LOCUS       NM_000014               4610 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens alpha-2-macroglobulin (A2M), transcript variant 1,
            mRNA.
ACCESSION   NM_000014
VERSION     NM_000014.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   He,T., You,M., Zhu,H., Chen,P. and Fang,S.
  TITLE     CCCTC-binding factor suppresses alpha-2-macroglobulin transcription
            to improve vascular endothelial cell functions in lower extremity
            arteriosclerosis obliterans
  JOURNAL   Gen Physiol Biophys 43 (3), 231-242 (2024)
   PUBMED   38774923
  REMARK    GeneRIF: CCCTC-binding factor suppresses alpha-2-macroglobulin
            transcription to improve vascular endothelial cell functions in
            lower extremity arteriosclerosis obliterans.
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Zhang,Q., Yue,Y., Wang,X., Cui,H., Liu,Y., Gao,M., Liu,T. and
            Xiao,L.
  TITLE     Tandem Mass Tag-Labeled Quantitative Proteome Analyses Identify C1R
            and A2M as Novel Serum Biomarkers in Pregnant Women with
            Obstructive Sleep Apnea
  JOURNAL   J Proteome Res 23 (4), 1232-1248 (2024)
   PUBMED   38407963
  REMARK    GeneRIF: Tandem Mass Tag-Labeled Quantitative Proteome Analyses
            Identify C1R and A2M as Novel Serum Biomarkers in Pregnant Women
            with Obstructive Sleep Apnea.
REFERENCE   3  (bases 1 to 4610)
  AUTHORS   Facchiano,F., D'Arcangelo,D. and Facchiano,A.
  TITLE     Alpha-2-Macroglobulin Is a Novel Anticancer Agent
  JOURNAL   Oncology 102 (7), 641-645 (2024)
   PUBMED   38160662
  REMARK    GeneRIF: Alpha-2-Macroglobulin Is a Novel Anticancer Agent.
REFERENCE   4  (bases 1 to 4610)
  AUTHORS   Neroev,V.V., Chesnokova,N.B., Neroeva,N.V., Beznos,O.V.,
            Pavlenko,T.A., Okhotsimskaya,T.D. and Utkina,O.A.
  TITLE     [Pathogenetic role of multifunctional protein alpha-2-macroglobulin
            and its activity in tears and serum in age-related macular
            degeneration and proliferative diabetic retinopathy]
  JOURNAL   Vestn Oftalmol 139 (6), 26-32 (2023)
   PUBMED   38235627
  REMARK    GeneRIF: [Pathogenetic role of multifunctional protein
            alpha-2-macroglobulin and its activity in tears and serum in
            age-related macular degeneration and proliferative diabetic
            retinopathy].
REFERENCE   5  (bases 1 to 4610)
  AUTHORS   Borth,W.
  TITLE     Alpha 2-macroglobulin, a multifunctional binding protein with
            targeting characteristics
  JOURNAL   FASEB J 6 (15), 3345-3353 (1992)
   PUBMED   1281457
  REMARK    Review article
REFERENCE   6  (bases 1 to 4610)
  AUTHORS   Matthijs,G., Devriendt,K., Cassiman,J.J., Van den Berghe,H. and
            Marynen,P.
  TITLE     Structure of the human alpha-2 macroglobulin gene and its promotor
  JOURNAL   Biochem Biophys Res Commun 184 (2), 596-603 (1992)
   PUBMED   1374237
REFERENCE   7  (bases 1 to 4610)
  AUTHORS   Poller,W., Faber,J.P., Klobeck,G. and Olek,K.
  TITLE     Cloning of the human alpha 2-macroglobulin gene and detection of
            mutations in two functional domains: the bait region and the
            thiolester site
  JOURNAL   Hum Genet 88 (3), 313-319 (1992)
   PUBMED   1370808
REFERENCE   8  (bases 1 to 4610)
  AUTHORS   Marynen,P., Devriendt,K., Van den Berghe,H. and Cassiman,J.J.
  TITLE     A genetic polymorphism in a functional domain of human pregnancy
            zone protein: the bait region. Genomic structure of the bait
            domains of human pregnancy zone protein and alpha 2 macroglobulin
  JOURNAL   FEBS Lett 262 (2), 349-352 (1990)
   PUBMED   1692292
  REMARK    Erratum:[FEBS Lett 1990 Jun 18;266(1-2):195]
REFERENCE   9  (bases 1 to 4610)
  AUTHORS   Jensen,P.E. and Sottrup-Jensen,L.
