LOCUS       NM_000443               4293 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens ATP binding cassette subfamily B member 4 (ABCB4),
            transcript variant A, mRNA.
ACCESSION   NM_000443
VERSION     NM_000443.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Cao,L., Ling,X., Yan,J., Feng,D., Dong,Y., Xu,Z., Wang,F., Zhu,S.,
            Gao,Y., Cao,Z. and Zhang,M.
  TITLE     Clinical and genetic study of ABCB4 gene-related cholestatic liver
            disease in China: children and adults
  JOURNAL   Orphanet J Rare Dis 19 (1), 157 (2024)
   PUBMED   38610052
  REMARK    GeneRIF: Clinical and genetic study of ABCB4 gene-related
            cholestatic liver disease in China: children and adults.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Hegarty,R., Gurra,O., Tarawally,J., Allouni,S., Rahman,O.,
            Strautnieks,S., Kyrana,E., Hadzic,N., Thompson,R.J. and
            Grammatikopoulos,T.
  TITLE     Clinical outcomes of ABCB4 heterozygosity in infants and children
            with cholestatic liver disease
  JOURNAL   J Pediatr Gastroenterol Nutr 78 (2), 339-349 (2024)
   PUBMED   38374565
  REMARK    GeneRIF: Clinical outcomes of ABCB4 heterozygosity in infants and
            children with cholestatic liver disease.
REFERENCE   3  (bases 1 to 4293)
  AUTHORS   Zheng,Y., Rao,Q., Han,Y. and He,J.
  TITLE     A novel heterozygous deletion in ABCB4 gene in a Chinese family
            with intrahepatic cholestasis of pregnancy, neonatal
            hyperbilirubinemia, and cholelithiasis: Case reports and literature
            review
  JOURNAL   Mol Genet Genomic Med 12 (1), e2291 (2024)
   PUBMED   37787087
  REMARK    GeneRIF: A novel heterozygous deletion in ABCB4 gene in a Chinese
            family with intrahepatic cholestasis of pregnancy, neonatal
            hyperbilirubinemia, and cholelithiasis: Case reports and literature
            review.
            Review article
REFERENCE   4  (bases 1 to 4293)
  AUTHORS   Thoeni,C., Perciani,C.T., Nakib,D., Camat,D., McGilvray,I.D.,
            MacParland,S.A. and Fischer,S.
  TITLE     Analysis of various ATP-binding cassette transporters revealed
            quantification of ABCB4 as a potential diagnostic tool in primary
            sclerosing cholangitis (PSC)
  JOURNAL   Histopathology 83 (4), 559-568 (2023)
   PUBMED   37488782
  REMARK    GeneRIF: Analysis of various ATP-binding cassette transporters
            revealed quantification of ABCB4 as a potential diagnostic tool in
            primary sclerosing cholangitis (PSC).
REFERENCE   5  (bases 1 to 4293)
  AUTHORS   Smit,J.J., Schinkel,A.H., Mol,C.A., Majoor,D., Mooi,W.J.,
            Jongsma,A.P., Lincke,C.R. and Borst,P.
  TITLE     Tissue distribution of the human MDR3 P-glycoprotein
  JOURNAL   Lab Invest 71 (5), 638-649 (1994)
   PUBMED   7734012
  REMARK    Erratum:[Lab Invest 1995 Mar;72(3):followi]
REFERENCE   6  (bases 1 to 4293)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
REFERENCE   7  (bases 1 to 4293)
  AUTHORS   Lincke,C.R., Smit,J.J., van der Velde-Koerts,T. and Borst,P.
  TITLE     Structure of the human MDR3 gene and physical mapping of the human
            MDR locus
  JOURNAL   J Biol Chem 266 (8), 5303-5310 (1991)
   PUBMED   2002063
REFERENCE   8  (bases 1 to 4293)
  AUTHORS   van der Bliek,A.M., Kooiman,P.M., Schneider,C. and Borst,P.
  TITLE     Sequence of mdr3 cDNA encoding a human P-glycoprotein
  JOURNAL   Gene 71 (2), 401-411 (1988)
   PUBMED   2906314
REFERENCE   9  (bases 1 to 4293)
  AUTHORS   Van der Bliek,A.M., Baas,F., Ten Houte de Lange,T., Kooiman,P.M.,
            Van der Velde-Koerts,T. and Borst,P.
