LOCUS       NM_003742               6934 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens ATP binding cassette subfamily B member 11 (ABCB11),
            mRNA.
ACCESSION   NM_003742
VERSION     NM_003742.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Khabou,B., Kallabi,F., Abdelaziz,R.B., Maaloul,I., Aloulou,H.,
            Chehida,A.B., Kammoun,T., Barbu,V., Boudawara,T.S., Fakhfakh,F.,
            Khemakhem,B. and Sahnoun,O.S.
  TITLE     Molecular and computational characterization of ABCB11 and ABCG5
            variants in Tunisian patients with neonatal/infantile low-GGT
            intrahepatic cholestasis: Genetic diagnosis and genotype-phenotype
            correlation assessment
  JOURNAL   Ann Hum Genet 88 (3), 194-211 (2024)
   PUBMED   38108658
  REMARK    GeneRIF: Molecular and computational characterization of ABCB11 and
            ABCG5 variants in Tunisian patients with neonatal/infantile low-GGT
            intrahepatic cholestasis: Genetic diagnosis and genotype-phenotype
            correlation assessment.
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Pang,L., Cui,M., Wu,S. and Kong,J.
  TITLE     Cav-1 regulates the bile salt export pump on the canalicular
            membrane of hepatocytes by PKCalpha-associated signalling under
            cholesterol stimulation
  JOURNAL   J Cell Mol Med 28 (3), e18110 (2024)
   PUBMED   38164042
  REMARK    GeneRIF: Cav-1 regulates the bile salt export pump on the
            canalicular membrane of hepatocytes by PKCalpha-associated
            signalling under cholesterol stimulation.
REFERENCE   3  (bases 1 to 6934)
  AUTHORS   Liu,H., Irobalieva,R.N., Kowal,J., Ni,D., Nosol,K.,
            Bang-Sorensen,R., Lancien,L., Stahlberg,H., Stieger,B. and
            Locher,K.P.
  TITLE     Structural basis of bile salt extrusion and small-molecule
            inhibition in human BSEP
  JOURNAL   Nat Commun 14 (1), 7296 (2023)
   PUBMED   37949847
  REMARK    GeneRIF: Structural basis of bile salt extrusion and small-molecule
            inhibition in human BSEP.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6934)
  AUTHORS   Pfister,E.D., Jaeger,V.K., Karch,A., Shay,D., Schukfeh,N.,
            Ohlendorf,J., Junge,N., Goldschmidt,I., Stalke,A.,
            Keitel-Anselmino,V. and Baumann,U.
  TITLE     Native liver survival in bile salt export pump deficiency: results
            of a retrospective cohort study
  JOURNAL   Hepatol Commun 7 (4), e0092 (2023)
   PUBMED   36995996
  REMARK    GeneRIF: Native liver survival in bile salt export pump deficiency:
            results of a retrospective cohort study.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6934)
  AUTHORS   Wang,R., Salem,M., Yousef,I.M., Tuchweber,B., Lam,P., Childs,S.J.,
            Helgason,C.D., Ackerley,C., Phillips,M.J. and Ling,V.
  TITLE     Targeted inactivation of sister of P-glycoprotein gene (spgp) in
            mice results in nonprogressive but persistent intrahepatic
            cholestasis
  JOURNAL   Proc Natl Acad Sci U S A 98 (4), 2011-2016 (2001)
   PUBMED   11172067
REFERENCE   6  (bases 1 to 6934)
  AUTHORS   Jansen,P.L., Strautnieks,S.S., Jacquemin,E., Hadchouel,M.,
            Sokal,E.M., Hooiveld,G.J., Koning,J.H., De Jager-Krikken,A.,
            Kuipers,F., Stellaard,F., Bijleveld,C.M., Gouw,A., Van Goor,H.,
            Thompson,R.J. and Muller,M.
