LOCUS       NM_020745               4798 bp    mRNA    linear   PRI 16-FEB-2025
DEFINITION  Homo sapiens alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_020745 XM_291190
VERSION     NM_020745.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Li,H., Liu,C., Li,R., Zhou,L., Ran,Y., Yang,Q., Huang,H., Lu,H.,
            Song,H., Yang,B., Ru,H., Lin,S. and Zhang,L.
  TITLE     AARS1 and AARS2 sense L-lactate to regulate cGAS as global lysine
            lactyltransferases
  JOURNAL   Nature 634 (8036), 1229-1237 (2024)
   PUBMED   39322678
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Liu,L., Gao,J., Liu,X., Zhang,F., Hu,B., Zhang,H., Wang,Z.,
            Tang,H., Shi,J.H. and Zhang,S.
  TITLE     AARS2 as a novel biomarker for prognosis and its molecular
            characterization in pan-cancer
  JOURNAL   Cancer Med 12 (23), 21531-21544 (2023)
   PUBMED   37990642
  REMARK    GeneRIF: AARS2 as a novel biomarker for prognosis and its molecular
            characterization in pan-cancer.
REFERENCE   3  (bases 1 to 4798)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 4798)
  AUTHORS   Roux,C.J., Barcia,G., Schiff,M., Sissler,M., Levy,R.,
            Dangouloff-Ros,V., Desguerre,I., Edvardson,S., Elpeleg,O.,
            Rotig,A., Munnich,A. and Boddaert,N.
  TITLE     Phenotypic diversity of brain MRI patterns in mitochondrial
            aminoacyl-tRNA synthetase mutations
  JOURNAL   Mol Genet Metab 133 (2), 222-229 (2021)
   PUBMED   33972171
  REMARK    GeneRIF: Phenotypic diversity of brain MRI patterns in
            mitochondrial aminoacyl-tRNA synthetase mutations.
REFERENCE   5  (bases 1 to 4798)
  AUTHORS   Hamatani,M., Jingami,N., Tsurusaki,Y., Shimada,S., Shimojima,K.,
            Asada-Utsugi,M., Yoshinaga,K., Uemura,N., Yamashita,H., Uemura,K.,
            Takahashi,R., Matsumoto,N. and Yamamoto,T.
  TITLE     The first Japanese case of leukodystrophy with ovarian failure
            arising from novel compound heterozygous AARS2 mutations
  JOURNAL   J Hum Genet 61 (10), 899-902 (2016)
   PUBMED   27251004
  REMARK    GeneRIF: we describe a Japanese woman with novel compound
            heterozygous mutations in AARS2, the first report of leukodystrophy
            caused by AARS2 mutations in Asia.
REFERENCE   6  (bases 1 to 4798)
  AUTHORS   Dallabona,C., Diodato,D., Kevelam,S.H., Haack,T.B., Wong,L.J.,
            Salomons,G.S., Baruffini,E., Melchionda,L., Mariotti,C.,
            Strom,T.M., Meitinger,T., Prokisch,H., Chapman,K., Colley,A.,
            Rocha,H., Ounap,K., Schiffmann,R., Salsano,E., Savoiardo,M.,
            Hamilton,E.M., Abbink,T.E., Wolf,N.I., Ferrero,I., Lamperti,C.,
            Zeviani,M., Vanderver,A., Ghezzi,D. and van der Knaap,M.S.
  TITLE     Novel (ovario) leukodystrophy related to AARS2 mutations
  JOURNAL   Neurology 82 (23), 2063-2071 (2014)
   PUBMED   24808023
  REMARK    GeneRIF: A new phenotype caused by AARS2 mutations is characterized
            by leukoencephalopathy and ovarian failure in female patients,
            indicating that the phenotypic spectrum associated with AARS2
            variants is much wider than previously reported.
REFERENCE   7  (bases 1 to 4798)
  AUTHORS   Calvo,S.E., Compton,A.G., Hershman,S.G., Lim,S.C., Lieber,D.S.,
            Tucker,E.J., Laskowski,A., Garone,C., Liu,S., Jaffe,D.B.,
            Christodoulou,J., Fletcher,J.M., Bruno,D.L., Goldblatt,J.,
            Dimauro,S., Thorburn,D.R. and Mootha,V.K.
