LOCUS       NM_144670               5127 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens alpha-2-macroglobulin like 1 (A2ML1), transcript
            variant 1, mRNA.
ACCESSION   NM_144670
VERSION     NM_144670.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Zhang,X., Tang,C., Lian,J. and Jiang,Y.
  TITLE     A2ML1 Inhibits Esophageal Squamous Cell Carcinoma Progression and
            Serves as a Novel Prognostic Biomarker
  JOURNAL   Can J Gastroenterol Hepatol 2023, 5557546 (2023)
   PUBMED   37954860
  REMARK    GeneRIF: A2ML1 Inhibits Esophageal Squamous Cell Carcinoma
            Progression and Serves as a Novel Prognostic Biomarker.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Santos-Cortez,R.L.P., Ong,K.M.C., Carlos-Hiceta,A., Tantoco,M.L.C.,
            Yarza,T.K.L., San Agustin,M.L., Pedro,M., Cruz,T.L.G., Cutiongco-de
            la Paz,E.M., Abes,G.T., Llanes,E.G.D.V., Chan,A.L., Chiong,C.M. and
            Reyes-Quintos,M.R.T.
  TITLE     Audiologic Measures in an Indigenous Community with A2ML1- and
            FUT2-Related Otitis Media
  JOURNAL   Genet Test Mol Biomarkers 27 (1), 12-17 (2023)
   PUBMED   36719978
  REMARK    GeneRIF: Audiologic Measures in an Indigenous Community with A2ML1-
            and FUT2-Related Otitis Media.
REFERENCE   3  (bases 1 to 5127)
  AUTHORS   Nielsen,N.S., Zarantonello,A., Harwood,S.L., Jensen,K.T., Kjoge,K.,
            Thogersen,I.B., Schauser,L., Karlsen,J.L., Andersen,G.R. and
            Enghild,J.J.
  TITLE     Cryo-EM structures of human A2ML1 elucidate the protease-inhibitory
            mechanism of the A2M family
  JOURNAL   Nat Commun 13 (1), 3033 (2022)
   PUBMED   35641520
  REMARK    GeneRIF: Cryo-EM structures of human A2ML1 elucidate the
            protease-inhibitory mechanism of the A2M family.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5127)
  AUTHORS   Santos-Cortez,R.L., Chiong,C.M., Reyes-Quintos,M.R., Tantoco,M.L.,
            Wang,X., Acharya,A., Abbe,I., Giese,A.P., Smith,J.D., Allen,E.K.,
            Li,B., Cutiongco-de la Paz,E.M., Garcia,M.C., Llanes,E.G.,
            Labra,P.J., Gloria-Cruz,T.L., Chan,A.L., Wang,G.T., Daly,K.A.,
            Shendure,J., Bamshad,M.J., Nickerson,D.A., Patel,J.A.,
            Riazuddin,S., Sale,M.M., Chonmaitree,T., Ahmed,Z.M., Abes,G.T. and
            Leal,S.M.
  CONSRTM   University of Washington Center for Mendelian Genomics
  TITLE     Rare A2ML1 variants confer susceptibility to otitis media
  JOURNAL   Nat Genet 47 (8), 917-920 (2015)
   PUBMED   26121085
  REMARK    GeneRIF: Studies support a role for alpha-2-macroglobulin-like 1
            protein (A2ML1) in the pathophysiology of otitis media.
REFERENCE   5  (bases 1 to 5127)
  AUTHORS   Vissers,L.E., Bonetti,M., Paardekooper Overman,J., Nillesen,W.M.,
            Frints,S.G., de Ligt,J., Zampino,G., Justino,A., Machado,J.C.,
            Schepens,M., Brunner,H.G., Veltman,J.A., Scheffer,H., Gros,P.,
            Costa,J.L., Tartaglia,M., van der Burgt,I., Yntema,H.G. and den
            Hertog,J.
