LOCUS       NM_001271874            2417 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens AAR2 splicing factor (AAR2), transcript variant 2,
            mRNA.
ACCESSION   NM_001271874
VERSION     NM_001271874.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Preussner,M., Santos,K.F., Alles,J., Heroven,C., Heyd,F., Wahl,M.C.
            and Weber,G.
  TITLE     Structural and functional investigation of the human snRNP assembly
            factor AAR2 in complex with the RNase H-like domain of PRPF8
  JOURNAL   Acta Crystallogr D Struct Biol 78 (Pt 11), 1373-1383 (2022)
   PUBMED   36322420
  REMARK    GeneRIF: Structural and functional investigation of the human snRNP
            assembly factor AAR2 in complex with the RNase H-like domain of
            PRPF8.
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Fan,L., Gao,B., Xu,Y., Flynn,N., Le,B., You,C., Li,S., Achkar,N.,
            Manavella,P.A., Yang,Z. and Chen,X.
  TITLE     Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts
            in microRNA biogenesis
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2208415119 (2022)
   PUBMED   36191209
  REMARK    GeneRIF: Arabidopsis AAR2, a conserved splicing factor in
            eukaryotes, acts in microRNA biogenesis.
            Erratum:[Proc Natl Acad Sci U S A. 2022 Dec 27;119(52):e2219264119.
            doi: 10.1073/pnas.2219264119.. PMID: 36534814]
REFERENCE   3  (bases 1 to 2417)
  AUTHORS   Klimesova,K., Vojackova,J., Radivojevic,N., Vandermoere,F.,
            Bertrand,E., Verheggen,C. and Stanek,D.
  TITLE     TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation
  JOURNAL   Nat Commun 12 (1), 3646 (2021)
   PUBMED   34131137
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2417)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2417)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 2417)
  AUTHORS   Santos,K., Preussner,M., Heroven,A.C. and Weber,G.
  TITLE     Crystallization and biochemical characterization of the human
            spliceosomal Aar2-Prp8(RNaseH) complex
  JOURNAL   Acta Crystallogr F Struct Biol Commun 71 (Pt 11), 1421-1428 (2015)
   PUBMED   26527271
REFERENCE   7  (bases 1 to 2417)
  AUTHORS   Gevaert,K., Goethals,M., Martens,L., Van Damme,J., Staes,A.,
            Thomas,G.R. and Vandekerckhove,J.
  TITLE     Exploring proteomes and analyzing protein processing by mass
            spectrometric identification of sorted N-terminal peptides
  JOURNAL   Nat Biotechnol 21 (5), 566-569 (2003)
   PUBMED   12665801
REFERENCE   8  (bases 1 to 2417)
  AUTHORS   Gottschalk,A., Kastner,B., Luhrmann,R. and Fabrizio,P.
  TITLE     The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected
            protein composition and includes the splicing factor Aar2p
  JOURNAL   RNA 7 (11), 1554-1565 (2001)
   PUBMED   11720285
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY019727.1 and BC001751.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001271874.1.
            
            Summary: This gene encodes the homolog of the yeast A1-alpha2
            repressin protein that is involved in mRNA splicing. Alternately
            spliced transcript variants have been found for this gene.
            [provided by RefSeq, Dec 2012].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.369361.1,
                                           SRR1660805.154005.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000320849.9/ ENSP00000313674.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                HY019727.1         63-74
            13-2417             BC001751.1         1-2405
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..2417
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /note="AAR2 splicing factor"
                     /db_xref="GeneID:25980"
                     /db_xref="HGNC:HGNC:15886"
                     /db_xref="MIM:617365"
     exon            1..26
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /inference="alignment:Splign:2.1.0"
     exon            27..831
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /inference="alignment:Splign:2.1.0"
     CDS             75..1229
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /note="AAR2 splicing factor homolog"
                     /codon_start=1
