LOCUS       NM_001198819            9481 bp    mRNA    linear   PRI 24-APR-2024
DEFINITION  Homo sapiens APOBEC1 complementation factor (A1CF), transcript
            variant 5, mRNA.
ACCESSION   NM_001198819
VERSION     NM_001198819.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Liu,Y., Yang,J., Weng,D. and Xie,Y.
  TITLE     A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-beta
            Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma
            Cells
  JOURNAL   Int J Mol Sci 25 (7), 3576 (2024)
   PUBMED   38612387
  REMARK    GeneRIF: A1CF Binding to the p65 Interaction Site on NKRF Decreased
            IFN-beta Expression and p65 Phosphorylation (Ser536) in Renal
            Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Liu,Q., Chen,C.Y. and Chen,G.L.
  TITLE     High APOBEC1 Complementation Factor Expression Positively Modulates
            the Proliferation, Invasion, and Migration of Endometrial Cancer
            Cells Through Regulating P53/P21 Signaling Pathway
  JOURNAL   Cancer Biother Radiopharm 37 (9), 750-758 (2022)
   PUBMED   32818382
  REMARK    GeneRIF: High APOBEC1 Complementation Factor Expression Positively
            Modulates the Proliferation, Invasion, and Migration of Endometrial
            Cancer Cells Through Regulating P53/P21 Signaling Pathway.
REFERENCE   3  (bases 1 to 9481)
  AUTHORS   Kawaguchi,M., Nakayama,A., Aoyagi,Y., Nakamura,T., Shimizu,S.,
            Kawamura,Y., Takao,M., Tamura,T., Hishida,A., Nagayoshi,M.,
            Nagase,M., Ooyama,K., Ooyama,H., Shinomiya,N. and Matsuo,H.
  TITLE     Both variants of A1CF and BAZ1B genes are associated with gout
            susceptibility: a replication study and meta-analysis in a Japanese
            population
  JOURNAL   Hum Cell 34 (2), 293-299 (2021)
   PUBMED   33517564
  REMARK    GeneRIF: Both variants of A1CF and BAZ1B genes are associated with
            gout susceptibility: a replication study and meta-analysis in a
            Japanese population.
REFERENCE   4  (bases 1 to 9481)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 9481)
  AUTHORS   Song,Y., Shao,L., Xue,Y., Ruan,X., Liu,X., Yang,C., Zheng,J.,
            Shen,S., Chen,J., Li,Z. and Liu,Y.
  TITLE     Inhibition of the aberrant A1CF-FAM224A-miR-590-3p-ZNF143 positive
            feedback loop attenuated malignant biological behaviors of glioma
            cells
  JOURNAL   J Exp Clin Cancer Res 38 (1), 248 (2019)
   PUBMED   31186064
  REMARK    GeneRIF: this study revealed that A1CF can upregulate the
            expression of FAM224A via strengthening its stability, which in
            turn, influenced the negative modulation of miR-590-3p on
            transcription factor ZNF143.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9481)
  AUTHORS   Blanc,V., Navaratnam,N., Henderson,J.O., Anant,S., Kennedy,S.,
            Jarmuz,A., Scott,J. and Davidson,N.O.
  TITLE     Identification of GRY-RBP as an apolipoprotein B RNA-binding
            protein that interacts with both apobec-1 and apobec-1
            complementation factor to modulate C to U editing
  JOURNAL   J Biol Chem 276 (13), 10272-10283 (2001)
   PUBMED   11134005
REFERENCE   7  (bases 1 to 9481)
  AUTHORS   Chester,A., Scott,J., Anant,S. and Navaratnam,N.
  TITLE     RNA editing: cytidine to uridine conversion in apolipoprotein B
            mRNA
  JOURNAL   Biochim Biophys Acta 1494 (1-2), 1-13 (2000)
   PUBMED   11072063
  REMARK    Review article
REFERENCE   8  (bases 1 to 9481)
  AUTHORS   Yang,Y., Sowden,M.P. and Smith,H.C.
  TITLE     Induction of cytidine to uridine editing on cytoplasmic
            apolipoprotein B mRNA by overexpressing APOBEC-1
  JOURNAL   J Biol Chem 275 (30), 22663-22669 (2000)
   PUBMED   10833526
REFERENCE   9  (bases 1 to 9481)
  AUTHORS   Lellek,H., Kirsten,R., Diehl,I., Apostel,F., Buck,F. and Greeve,J.
