2641 caagactcga ctcttggtta cacatagcat gcactttctt cctcaagtgg atgagattgt
     2701 agttctgggg aatggaacaa ttgtagagaa aggatcctac agtgctctcc tggccaaaaa
     2761 aggagagttt gctaagaatc tgaagacatt tctaagacat acaggccctg aagaggaagc
     2821 cacagtccat gatggcagtg aagaagaaga cgatgactat gggctgatat ccagtgtgga
     2881 agagatcccc gaagatgcag cctccataac catgagaaga gagaacagct ttcgtcgaac
     2941 acttagccgc agttctaggt ccaatggcag gcatctgaag tccctgagaa actccttgaa
     3001 aactcggaat gtgaatagcc tgaaggaaga cgaagaacta gtgaaaggac aaaaactaat
     3061 taagaaggaa ttcatagaaa ctggaaaggt gaagttctcc atctacctgg agtacctaca
     3121 agcaatagga ttgttttcga tattcttcat catccttgcg tttgtgatga attctgtggc
     3181 ttttattgga tccaacctct ggctcagtgc ttggaccagt gactctaaaa tcttcaatag
     3241 caccgactat ccagcatctc agagggacat gagagttgga gtctacggag ctctgggatt
     3301 agcccaaggt atatttgtgt tcatagcaca tttctggagt gcctttggtt tcgtccatgc
     3361 atcaaatatc ttgcacaagc aactgctgaa caatatcctt cgagcaccta tgagattttt
     3421 tgacacaaca cccacaggcc ggattgtgaa caggtttgcc ggcgatattt ccacagtgga
     3481 tgacaccctg cctcagtcct tgcgcagctg gattacatgc ttcctgggga taatcagcac
     3541 ccttgtcatg atctgcatgg ccactcctgt cttcaccatc atcgtcattc ctcttggcat
     3601 tatttatgta tctgttcaga tgttttatgt gtctacctcc cgccagctga ggcgtctgga
     3661 ctctgtcacc aggtccccaa tctactctca cttcagcgag accgtatcag gtttgccagt
     3721 tatccgtgcc tttgagcacc agcagcgatt tctgaaacac aatgaggtga ggattgacac
     3781 caaccagaaa tgtgtctttt cctggatcac ctccaacagg tggcttgcaa ttcgcctgga
     3841 gctggttggg aacctgactg tcttcttttc agccttgatg atggttattt atagagatac
     3901 cctaagtggg gacactgttg gctttgttct gtccaatgca ctcaatatca cacaaaccct
     3961 gaactggctg gtgaggatga catcagaaat agagaccaac attgtggctg ttgagcgaat
     4021 aactgagtac acaaaagtgg aaaatgaggc accctgggtg actgataaga ggcctccgcc
     4081 agattggccc agcaaaggca agatccagtt taacaactac caagtgcggt accgacctga
     4141 gctggatctg gtcctcagag ggatcacttg tgacatcggt agcatggaga agattggtgt
     4201 ggtgggcagg acaggagctg gaaagtcatc cctcacaaac tgcctcttca gaatcttaga
     4261 ggctgccggt ggtcagatta tcattgatgg agtagatatt gcttccattg ggctccacga
     4321 cctccgagag aagctgacca tcatccccca ggaccccatc ctgttctctg gaagcctgag
     4381 gatgaatctc gaccctttca acaactactc agatgaggag atttggaagg ccttggagct
     4441 ggctcacctc aagtcttttg tggccagcct gcaacttggg ttatcccacg aagtgacaga
     4501 ggctggtggc aacctgagca taggccagag gcagctgctg tgcctgggca gggctctgct
     4561 tcggaaatcc aagatcctgg tcctggatga ggccactgct gcggtggatc tagagacaga
     4621 caacctcatt cagacgacca tccaaaacga gttcgcccac tgcacagtga tcaccatcgc
     4681 ccacaggctg cacaccatca tggacagtga caaggtaatg gtcctagaca acgggaagat
     4741 tatagagtgc ggcagccctg aagaactgct acaaatccct ggaccctttt actttatggc
     4801 taaggaagct ggcattgaga atgtgaacag cacaaaattc tagcagaagg ccccatgggt
     4861 tagaaaagga ctataagaat aatttcttat ttaattttat tttttataaa atacagaata
     4921 catacaaaag tgtgtataaa atgtacgttt taaaaaagga taagtgaaca cccatgaacc
     4981 tactacccag gttaagaaaa taaatgtcac caggtacttg agaaacccct cgattgtcta
     5041 cctcgatcgt acttccttgc tacccacccc tcccagggac aaccactgtc ctgaatttca
     5101 cgataattat tcctttgcct ttcatttctg ttttatcacc tttgtatgta tctttaaaca
     5161 acatataccc ttttttactt atgtaaatgg actgactcat actgcataca tcttctatga
     5221 cttgattctt ttgttcaata ttatatctga gattcatcca tggtgatgca aataggtgca
     5281 ttattttttt tcactgctct gtagtctggc attgtatgaa tacagcacaa tgtatcagtt
     5341 ttaatattgg ggatcattag cattattctc aggtttttaa aaattataag cagtactact
     5401 atggacattc tggtctacat ctcctggcac atatgtaaga gtttctctag ggtataacct
     5461 aggaatggag ggtatgaaca tgtttacatg cacaaactag ctgatgccaa actggtaatg
     5521 ccaactgaaa acattgctgt caatctgatg aatatgaatt gatgtatcaa tgagaatttc
     5581 atttgcattt ccctagtatc tattggggat gaatatattt tcatgtttct ggggcatttg
     5641 catttccact tgttttttaa atattgtgtc tgataattcc aatgtctgga ttctttgttt
     5701 atctgttgtt tctgttggtt tttactcatg gtattttatt tccttgtatg tcttgtgaat
     5761 ttttattata agctcgttct ccttaaaact ttatctgtgg acattc
//