  TITLE     Hepatocanalicular bile salt export pump deficiency in patients with
            progressive familial intrahepatic cholestasis
  JOURNAL   Gastroenterology 117 (6), 1370-1379 (1999)
   PUBMED   10579978
REFERENCE   7  (bases 1 to 6934)
  AUTHORS   Strautnieks,S.S., Bull,L.N., Knisely,A.S., Kocoshis,S.A., Dahl,N.,
            Arnell,H., Sokal,E., Dahan,K., Childs,S., Ling,V., Tanner,M.S.,
            Kagalwalla,A.F., Nemeth,A., Pawlowska,J., Baker,A.,
            Mieli-Vergani,G., Freimer,N.B., Gardiner,R.M. and Thompson,R.J.
  TITLE     A gene encoding a liver-specific ABC transporter is mutated in
            progressive familial intrahepatic cholestasis
  JOURNAL   Nat Genet 20 (3), 233-238 (1998)
   PUBMED   9806540
REFERENCE   8  (bases 1 to 6934)
  AUTHORS   Gerloff,T., Stieger,B., Hagenbuch,B., Madon,J., Landmann,L.,
            Roth,J., Hofmann,A.F. and Meier,P.J.
  TITLE     The sister of P-glycoprotein represents the canalicular bile salt
            export pump of mammalian liver
  JOURNAL   J Biol Chem 273 (16), 10046-10050 (1998)
   PUBMED   9545351
REFERENCE   9  (bases 1 to 6934)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
REFERENCE   10 (bases 1 to 6934)
  AUTHORS   Bull,L.N., Morotti,R. and Squires,J.E.
  TITLE     ATP8B1 Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301474
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC008177.3, AF091582.1,
            AC069137.6 and CB161524.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 8, 2018 this sequence version replaced NM_003742.3.
            
            Summary: The membrane-associated protein encoded by this gene is a
            member of the superfamily of ATP-binding cassette (ABC)
            transporters. ABC proteins transport various molecules across
            extra- and intra-cellular membranes. ABC genes are divided into
            seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20,
            White). This protein is a member of the MDR/TAP subfamily. Members
            of the MDR/TAP subfamily are involved in multidrug resistance. The
            protein encoded by this gene is the major canalicular bile salt
            export pump in man. Mutations in this gene cause a form of
            progressive familial intrahepatic cholestases which are a group of
            inherited disorders with severe cholestatic liver disease from
            early infancy. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF091582.1, AF136523.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000650372.1/ ENSP00000497931.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC008177.3         92136-92136         c
            2-1141              AF091582.1         1-1140
            1142-1142           AC008177.3         46990-46990         c
            1143-4169           AF091582.1         1142-4168
            4170-6928           AC069137.6         90425-93183         c
            6929-6934           CB161524.1         17-22               c
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..6934
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /note="ATP binding cassette subfamily B member 11"
                     /db_xref="GeneID:8647"
                     /db_xref="HGNC:HGNC:42"
                     /db_xref="MIM:603201"
     exon            1..100