  TITLE     The human mdr3 gene encodes a novel P-glycoprotein homologue and
            gives rise to alternatively spliced mRNAs in liver
  JOURNAL   EMBO J 6 (11), 3325-3331 (1987)
   PUBMED   2892668
REFERENCE   10 (bases 1 to 4293)
  AUTHORS   Callen,D.F., Baker,E., Simmers,R.N., Seshadri,R. and Roninson,I.B.
  TITLE     Localization of the human multiple drug resistance gene, MDR1, to
            7q21.1
  JOURNAL   Hum Genet 77 (2), 142-144 (1987)
   PUBMED   3477522
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005068.2, AC006154.1 and
            AC005045.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_000443.3.
            
            Summary: The membrane-associated protein encoded by this gene is a
            member of the superfamily of ATP-binding cassette (ABC)
            transporters.  ABC proteins transport various molecules across
            extra- and intra-cellular membranes.  ABC genes are divided into
            seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20,
            White).  This protein is a member of the MDR/TAP subfamily.
            Members of the MDR/TAP subfamily are involved in multidrug
            resistance as well as antigen presentation.  This gene encodes a
            full transporter and member of the p-glycoprotein family of
            membrane proteins with phosphatidylcholine as its substrate.  The
            function of this protein has not yet been determined; however, it
            may involve transport of phospholipids from liver hepatocytes into
            bile.  Alternative splicing of this gene results in several
            products of undetermined function. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (A) encodes the predominant
            isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M23234.1, SRR14038194.2284646.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000649586.2/ ENSP00000496956.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                AC005068.2         9573-9619           c
            48-133              AC005068.2         9325-9410           c
            134-188             AC005068.2         6560-6614           c
            189-339             AC006154.1         19676-19826         c
            340-397             AC006154.1         11453-11510         c
            398-589             AC006154.1         9862-10053          c
            590-761             AC006154.1         8541-8712           c
            762-886             AC006154.1         6886-7010           c
            887-1058            AC006154.1         3952-4123           c
            1059-1172           AC006154.1         1780-1893           c
            1173-1283           AC006154.1         581-691             c
            1284-1409           AC006154.1         237-362             c
            1410-1613           AC005045.2         120864-121067       c
            1614-1784           AC005045.2         120332-120502       c
            1785-1946           AC005045.2         112069-112230       c
            1947-2117           AC005045.2         107415-107585       c
            2118-2264           AC005045.2         104571-104717       c
            2265-2369           AC005045.2         102786-102890       c
            2370-2447           AC005045.2         100663-100740       c
            2448-2531           AC005045.2         99202-99285         c
            2532-2735           AC005045.2         97977-98180         c
            2736-2836           AC005045.2         94282-94382         c
            2837-2977           AC005045.2         92558-92698         c
            2978-3134           AC005045.2         89901-90057         c
            3135-3332           AC005045.2         88702-88899         c
            3333-3539           AC005045.2         86953-87159         c
            3540-3686           AC005045.2         83800-83946         c
            3687-3944           AC005045.2         82710-82967         c
            3945-4293           AC005045.2         82361-82709         c
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q21.12"
     gene            1..4293
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /note="ATP binding cassette subfamily B member 4"
                     /db_xref="GeneID:5244"
                     /db_xref="HGNC:HGNC:45"
                     /db_xref="MIM:171060"
     exon            1..47
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            48..133
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     CDS             54..3893
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /EC_number="7.6.2.1"
                     /note="isoform A is encoded by transcript variant A;
                     multiple drug resistance 3; P-glycoprotein-3/multiple drug
                     resistance-3; multidrug resistance protein 3;
                     P-glycoprotein 3; ATP-binding cassette sub-family B member
