LOCUS       NM_001414677            2740 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens acetoacetyl-CoA synthetase (AACS), transcript variant
            6, mRNA.
ACCESSION   NM_001414677
VERSION     NM_001414677.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Haydar,S., Grigorescu,F., Vintila,M., Cogne,Y., Lautier,C.,
            Tutuncu,Y., Brun,J.F., Robine,J.M., Pugeat,M., Normand,C.,
            Poucheret,P., Gheorghiu,M.L., Georgescu,C., Badiu,C., Baculescu,N.,
            Renard,E., Ylli,D., Badiou,S., Sutra,T., Cristol,J.P., Mercier,J.,
            Gomis,R., Macias,J.M., Litvinov,S., Khusnutdinova,E., Poiana,C.,
            Pasquali,R., Lauro,D., Sesti,G., Prudente,S., Trischitta,V.,
            Tsatsoulis,A., Abdelhak,S., Barakat,A., Zenati,A., Ylli,A.,
            Satman,I., Kanninen,T., Rinato,Y. and Missoni,S.
  TITLE     Fine-scale haplotype mapping of MUT, AACS, SLC6A15 and PRKCA genes
            indicates association with insulin resistance of metabolic syndrome
            and relationship with branched chain amino acid metabolism or
            regulation
  JOURNAL   PLoS One 14 (3), e0214122 (2019)
   PUBMED   30913280
  REMARK    GeneRIF: Fine-scale haplotype mapping of MUT, AACS, SLC6A15 and
            PRKCA genes indicates association with insulin resistance of
            metabolic syndrome and relationship with branched chain amino acid
            metabolism or regulation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Aguilo,F., Camarero,N., Relat,J., Marrero,P.F. and Haro,D.
  TITLE     Transcriptional regulation of the human acetoacetyl-CoA synthetase
            gene by PPARgamma
  JOURNAL   Biochem J 427 (2), 255-264 (2010)
   PUBMED   20102333
  REMARK    GeneRIF: the human AACS promoter is a PPARgamma target gene and
            this nuclear receptor is recruited to the AACS promoter by direct
            interaction with Sp1
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2740)
  AUTHORS   Watkins,P.A., Maiguel,D., Jia,Z. and Pevsner,J.
  TITLE     Evidence for 26 distinct acyl-coenzyme A synthetase genes in the
            human genome
  JOURNAL   J Lipid Res 48 (12), 2736-2750 (2007)
   PUBMED   17762044
REFERENCE   4  (bases 1 to 2740)
  AUTHORS   Ohgami,M., Takahashi,N., Yamasaki,M. and Fukui,T.
  TITLE     Expression of acetoacetyl-CoA synthetase, a novel cytosolic ketone
            body-utilizing enzyme, in human brain
  JOURNAL   Biochem Pharmacol 65 (6), 989-994 (2003)
   PUBMED   12623130
REFERENCE   5  (bases 1 to 2740)
  AUTHORS   Gu,T., Orita,S. and Han,M.
  TITLE     Caenorhabditis elegans SUR-5, a novel but conserved protein,
            negatively regulates LET-60 Ras activity during vulval induction
  JOURNAL   Mol Cell Biol 18 (8), 4556-4564 (1998)
   PUBMED   9671465
REFERENCE   6  (bases 1 to 2740)
  AUTHORS   Bergstrom,J.D. and Edmond,J.
  TITLE     A radiochemical assay for acetoacetyl-CoA synthetase
  JOURNAL   Anal Biochem 149 (2), 358-364 (1985)
   PUBMED   4073493
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122688.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2713669.1,
                                           SRR14038197.1904157.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-283               AC122688.5         118160-118442       c
            284-387             AC122688.5         109898-110001       c
            388-508             AC122688.5         107266-107386       c
            509-622             AC122688.5         97434-97547         c
            623-720             AC122688.5         92354-92451         c
            721-835             AC122688.5         81084-81198         c
            836-917             AC122688.5         80796-80877         c
            918-1065            AC122688.5         76609-76756         c
            1066-1146           AC122688.5         69320-69400         c
            1147-1260           AC122688.5         55603-55716         c
            1261-1386           AC122688.5         54417-54542         c
            1387-1456           AC122688.5         49805-49874         c
            1457-1515           AC122688.5         49025-49083         c
            1516-2740           AC122688.5         40561-41785         c
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..2740
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="acetoacetyl-CoA synthetase"
                     /db_xref="GeneID:65985"
                     /db_xref="HGNC:HGNC:21298"
                     /db_xref="MIM:614364"
     exon            1..283
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..1653
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /EC_number="6.2.1.16"
                     /note="isoform 6 is encoded by transcript variant 6;
                     homolog of C. elegans supressor of ras 5 (sur-5);
                     acetoacetate-CoA ligase; acyl-CoA synthetase family member
                     1; protein sur-5 homolog"
                     /codon_start=1
                     /product="acetoacetyl-CoA synthetase isoform 6"
                     /protein_id="NP_001401606.1"
                     /db_xref="GeneID:65985"
                     /db_xref="HGNC:HGNC:21298"
                     /db_xref="MIM:614364"
