LOCUS       NM_014576               9221 bp    mRNA    linear   PRI 24-APR-2024
DEFINITION  Homo sapiens APOBEC1 complementation factor (A1CF), transcript
            variant 1, mRNA.
ACCESSION   NM_014576
VERSION     NM_014576.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Liu,Y., Yang,J., Weng,D. and Xie,Y.
  TITLE     A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-beta
            Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma
            Cells
  JOURNAL   Int J Mol Sci 25 (7), 3576 (2024)
   PUBMED   38612387
  REMARK    GeneRIF: A1CF Binding to the p65 Interaction Site on NKRF Decreased
            IFN-beta Expression and p65 Phosphorylation (Ser536) in Renal
            Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Liu,Q., Chen,C.Y. and Chen,G.L.
  TITLE     High APOBEC1 Complementation Factor Expression Positively Modulates
            the Proliferation, Invasion, and Migration of Endometrial Cancer
            Cells Through Regulating P53/P21 Signaling Pathway
  JOURNAL   Cancer Biother Radiopharm 37 (9), 750-758 (2022)
   PUBMED   32818382
  REMARK    GeneRIF: High APOBEC1 Complementation Factor Expression Positively
            Modulates the Proliferation, Invasion, and Migration of Endometrial
            Cancer Cells Through Regulating P53/P21 Signaling Pathway.
REFERENCE   3  (bases 1 to 9221)
  AUTHORS   Kawaguchi,M., Nakayama,A., Aoyagi,Y., Nakamura,T., Shimizu,S.,
            Kawamura,Y., Takao,M., Tamura,T., Hishida,A., Nagayoshi,M.,
            Nagase,M., Ooyama,K., Ooyama,H., Shinomiya,N. and Matsuo,H.
  TITLE     Both variants of A1CF and BAZ1B genes are associated with gout
            susceptibility: a replication study and meta-analysis in a Japanese
            population
  JOURNAL   Hum Cell 34 (2), 293-299 (2021)
   PUBMED   33517564
  REMARK    GeneRIF: Both variants of A1CF and BAZ1B genes are associated with
            gout susceptibility: a replication study and meta-analysis in a
            Japanese population.
REFERENCE   4  (bases 1 to 9221)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 9221)
  AUTHORS   Song,Y., Shao,L., Xue,Y., Ruan,X., Liu,X., Yang,C., Zheng,J.,
            Shen,S., Chen,J., Li,Z. and Liu,Y.
  TITLE     Inhibition of the aberrant A1CF-FAM224A-miR-590-3p-ZNF143 positive
            feedback loop attenuated malignant biological behaviors of glioma
            cells
  JOURNAL   J Exp Clin Cancer Res 38 (1), 248 (2019)
   PUBMED   31186064
  REMARK    GeneRIF: this study revealed that A1CF can upregulate the
            expression of FAM224A via strengthening its stability, which in
            turn, influenced the negative modulation of miR-590-3p on
            transcription factor ZNF143.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9221)
  AUTHORS   Blanc,V., Navaratnam,N., Henderson,J.O., Anant,S., Kennedy,S.,
            Jarmuz,A., Scott,J. and Davidson,N.O.
  TITLE     Identification of GRY-RBP as an apolipoprotein B RNA-binding
            protein that interacts with both apobec-1 and apobec-1
            complementation factor to modulate C to U editing
  JOURNAL   J Biol Chem 276 (13), 10272-10283 (2001)
   PUBMED   11134005
REFERENCE   7  (bases 1 to 9221)
  AUTHORS   Chester,A., Scott,J., Anant,S. and Navaratnam,N.
  TITLE     RNA editing: cytidine to uridine conversion in apolipoprotein B
            mRNA
  JOURNAL   Biochim Biophys Acta 1494 (1-2), 1-13 (2000)
   PUBMED   11072063
  REMARK    Review article
REFERENCE   8  (bases 1 to 9221)
  AUTHORS   Yang,Y., Sowden,M.P. and Smith,H.C.
