LOCUS       NM_023928               3256 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant
            1, mRNA.
ACCESSION   NM_023928
VERSION     NM_023928.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Haydar,S., Grigorescu,F., Vintila,M., Cogne,Y., Lautier,C.,
            Tutuncu,Y., Brun,J.F., Robine,J.M., Pugeat,M., Normand,C.,
            Poucheret,P., Gheorghiu,M.L., Georgescu,C., Badiu,C., Baculescu,N.,
            Renard,E., Ylli,D., Badiou,S., Sutra,T., Cristol,J.P., Mercier,J.,
            Gomis,R., Macias,J.M., Litvinov,S., Khusnutdinova,E., Poiana,C.,
            Pasquali,R., Lauro,D., Sesti,G., Prudente,S., Trischitta,V.,
            Tsatsoulis,A., Abdelhak,S., Barakat,A., Zenati,A., Ylli,A.,
            Satman,I., Kanninen,T., Rinato,Y. and Missoni,S.
  TITLE     Fine-scale haplotype mapping of MUT, AACS, SLC6A15 and PRKCA genes
            indicates association with insulin resistance of metabolic syndrome
            and relationship with branched chain amino acid metabolism or
            regulation
  JOURNAL   PLoS One 14 (3), e0214122 (2019)
   PUBMED   30913280
  REMARK    GeneRIF: Fine-scale haplotype mapping of MUT, AACS, SLC6A15 and
            PRKCA genes indicates association with insulin resistance of
            metabolic syndrome and relationship with branched chain amino acid
            metabolism or regulation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Aguilo,F., Camarero,N., Relat,J., Marrero,P.F. and Haro,D.
  TITLE     Transcriptional regulation of the human acetoacetyl-CoA synthetase
            gene by PPARgamma
  JOURNAL   Biochem J 427 (2), 255-264 (2010)
   PUBMED   20102333
  REMARK    GeneRIF: the human AACS promoter is a PPARgamma target gene and
            this nuclear receptor is recruited to the AACS promoter by direct
            interaction with Sp1
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3256)
  AUTHORS   Watkins,P.A., Maiguel,D., Jia,Z. and Pevsner,J.
  TITLE     Evidence for 26 distinct acyl-coenzyme A synthetase genes in the
            human genome
  JOURNAL   J Lipid Res 48 (12), 2736-2750 (2007)
   PUBMED   17762044
REFERENCE   4  (bases 1 to 3256)
  AUTHORS   Ohgami,M., Takahashi,N., Yamasaki,M. and Fukui,T.
  TITLE     Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone
            body-utilizing enzyme, in human brain
  JOURNAL   Biochem Pharmacol 65 (6), 989-994 (2003)
   PUBMED   12623130
REFERENCE   5  (bases 1 to 3256)
  AUTHORS   Gu,T., Orita,S. and Han,M.
  TITLE     Caenorhabditis elegans SUR-5, a novel but conserved protein,
            negatively regulates LET-60 Ras activity during vulval induction
  JOURNAL   Mol Cell Biol 18 (8), 4556-4564 (1998)
   PUBMED   9671465
REFERENCE   6  (bases 1 to 3256)
  AUTHORS   Bergstrom,J.D. and Edmond,J.
  TITLE     A radiochemical assay for acetoacetyl-CoA synthetase
  JOURNAL   Anal Biochem 149 (2), 358-364 (1985)
   PUBMED   4073493
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC040490.1 and BC051862.1.
            
            On Nov 23, 2018 this sequence version replaced NM_023928.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040490.1, SRR1803614.224335.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316519.11/ ENSP00000324842.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-501               BC040490.1         57-557
            502-2446            BC051862.1         476-2420
            2447-3256           BC040490.1         2503-3312
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..3256
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="acetoacetyl-CoA synthetase"
                     /db_xref="GeneID:65985"
                     /db_xref="HGNC:HGNC:21298"
                     /db_xref="MIM:614364"
     exon            1..283
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..2169
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /EC_number="6.2.1.16"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homolog of C. elegans supressor of ras 5 (sur-5);
                     acetoacetate-CoA ligase; acyl-CoA synthetase family member
                     1; protein sur-5 homolog"
                     /codon_start=1
                     /product="acetoacetyl-CoA synthetase isoform 1"
                     /protein_id="NP_076417.2"
                     /db_xref="CCDS:CCDS9263.1"
                     /db_xref="GeneID:65985"
                     /db_xref="HGNC:HGNC:21298"
                     /db_xref="MIM:614364"
                     /translation="MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLAL
                     ESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYA
                     ENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYL
                     PNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHN
                     HMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEF
                     EQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVG
                     WMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKA
                     MKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNF
                     SLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYR
                     KAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEV
                     EDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETK
                     GIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF"