  TITLE     Heterozygous germline mutations in A2ML1 are associated with a
            disorder clinically related to Noonan syndrome
  JOURNAL   Eur J Hum Genet 23 (3), 317-324 (2015)
   PUBMED   24939586
  REMARK    GeneRIF: our results provide evidence that mutations in A2ML1 are a
            cause of Noonan-like syndrome, with a variable phenotype ranging
            from severe to very mild.
REFERENCE   6  (bases 1 to 5127)
  AUTHORS   Numata,S., Teye,K., Tsuruta,D., Sogame,R., Ishii,N., Koga,H.,
            Natsuaki,Y., Tsuchisaka,A., Hamada,T., Karashima,T., Nakama,T.,
            Furumura,M., Ohata,C., Kawakami,T., Schepens,I., Borradori,L. and
            Hashimoto,T.
  TITLE     Anti-alpha-2-macroglobulin-like-1 autoantibodies are detected
            frequently and may be pathogenic in paraneoplastic pemphigus
  JOURNAL   J Invest Dermatol 133 (7), 1785-1793 (2013)
   PUBMED   23407400
REFERENCE   7  (bases 1 to 5127)
  AUTHORS   Schepens,I., Jaunin,F., Begre,N., Laderach,U., Marcus,K.,
            Hashimoto,T., Favre,B. and Borradori,L.
  TITLE     The protease inhibitor alpha-2-macroglobulin-like-1 is the p170
            antigen recognized by paraneoplastic pemphigus autoantibodies in
            human
  JOURNAL   PLoS One 5 (8), e12250 (2010)
   PUBMED   20805888
  REMARK    GeneRIF: a new class of target antigens in a paraneoplastic
            autoimmune multiorgan syndrome
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5127)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   9  (bases 1 to 5127)
  AUTHORS   Galliano,M.F., Toulza,E., Jonca,N., Gonias,S.L., Serre,G. and
            Guerrin,M.
  TITLE     Binding of alpha2ML1 to the low density lipoprotein
            receptor-related protein 1 (LRP1) reveals a new role for LRP1 in
            the human epidermis
  JOURNAL   PLoS One 3 (7), e2729 (2008)
   PUBMED   18648652
  REMARK    GeneRIF: alpha2ML1 binding to the low density lipoprotein
            receptor-related protein 1 (LRP1) reveals a new role for LRP1 in
            the human epidermis
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 5127)
  AUTHORS   Galliano,M.F., Toulza,E., Gallinaro,H., Jonca,N.,
            Ishida-Yamamoto,A., Serre,G. and Guerrin,M.
  TITLE     A novel protease inhibitor of the alpha2-macroglobulin family
            expressed in the human epidermis
  JOURNAL   J Biol Chem 281 (9), 5780-5789 (2006)
   PUBMED   16298998
  REMARK    GeneRIF: alpha2ML1 is the first alpha2macroglobulin family member
            detected in the epidermis, where it may play an important role
            during desquamation by inhibiting extracellular proteases.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006513.37 and AC006581.16.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_144670.5.
            
            Summary: This gene encodes a member of the alpha-macroglobulin
            superfamily. The encoded protein is thought to be an N-glycosylated
            monomeric protein that acts as an inhibitor of several proteases.