  TITLE     Induction of cytidine to uridine editing on cytoplasmic
            apolipoprotein B mRNA by overexpressing APOBEC-1
  JOURNAL   J Biol Chem 275 (30), 22663-22669 (2000)
   PUBMED   10833526
REFERENCE   9  (bases 1 to 9221)
  AUTHORS   Lellek,H., Kirsten,R., Diehl,I., Apostel,F., Buck,F. and Greeve,J.
  TITLE     Purification and molecular cloning of a novel essential component
            of the apolipoprotein B mRNA editing enzyme-complex
  JOURNAL   J Biol Chem 275 (26), 19848-19856 (2000)
   PUBMED   10781591
REFERENCE   10 (bases 1 to 9221)
  AUTHORS   Mehta,A., Kinter,M.T., Sherman,N.E. and Driscoll,D.M.
  TITLE     Molecular cloning of apobec-1 complementation factor, a novel
            RNA-binding protein involved in the editing of apolipoprotein B
            mRNA
  JOURNAL   Mol Cell Biol 20 (5), 1846-1854 (2000)
   PUBMED   10669759
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF209192.1, AL512366.12,
            AK000324.1 and AL589794.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_014576.3.
            
            Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C
            to U deamination, which is mediated by a multi-component enzyme
            complex containing a minimal core composed of APOBEC-1 and a
            complementation factor encoded by this gene. The gene product has
            three non-identical RNA recognition motifs and belongs to the hnRNP
            R family of RNA-binding proteins. It has been proposed that this
            complementation factor functions as an RNA-binding subunit and
            docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest
            that the protein may also be involved in other RNA editing or RNA
            processing events. Several transcript variants encoding a few
            different isoforms have been found for this gene. [provided by
            RefSeq, Nov 2010].
            
            Transcript Variant: This dominant variant (1), also referred to as
            the wild type, differs in the 5' UTR and coding sequence and lacks
            an alternate in-frame segment compared to variant 5. The resulting
            isoform (1) has a shorter and distinct N-terminus and lacks a short
            internal segment compared to isoform 4. Variants 1, 4, and 7 encode
            the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF209192.1, SRR14038196.3338988.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373997.8/ ENSP00000363109.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AF209192.1         2-51
            51-51               AL512366.12        87083-87083         c
            52-710              AF209192.1         52-710
            711-1957            AK000324.1         853-2099
            1958-9221           AL589794.7         159409-166672       c
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..9221
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="APOBEC1 complementation factor"
                     /db_xref="GeneID:29974"
                     /db_xref="HGNC:HGNC:24086"
                     /db_xref="MIM:618199"
     exon            1..47
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            48..95
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            96..239
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1901
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     APOBEC-1 stimulating protein; apo-B RNA editing protein;