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            101..203
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..4093
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /note="progressive familial intrahepatic cholestasis 2;
                     ABC member 16, MDR/TAP subfamily; sister p-glycoprotein;
                     ATP-binding cassette sub-family B member 11; ATP-binding
                     cassette, sub-family B (MDR/TAP), member 11"
                     /codon_start=1
                     /product="bile salt export pump"
                     /protein_id="NP_003733.2"
                     /db_xref="CCDS:CCDS46444.1"
                     /db_xref="GeneID:8647"
                     /db_xref="HGNC:HGNC:42"
                     /db_xref="MIM:603201"
                     /translation="MSDSVILRSIKKFGEENDGFESDKSYNNDKKSRLQDEKKGDGVR
                     VGFFQLFRFSSSTDIWLMFVGSLCAFLHGIAQPGVLLIFGTMTDVFIDYDVELQELQI
                     PGKACVNNTIVWTNSSLNQNMTNGTRCGLLNIESEMIKFASYYAGIAVAVLITGYIQI
                     CFWVIAAARQIQKMRKFYFRRIMRMEIGWFDCNSVGELNTRFSDDINKINDAIADQMA
                     LFIQRMTSTICGFLLGFFRGWKLTLVIISVSPLIGIGAATIGLSVSKFTDYELKAYAK
                     AGVVADEVISSMRTVAAFGGEKREVERYEKNLVFAQRWGIRKGIVMGFFTGFVWCLIF
                     LCYALAFWYGSTLVLDEGEYTPGTLVQIFLSVIVGALNLGNASPCLEAFATGRAAATS
                     IFETIDRKPIIDCMSEDGYKLDRIKGEIEFHNVTFHYPSRPEVKILNDLNMVIKPGEM
                     TALVGPSGAGKSTALQLIQRFYDPCEGMVTVDGHDIRSLNIQWLRDQIGIVEQEPVLF
                     STTIAENIRYGREDATMEDIVQAAKEANAYNFIMDLPQQFDTLVGEGGGQMSGGQKQR
                     VAIARALIRNPKILLLDMATSALDNESEAMVQEVLSKIQHGHTIISVAHRLSTVRAAD
                     TIIGFEHGTAVERGTHEELLERKGVYFTLVTLQSQGNQALNEEDIKDATEDDMLARTF
                     SRGSYQDSLRASIRQRSKSQLSYLVHEPPLAVVDHKSTYEEDRKDKDIPVQEEVEPAP
                     VRRILKFSAPEWPYMLVGSVGAAVNGTVTPLYAFLFSQILGTFSIPDKEEQRSQINGV
                     CLLFVAMGCVSLFTQFLQGYAFAKSGELLTKRLRKFGFRAMLGQDIAWFDDLRNSPGA
                     LTTRLATDASQVQGAAGSQIGMIVNSFTNVTVAMIIAFSFSWKLSLVILCFFPFLALS
                     GATQTRMLTGFASRDKQALEMVGQITNEALSNIRTVAGIGKERRFIEALETELEKPFK
                     TAIQKANIYGFCFAFAQCIMFIANSASYRYGGYLISNEGLHFSYVFRVISAVVLSATA
                     LGRAFSYTPSYAKAKISAARFFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYP
                     SRPDSQVLNGLSVSISPGQTLAFVGSSGCGKSTSIQLLERFYDPDQGKVMIDGHDSKK
                     VNVQFLRSNIGIVSQEPVLFACSIMDNIKYGDNTKEIPMERVIAAAKQAQLHDFVMSL
                     PEKYETNVGSQGSQLSRGEKQRIAIARAIVRDPKILLLDEATSALDTESEKTVQVALD
                     KAREGRTCIVIAHRLSTIQNADIIAVMAQGVVIEKGTHEELMAQKGAYYKLVTTGSPI
                     S"
     exon            204..225
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            226..277
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            278..516
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            517..604
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            605..738
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            739..910
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1035
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1210
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1324
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1435
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1561
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1765