  TITLE     Purification and molecular cloning of a novel essential component
            of the apolipoprotein B mRNA editing enzyme-complex
  JOURNAL   J Biol Chem 275 (26), 19848-19856 (2000)
   PUBMED   10781591
REFERENCE   10 (bases 1 to 9481)
  AUTHORS   Mehta,A., Kinter,M.T., Sherman,N.E. and Driscoll,D.M.
  TITLE     Molecular cloning of apobec-1 complementation factor, a novel
            RNA-binding protein involved in the editing of apolipoprotein B
            mRNA
  JOURNAL   Mol Cell Biol 20 (5), 1846-1854 (2000)
   PUBMED   10669759
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK303801.1, AL512366.12,
            AK291982.1 and AL589794.7.
            
            On Aug 13, 2020 this sequence version replaced NM_001198819.1.
            
            Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C
            to U deamination, which is mediated by a multi-component enzyme
            complex containing a minimal core composed of APOBEC-1 and a
            complementation factor encoded by this gene. The gene product has
            three non-identical RNA recognition motifs and belongs to the hnRNP
            R family of RNA-binding proteins. It has been proposed that this
            complementation factor functions as an RNA-binding subunit and
            docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest
            that the protein may also be involved in other RNA editing or RNA
            processing events. Several transcript variants encoding a few
            different isoforms have been found for this gene. [provided by
            RefSeq, Nov 2010].
            
            Transcript Variant: This variant (5) represents the longest
            transcript and encodes the longest isoform (4).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK303801.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-353               AK303801.1         3-355
            354-354             AL512366.12        73792-73792         c
            355-1819            AK303801.1         357-1821
            1820-2343           AK291982.1         1653-2176
            2344-9481           AL589794.7         159409-166546       c
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q11.23"
     gene            1..9481
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="APOBEC1 complementation factor"
                     /db_xref="GeneID:29974"
                     /db_xref="HGNC:HGNC:24086"
                     /db_xref="MIM:618199"
     exon            1..47
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            48..95
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            96..188
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            189..332
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            333..475
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..2161
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="isoform 4 is encoded by transcript variant 5;
                     APOBEC-1 stimulating protein; apo-B RNA editing protein;
                     apobec-1 complementation factor (ACF) (ASP)"
                     /codon_start=1
                     /product="APOBEC1 complementation factor isoform 4"
                     /protein_id="NP_001185748.1"
                     /db_xref="CCDS:CCDS73133.1"
                     /db_xref="GeneID:29974"
                     /db_xref="HGNC:HGNC:24086"
                     /db_xref="MIM:618199"