                     apobec-1 complementation factor (ACF) (ASP)"
                     /codon_start=1
                     /product="APOBEC1 complementation factor isoform 1"
                     /protein_id="NP_055391.2"
                     /db_xref="CCDS:CCDS7241.1"
                     /db_xref="GeneID:29974"
                     /db_xref="HGNC:HGNC:24086"
                     /db_xref="MIM:618199"
                     /translation="MESNHKSGDGLSGTQKEAALRALVQRTGYSLVQENGQRKYGGPP
                     PGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVT
                     FSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFVGGIPKTKKREEILSEMKKVT
                     EGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHGIAVDWAEP
                     EVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNR
                     EDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQV
                     YDPTTTYLGAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRG
                     YLAYTGLGRGYQVKGDKREDKLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKN
                     NWGQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPFTPPKLSAFVDEAKTYAAE
                     YTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQLKQAVTLGQDLAAYT
                     TYEVYPTFAVTARGDGYGTF"
     exon            240..374
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            375..505
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            506..744
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            745..909
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1007
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1281
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1463
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1600
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1749
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..9221
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      8940
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="major polyA site"
     regulatory      9199..9204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="hexamer: TATAAA"
     polyA_site      9221
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
ORIGIN      
        1 ataatcaagg aaaccttttc cgggtgggga tctctgaaat tactcagata acagtgctgt
       61 gccaaaaacc tgtggatttt ctctacaaaa attattgagc aaccctaatt aacctgattt
      121 tttgctgata atcactctca atggaatcaa atcacaaatc cggggatgga ttgagcggca
      181 ctcagaagga agcagccctc cgcgcactgg tccagcgcac aggatatagc ttggtccagg
      241 aaaatggaca aagaaaatat ggtggccctc cacctggttg ggatgctgca ccccctgaaa
      301 ggggctgtga aatttttatt ggaaaacttc cccgagacct ttttgaggat gagcttatac
      361 cattatgtga aaaaatcggt aaaatttatg aaatgagaat gatgatggat tttaatggca
      421 acaatagagg atatgcattt gtaacatttt caaataaagt ggaagccaag aatgcaatca
      481 agcaacttaa taattatgaa attagaaatg ggcgcctctt aggggtttgt gccagtgtgg
      541 acaactgccg attatttgtt gggggcatcc caaaaaccaa aaagagagaa gaaatcttat
      601 cggagatgaa aaaggttact gaaggtgttg tcgatgtcat cgtctaccca agcgctgcag
      661 ataaaaccaa aaaccgaggc tttgccttcg tggagtatga gagtcatcga gcagctgcca
      721 tggcgaggag gaaactgcta ccaggaagaa ttcagttatg gggacatggt attgcagtag
      781 actgggcaga gccagaagta gaagttgatg aagatacaat gtcttcagtg aaaatcctat
      841 atgtaagaaa tcttatgctg tctacctctg aagagatgat tgaaaaggaa ttcaacaata
      901 tcaaaccagg tgctgtggag agggtgaaga aaattcgaga ctatgctttt gtgcacttca