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1936
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2138
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2139..2202
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2305
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2470
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2575
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2737
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2738..2941
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            2942..3183
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3340
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            3341..3538
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3745
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            3746..3892
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     exon            3893..6934
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6207..6212
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
                     /note="hexamer: AATAAA"
     polyA_site      6229
                     /gene="ABCB11"
                     /gene_synonym="ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4;
                     SPGP"
ORIGIN      
        1 agaatgatga aaaccgaggt tggaaaaggt tgtgaaacct tttaactctc cacagtggag
       61 tccattattt cctctggctt cctcaaattc atattcacag ggtcgttggc tgtgggttgc
      121 aattaccatg tctgactcag taattcttcg aagtataaag aaatttggag aggagaatga
      181 tggttttgag tcagataaat catataataa tgataagaaa tcaaggttac aagatgagaa
      241 gaaaggtgat ggcgttagag ttggcttctt tcaattgttt cggttttctt catcaactga
      301 catttggctg atgtttgtgg gaagtttgtg tgcatttctc catggaatag cccagccagg
      361 cgtgctactc atttttggca caatgacaga tgtttttatt gactacgacg ttgagttaca
      421 agaactccag attccaggaa aagcatgtgt gaataacacc attgtatgga ctaacagttc
      481 cctcaaccag aacatgacaa atggaacacg ttgtgggttg ctgaacatcg agagcgaaat
      541 gatcaaattt gccagttact atgctggaat tgctgtcgca gtacttatca caggatatat
      601 tcaaatatgc ttttgggtca ttgccgcagc tcgtcagata cagaaaatga gaaaatttta
      661 ctttaggaga ataatgagaa tggaaatagg gtggtttgac tgcaattcag tgggggagct
      721 gaatacaaga ttctctgatg atattaataa aatcaatgat gccatagctg accaaatggc
      781 ccttttcatt cagcgcatga cctcgaccat ctgtggtttc ctgttgggat ttttcagggg
      841 ttggaaactg accttggtta ttatttctgt cagccctctc attgggattg gagcagccac
      901 cattggtctg agtgtgtcca agtttacgga ctatgagctg aaggcctatg ccaaagcagg
      961 ggtggtggct gatgaagtca tttcatcaat gagaacagtg gctgcttttg gtggtgagaa
     1021 aagagaggtt gaaaggtatg agaaaaatct tgtgttcgcc cagcgttggg gaattagaaa
     1081 aggaatagtg atgggattct ttactggatt cgtgtggtgt ctcatctttt tgtgttatgc
     1141 actggccttc tggtacggct ccacacttgt cctggatgaa ggagaatata caccaggaac
     1201 ccttgtccag attttcctca gtgtcatagt aggagcttta aatcttggca atgcctctcc
     1261 ttgtttggaa gcctttgcaa ctggacgtgc agcagccacc agcatttttg agacaataga
     1321 caggaaaccc atcattgact gcatgtcaga agatggttac aagttggatc gaatcaaggg
     1381 tgaaattgaa ttccataatg tgaccttcca ttatccttcc agaccagagg tgaagattct
     1441 aaatgacctc aacatggtca ttaaaccagg ggaaatgaca gctctggtag gacccagtgg
     1501 agctggaaaa agtacagcac tgcaactcat tcagcgattc tatgacccct gtgaaggaat
     1561 ggtgaccgtg gatggccatg acattcgctc tcttaacatt cagtggctta gagatcagat