  TITLE     Molecular diagnosis of infantile mitochondrial disease with
            targeted next-generation sequencing
  JOURNAL   Sci Transl Med 4 (118), 118ra10 (2012)
   PUBMED   22277967
  REMARK    GeneRIF: Mutations in AARS2 found in lethal mitochondrial myopathy
REFERENCE   8  (bases 1 to 4798)
  AUTHORS   Gotz,A., Tyynismaa,H., Euro,L., Ellonen,P., Hyotylainen,T.,
            Ojala,T., Hamalainen,R.H., Tommiska,J., Raivio,T., Oresic,M.,
            Karikoski,R., Tammela,O., Simola,K.O., Paetau,A., Tyni,T. and
            Suomalainen,A.
  TITLE     Exome sequencing identifies mitochondrial alanyl-tRNA synthetase
            mutations in infantile mitochondrial cardiomyopathy
  JOURNAL   Am J Hum Genet 88 (5), 635-642 (2011)
   PUBMED   21549344
REFERENCE   9  (bases 1 to 4798)
  AUTHORS   Bonnefond,L., Fender,A., Rudinger-Thirion,J., Giege,R., Florentz,C.
            and Sissler,M.
  TITLE     Toward the full set of human mitochondrial aminoacyl-tRNA
            synthetases: characterization of AspRS and TyrRS
  JOURNAL   Biochemistry 44 (12), 4805-4816 (2005)
   PUBMED   15779907
REFERENCE   10 (bases 1 to 4798)
  AUTHORS   Chmiela,T. and Wszolek,Z.K.
  TITLE     AARS2-Related Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   39480987
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353588.25.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_020745.3.
            
            Summary: The protein encoded by this gene belongs to the class-II
            aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play
            critical roles in mRNA translation by charging tRNAs with their
            cognate amino acids. The encoded protein is a mitochondrial enzyme
            that specifically aminoacylates alanyl-tRNA. Mutations in this gene
            are a cause of combined oxidative phosphorylation deficiency 8.
            [provided by RefSeq, Dec 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.55287.1,
                                           SRR1803613.100991.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000244571.5/
                                                  ENSP00000244571.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-267               AL353588.25        36432-36698         c
            268-459             AL353588.25        35423-35614         c
            460-605             AL353588.25        34741-34886         c
            606-773             AL353588.25        34345-34512         c
            774-918             AL353588.25        33650-33794         c
            919-1064            AL353588.25        30600-30745         c
            1065-1173           AL353588.25        30274-30382         c
            1174-1212           AL353588.25        29845-29883         c
            1213-1324           AL353588.25        29631-29742         c
            1325-1458           AL353588.25        29004-29137         c
            1459-1603           AL353588.25        28405-28549         c
            1604-1776           AL353588.25        27996-28168         c
            1777-1890           AL353588.25        27771-27884         c
            1891-2031           AL353588.25        27532-27672         c
            2032-2169           AL353588.25        26637-26774         c
            2170-2279           AL353588.25        26417-26526         c
            2280-2388           AL353588.25        26153-26261         c
            2389-2511           AL353588.25        25742-25864         c
            2512-2622           AL353588.25        25411-25521         c