  TITLE     Primary structure of human alpha 2-macroglobulin. Complete
            disulfide bridge assignment and localization of two interchain
            bridges in the dimeric proteinase binding unit
  JOURNAL   J Biol Chem 261 (34), 15863-15869 (1986)
   PUBMED   2430963
REFERENCE   10 (bases 1 to 4610)
  AUTHORS   Nachman,R.L. and Harpel,P.C.
  TITLE     Platelet alpha2-macroglobulin and alpha1-antitrypsin
  JOURNAL   J Biol Chem 251 (15), 4512-4521 (1976)
   PUBMED   59727
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007436.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_000014.5.
            
            Summary: The protein encoded by this gene is a protease inhibitor
            and cytokine transporter. It uses a bait-and-trap mechanism to
            inhibit a broad spectrum of proteases, including trypsin, thrombin
            and collagenase. It can also inhibit inflammatory cytokines, and it
            thus disrupts inflammatory cascades. Mutations in this gene are a
            cause of alpha-2-macroglobulin deficiency. This gene is implicated
            in Alzheimer's disease (AD) due to its ability to mediate the
            clearance and degradation of A-beta, the major component of
            beta-amyloid deposits. A related pseudogene, which is also located
            on the p arm of chromosome 12, has been identified. [provided by
            RefSeq, Nov 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Both variants 1 and 2 encode isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040071.1, AB209614.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318602.12/ ENSP00000323929.8
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               AC007436.1         30995-31150
            157-340             AC007436.1         33371-33554
            341-500             AC007436.1         34378-34537
            501-553             AC007436.1         34703-34755
            554-574             AC007436.1         36580-36600
            575-743             AC007436.1         36879-37047
            744-828             AC007436.1         37509-37593
            829-949             AC007436.1         39270-39390
            950-1064            AC007436.1         40309-40423
            1065-1174           AC007436.1         40569-40678
            1175-1336           AC007436.1         42514-42675
            1337-1564           AC007436.1         45240-45467
            1565-1628           AC007436.1         45707-45770
            1629-1771           AC007436.1         47391-47533
            1772-1921           AC007436.1         48158-48307
            1922-2083           AC007436.1         51214-51375
            2084-2195           AC007436.1         51830-51941
            2196-2310           AC007436.1         53335-53449
            2311-2539           AC007436.1         55485-55713
            2540-2666           AC007436.1         56432-56558
            2667-2788           AC007436.1         56891-57012
            2789-2840           AC007436.1         57663-57714
            2841-2924           AC007436.1         66737-66820
            2925-3101           AC007436.1         67099-67275
            3102-3189           AC007436.1         67583-67670
            3190-3346           AC007436.1         69057-69213
            3347-3421           AC007436.1         69494-69568
            3422-3602           AC007436.1         69978-70158
            3603-3826           AC007436.1         72131-72354
            3827-4045           AC007436.1         74043-74261
            4046-4173           AC007436.1         74428-74555
            4174-4264           AC007436.1         76336-76426
            4265-4333           AC007436.1         77101-77169
            4334-4436           AC007436.1         78072-78174
            4437-4478           AC007436.1         78690-78731
            4479-4610           AC007436.1         79075-79206
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..4610
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /note="alpha-2-macroglobulin"
                     /db_xref="GeneID:2"
                     /db_xref="HGNC:HGNC:7"
                     /db_xref="MIM:103950"
     exon            1..156
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..4495
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; alpha-2-M; C3 and PZP-like
                     alpha-2-macroglobulin domain-containing protein 5"
                     /codon_start=1
                     /product="alpha-2-macroglobulin isoform a precursor"
                     /protein_id="NP_000005.3"
                     /db_xref="CCDS:CCDS44827.1"
                     /db_xref="GeneID:2"
                     /db_xref="HGNC:HGNC:7"
                     /db_xref="MIM:103950"
                     /translation="MGKNKLLHPSLVLLLLVLLPTDASVSGKPQYMVLVPSLLHTETT
                     EKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMF
                     LTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNE
                     LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHP
                     FTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDAS
                     DCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVE
                     LTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEAN
                     YYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYL
                     VFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRT
                     GTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLAN
                     KVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPE
                     KDLTGFPGPLNDQDNEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSK
                     IRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWI
                     WDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTM
                     PYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTV
                     SWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKET
                     TFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGC
                     GEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGE
                     RYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLN
                     NAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYT
                     KALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSA
                     EVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALS
                     KYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEG
                     CVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNM
                     AIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQD
                     VPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA"
     exon            157..340
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            341..500
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            501..553
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            554..574
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            575..743
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            744..828
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            829..949
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1064
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1174
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1336
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1564
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1628
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1771
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1921
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2083
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2195
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2310
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2539
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2666
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2667..2788
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2840
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2841..2924
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            2925..3101
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3102..3189
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3190..3346
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3347..3421
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3422..3602
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3603..3826
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            3827..4045
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4046..4173
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4174..4264
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4265..4333
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4436
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4437..4478
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     exon            4479..4610
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4585..4590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /note="hexamer: AATAAA"
     polyA_site      4610
                     /gene="A2M"
                     /gene_synonym="A2MD; CPAMD5; FWP007; S863-7"
                     /note="major polyA site"
ORIGIN      
        1 gggaccagat ggattgtagg gagtagggta caatacagtc tgttctcctc cagctccttc
       61 tttctgcaac atggggaaga acaaactcct tcatccaagt ctggttcttc tcctcttggt
      121 cctcctgccc acagacgcct cagtctctgg aaaaccgcag tatatggttc tggtcccctc
      181 cctgctccac actgagacca ctgagaaggg ctgtgtcctt ctgagctacc tgaatgagac
      241 agtgactgta agtgcttcct tggagtctgt caggggaaac aggagcctct tcactgacct
      301 ggaggcggag aatgacgtac tccactgtgt cgccttcgct gtcccaaagt cttcatccaa
      361 tgaggaggta atgttcctca ctgtccaagt gaaaggacca acccaagaat ttaagaagcg
      421 gaccacagtg atggttaaga acgaggacag tctggtcttt gtccagacag acaaatcaat
      481 ctacaaacca gggcagacag tgaaatttcg tgttgtctcc atggatgaaa actttcaccc
      541 cctgaatgag ttgattccac tagtatacat tcaggatccc aaaggaaatc gcatcgcaca