                     /translation="MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENG
                     QRKYGGPPPGWDAAPPERGCEIFIGKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGN
                     NRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCASVDNCRLFVGGIPKTKKREEI
                     LSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPGRIQLWGHG
                     IAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDY
                     AFVHFSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGE
                     YTYSLGQVYDPTTTYLGAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVREIYM
                     NVPVGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPGMELTPMNPVTLKPQ
                     GIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALASQNPAIHPFTP
                     PKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAA
                     QLKQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF"
     exon            476..610
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            611..741
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            742..980
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1145
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1243
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1517
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1723
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1860
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            1861..2009
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..9481
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      9200
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="major polyA site"
     regulatory      9459..9464
                     /regulatory_class="polyA_signal_sequence"
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
                     /note="hexamer: TATAAA"
     polyA_site      9481
                     /gene="A1CF"
                     /gene_synonym="ACF; ACF64; ACF65; APOBEC1CF; ASP"
ORIGIN      
        1 ataatcaagg aaaccttttc cgggtgggga tctctgaaat tactcagata acagtgctgt
       61 gccaaaaacc tgtggatttt ctctacaaaa attatcatga ctgttatctt tttgatggaa
      121 aagatgaagt tcagcgaaga acaaatctac aacttggcca gaagactgta gactttgcct
      181 tcaaagagtg agcaacccta attaacctga ttttttgctg ataatcactc tcaatggaat
      241 caaatcacaa atccggggat ggattgagcg gcactcagaa ggaagcagcc ctccgcgcac
      301 tggtccagcg cacaggatat agcttggtcc aggaatttag aggttcctat agatggaagc
      361 agtgtgtctg ggcacatgcc cagagccaga agcgagcatg agcacagcaa ttcctggcct
      421 caaaaaagga aataatgctt tgcagtccat cattttgcaa actttgctgg aaaaggaaaa
      481 tggacaaaga aaatatggtg gccctccacc tggttgggat gctgcacccc ctgaaagggg
      541 ctgtgaaatt tttattggaa aacttccccg agaccttttt gaggatgagc ttataccatt
      601 atgtgaaaaa atcggtaaaa tttatgaaat gagaatgatg atggatttta atggcaacaa
      661 tagaggatat gcatttgtaa cattttcaaa taaagtggaa gccaagaatg caatcaagca
      721 acttaataat tatgaaatta gaaatgggcg cctcttaggg gtttgtgcca gtgtggacaa
      781 ctgccgatta tttgttgggg gcatcccaaa aaccaaaaag agagaagaaa tcttatcgga
      841 gatgaaaaag gttactgaag gtgttgtcga tgtcatcgtc tacccaagcg ctgcagataa
      901 aaccaaaaac cgaggctttg ccttcgtgga gtatgagagt catcgagcag ctgccatggc
      961 gaggaggaaa ctgctaccag gaagaattca gttatgggga catggtattg cagtagactg
     1021 ggcagagcca gaagtagaag ttgatgaaga tacaatgtct tcagtgaaaa tcctatatgt
     1081 aagaaatctt atgctgtcta cctctgaaga gatgattgaa aaggaattca acaatatcaa
