LOCUS       NM_016161               1779 bp    mRNA    linear   PRI 27-JUL-2024
DEFINITION  Homo sapiens alpha-1,4-N-acetylglucosaminyltransferase (A4GNT),
            mRNA.
ACCESSION   NM_016161
VERSION     NM_016161.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Karasawa,F., Shiota,A., Goso,Y., Kobayashi,M., Sato,Y.,
            Masumoto,J., Fujiwara,M., Yokosawa,S., Muraki,T., Miyagawa,S.,
            Ueda,M., Fukuda,M.N., Fukuda,M., Ishihara,K. and Nakayama,J.
  TITLE     Essential role of gastric gland mucin in preventing gastric cancer
            in mice
  JOURNAL   J Clin Invest 122 (3), 923-934 (2012)
   PUBMED   22307328
  REMARK    GeneRIF: human alpha1,4-N-acetylglucosaminyltransferase (alpha4GnT)
            is responsible for O-glycan biosynthesis and has a role in
            preventing gastric cancer by inhibiting H. pylori infection and
            also suppressing tumor-promoting inflammation
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zheng,Z., Jia,Y., Hou,L., Persson,C., Yeager,M., Lissowska,J.,
            Chanock,S.J., Blaser,M., Chow,W.H. and Ye,W.
  TITLE     Genetic variation in a4GnT in relation to Helicobacter pylori
            serology and gastric cancer risk
  JOURNAL   Helicobacter 14 (5), 120-125 (2009)
   PUBMED   19751437
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   3  (bases 1 to 1779)
  AUTHORS   Ishizone,S., Yamauchi,K., Kawa,S., Suzuki,T., Shimizu,F.,
            Harada,O., Sugiyama,A., Miyagawa,S., Fukuda,M. and Nakayama,J.
  TITLE     Clinical utility of quantitative RT-PCR targeted to
            alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of
            pancreatic cancer
  JOURNAL   Cancer Sci 97 (2), 119-126 (2006)
   PUBMED   16441422
  REMARK    GeneRIF: Up-regulation of alpha-1,4-N-acetylglucosaminyltransferase
            is associated with pancreatic cancer
            Erratum:[Cancer Sci. 2006 Mar;97(3):242]
REFERENCE   4  (bases 1 to 1779)
  AUTHORS   Shimizu,F., Nakayama,J., Ishizone,S., Zhang,M.X., Kawakubo,M.,
            Ota,H., Sugiyama,A., Kawasaki,S., Fukuda,M. and Katsuyama,T.
  TITLE     Usefulness of the real-time reverse transcription-polymerase chain
            reaction assay targeted to alpha1,4-N-acetylglucosaminyltransferase
            for the detection of gastric cancer
  JOURNAL   Lab Invest 83 (2), 187-197 (2003)
   PUBMED   12594234
REFERENCE   5  (bases 1 to 1779)
  AUTHORS   Zhang,M.X., Nakayama,J., Hidaka,E., Kubota,S., Yan,J., Ota,H. and
            Fukuda,M.
  TITLE     Immunohistochemical demonstration of
            alpha1,4-N-acetylglucosaminyltransferase that forms
            GlcNAcalpha1,4Galbeta residues in human gastrointestinal mucosa
  JOURNAL   J Histochem Cytochem 49 (5), 587-596 (2001)
   PUBMED   11304796
REFERENCE   6  (bases 1 to 1779)
  AUTHORS   Nakayama,J., Yeh,J.C., Misra,A.K., Ito,S., Katsuyama,T. and
            Fukuda,M.
  TITLE     Expression cloning of a human alpha1,
            4-N-acetylglucosaminyltransferase that forms
            GlcNAcalpha1-->4Galbeta-->R, a glycan specifically expressed in the
            gastric gland mucous cell-type mucin
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 8991-8996 (1999)
   PUBMED   10430883
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC023049.14.
            
            On May 17, 2019 this sequence version replaced NM_016161.2.