      961 gtaaccgaga agatgcagtt gaggctatga aagctttaaa tggcaaggtg ctggatggtt
     1021 cccccattga agtcacccta gcaaaaccag tggacaagga cagttatgtt aggtataccc
     1081 gaggcacagg tggaaggggc accatgctgc aaggagagta tacctactct ttgggccaag
     1141 tttatgatcc caccacaacc taccttggag ctcctgtctt ctatgccccc cagacctatg
     1201 cagcaattcc cagtcttcat ttcccagcca ccaaaggaca tctcagcaac agagccatta
     1261 tccgagcccc ttctgttaga ggggctgcgg gagtgagagg actgggcggc cgtggctatt
     1321 tggcatacac aggcctgggt cgaggatacc aggtcaaagg agacaaaaga gaagacaaac
     1381 tctatgacat tttacctggg atggagctca ccccaatgaa tcctgtcaca ttaaaacccc
     1441 aaggaattaa actcgctccc cagatattag aagagatttg tcagaaaaat aactggggac
     1501 agccagtgta ccagctgcac tctgctattg gacaagacca aagacagcta ttcttgtaca
     1561 aaataactat tcctgctcta gccagccaga atcctgcaat ccaccctttc acacctccaa
     1621 agctgagtgc ctttgtggat gaagcaaaga cgtatgcagc cgaatacacc ctgcagaccc
     1681 tgggcatccc cactgatgga ggcgatggca ccatggctac tgctgctgct gctgctactg
     1741 ctttcccagg atatgctgtc cctaatgcaa ctgcacccgt gtctgcagcc cagctcaagc
     1801 aagcggtaac ccttggacaa gacttagcag catatacaac ctatgaggtc tacccaactt
     1861 ttgcagtgac tgcccgaggg gatggatatg gcaccttctg aagatgcttt tttaaattta
     1921 agaataagac acacaaaact ctattaaaaa aaaaaaagaa ataaacctct aactcggtcc
     1981 ccaatgatca taaataatat gtttcctaaa gaaatgcctt tccagagact gtatagctta
     2041 taccaattat agaatcatga agtaagaacg ttattctgaa atgaaatcaa atgttaaaga
     2101 gccttttgtt taacagaaag ctggagagcc actgccccaa aagttgttct tccagtcttc
     2161 ctgagtgttt ggggagtatg tttacaaatc ctgccagggt gaagagagat cttgtagctg
     2221 gcaagatttg ggagggatgg agcagacctg ggcagtcagt aataatcaag ggaagccaag
     2281 taaactccag agtcttacct ctggctcaca gctgcttttg tgatacacaa agtcacaaag
     2341 aggaaaaggg aagggaggag gttgcttttc aaaatgcatt caccatggct cactgtgtgg
     2401 ggcatgattg caggcctgag ctcatgaaag actgtccaag attttgtggt ttcaacctct
     2461 cttcagcctg aaaattccat ccagtcgatt tgtcacagat caggatcttc ctgtcccctg
     2521 cagcccatgg ggacttccta gaaatagata tgatatgttg tagatgtggt taaaacaaat
     2581 ccaggcccca ttgtttattc agctcattag tccacaccta tttttcccta gaactcctac
     2641 cttttccaat aatcatgcca gcatgaaatg accttttcta ctttaaaatt ttttgacttg
     2701 ttcatattgc ttgattttca tcatctcaga ctcatttttc tcctacctaa tatcataaaa
     2761 aagtaaatca gatatacaga atatcattta tgatacttct tcctttcagc aaattgactg
     2821 aaaagtcatt ttaaagagag aagtgcagat ttgctctcat ttctttgata ttgttgcagt
     2881 atccacccca ccatctagca atcagagatg cttatattaa cttgggatta gctttgcatt
     2941 atctaaccca tttattttaa atctgccagg aaatcctcta actttccttc ctttttgttt
     3001 cagtaagtat caggcagctt caccatacct gagtcctttt gtcttgaagc tgccacagaa
     3061 aaatcttaca gcaatcattg ctgattagaa actgtttcag acaatcagca tgggtgttat
     3121 ttaccaaatt ccccccagag tcctaggcct cttctccaga aatatctgat gatgaagtga
     3181 ggggagggca acggtgctac aaaacacgga acagaggtaa agagaaggca ctactttctt
     3241 gccatacttg taaatgattg ctttgttcaa acataaataa tcttaagtcc aacaccaaat
     3301 acctgttact cctacatcaa tctcattagt ggtttaagac acagtactag aattttcatt
     3361 ttttaaaatc ccttggccct taaaaaaatt tacagaacac aaaggaaaac ataaacacaa
     3421 agacatggaa aattttgtca actccttaat ggaattctgt gatcaaaaag caggccagat
     3481 tctaatcaaa atcaggtaaa ttttaatcac aatcagaagt acttgtaaca tttcagttgt
     3541 cctaactcca atgagataac aaagcctcca aggctacagc tgaaactctg aaaggccctg
     3601 tgctttctac tttacattta gcgtctaata tttcctagga cagtagttcc caaagtaggc
     3661 tgtacattag aatctcctgg agagcttttt aaatgctaat gccaataaca tatctcataa