                     /product="protein AAR2 homolog"
                     /protein_id="NP_001258803.1"
                     /db_xref="CCDS:CCDS13273.1"
                     /db_xref="GeneID:25980"
                     /db_xref="HGNC:HGNC:15886"
                     /db_xref="MIM:617365"
                     /translation="MAAVQMDPELAKRLFFEGATVVILNMPKGTEFGIDYNSWEVGPK
                     FRGVKMIPPGIHFLHYSSVDKANPKEVGPRMGFFLSLHQRGLTVLRWSTLREEVDLSP
                     APESEVEAMRANLQELDQFLGPYPYATLKKWISLTNFISEATVEKLQPENRQICAFSD
                     VLPVLSMKHTKDRVGQNLPRCGIECKSYQEGLARLPEMKPRAGTEIRFSELPTQMFPE
                     GATPAEITKHSMDLSYALETVLNKQFPSSPQDVLGELQFAFVCFLLGNVYEAFEHWKR
                     LLNLLCRSEAAMMKHHTLYINLISILYHQLGEIPADFFVDIVSQDNFLTSTLQVFFSS
                     ACSIAVDATLRKKAEKFQAHLTKKFRWDFAAEPEDCAPVVVELPEGIEMG"
     exon            832..1061
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..2417
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2394..2399
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /note="hexamer: ATTAAA"
     polyA_site      2417
                     /gene="AAR2"
                     /gene_synonym="C20orf4; CGI-23"
                     /note="major polyA site"
ORIGIN      
        1 gctgtaccgg aacgtggggc gaggcgctgt tcatcaaaga aaaagggttc ttttggtcac
       61 ccaccactgg ccccatggct gccgtgcaga tggatcctga gctagccaag cgcctcttct
      121 ttgaaggggc cactgtggtc atcctgaaca tgcccaaggg aacagagttt gggattgact
      181 ataactcctg ggaggtcggg cccaagttcc ggggcgtgaa gatgatccct ccaggcatcc
      241 acttcctcca ctacagctct gtggacaagg ctaatccgaa ggaagtaggc cctcgtatgg
      301 gtttcttcct tagcctgcac cagcgggggc tgacagtgct gcgctggagc acactcaggg
      361 aagaggtaga cctgtcccca gccccagagt ctgaggtgga ggccatgagg gccaacctcc
      421 aggagctgga ccagttcctg gggccttacc catatgccac cctgaagaag tggatctcac
      481 tcaccaactt catcagcgaa gccacagtgg agaagctaca gcccgagaat cgacagatct
      541 gtgccttttc cgatgtgcta cctgtgctct ccatgaagca caccaaggac cgcgtggggc
      601 agaatctacc ccgctgtggc attgagtgca aaagctacca agagggcctg gcccggctac
      661 cagagatgaa gcccagagcc gggacagaga tccgcttctc agagctgccc acgcagatgt
      721 tcccagaggg tgccacgcca gctgagataa ccaagcacag catggacctg agctatgccc
      781 tggagactgt gctcaacaag cagttcccca gcagccccca ggatgtgctt ggtgaactcc
      841 agtttgcttt tgtgtgcttc ctgctgggga atgtgtacga ggcatttgag cattggaagc
      901 ggctcctgaa cctcctgtgc cggtcagaag cagccatgat gaagcaccac accctctaca
      961 tcaacctcat ctccatcctg taccaccagc ttggtgagat ccccgctgac ttcttcgtag
     1021 acattgtctc ccaagacaac ttcctcacca gcaccttaca ggttttcttt tcctctgcct
     1081 gcagcattgc cgtggatgcc accctgagaa agaaagctga aaagttccaa gctcacctga
     1141 ccaagaagtt ccggtgggac tttgctgcgg aacctgagga ctgtgccccg gtggtggtgg
     1201 agctccctga gggcatcgag atgggctaac tcggggagcg ctctcagctg cgaggggccc
     1261 cttcccacag ggctgcagtc ctggcctctc catttacttc ttcccatcct gggacctgcc
     1321 agggcagcaa tctctccagg tcctgcaaag atggagccag aattcccttt ttcactgata
     1381 aatatatttc ttcattgcca aagaggctgt acccatcctg aaggcacatt tgtgggttcc
     1441 ccatcagcca ggccttggtg ctaacctggc tgaatttcac acaggctctt acacacacac
     1501 gctcctagga gacatctgcc tacacggcaa ccatatttcc tctgaatgag aaggaattga
     1561 accaaaagtc caagaaagaa ctgattgctt gttccatagg agcttaggaa acaagaaacc
     1621 ctggattgcc cagggggtct gagaagttgg ttggtgactt tttttgcggt taaatgaagg
     1681 gtgatgggga gatcagcccg aattgccgcc tgcctcttgc taaataggag cagaggactt
     1741 ggcctgcagc tccttgggag cccttgattg ggaagagagt ttcaagggag gcagctggat
     1801 tcaatctagc aggtggtcag cttcagcttt ctccatcgaa atcccattct cctgtccaga
     1861 ggcccagtgg gtcatctccc aaggtgggtg tggaccctgg cctcagaggc cttgctggtg
     1921 ctgtcacctc ccacctgttc cattccgagg cctcacccag aagtgggacc ctccccttcc
     1981 tcaccagagc caccgtgact gtttctgatg acctggagag tcaacaacaa ccagaaaggt
     2041 ttctgcccag agcaggcttc ttaaggcctt tacgaagttt tgtgccttcc aagtgctgaa
     2101 gaagacctgg tcagcctaaa tcttcccagt cccgctgtgg agctgtcagt caccggagta
     2161 atgagctcct ggttcctcgg gagtccttcg tgctgtgtgg cagggttcct ctctagacaa
     2221 gtacacaggc cctgccaccc tgacatcaaa ctgttgtact atgatcacag tccctgtgcc
     2281 atccttttcc aagactgggg ctcacaccat gtttttgaat gagaatccct gctggttgag
     2341 acttttgctt ccacttgttt ccttggagat gtttttccaa gagcataatg tacattaaag
     2401 tcttcgagtt gagacaa
//