     1621 tgggatagtg gagcaagagc cagttctgtt ctctaccacc attgcagaaa atattcgcta
     1681 tggcagagaa gatgcaacaa tggaagacat agtccaagct gccaaggagg ccaatgccta
     1741 caacttcatc atggacctgc cacagcaatt tgacaccctt gttggagaag gaggaggcca
     1801 gatgagtggt ggccagaaac aaagggtagc tatcgccaga gccctcatcc gaaatcccaa
     1861 gattctgctt ttggacatgg ccacctcagc tctggacaat gagagtgaag ccatggtgca
     1921 agaagtgctg agtaagattc agcatgggca cacaatcatt tcagttgctc atcgcttgtc
     1981 tacggtcaga gctgcagata ccatcattgg ttttgaacat ggcactgcag tggaaagagg
     2041 gacccatgaa gaattactgg aaaggaaagg tgtttacttc actctagtga ctttgcaaag
     2101 ccagggaaat caagctctta atgaagagga cataaaggat gcaactgaag atgacatgct
     2161 tgcgaggacc tttagcagag ggagctacca ggatagttta agggcttcca tccggcaacg
     2221 ctccaagtct cagctttctt acctggtgca cgaacctcca ttagctgttg tagatcataa
     2281 gtctacctat gaagaagata gaaaggacaa ggacattcct gtgcaggaag aagttgaacc
     2341 tgccccagtt aggaggattc tgaaattcag tgctccagaa tggccctaca tgctggtagg
     2401 gtctgtgggt gcagctgtga acgggacagt cacacccttg tatgcctttt tattcagcca
     2461 gattcttggg actttttcaa ttcctgataa agaggaacaa aggtcacaga tcaatggtgt
     2521 gtgcctactt tttgtagcaa tgggctgtgt atctcttttc acccaatttc tacagggata
     2581 tgcctttgct aaatctgggg agctcctaac aaaaaggcta cgtaaatttg gtttcagggc
     2641 aatgctgggg caagatattg cctggtttga tgacctcaga aatagccctg gagcattgac
     2701 aacaagactt gctacagatg cttcccaagt tcaaggggct gccggctctc agatcgggat
     2761 gatagtcaat tccttcacta acgtcactgt ggccatgatc attgccttct cctttagctg
     2821 gaagctgagc ctggtcatct tgtgcttctt ccccttcttg gctttatcag gagccacaca
     2881 gaccaggatg ttgacaggat ttgcctctcg agataagcag gccctggaga tggtgggaca
     2941 gattacaaat gaagccctca gtaacatccg cactgttgct ggaattggaa aggagaggcg
     3001 gttcattgaa gcacttgaga ctgagctgga gaagcccttc aagacagcca ttcagaaagc
     3061 caatatttac ggattctgct ttgcctttgc ccagtgcatc atgtttattg cgaattctgc
     3121 ttcctacaga tatggaggtt acttaatctc caatgagggg ctccatttca gctatgtgtt
     3181 cagggtgatc tctgcagttg tactgagtgc aacagctctt ggaagagcct tctcttacac
     3241 cccaagttat gcaaaagcta aaatatcagc tgcacgcttt tttcaactgc tggaccgaca
     3301 acccccaatc agtgtataca atactgcagg tgaaaaatgg gacaacttcc aggggaagat
     3361 tgattttgtt gattgtaaat ttacatatcc ttctcgacct gactcgcaag ttctgaatgg
     3421 tctctcagtg tcgattagtc cagggcagac actggcgttt gttgggagca gtggatgtgg
     3481 caaaagcact agcattcagc tgttggaacg tttctatgat cctgatcaag ggaaggtgat
     3541 gatagatggt catgacagca aaaaagtaaa tgtccagttc ctccgctcaa acattggaat
     3601 tgtttcccag gaaccagtgt tgtttgcctg tagcataatg gacaatatca agtatggaga
     3661 caacaccaaa gaaattccca tggaaagagt catagcagct gcaaaacagg ctcagctgca
     3721 tgattttgtc atgtcactcc cagagaaata tgaaactaac gttgggtccc aggggtctca
     3781 actctctaga ggggagaaac aacgcattgc tattgctcgg gccattgtac gagatcctaa
     3841 aatcttgcta ctagatgaag ccacttctgc cttagacaca gaaagtgaaa agacggtgca
     3901 ggttgctcta gacaaagcca gagagggtcg gacctgcatt gtcattgccc atcgcttgtc
     3961 caccatccag aacgcggata tcattgctgt catggcacag ggggtggtga ttgaaaaggg
     4021 gacccatgaa gaactgatgg cccaaaaagg agcctactac aaactagtca ccactggatc
     4081 ccccatcagt tgacccaatg caagaatctc agacacacat gacgcaccag ttacaggggt
     4141 tgtttttaaa gaaaaaaaca atcccagcag gagggattgc tgggattgtt ttttctttaa
     4201 agaagaatgt taatatttta cttttacagt cattttccta catcggaatc caagctaatt