            
            Summary: This gene encodes a protein from the glycosyltransferase
            32 family. The enzyme catalyzes the transfer of N-acetylglucosamine
            (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan,
            GlcNAcalpha1-->4Galbeta-->R and is largely associated with the
            Golgi apparatus membrane. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK225668.1, AF141315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142586, SAMEA2146982
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000236709.4/ ENSP00000236709.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC023049.14        76544-76546         c
            4-179               AC023049.14        76368-76543         c
            180-613             AC023049.14        75005-75438         c
            614-1774            AC023049.14        67874-69034         c
            1775-1779           AC023049.14        67869-67873         c
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.3"
     gene            1..1779
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /note="alpha-1,4-N-acetylglucosaminyltransferase"
                     /db_xref="GeneID:51146"
                     /db_xref="HGNC:HGNC:17968"
                     /db_xref="MIM:616709"
     exon            1..179
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /inference="alignment:Splign:2.1.0"
     exon            180..613
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..1228
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /codon_start=1
                     /product="alpha-1,4-N-acetylglucosaminyltransferase"
                     /protein_id="NP_057245.1"
                     /db_xref="CCDS:CCDS3097.1"
                     /db_xref="GeneID:51146"
                     /db_xref="HGNC:HGNC:17968"
                     /db_xref="MIM:616709"
                     /translation="MRKELQLSLSVTLLLVCGFLYQFTLKSSCLFCLPSFKSHQGLEA
                     LLSHRRGIVFLETSERMEPPHLVSCSVESAAKIYPEWPVVFFMKGLTDSTPMPSNSTY
                     PAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINASAERNWLHISSDASRLAIIWK
                     YGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMENFVEHYNSA
                     IWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWD
                     TEPSFNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGE
                     LGPGNK"
     exon            614..1779
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1224..1229
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /note="hexamer: AATAAA"
     polyA_site      1268
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /note="major polyA site"
     regulatory      1401..1406
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /note="hexamer: TATAAA"
     polyA_site      1422
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
     regulatory      1758..1763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
                     /note="hexamer: AATAAA"
     polyA_site      1779
                     /gene="A4GNT"
                     /gene_synonym="alpha4GnT"
ORIGIN      
        1 gtcatttcta agacctcaaa tactggttaa ctgcatttgc agctagaagt taggctctga
       61 ttcactgttt tgtattttct aaaagggtta tatgtaattt gaaagataga cctgccaaga
      121 cgtgagatct gtgttctcct tggttagagc taacattttt ggtgaggaaa gcactgcagg
      181 agcaggctgg cacagagaag aggacatgcg gaaggagctc cagctctccc tgtcagtcac
      241 cttgctgctt gtctgtggct tcctctacca gttcaccctg aagtccagct gcctcttctg
      301 tttgccttct ttcaagtccc accaggggct ggaagccctc ctgagccaca gacgtggcat
      361 tgtgtttcta gagacctcag agagaatgga gccaccccat ttggtctcct gttccgtaga
      421 gtctgctgcc aagatttatc ctgagtggcc tgtggtgttc tttatgaagg gtcttactga
      481 ttccacaccg atgccctcaa actccacata cccagctttt tccttcctgt cagcaataga
      541 caacgttttc ctcttccctt tggatatgaa aaggctgctt gaagacacac cattgttttc
      601 atggtacaat caaatcaacg ccagcgcaga gagaaactgg ctccacatca gctcggatgc
      661 atcccgcctg gccatcatct ggaaatacgg tggcatctac atggacaccg atgtcatctc
      721 catcaggccc atccctgagg agaacttttt ggctgcgcag gcttctcggt actctagtaa
      781 tggaatattt gggttcctcc cccaccaccc ctttttgtgg gaatgcatgg aaaactttgt
      841 tgaacactat aattcagcca tttggggcaa ccaaggccct gagttgatga caaggatgtt
      901 gagggtatgg tgtaaacttg aagacttcca ggaggtgagc gacctcaggt gtctgaacat
      961 atccttctta cacccccaaa gattttaccc catctcctat cgagagtgga ggcgctacta
     1021 tgaagtgtgg gatacagagc caagcttcaa tgtctcttat gccctgcatt tgtggaacca
     1081 catgaaccag gaggggcggg ctgtgattag aggaagcaac acactggtgg aaaatctcta
     1141 tcgcaagcac tgtcccagga cttacaggga cctgattaaa ggcccagagg ggtcagtgac
     1201 tggggagctg ggtccaggta acaaataaag ctaacactcg tttgctgctg ctgcagtgtg
     1261 gaaatggaca tttcctggag tgccacattt tcacttgatc tccactgtct cttggcgggg
     1321 tgggcagagg gttgcctctc aaatgtcagt taggattagc tttctctgtc tgtaatagaa
     1381 aaatcccaaa tgatactggc tataaaacat acacccatgg tccatttgac tgcttgacct
     1441 ccaacccacc atgtttgcat ttcaggcagt aggatgaaga aaagaggaaa ggtgcacttg
     1501 ctttattttg tgaagacttc ctgcaagtac cgtataacac tttctcttac attgcactga
     1561 ctgagatgta gctgcaagag aggctgggaa gtttaaggag gaagaaaaaa tgggtactgg
     1621 gataggcagc taacagtttt gctacatgtt ctatttaata cagtaagatt aattccagga
     1681 cctgttagag cattgactga ggtaaaaaga gacttgattt ctagtcctga ttttgacatt
     1741 cattaatgca ttcagtcaat aaatattaac tgagcctgg
//