                     4; phosphatidylcholine translocator ABCB4; ATP-binding
                     cassette, sub-family B (MDR/TAP), member 4"
                     /codon_start=1
                     /product="phosphatidylcholine translocator ABCB4 isoform
                     A"
                     /protein_id="NP_000434.1"
                     /db_xref="CCDS:CCDS5605.1"
                     /db_xref="GeneID:5244"
                     /db_xref="HGNC:HGNC:45"
                     /db_xref="MIM:171060"
                     /translation="MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLT
                     LFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSL
                     LNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQ
                     EIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTL
                     VIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKEL
                     ERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAM
                     TVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKG
                     NLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDE
                     GTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKE
                     ANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTE
                     SEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVY
                     FKLVNMQTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLD
                     VETDGLEANVPPVSFLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFG
                     PGDDAVKQQKCNIFSLIFLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQ
                     DMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQL
                     TLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERK
                     FESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRD
                     VILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKF
                     EGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDP
                     LAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVS
                     AAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSA
                     LDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQ
                     KGIYFSMVSVQAGTQNL"
     exon            134..188
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            189..339
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            340..397
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            398..589
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            590..761
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            762..886
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1058
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1172
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1283
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1409
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1613
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1784
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1785..1946
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2117
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2264
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2369
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2447
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2531
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2735
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2736..2836
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..2977
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3134
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3332
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3539
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            3540..3686
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     exon            3687..4293
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3918..3923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /note="hexamer: AATAAA"
     polyA_site      3945
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /note="major polyA site"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
                     /note="hexamer: AATAAA"
     polyA_site      4293
                     /gene="ABCB4"
                     /gene_synonym="ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3;
                     PFIC-3; PGY3"
ORIGIN      
        1 agcgcccgcg cgtccagagg ccctgccaga cacgcgcgag gttcgaggct gagatggatc
       61 ttgaggcggc aaagaacgga acagcctggc gccccacgag cgcggagggc gactttgaac
      121 tgggcatcag cagcaaacaa aaaaggaaaa aaacgaagac agtgaaaatg attggagtat
      181 taacattgtt tcgatactcc gattggcagg ataaattgtt tatgtcgctg ggtaccatca
      241 tggccatagc tcacggatca ggtctccccc tcatgatgat agtatttgga gagatgactg
      301 acaaatttgt tgatactgca ggaaacttct cctttccagt gaacttttcc ttgtcgctgc