            It has been shown to form covalent interactions with proteases, and
            has been reported as the p170 antigen recognized by autoantibodies
            in the autoimmune disease paraneoplastic pemphigus (PNP;
            PMID:20805888). Mutations in these gene have also been associated
            with some cases of Noonan syndrome (NS; PMID:24939586) as well as
            some cases of otitis media (PMID:26121085). Alternative splicing
            results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL832139.1, BX648231.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2147596
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299698.12/ ENSP00000299698.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                AC006513.37        4323-4415
            94-277              AC006513.37        4884-5067
            278-440             AC006513.37        5422-5584
            441-493             AC006513.37        11429-11481
            494-514             AC006513.37        16364-16384
            515-674             AC006513.37        17209-17368
            675-759             AC006513.37        17957-18041
            760-886             AC006513.37        19142-19268
            887-1001            AC006513.37        20038-20152
            1002-1111           AC006513.37        20815-20924
            1112-1279           AC006513.37        23071-23238
            1280-1507           AC006513.37        24836-25063
            1508-1568           AC006513.37        27144-27204
            1569-1714           AC006513.37        27779-27924
            1715-1864           AC006513.37        29251-29400
            1865-2059           AC006513.37        30422-30616
            2060-2150           AC006513.37        31371-31461
            2151-2265           AC006513.37        31862-31976
            2266-2494           AC006513.37        33486-33714
            2495-2621           AC006513.37        33912-34038
            2622-2743           AC006581.16        3085-3206
            2744-2795           AC006581.16        3737-3788
            2796-2879           AC006581.16        4466-4549
            2880-3056           AC006581.16        6121-6297
            3057-3138           AC006581.16        6464-6545
            3139-3295           AC006581.16        6903-7059
            3296-3370           AC006581.16        9838-9912
            3371-3533           AC006581.16        10092-10254
            3534-3748           AC006581.16        12751-12965
            3749-3964           AC006581.16        16799-17014
            3965-4092           AC006581.16        17187-17314
            4093-4183           AC006581.16        17494-17584
            4184-4252           AC006581.16        18092-18160
            4253-4355           AC006581.16        23382-23484
            4356-4397           AC006581.16        23928-23969
            4398-5127           AC006581.16        25015-25744
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..5127
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /note="alpha-2-macroglobulin like 1"
                     /db_xref="GeneID:144568"
                     /db_xref="HGNC:HGNC:23336"
                     /db_xref="MIM:610627"
     exon            1..93
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..4396
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; C3 and PZP-like, alpha-2-macroglobulin domain
                     containing 9; alpha-2-macroglobulin-like protein 1"
                     /codon_start=1
                     /product="alpha-2-macroglobulin-like protein 1 isoform 1
                     precursor"
                     /protein_id="NP_653271.3"
                     /db_xref="CCDS:CCDS8596.2"