     3721 aatttacact agaatttcct tggatgggtg cctggcatca gtatttttta atgatcccta
     3781 ggtgatttca atgtgcacct aggtttggga attgctatat tcttgtattt atactctatc
     3841 tctttatttc ttttcctttt tatttcactt ttagtaaata gaaaaatgca attaaaaaat
     3901 catctaagtt aagacaaata gccaaatcat atttatccca taaatagtat gaatttaaat
     3961 gtaacttacg taggcttcaa actaactcta aaaatgagaa agtaaaacaa tcttgtccta
     4021 aaacaattct tagatccaaa tttacactcc cctacagatc tgcttttatt ctcaaagaca
     4081 gtgattacat tgacataaca aatgtccatt ggggcaggca aatttctact gagtctaaaa
     4141 agcattccaa attctaggag caaaagttct gattttcctt aaaatctttg caaataaaca
     4201 gcaaagggta ttctggatgt cttgtcaatg caaattaggt ttgatttaga gaagttttaa
     4261 atttaatcgc aatgaaaaga ttagttaata catgaatctt tttatgagaa aaaatcatta
     4321 ttctggataa agtcataatt gtttgtttac aatattaaga gcagacacct aaacatacaa
     4381 taataccaaa gtattgagaa aattaccact ggaacaccat ctcaagttat cacaccattt
     4441 acctctttag agccagtttt gtataagtag gtagaagtgg ttttggtttt tttgctaaaa
     4501 ttttactttg ttttagtaat atctagggaa tactttgaat tccattaaaa taccttcata
     4561 gaaagtgtta atgtgtctac catgccatat gtttattttt actttcccca agttaggtag
     4621 atagagcaag tcctgctacc ataagtttaa aattattaag acaacgggct tttttaaatt
     4681 gctggtagaa ccaaaggcta attttgcata ggtttatatt tttagactta agaaattagc
     4741 acctatataa taccttcata ccttctgcat cactatcaat ttttctgtaa tttcttattt
     4801 gaatgcttcc taattcttta cagaaaaaat taaaatttgt cacccaacca ggtgataata
     4861 acaaaacaat aatttaggaa catccagtaa atttgaatta gttctctaaa ttttggtaat
     4921 taatagctta ttgataatta aaatcagcct aatgttgctt aagtaggagg aaattgatca
     4981 gcaatgggat tgggtatcta ttttctttcc tataaaagtg ctgcctcttc tcaaattgct
     5041 ttatttctct cccactgcat tagtatatct agaacagcac cttcaactgc aacaaaatat
     5101 aattctccta gatgactagg atatcctcca atctggtatc atgccaaaca acactatgtt
     5161 tggggaacag cacccgaaat gctgctaaaa agctacctca tgtcaagaaa atatggattt
     5221 caaaaaatat ctggcacagt ggcccatgcc tataacccca gtactttggg aggccaaggc
     5281 aggaggattg ctggaggcca ggagttcaag accagcctgg gcaacatgga gagactccgc
     5341 ctctacaaaa aaaaaaaaaa caaatttaat ttaattggcc agttgtggtg gcgtgggcct
     5401 gtggtcccag ctacttggga agctaaggca ggaggattgc tggaggcctg gagggcaagg
     5461 ctgcagtgag ccatgttcac atcgctgcac tccagcctgg gtgacagagc aagaccctgt
     5521 ctccaaaaaa gcaaaaataa atatctaaca aaaaggaacc tgtttccaaa ccacagtttc
     5581 ttaacttttt catgatggaa tagaaattaa actataagta tatactgata atagctgtgc
     5641 aggaaattgg tttttatttt acttaaaagt attttttaga aaaaatgtta ttctgattgc
     5701 atagattagc aaaatattgc atcaatataa tgttccattt ttattaattt ttgttatatt
     5761 ctaaatgacc gttttataat tctgtcttgg acaactgggc cacagttaga atccacatag
     5821 acagggaggt tcttaggtgt gccatttgta gaataaaggg caatcaagtt aatattcggg
     5881 gtaaattttc attgcattct aacctatgaa gtttgataaa gtatattatt attgaacaac
     5941 tctaacacta agatttccta aaagtaactt acaacaaaaa atgaactatg tgctcaatat
     6001 tcacataaag tcatgattat tctcttcttg cattcatata aaagttccca gggggaagaa
     6061 aatatatcta aaaattagac tagaaacttg gcacttttta tgtattgtag atacactttc
     6121 atatattgta aatacatctg ttaaagataa aactaagttt ctaacatagc actacaagga
     6181 aactttggat aaatattttt aaaaagaaaa tgttaatagg ataacttaag attttgtata
     6241 tacttattgg aagacaggaa gaaactaggc agtaaatatt cctacctgac gtgcaaccat
     6301 tgtcaatcag gttttgttaa actgtcaaat ttgattttga aattttagtt tgcccaaata
     6361 atatcttgaa aatgctctga attttactag aacatattca tagaaagtgt aatgtatcta
     6421 ccaggctcta tgtttgtgtt tgccaacatt agaaaaacaa agtaagttgc gctataatga