      361 taaatccagg caaaattctg gaagaagaaa tgactagata tgcatattac tactcaggat
      421 tgggtgctgg agttcttgtt gctgcctata tacaagtttc attttggact ttggcagctg
      481 gtcgacagat caggaaaatt aggcagaagt tttttcatgc tattctacga caggaaatag
      541 gatggtttga catcaacgac accactgaac tcaatacgcg gctaacagat gacatctcca
      601 aaatcagtga aggaattggt gacaaggttg gaatgttctt tcaagcagta gccacgtttt
      661 ttgcaggatt catagtggga ttcatcagag gatggaagct cacccttgtg ataatggcca
      721 tcagccctat tctaggactc tctgcagccg tttgggcaaa gatactctcg gcatttagtg
      781 acaaagaact agctgcttat gcaaaagcag gcgccgtggc agaagaggct ctgggggcca
      841 tcaggactgt gatagctttc gggggccaga acaaagagct ggaaaggtat cagaaacatt
      901 tagaaaatgc caaagagatt ggaattaaaa aagctatttc agcaaacatt tccatgggta
      961 ttgccttcct gttaatatat gcatcatatg cactggcctt ctggtatgga tccactctag
     1021 tcatatcaaa agaatatact attggaaatg caatgacagt ttttttttca atcctaattg
     1081 gagctttcag tgttggccag gctgccccat gtattgatgc ttttgccaat gcaagaggag
     1141 cagcatatgt gatctttgat attattgata ataatcctaa aattgacagt ttttcagaga
     1201 gaggacacaa accagacagc atcaaaggga atttggagtt caatgatgtt cacttttctt
     1261 acccttctcg agctaacgtc aagatcttga agggcctcaa cctgaaggtg cagagtgggc
     1321 agacggtggc cctggttgga agtagtggct gtgggaagag cacaacggtc cagctgatac
     1381 agaggctcta tgaccctgat gagggcacaa ttaacattga tgggcaggat attaggaact
     1441 ttaatgtaaa ctatctgagg gaaatcattg gtgtggtgag tcaggagccg gtgctgtttt
     1501 ccaccacaat tgctgaaaat atttgttatg gccgtggaaa tgtaaccatg gatgagataa
     1561 agaaagctgt caaagaggcc aacgcctatg agtttatcat gaaattacca cagaaatttg
     1621 acaccctggt tggagagaga ggggcccagc tgagtggtgg gcagaagcag aggatcgcca
     1681 ttgcacgtgc cctggttcgc aaccccaaga tccttctgct ggatgaggcc acgtcagcat
     1741 tggacacaga aagtgaagct gaggtacagg cagctctgga taaggccaga gaaggccgga
     1801 ccaccattgt gatagcacac cgactgtcta cggtccgaaa tgcagatgtc atcgctgggt
     1861 ttgaggatgg agtaattgtg gagcaaggaa gccacagcga actgatgaag aaggaagggg
     1921 tgtacttcaa acttgtcaac atgcagacat caggaagcca gatccagtca gaagaatttg
     1981 aactaaatga tgaaaaggct gccactagaa tggccccaaa tggctggaaa tctcgcctat
     2041 ttaggcattc tactcagaaa aaccttaaaa attcacaaat gtgtcagaag agccttgatg
     2101 tggaaaccga tggacttgaa gcaaatgtgc caccagtgtc ctttctgaag gtcctgaaac
     2161 tgaataaaac agaatggccc tactttgtcg tgggaacagt atgtgccatt gccaatgggg
     2221 ggcttcagcc ggcattttca gtcatattct cagagatcat agcgattttt ggaccaggcg
     2281 atgatgcagt gaagcagcag aagtgcaaca tattctcttt gattttctta tttctgggaa
     2341 ttatttcttt ttttactttc ttccttcagg gtttcacgtt tgggaaagct ggcgagatcc
     2401 tcaccagaag actgcggtca atggctttta aagcaatgct aagacaggac atgagctggt
     2461 ttgatgacca taaaaacagt actggtgcac tttctacaag acttgccaca gatgctgccc
     2521 aagtccaagg agccacagga accaggttgg ctttaattgc acagaatata gctaaccttg
     2581 gaactggtat tatcatatca tttatctacg gttggcagtt aaccctattg ctattagcag
     2641 ttgttccaat tattgctgtg tcaggaattg ttgaaatgaa attgttggct ggaaatgcca
     2701 aaagagataa aaaagaactg gaagctgctg gaaagattgc aacagaggca atagaaaata
     2761 ttaggacagt tgtgtctttg acccaggaaa gaaaatttga atcaatgtat gttgaaaaat
     2821 tgtatggacc ttacaggaat tctgtgcaga aggcacacat ctatggaatt acttttagta
     2881 tctcacaagc atttatgtat ttttcctatg ccggttgttt tcgatttggt gcatatctca
     2941 ttgtgaatgg acatatgcgc ttcagagatg ttattctggt gttttctgca attgtatttg
     3001 gtgcagtggc tctaggacat gccagttcat ttgctccaga ctatgctaaa gctaagctgt
     3061 ctgcagccca cttattcatg ctgtttgaaa gacaacctct gattgacagc tacagtgaag
     3121 aggggctgaa gcctgataaa tttgaaggaa atataacatt taatgaagtc gtgttcaact
     3181 atcccacccg agcaaacgtg ccagtgcttc aggggctgag cctggaggtg aagaaaggcc
     3241 agacactagc cctggtgggc agcagtggct gtgggaagag cacggtggtc cagctcctgg
     3301 agcggttcta cgaccccttg gcggggacag tgcttctcga tggtcaagaa gcaaagaaac
     3361 tcaatgtcca gtggctcaga gctcaactcg gaatcgtgtc tcaggagcct atcctatttg
     3421 actgcagcat tgccgagaat attgcctatg gagacaacag ccgggttgta tcacaggatg
     3481 aaattgtgag tgcagccaaa gctgccaaca tacatccttt catcgagacg ttaccccaca
     3541 aatatgaaac aagagtggga gataagggga ctcagctctc aggaggtcaa aaacagagga
     3601 ttgctattgc ccgagccctc atcagacaac ctcaaatcct cctgttggat gaagctacat
     3661 cagctctgga tactgaaagt gaaaaggttg tccaagaagc cctggacaaa gccagagaag
     3721 gccgcacctg cattgtgatt gctcaccgcc tgtccaccat ccagaatgca gacttaatag
     3781 tggtgtttca gaatgggaga gtcaaggagc atggcacgca tcagcagctg ctggcacaga
     3841 aaggcatcta tttttcaatg gtcagtgtcc aggctgggac acagaactta tgaacttttg
     3901 ctacagtata ttttaaaaat aaattcaaat tattctacca ttttatctga cttgtaaaat
     3961 aagtttctgg tttgtcatca taaaacatag gtatgtctgt ctcattctag atcaatttag
     4021 aagacccaat ttggggttta ttacggcatt tgaaatttga gagttagaag tgacacctgt
     4081 cttgatgaag tattgaaaca acaggctagc tctccctaag ttattagaaa catccagaaa
     4141 aagaacaaaa ttcaaggtaa gtacaggtgt agaaatatag aagccacaga catagggaaa
     4201 tggtcttatg gtcctgaatg ggttgggtag acaggaaagt ttgggaataa ttttcccaag
     4261 tacaaaagaa aaataaattg ttcaatagcc tca
//