            2623-2706           AL353588.25        24732-24815         c
            2707-2817           AL353588.25        24507-24617         c
            2818-4798           AL353588.25        22082-24062         c
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..4798
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="alanyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:57505"
                     /db_xref="HGNC:HGNC:21022"
                     /db_xref="MIM:612035"
     exon            1..267
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..2982
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /EC_number="6.1.1.7"
                     /note="alanyl-tRNA synthetase like; alanine tRNA ligase 2,
                     mitochondrial (putative); probable alanyl-tRNA synthetase,
                     mitochondrial; protein lactyltransferase AARS2;
                     alanyl-tRNA synthetase 2, mitochondrial (putative)"
                     /codon_start=1
                     /product="alanine--tRNA ligase, mitochondrial"
                     /protein_id="NP_065796.2"
                     /db_xref="CCDS:CCDS34464.1"
                     /db_xref="GeneID:57505"
                     /db_xref="HGNC:HGNC:21022"
                     /db_xref="MIM:612035"
                     /translation="MAASVAAAARRLRRAIRRSPAWRGLSHRPLSSEPPAAKASAVRA
                     AFLNFFRDRHGHRLVPSASVRPRGDPSLLFVNAGMNQFKPIFLGTVDPRSEMAGFRRV
                     ANSQKCVRAGGHHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACNMAWELLTQVY
                     GIPEERLWISYFDGDPKAGLDPDLETRDIWLSLGVPASRVLSFGPQENFWEMGDTGPC
                     GPCTEIHYDLAGGVGAPQLVELWNLVFMQHNREADGSLQPLPQRHVDTGMGLERLVAV
                     LQGKHSTYDTDLFSPLLNAIQQGCRAPPYLGRVGVADEGRTDTAYRVVADHIRTLSVC
                     ISDGIFPGMSGPPLVLRRILRRAVRFSMEILKAPPGFLGSLVPVVVETLGDAYPELQR
                     NSAQIANLVSEDEAAFLASLERGRRIIDRTLRTLGPSDMFPAEVAWSLSLCGDLGLPL
                     DMVELMLEEKGVQLDSAGLERLAQEEAQHRARQAEPVQKQGLWLDVHALGELQRQGVP
                     PTDDSPKYNYSLRPSGSYEFGTCEAQVLQLYTEDGTAVASVGKGQRCGLLLDRTNFYA
                     EQGGQASDRGYLVRAGQEDVLFPVARAQVCGGFILHEAVAPECLRLGDQVQLHVDEAW
                     RLGCMAKHTATHLLNWALRQTLGPGTEQQGSHLNPEQLRLDVTTQTPLTPEQLRAVEN
                     TVQEAVGQDEAVYMEEVPLALTAQVPGLRSLDEVYPDPVRVVSVGVPVAHALDPASQA
                     ALQTSVELCCGTHLLRTGAVGDLVIIGDRQLSKGTTRLLAVTGEQAQQARELGQSLAQ
                     EVKAATERLSLGSRDVAEALRLSKDIGRLIEAVETAVMPQWQRRELLATVKMLQRRAN
                     TAIRKLQMGQAAKKTQELLERHSKGPLIVDTVSAESLSVLVKVVRQLCEQAPSTSVLL
                     LSPQPMGKVLCACQVAQGAMPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGSTTDLEA
                     ALSIAQTYALSQL"
     exon            268..459
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            460..605
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            606..773
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            774..918
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1064
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1173
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1212
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1324
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1458
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1603
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1776
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1890
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2031
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2169