                     /translation="MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLAL
                     ESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYA
                     ENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYL
                     PNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHN
                     HMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEF
                     EQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTEA
                     PTSSPASWATIFLFLCIKGRFRPGTWAWPWKRGTRKERRSGERAASWCVLSRSLASPH
                     TSGTMRTATSTGRRISPNSQVSGLMATTAESTPRPGASSCLAGVTAPSTPTGCGSAAR
                     KSITLYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF"
     exon            284..387
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            388..508
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            509..622
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            623..720
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            721..835
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            836..917
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1065
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1146
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1260
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1386
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1456
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1515
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..2740
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2713..2718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="hexamer: AATAAA"
     polyA_site      2740
                     /gene="AACS"
                     /gene_synonym="ACSF1; SUR-5"
                     /note="major polyA site"
ORIGIN      
        1 agtcccttgt tccccgccgc cgccgtcgct gacccagccc gccaggcgct cctgaccgtc
       61 gcttcctccg gtcccaggtc cccggccctc gcctcagccc cggcccctgg tccccagccc
      121 tcgtcgcagc cccggccgcc cgccgccgcc atgtccaagg aggagcgccc cggtcgggag
      181 gagatcctgg agtgccaggt gatgtgggag cctgacagta agaagaacac gcagatggac
      241 cgcttccggg cggctgtggg cgccgcctgc ggcctggcgc tggagagtta tgatgacttg
      301 taccattggt ccgttgagtc atattcagac ttctgggcag agttctggaa attcagtgga
      361 attgtcttct cacgtgtgta tgatgaggtt gtggacacat cgaaaggaat cgcagatgtc
      421 cccgagtggt tcaaaggcag tcggctcaac tatgcagaaa acctcctgcg gcacaaagag
      481 aatgacagag ttgcccttta cattgcaagg gaaggcaaag aggaaattgt gaaggtgact
      541 tttgaagagc tgaggcaaga agtggctttg tttgcagcag caatgaggaa aatgggtgtg
      601 aagaaaggag atcgggttgt tggttattta cccaacagtg agcacgctgt cgaggcgatg
      661 ctggctgcgg caagcattgg tgccatctgg agctccacgt ccccggactt cggtgtgaat
      721 ggtgtgctgg accggttttc tcaaattcag ccaaagctca tcttctctgt ggaggctgtt
      781 gtctataatg gcaaagagca caaccacatg gaaaagctgc agcaggtggt taaaggccta
      841 ccagacttga agaaagtggt ggtgattcct tatgtgtcct ccagagagaa catagacctt
      901 tcaaagattc caaacagtgt gtttctggat gactttcttg ccaccggcac cagtgagcag
      961 gccccgcagc tggagttcga gcagctgccc ttcagccacc cactgttcat catgttctca
     1021 tcgggcacca cgggcgcacc caagtgcatg gtgcattccg ctgggggcac cctcatccag
     1081 catctgaagg agcacctgct gcacggcaac atgaccagca gtgacatcct cctgtgctac
     1141 accacggagg caccgacatc atctcctgct tcatgggcca caatttttct cttcctgtgt
     1201 ataaagggga gattcaggcc cggaacctgg gcatggccgt ggaagcgtgg aacgaggaag
     1261 gaaaggcggt ctggggagag agcggcgagc tggtgtgtac taagccgatc ccttgccagc
     1321 ccacacactt ctggaacgat gagaacggca acaagtacag gaaggcgtat ttctccaaat
     1381 tcccaggtat ctgggctcat ggcgactact gcagaatcaa ccccaagacc gggggcatcg
     1441 tcatgcttgg ccggagtgac ggcaccctca accccaacgg ggtgcggttc ggcagctcgg
     1501 aaatctataa cattgtatac gctcaacggc aagaaagtgg aagttgccgt caaacagatc
     1561 atcgctggaa aagccgtgga gcaaggaggt gctttctcga accccgagac cctggatctg
     1621 taccgggaca tccctgagct gcagggcttc tgagtcagac tggctggcgt gtcactcagc
     1681 cgcacccgtg tgcactgtaa cttttgtgtg ctcaagaaat tatacagaaa cctacagctg
     1741 ttgtaaaagg atgctcgcac caagtgttct gtaggcttgg ggagggatcg tttctctgtt
     1801 ttgttaaatc tggtgggtac ctggatcttc cacacgagtg ggattctggc cttcagagac
     1861 caggagggag tgtctgggcc gcaggtgtgg cactgtggtg agagtgtgtg tctttgcaca
     1921 cacagtgcag cgggaacggt ggggctggct ggtgctgaag acagacacac tcctgagcca
     1981 aggtcttgtc ttcaacctcc ccgtcccgtt gtcccatttt gctctgtgaa ggtgcaaatc
     2041 cctttcttcc cttcccatct caggctctcc tgttttccct cagggtccag tatgcctttg
     2101 agctttagct gttagaaagg aacccccgtg acttgacaca gctttcacag ctggctgcta
     2161 ggaccggcgg gctgggtgtt cacgtgtgtc tgtgtcatgg atgcaatgca ggccctggag
     2221 gactgtgcgt cacccgtcaa ccagagcgtg cctccgggcc agcttccctc caaggaatga
     2281 gtggatttca tacaggatct ctttattgca cagactgaat ggctttacat gtttctaatg
     2341 tgaattaggc atgtgaagca gtgggtgtcc acccgtgtcc ctcatgggtg agccctccag
     2401 ctgtgagccc aggcagtgtg gtcaccgagt gaggaccctc ctcaccagga accgcatccc
     2461 tgtgctgcct ccacctgaga gttgctaggg ggttcttgtc gagatcatgt catcagcacc
     2521 cctaagtcaa gtcacgggtt tccatagcca ggcagttggt atgtacaatt cagttcagcg
     2581 tatgaacttg tatctctaat ctgatgtcca tttttatatt ttttgaaact gagcacaatg
     2641 aaatcctttc ttgaatcatt ttccttttgg attataaaaa tatgggggaa agtgctatga
     2701 tgaattttat gcaataaatg tatacatgtg tgcacatgca
//