                     /db_xref="GeneID:144568"
                     /db_xref="HGNC:HGNC:23336"
                     /db_xref="MIM:610627"
                     /translation="MWAQLLLGMLALSPAIAEELPNYLVTLPARLNFPSVQKVCLDLS
                     PGYSDVKFTVTLETKDKTQKLLEYSGLKKRHLHCISFLVPPPAGGTEEVATIRVSGVG
                     NNISFEEKKKVLIQRQGNGTFVQTDKPLYTPGQQVYFRIVTMDSNFVPVNDKYSMVEL
                     QDPNSNRIAQWLEVVPEQGIVDLSFQLAPEAMLGTYTVAVAEGKTFGTFSVEEYVLPK
                     FKVEVVEPKELSTVQESFLVKICCRYTYGKPMLGAVQVSVCQKANTYWYREVEREQLP
                     DKCRNLSGQTDKTGCFSAPVDMATFDLIGYAYSHQINIVATVVEEGTGVEANATQNIY
                     ISPQMGSMTFEDTSNFYHPNFPFSGKIRVRGHDDSFLKNHLVFLVIYGTNGTFNQTLV
                     TDNNGLAPFTLETSGWNGTDVSLEGKFQMEDLVYNPEQVPRYYQNAYLHLRPFYSTTR
                     SFLGIHRLNGPLKCGQPQEVLVDYYIDPADASPDQEISFSYYLIGKGSLVMEGQKHLN
                     SKKKGLKASFSLSLTFTSRLAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLGF
                     SPSQQLPGAEVELQLQAAPGSLCALRAVDESVLLLRPDRELSNRSVYGMFPFWYGHYP
                     YQVAEYDQCPVSGPWDFPQPLIDPMPQGHSSQRSIIWRPSFSEGTDLFSFFRDVGLKI
                     LSNAKIKKPVDCSHRSPEYSTAMGAGGGHPEAFESSTPLHQAEDSQVRQYFPETWLWD
                     LFPIGNSGKEAVHVTVPDAITEWKAMSFCTSQSRGFGLSPTVGLTAFKPFFVDLTLPY
                     SVVRGESFRLTATIFNYLKDCIRVQTDLAKSHEYQLESWADSQTSSCLCADDAKTHHW
                     NITAVKLGHINFTISTKILDSNEPCGGQKGFVPQKGRSDTLIKPVLVKPEGVLVEKTH
                     SSLLCPKGKVASESVSLELPVDIVPDSTKAYVTVLGDIMGTALQNLDGLVQMPSGCGE
                     QNMVLFAPIIYVLQYLEKAGLLTEEIRSRAVGFLEIGYQKELMYKHSNGSYSAFGERD
                     GNGNTWLTAFVTKCFGQAQKFIFIDPKNIQDALKWMAGNQLPSGCYANVGNLLHTAMK
                     GGVDDEVSLTAYVTAALLEMGKDVDDPMVSQGLRCLKNSATSTTNLYTQALLAYIFSL
                     AGEMDIRNILLKQLDQQAIISGESIYWSQKPTPSSNASPWSEPAAVDVELTAYALLAQ
                     LTKPSLTQKEIAKATSIVAWLAKQHNAYGGFSSTQDTVVALQALAKYATTAYMPSEEI
                     NLVVKSTENFQRTFNIQSVNRLVFQQDTLPNVPGMYTLEASGQGCVYVQTVLRYNILP
                     PTNMKTFSLSVEIGKARCEQPTSPRSLTLTIHTSYVGSRSSSNMAIVEVKMLSGFSPM
                     EGTNQLLLQQPLVKKVEFGTDTLNIYLDELIKNTQTYTFTISQSVLVTNLKPATIKVY
                     DYYLPDEQATIQYSDPCE"
     exon            94..277
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            278..440
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            441..493
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            494..514
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            515..674
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            675..759
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            760..886
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1001
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1111
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1279
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1507
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1568
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1714
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1864
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..2059
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2150
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2265
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2494
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2495..2621
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2743
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2795
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2796..2879
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3056
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3057..3138
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3139..3295