     1141 accaggtgct gtggagaggg tgaagaaaat tcgagactat gcttttgtgc acttcagtaa
     1201 ccgagaagat gcagttgagg ctatgaaagc tttaaatggc aaggtgctgg atggttcccc
     1261 cattgaagtc accctagcaa aaccagtgga caaggacagt tatgttaggt atacccgagg
     1321 cacaggtgga aggggcacca tgctgcaagg agagtatacc tactctttgg gccaagttta
     1381 tgatcccacc acaacctacc ttggagctcc tgtcttctat gccccccaga cctatgcagc
     1441 aattcccagt cttcatttcc cagccaccaa aggacatctc agcaacagag ccattatccg
     1501 agccccttct gttagagaaa tttacatgaa tgtacctgta ggggctgcgg gagtgagagg
     1561 actgggcggc cgtggctatt tggcatacac aggcctgggt cgaggatacc aggtcaaagg
     1621 agacaaaaga gaagacaaac tctatgacat tttacctggg atggagctca ccccaatgaa
     1681 tcctgtcaca ttaaaacccc aaggaattaa actcgctccc cagatattag aagagatttg
     1741 tcagaaaaat aactggggac agccagtgta ccagctgcac tctgctattg gacaagacca
     1801 aagacagcta ttcttgtaca aaataactat tcctgctcta gccagccaga atcctgcaat
     1861 ccaccctttc acacctccaa agctgagtgc ctttgtggat gaagcaaaga cgtatgcagc
     1921 cgaatacacc ctgcagaccc tgggcatccc cactgatgga ggcgatggca ccatggctac
     1981 tgctgctgct gctgctactg ctttcccagg atatgctgtc cctaatgcaa ctgcacccgt
     2041 gtctgcagcc cagctcaagc aagcggtaac ccttggacaa gacttagcag catatacaac
     2101 ctatgaggtc tacccaactt ttgcagtgac tgcccgaggg gatggatatg gcaccttctg
     2161 aagatgcttt tttaaattta agaataagac acacaaaact ctattaaaaa aaaaaaagaa
     2221 ataaacctct aactcggtcc ccaatgatca taaataatat gtttcctaaa gaaatgcctt
     2281 tccagagact gtatagctta taccaattat agaatcatga agtaagaacg ttattctgaa
     2341 atgaaatcaa atgttaaaga gccttttgtt taacagaaag ctggagagcc actgccccaa
     2401 aagttgttct tccagtcttc ctgagtgttt ggggagtatg tttacaaatc ctgccagggt
     2461 gaagagagat cttgtagctg gcaagatttg ggagggatgg agcagacctg ggcagtcagt
     2521 aataatcaag ggaagccaag taaactccag agtcttacct ctggctcaca gctgcttttg
     2581 tgatacacaa agtcacaaag aggaaaaggg aagggaggag gttgcttttc aaaatgcatt
     2641 caccatggct cactgtgtgg ggcatgattg caggcctgag ctcatgaaag actgtccaag
     2701 attttgtggt ttcaacctct cttcagcctg aaaattccat ccagtcgatt tgtcacagat
     2761 caggatcttc ctgtcccctg cagcccatgg ggacttccta gaaatagata tgatatgttg
     2821 tagatgtggt taaaacaaat ccaggcccca ttgtttattc agctcattag tccacaccta
     2881 tttttcccta gaactcctac cttttccaat aatcatgcca gcatgaaatg accttttcta
     2941 ctttaaaatt ttttgacttg ttcatattgc ttgattttca tcatctcaga ctcatttttc
     3001 tcctacctaa tatcataaaa aagtaaatca gatatacaga atatcattta tgatacttct
     3061 tcctttcagc aaattgactg aaaagtcatt ttaaagagag aagtgcagat ttgctctcat
     3121 ttctttgata ttgttgcagt atccacccca ccatctagca atcagagatg cttatattaa
     3181 cttgggatta gctttgcatt atctaaccca tttattttaa atctgccagg aaatcctcta
     3241 actttccttc ctttttgttt cagtaagtat caggcagctt caccatacct gagtcctttt
     3301 gtcttgaagc tgccacagaa aaatcttaca gcaatcattg ctgattagaa actgtttcag
     3361 acaatcagca tgggtgttat ttaccaaatt ccccccagag tcctaggcct cttctccaga
     3421 aatatctgat gatgaagtga ggggagggca acggtgctac aaaacacgga acagaggtaa
     3481 agagaaggca ctactttctt gccatacttg taaatgattg ctttgttcaa acataaataa
     3541 tcttaagtcc aacaccaaat acctgttact cctacatcaa tctcattagt ggtttaagac
     3601 acagtactag aattttcatt ttttaaaatc ccttggccct taaaaaaatt tacagaacac
     3661 aaaggaaaac ataaacacaa agacatggaa aattttgtca actccttaat ggaattctgt
     3721 gatcaaaaag caggccagat tctaatcaaa atcaggtaaa ttttaatcac aatcagaagt
     3781 acttgtaaca tttcagttgt cctaactcca atgagataac aaagcctcca aggctacagc
     3841 tgaaactctg aaaggccctg tgctttctac tttacattta gcgtctaata tttcctagga
     3901 cagtagttcc caaagtaggc tgtacattag aatctcctgg agagcttttt aaatgctaat