     exon            284..387
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            388..508
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            509..622
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            623..720
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            721..835
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            836..917
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1065
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1146
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1271
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1336
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1459
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1573
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1699
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1769
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1828
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..2031
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..3256
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3229..3234
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="hexamer: AATAAA"
     polyA_site      3256
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="major polyA site"
ORIGIN      
        1 agtcccttgt tccccgccgc cgccgtcgct gacccagccc gccaggcgct cctgaccgtc
       61 gcttcctccg gtcccaggtc cccggccctc gcctcagccc cggcccctgg tccccagccc
      121 tcgtcgcagc cccggccgcc cgccgccgcc atgtccaagg aggagcgccc cggtcgggag
      181 gagatcctgg agtgccaggt gatgtgggag cctgacagta agaagaacac gcagatggac
      241 cgcttccggg cggctgtggg cgccgcctgc ggcctggcgc tggagagtta tgatgacttg
      301 taccattggt ccgttgagtc atattcagac ttctgggcag agttctggaa attcagtgga
      361 attgtcttct cacgtgtgta tgatgaggtt gtggacacat cgaaaggaat cgcagatgtc
      421 cccgagtggt tcaaaggcag tcggctcaac tatgcagaaa acctcctgcg gcacaaagag
      481 aatgacagag ttgcccttta cattgcaagg gaaggcaaag aggaaattgt gaaggtgact
      541 tttgaagagc tgaggcaaga agtggctttg tttgcagcag caatgaggaa aatgggtgtg
      601 aagaaaggag atcgggttgt tggttattta cccaacagtg agcacgctgt cgaggcgatg
      661 ctggctgcgg caagcattgg tgccatctgg agctccacgt ccccggactt cggtgtgaat
      721 ggtgtgctgg accggttttc tcaaattcag ccaaagctca tcttctctgt ggaggctgtt
      781 gtctataatg gcaaagagca caaccacatg gaaaagctgc agcaggtggt taaaggccta
      841 ccagacttga agaaagtggt ggtgattcct tatgtgtcct ccagagagaa catagacctt
      901 tcaaagattc caaacagtgt gtttctggat gactttcttg ccaccggcac cagtgagcag
      961 gccccgcagc tggagttcga gcagctgccc ttcagccacc cactgttcat catgttctca
     1021 tcgggcacca cgggcgcacc caagtgcatg gtgcattccg ctgggggcac cctcatccag
     1081 catctgaagg agcacctgct gcacggcaac atgaccagca gtgacatcct cctgtgctac
     1141 accacggtcg gctggatgat gtggaactgg atggtgtccc ttctggccac aggagcggcc
     1201 atggtcttgt acgatggctc ccccctggtg cccacgccca atgtgctctg ggacctggtt
     1261 gacaggatag gcatcactgt cctggtaact ggggccaagt ggctgtcagt gctggaagag
     1321 aaggccatga agccggtgga aacccacagt ctccagatgc tccacacgat cctgtccact
     1381 ggctccccac tgaaagccca gagctacgag tatgtctaca ggtgcatcaa gagcagcatc
     1441 ctcctgggct ccatctcagg aggcaccgac atcatctcct gcttcatggg ccacaatttt
     1501 tctcttcctg tgtataaagg ggagattcag gcccggaacc tgggcatggc cgtggaagcg
     1561 tggaacgagg aaggaaaggc ggtctgggga gagagcggcg agctggtgtg tactaagccg
     1621 atcccttgcc agcccacaca cttctggaac gatgagaacg gcaacaagta caggaaggcg
     1681 tatttctcca aattcccagg tatctgggct catggcgact actgcagaat caaccccaag
     1741 accgggggca tcgtcatgct tggccggagt gacggcaccc tcaaccccaa cggggtgcgg
     1801 ttcggcagct cggaaatcta taacattgtg gaatccttcg aggaggtgga ggacagcctg
     1861 tgtgtccccc agtataacaa gtacagggag gagagggtga tcctcttcct gaagatggcc
     1921 tccgggcacg ccttccagcc tgacttggtt aagaggatcc gtgacgccat ccgcatgggc
     1981 ttgtctgcgc gacacgtgcc cagcctcatc ctggaaacca agggcatccc gtatacgctc
     2041 aacggcaaga aagtggaagt tgccgtcaaa cagatcatcg ctggaaaagc cgtggagcaa
     2101 ggaggtgctt tctcgaaccc cgagaccctg gatctgtacc gggacatccc tgagctgcag
     2161 ggcttctgag tcagactggc tggcgtgtca ctcagccgca cccgtgtgca ctgtaacttt
     2221 tgtgtgctca agaaattata cagaaaccta cagctgttgt aaaaggatgc tcgcaccaag
     2281 tgttctgtag gcttggggag ggatcgtttc tctgttttgt taaatctggt gggtacctgg
     2341 atcttccaca cgagtgggat tctggccttc agagaccagg agggagtgtc tgggccgcag
     2401 gtgtggcact gtggtgagag tgtgtgtctt tgcacacaca gtgcagcggg aacggtgggg
     2461 ctggctggtg ctgaagacag acacactcct gagccaaggt cttgtcttca acctccccgt
     2521 cccgttgtcc cattttgctc tgtgaaggtg caaatccctt tcttcccttc ccatctcagg
     2581 ctctcctgtt ttccctcagg gtccagtatg cctttgagct ttagctgtta gaaaggaacc
     2641 cccgtgactt gacacagctt tcacagctgg ctgctaggac cggcgggctg ggtgttcacg
     2701 tgtgtctgtg tcatggatgc aatgcaggcc ctggaggact gtgcgtcacc cgtcaaccag
     2761 agcgtgcctc cgggccagct tccctccaag gaatgagtgg atttcataca ggatctcttt
     2821 attgcacaga ctgaatggct ttacatgttt ctaatgtgaa ttaggcatgt gaagcagtgg
     2881 gtgtccaccc gtgtccctca tgggtgagcc ctccagctgt gagcccaggc agtgtggtca
     2941 ccgagtgagg accctcctca ccaggaaccg catccctgtg ctgcctccac ctgagagttg
     3001 ctagggggtt cttgtcgaga tcatgtcatc agcaccccta agtcaagtca cgggtttcca
     3061 tagccaggca gttggtatgt acaattcagt tcagcgtatg aacttgtatc tctaatctga
     3121 tgtccatttt tatatttttt gaaactgagc acaatgaaat cctttcttga atcattttcc
     3181 ttttggatta taaaaatatg ggggaaagtg ctatgatgaa ttttatgcaa taaatgtata
     3241 catgtgtgca catgca
//