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2170..2279
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2280..2388
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2511
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2622
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2706
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2817
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..4798
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3840
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="major polyA site"
     regulatory      4541..4546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="hexamer: ATTAAA"
     polyA_site      4561
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="major polyA site"
     regulatory      4780..4785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="hexamer: AGTAAA"
     polyA_site      4798
                     /gene="AARS2"
                     /gene_synonym="AARSL; COXPD8; LKENP; MT-ALARS; MTALARS"
                     /note="major polyA site"
ORIGIN      
        1 gtaaagtcac tgcccggagc tacgatggca gcgtcagtgg cagctgcagc ccggaggctg
       61 cggcgggcca ttcgaaggtc gcccgcatgg cggggcctca gccatcggcc gctctcatcg
      121 gagccccctg cagccaaggc ctcggccgtg agggccgcct ttctgaactt ctttcgggac
      181 cgccatggcc accggctggt gccctccgct tccgtgcggc cccgcggcga ccccagtttg
      241 ctttttgtca atgcgggcat gaaccagttc aagccaatct ttctgggcac cgtggatcca
      301 cgaagcgaga tggcaggctt ccgacgtgtg gccaacagcc agaaatgtgt gagagctgga
      361 ggacaccata acgacctgga agatgtgggt cgagaccttt cccatcatac cttctttgaa
      421 atgcttggca attgggcctt tgggggtgaa tattttaagg aggaggcttg taacatggcc
      481 tgggaactgc tgactcaggt ctatgggatc cctgaggaaa ggctctggat ctcctacttt
      541 gatggtgacc ccaaggcagg gctggaccca gacctggaga ccagggacat ctggctgagc
      601 ttaggggtgc ctgctagccg tgtgctttcc tttggaccac aagagaactt ctgggagatg
      661 ggggatactg gcccttgtgg gccctgtact gagatccact acgaccttgc tggtggggtg
      721 ggagcccccc agctggtaga gctttggaac ctggtcttca tgcaacacaa cagagaggca
      781 gatggaagcc tgcagcccct gccccagcgg catgtggaca caggaatggg cctggaaagg
      841 ctggtggctg tgctgcaagg caaacactcc acctatgaca ctgacctctt ttccccgctg
      901 ctcaacgcca tacagcaggg ctgcagggca cccccttact tgggccgagt aggggtggca
      961 gacgaggggc gcacagacac agcgtaccgc gtggtggctg accacatccg cacactcagt
     1021 gtctgcatct ctgatggcat cttccctggg atgtcaggtc ccccgctggt tcttcgtcgg
     1081 atcctgcgtc gagctgtgcg tttctccatg gagatcttaa aggcaccacc tggcttccta
     1141 ggcagcctgg tacctgtagt ggtggagaca ctgggagatg cttatccaga actgcaaagg
     1201 aactcagccc agatcgccaa cctggtgtca gaggacgagg cagccttcct ggcctccctg
     1261 gagcggggta ggcggatcat tgatcggact ctgaggaccc tggggccttc agatatgttc
     1321 cctgctgaag tggcctggtc cttgtcactg tgtggagacc tgggactccc cttggacatg
     1381 gtagagctga tgctggagga gaaaggggtc cagctagact ccgctggact ggagcggttg
     1441 gcccaagagg aggcccagca ccgggcacgg caggctgagc cagttcagaa gcagggattg
     1501 tggcttgatg tccatgcgct tggggagctg cagcgccaag gagtgccccc aactgacgac
     1561 agccccaagt acaactactc cctgcgaccc agcggaagtt atgagttcgg cacctgtgag
     1621 gcccaggtgt tgcaactgta tacagaggac gggacagcag tggcctccgt ggggaaaggc
     1681 cagcgctgtg gcctcctctt ggacaggacc aacttctacg cagaacaggg gggccaggct
     1741 tcagaccgtg gctacctggt gcgggcaggg caagaggacg tgctgttccc agtagcccgg
     1801 gcccaggtct gtggaggttt catcctgcat gaggcagtag cccctgagtg cctgcggtta
     1861 ggggaccagg tgcagctgca tgtggatgag gcctggcgtc taggctgcat ggcgaagcat
     1921 acggccaccc acctgctgaa ctgggcactg aggcagaccc tgggccctgg cacagagcag