     3961 gccaataaca tatctcataa aatttacact agaatttcct tggatgggtg cctggcatca
     4021 gtatttttta atgatcccta ggtgatttca atgtgcacct aggtttggga attgctatat
     4081 tcttgtattt atactctatc tctttatttc ttttcctttt tatttcactt ttagtaaata
     4141 gaaaaatgca attaaaaaat catctaagtt aagacaaata gccaaatcat atttatccca
     4201 taaatagtat gaatttaaat gtaacttacg taggcttcaa actaactcta aaaatgagaa
     4261 agtaaaacaa tcttgtccta aaacaattct tagatccaaa tttacactcc cctacagatc
     4321 tgcttttatt ctcaaagaca gtgattacat tgacataaca aatgtccatt ggggcaggca
     4381 aatttctact gagtctaaaa agcattccaa attctaggag caaaagttct gattttcctt
     4441 aaaatctttg caaataaaca gcaaagggta ttctggatgt cttgtcaatg caaattaggt
     4501 ttgatttaga gaagttttaa atttaatcgc aatgaaaaga ttagttaata catgaatctt
     4561 tttatgagaa aaaatcatta ttctggataa agtcataatt gtttgtttac aatattaaga
     4621 gcagacacct aaacatacaa taataccaaa gtattgagaa aattaccact ggaacaccat
     4681 ctcaagttat cacaccattt acctctttag agccagtttt gtataagtag gtagaagtgg
     4741 ttttggtttt tttgctaaaa ttttactttg ttttagtaat atctagggaa tactttgaat
     4801 tccattaaaa taccttcata gaaagtgtta atgtgtctac catgccatat gtttattttt
     4861 actttcccca agttaggtag atagagcaag tcctgctacc ataagtttaa aattattaag
     4921 acaacgggct tttttaaatt gctggtagaa ccaaaggcta attttgcata ggtttatatt
     4981 tttagactta agaaattagc acctatataa taccttcata ccttctgcat cactatcaat
     5041 ttttctgtaa tttcttattt gaatgcttcc taattcttta cagaaaaaat taaaatttgt
     5101 cacccaacca ggtgataata acaaaacaat aatttaggaa catccagtaa atttgaatta
     5161 gttctctaaa ttttggtaat taatagctta ttgataatta aaatcagcct aatgttgctt
     5221 aagtaggagg aaattgatca gcaatgggat tgggtatcta ttttctttcc tataaaagtg
     5281 ctgcctcttc tcaaattgct ttatttctct cccactgcat tagtatatct agaacagcac
     5341 cttcaactgc aacaaaatat aattctccta gatgactagg atatcctcca atctggtatc
     5401 atgccaaaca acactatgtt tggggaacag cacccgaaat gctgctaaaa agctacctca
     5461 tgtcaagaaa atatggattt caaaaaatat ctggcacagt ggcccatgcc tataacccca
     5521 gtactttggg aggccaaggc aggaggattg ctggaggcca ggagttcaag accagcctgg
     5581 gcaacatgga gagactccgc ctctacaaaa aaaaaaaaaa caaatttaat ttaattggcc
     5641 agttgtggtg gcgtgggcct gtggtcccag ctacttggga agctaaggca ggaggattgc
     5701 tggaggcctg gagggcaagg ctgcagtgag ccatgttcac atcgctgcac tccagcctgg
     5761 gtgacagagc aagaccctgt ctccaaaaaa gcaaaaataa atatctaaca aaaaggaacc
     5821 tgtttccaaa ccacagtttc ttaacttttt catgatggaa tagaaattaa actataagta
     5881 tatactgata atagctgtgc aggaaattgg tttttatttt acttaaaagt attttttaga
     5941 aaaaatgtta ttctgattgc atagattagc aaaatattgc atcaatataa tgttccattt
     6001 ttattaattt ttgttatatt ctaaatgacc gttttataat tctgtcttgg acaactgggc
     6061 cacagttaga atccacatag acagggaggt tcttaggtgt gccatttgta gaataaaggg
     6121 caatcaagtt aatattcggg gtaaattttc attgcattct aacctatgaa gtttgataaa
     6181 gtatattatt attgaacaac tctaacacta agatttccta aaagtaactt acaacaaaaa
     6241 atgaactatg tgctcaatat tcacataaag tcatgattat tctcttcttg cattcatata
     6301 aaagttccca gggggaagaa aatatatcta aaaattagac tagaaacttg gcacttttta
     6361 tgtattgtag atacactttc atatattgta aatacatctg ttaaagataa aactaagttt
     6421 ctaacatagc actacaagga aactttggat aaatattttt aaaaagaaaa tgttaatagg
     6481 ataacttaag attttgtata tacttattgg aagacaggaa gaaactaggc agtaaatatt
     6541 cctacctgac gtgcaaccat tgtcaatcag gttttgttaa actgtcaaat ttgattttga
     6601 aattttagtt tgcccaaata atatcttgaa aatgctctga attttactag aacatattca
     6661 tagaaagtgt aatgtatcta ccaggctcta tgtttgtgtt tgccaacatt agaaaaacaa