     4261 tctaatggcc ttccataata attctgcttt agatgtgtat acagaaaatg aaagaaacta
     4321 gggtccatat gagggaaaac ccaatgtcaa gtggcagctc agccaccact cagtgcttct
     4381 ctgtgcagga gccagtcctg attaatatgt gggaattagt gagacatcag ggagtaagtg
     4441 acactttgaa ctcctcaagg gcagagaact gtctttcatt tttgaaccct cggtgtacac
     4501 agaggcgggt ctataacagg caatcaacaa acgtttcttg agctagacca aggtcagatt
     4561 tgaaaagaac agaaggactg aagaccagct gtgtttctta actaaatttg tctttcaagt
     4621 gaaaccagct tccttcatct ctaaggctaa ggatagggaa agggtggatg ctctcaggct
     4681 gagggaggca gaaagggaaa gtattagcat gagctttcca gttagggctg ttgatttatg
     4741 ctttaacttc agagtgagtg taggggtggt gatgctacca ttactgtgag gacctaccag
     4801 tgtggctgga gcagggactc tctcccaggc cttttactcc tcagcacctc cctgcatact
     4861 gattgttgtt tttagtttct gtgaaattat attcatgaaa tgaaaatagc gcattttact
     4921 ttgctgtagt ttcataaggt tttatacaaa aaagcaagta aatatggcag aaaagcactc
     4981 atttgcccct gctccctcaa aacaccacag aatgacatag aactaaaggc ggcaggaatc
     5041 tacaagaatg aagaaaacac agtgatgcca cctgcaaaat cttgggagcc agaaagcaaa
     5101 tggacaattg ataatagagt tacaagatga gagaaaacaa aaatgtaacc tgttagttgg
     5161 gggagcctag aaacatcctg ttttgtacca cagaccccta gaaagtttca agatgtaaaa
     5221 acactggatc cttctggaag gaggagacaa ggggacagag ggactgaaga cagaagaatg
     5281 gaccaaaagc ctgtatggaa acagaattgc agagccccgc ctgcagcagg atggctgcct
     5341 ttccccagtt ccagcgagaa actgcatact ccctctctga ggaggctcac agggaggttt
     5401 gcacttagag acacaagatg aagttgaagg ggcagctaac atacaagaga cagggggatt
     5461 gaatgtaaag ctgtgtatga acagaagaac tcccagcccc tctcttctgg caggagattg
     5521 aagaatgatt ttctggggaa attggccaat cttagaaaag aggtcgggcc aggcgctgtg
     5581 gctcactcct gtaatcccag cactttggga ggccaaggcg ggcggatcat gaggtcgaga
     5641 gattgagacc atcctggcta acatggtgaa accccgtctc tactaaaaat acaaaaaaat
     5701 tagccgggcg tggtggcggg cgcctgtagt cccagctact caggaggctg aggcaggaga
     5761 atggcgtgaa cccgggaggt ggagcttgca gtgagccgag atcgcgccac tgcactccag
     5821 cctgggcgac agagcgaaaa aaaaaaaaaa agaaaaagaa aaagaaaaga ggtcaaggac
     5881 tccgtccttg gaatcctaag aaaattttcc agccgtatta cccttctatg aagcccacct
     5941 gtcaaccaac aagcacccac tcgatcagag cttccccagg ctttttggtg tctcctcctt
     6001 gcatgggaat tgacttccaa ggaccaccag acactgagga agtattttaa catataaagc
     6061 aaaagcaaca atagggcagc tggagaaagg aaattagaag taacagagcc aatgcagtga
     6121 ttagaaaaac actcaaaaaa tggtaataaa tgtgttcaat gggtcaagag aacatatttc
     6181 catctattta aataaaaaca ggaatcaata aaagtgaaca tacaaaaaag agctcttgtc
     6241 acagaaatga aaaccttggg agcaggactg cacccacggt tcccaccaac ctatcctgcc
     6301 atcatgtttt ctccctactc cagcttcctc ccaagctcac tctacgtaga tggtttttct
     6361 tcccttagcc aacaaacgaa attctgccta taaattaagc caggacacta ggatatggag
     6421 gtatattatt tatgtctctt ttccagtgtt tcctggtata taacagcaaa gatggtttag
     6481 caagttaact ggtcaggcca gaattcttga tggttgccaa gtcaggaggc agacggtgaa
     6541 ggaatgcatt ctggttatga aaaatggcaa tgacgagtaa gttgcagagt aaagatgcct
     6601 tttagaggaa attaaggttt gcaataaatg catgctaatc ctctaatttt gatgaaagac
     6661 taaagctctt gttttggttt gcatttcaaa gagcaagagc ataggctctt gtgctgggag
     6721 gtggccagtt tcctggaaag gaatttatgg agtctgaaag aggaattagt acaaattata
     6781 ttttcaaata agatacttga aagttattga tgacagggaa aagattgtct aaagagggct
     6841 tgaaattggg gaaataattg aggctcagtg acaaaggaga agattacaac ttcaaaaatg
     6901 aataaataaa taacattgca tgtttatttt acaa
//