     1981 cagggctccc atctcaatcc tgagcagctg cgcttggatg tgaccaccca gaccccattg
     2041 accccagagc agctccgggc agtggagaac actgtgcagg aggccgtggg gcaggatgag
     2101 gctgtgtaca tggaggaggt gcccctggcg ctcactgccc aggtccctgg cctgcgctct
     2161 ctggatgagg tttacccaga ccctgtgcgg gtggtatcag tgggggtgcc cgtggcccat
     2221 gcattggacc cagcctccca agccgcactg cagacctctg tggagctatg ctgtgggacg
     2281 cacctgttac gtactggggc tgtaggggac ctggttatca tcggggaccg ccagctttcc
     2341 aagggcacta cccgcctgct ggccgtcact ggggagcagg cccagcaggc ccgagagcta
     2401 ggccagagcc tggcccagga agtgaaagcg gccactgagc ggctgagtct ggggagccgg
     2461 gatgtggcgg aggcactgag gctgtccaag gacataggac gactcattga agctgtggaa
     2521 actgctgtga tgccccagtg gcagcggcgg gagctgctgg ccacagtgaa gatgctgcag
     2581 cggcgtgcca acactgccat ccgtaagctg caaatgggac aggctgcaaa gaaaactcag
     2641 gagctgctgg agcggcactc gaaggggcct ctgattgtgg acacagtctc tgctgagtct
     2701 ctctcagtgc tggtgaaggt ggtacggcag ctgtgtgagc aggcccccag cacgtctgtg
     2761 ctcctactca gcccccagcc catggggaag gtgctgtgtg cctgtcaggt ggcccagggt
     2821 gccatgccca ccttcacagc agaggcctgg gcactggcag tgtgcagcca catggggggc
     2881 aaggcgtggg gctcacgagt ggtggcccaa ggcaccggaa gcactactga cctggaagct
     2941 gccctcagta tagcccaaac ctatgccctc agccagctct gacccaggtc cgcccaggga
     3001 cccacatgga ctaaaggcaa tgccaggagc cctgaaggag ccagcagaac cttcccacat
     3061 gctgaagcga ggactagagg ctggaggcca agcagctgag ccaaagaaga tcacctgggc
     3121 cagggcctac gtgacacaag gagacatggg ctagggaagg cagaactggg cccaaagaca
     3181 cacaccccct tggtgaaaga gaagacatgg acaattatgg ctgtgactgc acattagaaa
     3241 ttatttcact ggccgggtgt ggtggttcac acctgtaatc caagcacttt gggaggccga
     3301 ggcaggtaga tcatgaggtc aggagatcaa aaccatcctg gctaacatgg tgaaacccca
     3361 tctctactaa aaatacaaaa aattagccgg gtgtggtggc acacgcctgt agtcccagct
     3421 actcgggagg ctgaggcaga agaatcgctt gaacctggga ggtggaggtt gcagtgggcc
     3481 aagatcgcac cactgcactc cagcctgggt gacagagcga gactctgtct caaataatta
     3541 tttcacagag gatagcaagc tccagaaggt tctattacct ttaaaaaaga gtagttttgc
     3601 ccctcccagc aacctaagac cagtctgaac tcacactgtc tgaaaggcca cccaggatcc
     3661 aaatcgggcc atcttcattg ccatgagtgt tcggacaggc tctgcgtgcc aggcaacagt
     3721 gggcagacaa agggctccct ggctactggg aagatacaga accatcctgt gaaaatcatt
     3781 tcagcactgg agaccttctt tgctattact tctttcatta acaatgacct ctaatttaaa
     3841 ttgggccctg ctggtttgtt gatgaattga gcaactgaga acgtggcggg cactaaactg
     3901 acagttcctt ccttcatgga gcctgcttca tgtggaggaa acaggaaata aagcaaaaga
     3961 agagggtcag gcttggtggc tcatgcctat aatcctggca ttttaggagg ctgaggttgg
     4021 agaatcgctt gaggccagaa gtgcaagacc agcctgcgca aacagcaaga ccctgtctac
     4081 aaaaaaaaaa aaaagtaatc tttatgcctc agcctcccag gtagctgaga ctacccacac
     4141 cttggtccca gctacttggg aggctgaggt gggaaaatca ctttgcccag gaattcaagg
     4201 ccgcagtgag ctatgattgc accactgcac tccaggcaac agagtgagac cctgtcttaa
     4261 aaaaaagaag ggagaaagtg tcagatggtg atgaggtctg ggggggaaat agagaatggg
     4321 gatcaggagt gtggatggtg atattccctc accaagaggt gacatgtgag cagggagctg
     4381 ggaggtgagg gtgtgacccg tgtggaaatc agggaaaagc attccagcct gagggaacag
     4441 ccaatgcaga ggccgtgagg tggccagtgc cactgagcag tgagctggga taggggcagg
     4501 tgaggaggct ggagagcggg gtcagacaaa caatatgctt attaaaacaa gttgtacagc
     4561 acctgcctca agcctgagct gaagctgaaa attggcactt caaagcagga ggaagcagag
     4621 atggaggaaa gccctccttc cctaccagaa tgagtccacc tcagacagcc agactttgga
     4681 cctctgcctt tgttcctaga tctctgttgc tagatgaagg aaagggcctc aggggatcca
     4741 tcttcaaatc aagggctccc tggctaccaa attctgtcta gtaaaaatta ttcgagca
//