     6721 agtaagttgc gctataatga gtgaaactaa ggaaaatgag atttttctga ctcccattcc
     6781 ctctcttaaa aagtaaaata tttaagcttt ttttattaaa ggaaaaatgg gtacagaata
     6841 ctctattgcc ctccaaatgt ccaacatttt taaagccatg ttgtccaaca atgtttatac
     6901 agaaaataga atgcataggt ttaatataga tagcaatgta tatgtaggct catttgtggg
     6961 agattgcaaa ctttatgtgc ccccagttct ccttggtttt gtaggaaagc taaaagaaaa
     7021 ccttgtactc tcactaataa tgaaattcat agccaattta gaaagggaaa ataacctgaa
     7081 aaccaaaata cctcattcat ctgaagatgc ctggaataaa atatggaatt cttctgtacc
     7141 tacctccccc ttacagactt ttctcctatt ttgagagaga aattcaagaa gaagaataca
     7201 gcttgttctc tttccacaaa tgatactttg tctaagggaa taggagatac caagaaacag
     7261 tatctttaaa aaagtaagct gcccacaggc cttcttggag aatgaagaat atcagcccca
     7321 ttcagggttt accaatgtct ctctgaggtg acaatacatt ctggttttcc aggacagtcc
     7381 tggcttaggt ctgctgtcct tctataaata ttaacaatga tccctcttat ttccaaacgt
     7441 gtcccagttt agacaacaaa ttatacagtg tctgccccca acacacacac acacacccca
     7501 ccatctttat gggttgttgt tgttttcttg ttgtttttaa ttttgtagaa ttggctttgt
     7561 cttctccaaa gcagataaca gcttaaacta aagagcctag ctacttcttt ctgactgagc
     7621 agggtcatag taaacagaga agaatggatc acttcctcct ctgaaatctc catcaatcca
     7681 ctttctgtcc aactatctct ctacccagcc tatttttggt cctagttttc ctctcataac
     7741 ccctgggctg tgagtcctga gttctgcctt catatggcag agcaagctag tcattattat
     7801 gccaggttag gtggtggggc aagacaaaag atagctcttg gggacacttg cttggtctgt
     7861 agagatccac agggcactag tagggtatta gagcatttat tggtttcttt cttccttgag
     7921 ttgagttact tctatcccaa aacagacagg tcttacgtta accttcacct taaaaaggat
     7981 attgcctttt ttccttctgc ttcacaaatt tgctccaaca ctaattccct tgcatttgat
     8041 ttcatcatta tgattgttca tcaaccattc ttcacgaaaa atcctcacat cacgtcatat
     8101 aaaggtttta ttatttttaa tggaaaataa cattgagaga ttattttcct ttttcataaa
     8161 atttcccttt ttccctccct ctattctctt cactaacatt ggaaattaaa caagttttac
     8221 aggctctatt gttattaacc ctcaaatcta ttcttcagag aatatatgat cagcctgtgt
     8281 ttactatgca cctactctta taccagaaac tgtgaataca aatacatata tttcctactt
     8341 ctctctttaa ttggggcaca cagtcccatg gtccattctg catttttcaa aattgaatat
     8401 aattgcaaca cctgataggt tttgccactt agaattctga aaataaatca actctgttct
     8461 aaaccttaag tttccactat tattgccaaa ctctggactg atacaataag ccttttaaaa
     8521 ggtgaattta acacttcacc tattatggtt atttaagtaa aatgagtttt caaatatttc
     8581 ccccaggaga aatataaagg actatgcaaa aatagaagga gaaaaaggtt agttagaggt
     8641 ccagaaggag aattaaaaca tggaattgat aaattcacct actgatttat gatgttaatg
     8701 agcaggtacc tatacatttt atttacttga aaacattttg gccattaact atttctgttt
     8761 tctaaataca tttgtgttta taaaatttta aaatctatat cacaagactt agacatgcct
     8821 ttctttgcaa taaagtatat ataccttttt acaaatgtaa attttatttt gaaacttggc
     8881 attttcttta taattcctaa gatatctttg ggcacacaga ctacagataa atctatttaa
     8941 atcaattcat cattatcact ggtttaaata tttaatgtaa ccattggaat ttttattctt
     9001 attccttcag agaattctcc ttttcttcta tgtttctgac tgcaatacag cccgatattg
     9061 ttattcccta tatcttaatg aaaagcaagc tgtcagcaaa atgcatttat agtttccaga
     9121 tgtttaacaa tctctttctt cttttctttt ttgttattat ttttatttat gtgaagttag
     9181 ttagctgagg taccaaaaaa gagacaaaga cagtattaaa aagtgtgcat aaagcactta
     9241 tttttgtact ttagtacaat gttttataga actttgtgat ctatctaaat atattaagta
     9301 aaattacacc attcacttgt tgggaaaata atctttggtt tggaagatat taacataatg
     9361 ggcatcttag aatcataaat cacatgaaat gagagacaat gcaatattgt ataattcctg
     9421 gatgatgcaa ttgttttaat tgaattttca agtgccatta taaagtttta aaaattatca
     9481 a
//