      601 atggcagagt ttccagttag agggtggcct caagcaattt tcttttcccc tctcatcaga
      661 gcccttccag ggctcctaca aggtggtggt acagaagaaa tcaggtggaa ggacagagca
      721 ccctttcacc gtggaggaat ttgttcttcc caagtttgaa gtacaagtaa cagtgccaaa
      781 gataatcacc atcttggaag aagagatgaa tgtatcagtg tgtggcctat acacatatgg
      841 gaagcctgtc cctggacatg tgactgtgag catttgcaga aagtatagtg acgcttccga
      901 ctgccacggt gaagattcac aggctttctg tgagaaattc agtggacagc taaacagcca
      961 tggctgcttc tatcagcaag taaaaaccaa ggtcttccag ctgaagagga aggagtatga
     1021 aatgaaactt cacactgagg cccagatcca agaagaagga acagtggtgg aattgactgg
     1081 aaggcagtcc agtgaaatca caagaaccat aaccaaactc tcatttgtga aagtggactc
     1141 acactttcga cagggaattc ccttctttgg gcaggtgcgc ctagtagatg ggaaaggcgt
     1201 ccctatacca aataaagtca tattcatcag aggaaatgaa gcaaactatt actccaatgc
     1261 taccacggat gagcatggcc ttgtacagtt ctctatcaac accaccaatg ttatgggtac
     1321 ctctcttact gttagggtca attacaagga tcgtagtccc tgttacggct accagtgggt
     1381 gtcagaagaa cacgaagagg cacatcacac tgcttatctt gtgttctccc caagcaagag
     1441 ctttgtccac cttgagccca tgtctcatga actaccctgt ggccatactc agacagtcca
     1501 ggcacattat attctgaatg gaggcaccct gctggggctg aagaagctct ccttctatta
     1561 tctgataatg gcaaagggag gcattgtccg aactgggact catggactgc ttgtgaagca
     1621 ggaagacatg aagggccatt tttccatctc aatccctgtg aagtcagaca ttgctcctgt
     1681 cgctcggttg ctcatctatg ctgttttacc taccggggac gtgattgggg attctgcaaa
     1741 atatgatgtt gaaaattgtc tggccaacaa ggtggatttg agcttcagcc catcacaaag
     1801 tctcccagcc tcacacgccc acctgcgagt cacagcggct cctcagtccg tctgcgccct
     1861 ccgtgctgtg gaccaaagcg tgctgctcat gaagcctgat gctgagctct cggcgtcctc
     1921 ggtttacaac ctgctaccag aaaaggacct cactggcttc cctgggcctt tgaatgacca
     1981 ggacaatgaa gactgcatca atcgtcataa tgtctatatt aatggaatca catatactcc
     2041 agtatcaagt acaaatgaaa aggatatgta cagcttccta gaggacatgg gcttaaaggc
     2101 attcaccaac tcaaagattc gtaaacccaa aatgtgtcca cagcttcaac agtatgaaat
     2161 gcatggacct gaaggtctac gtgtaggttt ttatgagtca gatgtaatgg gaagaggcca
     2221 tgcacgcctg gtgcatgttg aagagcctca cacggagacc gtacgaaagt acttccctga
     2281 gacatggatc tgggatttgg tggtggtaaa ctcagcaggt gtggctgagg taggagtaac
     2341 agtccctgac accatcaccg agtggaaggc aggggccttc tgcctgtctg aagatgctgg
     2401 acttggtatc tcttccactg cctctctccg agccttccag cccttctttg tggagctcac
     2461 aatgccttac tctgtgattc gtggagaggc cttcacactc aaggccacgg tcctaaacta
     2521 ccttcccaaa tgcatccggg tcagtgtgca gctggaagcc tctcccgcct tcctagctgt
     2581 cccagtggag aaggaacaag cgcctcactg catctgtgca aacgggcggc aaactgtgtc
     2641 ctgggcagta accccaaagt cattaggaaa tgtgaatttc actgtgagcg cagaggcact
     2701 agagtctcaa gagctgtgtg ggactgaggt gccttcagtt cctgaacacg gaaggaaaga
     2761 cacagtcatc aagcctctgt tggttgaacc tgaaggacta gagaaggaaa caacattcaa
     2821 ctccctactt tgtccatcag gtggtgaggt ttctgaagaa ttatccctga aactgccacc
     2881 aaatgtggta gaagaatctg cccgagcttc tgtctcagtt ttgggagaca tattaggctc
     2941 tgccatgcaa aacacacaaa atcttctcca gatgccctat ggctgtggag agcagaatat
     3001 ggtcctcttt gctcctaaca tctatgtact ggattatcta aatgaaacac agcagcttac
     3061 tccagagatc aagtccaagg ccattggcta tctcaacact ggttaccaga gacagttgaa
     3121 ctacaaacac tatgatggct cctacagcac ctttggggag cgatatggca ggaaccaggg
     3181 caacacctgg ctcacagcct ttgttctgaa gacttttgcc caagctcgag cctacatctt
     3241 catcgatgaa gcacacatta cccaagccct catatggctc tcccagaggc agaaggacaa
     3301 tggctgtttc aggagctctg ggtcactgct caacaatgcc ataaagggag gagtagaaga
     3361 tgaagtgacc ctctccgcct atatcaccat cgcccttctg gagattcctc tcacagtcac
     3421 tcaccctgtt gtccgcaatg ccctgttttg cctggagtca gcctggaaga cagcacaaga
     3481 aggggaccat ggcagccatg tatataccaa agcactgctg gcctatgctt ttgccctggc
     3541 aggtaaccag gacaagagga aggaagtact caagtcactt aatgaggaag ctgtgaagaa
     3601 agacaactct gtccattggg agcgccctca gaaacccaag gcaccagtgg ggcattttta
     3661 cgaaccccag gctccctctg ctgaggtgga gatgacatcc tatgtgctcc tcgcttatct
     3721 cacggcccag ccagccccaa cctcggagga cctgacctct gcaaccaaca tcgtgaagtg
     3781 gatcacgaag cagcagaatg cccagggcgg tttctcctcc acccaggaca cagtggtggc
     3841 tctccatgct ctgtccaaat atggagcagc cacatttacc aggactggga aggctgcaca
     3901 ggtgactatc cagtcttcag ggacattttc cagcaaattc caagtggaca acaacaaccg
     3961 cctgttactg cagcaggtct cattgccaga gctgcctggg gaatacagca tgaaagtgac
     4021 aggagaagga tgtgtctacc tccagacatc cttgaaatac aatattctcc cagaaaagga
     4081 agagttcccc tttgctttag gagtgcagac tctgcctcaa acttgtgatg aacccaaagc
     4141 ccacaccagc ttccaaatct ccctaagtgt cagttacaca gggagccgct ctgcctccaa
     4201 catggcgatc gttgatgtga agatggtctc tggcttcatt cccctgaagc caacagtgaa
     4261 aatgcttgaa agatctaacc atgtgagccg gacagaagtc agcagcaacc atgtcttgat
     4321 ttaccttgat aaggtgtcaa atcagacact gagcttgttc ttcacggttc tgcaagatgt
     4381 cccagtaaga gatctgaaac cagccatagt gaaagtctat gattactacg agacggatga
     4441 gtttgcaatt gctgagtaca atgctccttg cagcaaagat cttggaaatg cttgaagacc
     4501 acaaggctga aaagtgcttt gctggagtcc tgttctcaga gctccacaga agacacgtgt
     4561 ttttgtatct ttaaagactt gatgaataaa cactttttct ggtcaatgtc
//