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3296..3370
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3371..3533
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3748
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..3964
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            3965..4092
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            4093..4183
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            4184..4252
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            4253..4355
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4397
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     exon            4398..5127
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5104..5109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /note="hexamer: AATAAA"
     polyA_site      5127
                     /gene="A2ML1"
                     /gene_synonym="CPAMD9; OMS; p170"
                     /note="major polyA site"
ORIGIN      
        1 gaccctggaa aaatctgtct cacccacaaa gatgtgggct cagctccttc taggaatgtt
       61 ggccctatca ccagccattg cagaagaact tccaaactac ctggtgacat taccagcccg
      121 gctaaatttc ccctccgttc agaaggtttg tttggacctg agccctgggt acagtgatgt
      181 taaattcacg gttactctgg agaccaagga caagacccag aagttgctag aatactctgg
      241 actgaagaag aggcacttac attgtatctc ctttcttgta ccacctcctg ctggtggcac
      301 agaagaagtg gccacaatcc gggtgtcggg agttggaaat aacatcagct ttgaggagaa
      361 gaaaaaggtt ctaattcaga ggcaggggaa cggcaccttt gtacagactg acaaacctct
      421 ctacacccca gggcagcaag tgtatttccg cattgtcacc atggatagca acttcgttcc
      481 agtgaatgac aagtactcca tggtggaact acaggatcca aatagcaaca ggattgcaca
      541 gtggctggaa gtggtacctg agcaaggcat tgtagacctg tccttccaac tggcaccaga
      601 ggcaatgctg ggcacctaca ctgtggcagt ggctgagggc aagacctttg gtactttcag
      661 tgtggaggaa tatgtgctgc cgaagtttaa ggtggaagtg gtggaaccca aggagttatc
      721 aacggtgcag gaatctttct tagtaaaaat ttgttgtagg tacacctatg gaaagcccat
      781 gctaggggca gtgcaggtat ctgtgtgtca gaaggcaaat acttactggt atcgagaggt
      841 ggaacgggaa cagcttcctg acaaatgcag gaacctctct ggacagactg acaaaacagg
      901 atgtttctca gcacctgtgg acatggccac ctttgacctc attggatatg cgtacagcca
      961 tcaaatcaat attgtggcta ctgttgtgga ggaagggaca ggtgtggagg ccaatgccac
     1021 tcagaatatc tacatttctc cacaaatggg atcaatgacc tttgaagaca ccagcaattt
     1081 ttaccatcca aatttcccct tcagtgggaa gataagagtt aggggccatg atgactcctt
     1141 cctcaagaac catctagtgt ttctggtgat ttatggcaca aatggaacct tcaaccagac
     1201 cctggttact gataacaatg gcctagctcc ctttaccttg gagacatccg gttggaatgg
     1261 gacagacgtt tctctggagg gaaagtttca aatggaagac ttagtatata atccggaaca
     1321 agtgccacgt tactaccaaa atgcctacct gcacctgcga cccttctaca gcacaacccg
     1381 cagcttcctt ggcatccacc ggctaaacgg ccccttgaaa tgtggccagc cccaggaagt
     1441 gctggtggat tattacatcg acccggccga tgcaagccct gaccaagaga tcagcttctc
     1501 ctactattta atagggaaag gaagtttggt gatggagggg cagaaacacc tgaactctaa
     1561 gaagaaagga ctgaaagcct ccttctctct ctcactgacc ttcacttcga gactggcccc
     1621 tgatccttcc ctggtgatct atgccatttt tcccagtgga ggtgttgtag ctgacaaaat
     1681 tcagttctca gtcgagatgt gctttgacaa tcaggtttcc cttggcttct ccccctccca
     1741 gcagcttcca ggagcagaag tggagctgca gctgcaggca gctcccggat ccctgtgtgc
     1801 gctccgggcg gtggatgaga gtgtcttact gcttaggcca gacagagagc tgagcaaccg
     1861 ctctgtctat gggatgtttc cattctggta tggtcactac ccctatcaag tggctgagta
     1921 tgatcagtgt ccagtgtctg gcccatggga ctttcctcag cccctcattg acccaatgcc
     1981 ccaagggcat tcgagccagc gttccattat ctggaggccc tcgttctctg aaggcacgga
     2041 ccttttcagc tttttccggg acgtgggcct gaaaatactg tccaatgcca aaatcaagaa
     2101 gccagtagat tgcagtcaca gatctccaga atacagcact gctatgggtg caggcggtgg