     6481 gtgaaactaa ggaaaatgag atttttctga ctcccattcc ctctcttaaa aagtaaaata
     6541 tttaagcttt ttttattaaa ggaaaaatgg gtacagaata ctctattgcc ctccaaatgt
     6601 ccaacatttt taaagccatg ttgtccaaca atgtttatac agaaaataga atgcataggt
     6661 ttaatataga tagcaatgta tatgtaggct catttgtggg agattgcaaa ctttatgtgc
     6721 ccccagttct ccttggtttt gtaggaaagc taaaagaaaa ccttgtactc tcactaataa
     6781 tgaaattcat agccaattta gaaagggaaa ataacctgaa aaccaaaata cctcattcat
     6841 ctgaagatgc ctggaataaa atatggaatt cttctgtacc tacctccccc ttacagactt
     6901 ttctcctatt ttgagagaga aattcaagaa gaagaataca gcttgttctc tttccacaaa
     6961 tgatactttg tctaagggaa taggagatac caagaaacag tatctttaaa aaagtaagct
     7021 gcccacaggc cttcttggag aatgaagaat atcagcccca ttcagggttt accaatgtct
     7081 ctctgaggtg acaatacatt ctggttttcc aggacagtcc tggcttaggt ctgctgtcct
     7141 tctataaata ttaacaatga tccctcttat ttccaaacgt gtcccagttt agacaacaaa
     7201 ttatacagtg tctgccccca acacacacac acacacccca ccatctttat gggttgttgt
     7261 tgttttcttg ttgtttttaa ttttgtagaa ttggctttgt cttctccaaa gcagataaca
     7321 gcttaaacta aagagcctag ctacttcttt ctgactgagc agggtcatag taaacagaga
     7381 agaatggatc acttcctcct ctgaaatctc catcaatcca ctttctgtcc aactatctct
     7441 ctacccagcc tatttttggt cctagttttc ctctcataac ccctgggctg tgagtcctga
     7501 gttctgcctt catatggcag agcaagctag tcattattat gccaggttag gtggtggggc
     7561 aagacaaaag atagctcttg gggacacttg cttggtctgt agagatccac agggcactag
     7621 tagggtatta gagcatttat tggtttcttt cttccttgag ttgagttact tctatcccaa
     7681 aacagacagg tcttacgtta accttcacct taaaaaggat attgcctttt ttccttctgc
     7741 ttcacaaatt tgctccaaca ctaattccct tgcatttgat ttcatcatta tgattgttca
     7801 tcaaccattc ttcacgaaaa atcctcacat cacgtcatat aaaggtttta ttatttttaa
     7861 tggaaaataa cattgagaga ttattttcct ttttcataaa atttcccttt ttccctccct
     7921 ctattctctt cactaacatt ggaaattaaa caagttttac aggctctatt gttattaacc
     7981 ctcaaatcta ttcttcagag aatatatgat cagcctgtgt ttactatgca cctactctta
     8041 taccagaaac tgtgaataca aatacatata tttcctactt ctctctttaa ttggggcaca
     8101 cagtcccatg gtccattctg catttttcaa aattgaatat aattgcaaca cctgataggt
     8161 tttgccactt agaattctga aaataaatca actctgttct aaaccttaag tttccactat
     8221 tattgccaaa ctctggactg atacaataag ccttttaaaa ggtgaattta acacttcacc
     8281 tattatggtt atttaagtaa aatgagtttt caaatatttc ccccaggaga aatataaagg
     8341 actatgcaaa aatagaagga gaaaaaggtt agttagaggt ccagaaggag aattaaaaca
     8401 tggaattgat aaattcacct actgatttat gatgttaatg agcaggtacc tatacatttt
     8461 atttacttga aaacattttg gccattaact atttctgttt tctaaataca tttgtgttta
     8521 taaaatttta aaatctatat cacaagactt agacatgcct ttctttgcaa taaagtatat
     8581 ataccttttt acaaatgtaa attttatttt gaaacttggc attttcttta taattcctaa
     8641 gatatctttg ggcacacaga ctacagataa atctatttaa atcaattcat cattatcact
     8701 ggtttaaata tttaatgtaa ccattggaat ttttattctt attccttcag agaattctcc
     8761 ttttcttcta tgtttctgac tgcaatacag cccgatattg ttattcccta tatcttaatg
     8821 aaaagcaagc tgtcagcaaa atgcatttat agtttccaga tgtttaacaa tctctttctt
     8881 cttttctttt ttgttattat ttttatttat gtgaagttag ttagctgagg taccaaaaaa
     8941 gagacaaaga cagtattaaa aagtgtgcat aaagcactta tttttgtact ttagtacaat
     9001 gttttataga actttgtgat ctatctaaat atattaagta aaattacacc attcacttgt
     9061 tgggaaaata atctttggtt tggaagatat taacataatg ggcatcttag aatcataaat
     9121 cacatgaaat gagagacaat gcaatattgt ataattcctg gatgatgcaa ttgttttaat
     9181 tgaattttca agtgccatta taaagtttta aaaattatca a
//