     2161 tcatccagag gcttttgagt catcaactcc tttacatcaa gcagaggatt ctcaggtccg
     2221 ccagtacttc ccagagacct ggctctggga tctgtttcct attggtaact cggggaagga
     2281 ggcggtccac gtcacagttc ctgacgccat caccgagtgg aaggcgatga gtttctgcac
     2341 ttcccagtca agaggcttcg ggctttcacc cactgttgga ctaactgctt tcaagccgtt
     2401 ctttgttgac ctgactctcc cttactcagt agtccgtggg gaatcctttc gtcttactgc
     2461 caccatcttc aattacctaa aggattgcat cagggttcag actgacctgg ctaaatcgca
     2521 tgagtaccag ctagaatcat gggcagattc tcagacctcc agttgtctct gtgctgatga
     2581 cgcaaaaacc caccactgga acatcacagc tgtcaaattg ggtcacatta actttactat
     2641 tagtacaaag attctggaca gcaatgaacc atgtgggggc cagaaggggt ttgttcccca
     2701 aaagggccga agtgacacgc tcatcaagcc agttctcgtc aaacctgagg gagtcctggt
     2761 ggagaagaca cacagctcat tgctgtgccc aaaaggaaag gtggcatctg aatctgtctc
     2821 cctggagctc ccagtggaca ttgttcctga ctcgaccaag gcttatgtta cggttctggg
     2881 agacattatg ggcacagccc tgcagaacct ggatggtctg gtgcagatgc ccagtggctg
     2941 tggcgagcag aacatggtct tgtttgctcc catcatctat gtcttgcagt acctggagaa
     3001 ggcagggctg ctgacggagg agatcaggtc tcgggcagtg ggtttcctgg aaatagggta
     3061 ccagaaggag ctgatgtaca aacacagcaa tggctcatac agtgcctttg gggagcgaga
     3121 tggaaatgga aacacatggc tgacagcgtt tgtcacaaaa tgctttggcc aagctcagaa
     3181 attcatcttc attgatccca agaacatcca ggatgctctc aagtggatgg caggaaacca
     3241 gctccccagt ggctgctatg ccaacgtggg aaatctcctt cacacagcta tgaagggtgg
     3301 tgttgatgat gaggtctcct tgactgcgta tgtcacagct gcattgctgg agatgggaaa
     3361 ggatgtagat gacccaatgg tgagtcaggg tctacggtgt ctcaagaatt cggccacctc
     3421 cacgaccaac ctctacacac aggccctgtt ggcttacatt ttctccctgg ctggggaaat
     3481 ggacatcaga aacattctcc ttaaacagtt agatcaacag gctatcatct caggagaatc
     3541 catttactgg agccagaaac ctactccatc atcgaacgcc agcccttggt ctgagcctgc
     3601 ggctgtagat gtggaactca cagcatatgc attgttggcc cagcttacca agcccagcct
     3661 gactcaaaag gagatagcga aggccactag catagtggct tggttggcca agcaacacaa
     3721 tgcatatggg ggcttctctt ctactcagga tactgtagtt gctctccaag ctcttgccaa
     3781 atatgccact accgcctaca tgccatctga ggagatcaac ctggttgtaa aatccactga
     3841 gaatttccag cgcacattca acatacagtc agttaacaga ttggtatttc agcaggatac
     3901 cctgcccaat gtccctggaa tgtacacgtt ggaggcctca ggccagggct gtgtctatgt
     3961 gcagacggtg ttgagataca atattctccc tcccacaaat atgaagacct ttagtcttag
     4021 tgtggaaata ggaaaagcta gatgtgagca accgacttca cctcgatcct tgactctcac
     4081 tattcacacc agttatgtgg ggagccgtag ctcttccaat atggctattg tggaagtgaa
     4141 gatgctatct gggttcagtc ccatggaggg caccaatcag ttacttctcc agcaacccct
     4201 ggtgaagaag gttgaatttg gaactgacac acttaacatt tacttggatg agctcattaa
     4261 gaacactcag acttacacct tcaccatcag ccaaagtgtg ctggtcacca acttgaaacc
     4321 agcaaccatc aaggtctatg actactacct accagatgaa caggcaacaa ttcagtattc
     4381 tgatccctgt gaatgaggat aggagctgga aactcaatta gtcctctgtg acatttactg
     4441 gagggtggaa cattcttctg tcgcttgaag cagaactcat tcaatcaaat aatttaattt
     4501 ctctgactag tatatgggta acaaatgaat atgtctgaac ctcagctata atactttcta
     4561 ctacctttgc aaggagatgg gataggaaca atcactcaga ggaggcgttg catgggcagg
     4621 gtcatagggg gaagaaaggt ggtttagctg ttttatttag ccattcaggg ggctctccag
     4681 agaggagacg gtggtagagg gtgaactaga gaagataaga atgtcttcct aggccggatg
     4741 cggtggctca cgcctgtaat cccagcactt tgggattgcg aggtgggcgg atcacttgag
     4801 gtcaggagtt caagaccagc ctggccaaca tggtaaaacc cgtctctact aacaatacaa
     4861 agattagcct ggtgtggtgg cacgggcctg taatcgcagc cccttggaag gccaaggcag
     4921 gagaatcgcc tcaacactgg aggtggaggt tgcagtgagc tgagattgtg ccactgcact
     4981 ccagcctggg caatgaggca agaccctgtc tcaaaaaata ataaataata ataataataa
     5041 tgtttttcta gagtttcagt ctaagggaaa atgtgattta gggctttgga aattggctaa
     5101 aaaaataaaa atggaaaaga aaatctg
//
